BINDER CB 60 User Manual

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Operating Manual

APT.line™ CB (E6) CO CO

- Incubators

– Incubators with O2 control

with sterilizable NDIR sensor system for CO2 and humidity and microprocessor controller T4.12

Model Voltage Equipment Art. No.

CB 60 230 V 9040-0088, 9140-0088

CB 60-UL 100-120V 9040-0089, 9140-0089

CB 60 230 V with O2 control 9040-0090, 9140-0090

CB 60-UL 100-120V with O2 control 9040-0091, 9140-0091

CB 160 (E6) 230 V 9040-0092, 9140-0092

CB 160-UL 100-120V 9040-0093, 9140-0093

CB 160 (E6) 230 V with O2 control 9040-0094, 9140-0094

CB 160-UL 100-120V with O2 control 9040-0095, 9140-0095

CB 160 230 V with divided glass door 9040-0100, 9140-0100

CB 160-UL 100-120V with divided glass door 9040-0101, 9140-0101

CB 160 230 V with O2 control with divided glass door 9040-0102, 9140-0102

CB 160-UL 100-120V with O2 control with divided glass door 9040-0103, 9140-0103

CB 220 230 V 9040-0096, 9140-0096

CB 220-UL 100-120V 9040-0097, 9140-0097

CB 220 230 V with O2 control 9040-0098, 9140-0098

CB 220-UL 100-120V with O2 control 9040-0099, 9140-0099

BINDER GmbH

Address Post office box 102 D-78502 Tuttlingen Tel. +49 7462 2005 0 Fax +49 7462 2005 100 Internet http://www.binder-world.com E-mail info@binder-world.com Service Hotline +49 7462 2005 555 Service Fax +49 7462 2005 93 555 Service E-Mail service@binder-world.com Service Hotline USA +1 866 885 9794 or +1 631 224 4340 x3 Service Hotline Asia Pacific +852 39070500 or +852 39070503 Service Hotline Russia and CIS +7 495 98815 17

Issue 09/2013 Art. No. 7001-0229

EG – KONFORMITÄTSERKLÄRUNG

Anschrift / Address / Adresse:

Typenbezeichnung / Type / Type:

employé dans certaines limites de tension

EG – declaration of conformity

EC - DECLARATION OF CONFORMITY CE - DECLARATION DE CONFORMITE

Anbieter / Supplier / Fournisseur:

Produkt / Product / Produit:

Die oben beschriebenen Produkte sind konform mit folgenden EG-Richtlinien: The products described above are in conformity with the following EC guidelines: Les produits décrits ci-dessus sont conformes aux directives CE suivantes:

Niederspannungsrichtlinie 2006/95/EG

Low voltage directive 2006/95/EC

Directive basse tension 2006/95/CE

BINDER GmbH Im Mittleren Ösch 5, D-78532 Tuttlingen CO

Begasungsbrutschrank

Incubators

Incubateurs à CO CB 60, CB 160, CB 220

Richtlinie 2006/95/EG des Europäischen Parlaments und des Rates vom 12. Dezember 2006 zur Angleichung der Rechtsvorschriften der Mitgliedstaaten betreffend elektrische Betriebsmittel zur Verwendung innerhalb bestimmter Spannungsgrenzen

Council Directive 2006/95/EC of 12 December 2006 on the harmonization of the laws of Member States relating to electrical equipment designed for use within certain voltage limits

Directive 2006/95/CE du Parlement Européen et du Conseil du 12 décembre 2006 concernant le rapprochement des législations des États membres relatives au matériel électrique destiné à être

EMV-Richtlinie 2004/108/EG

EMC Directive 2004/108/EC

Directive CEM 2004/108/CE

Die oben beschriebenen Produkte tragen entsprechend die Kennzeichnung CE. The products described above, corresponding to this, bear the CE-mark. Les produits décrits ci-dessus, en correspondance, portent l’indication CE.

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Richtlinie 2004/108/EG des Europäischen Parlaments und des Rates vom 15. Dezember 2004 zur Angleichung der Rechtsvorschriften der Mitgliedstaaten über die elektromagnetische Verträglichkeit und zur Aufhebung der Richtlinie 89/336/EWG.

Directive 2004/108/EC of the European Parliament and of the Council of 15 December 2004 on the approximation of the laws of the Member States relating to electromagnetic compatibility and repealing Directive 98/336/EEC.

Directive 2004/108/CE du Parlement Européen et du Conseil du 15 décembre 2004 relative au rapprochement des législations des États membres concernant la compatibilité électromagnétique et abrogeant le directive 98/336/CEE.

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Sicherheit / safety / sécurité:

EMV / EMC / CEM:

Die oben beschriebenen Produkte sind konform mit folgenden harmonisierten Normen: The products described above are in conformity with the following harmonized standards: Les produits décrits ci-dessus sont conformes aux normes harmonisées suivantes:

EN 61010-1:2010 Sicherheitsbestimmungen für elektrische Mess-, Steuer-, Regel- und

Laborgeräte – Teil 1: Allgemeine Anforderungen (DIN EN 610101:2011, VDE 411-1:2011)

Safety requirements for electrical equipment for measurement, control, and laboratory use – Part 1: General requirements (IEC 61010-1:2010, BS EN 61010-1:2010)

Règles de sécurité pour appareils électriques de mesurage, de régulation et de laboratoire – Partie 1: Prescriptions générales (CEI 61010-1:2010, NF EN 61010:2011)

EN 61010-2-010:2003 Sicherheitsbestimmungen für elektrische Meß-, Steuer-, Regel- und

Laborgeräte – Teil 2-010: Besondere Anforderungen an Laborgeräte für das Erhitzen von Stoffen (DIN EN 61010-2-010:2004)

Safety requirements for electrical equipment for measurement, control, and laboratory use – Part 2-010: Particular requirements for laboratory equipment for the heating of materials (IEC 61010-2-10:2005, BS EN 61010-2-10:2003)

Règles de sécurité pour appareils électriques de mesurage, de régulation et de laboratoire – Partie 2-010 : Prescriptions particulières pour appareils de laboratoire utilisés pour l’échauffement des matières (CEI 61010-2-10:2003, NF EN 61010-2-10:2005)

EN 61326-1:2006 + Corr. 1:2008 + Corr. 2:2010

Elektrische Mess-, Steuer-, Regel- und Laborgeräte - EMVAnforderungen - Teil 1: Allgemeine Anforderungen (DIN EN 613261:2006 + Berichtigung 1:2008 + Berichtigung 2:2011)

Electrical equipment for measurement, control and laboratory use EMC requirements - Part 1: General requirements (IEC 61326-1:2005 + Corr. 1:2008 + Corr. 2:2010, BS EN 61326-1:2006+ A1:2008)

Matériel électrique de mesure, de commande et de laboratoire Exigences relatives à la CEM - Partie 1: Exigences générales (CEI 61326-1:2005 + AC1:2008, NF EN 61326-1:2006 mod.)

EN 61326-2-2:2006 Elektrische Mess-, Steuer-, Regel- und Laborgeräte – EMV-

Anforderungen. Teil 2-2: Besondere Anforderungen - Prüfanordnung, Betriebsbedingungen und Leistungsmerkmale für ortsveränderliche Prüf-, Mess- und Überwachungsgeräte in NiederspannungsStromversorgungsnetzen. (DIN EN 61326-2-2:2006)

Electrical equipment for measurement, control and laboratory use – EMC requirements. Part 2-2: Particular requirements - Test configurations, operational conditions and performance criteria for portable test, measuring and monitoring equipment used in low-voltage distribution systems. (IEC 61326-2-2:2005, BS EN 61326-2-2:2006)

Matériel électrique de mesure, de commande et de laboratoire – Exigences relatives à la CEM. Partie 2-2: Exigences particulières Configurations d’essai, conditions de fonctionnement et critères d’aptitude à la fonction des matériels portatifs d’essai, de mesure et de surveillance utilisés dans des systèmes de distribution basse tension. (CEI 61326-2-2:2005 + AC1:2007, NF EN 61326-2-2:2006)

CB (E6) 09/2013 Page 3/144

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P. M. Binder

B. Hofmann

D-78532 Tuttlingen, 20.07.2012 BINDER GmbH

Geschäftsführender Gesellschafter Managing Director Directeur général

Leiter F & E Director R & D Chef de service R&D

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Product registration

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Contents

EG – declaration of conformity ...................................................................................................................... 2

Product registration ....................................................................................................................................... 5

1. SAFETY ................................................................................................................ 10

1.1 Legal considerations ......................................................................................................................... 10

1.2 Structure of the safety instructions .................................................................................................... 10

1.2.1 Signal word panel ................................................................................................................... 10

1.2.2 Safety alert symbol ................................................................................................................. 11

1.2.3 Pictograms .............................................................................................................................. 11

1.2.4 Word message panel structure ............................................................................................... 12

1.3 Localization / position of safety labels on the unit ............................................................................. 12

1.4 Type plate.......................................................................................................................................... 14

1.5 General safety instructions on installing and operating the CO2 incubator....................................... 15

1.6 Precautions when working with gases .............................................................................................. 16

1.7 Precautions when handling gas cylinders ......................................................................................... 18

1.8 Intended use ..................................................................................................................................... 18

2. UNIT DESCRIPTION ............................................................................................ 19

2.1 Unit overview ..................................................................................................................................... 20

2.2 Instrument panel ............................................................................................................................... 20

2.3 Inner chamber ................................................................................................................................... 21

2.4 Control panel on the rear of the unit ................................................................................................. 23

3. COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND

INSTALLATION .................................................................................................... 24

3.1 Unpacking, and checking equipment and completeness of delivery ................................................ 24

3.2 Guidelines for safe lifting and transportation ..................................................................................... 24

3.3 Storage .............................................................................................................................................. 25

3.4 Location of installation and ambient conditions ................................................................................ 25

4. INSTALLATION AND CONNECTIONS ............................................................... 29

4.1 Shelves .............................................................................................................................................. 29

4.2 Permadry™ water pan ...................................................................................................................... 29

4.3 Gas connections ............................................................................................................................... 30

4.3.1 Connection of the CO2 gas cylinder ........................................................................................ 31

4.3.2 Connection of the O2 gas cylinder (unit with O2 control) ......................................................... 32

4.3.3 Connection of the N2 gas cylinder (unit with O2 control) ......................................................... 33

4.3.4 Connecting the gas hose to the unit rear (for CO2, O2, N2) .................................................... 34

4.3.5 Gas cylinder connection kits (option) ...................................................................................... 35

4.4 Electrical connection ......................................................................................................................... 36

4.5 Handling and aligning the multiple-divided inner glass door, gas proof (optional equipment) ........ 37

5. START UP ............................................................................................................ 38

5.1 Equilibration time ............................................................................................................................... 38

5.2 Factory settings ................................................................................................................................. 38

6. FUNCTIONAL OVERVIEW OF THE T4.12 CHAMBER CONTROLLER ............. 39

6.1 Menu structure .................................................................................................................................. 40

6.1.1 General menu ......................................................................................................................... 40

6.1.2 Quick menu ............................................................................................................................. 41

6.1.3 User menu .............................................................................................................................. 42

6.2 Operating modes ............................................................................................................................... 43

6.2.1 Activating the “control off” mode or change to “fixed value” operating mode ......................... 43

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Deactivating the O

6.3

6.3.1 Required gas supply of the unit with O2 control ...................................................................... 45

6.3.2 Activating / deactivating the O2 control and O2 / N2 pressure alarms ..................................... 46

6.4 Humidity control of the Permadry™ system ...................................................................................... 47

6.5 Turning on / off the interior socket voltage (with optional interior socket) ......................................... 48

6.6 Performance during and after power failure ...................................................................................... 48

6.7 Information ........................................................................................................................................ 49

control and O2/N2 pressure alarms (unit with O2 control) .................................. 45

7. SETPOINT ENTRY ............................................................................................... 50

7.1 Setting ranges ................................................................................................................................... 50

7.2 Note when setting high gas concentrations ...................................................................................... 50

7.3 Entering the setpoints via “quick menu” ............................................................................................ 52

7.4 Entering the setpoints via general menu ........................................................................................... 54

8. KEY LOCK ........................................................................................................... 56

8.1 Directly activating the key lock .......................................................................................................... 57

8.2 Automatic key lock ............................................................................................................................ 57

8.3 Changing the password for unlocking the key lock ........................................................................... 59

9. GENERAL CONTROLLER SETTINGS ................................................................ 60

9.1 Setup wizard ..................................................................................................................................... 61

9.2 Date and time settings ...................................................................................................................... 61

9.3 Selecting the menu language of the T4.12 controller ....................................................................... 63

9.4 Setting display brightness ................................................................................................................. 63

9.5 Changing the temperature unit.......................................................................................................... 64

9.6 Defining the data recording rate ........................................................................................................ 64

9.7 Factory reset ..................................................................................................................................... 65

9.8 Network configuration ....................................................................................................................... 65

9.9 Display of the network configuration ................................................................................................. 69

9.10 MAC Address .................................................................................................................................... 69

9.11 RS 422 address (with optional RS 422 interface) ............................................................................. 70

9.12 Display and entry of the device configuration – for service purpose ................................................ 71

10. DATA TRANSFER VIA USB INTERFACE........................................................... 72

10.1 Exporting data to USB drive .............................................................................................................. 72

10.2 Importing data from USB drive .......................................................................................................... 73

11. NOTIFICATIONS AND ALARMS ......................................................................... 74

11.1 Notifications overview ....................................................................................................................... 74

11.2 Alarm messages overview ................................................................................................................ 74

11.3 Alarm status ...................................................................................................................................... 76

11.4 Confirming an active “set” alarm ....................................................................................................... 77

11.5 Alarm configuration and overview ..................................................................................................... 78

11.5.1 List of active alarms ................................................................................................................ 78

11.5.2 History – list of all alarms ........................................................................................................ 79

11.5.3 Activating, deactivating, and testing the alarm buzzer ........................................................... 80

11.5.4 Activating / deactivating all alarm functions ............................................................................ 80

11.5.5 Setting the delay time after opening the door ......................................................................... 81

11.5.6 Tolerance ranges and alarm delay times................................................................................ 81

11.6 Zero-voltage relay alarm output ........................................................................................................ 86

12. EVENT LIST ......................................................................................................... 87

13. GRAPHICAL DISPLAY OF THE MEASURED VALUES ..................................... 88

13.1 Setting the sampling rate .................................................................................................................. 88

13.2 Defining the display range................................................................................................................. 89

13.3 Selecting the parameters .................................................................................................................. 90

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14. TEMPERATURE SAFETY DEVICES ................................................................... 91

14.1 Overtemperature protective device (class 1) .................................................................................... 91

14.2 Overtemperature safety controller (temperature safety device class 3.1) ........................................ 91

14.2.1 Safety controller modes .......................................................................................................... 91

14.2.2 Setting the safety controller .................................................................................................... 92

15. OPTIONS .............................................................................................................. 95

15.1 Communication software APT-COM™ 3 DataControlSystem (option) ............................................ 95

15.2 RS 422 interface (option) .................................................................................................................. 95

15.3 Silicone access ports 30 mm / 1.18 in, closable from both sides with silicon plugs (option) ............ 95

15.4 Interior socket 230V (option) ............................................................................................................. 96

15.5 Analog outputs for temperature and CO2 (option) ............................................................................ 98

15.6 Access port for extra-low voltage (option) ......................................................................................... 98

15.7 BINDER Gas Supply Service – External bottle changer for CO2, N2 or O2 (option) ......................... 99

15.8 Stands ............................................................................................................................................. 100

15.8.1 Stacking stand (option) ......................................................................................................... 100

15.8.2 Stacking adapter for direct thermal decoupled stacking (option) ......................................... 100

15.8.3 Base on castors (option) ....................................................................................................... 100

16. REFERENCE MEASUREMENTS ...................................................................... 101

16.1 CO2 reference measuring ............................................................................................................... 101

16.1.1 Measuring CO2 concentration indirectly via the pH of the cell medium ................................ 101

16.1.2 Measuring CO2 directly via chemical indicator tubes............................................................ 102

16.1.3 Measuring CO2 directly with an electronic infrared measuring device ................................. 103

16.2 Temperature reference measurement ............................................................................................ 103

17. AVOIDING MICROBIAL CONTAMINATION...................................................... 103

17.1 Cells and media .............................................................................................................................. 103

17.2 Laboratory conditions / equipment around the incubator ................................................................ 103

17.3 Working and behavior in the lab ..................................................................................................... 104

17.4 Chamber design and equipment of the CO2 incubator ................................................................... 104

17.5 Handling the CO2 incubator ............................................................................................................ 105

18. CLEANING, DECONTAMINATION / DISINFECTION, AND STERILIZATION .. 107

18.1 Cleaning .......................................................................................................................................... 107

18.2 Decontamination / chemical disinfection of the CO2 incubator ....................................................... 108

18.3 Hot-air sterilization at 180 °C / 356 °F ............................................................................................. 110

18.3.1 Overview ............................................................................................................................... 110

18.3.2 Performing a hot-air sterilization ........................................................................................... 111

18.3.3 Aborting the hot-air sterilization prematurely ........................................................................ 113

18.3.4 End of the sterilization cycle ................................................................................................. 115

19. MAINTENANCE AND SERVICE ........................................................................ 116

19.1 Maintenance intervals, service ........................................................................................................ 116

19.2 Checking the air jacket heating fan ................................................................................................. 116

19.3 Checking the humidity system fan .................................................................................................. 117

19.4 Gas inlet fine filter ........................................................................................................................... 117

19.5 Sending the unit back to BINDER GmbH ....................................................................................... 117

20. DISPOSAL.......................................................................................................... 118

20.1 Disposal of the transport packing .................................................................................................... 118

20.1.1 Outer unit packing ................................................................................................................. 118

1.1.1 Packing inside the unit and equipment ................................................................................. 118

20.2 Decommissioning ............................................................................................................................ 119

20.3 Disposal of the unit in the Federal Republic of Germany ............................................................... 119

20.4 Disposal of the unit in the member states of the EC except for the Federal Republic of Germany 120

20.5 Disposal of the unit in non-member states of the EC ..................................................................... 121

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21. TROUBLESHOOTING ....................................................................................... 122

21.1 Heating ............................................................................................................................................ 122

21.2 Gas cylinder pressure too low ......................................................................................................... 123

21.3 Gas concentration ........................................................................................................................... 125

21.4 Sterilization ...................................................................................................................................... 127

21.5 Humidity .......................................................................................................................................... 127

21.6 Controller ......................................................................................................................................... 129

21.7 Open door ....................................................................................................................................... 129

22. TECHNICAL DESCRIPTION .............................................................................. 130

22.1 Factory calibration and adjustment ................................................................................................. 130

22.2 Over current protection ................................................................................................................... 130

22.3 Definition of usable volume ............................................................................................................. 130

22.4 CB technical data ............................................................................................................................ 131

22.5 Equipment and Options ................................................................................................................... 133

22.6 Spare parts and accessories .......................................................................................................... 134

22.7 Important conversion data for non-SI units ..................................................................................... 135

22.8 Conversion table for gas inlet pressures, bar – psi ......................................................................... 135

22.9 Dimensions CB 60 .......................................................................................................................... 136

22.10 Dimensions CB 160 ........................................................................................................................ 137

22.11 Dimensions CB 220 ........................................................................................................................ 138

23. CONTAMINATION CLEARANCE CERTIFICATE ............................................. 139

23.1 For units located outside North America and Central America ....................................................... 139

23.2 For units in North America and Central America ............................................................................ 142

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Dear customer,

For the correct operation of the CO completely and carefully and observe all instructions as indicated. Failure to read, understand and follow the instructions may result in personal injury. It can also lead to damage to the unit and/or poor equipment performance

incubator CB, it is important that you read this operating manual

1. Safety

This operating manual is part of the components of delivery. Always keep it handy for reference. The device should only be operated by laboratory personnel especially trained for this purpose and familiar with all precautionary measures required for working in a laboratory. To avoid injuries and damage observe the safety instructions of the operating manual.

WARNING

Failure to observe the safety instructions. Serious injuries and unit damage.

 Observe the safety instructions in this operating manual.  Carefully read the complete operating instructions of the CO

1.1 Legal considerations

This operating manual is for informational purposes only. It contains information for installing, start-up, operation and maintenance of the product. Note: the contents and the product described are subject to change without notice.

incubator CB.

Understanding and observing the instructions in this operating manual are prerequisites for hazard-free use and safety during operation and maintenance. In no event shall BINDER be held liable for any damages, direct or incidental arising out of or related to the use of this manual.

This operating manual cannot cover all conceivable applications. If you would like additional information, or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or contact us directly by phone at the number located on page one of this manual

Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or existing agreement, description, or legal relationship, nor do they modify such a relationship. All obligations on the part of BINDER derive from the respective purchase contract, which also contains the entire and exclusively valid statement of warranty administration. The statements in this manual neither augment nor restrict the contractual warranty provisions.

1.2 Structure of the safety instructions

In this operating manual, the following safety definitions and symbols indicate dangerous situations following the harmonization of ISO 3864-2 and ANSI Z535.6.

1.2.1 Signal word panel

Depending on the probability of serious consequences, potential dangers are identified with a signal word, the corresponding safety color, and if appropriate, the safety alert symbol.

DANGER

Indicates an imminently hazardous situation that, if not avoided, will result in death or serious (irreversible) injury.

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Warning signs

Electrical hazard

Hot surface

Explosive atmosphere

poisoning hazard

or chemical burns

Biohazard

WARNING

Indicates a potentially hazardous situation which, if not avoided, could result in death or serious (irreversible) injury.

CAUTION

Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor (reversible) injury.

CAUTION

Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product and/or its functions or of a property in its proximity.

1.2.2 Safety alert symbol

Use of the safety alert symbol indicates a risk of injury. Observe all measures that are marked with the safety alert symbol in order to avoid death or

injury.

1.2.3 Pictograms

Lifting hazard

Explosive substances

Gas cylinders

Fire promoting agents

Pollution Hazard

Suffocation hazard

Harmful substances

Stability hazard

CO2 suffocation and

Risk of corrosion and /

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Mandatory action signs

plug

Environment protection

Wear protective gloves

Prohibition signs

water

Pictograms (Warning signs)

Service label

Mandatory regulation

Do NOT touch

Information to be observed in order to ensure optimum function of the product.

Read operating

instructions

Do NOT spray with

1.2.4 Word message panel structure

Type / cause of hazard. Possible consequences.

 Instruction how to avoid the hazard: prohibition  Instruction how to avoid the hazard: mandatory action

Disconnect the power

Wear safety goggles

Do NOT climb

Lift with several persons

Observe all other notes and information not necessarily emphasized in the same way, in order to avoid disruptions that could result in direct or indirect injury or property damage.

1.3 Localization / position of safety labels on the unit

The following labels are located on the unit:

Hot surface

Risk of injury

• on the outer door: CB-UL only

• above the access ports (option)

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CO2 -Incubator CB CO2 -Incubator CB-UL

Figure 1: Position of labels on the unit

Keep safety labels complete and legible.

Replace safety labels that are no longer legible. Contact BINDER service for these replacements.

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Indications of the type plate

Information

BINDER

Manufacturer: BINDER GmbH

CB 160

Model CB 160

Serial No.

00-00000

Serial No. 00-00000

Nominal temperature

187 °C 369 °F

Enclosure protection

IP 20

IP type of protection 20 acc. to EN 60529

Temp. safety device

DIN 12880

Temperature safety device acc. to standard DIN 12880

Class

3.1

Temperature safety device, class 3.1

Art. No.

9040-0092

Art. No. 9040-0092

Project No.

---

(Special application acc. to project no.)

1,30 kW

Nominal power 1,30 kW

200-240 V 1 N ~

Nominal voltage 200-240 V ± 10%, single-phase unit

5,7 A

Nominal current 5,7 Amp

50/60 Hz

Power frequency 50/60 Hz

Symbol on the type plate

Information

LABORATORY EQUIPMENT 43KM

Nominal temperature

187 °C

1,30 kW

369°F

200-240 V 1 N ~

Temp. safety device

IP 20 DIN 12880

50/60 Hz

Class

3.1

Art. No.

9040-0092

US PATS 4585923 / 5222612 / 5309981

Project No.

5405194 / 5601143 / 5773287 / 6079403

D 78532 Tuttlingen / Germany

Internet: www.binder-world.com

1.4 Type plate

Position of type plate: left unit side (seen from front), at the bottom in the middle

Enclosure protection

5,7 A

Tel. + 49 (0) 7462/ 2005-0

CB 160 Serial No. 00-00000

Figure 2: Type plate (example of CB 160 regular unit)

Nominal temperature

Made in Germany

CE conformity marking

Electrical and electronic equipment manufactured / placed on the market in the EC after 13 August 2005 and to be disposed of in separate collection according to directive 2002/96/EC on waste electrical and electronic equipment (WEEE).

The equipment is certified in the GOST R certification system of GOSTSTANDARD Russia.

The equipment is certified by Underwriters Laboratories Inc.® according to standards CAN/CSA-C22.2 No. 61010-1, 2

Edition, 2004-07 (Electrical Equipment for Measurement,

(CB-UL only)

Control, and Laboratory Use; Part 1: General Requirements); UL 61010-1, 2 Measurement, Control, and Laboratory Use; Part 1: General Requirements); IEC 61010-1:2010, 3

Edition, 2005-07-22 (Electrical Equipment for

Edition and IEC 61010-210:2003 (Particular Requirements for Laboratory Equipment for the heating of materials).

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1.5 General safety instructions on installing and operating the CO2 incubator

With regard to operating the CO2 incubator CB and to the installation location, please observe the guideline BGI/GUV-I 850-0 on safe working in laboratories (formerly BGR/GUV-R 120 or ZH 1/119 laboratory guidelines issued by the employers’ liability insurance association) (for Germany).

BINDER GmbH is only responsible for the safety features of the unit provided skilled electricians or qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating to chamber safety are replaced in the event of failure with original spare parts.

To operate the unit, use only original BINDER accessories or accessories from third-party suppliers authorized by BINDER. The user is responsible for any risk caused by using unauthorized accessories.

CAUTION

Danger of overheating. Damage to the unit.

∅ Do NOT install the unit in unventilated recesses.

 Ensure sufficient ventilation for dispersal of the heat.

Do not operate the CO2 incubator CB in hazardous locations.

DANGER

Explosion hazard. Danger of death.

∅ Do NOT operate the unit in potentially explosive areas.

 KEEP explosive dust or air-solvent mixtures AWAY from the unit.

The CO2 incubator CB does not dispose of any measures of explosion protection.

DANGER

Explosion hazard. Danger of death.

∅ Do NOT introduce any substance into the CO2 incubator which is combustible or

explosive at working temperature.

∅ NO explosive dust or air-solvent mixture in the inner chamber.

Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature inside the chamber must lie below the flash point or below the sublimation point of the charging material. Familiarize yourself with the physical and chemical properties of the charging material, as well as the contained moisture constituent and its behavior with the addition of heat energy.

Familiarize yourself with any potential health risks caused by the charging material, the contained moisture constituent or by reaction products which may arise during the temperature process. Take adequate measures to exclude such risks prior to putting the CO

incubator into operation.

DANGER

Electrical hazard. Danger of death.

∅ The unit must NOT become wet during operation or maintenance.

The CO2 incubators were produced in accordance with VDE regulations and were routinely tested in accordance to VDE 0411-1 (IEC 61010-1).

CB (E6) 09/2013 Page 15/144

CAUTION

The glass doors and the inner chamber will become hot during operation. Danger of burning.

∅ Do NOT touch the glass doors, the inner surfaces or the charging material during

operation.

WARNING

Stability hazard. Danger of injury. Damage to the unit and the charging material. Housing cover breakaway.

∅ Do NOT climb on the lower housing cover. ∅ Do NOT load the lower housing cover with heavy objects while the unit door is open.

1.6 Precautions when working with gases

Notes on handling carbon dioxide (CO2)

Carbon dioxide (CO and therefore practically imperceptible. Vent out any CO or a suitable connection to an exhaust system. We recommend installing a CO

) in high concentrations is hazardous to health. It is colorless and almost odorless

gas that may escape via good room ventilation

WARNING

High concentration of CO2 (> 4 Vol.-%). Risk of death by suffocation. Danger of poisoning.

∅ Do NOT set up units in non-ventilated recesses.

 Ensure technical ventilation measures.  Observe the relevant regulations for handling CO

warning system

CB (E6) 09/2013 Page 16/144

t and necessary safety

ition sources in areas where oxygen enrichment is

Unit with O

Oxygen (O

control: Notes on handling oxygen (O2)

) is colorless and almost odorless and therefore practically imperceptible. It promotes burns,

which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes and hair. O

is heavier than air and may accumulate in low-lying areas.

WARNING

High concentration of O2 (> 21 % O2). Fire and explosion hazard through contact of combustible materials with O Risk of burns and other injuries.

∅ Do NOT set up units in non-ventilated recesses.

 Ensure technical ventilation measures.  Observe the relevant regulations for handling O

Take appropriate measures to prevent oxygen enrichment and fire and explosion hazards in areas where oxygen enrichment is possible.

General information for safe handling of oxygen:

• Make sure training of personnel on hazards of oxygen enrichmen measures.

• Make sure adequate labeling of all oxygen equipment and facilities.

• Make sure gas tightness of all gas connections by checking them for leaks (e.g. with leak

spray or diluted soap solution).

• Close the main valve of the source of oxygen after work when not using the chamber.

• Never lubricate O

equipment with oil or fat. Use only materials and spare parts which are

approved for use with oxygen.

• Regularly inspect fire extinguishers for proper condition.

• Set up emergency showers where oxygen enrichment is possible.

• Strictest smoking ban and no ign

possible.

• Make sure good ventilation of areas where oxygen enrichment is possible (location of the chamber and/or O

cylinders.

• Persons who may have been in a possibly oxygen-enriched atmosphere must keep away from ignition sources (flames, cigarettes, etc.) and ventilate their clothes at least 15 minutes.

• Always keep emergency routes free.

Unit with O2 control: Notes on handling nitrogen (N2)

Nitrogen (N therefore practically imperceptible. Any N

) in high concentrations is hazardous to health. It is colorless and almost odorless and

gas that may escape must be safely led out via good room

ventilation or a suitable connection to an exhaust system.

WARNING

High concentration of N2. Risk of death by suffocation.

∅ Do NOT set up units in non-ventilated recesses.

 Ensure technical ventilation measures.  Observe the relevant regulations for handling N

CB (E6) 09/2013 Page 17/144

1.7 Precautions when handling gas cylinders

General information for safe handling of gas cylinders:

• Store and use gas cylinders only in well-ventilated locations.

• Open the gas cylinder valve slowly to avoid pressure surges.

• Secure gas cylinders during storage and use against falling (chaining).

• Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them.

• Always close the valve even with apparently empty cylinders; screw on the cap when not in

use. Return gas cylinders with the valve closed.

• Do not open gas cylinders by force. Mark them when damaged.

• Protect gas cylinders against fire, e.g. do not store together with flammable liquids.

• Observe relevant regulations for dealing with gas cylinders.

Secure the gas cylinders against falling and other mechanical damage.

WARNING

Safety valve tearing off. Sudden release of the stored pressure energy. Risk of injury.

 Secure gas cylinders against falling (chaining).  Transport gas cylinders with a cylinder cart.

The valve of the gas cylinder always must be closed before screwing on or unscrewing the gas hose.

WARNING

Opening the cylinder valve when the cylinder is not connected. Sudden release of the stored pressure energy. Risk of injury.

 Close the gas cylinder valve before connecting or removing the gas hose.

After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution).

1.8 Intended use

Series CB incubators are suitable for the cultivation of mammal cells under typical conditions of approx. 37 °C / 98.6°F. The incubator permits setting defined pH conditions by common NaHCO of commercial cell media by keeping an exact CO humidity inside to avoid osmolarity increasing caused by the evaporation of the cell media.

With the unit with O cells.

Following the instructions in this operating manual and conducting regular maintenance work (chap. 19.1) are part of the intended use.

control, a variable oxygen atmosphere can additionally influence the growth of the

atmosphere inside. CB incubators guarantee high

buffer systems

Other applications are not approved.

WARNING: If customer should use a BINDER chamber running in non-supervised continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples to split such specimen or samples and store them in at least two chambers, if this is feasible.

CB (E6) 09/2013 Page 18/144

2. Unit description

The CO2 incubators CB are equipped with a multifunctional microprocessor display controller for temperature, CO degree resp. 0.1 vol.-%

The inner chamber, the pre-heating chamber and the inside of the doors are all made of stainless steel (material no. 1.4301 (V2A) in Germany). The inner surfaces are smooth and therefore easy to clean. The inner chamber is deep-drawn from one piece, polished (suitable for pharmaceutical applications) and has no welds or inaccessible corners. The hinges and the seal of the inner glass door are glued from the outside to aid cleaning of the inner chamber. When operating the chamber at high temperatures (sterilization), the impact of the oxygen in the air may cause discoloration of the metallic surfaces (yellowish-brown or blue) by natural oxidation processes. These colorations are harmless and will in no way impair the function or quality of the unit.

The perforated shelves are also made of stainless steel. You can insert a maximum of 3 (CB 60), 6 (CB

160), resp. 8 (CB 220) shelves. The housing is RAL 7035 powder-coated. All corners and edges are also completely coated.

, and O2 (unit with O2 control) levels and a digital display accurate to one-tenth of a

The heating system of the CO

incubator permits hot-air auto-sterilization at a setpoint of 187.5 °C /

369.5°F. Thus, a temperature of 180 °C / 356°F is maintained for at least 30 minutes on all internal surfaces, resulting in sterilization of the entire inner chamber. Therefore, this procedure meets all international guidelines regarding hot air sterilization, e.g. AAMI ST63, DIN 58947, European Pharmacopoeia.

Thanks to the standard safety device (class 3.1 according to DIN 12880), the set temperature is maintained in case of failure.

The gas enters the chamber via a fine filter (aseptic filter) with a high filtration efficiency that also filters the smallest particles.

A highly precise, drift-free CO

gas through a special proprietary gas mixing head developed by BINDER allows precise and

constant CO The CO

and O2 sensors

concentrations for long periods. This creates optimum growth conditions for cultures.

incubator is also available with O2 control in addition to CO2 control.

Fast reaction times, maximum accuracy and selectivity characterize the CO the CB incubator series. The accuracy of the CO

infrared measuring system in combination with the permanent mixture of

measuring procedure of

measuring system is based on a double-beam

infrared measuring cell with NDIR (non-dispersive infrared) sensor, which continuously regulates to a reference value. Therefore, disturbance variables and aging phenomena in the measuring system are almost completely eliminated, so that this measuring system, in contrast to other measuring procedures, remains practically drift-free between calibrations and is entirely selective for CO

and

humidity. The sensor is built into the incubator and can be sterilized. The O The accuracy of the indicated values of CO

pressure (approx. 0.08 vol.-% per 10 mbar / 0.15 psi). To compensate for this effect in the CO

sensor is a semiconductor gas sensor with ZrO2 ceramic.

and O2 (unit with O2 control) depends on the ambient air

measurement, the controller measures the ambient air pressure and automatically includes it in the calculation.

The CO

incubators CB are equipped with an Ethernet interface for computer communication, e.g. with

the communication software APT-COM™ 3 DataControlSystem (option, chap. 15.1). For further options, see chap. 22.5.

Temperature range: 7 °C / 12.6 °F above ambient temperature up to +60 °C / 140 °F

range: 0 vol.-% up to 20 vol.-%)

range (unit with O2 control): 0.2 vol.-% up to 95 vol.-%

CB (E6) 09/2013 Page 19/144

2.1 Unit overview

Instrument panel with

microprocessor controller

T4.12 and USB interface

Door handle

Unit door

Main power switch

Figure 3: CO2 incubator CB (example: model CB 160)

2.2 Instrument panel

5,7" controller display

Context-sensitive buttons

USB interface

Operating button

Pilot lamp: ready for operation

Figure 4: Instrument panel with microprocessor controller T4.12 and USB interface

CB (E6) 09/2013 Page 20/144

FRO NT

(A)

(I) ( ( (

(B) (C) (D) (E)

(G)

(H)

2.3 Inner chamber

J) K) L)

Figure 5: CB 160 with O

control and options

CB (E6) 09/2013 Page 21/144

(B)

(C)

(D)

(D2) (F)

(G)

(E)

Figure 6: CB 160 / CB 220 with O

control and options

(A) Instrument panel with microprocessor controller T4.12, indicating temperature and CO

(unit with O2 control)”

(B) Connection socket for extra-low voltage supply (option, chap. 15.6) (C) CO (D) Gas mixing head CO (D2) Additional gas mixing head O

and humidity sensor

/ N2 (unit with O2 control)

(E) Pt 100 temperature probe (F) O

sensor (unit with O2 control)

(G) Internal socket 230V (max. 3 A) (option, chap. 15.4) (H) Perforated shelves, made of stainless steel (I) Measuring access port (J) Inner glass doors (K) Permadry™ water pans (L) Lower housing cover

as well as

CB (E6) 09/2013 Page 22/144

2.4 Control panel on the rear of the unit

(1) (2) (3) (6) (4) (5)

(7) (8) (9) (10)

Figure 7: Rear control panel CB with O

control and options

(1) Miniature fuse (2) External socket for extra-low voltage supply (option for CB 160 / CB 220, chap. 15.6) (3) DIN socket for analog outputs 4-20 mA (option, chap. 15.5) (4) Quick acting closure socket for CO (5) Quick acting closure socket for O (6) Quick acting closure socket for N

(unit with O2 control)

(7) Socket for IEC connector plug for power cable (8) Ethernet interface for computer communication (9) DIN-socket for zero-voltage relay alarm outputs (10) RS 422 interface for computer communication (option)

CB (E6) 09/2013 Page 23/144

3. Completeness of delivery, transportation, storage, and installation

3.1 Unpacking, and checking equipment and completeness of delivery

After unpacking, please check the unit and its optional accessories, if any, based on the delivery receipt for completeness and for transportation damage. Inform the carrier immediately if transportation damage has occurred.

The final tests of the manufacturer may have caused traces of the shelves on the inner surfaces. This has no impact on the function and performance of the unit.

Please remove any transportation protection devices and adhesives in/on the unit and on the doors and remove the operating manuals and accessory equipment.

Remove any protective lamination sheet on the inner metal surfaces prior to commissioning.

CAUTION

Sliding or tilting of the unit. Damage to the unit. Risk of injury by lifting heavy loads.

 Do NOT lift or transport the unit using the door handle, the door or the lower housing.  Lift the unit from the pallet at the four lower corners with the aid of four people.

If you need to return the unit, please use the original packing and observe the guidelines for safe lifting and transportation (chap. 3.2).

For disposal of the transport packing, see chap. 20.1.

Note on second-hand units (Ex-Demo-Units):

Second-hand units are units that were used for a short time for tests or exhibitions. They are thoroughly tested before resale. BINDER ensures that the chamber is technically sound and will work flawlessly.

Second-hand units are marked with a sticker on the unit door. Please remove the sticker before commissioning the unit.

3.2 Guidelines for safe lifting and transportation

After operation, please observe the guidelines for temporary decommissioning (chap. 20.2). Empty the Permadry™ water pan before moving the incubator. In case of any spilling of the contents, shut down the incubator and dry it out carefully and completely.

CB (E6) 09/2013 Page 24/144

CAUTION

Sliding or tilting of the unit. Damage to the unit. Risk of injury by lifting heavy loads.

 Transport the unit in its original packaging only.  For moving or shipping, secure the unit with transport straps.

∅ Do NOT lift or transport the unit using the door handle, the door or the lower housing.

 Lift the unit at the four lower corners with the aid of 4 people and place it on a rolling

pallet.

 Move the unit to the desired location and lift it from the rolling pallet with the aid of four

people.

• Permissible ambient temperature range during transport: 10 °C / 14 °F to +60 °C / 140 °F.

You can order transport packing for moving or shipping purposes from BINDER service.

3.3 Storage

Intermediate storage of the unit is possible in a closed and dry room. Observe the guidelines for temporary decommissioning (chap. 20.2).

• Permissible ambient temperature range during storage: -10 °C / 14 °F to +60 °C / 140 °F.

• Permissible ambient humidity: max. 70 % r.H., non-condensing

When after storage in a cold location you transfer the unit to its warmer installation site, condensation may form. Before start-up, wait at least one hour until the chamber has attained ambient temperature and is completely dry.

3.4 Location of installation and ambient conditions

Notes on the location of installation

Set up the CO The chambers are designed for setting up inside a building (indoor use).

Freestanding CB incubators are suitable for installation on tables or on the optionally available stand (height 200 mm / 0.5 ft). Note: The site of installation must be capable of supporting the unit’s weight (see technical data, chap.22.4).

Align the unit using a spirit level to ensure even covering of the cell-cultures with the medium. For this purpose, manually adjust the four incubator feet.

incubators can be stacked on top of each other (two units maximum). For safe stacking that is easy

to maintain, use the original BINDER stacking stand (chap. 15.8.1) or the stacking adapter (chap. 15.8.2).

incubator on a flat, even surface, free from vibration and in a well-ventilated, dry location.

CAUTION

Sliding of stacked unit. Damage to the units.

 When stacking, use rubber pads (Art. No. 8012-0376) for the feet of the upper

incubator.

To completely separate the unit from the power supply, you must disconnect the power plug. Install the unit in a way that the power plug is easily accessible and can be easily pulled in case of danger.

In order to avoid contamination, never place the unit directly on the floor.

CB (E6) 09/2013 Page 25/144

CAUTION

Danger of overheating. Damage to the unit.

∅ Do NOT set up units in non-ventilated recesses.

 Ensure sufficient ventilation for dispersal of the heat.

Do not install or operate the CO2 incubator CB in potentially explosive areas.

DANGER

Explosion hazard. Danger of death.

∅ Do NOT operate the unit in potentially explosive areas.

 KEEP explosive dust or air-solvent mixtures AWAY from the vicinity of the unit.

Ambient conditions

• Permissible ambient temperature range for operation: +18 °C / 64.4 °F to +30 °C / 86 °F

At elevated ambient temperature values, fluctuations in temperature can occur.

• Ideal ambient temperature: at least 7 °C / 12.6 °F below the intended working temperature. E.g.,

working temperature 37 °C / 98.6 °F = ambient temperature 30 °C / 86 °F and lower. In the event of working temperatures of less than 7 °C / 12.6 °F above the ambient temperature, the setpoint can be exceeded.

The ambient temperature should not be substantially higher than the indicated ambient temperature of 22 ±3 °C / 71.6 ±5.4 °F to which the specified technical data relates. For other ambient conditions, deviations from the indicated data are possible.

Avoid direct solar radiation on the unit.

• Permissible ambient humidity: 70 % r.H. max., non-condensing.

• Installation height: max. 2000 m / 6561.7 ft above sea level.

• Wall distances: rear 100 mm / 3.94 in, sides 50 mm / 1.97 in.

CB (E6) 09/2013 Page 26/144

Notes on handling carbon dioxide (CO

Carbon dioxide (CO

) in high concentrations is hazardous to health. It is colorless and almost odorless

and therefore practically imperceptible. Vent out any CO or a suitable connection to an exhaust system. We recommend installing a CO

)

gas that may escape via good room ventilation

warning system.

WARNING

High concentration of CO2 (> 4 Vol.-%). Danger of death by suffocation. Danger of poisoning.

∅ Do NOT set up units in non-ventilated recesses.

 Ensure technical ventilation measures.  Observe the relevant regulations for handling CO

Observe the occupational exposure limit OEL for CO2 set by the national authorities (formerly maximum permitted workplace concentration). Check compliance when operating all units located in the room.

• OEL for Germany: 5000 ml/m

• CO

lost with each opening the door: about 16.4 g, i.e. 0.0084 cubic meters / 0.296 cubic feet (under

(ppm) = 0,5 Vol.-%

normal pressure)

• CO

lost during 12h at 5 vol.-% without opening the door: approx. < 2 g, i.e. 0.001 cubic meter / 0.035

cubic feet (under normal pressure 1013 mbar / 14.7 psi)

An example of how to evaluate laboratory volume and air change rate:

Question: Is an air change rate of 1/h sufficient for a lab with a volume of 100 cubic meters /

3,531.5 cubic feet with 10 incubators CB, opened 4 times per hour?

Calculation: CO

concentration = CO2 lost by opening the door, multiplied by 10 units, multiplied

by opening the door 4 times per hour, divided by lab volume

0.0084 cubic meters x 10 x 4 div. 100 cubic meters = 0.00336, i.e. 0.336 % or 3360 ppm.

0.296 cubic feet x 10 x 4 div. 3,531.5 cubic feet = 0.00336, i.e. 0.336 % or 3360 ppm. Result: The maximum permissible value of 5000 ppm is not exceeded under these operation

conditions.

Unit with O

Oxygen (O

control: Notes on handling oxygen (O2)

) is colorless and almost odorless and therefore practically imperceptible. It promotes burns,

which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes and hair. O

is heavier than air and may accumulate in low-lying areas.

WARNING

High concentration of O2 (> 21 % O2). Fire and explosion hazard through contact of combustible materials with O Risk of burns and other injuries.

∅ Do NOT set up units in non-ventilated recesses.

 Ensure technical ventilation measures.  Observe the relevant regulations for handling O

Take appropriate measures to prevent oxygen enrichment and fire and explosion hazards in areas where oxygen enrichment is possible.

CB (E6) 09/2013 Page 27/144

t and necessary safety

Strictest smoking ban and no ignition sources in areas where oxygen enrichment is

from ignition sources (flames, cigarettes, etc.) and ventilate their clothes at least 15

General information for safe handling of oxygen:

• Make sure training of personnel on hazards of oxygen enrichmen measures.

• Make sure adequate labeling of all oxygen equipment and facilities.

• Make sure gas tightness of all gas connections by checking them for leaks (e.g. with leak

spray or diluted soap solution).

• Close the main valve of the source of oxygen after work when not using the chamber.

• Never lubricate O

equipment with oil or fat. Use only materials and spare parts which are

approved for use with oxygen.

• Regularly inspect fire extinguishers for proper condition.

• Set up emergency showers where oxygen enrichment is possible.

•

possible.

• Make sure good ventilation of areas where oxygen enrichment is possible (location of the chamber and/or O

cylinders.

• Persons who may have been in a possibly oxygen-enriched atmosphere must keep away

minutes.

• Always keep emergency routes free.

Unit with O

Nitrogen (N therefore practically imperceptible. Any N

control: Notes on handling nitrogen (N2)

) in high concentrations is hazardous to health. It is colorless and almost odorless and

gas that may escape must be safely led out via good room

ventilation or a suitable connection to an exhaust system.

WARNING

High concentration of N2. Risk of death by suffocation.

∅ Do NOT set up units in non-ventilated recesses.

 Ensure technical ventilation measures.  Observe the relevant regulations for handling N

CB (E6) 09/2013 Page 28/144

Figure 8: Permadry™ water pan CB 160 / CB 220

Figure 9: Permadry™ water pan CB 60

4. Installation and connections

4.1 Shelves

You can put the shelves in different positions at the line of channel beads in the inner chamber. Hold the shelf straight and then insert it so it will go smoothly inside the unit.

Permitted shelf loads:

Maximum load of one single shelf: 10 kg / 22 lb Maximum total load of all shelves: 30 kg / 66 lb

4.2 Permadry™ water pan

The Permadry™ system developed by BINDER is an effective and easy to handle system that ensures high humidity inside the incubator without any condensation forming on the inner surfaces. The Permadry™ water pan consists of two pans in which the outer one is heated and the inner one cooled. With the slight difference of temperature caused by that cooling, the central pan is the specific point for condensation of the surplus humidity. Therefore, all other inner surfaces remain dry.

• Put the Permadry™ water pan on the bottom of the inner chamber in a way that both notches lock into

place.

• The front side of the Permadry™ water pan is marked “FRONT”.

Figure 10: Letters “FRONT” indicating the front of the Permadry™ water pan

• Please make sure that the Permadry™ water pan has firm contact to the inner chamber bottom and

rests tightly on it.

• Fill only the outer pan with distilled, sterilized water up to the filling level marking on the edge of the

inner pan. Maximum filling quantity of the outer pan: CB 60: approx. 0.7 liters, CB 160 and CB220): approx.

2 liters.

Figure 11: Filling height line of the outer basin CB 160 / CB 220

CB (E6) 09/2013 Page 29/144

• We recommend cleaning and refilling the pans 2 to 3 times a week. For evacuation, remove the

Permadry™ water pan.

• We recommend using distilled, sterile water to achieve optimum growth results. Any corrosive damage

that may arise following the use of water of different quality or by additives is excluded from the liability agreement.

• If required, you can add microbiologically inhibiting substances such as copper chips, copper sulfate

or ethylene diamine tetra-vinegar acid (EDTA) in a concentration of 1 to 5 mmol/l.

Empty the Permadry™ water pan before moving the incubator. In case of the contents spilling, immediately shut down the incubator and dry it carefully and completely.

4.3 Gas connections

General information for safe handling of gas cylinders:

• Store and use gas cylinders only in well ventilated areas.

• Open the gas cylinder valve slowly to avoid pressure surges.

• Secure gas cylinders during storage and use against falling (chaining).

• Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them.

• Always close the valve even with apparently empty cylinders; screw on the cap when not in

use. Return gas cylinders with the valve closed.

• Do not open gas cylinders by force. Mark them when damaged.

• Protect gas cylinders against fire, e.g. do not store together with flammable liquids.

• Observe relevant regulations for dealing with gas cylinders.

Secure the gas cylinder against falling and other mechanical damage.

WARNING

Safety valve tearing off. Sudden release of the stored pressure energy. Risk of injury.

 Secure gas cylinders against falling (chaining).  Transport gas cylinders with a cylinder cart.

The valve of the gas cylinder always must be closed before screwing on or unscrewing the gas hose.

WARNING

Opening the cylinder valve when the cylinder is not connected. Sudden release of the stored pressure energy. Risk of injury.

 Close the gas cylinder valve before connecting or removing the gas hose.

After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution).

CB (E6) 09/2013 Page 30/144

4.3.1 Connection of the CO2 gas cylinder

Carbon dioxide (CO2) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Vent out any CO or a suitable connection to an exhaust system. We recommend installing a CO

High concentration of CO2 (> 4 Vol.-%). Danger of death by suffocation.

∅ Do NOT set up units in non-ventilated recesses.

 Ensure technical ventilation measures.  Observe the relevant regulations for handling CO

The CO2 gas necessary for operation must have a technical grade of 99.5 %.

The gas connections must be established by qualified personnel who are trained in handling the respective gases and familiar with the required safety measures.

gas that may escape via good room ventilation

WARNING

warning system.

The following steps are required:

Ensuring the correct CO

output pressure

A gas supply pressure above 2.5 bar / 36 psi will result in unit damage.

Use a pressure reducer and make sure to avoid any excessive outlet pressure when connecting the gas hose to the incubator.

The real outlet pressure of gas cylinders, sets of gas cylinders or central gas supplies am on the second manometer must not exceed 2.5 bar / 36 psi.

CAUTION

Excessive outlet pressure > 2.5 bar / 36 psi. Damage to the unit.

∅ The outlet pressure must NOT exceed the indicated value of 2.5 bar / 36 psi.

 Before connecting, check the outlet pressure on the pressure reducer of the

cylinder.

 Adjust the outlet pressure to 2.0 bar / 29 psi above the ambient pressure.

Observe the correct outlet pressure also when replacing the gas cylinders.

Establishing the connection to the incubator

Connect the supplied gas hose (internal diameter 6 mm / 0.24 inches) to the pressure reducer of the gas cylinders or central gas supply and secure the connection with the supplied hose clamp.

Connect the pre-assembled hose nozzle of the gas hose to the quick acting closure socket (4) DN 6 on the unit rear, as described in chap. 4.3.4.

Leak test

After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution).

CB (E6) 09/2013 Page 31/144

The recovery times of the gas concentrations inside the chamber after opening the door are indicated in the technical data (chap. 22.4) and refer to a connection pressure of 2.0 bar / 29 psi. Decreasing supply pressure will result in longer recovery times.

Conversion table for gas inlet pressures, bar – psi, see chap. 22.8.

4.3.2 Connection of the O2 gas cylinder (unit with O2 control)

Note: Do not connect the O2 cylinder or disconnect the gas supply (by pulling off the gas hose) when operating at setpoints below 19 vol.-% O

Oxygen (O2) is colorless and almost odorless and therefore practically imperceptible. It promotes burns, which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes and hair. O

is heavier than air and may accumulate in low-lying areas.

WARNUNG

High concentration of O2 (> 21 % O2). Fire and explosion hazard through contact of combustible materials with O Risk of burns and other injuries.

∅ Do NOT set up units in non-ventilated recesses.

 Ensure technical ventilation measures.  Observe the relevant regulations for handling O

The O2 gas necessary for operation must have a technical grade of 99.5 %.

The gas connections must be established by qualified personnel who are trained in handling the respective gases and familiar with the required safety measures.

The following steps are required:

Ensuring the correct O

output pressure

A gas supply pressure above 2.5 bar / 36 psi will result in unit damage.

Use a pressure reducer and make sure to avoid any excessive outlet pressure when connecting the gas hose to the incubator.

The real outlet pressure of gas cylinders, sets of gas cylinders or central gas supplies am on the second manometer must not exceed 2.5 bar / 36 psi.

CAUTION

Excessive outlet pressure > 2.5 bar / 36 psi. Damage to the unit.

∅ The outlet pressure must NOT exceed the indicated value of 2.5 bar / 36 psi.

 Before connecting, check the outlet pressure on the pressure reducer of the

cylinder.

 Adjust the outlet pressure to 2.0 bar / 29 psi above the ambient pressure.

Observe the correct outlet pressure also when replacing the gas cylinders.

CB (E6) 09/2013 Page 32/144

Establishing the connection to the incubator

Connect the supplied gas hose (internal diameter 6 mm / 0.24 inches) to the pressure reducer of the gas cylinders or central gas supply and secure the connection with the supplied hose clamp.

Connect the pre-assembled hose nozzle of the gas hose to the quick acting closure socket (5) DN 6 on the unit rear, as described in chap. 4.3.4.

Leak test

After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution).

Conversion table for gas inlet pressures, bar – psi, see chap. 22.8.

4.3.3 Connection of the N2 gas cylinder (unit with O2 control)

Nitrogen (N2) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Any N ventilation or a suitable connection to an exhaust system.

gas that may escape must be safely led out via good room

WARNING

High concentration of N2. Risk of death by suffocation.

∅ Do NOT set up units in non-ventilated recesses.

 Ensure technical ventilation measures.  Observe the relevant regulations for handling N

The N2 gas necessary for operation must have a technical grade of 99.5 %.

The gas connections must be established by qualified personnel who are trained in handling the respective gases and familiar with the required safety measures.

The following steps are required:

Ensuring the correct N

output pressure

A gas supply pressure above 2.5 bar / 36 psi will result in unit damage.

Use a pressure reducer and make sure to avoid any excessive outlet pressure when connecting the gas hose to the incubator.

The real outlet pressure of gas cylinders, sets of gas cylinders or central gas supplies am on the second manometer must not exceed 2.5 bar / 36 psi.

CB (E6) 09/2013 Page 33/144

CAUTION

Excessive outlet pressure > 2.5 bar / 36 psi. Damage to the unit.

∅ The outlet pressure must NOT exceed the indicated value of 2.5 bar / 36 psi.

 Before connecting, check the outlet pressure on the pressure reducer of the

cylinder.

 Adjust the outlet pressure to 2.0 bar / 29 psi above the ambient pressure.

Observe the correct outlet pressure also when replacing the gas cylinders.

Establishing the connection to the incubator

Connect the supplied gas hose (internal diameter 6 mm / 0.24 inches) to the pressure reducer of the gas cylinders or central gas supply and secure the connection with the supplied hose clamp.

Connect the pre-assembled hose nozzle of the gas hose to the quick acting closure socket (6) DN 6 on the unit rear, as described in chap. 4.3.4.

Leak test

After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution).

Conversion table for gas inlet pressures, bar – psi, see chap. 22.8.

4.3.4 Connecting the gas hose to the unit rear (for CO2, O2, N2)

The procedure of connecting the gas hose to the unit rear is the same for any gas connection. All quick acting closure sockets (CO

, and N2 and O2 on unit with O2 control) are degreased and supplied with a

FKM gasket.

Note for units with O

connection (unit with O

control: The quick acting closure socket and the hose nozzle for the O2

control) must be degreased.

WARNING

Fire and explosion hazard through contact of fat with O2. Risk of burns and other injuries.

∅ All connection parts for the O

The gas hose, which will be used to establish the connection to a gas cylinder, is already attached to the hose nozzle and secured by a hose clamp. Plug the hose nozzle into the corresponding quick acting closure socket (a) located at the rear of the unit. This quick acting closure socket is closed by a rubber cover (b).

connection must be degreased.

Only use the supplied hose nozzle to connect to the quick acting closure socket. Otherwise, the quick acting closure socket may leak, and/or it may become impossible to

connect the original hose nozzle. In this case, please contact BINDER Service.

CB (E6) 09/2013 Page 34/144

Gas cylinder connection kits are available for CO2 (Art. No. 8012-0014), O2

(a)

(c)

(b)

Remove the rubber cover (b) by pulling it off.

Unit rear

Figure 12: Connecting the hose lead to the gas cylinder

Now fit the hose nozzle (c) in the quick acting closure socket. To remove the connection, pull the hose nozzle off the quick acting closure socket.

4.3.5 Gas cylinder connection kits (option)

(Art. No. 8012-0015) and N2 (Art. No. 8012-0016). The connection kit includes the following parts for connecting a gas cylinder

to the CO

• Pressure reducer with manometers for cylinder pressure (high pressure gauge) and outlet pressure (low pressure gauge)

• 5 m pressure hose with pre-assembled hose nozzle for quick acting closure socket

• 1 hose clamp to connect the gas hose to the pressure reducer

Figure 13: Gas cylinder connection kit

Instructions 7001-0249 included with the connection kit describe connecting and setting the gas cylinder connection kit.

incubator:

Excessive outlet pressure > 2.5 bar / 36 psi. Damage to the unit.

∅ The outlet pressure must NOT exceed the indicated value of 2.5 bar / 36 psi.

 Before connecting, check the outlet pressure on the pressure reducer of the cylinder.  Adjust the outlet pressure to 2.0 bar / 29 psi above the ambient pressure.

The gas connections must be established by qualified personnel who are trained in handling the respective gases and familiar with the required safety measures.

CB (E6) 09/2013 Page 35/144

CAUTION

4.4 Electrical connection

The CO2 incubator comes with an IEC connector plug.

Model

Art. No. (x = 0 or 1)

Power plug

Voltage +/-10 %

Power frequency

Unit fuse

CB 60 9x40-0088 Shock-proof plug 230 V (1N~) 50/60 Hz 16 A

CB 60-UL 9x40-0089 NEMA 5-15P 100-120 V (1N~) 60 Hz 16 A

CB 60 with O

control

CB 60-UL with O

control

9x40-0090 Shock-proof plug 230 V (1N~) 50/60 Hz 16 A

9x40-0091 NEMA 5-15P 100-120 V (1N~) 60 Hz 16 A

CB 160 9x40-0092 Shock-proof plug 230 V (1N~) 50/60 Hz 16 A

CB 160-UL 9x40-0093 NEMA 5-20P 100-120 V (1N~) 60 Hz 16 A

CB 160 with O

control

CB 160-UL with O

control

9x40-0094 Shock-proof plug 230 V (1N~) 50/60 Hz 16 A

9x40-0095 NEMA 5-20P 100-120 V (1N~) 60 Hz 16 A

CB 220 9x40-0096 Shock-proof plug 230 V (1N~) 50/60 Hz 16 A

CB 220-UL 9x40-0097 NEMA 5-20P 100-120 V (1N~) 60 Hz 16 A

CB 220 with O

control

CB 220-UL with O

control

9x40-0098 Shock-proof plug 230 V (1N~) 50/60 Hz 16 A

9x40-0099 NEMA 5-20P 100-120 V (1N~) 60 Hz 16 A

• Prior to connection and start-up, check the power supply voltage. Compare the values to the specified data located on the unit’s type plate (left unit side, bottom right-hand, see chap. 1.4).

• When connecting, please observe the regulations specified by the local electricity supply company as well as the VDE directives (for Germany).

• Pollution degree (acc. to IEC 61010-1): 2

• Over-voltage category (acc. to IEC 61010-1): II

CAUTION

Danger of incorrect power supply voltage. Damage to the equipment.

 Check the power supply voltage before connection and start-up.  Compare the power supply voltage with the data indicated on the type plate.

5. Start up

After connecting the supply lines (chap. 4.4) turn on the chamber by the main power switch. The pilot lamp shows the unit is ready for operation.

Observe a delay time of about 30s between turning Off and On again. Otherwise an initialization problem may occur.

Note that the chamber is in stand-by mode when the main power switch has been turned on and yet the controller display is dark. Turn on the unit by pressing any controller button.

Warming chambers may release odors in the first few days after commissioning. This is not a quality defect. To reduce odors quickly we recommend heating up the chamber to its nominal temperature for one day and in a well-ventilated location.

5.1 Equilibration time

Temperature: Equilibration time is approx. 1 hour.

: After approx. 5 minutes, the CO2 concentration equilibrates automatically to the pre-set value of 5

vol.-% CO

(unit with O2 control): After a delay of 10 minutes, the ambient oxygen concentration of approx. 20.7

vol.-% is displayed. During the first 10 minutes, O

control is not defined. Then O2 is regulated to the

displayed setpoint value of 20.7 vol.-%.

5.2 Factory settings

The unit is supplied with the following basic preset parameters:

• Temperature setpoint 37 °C / 98.6°F

• CO

• O

• Safety controller class 3.1 38.5 °C / 101.3°F

• Sterilization temperature 187.5 °C / 369.5°F

• Audible alarm signal (buzzer) turned on

concentration 5 vol.-%

concentration (unit with O2 control) 20.7 vol.-%

The set temperature determines the target working temperature in the inner chamber, i.e. set value 37 °C / 98.6°F = target working temperature 37 °C / 98.6°F. The same is valid for the CO (unit with O

control). For the hot-air sterilization, the set value is 187.5 °C / 369.5°F and cannot be

and O2 concentration

changed.

As long as there is a difference between the actual and set value shown in the display, adequate operation of the unit is not guaranteed.

CB (E6) 09/2013 Page 38/144

6. Functional overview of the T4.12 chamber controller

The T4.12 chamber controller controls the following values inside the CO2 incubator:

• Temperature in °C (range by 7 °C / 12.6 °F above ambient temperature up to +60 °C / 140 °F)

• Carbon dioxide concentration in vol.-% (range 0 vol.-% up to 20 vol.-%)

Unit with O

• Oxygen concentration in vol.-% (range 0.2 vol.-% up to 95 vol.-%)

control in addition:

You can enter the desired setpoint values in fixed value operating mode in the display controller. The controller also offers various notifications and alarm messages with visual and audible indication, a trace file and remote alarms via e-mail. Programming can be done directly through the keypad of the controller or graphically through the software APT-COM™ 3 DataControlSystem (option, chap. 15.1) specially developed by BINDER.

Temperature values

values

Humidity value

values

Actual values Setpoint values

Icons: Controller operating in “fixed value” operating mode

Figure 15: T4.12 microprocessor controller, initial view in “Fixed value” controller mode

(sample values, unit with O

control)

CB (E6) 09/2013 Page 39/144

Fixed value

Setpoint

Actual value

37.0

37.2

5.0

85.5

20.7

Fixed value

..\ Menu

Controller mode

Event list

Alarms

Setpoints

Safety controller

Import/Export

Hot-air sterilization

Humidity control

6.1 Menu structure

08.03.2013 05:05:06

Temperature [°C]

CO2 [Vol.-%]

Humidity [%rH]

O2 [Vol.-%]

User Quick menu

From the Initial view you have access to different menus using the menu buttons “User”, “Quick menu”, or “Menu”. From there you can access the desired control functions. To do this, select the function by turning the operating button and press the operating button to confirm the selection.

In any menu, you can return to the previous display pressing the "Close" button or to the initial view with the "Home" button.

Depending on the logged-in user or administrator, the available menu functions may vary. These instructions present the functions which are available to the logged-in administrator.

Initial view (sample values, unit with O2 control). Press the desired menu button.

6.1.1 General menu

The general menu provides access to all setting functions of the controller, a graphical display of the measured values, and the possibility to read and give out data via the USB interface. In addition, supporting functions like a settings wizard or a contact page are available.

08.03.2013 05:05:06

General menu

Close Home

Turn the operating button to see additional menu items.

CB (E6) 09/2013 Page 40/144

Fixed value

..\ Menu

Settings

Measurement chart

Interior socket (optional)

Sensor adjustment

Service contact

System information

Fixed value

..\ Quick menu

Measurement chart

Active alarms

Temperature setpoint

CO2 setpoint

O2 setpoint

Safety controller setpoint

Hot-air sterilization

08.03.2013 05:05:06

General menu (next page)

Close Home

Controller mode

Event list

Alarms

Setpoints

Safety controller

Import/Export

Hot-air sterilization

Humidity control

Settings

Measurement chart

Interior socket (optional)

Sensor adjustment

Service contact

System information

Switching between the operating modes “control off” or “fixed value”, chap.

6.2.1

Display of status information and errors, chap. 12

Alarm settings, chap. 11.5

Setpoint entry, chap. 7.4

Setting the safety controller, chap. 14.2

Data transfer via USB interface, chap. 10

Performing a hot-air sterilization at 180 °C / 356 °F, chap. 18.3

Setting the humidity control of the Permadry™ system, chap.6.4

General controller settings, chap. 9

Graphical display of the measured values, chap. 13

Turning on / off the interior socket voltage (option), chap. 6.5

Adjustment menu for single-point and two-point adjustments (for Service)

Service information

Chamber information (model, name, serial no., firmware etc.)

6.1.2 Quick menu

The Quick menu provides fast access to frequently used functions.

Close Home

CB (E6) 09/2013 Page 41/144

08.03.2013 05:05:06

“Quick menu”. (item “O

unit with O

setpoint” only coming with

control)

Fixed value

..\ User

Key lock

Show event list

Measurement chart Graphical display of the measured values, chap. 13

Active alarms Alarm settings, chap. 11.5

Temperature setpoint Temperature setpoint entry, chap. 7.3

CO2 setpoint CO2 setpoint entry, chap. 7.3

O2 setpoint O2 setpoint, chap. 7.3

Safety controller setpoint Setting the safety controller setpoint, chap. 14.2.2

Hot-air sterilization

Performing a hot-air sterilization at 180 °C / 356 °F, chap. 18.3

6.1.3 User menu

The user menu includes the key lock function and provides quick access to the event list. The key lock function serves to block the access to the controller. An overview of logon, logoff, and other

events is given in the event list.

08.03.2013 05:05:06

“User” menu

Close Home

Key lock Configuring the key lock function, chap. 8

Show event list Displaying the event list, chap. 12

CB (E6) 09/2013 Page 42/144

Fixed value

..\ Menu

Controller mode

Event list

Alarms

Setpoints

Safety controller

Import/Export

Hot-air sterilization

Humidity control

Fixed value

..\ Controller mode

Control off

Fixed value

O2 control

6.2 Operating modes

In the “control off” mode (chap. 6.2.1), the controller is non-functional and displays only the actual values. There is no heating or refrigeration and no inlet of CO temperature approximates the ambient value.

You can enter the desired setpoint values in “fixed value” mode (chap. 7). The controller then operates as a fixed-point control, i.e., it reaches and maintains the defined setpoints until the next manual change.

6.2.1 Activating the “control off” mode or change to “fixed value” operating mode

To select the “control off” or “fixed value” operating mode, go to Menu > Controller mode

08.03.2013 05:05:06

General menu. Select “Controller Mode”

and press the operating button.

or O2 (unit with O2 control). The

Close Home

08.03.2013 05:05:06

Close Home

Submenu “Controller Mode”. Select the desired controller mode

“Control off” or “Fixed value” and press the operating button.

CB (E6) 09/2013 Page 43/144

Control off

..\ Menu

Controller mode

Event list

Alarms

Setpoints

Safety controller

Import/Export

Hot-air sterilization

Humidity control

Control off

Setpoint

Actual value

22.2

0.3

85.5

08.03.2013 05:05:06

General menu. The controller mode “Fixed value” or “Control off” is

indicated in the display headline.

Close Home

Go back to the initial view with “Home”.

08.03.2013 05:05:06

Temperature [°C]

CO2 [Vol.-%]

Humidity [%rH]

User Quick menu

The controller is non-functional, i.e., there is no heating or CO2 entry or O2 entry (unit with O2 control).

Initial view in “Control off” mode (sample values)

CB (E6) 09/2013 Page 44/144

Hyperoxic range

(>22 vol.-% O2)

Hypoxic range

(<20 vol.-% O2)

Hyperoxic and

hypoxic ranges

6.3 Deactivating the O2 control and O2/N2 pressure alarms (unit with O2 control)

6.3.1 Required gas supply of the unit with O2 control

In the unit with O2 control, cell growth can be additionally influenced by a variable oxygen content. Depending on the required range of O2 concentration, the type of gas which is required may vary:

• Hyperoxic range (> 22 vol.-% O

As compared to the ambient air (O increased, no nitrogen is required for the O cylinder. You can deactivate the N

)

concentration of 20.9 vol.-%) only the O2 concentration needs to be

pressure alarm in the menu “Menu > Controller mode > O2 control”

control. Therefore, it is not necessary to connect a N2

(chap. 6.3.2).

• Hypoxic range (< 20 Vol.-% O As compared to the ambient air (O

be increased, no oxygen is required for the O cylinder. You can deactivate the O

)

concentration of 20.9 vol.-%) only the N2 concentration needs to

pressure alarm in the menu “Menu > Controller mode > O2

control. Therefore, it is not necessary to connect an O2

control” (chap. 6.3.2).

• Hyperoxic and hypoxic range or range from 20 vol.-% O N

and O2 are required for O2 control. It is necessary to connect all gas supplies.

• Operation without O You can deactivate the O

With this setting, no alarm messages for O

control

control in the menu “Menu > Controller mode > O2 control” (chap. 6.3.2).

and N2 (pressure or concentration) will be issued.

up to 22 vol.-% O2

Since neither nitrogen nor oxygen are required, it is not necessary to connect an O

and N2 pressure alarms will be issued with deactivated O2 control.

The zero-voltage relay alarm contact (chap. 11.6) for messages of deviation of the O will also not be triggered.

or N2 cylinder. No

concentration

Overview

No O2 control

off O2 control (with O2 concentration alarm)

active active active

Connection of O2 cylinder connected not connected connected not connected

off

O2 pressure alarm active

active off

Connection of N2 cylinder not connected connected connected not connected

off

N2 pressure alarm

active active off

CB (E6) 09/2013 Page 45/144

Fixed value

..\ O2 control

O2 control: On

O2 pressure alarm: On

N2 pressure alarm: On

Fixed value

Setpoint

Actual value

37.0

37.2

5.0

85.5

20.7

Fixed value

Setpoint

Actual value

37.0

37.2

5.0

85.5

25.0

6.3.2 Activating / deactivating the O2 control and O2 / N2 pressure alarms

To activate or deactivate the O2 control and O2 / N2 pressure alarms, go to

Menu > Controller mode > O2 control

08.03.2013 05:05:06

Close Home

Go back to the initial view with “Home”.

08.03.2013 05:05:06

Temperature [°C]

CO2 [Vol.-%]

Humidity [%rH]

O2 [Vol.-%]

Submenu “O2 control”. The current settings are displayed. To change the settings, press the operating button. The modified settings are displayed

“O2 control: On” = O “O2 control: Off” = O “O2 pressure alarm: On” or “N2 pressure alarm: On”

= the corresponding pressure alarm is activated. “O2 pressure alarm: Off” or “N2 pressure alarm: Off”

= the corresponding pressure alarm is deactivated.

Initial view with deactivated O2 control (sample values).

Also the pressure alarms for O deactivated.

control is active.

control is deactivated.

and N2 are

User Quick menu

To enter the O2 setpoint, first activate the O2 control again.

With active O

Temperature [°C]

CO2 [Vol.-%]

Humidity [%rH]

O2 [Vol.-%]

User Quick menu

control, the pressure alarms for O2 and N2 can be deactivated individually.

08.03.2013 05:05:06

Initial view with activated O2 control and deactivated

pressure alarm (sample values).

CB (E6) 09/2013 Page 46/144

Fixed value

..\ Humidity control Low/Medium/High

Humidity control Medium

Fixed value

..\ Humidity control

Low

Medium

High

6.4 Humidity control of the Permadry™ system

The Permadry™ system with its 2-pan water system guarantees a maximum humidity of up to 95 % r.H. in the inner chamber, which remains condensation-free. This performance assumes an average ambient temperature of 22 ±3 °C / 71.6 ±5.4 °F and a working temperature in the inner chamber of 37 °C /

98.6 °F. You can increase or decrease humidity slightly if required. To configure the humidity control of the Permadry™ system, go to Menu > Humidity control

08.03.2013 05:05:06

Submenu “Humidity control”. The current setting is displayed. To change the setting, press the operating button.

Factory default setting: “Medium”.

Close Home

08.03.2013 05:05:06

Close Home

If the ambient temperature deviates by more than +/- 5 °C from the values recommended by the manufacturer, the conditions for maximum air humidity with condensation-free inner chamber are no longer guaranteed. Contact BINDER Service for assistance.

Submenu “Humidity control”. Select the desired setting

and press the operating button

CB (E6) 09/2013 Page 47/144

Fixed value

..\ Interior socket

Socket On

Socket Off

6.5 Turning on / off the interior socket voltage (with optional interior socket)

For chambers equipped with the optional interior socket (chap. 15.4) you can turn on and off the voltage of the interior socket.

To turn on or off the interior socket voltage, go to Menu > Interior socket (optional)

08.03.2013 05:05:06

Submenu “Interior socket”. Select the desired setting

and press the operating button “Socket On” = socket with activated voltage

“Socket Off” = socket voltage-free

Close Home

Go back to the initial view with “Home”.

This symbol on the controller display indicates that the interior socket is activated.

6.6 Performance during and after power failure

During a power failure, all controller functions are shut down. The gas inlet valves are closed so that no gas can escape into the ambient air. The zero-voltage relay alarm output (9) (chap. 11.6) is switched to alarm position for the whole duration of the power failure.

After the power returns, all functions return to the same status the chamber had before power failure. . The controller continues to function in the original operating mode it was in previously before the power failure occurred. In “fixed value” mode, the setpoints are immediately resumed. The power failure and power return are noted in the event list.

If the CO unit operates in “fixed value” operation mode with the setpoints already entered. All setpoint values remain in memory.

If the temperature or concentration of CO limits during power failure, confirm the alarms with the RESET button as soon as the correct values are reached again (chap. 11.4).

incubator had been running in sterilization mode, the sterilization process is cancelled and the

, and O2 (unit with O2 control) have dropped below the alarm

CB (E6) 09/2013 Page 48/144

Fixed value

..\ System information

Chamber type: CB

Chamber name: CB_O2_E6

Serial number: 13-00000

Special application number: 11-564

Date of production (YY/MM/DD): 2013/01/01

Parameter version: 511B-0004-0015

Firmware version (1): 521C-0001-0023

Firmware version (2): 521B-0005-0017

Fixed value

..\ Service contact

Service hotline

International:

+49 7462 2005 555

USA Toll Free:

+ 1 866 885 9794

+ 1 631 224 4340

CIS:

+ 7 495 988 1517

service@binder-world.com

www.binder-world.com

6.7 Information

You access chamber information like the chamber type, serial no., firmware version etc. To display the system information, go to Menu > System information

08.03.2013 05:05:06

Submenu “System information” (sample values).

Close Home

To display the BINDER Service contact data, go to Menu > Service contact

08.03.2013 05:05:06

Submenu “Service contact”.

Close Home

Further information window can be accessed under Menu > Settings > Network settings > Show

network settings (chap. 9.9) and – for Service purpose – under Menu > Settings > Chamber configuration (chap. 9.12).

CB (E6) 09/2013 Page 49/144

7. Setpoint entry

7.1 Setting ranges

Temperature 7 °C / 12.6 °F above ambient up to 60 °C / 140 °F

CO2

O2 (unit with O2 control)

0 vol.-% up to 20 vol.- %

0.2 vol.-% up to 95 vol.-%

When changing the temperature setpoint, check the setting of the overtemperature safety controller class 3.1 (chap. 14.2).

With setpoint type “Limit”, adapt the safety controller always when changing the temperature setpoint.

When setting a lower temperature setpoint, in order to save time, we recommend cooling down the unit by turning it off and opening both doors of the unit.

When setting a lower CO of the unit for this purpose.

setpoint, the CO2 gas must be able to escape first. Open both doors

7.2 Note when setting high gas concentrations

Notes on handling carbon dioxide (CO2)

Carbon dioxide (CO and therefore practically imperceptible. Vent out any CO or a suitable connection to an exhaust system. We recommend installing a CO

) in high concentrations is hazardous to health. It is colorless and almost odorless

gas that may escape via good room ventilation

warning system.

WARNING

High concentration of CO2 (> 4 Vol.-%). Danger of death by suffocation. Danger of poisoning.

∅ Do NOT set up units in non-ventilated recesses.

 Ensure technical ventilation measures.  Observe the relevant regulations for handling CO

If CO2 is released, leave the area und inform the security service or fire department.

Unit with O

Oxygen (O

control: Notes on handling oxygen (O2)

) is colorless and almost odorless and therefore practically imperceptible. It promotes burns,

which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes and hair. O

is heavier than air and may accumulate in low-lying areas.

CB (E6) 09/2013 Page 50/144

WARNING

High concentration of O2 (> 21 % O2). Fire and explosion hazard through contact of combustible materials with O

Risk of burns and other injuries.

∅ Do NOT set up units in non-ventilated recesses

 Ensure technical ventilation measures  Observe the relevant regulations for handling O

Take appropriate measures to prevent oxygen enrichment and fire and explosion hazards in areas where oxygen enrichment is possible.

Observe the general information for safe handling of oxygen (chap. 1.6).

Unit with O

Nitrogen (N therefore practically imperceptible. Any N

control: Notes on handling nitrogen (N2)

) in high concentrations is hazardous to health. It is colorless and almost odorless and

gas that may escape must be safely led out via good room

ventilation or a suitable connection to an exhaust system.

High concentration of N2. Risk of death by suffocation.

∅ Do NOT set up units in non-ventilated recesses

 Ensure technical ventilation measures  Observe the relevant regulations for handling N

WARNING

CB (E6) 09/2013 Page 51/144

Fixed value

..\ Quick menu

Measurement chart

Active alarms

Temperature setpoint

CO2 setpoint

O2 setpoint

Safety controller setpoint

Hot-air sterilization

[°C]

7.3 Entering the setpoints via “quick menu”

To enter setpoints via quick menu, go to Quick menu.

08.03.2013 05:05:06

“Quick menu”. (item “O2 setpoint” only coming with

control)

Close Home

When trying to enter a setpoint in the „Controller off“ operating mode, a notification window shows “Controller mode is OFF!”. Press the operating button to confirm with “Ok” and change the operating mode to “Fixed value” (chap. 6.2.1).

unit with O Select the desired parameter

and press the operating button.

Temperature setting

To enter the temperature setpoint, go to Quick menu > Temperature setpoint

Fixed value

08.03.2013 05:05:06

..\ Temperature setpoint

Entry menu “Temperature setpoint”.

End Ok 0 1 2 4 5 6 7 8 9 Ins

Select each number with the operating button and press the operating button to confirm.

Setting range: 20 °C / 68 °F up to 60 °C / 140 °F. Press the “Ok” button to confirm.

Close Ok Home

When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.

Go back to the initial view with “Home” or enter the CO

concentration.

CB (E6) 09/2013 Page 52/144

Fixed value

..\ CO2 setpoint

[Vol.-%]

Fixed value

..\ O2 setpoint

[Vol.-%]

Setting the CO

To enter the CO

concentration

setpoint, go to Quick menu > CO2 setpoint

08.03.2013 05:05:06

Entry menu “CO2 setpoint”.

End Ok 0 1 2 3 4

6 7 8 9 Ins Del

Select each number with the operating button and press the operating button to confirm.

Setting range: 0 vol.-% up to 20 vol.-%. Press the “Ok” button to confirm.

Close Ok Home

When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.

Go back to the initial view with “Home” or enter the O

concentration (unit with O2 control).

Setting the O

To enter the O

concentration (unit with O2 control)

setpoint, go to Quick menu > O2 setpoint

08.03.2013 05:05:06

Entry menu “O2 setpoint”.

End Ok 0 1 2 3 4

6 7 8 9 Ins Del

Select each number with the operating button and press the operating button to confirm.

Setting range: 0.2 vol.-% up to 95 vol.-%. Press the “Ok” button to confirm.

Close Ok Home

When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.

Go back to the initial view with “Home”.

CB (E6) 09/2013 Page 53/144

Fixed value

..\ Setpoints

Temperature setpoint

CO2 setpoint

O2 setpoint

Fixed value

..\ Temperature setpoint

[°C]

7.4 Entering the setpoints via general menu

To enter setpoints via general menu, go to Menu > Setpoints

08.03.2013 05:05:06

Submenu “Setpoints”.

Close Home

(item “O2 setpoint” only coming with unit with O control)

Select the desired parameter and press the operating button.

Temperature setting

To enter the temperature setpoint, go to Menu > Setpoints > Temperature setpoint

08.03.2013 05:05:06

Entry menu “Temperature setpoint”.

End Ok 0 1 2 4 5 6 7 8 9 Ins

Select each number with the operating button and press the operating button to confirm.

Setting range: 20 °C / 68 °F up to 60 °C / 140 °F. Press the “Ok” button to confirm.

Close Ok Home

When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.

Go back to the initial view with “Home” or enter the CO

concentration.

CB (E6) 09/2013 Page 54/144

Fixed value

..\ CO2 setpoint

[Vol.-%]

Setting the CO

To enter the CO

concentration

setpoint, go to Menu > Setpoints > CO2 setpoint

08.03.2013 05:05:06

Entry menu “CO2 setpoint”.

End Ok 0 1 2 3 4

6 7 8 9 Ins Del

Select each number with the operating button and press the operating button to confirm.

Setting range: 0 vol.-% up to 20 vol.-%. Press the “Ok” button to confirm.

Close Ok Home

When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.

Go back to the initial view with “Home” or enter the O

concentration (unit with O2 control).

Setting the O

To enter the O

Fixed value

concentration (unit with O2 control)

setpoint, go to Menu > Setpoints > O2 setpoint

08.03.2013 05:05:06

..\ O2 setpoint

Entry menu “O2 setpoint”.

Pos1 End Ok 0 1

3 4 5 6 7 8 9

Select each number with the operating button and press the operating button to confirm.

Setting range: 0.2 vol.-% up to 95 vol.-%. Press the “Ok” button to confirm.

Close Ok Home

When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.

Go back to the initial view with “Home”.

CB (E6) 09/2013 Page 55/144

Fixed value

..\ User

Key lock

Show event list

Home

Fixed value

..\ Key lock

Key lock On

Automatic key lock

Password

8. Key lock

The key lock function serves to block the access to the controller. When the “key lock” function is activated, the controller remains in the actual view and can only be changed when entering the current password.

To configure the key lock function, go to User > Key lock

08.03.2013 05:05:06

“User” menu. Select “Key lock”

and press the operating button.

08.03.2013 05:05:06

Submenu “Key lock”. Select the desired function

and press the operating button.

Close Home

Key lock On The key lock is directly activated

Automatic key lock The key lock is activated automatically after a defined waiting time

Password Change password for unlocking. Factory setting: 0000

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Fixed value

..\ Key lock

Key lock On

Automatic key lock

Password

Fixed value

..\ Automatic key lock

Automatic key lock

Waiting time [min]

8.1 Directly activating the key lock

To directly activate the key lock, go to User > Key lock > Key lock On

08.03.2013 05:05:06

Submenu “Key lock”. Select “Key lock On” to activate the key lock function

immediately and press the operating button.

Close Home

This symbol on the controller displays indicates that the “key lock” function is activated.

The controller remains in the initial view and may be operated only after entering the current password.

Enter password

08.03.2013 05:05:06

..\ Key lock password

Ok: A B C D E F

1 2 3 4 5 6 7 8 9

Entry menu “Key lock password”. Enter the password with the operating button.

Factory setting is 0000 Press the “Ok” button to confirm.

Close Ok Home

8.2 Automatic key lock

To configure the automatic key lock, go to User > Key lock > Automatic key lock

08.03.2013 05:05:06

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Submenu “Automatic Key lock”. Select the desired function

and press the operating button.

Close Home

Fixed value

..\ Automatic key lock On/Off

Automatic key lock : Off

Fixed value

Setpoint

Actual value

37.0

37.2

5.0

85.5

Under “Waiting time [min]” you can enter the waiting time, after which the key lock will be automatically activated. This time starts running off after the last entry to the controller. To enter it, go to

User > Key lock > Automatic key lock > Waiting time [min]

Enter wait time [min]

..\ User\Waiting time

Pos1 End Ok 0

Close Ok Home

To activate the automatic key lock function with the pre-configured waiting time, select

User > Key lock > Automatic key lock > Automatic key lock

08.03.2013 05:05:06

2 3 4 5 6 7 8 9

08.03.2013 05:05:06

Entry menu “Waiting time”. Enter the desired interval with the operating button.

This interval starts running off after the last action on the controller. If the automatic key lock function is enabled, it will become active after this time.

Factory setting: 1 minute. Press the “Ok” button to confirm.

Submenu “Automatic Key lock On/Off”. The current setting is displayed. To change the setting, press the operating button.

“Automatic Key lock : On” = Automatic key lock function activated. The time set under “Waiting time” begins running off.

“Automatic Key lock : Off” = Automatic key lock function deactivated

Close Home

Now the waiting time starts running off. Go back to the initial view with “Home”.

08.03.2013 05:05:06

Temperature [°C]

CO2 [Vol.-%]

Humidity [%rH]

User Quick menu

The controller remains in the initial view and may be operated only after entering the current password. After further entries to the controller, the waiting period begins running again, since the automatic keylock

function remains active until turning it off manually.

Initial view. As soon as the waiting period has expired, the “key

lock” symbol is displayed.

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Fixed value

..\ Key lock

Key lock On

Automatic key lock

Password

Fixed value

..\ Key lock \ Change password

Do not change

Change password

This symbol on the controller displays indicates that the “key lock” function is activated.

8.3 Changing the password for unlocking the key lock

To change the password for unlocking the key lock, go to User > Key lock > Password

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Submenu “Key lock”. Select „Password“

and press the operating button.

Close Home

A security question is displayed:

08.03.2013 05:05:06

Close Home

Change password

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..\ Key lock password

Ok: A B C D E F

1 2 3 4 5 6 7 8 9

Close Ok Home

Submenu “Change password”. To change the password,

select “Change password” and press the operating button.

Entry menu “Key lock password”. Enter the desired password with the operating

button. Factory setting is 0000 Press the “Ok” button to confirm.

Keep well in mind any password modification. Without the correct password, unlocking the key lock is impossible.

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Fixed value

..\ Settings

Setup wizard

Date and time

Sprache, Language, Langue, Idioma, Lingua

Display brightness

Temperature unit

Recording rate

Factory reset

Network settings

Fixed value

..\ Settings

MAC address

Chamber configuration

RS 422 address (optional)

9. General controller settings

In the "Settings" submenu, you can enter the date and time, select the language for the menus and the desired temperature unit, perform the configuration for the controller’s communication functions, and reset the controller to factory settings.

To access the "Settings" submenu, go to Menu > Settings

08.03.2013 05:05:06

Submenu “Settings”. Turn the operating button to see additional menu

items.

Close Home

08.03.2013 05:05:06

Submenu “Settings” (next page).

Close Home

Setup wizard Chap. 9.1

Date and time Setting date and time, chap. 9.2

Sprache, Language, Langue, Idioma, Lingua

Selecting the controller’s menu language, chap. 9.3

Display brightness Adjusting display brightness by turning the operating button

Temperature unit Selecting the temperature unit, chap.9.5

Recording rate Defining the recording rate for data storage, chap. 9.6

Factory reset Factory reset, chap. 9.7

Network settings Network configuration, chap. 9.8

MAC address Displaying the MAC address, chap. 9.10

Chamber configuration

RS 422 address (optional)

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Menu for Service purpose – Entering chamber data like the serial no., service information

Setting the RS 422 unit address(with option RS422 interface), chap.

9.11

Fixed value

..\ Date and time

Set date

Set time

9.1 Setup wizard

The setup wizard will guide you sequentially through the important menus to configure your chamber

• Menu language

• Device name

• Date and time

• IP address

• Subnet mask

• Network name

• Gateway

• DNS 1

• DNS 2

Then the controller returns to the initial view.

Use the setup wizard only if you want to enter all the requested information, as no item can be skipped.

You can configure the network settings (IP address, and the following) only if the DHCP status is Off, otherwise the DHCP server would assign the network configuration.

If you try configuring any network settings (i.e., when reaching item “IP address” in the setup wizard) while DHCP is enabled, the message "DHCP enabled!” is shown. After confirming with Ok the Setup wizard is cancelled and the controller returns to the initial display. Any settings made so far remain valid.

9.2 Date and time settings

To access the date and time settings, go to Menu > Settings > Date and time

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Submenu “Date and time”. Select the desired function

and press the operating button.

Close Home

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Fixed value

8.03.2013

5:05:06

1 2 3 4 5 6 7 8 9

Function “Set date”

08.03.2013 05:05:06

..\ Select date (DD.MM.YYYY)

Ins Pos1 End Ok

1 2 3 4 5 6 7 8 9

Entry menu “Select date”. The current date is shown. If it is incorrect, enter the

correct date with the operating button. Press the “Ok” button to confirm.

Close Ok Home

Function “Set time”

Fixed value

08.03.2013 05:05:06

..\ Set time (HH:MM:SS)

Ins Pos1 End Ok

Entry menu “Set time”. The current time is shown. If it is incorrect, enter the

correct time with the operating button. Press the “Ok” button to confirm.

Close Ok Home

There is no automatic switch to local light-saving times because this could lead to problems with data seeming to be missing or being overwritten in the data base.

Go back to the “Settings” menu with “Close” or to the initial view with “Home”.

CB (E6) 09/2013 Page 62/144

Fixed value

..\ Sprache, Language, Langue, Idioma, Lingua

Deutsch

English

Français

Español

Italiano

Fixed value

..\ Display brightness

9.3 Selecting the menu language of the T4.12 controller

The T4.12 chamber controller communicates via a comprehensible menu navigation in plain text in a selectable language.

To select the desired menu language, go to

Menu > Settings > Sprache, Language, Langue, Idioma, Lingua

08.03.2013 05:05:06

Submenu “Language”. Select the desired language

and press the operating button.

Close Home

Go back to the “Settings” menu with “Close” or to the initial view with “Home”.

9.4 Setting display brightness

To select the display brightness, go to Menu > Settings > Display brightness

08.03.2013 05:05:06

Submenu “Display brightness”. Select the desired setting by turning the

operating button. Confirm by selecting “Save”.

Close Save Home

Go back to the “Settings” menu with “Close” or confirm the change with “Save”: The controller returns to the initial view.

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Fixed value

..\ Temperature unit

Celsius [°C]

Fahrenheit [°F]

Kelvin [K]

9.5 Changing the temperature unit

To select the temperature unit, go to Menu > Settings > Temperature unit

08.03.2013 05:05:06

Submenu “Temperature unit”. Select the desired temperature unit”

and press the operating button.

Close Home

Go back to the “Settings” menu with “Close” or to the initial view with “Home”.

9.6 Defining the data recording rate

To define the recording rate for data storage on the SD card, go to Menu > Settings > Recording rate

Fixed value

..\ Recording rate (seconds)

Ok 0 1 2 3 4 5

Close Ok Home

Go back to the “Settings” menu with “Close” or to the initial view with “Home”.

Note:

08.03.2013 05:05:06

7 8 9 . – Del Ins

Entry menu “Recording rate”. The current recording rate is shown. Enter the

desired value with the operating button. Shortest possible value: 60 seconds. Press the “Ok” button to confirm.

After a period of 3 years, the controller starts overwriting the oldest values on the storage medium. This is independent of the selected storage interval and the actual operating time of the chamber. In any case, the data can be read out at any time using the function “Export to USB drive” (chap. 10.1) and stored externally.

CB (E6) 09/2013 Page 64/144

Fixed value

..\ Factory reset

Do not reset

Really reset

Fixed value

..\ Network settings

Show network settings

DHCP On/Off

IP address

Subnet mask

Chamber name

Network name

Gateway

DNS1

9.7 Factory reset

The “factory reset” function allows resetting the controller configuration to the factory settings.

Risk of data loss! When resetting to factory settings, all controller settings will be deleted. The

event list and stored measuring data are NOT affected.

To access the “Factory reset” function, go to Menu > Settings > Factory reset

08.03.2013 05:05:06

Submenu “Factory reset”. This a security question. Select the desired function

and press the operating button.

Close Home

Go back to the “Settings” menu with “Close” or to the initial view with “Home”.

9.8 Network configuration

The settings of this submenu are required for networking chambers with an Ethernet interface, e.g. to connect them with BINDER’s communication software APT-COM™ 3 DataControlSystem.

You can display the chamber‘s IP address that has been assigned by your DHCP server or manually assign the IP address. All necessary configurations for networking the chamber are available in this menu.

To view and configure the network settings, go to Menu > Settings > Network settings

To configure the network settings, the DHCP state must be set to off. Otherwise, the DHCPserver would assign the network configuration.

If you try to configure the network settings with enabled DHCP state, the message “DHCP enabled!” is shown. Confirm with „Ok“ to return to the “Network settings” menu.

08.03.2013 05:05:06

Close Home

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Submenu “Network settings”. Turn the operating button to see additional menu

items.

Fixed value

..\ Network settings

DNS2

Fixed value

..\ DHCP On/Off

DHCP : On

08.03.2013 05:05:06

Submenu “Network settings” (next page).

Close Home

Show network settings Overview of the entire network configuration

DHCP On/Off

IP address

Subnet mask

Chamber name

Network name

Gateway

DNS1

Switching on and off the DHCP state

Entering the desired IP address

Entering the subnet mask number

Entering the name of the CO

Entering the network name

Entering the gateway number

Entering the DNS1 number

DNS2 Entering the DNS2 number

Set the DHCP State on/off:

22.08.2012 05:05:06

Submenu “DHCP On/Off”. The current DHCP state is displayed. Press the

operating button to change it. Then the new DHCP state is displayed “DHCP : On” = DHCP state activated

“DHCP : Off” = DHCP state deactivated

incubator

Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.

CB (E6) 09/2013 Page 66/144

Close Home

You can only configure the following network settings if the DHCP state is set to “Off”.

92.168.0.100

Fixed value

..\ Subnet mask (n.n.n.n.)

55.255.255.0

Fixed value

..\ Chamber name

B_E6

Enter the IP address:

Fixed value

08.03.2013 05:05:06

..\ IP address (n.n.n.n.)

Entry menu “IP address”

Ins Pos1 End Ok 0

2 3 4 5 6 7 8 9 .

(example value) Enter the desired IP address with the operating

button. Press the “Ok” button to confirm.

Close Ok Home

Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.

Enter the subnet mask:

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Entry menu “Subnet mask”

Pos1 End Ok 0 1

3 4 5 6 7 8 9 , -

(example value) Enter the desired net mask with the operating

button. Press the “Ok” button to confirm.

Close Ok Home

Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.

Enter the chamber name:

08.03.2013 05:05:06

End Ok _ : A B

D E F G H I J K

Entry menu “Chamber name”. Enter the desired chamber name with the operating

button. Press the “Ok” button to confirm.

Close Ok Home

Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.

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B_E6

Fixed value

..\ Gateway (n.n.n.n.)

92.168.0.1

Fixed value

..\ DNS 1 (n.n.n.n.)

92.168.0.1

Enter the network name:

Fixed value

08.03.2013 05:05:06

..\ Network name

End Ok _ : A B

D E F G H I J K

Entry menu “Network name”. Enter the desired network name with the operating

button. Press the “Ok” button to confirm.

Close Ok Home

Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.

Enter the default gateway:

08.03.2013 05:05:06

Pos1 End Ok 0

2 3 4 5 6 7 8 9 ,

Entry menu “Gateway” (example value)

Enter the desired gateway with the operating button. Press the “Ok” button to confirm.

Close Ok Home

Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.

Enter the DNS 1 or DNS 2:

08.03.2013 05:05:06

Ins Pos1 End Ok 0

2 3 4 5 6 7 8 9 ,

Entry menu “DNS 1” or “DNS 2” (example value)

Enter the desired number with the operating button. Press the “Ok” button to confirm.

Close Ok Home

Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.

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Fixed value

..\ Show network settings

DHCP

Off

IP address

192.168.0.100

Net mask

255.255.255.0

Gateway

192.168.0.1

DNS1

192.168.0.1

DNS2

0.0.0.0

Chamber name

CB_E6

BIOS name

CB_E6

00-04-A3-B1-00-B9

9.9 Display of the network configuration

To access the overview of the complete network configuration, go to

Menu > Settings > Network settings > Show network settings

08.03.2013 05:05:06

Overview of the network configuration (sample values)

Home

Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.

9.10 MAC Address

To identify the chamber in the Ethernet network you can display the chamber’s MAC address. To display the chamber’s MAC address, go to Menu > Settings > MAC address

Fixed value

..\ MAC address

Close Home

Go back to the “Settings” menu with “Close” or to the initial view with “Home”.

08.03.2013 05:05:06

Submenu “MAC address” (example value)

The MAC address is displayed.

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Fixed value

..\ RS 422 address

9.11 RS 422 address (with optional RS 422 interface)

For chambers equipped with the optional RS 422 interface, the RS 422 address serves to identify the chamber in a network and to establish communication with the optional BINDER communication software APT-COM™ 3 DataControlSystem. The factory default setting is “1”.

To enter the RS 422 unit address, go to Menu > Settings > RS 422 address (optional)

08.03.2013 05:05:06

Pos1 End Ok 0

2 3 4 5 6 7 8 9

Close Ok Home

Entry menu “RS 422 address”. Enter the desired address (1 up to 254) with the

operating button. Press the “Ok” button to confirm.

Go back to the “Settings” menu with “Close” or to the initial view with “Home”.

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Fixed value

..\ Chamber configuration

Enter serial number

Enter special application number

Service window 1

Service window 2

Fan speed setpoint

Door heating offset

Humidity control offset

Error memory

Fixed value

..\ Chamber configuration

Firmware update

9.12 Display and entry of the device configuration – for service purpose

Information about the chamber, such as chamber type, name, serial number, firmware version, etc. can be viewed under Menu > System information (chap. 6.7).

To access the device configuration menu, go to Menu > Settings > Chamber configuration This menu is password protected and only intended for Service purpose.

08.03.2013 05:05:06

Submenu “Chamber configuration”.

Close Home

Turn the operating button to see additional menu items.

08.03.2013 05:05:06

Submenu “Chamber configuration” (next page).

Close Home

After selecting the desired function, the service password is requested in order to deny unauthorized access.

CB (E6) 09/2013 Page 71/144

Fixed value

..\ Import/Export to USB drive

Export to USB drive

Import from USB drive

Fixed value

..\ Export to USB drive

Chamber status

Measurement values

Event list

Configuration

Adjustment

Program parameters

System information

All

10. Data transfer via USB interface

The USB port is located in the instrument box. To access the submenus for data transfer, go to Menu > Import/Export

08.03.2013 05:05:06

Submenu “Import/Export to USB drive”. Select the desired type of communication via USB

port (data export or import) and press the operating button.

Close Home

It is possible that some USB devices are not recognized due to compatibility issues. In this case, please use a USB storage device from a different manufacturer.

10.1 Exporting data to USB drive

Insert the USB stick or the plug of your USB drive into the USB port in the instrument box. To configure data export to USB media, go to Menu > Import/Export > Export to USB drive

08.03.2013 05:05:06

Submenu “Export to USB drive”.

Close Home

Select the desired data type and press the operating button. Data is written to the connected media.

Chamber status Actual chamber status, including operating mode, setpoints etc.

Measurement values Measured data

Event list List of status information and errors (see chap. 12)

Configuration (Service only)

Adjustment Adjustment data

Program parameters (Service only)

System information (Service only)

All All data,

CB (E6) 09/2013 Page 72/144

Fixed value

..\ Import from USB drive

Configuration

If no USB device has been connected, the message “Insert USB device” is displayed. It disappears after inserting the USB stick or the plug of your USB drive into the USB port in the instrument box.

This symbol on the controller displays indicates that data are being transmitted via the USB port.

10.2 Importing data from USB drive

Insert the USB stick or the plug of your USB drive into the USB port in the instrument box. To configure data import from USB media, go to Menu > Import/Export > Import from USB drive

08.03.2013 05:05:06

Close Home

Configuration

(Service only)

This symbol on the controller displays indicates that data are being transmitted via the USB port.

Submenu “Import from USB drive”. Select “Configuration”

and press the operating button. Data is read from the connected media.

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Icon

Signification

Icon

Signification

Icon

Notifying message

Signification

Icon

Alarm message

Signification

11. Notifications and Alarms

11.1 Notifications overview

control deactivated. No O2

Fixed value operation

2/N2

concentration alarms will occur. (unit with O

control)

Heating active

Key lock activated

Copying data via USB

Interior socket turned on (with option interior socket)

CO2 pressure alarm deactivated

O2 pressure alarm deactivated (unit with O

control)

N2 pressure alarm deactivated (unit with O

control)

DO NOT OPEN THE DOOR !

STERILIZATION FINISHED

STERILIZATION ABORTED

Hot air sterilization running

Hot air sterilization successful The inner chamber and parts inside can still be hot. Do not touch.

Hot air sterilization cancelled

The inner chamber and parts inside can still be hot. Do not touch.

11.2 Alarm messages overview

Safety controller overtemperature

Temp. range

CO2 range

O2 range

Overtemperature safety controller alarm (class 3.1): selected value of the safety controller exceeded.

Having reached the setpoint, the temperature deviates by more than the set tolerance range value (chap. 11.5.6) and longer than 10 min. from the setpoint or the temperature doesn’t reach the tolerance range within 3 hours from turning on the unit or closing the door

Having reached the setpoint, the CO

concentration deviates by more than

the set tolerance range value (chap. 11.5.6) and longer than 10 min. from the setpoint or the CO

concentration doesn’t reach the tolerance range

within 3 hours from turning on the unit or closing the door

Having reached the setpoint, the O

concentration deviates by more than

the set tolerance range value (chap. 11.5.6) and longer than 10 min. from the setpoint or the O within 3 hours from turning on the unit or closing the door (unit with O

concentration doesn’t reach the tolerance range

control)

CB (E6) 09/2013 Page 74/144

Icon

Alarm message

Signification

After the humidity was situated within the defined tolerance range (chap.

Humidity range

11.5.6) it deviates longer than 10 min. from the tolerance range or the humidity doesn’t reach the tolerance range within 3 hours from turning on the unit or closing the door

Door open

Door is open for more than the set alarm delay time (chap. 11.5.5, factory setting: 1 minute). Close the door.

Low pressure CO2 Low CO2 outlet pressure (< 0.3 bar)

Low pressure CO2 Low O2 outlet pressure (< 0.3 bar) (unit with O2 control)

Low pressure N2 Low N2 outlet pressure (< 0.3 bar) (unit with O2 control)

CO2 sensor defective

O2 sensor defective

Inform service adjust CO2 sensor

sensor defective. Contact BINDER Service.

sensor adjustment required. Contact BINDER Service.

You can activate / deactivate the buzzer in the “Alarms” submenu (chap. 11.5.3). With an activated buzzer there is an audible alert with an alarm. You can reset it in the “Alarms”

submenu for alarm acknowledgement pressing the “Reset” button (chap. 11.4). The alarm symbol will only disappear when the cause of the alarm has been remedied.

The zero-voltage relay alarm contact (chap. 11.6) is activated in case of the alarm messages “door open” and deviations of temperature and gas concentrations) as well as in case of a power failure and turning off the incubator at its main power switch.

For appropriate actions in the event of an alarm, please refer to chap. 21 “Troubleshooting”.

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11.3 Alarm status

An alarm message can appear in 3 different states:

“Set”

• Active alarm.

• The corresponding alarm icon is displayed in the initial view The buzzer sounds (if activated).

• The “Info” button in the initial view leads to the “Alarms” submenu for alarm acknowledgement.

• Press the “Reset” button in the “Alarms” submenu for alarm acknowledgement to mute the buzzer and

confirm the alarm.

“Acknowledged”

• Active alarm.

• The alarm was acknowledged. The buzzer is off.

• The alarm cause is still valid. Therefore, the alarm icon remains displayed in the initial view.

• The alarm message figures in the list of active alarms.

“Cleared”

• The alarm cause has disappeared.

• The alarm icon is not displayed any longer

• The alarm message has disappeared from the list of active alarms.

• The alarm message remains in the “Event list” for information.

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Fixed value

Setpoint

Actual value

37.0

39.2

5.0

85.5

Info

Alarm acknowledgement

../Home/Alarms

Safety controller overtemperature

11.4 Confirming an active “set” alarm

08.03.2013 05:05:06

Temperature [°C]

CO2 [Vol.-%]

Humidity [%]

User

08.03.2013 05:05:06

Initial view with overtemperature safety controller alarm.

The buzzer sounds (if not deactivated previously). Press the “Info” button.

Submenu “Alarms” for alarm acknowledgement.

Close Reset Home

Press the “Reset” button to confirm the alarm. If there is another active “set” alarm, you can also confirm it with the “Reset” button. After confirming all active “set” alarms, the buzzer is off, and the initial view is displayed.

As long as an alarm is still active, i.e. as long as the alarm cause is still valid, the alarm icon continues to be displayed in the initial view.

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Fixed value

..\ Alarm

Active alarms

History

Buzzer test

Buzzer On/Off

Alarms On/Off

Door alarm delay

Tolerance ranges

Fixed value

..\ Active alarms

Safety controller overtemperature

11.5 Alarm configuration and overview

To access the alarm lists and configuration menu, go to Menu > Alarms

08.03.2013 05:05:06

Submenu “Alarm”. Select the desired function

and press the operating button.

Close Home

Active Alarms List of the alarms with status “set” or “confirmed”.

History List of all alarms (status “set” or “confirmed ” or “cleared”)

Buzzer test Testing the alarm buzzer, chap. 11.5.3

Buzzer On/Off Activating / deactivating the alarm buzzer, chap. 11.5.3

Alarms On/Off

Activating / deactivating the alarm functions. Off: Alarm buzzer off, no alarm icons displayed.

Door alarm delay Entering the delay time of the door alarm

Tolerance ranges

Defining the tolerance ranges and alarm delay times for the individual parameters

11.5.1 List of active alarms

To access the overview list of active alarms, go to Menu > Alarms > Active alarms

08.03.2013 05:05:06

Submenu “Active alarms“. All active alarms with status “set”

are listed.

or “acknowledged”,

Close Home

If no alarm is active (“set” or “acknowledged”), no message will be displayed in this window.

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Fixed value

..\ History

08.03.2013 : Messages of indicated day

14:39:48

Alarm set Safety controller overtemperature

15:03:22

Alarm cleared Safety controller overtemperature

8.03.2013

Fixed value

..\ Event list

08.03.2013 : Messages of indicated day