Binder CB-S 170, CB-S 170-UL, CB-S 260, CB-S 260-UL Operating Manual
Operating Manual
CB-S / CB-S-UL (E7)
CO2 Incubator
with FPI-sensor system and RD4 controller
Model Model version Art. No.
CB-S 170 CBS170-230V 9040-0189, 9140-0189
CB-S 170-UL CBSUL170-120V 9040-0190, 9140-0190
CB-S 260 CBS260-230V 9040-0191, 9140-0191
CB-S 260-UL CBSUL260-120V 9040-0192, 9140-0192
BINDER GmbH
Address: Post office box 102, 78502 Tuttlingen, Germany Phone: +49 7462 2005 0
Fax: +49 7462 2005 100 Internet: http://www .b ind er-world.com
E-mail: info@binder-world.com Service Hotline: +49 7462 2005 555
Service Fax: +49 7462 2005 93 555 Service E-Mail: service@binder-world.com
Service Hotline USA: +1 866 885 9794 or +1 631 224 4340 x3
Service Hotline Asia Pacif ic: +852 390 705 04 or +852 390 705 03
Service Hotline Russia and CIS: +7 495 988 15 16
Issue 06/2020 Art. No. 7001-0402
Contents
1. SAFETY .................................................................................................................. 6
1.1 Personnel Qualification ....................................................................................................................... 6
1.2 Operating manual ................................................................................................................................ 6
1.3 Legal considerations ........................................................................................................................... 6
1.4 Structure of the safety instructions ...................................................................................................... 7
1.4.1 Signal word panel ..................................................................................................................... 7
1.4.2 Safety alert symbol ................................................................................................................... 7
1.4.3 Pictograms ................................................................................................................................ 8
1.4.4 Word message panel structure ................................................................................................. 8
1.5 Localization / position of safety labels at the chamber ....................................................................... 9
1.6 Type plate.......................................................................................................................................... 10
1.7 General safety instructions on installing and operating the chamber ............................................... 11
1.8 Precautions when working with CO2 gas .......................................................................................... 13
1.9 Precautions when handling gas cylinders ......................................................................................... 13
1.10 Intended use ..................................................................................................................................... 14
1.11 Foreseeable Misuse .......................................................................................................................... 15
1.12 Residual Risks .................................................................................................................................. 16
1.13 Operating instructions ....................................................................................................................... 17
1.14 Measures to prevent accidents ......................................................................................................... 18
2. CHAMBER DESCRIPTION .................................................................................. 19
2.1 Chamber overview ............................................................................................................................ 20
2.2 Inner chamber ................................................................................................................................... 21
2.3 Connection panel on the rear of the chamber................................................................................... 22
2.4 Chamber doors ................................................................................................................................. 23
2.5 Instrument panel ............................................................................................................................... 23
3. COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND
INSTALLATION .................................................................................................... 24
3.1 Unpacking, and checking equipment and completeness of delivery ................................................ 24
3.2 Guidelines for safe lifting and transportation ..................................................................................... 25
3.3 Storage .............................................................................................................................................. 25
3.4 Location of installation and ambient conditions ................................................................................ 25
4. INSTALLATION AND CONNECTIONS ............................................................... 28
4.1 Shelves .............................................................................................................................................. 28
4.2 CO2 sensor ........................................................................................................................................ 28
4.2.1 Connecting the CO2 sensor .................................................................................................... 28
4.2.2 General notes ......................................................................................................................... 28
4.3 Water pan .......................................................................................................................................... 29
4.4 Gas connection ................................................................................................................................. 30
4.4.1 Connecting the CO2 gas cylinder ............................................................................................ 31
4.4.2 Connecting the gas hose to the chamber rear ....................................................................... 32
4.4.3 Gas cylinder connection kit (option) ........................................................................................ 33
4.5 Electrical connection ......................................................................................................................... 34
5. FUNCTIONAL OVERVIEW OF THE RD4 CHAMBER CONTROLLER ............... 35
5.1 Menu structure of the controller and access levels ........................................................................... 36
5.2 Performance during and after power failures and sh ut do wn ........................................................... 37
6. START UP ............................................................................................................ 37
6.1 Turning on the chamber .................................................................................................................... 37
6.2 Preset factory parameters ................................................................................................................. 38
6.3 Performance after turning on the chamber ....................................................................................... 38
6.4 Altitude of the installation site above sea level ................................................................................. 39
CB-S / CB-S-UL (E7) 06/2020 page 2/119
7. TEMPERATURE AND CO2 SET-POINT ENTRY ................................................. 40
7.1 Temperature set-point entry .............................................................................................................. 40
7.2 CO2 set-point entry ............................................................................................................................ 40
8. PLACING SAMPLES IN THE INCUBATOR ........................................................ 41
9. SETTING SPECIAL CONTROLLER FUNCTIONS .............................................. 42
9.1 Idle mode........................................................................................................................................... 42
9.2 Deactivated CO2 control .................................................................................................................... 43
10. PASSWORD ......................................................................................................... 43
10.1 Password request ............................................................................................................................. 43
10.2 Assign and modify a password ......................................................................................................... 44
10.2.1 Assign and modify the User password ................................................................................... 44
10.2.2 Assign and modify the Admin password ................................................................................. 44
11. TEMPERATURE SAFETY DEVICES ................................................................... 45
11.1 Over temperature protective device (class 1) ................................................................................... 45
11.2 Overtemperature safety controller class 3.1 ..................................................................................... 45
11.2.1 Setting the safety controller mode .......................................................................................... 46
11.2.2 Setting the safety controller value ........................................................................................... 46
11.2.3 Message and measures in the state of alarm ......................................................................... 47
11.2.4 Function check ........................................................................................................................ 47
12. GENERAL CONTROLLER SETTINGS ................................................................ 48
12.1 Selecting the controller’s menu language ......................................................................................... 48
12.2 Selecting the temperature unit .......................................................................................................... 48
12.3 Setting the current date ..................................................................................................................... 49
12.4 Setting the current time ..................................................................................................................... 50
12.5 Function “Language selection at restart” .......................................................................................... 50
12.6 Setting the chamber address ............................................................................................................ 51
12.7 Display brightness ............................................................................................................................. 51
13. TOLERANCE RANGE SETTINGS ....................................................................... 52
13.1 Setting the delay time for tolerance range alarm .............................................................................. 52
13.2 Setting the temperature tolerance range .......................................................................................... 52
13.3 Setting the CO2 tolerance range ....................................................................................................... 53
13.4 Setting the delay time for door open alarm ....................................................................................... 53
14. CHAMBER SETTINGS (FOR EXPERIENCED USERS ONLY) ........................... 54
14.1 Setting the humidity control ............................................................................................................... 54
14.2 Setting the door heating offset .......................................................................................................... 54
14.3 Adjusting the heating power – for BINDER Service only .................................................................. 55
15. NOTIFICATION AND ALARM FUNCTIONS ........................................................ 55
15.1 Alarm messages ............................................................................................................................... 55
15.2 Information messages ....................................................................................................................... 57
15.3 Activating / deactivating the audible alarm (alarm buzzer) ............................................................... 58
15.4 Measures in case of alarm ................................................................................................................ 58
15.4.1 Door open al arm ..................................................................................................................... 58
15.4.2 Safety controller temperature alarm ....................................................................................... 58
15.4.3 Temperature tolerance range alarm (overtemperature / too low temperature) ...................... 59
15.4.4 CO2 tolerance range alarm (CO2 over/under concentration) .................................................. 60
15.4.5 CO2 pressure alarm ................................................................................................................ 60
15.4.6 Power failure alarm ................................................................................................................. 61
15.4.7 Alarms referring to temperature sensor failures ..................................................................... 62
15.4.8 Alarms referring to CO2 sensor failure .................................................................................... 63
15.4.9 Alarm on ineffective sterilization ............................................................................................. 63
15.5 Zero-voltage relay alarm output ........................................................................................................ 64
CB-S / CB-S-UL (E7) 06/2020 page 3/119
16. ETHERNET NETWORK SETTINGS .................................................................... 65
16.1 Showing the network settings ........................................................................................................... 65
16.1.1 Showing the chamber‘s MAC address .................................................................................... 65
16.1.2 Showing the IP address .......................................................................................................... 65
16.1.3 Showing the subnet mask ....................................................................................................... 66
16.1.4 Showing the standard gateway ............................................................................................... 66
16.1.5 Showing the DNS server address ........................................................................................... 66
16.1.6 Showing the DNS chamber name .......................................................................................... 67
16.2 Changing the configuration of the network settings .......................................................................... 67
16.2.1 Selecting the type of IP address assignment (automatic / manual) ....................................... 67
16.2.2 Selecting the type of assignment of the DNS server address (automatic / manual) .............. 68
16.2.3 Assigning the IP address ........................................................................................................ 68
16.2.4 Setting the subnet mask ......................................................................................................... 69
16.2.5 Setting the standard gateway ................................................................................................. 69
16.2.6 Assigning the DNS server address ......................................................................................... 70
17. DATA RECORDER .............................................................................................. 70
17.1 Recorded data ................................................................................................................................... 70
17.2 Storage capacity ............................................................................................................................... 71
17.3 Setting the storage rate for the “DL1” recorder data ......................................................................... 71
17.4 Deleting the data recorder................................................................................................................. 71
18. USB MENU: D ATA TR ANSFER VIA USB INTERFACE ..................................... 72
18.1 Connecting the USB stick ................................................................................................................. 72
18.2 Import function .................................................................................................................................. 73
18.3 Export functions ................................................................................................................................ 73
18.4 Ongoing data transfer ....................................................................................................................... 74
18.5 Error during data transmission .......................................................................................................... 74
18.6 Removing the USB stick ................................................................................................................... 74
19. REFERENCE MEASUREMENTS ........................................................................ 75
19.1 CO2 reference measuring ................................................................................................................. 75
19.1.1 Measuring the CO2 concentration indirectly via the pH of the cell medium ............................ 75
19.1.2 Measuring CO2 directly via chemical indicator tubes.............................................................. 76
19.1.3 Measuring CO2 directly with an electronic inf rar ed m eas uring de vice ................................... 77
19.2 Temperature reference measurement .............................................................................................. 77
20. OPTIONS .............................................................................................................. 77
20.1 Silicone access ports 30 mm / 1.18 in, closable with 2 silicone plugs (8012-0558 rear, 8012-0559
left, 8012-0560 right) (option) ............................................................................................................ 77
20.2 Base on castors (option) ................................................................................................................... 78
20.3 Flat stacking frame (option)............................................................................................................... 78
20.4 APT-COM™ 4 Multi Management Software (option) ........................................................................ 78
20.5 Data logger kit (option) ...................................................................................................................... 78
20.6 Analog outputs for temperature and CO2 (option) ............................................................................ 79
21. AVOIDING MICROBIAL CONTAMINATION........................................................ 79
21.1 Cells and media ................................................................................................................................ 79
21.2 Laboratory conditions / equipment around the CO2 incubator .......................................................... 79
21.3 Working and behavior in the lab ....................................................................................................... 80
21.4 Chamber design and equipment ....................................................................................................... 80
21.5 Handling the CO2 incubator .............................................................................................................. 81
22. CLEANING, DECONT AMI NATION / DISINFECTION, AND STERILIZATION .... 82
22.1 Cleaning ............................................................................................................................................ 83
22.2 Decontamination / chemical disinfection of the chamber .................................................................. 84
22.3 Disinfection of the CO2 sensor .......................................................................................................... 85
CB-S / CB-S-UL (E7) 06/2020 page 4/119
23. HOT-AIR STERILIZATION AT 180 °C / 356 °F .................................................... 86
23.1 Overview ........................................................................................................................................... 86
23.2 Performing a hot-air sterilization ....................................................................................................... 87
23.2.1 Preparations for hot-air sterilization ........................................................................................ 87
23.3 Starting and running the hot-air sterilization cycle ............................................................................ 88
23.3.1 Starting sterilization ................................................................................................................. 88
23.3.2 Performance during sterilization ............................................................................................. 89
23.3.3 Completing the entire sterilization cycle ................................................................................. 89
23.4 Prematurely terminating the sterilization cycle – effects ................................................................... 90
23.4.1 Premature termination after less than 6 hours: Ineffective sterilization .................................. 90
23.4.2 Premature termination after more than 6 hours, i.e., during the cooling-do wn phas e:
successful sterilization ............................................................................................................ 91
23.5 Prematurely terminating the sterilization cycle – procedure ............................................................. 91
23.5.1 Cancelling sterilization via the controller menu ...................................................................... 91
23.5.2 Opening the outer door ........................................................................................................... 92
23.5.3 Turning off the chamber .......................................................................................................... 92
24. MAINTEN ANCE, AND SERVICE, TROUBLESHOOTING, REPAIR, TESTING .. 93
24.1 General information, personnel qualification..................................................................................... 93
24.2 Maintenance intervals, service .......................................................................................................... 94
24.3 Service Reminder .............................................................................................................................. 94
24.4 Gas inlet fine filter ............................................................................................................................. 95
24.5 Simple troubleshooting ...................................................................................................................... 95
24.5.1 General ................................................................................................................................... 95
24.5.2 Temperature ........................................................................................................................... 96
24.5.3 CO2 ......................................................................................................................................... 97
24.5.4 Humidity .................................................................................................................................. 98
24.5.5 Controller ................................................................................................................................ 98
24.5.6 Sterilization ............................................................................................................................. 98
24.6 Sending the chamber back to BINDER GmbH ................................................................................. 99
25. DISPOSAL.......................................................................................................... 100
25.1 Disposal of the transport packing .................................................................................................... 100
25.1.1 Outer chamber packing......................................................................................................... 100
25.1.2 Packing inside the chamber, equipment ............................................................................... 100
25.2 Decommissioning ............................................................................................................................ 101
25.3 Disposal of the chamber in the Federal Republic of Germany ....................................................... 101
25.4 Disposal of the chamber in the member states of the EU except for the Federal Republic of
Germany................................................................
25.5 Disposal of the chamber in non-member states of the EU ............................................................. 103
.......................................................................... 102
26. TECHNICAL DESCRIPTION .............................................................................. 104
26.1 Factory calibration and adjustment ................................................................................................. 104
26.2 Over current protection ................................................................................................................... 104
26.3 Definition of usable volume ............................................................................................................. 104
26.4 CB-S / CB-S-UL technical data ....................................................................................................... 105
26.5 Important conversion data for non-SI units ..................................................................................... 106
26.6 Conversion table for gas inlet pressures, bar – psi ......................................................................... 107
26.7 Equipment and options (extract) ..................................................................................................... 107
26.8 Options, accessories, and spare parts (extract) ............................................................................. 108
26.9 Dimensions...................................................................................................................................... 109
27. CERTIFICATES AND DECLARATIONS OF CONFORMITY ............................. 111
27.1 EU Declaration of Conformity.......................................................................................................... 111
28. PRODUCT REGIS TRATION .............................................................................. 113
29. CONTAMIN ATION CLEARANCE CERTIFICATE ............................................. 114
29.1 For chambers located outside the USA and Canada ..................................................................... 114
29.2 For chambers in the USA and Canada ........................................................................................... 117
CB-S / CB-S-UL (E7) 06/2020 page 5/119
Carefully read the com plete o perat ing i nstruc tions of t he cham ber prior t o inst alli ng and
Dear Customer,
For the correct operation of the C O
manual completely and carefully and observe all instructions as indicated. Failure to read, understand
and follow the instruc tions may result in personal inj ury. It c a n a ls o lead to damage to the chamber and/or
poor equipment performance.
incubator CB-S / CB-S-UL, it is important that you read this op erating
2
1. Safety
1.1 Personnel Qualification
The chamber m ust only be installed, tested, and start ed up by personn el qualified f or assembly, startup,
and operation of the chamber. Qualified personnel are persons whose professional education,
knowledge, experi ence and knowledge of rel e vant s ta n dar ds a llo w th em to assess, carr y out , and i den tify
any potential hazards i n the work assigned to them. T hey must hav e been tra ined and instructed, a nd be
authorized, to work on the chamber .
The chamber should only be operated by laboratory personnel especially trained for this purpose and
familiar with all prec aution ar y measures requir ed for work ing in a laboratory. Observe the nationa l reg ulations on minimum age of laboratory personnel.
1.2 Operating manual
This oper ating manual is part of t he components of deliver y. Always keep it hand y for reference in the
vicinity of the chamber. If selling the unit, hand over the operating manual to the purchaser.
To avoid injuries and damage observe th e safety instructions of the operating m anual. Failure to follow
instructions and safety precautions can lead to significant risks.
DANGER
Dangers due to failure to observe the ins truc tions and safety precautions.
Serious injuries and chamber damage. Risk of death.
Observe the safety instructions in this Operating Manual.
Follow the operating procedures in this Operating Manual.
using the chamber.
Keep the operating manual for future reference
Make sure that all persons who use the chamber and its associated work equipment have
read and understood the Operating Manual.
This Operating Manu al is supplem ented and updated as needed. Al ways use the m ost recent version of
the Operating Manual. When in doubt, call the BINDER Service Hotline for information on the up-todateness and validity of this Operating Manual.
1.3 Legal considerations
This operating m anual is for informationa l purposes only. It co ntains information f or correct and safe installing, start-up, operat ion, decommissioning, cleani ng and maintenance of the product. No te: the contents and the product described are subject to change without notice.
Understanding and obs erving the instructions in this operat ing manual are prerequisites for ha zard-free
use and safety dur ing oper ation and m ainten ance. Im ages are to pro vide basic under stan ding. The y ma y
deviate from the actual ver sion of the cham ber. The act ual scope of deliver y can, due to opt ional or special design, or due to recent technical changes, deviate from the information and illustrat ions in these
instructions this operating manual.
CB-S / CB-S-UL (E7) 06/2020 page 6/119
In no event shall BINDER be held liable f or any damages , direct or incidental ar ising out of or related to
the use of this manual.
This operating manual ca nnot cover all conc eivable applicati ons. If you would like additional inf ormation,
or if special problem s arise that are not suff iciently addressed in th is manual, please ask your dealer or
contact us directly, e.g. by phone at the number located on page one of this manual
Furthermore, we emphas ize that the contents of this operating manual are not p art of an earlier or existing agreement, des c ripti on, or leg al rel ati ons h ip, nor d o t h e y m od ify such a relationship. All o bl ig ati ons on
the part of BINDER deriv e from the res pective purchase contr act, which also con tains the entire and exclusively valid stat ement of warrant y administration a nd the genera l terms and co nditions, as wel l as the
legal regulations va lid at the time the contr act is concluded. The s tatements in this m anual neither augment nor restrict the contractual warranty provisions.
1.4 Structure of the safety instructions
In this operating manual, the following safety definitions and symbols indicate dangerous situations in
accordance with the standards ISO 3864-2 and ANSI Z535.6.
1.4.1 Signal word panel
Depending on the s eriousness and pro bability of serio us consequences, potential dangers are identified
with a signal word, the corresponding safety color, and if appropriate, the safety alert symbol.
DANGER
Indicates an imminently hazardous situation that, if not avoided, will result in death or serious
(irreversible) injury.
WARNING
Indicates a potentially hazardous situation which, if not avoided, could result in death or serious
(irreversible) injury
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor
(reversible) injury
NOTICE
Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product
and/or its functions or of a property in its proximity.
1.4.2 Safety alert symbol
Use of the safety alert symbol indicates a risk of injury.
Observe all measures that are marked with the safety alert symbol in order to avoid death or
injury.
CB-S / CB-S-UL (E7) 06/2020 page 7/119
Warning signs
Electrical hazard
Hot surface
Explosive Atmosphere
poisoning hazard
or chemical burns
Mandatory action signs
instructions
Environment protection
Wear protective gloves
Wear safety goggles
Prohibition signs
water
1.4.3 Pictograms
Lifting hazard
Harmful substances
Mandatory regulation
Gas cylinders
Biohazard
Read operating
CO
suffocation and
2
Risk of corrosion and /
Disconnect the power
plug
Stability hazard
Pollution Hazard
Lift with several persons
Do NOT touch
Do NOT spray with
Do NOT climb
Information to be observed in order to ensure optimum function of the product.
1.4.4 Word message panel structure
Type / cause of hazard.
Possible consequences.
∅ Instruction on how to avoid the hazard: prohibition
Instruction on how to avoid the hazard: mandatory action
Observe all other n otes and inform ation not necessarily em phasized in the sam e way, in order to avoid
disruptions that could result in direct or indirect injury or property damage.
CB-S / CB-S-UL (E7) 06/2020 page 8/119
Pictograms (Warning signs)
Service label
Figure 1: Position of labels
Figure 2: Position of labels
1.5 Localization / position of safety labels at the chamber
The following labels are located on the chamber:
Hot surface
Risk of injury (UL chambers only).
Observe the safety instructions in the
operating manual.
on the CO2 incubator CB-S
on the CO2 incubator CB-S-UL
Keep safety labels complete and legible.
Replace safety labels that are no longer legible. Contact BINDER Service for these replacements.
CB-S / CB-S-UL (E7) 06/2020 page 9/119
Indications of the type plate
(example)
Information
BINDER
Manufacturer: BINDER GmbH
CB-S 170
Model designation
CO2 Incubator
Device name: CO2 Incubator
Serial No.
000000000000
Serial No. of the chamber
Built
2020
Year of construction
190 °C
374 °F
IP protection
20
Type of IP protection acc. to standard EN 60529
Temp. safety device
DIN 12880
Temperature safety device acc. to standard DIN 12880:2007
Class
3.1
Class of temperature safety device
Art. No.
9040-0189
Art. No. of the chamber
Project No.
---
Optional: Special application acc. to project no.
1,30 kW
Nominal power
6,1 A
Nominal current
200-230 V / 50 Hz
200-230 V / 60 Hz
1 N ~
Current type
Nominal temp.
190 °C
1,30 kW / 6,1 A
374 °F
200-230 V / 50 Hz
IP protection
20
200-230 V / 60 Hz
Safety device
DIN 12880
1 N ~
Class
3.1
Art. No.
9040-0189
Project No.
Built
2020 CO2 Incubator
BINDER GmbH
www.binder-world.com
CB-S 170
E7
Serial No. 00000000000000
1.6 Type plate
Position of type plate: left chamber side (seen from front), at the bottom in the middle.
Figure 3: Position of type plate
Figure 4: Type plate (example CB-S 170 standard chamber, 9040-0189)
Nominal temperature
Im Mittleren Ösch 5
78532 Tuttlingen / Germany
Nominal temperature
Made in Germany
CB-S / CB-S-UL (E7) 06/2020 page 10/119
Nominal voltage range +/- 10%
at the indicated power frequency
Symbol on the type plate
Information
CE conformity marking
Electrical and electronic equipment manufactured / placed on
the market in the EU after 13 August 2005 and to be disposed of
in a separate collection according to Directive 2012/19/EU on
waste electrical and electronic equipment (WEEE).
1.7 General safety instructions on installing and operating the chamber
With regard to operat ing the chamber and to the installation location, please o bserve the local and national regulations rel evant for your country (for Germ any: DGUV guidelines 213-850 on saf e working in
laboratories, issued by the employers’ liability insurance association).
BINDER GmbH is only respons ible for the safety feat ures of the chamber provided s killed electricians or
qualified personnel authorized by BIND ER perform all m aintenance and rep air, and if com ponents relating to chamber safety are replaced in the event of failure with original spare parts.
To operate the chamber, use only origin al BINDER ac cessories or accessories from thir d-party suppli ers
authorized by BINDER. The user is responsible for any risk caused by using unauthorized accessories.
NOTICE
Danger of overheating due to lack of ventilation.
Damage to the chamber.
∅ Do NOT install the chamber in unventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
Observe the prescribed minimum distances when installing the chamber (chap.3.4).
Do not install or operate the chamber in ha zar dous lo c ations .
DANGER
Danger of explosion due to combustible du sts or explosive mixtures in the vicinity
of the chamber.
Serious injury or death from burns and / or explo sion pressure.
∅ Do NOT operate the chamber in potentially explosive areas.
KEEP combustible dust or air-solvent mixtures AWAY from the chamber.
The chamber does not dispose of any measures of explosion protection.
DANGER
Danger of explosion due to introduction of flammable or explosive substances in
the chamber.
Serious injury or death from burns and / or explosion pressure.
∅ Do NOT introduce any substance into the chamber which is combustible or explosive at
working temperature.
∅ Do NOT introduce any combustible dust or air-solvent mixture in the inner chamber.
Any solvent contai ned in th e charging m aterial must not b e explosi ve or inflam mable. I.e., irrespecti ve of
the solvent concentrati on in the steam room, NO explos ive mixture with air m ust form. The temperature
inside the cham ber m ust lie belo w the f lash point or b elow the s ublim ation po int of the chargi ng m aterial.
Familiarize yourself with the physical and chemical properties of the charging material, as well as the
contained moisture constituent and its behavior with the addition of heat energy and humidity.
CB-S / CB-S-UL (E7) 06/2020 page 11/119
Familiarize yourself with any potential health risk s caused by the ch arging material, the contained m oisture constituent or b y reaction products that m ay arise during the temperature process. Take adequate
measures to exclude such risks prior to putting the CO
incubator into operation.
2
DANGER
Electrical hazard by water entering the chamber.
Deadly electric shock.
∅ The chamber must NOT become wet during operation, cleaning, or maintenance.
∅ Do NOT install the chamber in damp areas or in puddles.
Set up the chamber in a splash-proof manner.
The chambers were produced in accord ance with VDE regulations and were routinely tested in acc ordance to VDE 0411-1 (IEC 61010-1).
During and after a sterilization the temperature of the inner surfaces almost equals the set-point. The
glass door, the glass door handle, and the inner chamber will become hot during a sterilization.
CAUTION
Danger of burning by touching hot chamber parts during or after a sterilization.
Burns.
∅ Do NOT touch the glass door, the glass door handle, the inner surfaces, and door gas-
kets during or after a sterilization.
Danger of injury and damages by the chamber tipping over.
Injuries and damage to the chamber and the charging material
∅ Do NOT load the chamber door with heavy objects while it is open.
WARNING
CB-S / CB-S-UL (E7) 06/2020 page 12/119
Always close the valve ev e n with app ar ent l y empty cylinders; screw on the c ap when not in
1.8 Precautions when working with CO2 gas
Carbon dioxide (CO2) in high conce ntrations is hazardous to he alth. It is colorless and almost odorless
and therefore practica lly impercepti ble. Vent out an y CO
or a suitable connection to an exhaust system. We recommend installing a CO
gas that m ay escape via goo d room ventilat ion
2
warning system.
2
DANGER
Danger of suffocation and poisoning by high concentration of CO2 (> 4 Vol.-%).
Death by suffocation.
∅ Do NOT set up chambers in non-ventilated recesses.
Ensure technical ventilation measures.
Observe the relevant regulations for handling CO
Close the CO
supply when decommissioning the chamber.
2
1.9 Precautions when handling gas cylinders
General information for safe handling of gas cylinders:
• Store and use gas cylinders only in well-ventilated locatio ns .
.
2
• Open the gas cylinder valve slowly to avoid pressure surges
• Secure gas cylinders during storage and use against falling (chaining).
• Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them.
•
use. Return gas cylinders with the valve closed.
• Do not open gas cylinders by force. Mark them when dam aged
• Protect gas cylinders against fire, e.g. do not store together with flammable liquids
• Observe relevant regulations for dealing with gas cylinders.
Secure the gas cylinders against falling and other mechanical damage.
WARNING
Risk of injury through sudden release of the stored pressure energy when the valve
safety is torn off.
Injuries.
Secure gas cylinders against falling (chaining).
Transport gas cylinders with a cylinder cart.
The valve of the gas cylinder always must be closed before screwing on or unscrewing the gas hose.
WARNING
Risk of injury through sudden release of the stored pressure energy when opening
the cylinder valve of a not connected cylinder.
Injuries.
Close the gas cylinder valve before connecti ng or remov ing the gas hose.
After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or
diluted soap solution).
CB-S / CB-S-UL (E7) 06/2020 page 13/119
1.10 I nte nded use
Observing the instructions in this operating manual and conducting regular maintenance
work (chap. 24) is part of the intended use.
Any use of the chambers that does not comply with the requirements specified in this Operating
Manual shall be considered improper use.
Other applications than those described in this chapter are not approved.
Use
incubators CB-S / CB-S-UL are suitable f or the cultivation of m ammal cells under typical con ditions
CO
2
of approx. 37 °C / 98. 6 °F . The chambers permit s ettin g def ined pH con ditions b y comm on NaHC O
er systems of c ommercial cell media b y k eep in g a n exact CO
atmosphere i ns ide . T he c hambers guaran-
2
tee high humidity inside to avoid osmolarity increasing caused by the evaporation of the cell media.
The chambers are suitable for exact conditioning of harmless materials.
Requirements for the chamber load
Any solvent any solvent must not be explosive and flammable. Components of the charging material must
NOT form an explosive m ixture with air. The operat ing temperature m ust lie below the flash point or below the sublimation point of the c harging m aterial . An y component of the charging material m ust NO T be
able to release toxic gases.
buff-
3
The charging mater ial shall not conta in any corrosi ve ingredients that may damage th e machine com ponents made of stainless steel, aluminum, and copper. Such ingredients include in particular acids and
halides. Any corrosive damage caused by such ingredients is excluded from liability by BINDER GmbH.
The chamber does not dispose of any measures of explosion protection.
DANGER
Explosion or implosion hazard and danger of poisoning through the introduction of
unsuitable loading material.
Poisoning. Serious injury or death from burns and / or explosion pressure.
∅ Do NOT introduce any substance combustible or explosive at working temperature into
the chamber, in particular no energy sources such as batteries or lithium-ion batteries.
∅ NO explosive dust or air-solvent mixture in the inner chamber.
∅ Do NOT introduce any substance which could lead to release of toxic gases.
Contamination of the chamber by toxic, infectious or radioactive substances must be prevented
WARNING
Danger of intoxication and infection through contamination of the chamber with
toxic, infectious or radioactive substances.
Damages to health.
Protect the interior of the chamber from contamination by toxic, infectious or radioactive
substances.
Take suitable protective measures when introducing and removing toxic, infectious or
radioactive material
In case of foreseeable use of the device there is no risk for the user through the integrat ion of the cham ber into systems or by special environm ental or operating conditions in the sense of EN 61010-1:2010.
For this, the intended use of the chamber and all its connections must be observed.
CB-S / CB-S-UL (E7) 06/2020 page 14/119
Medical devices
The chambers are not classified as medical devices as defined by the Medical Device Directive
93/42/EEC.
Due to the special demands of the Medical Device Directive (MDD), these chambers are not
qualified for sterilization of medical devices as defined by the directive 93/42/EWG.
Personnel Requirements
Only trained person nel with knowledge of the Oper ating Ma nual can s et up and install the c ham ber, start
it up, operate, clea n, and take it out of operat ion. Service and repairs call f or further technical requirements (e.g. electrical know-how), as well as knowledge of the service manual.
Installation site requirements
The chambers are designed for setting up inside a building (indoor use).
The requirements des cribed in the Operating Manu al for installation s ite and ambient conditions (C hap.
3.4) must be met.
WARNING: If customer should use a BINDER chamber running in non-supervised continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen or
samples to split such specimen or samples and store them in at least two chambers, if this is
feasible.
Relevant regulations for dealing with CO2 and gas cylinders must be observed.
1.11 Foreseeable Misuse
Other applications than those described in chap. 1.10 are not approved.
This expressly incl udes the following misuses ( the list is not exhaustive), which pos e risks despite the
inherently safe construction and existing technical safety equipment:
• Non-observance of Operating Manual
• Non-observanc e of inf ormation and warnings on th e chamber (e.g. co ntro l uni t messages, safet y ide n-
tifiers, warning signals)
• Installation , startup, operation, maintenance and re pair by untrained, insufficiently qualified, or unau-
thorized personnel
• Missed or delayed maintenance and testing
• Non-observance of traces of wear and tear
• Insertion of materials excluded or not permitted by this Operating Manual.
• Non-compliance with the admissible parameters for processing the respective material.
• Failure to comply with the relevant regulations for handling gas cylinders
• Failure to comply with the relevant regulations for handling CO
2
• Operation of the chamber without ventilation measures
• Installation, testing, service or repair in the presence of solvents
• Installation of replacement parts and use of accessories and operating r esources not specified and
authorized by the manufac turer
• Installation , startup, operat ion, maintenance or repair of the c hamber in absence of operating instr uc-
tions
• Bypassing or c hang in g pr ot ec tive systems, operation of the c hamber without the designated prot ect iv e
systems
CB-S / CB-S-UL (E7) 06/2020 page 15/119
• Non-observance of messages regarding cleaning and disinfection of the chamber.
• Spilling water or clean ing agent on the cham ber, water penetrating into the c hamber dur ing operat ion,
cleaning or maintenance.
• Cleaning activity while chamber is turned on.
• Operation of the chamber with a damaged housing or damaged power cord.
• Continued operation of the chamber during an obvious malfunction
• Insertion of objects, particularly metallic objects, in louvers or other openings or slots on the chamber
• Human error (e.g. insufficient experience, qualification, stress, exhaustion, laziness)
To prevent these and ot her r isk s from incorrect operation, it is rec om mended the operator issue o per at ing
instructions and standard operating procedures (SOPs).
1.12 Residual Risks
The unavoidable design f eatures of a chamber, as well as its proper f ield of application, can also pose
risks, even during corr ect operation. These residual risk s include hazards which, despite the in herently
safe design, existing technical protective equipment, safety precautions and supplementary protective
measures, cannot be ruled out.
Messages on the chamber and in the Operating Manual warn of residual risks. The consequences of
these residual risk s and the m easures required to prevent them ar e listed in th e Operati ng Manu al. Mor eover, the operator must take measures to minimize hazards from unavoidable residual risks. This includes, in particular, issuing oper ati ng instr uc tions .
The following list s ummarizes the hazar ds against which this Operating Manua l and the Service Manual
warn, and specifies protective measures at the appropriate spots:
Unpacking, Transport, Installati on
• Sliding or tilting the chamber
• Setup of the chamber in unauthorized areas
• Installation of a damaged chamber
• Installation of a chamber with damaged power cord
• Inappropriate site of installation
• Missing protective conductor connection
Normal operation
• Assem bly errors
• Contact with hot surfaces on the housing
• Contact with hot surfaces in the interior and inside of doors
• Emission of non-ionizing radiation from electrical operating resources
• Contact with live parts in normal state
Cleaning and Decontamination
• Penetration of water into the chamber
• Inappropriate cleaning and decontamination agents
• Enclosure of persons in the interior
CB-S / CB-S-UL (E7) 06/2020 page 16/119
Malfunction and Damage
• Continued oper ation of the chamber during an obvio us malfunction or outage of the heating or gas
systems
• Contact with live parts during error status
• Operation of a unit with damaged power cord
Maintenance
• Maintenance work on live parts.
• Execution of maintenance work by untrained/insufficiently qualified personnel
• Electrical safety analysis during annual maintenance not performed
Trouble-shooting and Repairs
• Non-observance of warning messages in the Service Manual
• Trouble-shooting of live parts without specified safety measures
• Absence of a plausibility check to rule out erroneous inscription of electrical components
• Performance of repair work by untrained/insufficiently qualified personnel
• Inappropriate repairs which do not meet the quality standard specified by BINDER
• Use of replacement parts other than BINDER original replacement parts
• Electrical safety analysis not performed after repairs
1.13 O perating instructions
Depending on the appl ication and location of the chamber , it is recommended that the operator of the
chamber provides the re lev ant inf orm ation f or saf e op eratio n of the c ham ber in a s et of oper ating instr uctions.
Keep these operating instructions with the chamber at all times in a place where they are
clearly visible. They must be comprehensible and written in the language of the employees.
CB-S / CB-S-UL (E7) 06/2020 page 17/119
1.14 Measures to prevent accidents
The manufacturer took the following measures to prevent dangers:
• Indications on the type plate
See operating manual chap. 1.6.
• Operating manual
An operating manual is available for each chamber.
• Overtemperature monitoring
The chamber is equipped with a temperature display, which can be read from outside.
The chamber is equipped with an additional saf et y controller (tem peratur e saf ety device c lass 3.1 ac c.
to DIN 12880:2007). Visual and audible (buzzer) signals indicate temperature exceeding.
• Safety, measurement, and control equipment
The safety, measuring, and control equipment is easily accessible.
• Electrostatic ch arg e
The interior parts are grounded.
• Non-ionizing radiation
Non-ionizing radiatio n is not intentionall y produced, but release d only for technical reas ons by electrical equipment (e.g. power cables). T he machine is equipped with no per manent magnets. If persons
with active implants ( e.g. pacemaker s, defibrillators) keep a safe distance (distan ce of field source to
implant) of 30 cm, an influence of these implants can be excluded with high probability.
• Protection against touchable surfaces
Tested according to EN ISO 13732-1:2008.
• Floors
See operating manual chap. 3.4 for correct installation
• Cleaning
See operating manual chap. 22.1.
CB-S / CB-S-UL (E7) 06/2020 page 18/119
2. Chamber description
The CO2 incubators CB-S / CB-S-UL series were produc ed with great care using the latest tools for development and produc ti on. They can be oper at ed in a t emperature range from 6 °C / 10.8 °F ab ove ambient temperature up to +50 °C / 122°F and a CO
equipped with a m icroprocessor contr oller for tem perature and CO
to one-tenth of a degree resp. 0.1 vol.-%. T he y are availab le in dif f erent voltages .
Material: The inner cham ber, the pre-h eating cham ber and the ins ide of the d oors are all made of s tainless steel V2A (German materia l no. 1.4301, US equivalent AISI 304). The inner surfaces are smooth and
therefore easy to clean. T h e inner cham ber is deep-drawn from one p iece, p olis hed (s uitab le f or pharm aceutical applications) and has no welds or inaccessible corners. The hinges and the seal of the inner
glass door are glue d from the outside to aid cl eaning of the inner c ham ber. When op erating the cham ber
at high temperatures (sterilization), the im pact of the oxygen in the air may cause discoloration of the
metallic surfaces (yellowish-brown or blue) b y natural oxidation processes. T hese colorations are harmless and will in no way impair the function or quality of the chamber.
The perforated she lv es are also made of s ta in les s s te el (German mater ial no . 1.4016, US equ ival ent AISI
430). You can insert a maximum of 6 (CB-S / CB-S-UL 170) bzw. 8 (CB-S / CB-S-UL 260) shelves.
The housing is RAL 7 035 powder-coated. All corners and edges are also c ompletely coate d. The standard chamber door is hinged right. The chamber is optionally available with the door hinged left.
Sterilization: The heating system of the chamber permits hot-air auto-sterilization at 190 °C / 374 °F.
Thus, a temperature of at least 180 °C / 356 °F is maintained on al l inter na l s urf ac es , r esulti ng i n ster i lization of the entire inner chamber.
range of 0 vol.-% up t o 20 vol.-%. The chamber s are
2
levels and a dig ital display accurat e
2
Safety: T hanks to the standard s afety devic e (class 3.1 accord ing to DIN 12880:2007), the set t emperature is maintained in case of failure.
An error diagnost ics system monitors the c hamber functions and ge nerates audible and visual warning
and alarm messages. The door is monitored for being closed.
The controller provid es access control by com bining password pr otection with diff erent authorizati on levels.
system: A highly pre cise, drift-free CO2 infrared measuring s ystem in combination with the p erma-
CO
2
nent mixture of CO
precise and constant CO
gas through a special proprietary gas mixing head developed by BINDER allows
2
concentrations for long per iods. This creates optimum growth conditions for
2
cultures. The gas ent er s the c hamber via a fine filter (aseptic f ilt er ) with a h ig h f ilt r ation ef f iciency that also
filters the smallest particles.
The CO
sensor can be re m oved fr om the inner ch amber by hand and cleaned with s uita ble d etergent s if
2
needed.
Fast reaction times , maximum accurac y and selectivity charac terize the CO
chamber. The accurac y of the CO
measuring s ystem is based on an infrar ed measuring cell with NDIR
2
m easuring procedure of the
2
(non-dispersive inf rared) sensor, which continuously regul ates to a reference value. Therefore, distur bance variables an d aging phenom ena in th e meas uring s ystem are almost completel y elim inated, so that
this measuring system , in contrast to other measuring procedures , remains practic ally drift-free bet ween
calibrations and is entirely selective for CO
The CO
depends on the num ber of CO
measuring cell contains a measuring section inside, in which the absorption of infrared light
2
molecules in the be am path. This number of CO2 molecules changes with
2
.
2
the ambient press ure in relation t o a constant volume. The d istances between the molecules are consequently pressure-dependen t. T he collisio n frequenc y of the IR-beam with CO
molecules incr eases there-
2
fore by increasing pressure . For this reason, the am bient pr essure m ust be com pensate d in or der to cor rect the displa y readin g of t he CO
concentratio n i n vol .-%. This is achieved b y en tering the altitude of the
2
site above the sea (chap. 6.4).
Controller: T he efficient RD4 chamber controller is equipped with a m ultitude of operating functions, in
addition to recorder an d a la rm functions. Set-point entr y is easil y accom plished accurate to one-tenth of a
degree resp. 0.1 vol.-% directly via t he chamber contr oller and is also poss ible directly with a c omputer
via Intranet in connecti on with the APT-COM™ 4 Mu lti Management Software (option, chap. 20.4). The
controller provides password protection for the setting menus. An error diagnostics system generates
audible and visual alarm messages.
CB-S / CB-S-UL (E7) 06/2020 page 19/119
(2)
(3)
(1)
Data monitoring and recording: The chamber is regularly equipped with a zero-voltage relay alarm
output (chap. 15.5) and optionally with analog outputs (chap. 20.6) for integration into customer systems.
The chamber is regularly equipped with an Ethernet interface for computer communication, enabling
monitoring via a netw ork. The BINDER APT-COM™ 4 Multi Managem ent Software (option, chap. 20.4)
permits network ing of up t o 100 ch am bers and con nection to a c om puter, as well as r ecordin g and re presenting temperature and CO
data.
2
A dat a logger independ ent from the cham ber controller (optio n, chap. 20.5) s erves to independentl y record the temperature values, data given out in compliance with FDA guideline 21 CFR part 11.
2.1 Chamber overview
Figure 5: CO
incubator CB-S / CB-S-UL (example CB-S 170), closed
2
(1) Triangle instrument panel w ith RD4 controller for temperature and CO
2
(2) Door handle
(3) Main power switch
CB-S / CB-S-UL (E7) 06/2020 page 20/119
(11) (10) (9) (8) (1)
(4) (5) (6)
2.2 Inner chamber
Figure 6: CO
incubator CB-S / CB-S-UL (example CB-S 170), outer door open
2
(1) Triangle instrument panel with RD4 controller for temperature and CO
(4) Glass door handle
(5) Water pan
(6) Shelves
(7) (not used)
(8) Pt 100 temperature sensor
(9) CO
(10) Gas mixing head for CO
sensor
2
2
(11) Silicone measuring port in the glass door
2
CB-S / CB-S-UL (E7) 06/2020 page 21/119
2.3 Connection panel on the rear of the chamber
(13) (14) (15) (18)
(12a) (16a) (16a) (17)
Figure 7: Rear control panel C with options
(12b) (13) (14) (15) (18)
(16b) (17)
Figure 8: Rear control panel C-UL with options
(12a) Socket for IEC connector plug / power cable 230 V AC for C
(12b) Socket for IEC connector plug / power cable 100-120 V AC for C-UL
(13) DIN-socket for zero-voltage relay alarm outputs
(14) DIN socket for analog outputs 4-20 mA (av ai lab le b y BINDER INDI VUD U AL Cus t omized Solutions)
(15) Ethernet interface for computer communication
(16a) Miniature fuse T10 A (L) 250 V AC for C
(16b) Miniature fuse T12,5 A (L) 250 V AV for C-UL
(17) Strain relief for power cable
(18) Quick acting closure socket for CO
2
CB-S / CB-S-UL (E7) 06/2020 page 22/119
2.4 Chamber doors
The outer chamber door is equipped with a heater on its inner side. The do or must be closed while t he
chamber is operating normally in order to ensure stable climatic conditions in the inner chamber.
An additional glass door enables vi ewing of the s amples witho ut disturbi ng the tem peratur e in the interior
and contaminating the samples sealing the interior of the chamber.
When the outer door is open, the CO
Delay time for the temperature and CO
After closing the outer door, the tolerance range alarm is turned off for a programmable delay
time. This prevents alarms being constantly triggered during the unstable operating phase
after opening the outer door.
2.5 Ins trum ent panel
intake valve automatically closes.
2
tolerance range alarm :
2
RD4 controller display
USB interface
Figure 9: Instrument panel with RD4 controller and USB interface
CB-S / CB-S-UL (E7) 06/2020 page 23/119
r corners with the aid of
3. Completeness of delivery, transportation, storage, and installa-
tion
3.1 Unpac king, and checking equipment and completeness of delivery
After unpacking, p lease check the chamber and its optiona l accessories, if any, based on t he delivery
receipt for completenes s and for transportation dam age. Inform the carrier immediate ly if transportation
damage has occurred.
The final tests of the manufacturer may have caused traces of the shelves on the inner surfaces. This has
no impact on the function and performance of the chamber.
Please remove an y transportati on protection devices and adh esives in/o n the chamber and o n the doors
and remove the operating manuals and accessory equipment.
For transport purpos e, a silica gel bag for dryin g purpose was added. Do not e at! Do not open the silica
gel bag and dispose of it with normal waste.
Remove any protective lamination sheet on the inner metal surfaces prior to commissioning.
CAUTION
If you need to return the chamber, please use the original packing and observe the guidelines for safe
lifting and transportation (chap. 3.2).
For disposal of the transport packing, see chap. 25.1.
Note on second-hand chambers (Ex-Demo-Units):
Second-hand chambers are chambers that were used for a short tim e for tests or exhibitions. They are
thoroughly tested before resale. BINDER ensures that the chamber is technically sound and will work
flawlessly.
Second-hand chambers are marked with a sticker on the chamber door. Please remove the sticker before
commissioning the chamber.
Risk of injury and damages by lifting heavy loads and by sliding or
tilting of the chamber due to improper lifting.
Injuries, damage to the chamber.
∅ Do NOT lift or transport the chamber using the door handle or the door.
Lift the chamber from the pallet at the four lo we
four people.
CB-S / CB-S-UL (E7) 06/2020 page 24/119
Lift the chamber at the four lower corners with the aid of 4 people and plac e it on a
ed location and lift it f rom the rolling pallet with the aid of
3.2 Guidelines for safe lifting and transportation
After operation, please observe the guidelines for temporarily decommissioning the chamber (chap. 25.2).
Empty the water pan befor e moving the incubator. In c ase of any spilling of the contents , shut down the
incubator and dry it out carefully and completely
CAUTION
Risk of injury and damages by lifting heavy loads and by sliding or tilting of the
chamber due to improper transportation.
Injuries, damage to the chamber.
Transport the chamber in its original packaging only.
For moving or shipping, secure the chamber with transport straps.
∅ Do NOT lift or transport the chamber using the door handle or the door.
rolling pallet.
Move the chamber to the d es ir
four people.
• Permissible ambient temperature range for transport: -10 °C / 14°F to +60 °C / 140°F.
You can order transport packing and rolling pallets for transportation purposes from BINDER Service.
3.3 Storage
Intermediate storage of the chamber is possible in a closed and dry room. Observe the guidelines for
temporary decommissioning (chap. 25.2).
• Permissible ambient temperature range for storage: -10 °C / 14°F to +60 °C / 140°F.
• Permissible ambient humidity: max. 70% r.h., non-condensing
When after storage in a co ld location you transf er the chamber to its warm er installation site, condensation may form in the inner chamber, on the hous ing or in the sensor com partment of the CO
ment. Before start-up, wait at least one hour until the CO
incubator h as attained ambient temperature
2
measure-
2
and is completely dry.
3.4 Location of installation and ambient conditions
Notes on the location of installation
Set up the chamber on a f lat, even surf ace, free f rom vibration and in a well-ventilated, dr y location. The
chambers are designed for setting up inside a building (indoor use).
Freestanding chambers are s uitable for installation on tables or on the optionally available st and. Note:
The site of installa tion must be capable of supportin g the chamber’s weight (see t echnical data, chap.
26.4).
Align the chamber using a spirit level to ensure even covering of the cell-cultures with the m edium. For
this purpose, manually adjust the four incubator feet.
In order to avoid contamination, never place the chamber directly on the floor.
CB-S / CB-S-UL (E7) 06/2020 page 25/119
NOTICE
Danger of overheating due to lack of ventilation.
Damage to the chamber.
∅ Do NOT install the chamber in unventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
Observe the prescribed minimum distances when installing the chamber.
Do not install or operate the chamber in potentially explosive areas.
DANGER
Danger of explosion due to combustible du sts or explosive mixtures in the vicinity
of the chamber.
Serious injury or death from burns and / or explo sion pressure.
∅ Do NOT operate the chamber in potentially explosive areas.
KEEP explosive dust or air-solvent mixtures AWAY from the vicinity of the chamber.
Ambient conditions
• Permiss ible ambient t em peratur e range f or oper ation: +18 °C / 64 .4 °F up to +30 °C / 86 °F. At elevat-
ed ambient temperature values, fluctuations in temperature can occur.
• Ideal ambien t temperature: by at l east 6 °C / 10.8 °F below the intended working temperature. E.g.,
working temperature 37 °C / 98.6 °F – resulting p ermitted ambient tem perature 31 °C / 87.8 °F and
lower
In the event of working t emperatures of less than 6 °C / 10. 8 °F above the ambient tem perature, the se t
point can be exceeded.
Do not place the chamber directly below the air outlet of an air conditioner.
The ambient temperature should not be substantially higher than the indicated ambient temperature of +22 °C +/- 3 °C / 71.6 °F ± 5.4 °F to which the specified technical data relate. For
other ambient conditions, deviations from the indicated data are possible.
Avoid direct solar radiation on the chamber.
Avoid strong drafts, e.g. by air conditioning.
• Permissible ambient humidity: 70% r.h. max., non-condensing.
• Installation he ight: max . 2000 m / 6562 ft. above sea level. After the incubator ha s been turned on f or
the first time, enter the altitude of the site above sea level into the RD4 controller (chap. 6.4).
• Wall distances: rear 100 mm / 3.94 in, sides 50 mm / 1.97 in.
To completely separate the chamber from the power supply, you must disconnect the power
plug. Install the chamber in a way that the power plug is easily accessible and can be easily
pulled in case of danger.
• Avoid any conduc tive dust in the ambiance according to the cham ber layout compl ying with pollution
degree 2 (IEC 61010-1).
CB-S / CB-S-UL (E7) 06/2020 page 26/119
Notes on handling carbon dioxide (CO
Carbon dioxide (CO
) in high conce ntrations is hazardous to he alth. It is colorless and almost odorless
2
and therefore practica lly impercepti ble. Vent out an y CO
or a suitable connection to an exhaust system. We recommend installing a CO
)
2
gas that m ay escape via goo d room ventilat ion
2
warning system.
2
DANGER
Danger of suffocation and poisoning by high concentration of CO2 (> 4 Vol.-%).
Death by suffocation.
∅ Do NOT set up chambers in non-ventilated recesses.
Ensure technical ventilation measures.
Observe the relevant regulations for handling CO
Observe the occupational exposure limit OEL for CO2 set b y the national authorities (formerl y maximum permitted work place concentration) . Check complianc e when operating a ll chambers located in the
room.
• OEL for Germany: 5000 ml/m3 (ppm) = 0,5 Vol.-%
• CO
lost with eac h door o pening: about 16.4 g , i.e. 0.0084 c ubic m eters / 0.29 6 cubic fee t (under nor-
2
mal pressure)
• CO
lost during 12h at 5 vol.-% without door opening: approx. < 2 g, i.e. 0. 001 cubic meters / 0.035
2
cubic feet (under normal pressure 1013 mbar / 14.7 psi)
An example of how to evaluate laboratory volume and air change rate:
.
2
Question: Is an air ch ange rate of 1/h suffic ient for a lab with a volum e of 100 cubic meters / 3,531.5
cubic feet with 10 CO
Calculation: CO
incubators, opened 4 times per hour?
2
concentration = CO2 lost by door opening, m ultiplied by 10 chambers, multiplied by 4
2
door openings per hour, divided by lab volume
0.0084 cubic meters x 10 x 4 div. 100 cubic meters = 0.00336, i.e. 0.336% or 3360 ppm.
0.296 cubic feet x 10 x 4 div. 3,531.5 cubic feet = 0.00336, i.e. 0.336% or 3360 ppm.
Result: The maximum permissible value of 5000 ppm is not exceeded under these operation conditions.
Even when CO
or systems operated with CO2 are handled caref ully and appropriately, a residual risk
2
remains, which can lead to life-threatening s ituations under certain circum stances . Therefore we str ongly
recommend continuo us monitoring of CO
be ensured permanently that the maximum permissible occupational exposure limit OEL for CO
% CO
for Germany) is not exceeded.
2
concentration in the am bient air of the CO2 incubator. It must
2
(0.5 vol -
2
CB-S / CB-S-UL (E7) 06/2020 page 27/119
4. Installation and connections
4.1 Shelves
You can put the she lves in differ ent positions at the line of c hannel beads in th e inner c ham ber. Hold th e
shelf straight and then insert it so it will go smoothly inside the chamber.
Permitted shelf loads:
Maximum load on one single shelf: 10 kg / 22 lb
Maximum total load on all shelves: 40 kg / 88 lb
4.2 CO2 sensor
4.2.1 Connecting the CO2 sensor
Turn off the chamber. Open the door of the inner cham ber and plug the CO2 sensor (4) into the permanently installed holding tube located in the upper part of the rear of the inner chamber.
Figure 10: Plugged-in CO
sensor (right) and gas mixing head for CO2
2
The sensor must click in correctly and sit tightly in the connection socket.
NOTICE
Danger of damage to the CO2 sensor by connecting or removing it during operation.
Damage to the CO
Connect or remove the CO
4.2.2 General notes
Connect or remove t he CO2 sensor without rotati ng and only when the incubator is turned off. Remove
the CO
and humidity from intrudin g into the m easuring c ell. It is avai lable as a spare part. Re place it whene ver it
is damaged or soiled.
sensor before r emov ing or rep lacing its filter cap. T he PTF E filter of the CO2 sensor prevents dirt
2
The accuracy of the indicated values of CO
0.08 vol.-% per 10 mbar / 0.15 psi). In order to compensate this effect when measuring the
concentration, the altitude of the installation site above sea level can be entered into the
CO
2
controller (chap. 6.4).
sensor.
2
sensor only with the chamber turned off.
2
depends on the ambient air pressure (approx.
2
CB-S / CB-S-UL (E7) 06/2020 page 28/119
NOTICE
NOTICE
NOTICE
The CO
sensor is temperature resistant up to a maxi m u m temperature of 60 °C / 140 °F.
2
Danger of damage to the CO2 sensor by excess temperature.
Damage to the CO
∅ Do NOT autoclave the CO
∅ Do NOT expose the CO
sensor.
2
Sensor.
2
sensor to hot-air sterilization..
2
The CO
sensor he ad was especiall y adjusted for the specific c hamber . To avoid confusion, an adhes ive
2
label with a ser ial num ber is adh ered to the sens or head. W hen ex changi ng the sensor, you m ust repeat
the CO
adjustment.
2
Danger of confusing CO2 sensors.
Invalid calibration.
Do NOT change the CO
Note down the serial number of the CO
sensor head.
2
sensor.
2
Avoid strong shocks when handling the CO2 sensor.
Danger of damage to the CO2 sensor by shocks.
Damage to the CO
Avoid strong shocks of the CO
sensor.
2
sensor (by putting it down hard, or dropping).
2
4.3 Water pan
The water pan permits high humidity without condensation on the inner walls of the CO2 incubator.
Place the water pan in central position on the bottom of the inner chamber. Place the wat er pan with its
narrow side centrally between the front cams on the bottom of the inner chamber.
Figure 11: Position of the water pan centr all y betwee n the front cams (arrows)
CB-S / CB-S-UL (E7) 06/2020 page 29/119
Always close the valve ev e n with app ar ent l y empty cylinders; screw on the c ap when not in
Make sure that the water pan has firm contact with the inner chamber bottom and rests tightly on it.
Fill the water pan with 1 liter of distilled, sterilized water.
We recommend c leaning (chap. 22.1) and refil ling the pans 2 to 3 times a week. F or evacuat ion, rem ove
the water pan.
We recommend using dis tilled, sterile water to achieve optim um growth results. Any corrosive dam age
that may arise following use of water of different quality or by additives is excluded from the liability
agreement.
If required, you ca n ad d micr obiologicall y inhibiti ng su bstances s uch as c opper c hips, copper s ulphate or
ethylene diamine tetra-vinegar acid (EDTA) in a concentration of
Empty the water pan bef ore moving the incub ator . In c as e of the c ont ents s p illi n g, im mediately shut down
the incubator and dry it carefully and completely.
1 to 5 mmol/l.
4.4 Gas connection
General information for safe handling of gas cylinders:
• Store and use gas cylinders only in well ventilated areas.
• Open the gas cylinder valve slowly to avoid pressure surges
• Secure gas cylinders during storage and use against falling (chaining).
• Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them
•
use. Return gas cylinders with the valve closed
• Do not open gas cylinders by force. Mark them when damaged
• Protect gas cylinders against fire, e.g. do not store together with flammable liquids
• Observe relevant regulations for dealing with gas cylinders.
Secure the gas cylinders against falling and other mechanical damage.
WARNING
Risk of injury through sudden release of the stored pressure energy when the valve
safety is torn off.
Injuries.
Secure gas cylinders against falling (chaining).
Transport gas cylinders with a cylinder cart.
The valve of the gas cylinder always must be closed before screwing on or unscrewing the gas hose.
WARNING
Risk of injury through sudden release of the stored pressure energy when opening
the cylinder valve of a not connected cylinder.
Injuries.
Close the gas cylinder valve before connecting or removing the gas hose.
After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or
diluted soap solution).
CB-S / CB-S-UL (E7) 06/2020 page 30/119
Loading...