BINDER CB 60 User Manual

Operating Manual
APT.line™ CB (E6) CO CO
- Incubators
2
– Incubators with O2 control
2
with sterilizable NDIR sensor system for CO2 and humidity and microprocessor controller T4.12
Model Voltage Equipment Art. No.
CB 60 230 V 9040-0088, 9140-0088
CB 60-UL 100-120V 9040-0089, 9140-0089
CB 60 230 V with O2 control 9040-0090, 9140-0090
CB 60-UL 100-120V with O2 control 9040-0091, 9140-0091
CB 160 (E6) 230 V 9040-0092, 9140-0092
CB 160-UL 100-120V 9040-0093, 9140-0093
CB 160 (E6) 230 V with O2 control 9040-0094, 9140-0094
CB 160-UL 100-120V with O2 control 9040-0095, 9140-0095
CB 160 230 V with divided glass door 9040-0100, 9140-0100
CB 160-UL 100-120V with divided glass door 9040-0101, 9140-0101
CB 160 230 V with O2 control with divided glass door 9040-0102, 9140-0102
CB 160-UL 100-120V with O2 control with divided glass door 9040-0103, 9140-0103
CB 220 230 V 9040-0096, 9140-0096
CB 220-UL 100-120V 9040-0097, 9140-0097
CB 220 230 V with O2 control 9040-0098, 9140-0098
CB 220-UL 100-120V with O2 control 9040-0099, 9140-0099
BINDER GmbH
Address Post office box 102 D-78502 Tuttlingen Tel. +49 7462 2005 0 Fax +49 7462 2005 100 Internet http://www.binder-world.com E-mail info@binder-world.com Service Hotline +49 7462 2005 555 Service Fax +49 7462 2005 93 555 Service E-Mail service@binder-world.com Service Hotline USA +1 866 885 9794 or +1 631 224 4340 x3 Service Hotline Asia Pacific +852 39070500 or +852 39070503 Service Hotline Russia and CIS +7 495 98815 17
Issue 09/2013 Art. No. 7001-0229
EG – KONFORMITÄTSERKLÄRUNG
Anschrift / Address / Adresse:
Typenbezeichnung / Type / Type:
employé dans certaines limites de tension

EG – declaration of conformity

EC - DECLARATION OF CONFORMITY CE - DECLARATION DE CONFORMITE
Anbieter / Supplier / Fournisseur:
Produkt / Product / Produit:
Die oben beschriebenen Produkte sind konform mit folgenden EG-Richtlinien: The products described above are in conformity with the following EC guidelines: Les produits décrits ci-dessus sont conformes aux directives CE suivantes:
Niederspannungsrichtlinie 2006/95/EG
Low voltage directive 2006/95/EC
Directive basse tension 2006/95/CE
BINDER GmbH Im Mittleren Ösch 5, D-78532 Tuttlingen CO
Begasungsbrutschrank
2
Incubators
CO
2
Incubateurs à CO CB 60, CB 160, CB 220
Richtlinie 2006/95/EG des Europäischen Parlaments und des Rates vom 12. Dezember 2006 zur Angleichung der Rechtsvorschriften der Mitgliedstaaten betreffend elektrische Betriebsmittel zur Verwendung innerhalb bestimmter Spannungsgrenzen
Council Directive 2006/95/EC of 12 December 2006 on the harmonization of the laws of Member States relating to electrical equipment designed for use within certain voltage limits
Directive 2006/95/CE du Parlement Européen et du Conseil du 12 décembre 2006 concernant le rapprochement des législations des États membres relatives au matériel électrique destiné à être
2
EMV-Richtlinie 2004/108/EG
EMC Directive 2004/108/EC
Directive CEM 2004/108/CE
Die oben beschriebenen Produkte tragen entsprechend die Kennzeichnung CE. The products described above, corresponding to this, bear the CE-mark. Les produits décrits ci-dessus, en correspondance, portent l’indication CE.
CB (E6) 09/2013 Page 2/144
Richtlinie 2004/108/EG des Europäischen Parlaments und des Rates vom 15. Dezember 2004 zur Angleichung der Rechtsvorschriften der Mitgliedstaaten über die elektromagnetische Verträglichkeit und zur Aufhebung der Richtlinie 89/336/EWG.
Directive 2004/108/EC of the European Parliament and of the Council of 15 December 2004 on the approximation of the laws of the Member States relating to electromagnetic compatibility and repealing Directive 98/336/EEC.
Directive 2004/108/CE du Parlement Européen et du Conseil du 15 décembre 2004 relative au rapprochement des législations des États membres concernant la compatibilité électromagnétique et abrogeant le directive 98/336/CEE.
1 / 3
Sicherheit / safety / sécurité:
EMV / EMC / CEM:
Die oben beschriebenen Produkte sind konform mit folgenden harmonisierten Normen: The products described above are in conformity with the following harmonized standards: Les produits décrits ci-dessus sont conformes aux normes harmonisées suivantes:
EN 61010-1:2010 Sicherheitsbestimmungen für elektrische Mess-, Steuer-, Regel- und
Laborgeräte – Teil 1: Allgemeine Anforderungen (DIN EN 61010­1:2011, VDE 411-1:2011)
Safety requirements for electrical equipment for measurement, control, and laboratory use – Part 1: General requirements (IEC 61010-1:2010, BS EN 61010-1:2010)
Règles de sécurité pour appareils électriques de mesurage, de régulation et de laboratoire – Partie 1: Prescriptions générales (CEI 61010-1:2010, NF EN 61010:2011)
EN 61010-2-010:2003 Sicherheitsbestimmungen für elektrische Meß-, Steuer-, Regel- und
Laborgeräte – Teil 2-010: Besondere Anforderungen an Laborgeräte für das Erhitzen von Stoffen (DIN EN 61010-2-010:2004)
Safety requirements for electrical equipment for measurement, control, and laboratory use – Part 2-010: Particular requirements for laboratory equipment for the heating of materials (IEC 61010-2-10:2005, BS EN 61010-2-10:2003)
Règles de sécurité pour appareils électriques de mesurage, de régulation et de laboratoire – Partie 2-010 : Prescriptions particulières pour appareils de laboratoire utilisés pour l’échauffement des matières (CEI 61010-2-10:2003, NF EN 61010-2-10:2005)
EN 61326-1:2006 + Corr. 1:2008 + Corr. 2:2010
Elektrische Mess-, Steuer-, Regel- und Laborgeräte - EMV­Anforderungen - Teil 1: Allgemeine Anforderungen (DIN EN 61326­1:2006 + Berichtigung 1:2008 + Berichtigung 2:2011)
Electrical equipment for measurement, control and laboratory use ­EMC requirements - Part 1: General requirements (IEC 61326-1:2005 + Corr. 1:2008 + Corr. 2:2010, BS EN 61326-1:2006+ A1:2008)
Matériel électrique de mesure, de commande et de laboratoire ­Exigences relatives à la CEM - Partie 1: Exigences générales (CEI 61326-1:2005 + AC1:2008, NF EN 61326-1:2006 mod.)
EN 61326-2-2:2006 Elektrische Mess-, Steuer-, Regel- und Laborgeräte – EMV-
Anforderungen. Teil 2-2: Besondere Anforderungen - Prüfanordnung, Betriebsbedingungen und Leistungsmerkmale für ortsveränderliche Prüf-, Mess- und Überwachungsgeräte in Niederspannungs­Stromversorgungsnetzen. (DIN EN 61326-2-2:2006)
Electrical equipment for measurement, control and laboratory use – EMC requirements. Part 2-2: Particular requirements - Test configurations, operational conditions and performance criteria for portable test, measuring and monitoring equipment used in low-voltage distribution systems. (IEC 61326-2-2:2005, BS EN 61326-2-2:2006)
Matériel électrique de mesure, de commande et de laboratoire – Exigences relatives à la CEM. Partie 2-2: Exigences particulières ­Configurations d’essai, conditions de fonctionnement et critères d’aptitude à la fonction des matériels portatifs d’essai, de mesure et de surveillance utilisés dans des systèmes de distribution basse tension. (CEI 61326-2-2:2005 + AC1:2007, NF EN 61326-2-2:2006)
CB (E6) 09/2013 Page 3/144
2 / 3
P. M. Binder
B. Hofmann
D-78532 Tuttlingen, 20.07.2012 BINDER GmbH
Geschäftsführender Gesellschafter Managing Director Directeur général
Leiter F & E Director R & D Chef de service R&D
3 / 3
CB (E6) 09/2013 Page 4/144

Product registration

CB (E6) 09/2013 Page 5/144
Contents
EG – declaration of conformity ...................................................................................................................... 2
Product registration ....................................................................................................................................... 5
1. SAFETY ................................................................................................................ 10
1.1 Legal considerations ......................................................................................................................... 10
1.2 Structure of the safety instructions .................................................................................................... 10
1.2.1 Signal word panel ................................................................................................................... 10
1.2.2 Safety alert symbol ................................................................................................................. 11
1.2.3 Pictograms .............................................................................................................................. 11
1.2.4 Word message panel structure ............................................................................................... 12
1.3 Localization / position of safety labels on the unit ............................................................................. 12
1.4 Type plate.......................................................................................................................................... 14
1.5 General safety instructions on installing and operating the CO2 incubator....................................... 15
1.6 Precautions when working with gases .............................................................................................. 16
1.7 Precautions when handling gas cylinders ......................................................................................... 18
1.8 Intended use ..................................................................................................................................... 18
2. UNIT DESCRIPTION ............................................................................................ 19
2.1 Unit overview ..................................................................................................................................... 20
2.2 Instrument panel ............................................................................................................................... 20
2.3 Inner chamber ................................................................................................................................... 21
2.4 Control panel on the rear of the unit ................................................................................................. 23
3. COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND
INSTALLATION .................................................................................................... 24
3.1 Unpacking, and checking equipment and completeness of delivery ................................................ 24
3.2 Guidelines for safe lifting and transportation ..................................................................................... 24
3.3 Storage .............................................................................................................................................. 25
3.4 Location of installation and ambient conditions ................................................................................ 25
4. INSTALLATION AND CONNECTIONS ............................................................... 29
4.1 Shelves .............................................................................................................................................. 29
4.2 Permadry™ water pan ...................................................................................................................... 29
4.3 Gas connections ............................................................................................................................... 30
4.3.1 Connection of the CO2 gas cylinder ........................................................................................ 31
4.3.2 Connection of the O2 gas cylinder (unit with O2 control) ......................................................... 32
4.3.3 Connection of the N2 gas cylinder (unit with O2 control) ......................................................... 33
4.3.4 Connecting the gas hose to the unit rear (for CO2, O2, N2) .................................................... 34
4.3.5 Gas cylinder connection kits (option) ...................................................................................... 35
4.4 Electrical connection ......................................................................................................................... 36
4.5 Handling and aligning the multiple-divided inner glass door, gas proof (optional equipment) ........ 37
5. START UP ............................................................................................................ 38
5.1 Equilibration time ............................................................................................................................... 38
5.2 Factory settings ................................................................................................................................. 38
6. FUNCTIONAL OVERVIEW OF THE T4.12 CHAMBER CONTROLLER ............. 39
6.1 Menu structure .................................................................................................................................. 40
6.1.1 General menu ......................................................................................................................... 40
6.1.2 Quick menu ............................................................................................................................. 41
6.1.3 User menu .............................................................................................................................. 42
6.2 Operating modes ............................................................................................................................... 43
6.2.1 Activating the “control offmode or change to “fixed value” operating mode ......................... 43
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Deactivating the O
6.3
6.3.1 Required gas supply of the unit with O2 control ...................................................................... 45
6.3.2 Activating / deactivating the O2 control and O2 / N2 pressure alarms ..................................... 46
6.4 Humidity control of the Permadry™ system ...................................................................................... 47
6.5 Turning on / off the interior socket voltage (with optional interior socket) ......................................... 48
6.6 Performance during and after power failure ...................................................................................... 48
6.7 Information ........................................................................................................................................ 49
control and O2/N2 pressure alarms (unit with O2 control) .................................. 45
2
7. SETPOINT ENTRY ............................................................................................... 50
7.1 Setting ranges ................................................................................................................................... 50
7.2 Note when setting high gas concentrations ...................................................................................... 50
7.3 Entering the setpoints via “quick menu” ............................................................................................ 52
7.4 Entering the setpoints via general menu ........................................................................................... 54
8. KEY LOCK ........................................................................................................... 56
8.1 Directly activating the key lock .......................................................................................................... 57
8.2 Automatic key lock ............................................................................................................................ 57
8.3 Changing the password for unlocking the key lock ........................................................................... 59
9. GENERAL CONTROLLER SETTINGS ................................................................ 60
9.1 Setup wizard ..................................................................................................................................... 61
9.2 Date and time settings ...................................................................................................................... 61
9.3 Selecting the menu language of the T4.12 controller ....................................................................... 63
9.4 Setting display brightness ................................................................................................................. 63
9.5 Changing the temperature unit.......................................................................................................... 64
9.6 Defining the data recording rate ........................................................................................................ 64
9.7 Factory reset ..................................................................................................................................... 65
9.8 Network configuration ....................................................................................................................... 65
9.9 Display of the network configuration ................................................................................................. 69
9.10 MAC Address .................................................................................................................................... 69
9.11 RS 422 address (with optional RS 422 interface) ............................................................................. 70
9.12 Display and entry of the device configuration – for service purpose ................................................ 71
10. DATA TRANSFER VIA USB INTERFACE........................................................... 72
10.1 Exporting data to USB drive .............................................................................................................. 72
10.2 Importing data from USB drive .......................................................................................................... 73
11. NOTIFICATIONS AND ALARMS ......................................................................... 74
11.1 Notifications overview ....................................................................................................................... 74
11.2 Alarm messages overview ................................................................................................................ 74
11.3 Alarm status ...................................................................................................................................... 76
11.4 Confirming an active “set” alarm ....................................................................................................... 77
11.5 Alarm configuration and overview ..................................................................................................... 78
11.5.1 List of active alarms ................................................................................................................ 78
11.5.2 History – list of all alarms ........................................................................................................ 79
11.5.3 Activating, deactivating, and testing the alarm buzzer ........................................................... 80
11.5.4 Activating / deactivating all alarm functions ............................................................................ 80
11.5.5 Setting the delay time after opening the door ......................................................................... 81
11.5.6 Tolerance ranges and alarm delay times................................................................................ 81
11.6 Zero-voltage relay alarm output ........................................................................................................ 86
12. EVENT LIST ......................................................................................................... 87
13. GRAPHICAL DISPLAY OF THE MEASURED VALUES ..................................... 88
13.1 Setting the sampling rate .................................................................................................................. 88
13.2 Defining the display range................................................................................................................. 89
13.3 Selecting the parameters .................................................................................................................. 90
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14. TEMPERATURE SAFETY DEVICES ................................................................... 91
14.1 Overtemperature protective device (class 1) .................................................................................... 91
14.2 Overtemperature safety controller (temperature safety device class 3.1) ........................................ 91
14.2.1 Safety controller modes .......................................................................................................... 91
14.2.2 Setting the safety controller .................................................................................................... 92
15. OPTIONS .............................................................................................................. 95
15.1 Communication software APT-COM™ 3 DataControlSystem (option) ............................................ 95
15.2 RS 422 interface (option) .................................................................................................................. 95
15.3 Silicone access ports 30 mm / 1.18 in, closable from both sides with silicon plugs (option) ............ 95
15.4 Interior socket 230V (option) ............................................................................................................. 96
15.5 Analog outputs for temperature and CO2 (option) ............................................................................ 98
15.6 Access port for extra-low voltage (option) ......................................................................................... 98
15.7 BINDER Gas Supply Service – External bottle changer for CO2, N2 or O2 (option) ......................... 99
15.8 Stands ............................................................................................................................................. 100
15.8.1 Stacking stand (option) ......................................................................................................... 100
15.8.2 Stacking adapter for direct thermal decoupled stacking (option) ......................................... 100
15.8.3 Base on castors (option) ....................................................................................................... 100
16. REFERENCE MEASUREMENTS ...................................................................... 101
16.1 CO2 reference measuring ............................................................................................................... 101
16.1.1 Measuring CO2 concentration indirectly via the pH of the cell medium ................................ 101
16.1.2 Measuring CO2 directly via chemical indicator tubes............................................................ 102
16.1.3 Measuring CO2 directly with an electronic infrared measuring device ................................. 103
16.2 Temperature reference measurement ............................................................................................ 103
17. AVOIDING MICROBIAL CONTAMINATION...................................................... 103
17.1 Cells and media .............................................................................................................................. 103
17.2 Laboratory conditions / equipment around the incubator ................................................................ 103
17.3 Working and behavior in the lab ..................................................................................................... 104
17.4 Chamber design and equipment of the CO2 incubator ................................................................... 104
17.5 Handling the CO2 incubator ............................................................................................................ 105
18. CLEANING, DECONTAMINATION / DISINFECTION, AND STERILIZATION .. 107
18.1 Cleaning .......................................................................................................................................... 107
18.2 Decontamination / chemical disinfection of the CO2 incubator ....................................................... 108
18.3 Hot-air sterilization at 180 °C / 356 °F ............................................................................................. 110
18.3.1 Overview ............................................................................................................................... 110
18.3.2 Performing a hot-air sterilization ........................................................................................... 111
18.3.3 Aborting the hot-air sterilization prematurely ........................................................................ 113
18.3.4 End of the sterilization cycle ................................................................................................. 115
19. MAINTENANCE AND SERVICE ........................................................................ 116
19.1 Maintenance intervals, service ........................................................................................................ 116
19.2 Checking the air jacket heating fan ................................................................................................. 116
19.3 Checking the humidity system fan .................................................................................................. 117
19.4 Gas inlet fine filter ........................................................................................................................... 117
19.5 Sending the unit back to BINDER GmbH ....................................................................................... 117
20. DISPOSAL.......................................................................................................... 118
20.1 Disposal of the transport packing .................................................................................................... 118
20.1.1 Outer unit packing ................................................................................................................. 118
1.1.1 Packing inside the unit and equipment ................................................................................. 118
20.2 Decommissioning ............................................................................................................................ 119
20.3 Disposal of the unit in the Federal Republic of Germany ............................................................... 119
20.4 Disposal of the unit in the member states of the EC except for the Federal Republic of Germany 120
20.5 Disposal of the unit in non-member states of the EC ..................................................................... 121
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21. TROUBLESHOOTING ....................................................................................... 122
21.1 Heating ............................................................................................................................................ 122
21.2 Gas cylinder pressure too low ......................................................................................................... 123
21.3 Gas concentration ........................................................................................................................... 125
21.4 Sterilization ...................................................................................................................................... 127
21.5 Humidity .......................................................................................................................................... 127
21.6 Controller ......................................................................................................................................... 129
21.7 Open door ....................................................................................................................................... 129
22. TECHNICAL DESCRIPTION .............................................................................. 130
22.1 Factory calibration and adjustment ................................................................................................. 130
22.2 Over current protection ................................................................................................................... 130
22.3 Definition of usable volume ............................................................................................................. 130
22.4 CB technical data ............................................................................................................................ 131
22.5 Equipment and Options ................................................................................................................... 133
22.6 Spare parts and accessories .......................................................................................................... 134
22.7 Important conversion data for non-SI units ..................................................................................... 135
22.8 Conversion table for gas inlet pressures, bar – psi ......................................................................... 135
22.9 Dimensions CB 60 .......................................................................................................................... 136
22.10 Dimensions CB 160 ........................................................................................................................ 137
22.11 Dimensions CB 220 ........................................................................................................................ 138
23. CONTAMINATION CLEARANCE CERTIFICATE ............................................. 139
23.1 For units located outside North America and Central America ....................................................... 139
23.2 For units in North America and Central America ............................................................................ 142
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Dear customer,
For the correct operation of the CO completely and carefully and observe all instructions as indicated. Failure to read, understand and follow the instructions may result in personal injury. It can also lead to damage to the unit and/or poor equipment performance
incubator CB, it is important that you read this operating manual
2

1. Safety

This operating manual is part of the components of delivery. Always keep it handy for reference. The device should only be operated by laboratory personnel especially trained for this purpose and familiar with all precautionary measures required for working in a laboratory. To avoid injuries and damage observe the safety instructions of the operating manual.
WARNING
Failure to observe the safety instructions. Serious injuries and unit damage.
Observe the safety instructions in this operating manual. Carefully read the complete operating instructions of the CO

1.1 Legal considerations

This operating manual is for informational purposes only. It contains information for installing, start-up, operation and maintenance of the product. Note: the contents and the product described are subject to change without notice.
incubator CB.
2
Understanding and observing the instructions in this operating manual are prerequisites for hazard-free use and safety during operation and maintenance. In no event shall BINDER be held liable for any damages, direct or incidental arising out of or related to the use of this manual.
This operating manual cannot cover all conceivable applications. If you would like additional information, or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or contact us directly by phone at the number located on page one of this manual
Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or existing agreement, description, or legal relationship, nor do they modify such a relationship. All obligations on the part of BINDER derive from the respective purchase contract, which also contains the entire and exclusively valid statement of warranty administration. The statements in this manual neither augment nor restrict the contractual warranty provisions.

1.2 Structure of the safety instructions

In this operating manual, the following safety definitions and symbols indicate dangerous situations following the harmonization of ISO 3864-2 and ANSI Z535.6.

1.2.1 Signal word panel

Depending on the probability of serious consequences, potential dangers are identified with a signal word, the corresponding safety color, and if appropriate, the safety alert symbol.
DANGER
Indicates an imminently hazardous situation that, if not avoided, will result in death or serious (irreversible) injury.
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Warning signs
Electrical hazard
Hot surface
Explosive atmosphere
poisoning hazard
or chemical burns
Biohazard
WARNING
Indicates a potentially hazardous situation which, if not avoided, could result in death or serious (irreversible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor (reversible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product and/or its functions or of a property in its proximity.

1.2.2 Safety alert symbol

Use of the safety alert symbol indicates a risk of injury. Observe all measures that are marked with the safety alert symbol in order to avoid death or
injury.

1.2.3 Pictograms

Lifting hazard
Explosive substances
Gas cylinders
Fire promoting agents
Pollution Hazard
Suffocation hazard
Harmful substances
Stability hazard
CO2 suffocation and
Risk of corrosion and /
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Mandatory action signs
plug
Environment protection
Wear protective gloves
Prohibition signs
water
Pictograms (Warning signs)
Service label
Mandatory regulation
Do NOT touch
Information to be observed in order to ensure optimum function of the product.
Read operating
instructions
Do NOT spray with

1.2.4 Word message panel structure

Type / cause of hazard. Possible consequences.
Instruction how to avoid the hazard: prohibition Instruction how to avoid the hazard: mandatory action
Disconnect the power
Wear safety goggles
Do NOT climb
Lift with several persons
Observe all other notes and information not necessarily emphasized in the same way, in order to avoid disruptions that could result in direct or indirect injury or property damage.

1.3 Localization / position of safety labels on the unit

The following labels are located on the unit:
Hot surface
Risk of injury
on the outer door: CB-UL only
above the access ports (option)
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CO2 -Incubator CB CO2 -Incubator CB-UL
Figure 1: Position of labels on the unit
Keep safety labels complete and legible.
Replace safety labels that are no longer legible. Contact BINDER service for these replacements.
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Indications of the type plate
Information
BINDER
Manufacturer: BINDER GmbH
CB 160
Model CB 160
Serial No.
00-00000
Serial No. 00-00000
Nominal temperature
187 °C 369 °F
Enclosure protection
IP 20
IP type of protection 20 acc. to EN 60529
Temp. safety device
DIN 12880
Temperature safety device acc. to standard DIN 12880
Class
3.1
Temperature safety device, class 3.1
Art. No.
9040-0092
Art. No. 9040-0092
Project No.
---
(Special application acc. to project no.)
1,30 kW
Nominal power 1,30 kW
200-240 V 1 N ~
Nominal voltage 200-240 V ± 10%, single-phase unit
5,7 A
Nominal current 5,7 Amp
50/60 Hz
Power frequency 50/60 Hz
Symbol on the type plate
Information
LABORATORY EQUIPMENT 43KM
Nominal temperature
187 °C
1,30 kW
369°F
200-240 V 1 N ~
Temp. safety device
IP 20 DIN 12880
50/60 Hz
Class
3.1
Art. No.
9040-0092
US PATS 4585923 / 5222612 / 5309981
Project No.
5405194 / 5601143 / 5773287 / 6079403
D 78532 Tuttlingen / Germany
Internet: www.binder-world.com

1.4 Type plate

Position of type plate: left unit side (seen from front), at the bottom in the middle
Enclosure protection
5,7 A
Tel. + 49 (0) 7462/ 2005-0
CB 160 Serial No. 00-00000
Figure 2: Type plate (example of CB 160 regular unit)
Nominal temperature
Made in Germany
CE conformity marking
Electrical and electronic equipment manufactured / placed on the market in the EC after 13 August 2005 and to be disposed of in separate collection according to directive 2002/96/EC on waste electrical and electronic equipment (WEEE).
The equipment is certified in the GOST R certification system of GOSTSTANDARD Russia.
The equipment is certified by Underwriters Laboratories Inc.® according to standards CAN/CSA-C22.2 No. 61010-1, 2
nd
Edition, 2004-07 (Electrical Equipment for Measurement,
(CB-UL only)
Control, and Laboratory Use; Part 1: General Requirements); UL 61010-1, 2 Measurement, Control, and Laboratory Use; Part 1: General Requirements); IEC 61010-1:2010, 3
nd
Edition, 2005-07-22 (Electrical Equipment for
rd
Edition and IEC 61010-2­10:2003 (Particular Requirements for Laboratory Equipment for the heating of materials).
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1.5 General safety instructions on installing and operating the CO2 incubator

With regard to operating the CO2 incubator CB and to the installation location, please observe the guideline BGI/GUV-I 850-0 on safe working in laboratories (formerly BGR/GUV-R 120 or ZH 1/119 laboratory guidelines issued by the employers’ liability insurance association) (for Germany).
BINDER GmbH is only responsible for the safety features of the unit provided skilled electricians or qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating to chamber safety are replaced in the event of failure with original spare parts.
To operate the unit, use only original BINDER accessories or accessories from third-party suppliers authorized by BINDER. The user is responsible for any risk caused by using unauthorized accessories.
CAUTION
Danger of overheating. Damage to the unit.
Do NOT install the unit in unventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
Do not operate the CO2 incubator CB in hazardous locations.
DANGER
Explosion hazard. Danger of death.
Do NOT operate the unit in potentially explosive areas.
KEEP explosive dust or air-solvent mixtures AWAY from the unit.
The CO2 incubator CB does not dispose of any measures of explosion protection.
DANGER
Explosion hazard. Danger of death.
Do NOT introduce any substance into the CO2 incubator which is combustible or
explosive at working temperature.
NO explosive dust or air-solvent mixture in the inner chamber.
Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature inside the chamber must lie below the flash point or below the sublimation point of the charging material. Familiarize yourself with the physical and chemical properties of the charging material, as well as the contained moisture constituent and its behavior with the addition of heat energy.
Familiarize yourself with any potential health risks caused by the charging material, the contained moisture constituent or by reaction products which may arise during the temperature process. Take adequate measures to exclude such risks prior to putting the CO
incubator into operation.
2
DANGER
Electrical hazard. Danger of death.
The unit must NOT become wet during operation or maintenance.
The CO2 incubators were produced in accordance with VDE regulations and were routinely tested in accordance to VDE 0411-1 (IEC 61010-1).
CB (E6) 09/2013 Page 15/144
CAUTION
The glass doors and the inner chamber will become hot during operation. Danger of burning.
Do NOT touch the glass doors, the inner surfaces or the charging material during
operation.
WARNING
Stability hazard. Danger of injury. Damage to the unit and the charging material. Housing cover breakaway.
Do NOT climb on the lower housing cover. Do NOT load the lower housing cover with heavy objects while the unit door is open.

1.6 Precautions when working with gases

Notes on handling carbon dioxide (CO2)
Carbon dioxide (CO and therefore practically imperceptible. Vent out any CO or a suitable connection to an exhaust system. We recommend installing a CO
) in high concentrations is hazardous to health. It is colorless and almost odorless
2
gas that may escape via good room ventilation
2
WARNING
High concentration of CO2 (> 4 Vol.-%). Risk of death by suffocation. Danger of poisoning.
Do NOT set up units in non-ventilated recesses.
Ensure technical ventilation measures. Observe the relevant regulations for handling CO
.
2
warning system
2
CB (E6) 09/2013 Page 16/144
t and necessary safety
ition sources in areas where oxygen enrichment is
Unit with O
Oxygen (O
control: Notes on handling oxygen (O2)
2
) is colorless and almost odorless and therefore practically imperceptible. It promotes burns,
2
which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes and hair. O
is heavier than air and may accumulate in low-lying areas.
2
WARNING
High concentration of O2 (> 21 % O2). Fire and explosion hazard through contact of combustible materials with O Risk of burns and other injuries.
Do NOT set up units in non-ventilated recesses.
Ensure technical ventilation measures. Observe the relevant regulations for handling O
.
2
Take appropriate measures to prevent oxygen enrichment and fire and explosion hazards in areas where oxygen enrichment is possible.
General information for safe handling of oxygen:
Make sure training of personnel on hazards of oxygen enrichmen measures.
Make sure adequate labeling of all oxygen equipment and facilities.
Make sure gas tightness of all gas connections by checking them for leaks (e.g. with leak
spray or diluted soap solution).
.
2
Close the main valve of the source of oxygen after work when not using the chamber.
Never lubricate O
equipment with oil or fat. Use only materials and spare parts which are
2
approved for use with oxygen.
Regularly inspect fire extinguishers for proper condition.
Set up emergency showers where oxygen enrichment is possible.
Strictest smoking ban and no ign
possible.
Make sure good ventilation of areas where oxygen enrichment is possible (location of the chamber and/or O
cylinders.
2
Persons who may have been in a possibly oxygen-enriched atmosphere must keep away from ignition sources (flames, cigarettes, etc.) and ventilate their clothes at least 15 minutes.
Always keep emergency routes free.
Unit with O2 control: Notes on handling nitrogen (N2)
Nitrogen (N therefore practically imperceptible. Any N
) in high concentrations is hazardous to health. It is colorless and almost odorless and
2
gas that may escape must be safely led out via good room
2
ventilation or a suitable connection to an exhaust system.
WARNING
High concentration of N2. Risk of death by suffocation.
Do NOT set up units in non-ventilated recesses.
Ensure technical ventilation measures. Observe the relevant regulations for handling N
.
2
CB (E6) 09/2013 Page 17/144

1.7 Precautions when handling gas cylinders

General information for safe handling of gas cylinders:
Store and use gas cylinders only in well-ventilated locations.
Open the gas cylinder valve slowly to avoid pressure surges.
Secure gas cylinders during storage and use against falling (chaining).
Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them.
Always close the valve even with apparently empty cylinders; screw on the cap when not in
use. Return gas cylinders with the valve closed.
Do not open gas cylinders by force. Mark them when damaged.
Protect gas cylinders against fire, e.g. do not store together with flammable liquids.
Observe relevant regulations for dealing with gas cylinders.
Secure the gas cylinders against falling and other mechanical damage.
WARNING
Safety valve tearing off. Sudden release of the stored pressure energy. Risk of injury.
Secure gas cylinders against falling (chaining). Transport gas cylinders with a cylinder cart.
The valve of the gas cylinder always must be closed before screwing on or unscrewing the gas hose.
WARNING
Opening the cylinder valve when the cylinder is not connected. Sudden release of the stored pressure energy. Risk of injury.
Close the gas cylinder valve before connecting or removing the gas hose.
After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution).

1.8 Intended use

Series CB incubators are suitable for the cultivation of mammal cells under typical conditions of approx. 37 °C / 98.6°F. The incubator permits setting defined pH conditions by common NaHCO of commercial cell media by keeping an exact CO humidity inside to avoid osmolarity increasing caused by the evaporation of the cell media.
With the unit with O cells.
Following the instructions in this operating manual and conducting regular maintenance work (chap. 19.1) are part of the intended use.
control, a variable oxygen atmosphere can additionally influence the growth of the
2
atmosphere inside. CB incubators guarantee high
2
buffer systems
3
Other applications are not approved.
WARNING: If customer should use a BINDER chamber running in non-supervised continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples to split such specimen or samples and store them in at least two chambers, if this is feasible.
CB (E6) 09/2013 Page 18/144

2. Unit description

The CO2 incubators CB are equipped with a multifunctional microprocessor display controller for temperature, CO degree resp. 0.1 vol.-%
The inner chamber, the pre-heating chamber and the inside of the doors are all made of stainless steel (material no. 1.4301 (V2A) in Germany). The inner surfaces are smooth and therefore easy to clean. The inner chamber is deep-drawn from one piece, polished (suitable for pharmaceutical applications) and has no welds or inaccessible corners. The hinges and the seal of the inner glass door are glued from the outside to aid cleaning of the inner chamber. When operating the chamber at high temperatures (sterilization), the impact of the oxygen in the air may cause discoloration of the metallic surfaces (yellowish-brown or blue) by natural oxidation processes. These colorations are harmless and will in no way impair the function or quality of the unit.
The perforated shelves are also made of stainless steel. You can insert a maximum of 3 (CB 60), 6 (CB
160), resp. 8 (CB 220) shelves. The housing is RAL 7035 powder-coated. All corners and edges are also completely coated.
, and O2 (unit with O2 control) levels and a digital display accurate to one-tenth of a
2
The heating system of the CO
incubator permits hot-air auto-sterilization at a setpoint of 187.5 °C /
2
369.5°F. Thus, a temperature of 180 °C / 356°F is maintained for at least 30 minutes on all internal surfaces, resulting in sterilization of the entire inner chamber. Therefore, this procedure meets all international guidelines regarding hot air sterilization, e.g. AAMI ST63, DIN 58947, European Pharmacopoeia.
Thanks to the standard safety device (class 3.1 according to DIN 12880), the set temperature is maintained in case of failure.
The gas enters the chamber via a fine filter (aseptic filter) with a high filtration efficiency that also filters the smallest particles.
A highly precise, drift-free CO
gas through a special proprietary gas mixing head developed by BINDER allows precise and
CO
2
constant CO The CO
and O2 sensors
CO
2
concentrations for long periods. This creates optimum growth conditions for cultures.
2
incubator is also available with O2 control in addition to CO2 control.
2
Fast reaction times, maximum accuracy and selectivity characterize the CO the CB incubator series. The accuracy of the CO
infrared measuring system in combination with the permanent mixture of
2
measuring procedure of
measuring system is based on a double-beam
2
2
infrared measuring cell with NDIR (non-dispersive infrared) sensor, which continuously regulates to a reference value. Therefore, disturbance variables and aging phenomena in the measuring system are almost completely eliminated, so that this measuring system, in contrast to other measuring procedures, remains practically drift-free between calibrations and is entirely selective for CO
and
2
humidity. The sensor is built into the incubator and can be sterilized. The O The accuracy of the indicated values of CO
pressure (approx. 0.08 vol.-% per 10 mbar / 0.15 psi). To compensate for this effect in the CO
sensor is a semiconductor gas sensor with ZrO2 ceramic.
2
and O2 (unit with O2 control) depends on the ambient air
2
2
measurement, the controller measures the ambient air pressure and automatically includes it in the calculation.
The CO
incubators CB are equipped with an Ethernet interface for computer communication, e.g. with
2
the communication software APT-COM™ 3 DataControlSystem (option, chap. 15.1). For further options, see chap. 22.5.
Temperature range: 7 °C / 12.6 °F above ambient temperature up to +60 °C / 140 °F
range: 0 vol.-% up to 20 vol.-%)
CO
2
O
range (unit with O2 control): 0.2 vol.-% up to 95 vol.-%
2
CB (E6) 09/2013 Page 19/144

2.1 Unit overview

Instrument panel with
microprocessor controller
T4.12 and USB interface
Door handle
Unit door
Main power switch
Figure 3: CO2 incubator CB (example: model CB 160)

2.2 Instrument panel

5,7" controller display
Context-sensitive buttons
USB interface
Operating button
Pilot lamp: ready for operation
Figure 4: Instrument panel with microprocessor controller T4.12 and USB interface
CB (E6) 09/2013 Page 20/144
FRO NT
(A)
(I) ( ( (
(B) (C) (D) (E)
(G)
(H)

2.3 Inner chamber

J) K) L)
Figure 5: CB 160 with O
control and options
2
CB (E6) 09/2013 Page 21/144
(B)
(C)
(D)
(D2) (F)
(G)
(E)
Figure 6: CB 160 / CB 220 with O
control and options
2
(A) Instrument panel with microprocessor controller T4.12, indicating temperature and CO
(unit with O2 control)”
O
2
(B) Connection socket for extra-low voltage supply (option, chap. 15.6) (C) CO (D) Gas mixing head CO (D2) Additional gas mixing head O
and humidity sensor
2
2
/ N2 (unit with O2 control)
2
(E) Pt 100 temperature probe (F) O
sensor (unit with O2 control)
2
(G) Internal socket 230V (max. 3 A) (option, chap. 15.4) (H) Perforated shelves, made of stainless steel (I) Measuring access port (J) Inner glass doors (K) Permadry™ water pans (L) Lower housing cover
as well as
2
CB (E6) 09/2013 Page 22/144

2.4 Control panel on the rear of the unit

(1) (2) (3) (6) (4) (5)
(7) (8) (9) (10)
Figure 7: Rear control panel CB with O
control and options
2
(1) Miniature fuse (2) External socket for extra-low voltage supply (option for CB 160 / CB 220, chap. 15.6) (3) DIN socket for analog outputs 4-20 mA (option, chap. 15.5) (4) Quick acting closure socket for CO (5) Quick acting closure socket for O (6) Quick acting closure socket for N
2
(unit with O2 control)
2
(unit with O2 control)
2
(7) Socket for IEC connector plug for power cable (8) Ethernet interface for computer communication (9) DIN-socket for zero-voltage relay alarm outputs (10) RS 422 interface for computer communication (option)
CB (E6) 09/2013 Page 23/144

3. Completeness of delivery, transportation, storage, and installation

3.1 Unpacking, and checking equipment and completeness of delivery

After unpacking, please check the unit and its optional accessories, if any, based on the delivery receipt for completeness and for transportation damage. Inform the carrier immediately if transportation damage has occurred.
The final tests of the manufacturer may have caused traces of the shelves on the inner surfaces. This has no impact on the function and performance of the unit.
Please remove any transportation protection devices and adhesives in/on the unit and on the doors and remove the operating manuals and accessory equipment.
Remove any protective lamination sheet on the inner metal surfaces prior to commissioning.
CAUTION
Sliding or tilting of the unit. Damage to the unit. Risk of injury by lifting heavy loads.
Do NOT lift or transport the unit using the door handle, the door or the lower housing. Lift the unit from the pallet at the four lower corners with the aid of four people.
If you need to return the unit, please use the original packing and observe the guidelines for safe lifting and transportation (chap. 3.2).
For disposal of the transport packing, see chap. 20.1.
Note on second-hand units (Ex-Demo-Units):
Second-hand units are units that were used for a short time for tests or exhibitions. They are thoroughly tested before resale. BINDER ensures that the chamber is technically sound and will work flawlessly.
Second-hand units are marked with a sticker on the unit door. Please remove the sticker before commissioning the unit.

3.2 Guidelines for safe lifting and transportation

After operation, please observe the guidelines for temporary decommissioning (chap. 20.2). Empty the Permadry™ water pan before moving the incubator. In case of any spilling of the contents, shut down the incubator and dry it out carefully and completely.
CB (E6) 09/2013 Page 24/144
CAUTION
Sliding or tilting of the unit. Damage to the unit. Risk of injury by lifting heavy loads.
Transport the unit in its original packaging only. For moving or shipping, secure the unit with transport straps.
Do NOT lift or transport the unit using the door handle, the door or the lower housing.
Lift the unit at the four lower corners with the aid of 4 people and place it on a rolling
pallet.
Move the unit to the desired location and lift it from the rolling pallet with the aid of four
people.
Permissible ambient temperature range during transport: 10 °C / 14 °F to +60 °C / 140 °F.
You can order transport packing for moving or shipping purposes from BINDER service.

3.3 Storage

Intermediate storage of the unit is possible in a closed and dry room. Observe the guidelines for temporary decommissioning (chap. 20.2).
Permissible ambient temperature range during storage: -10 °C / 14 °F to +60 °C / 140 °F.
Permissible ambient humidity: max. 70 % r.H., non-condensing
When after storage in a cold location you transfer the unit to its warmer installation site, condensation may form. Before start-up, wait at least one hour until the chamber has attained ambient temperature and is completely dry.

3.4 Location of installation and ambient conditions

Notes on the location of installation
Set up the CO The chambers are designed for setting up inside a building (indoor use).
Freestanding CB incubators are suitable for installation on tables or on the optionally available stand (height 200 mm / 0.5 ft). Note: The site of installation must be capable of supporting the unit’s weight (see technical data, chap.22.4).
Align the unit using a spirit level to ensure even covering of the cell-cultures with the medium. For this purpose, manually adjust the four incubator feet.
incubators can be stacked on top of each other (two units maximum). For safe stacking that is easy
CO
2
to maintain, use the original BINDER stacking stand (chap. 15.8.1) or the stacking adapter (chap. 15.8.2).
incubator on a flat, even surface, free from vibration and in a well-ventilated, dry location.
2
CAUTION
Sliding of stacked unit. Damage to the units.
When stacking, use rubber pads (Art. No. 8012-0376) for the feet of the upper
incubator.
To completely separate the unit from the power supply, you must disconnect the power plug. Install the unit in a way that the power plug is easily accessible and can be easily pulled in case of danger.
In order to avoid contamination, never place the unit directly on the floor.
CB (E6) 09/2013 Page 25/144
CAUTION
Danger of overheating. Damage to the unit.
Do NOT set up units in non-ventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
Do not install or operate the CO2 incubator CB in potentially explosive areas.
DANGER
Explosion hazard. Danger of death.
Do NOT operate the unit in potentially explosive areas.
KEEP explosive dust or air-solvent mixtures AWAY from the vicinity of the unit.
Ambient conditions
Permissible ambient temperature range for operation: +18 °C / 64.4 °F to +30 °C / 86 °F
At elevated ambient temperature values, fluctuations in temperature can occur.
Ideal ambient temperature: at least 7 °C / 12.6 °F below the intended working temperature. E.g.,
working temperature 37 °C / 98.6 °F = ambient temperature 30 °C / 86 °F and lower. In the event of working temperatures of less than 7 °C / 12.6 °F above the ambient temperature, the setpoint can be exceeded.
The ambient temperature should not be substantially higher than the indicated ambient temperature of 22 ±3 °C / 71.6 ±5.4 °F to which the specified technical data relates. For other ambient conditions, deviations from the indicated data are possible.
Avoid direct solar radiation on the unit.
Permissible ambient humidity: 70 % r.H. max., non-condensing.
Installation height: max. 2000 m / 6561.7 ft above sea level.
Wall distances: rear 100 mm / 3.94 in, sides 50 mm / 1.97 in.
CB (E6) 09/2013 Page 26/144
Notes on handling carbon dioxide (CO
Carbon dioxide (CO
) in high concentrations is hazardous to health. It is colorless and almost odorless
2
and therefore practically imperceptible. Vent out any CO or a suitable connection to an exhaust system. We recommend installing a CO
)
2
gas that may escape via good room ventilation
2
warning system.
2
WARNING
High concentration of CO2 (> 4 Vol.-%). Danger of death by suffocation. Danger of poisoning.
Do NOT set up units in non-ventilated recesses.
Ensure technical ventilation measures. Observe the relevant regulations for handling CO
Observe the occupational exposure limit OEL for CO2 set by the national authorities (formerly maximum permitted workplace concentration). Check compliance when operating all units located in the room.
3
OEL for Germany: 5000 ml/m
CO
lost with each opening the door: about 16.4 g, i.e. 0.0084 cubic meters / 0.296 cubic feet (under
2
(ppm) = 0,5 Vol.-%
normal pressure)
CO
lost during 12h at 5 vol.-% without opening the door: approx. < 2 g, i.e. 0.001 cubic meter / 0.035
2
cubic feet (under normal pressure 1013 mbar / 14.7 psi)
.
2
An example of how to evaluate laboratory volume and air change rate:
Question: Is an air change rate of 1/h sufficient for a lab with a volume of 100 cubic meters /
3,531.5 cubic feet with 10 incubators CB, opened 4 times per hour?
Calculation: CO
concentration = CO2 lost by opening the door, multiplied by 10 units, multiplied
2
by opening the door 4 times per hour, divided by lab volume
0.0084 cubic meters x 10 x 4 div. 100 cubic meters = 0.00336, i.e. 0.336 % or 3360 ppm.
0.296 cubic feet x 10 x 4 div. 3,531.5 cubic feet = 0.00336, i.e. 0.336 % or 3360 ppm. Result: The maximum permissible value of 5000 ppm is not exceeded under these operation
conditions.
Unit with O
Oxygen (O
control: Notes on handling oxygen (O2)
2
) is colorless and almost odorless and therefore practically imperceptible. It promotes burns,
2
which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes and hair. O
is heavier than air and may accumulate in low-lying areas.
2
WARNING
High concentration of O2 (> 21 % O2). Fire and explosion hazard through contact of combustible materials with O Risk of burns and other injuries.
Do NOT set up units in non-ventilated recesses.
Ensure technical ventilation measures. Observe the relevant regulations for handling O
.
2
.
2
Take appropriate measures to prevent oxygen enrichment and fire and explosion hazards in areas where oxygen enrichment is possible.
CB (E6) 09/2013 Page 27/144
t and necessary safety
Strictest smoking ban and no ignition sources in areas where oxygen enrichment is
from ignition sources (flames, cigarettes, etc.) and ventilate their clothes at least 15
General information for safe handling of oxygen:
Make sure training of personnel on hazards of oxygen enrichmen measures.
Make sure adequate labeling of all oxygen equipment and facilities.
Make sure gas tightness of all gas connections by checking them for leaks (e.g. with leak
spray or diluted soap solution).
Close the main valve of the source of oxygen after work when not using the chamber.
Never lubricate O
equipment with oil or fat. Use only materials and spare parts which are
2
approved for use with oxygen.
Regularly inspect fire extinguishers for proper condition.
Set up emergency showers where oxygen enrichment is possible.
possible.
Make sure good ventilation of areas where oxygen enrichment is possible (location of the chamber and/or O
cylinders.
2
Persons who may have been in a possibly oxygen-enriched atmosphere must keep away
minutes.
Always keep emergency routes free.
Unit with O
Nitrogen (N therefore practically imperceptible. Any N
control: Notes on handling nitrogen (N2)
2
) in high concentrations is hazardous to health. It is colorless and almost odorless and
2
gas that may escape must be safely led out via good room
2
ventilation or a suitable connection to an exhaust system.
WARNING
High concentration of N2. Risk of death by suffocation.
Do NOT set up units in non-ventilated recesses.
Ensure technical ventilation measures. Observe the relevant regulations for handling N
.
2
CB (E6) 09/2013 Page 28/144
Figure 8: Permadry™ water pan CB 160 / CB 220
Figure 9: Permadry™ water pan CB 60

4. Installation and connections

4.1 Shelves

You can put the shelves in different positions at the line of channel beads in the inner chamber. Hold the shelf straight and then insert it so it will go smoothly inside the unit.
Permitted shelf loads:
Maximum load of one single shelf: 10 kg / 22 lb Maximum total load of all shelves: 30 kg / 66 lb

4.2 Permadry™ water pan

The Permadry™ system developed by BINDER is an effective and easy to handle system that ensures high humidity inside the incubator without any condensation forming on the inner surfaces. The Permadry™ water pan consists of two pans in which the outer one is heated and the inner one cooled. With the slight difference of temperature caused by that cooling, the central pan is the specific point for condensation of the surplus humidity. Therefore, all other inner surfaces remain dry.
Put the Permadry™ water pan on the bottom of the inner chamber in a way that both notches lock into
place.
The front side of the Permadry™ water pan is marked “FRONT”.
Figure 10: Letters “FRONT” indicating the front of the Permadry™ water pan
Please make sure that the Permadry™ water pan has firm contact to the inner chamber bottom and
rests tightly on it.
Fill only the outer pan with distilled, sterilized water up to the filling level marking on the edge of the
inner pan. Maximum filling quantity of the outer pan: CB 60: approx. 0.7 liters, CB 160 and CB220): approx.
2 liters.
Figure 11: Filling height line of the outer basin CB 160 / CB 220
CB (E6) 09/2013 Page 29/144
We recommend cleaning and refilling the pans 2 to 3 times a week. For evacuation, remove the
Permadry™ water pan.
We recommend using distilled, sterile water to achieve optimum growth results. Any corrosive damage
that may arise following the use of water of different quality or by additives is excluded from the liability agreement.
If required, you can add microbiologically inhibiting substances such as copper chips, copper sulfate
or ethylene diamine tetra-vinegar acid (EDTA) in a concentration of 1 to 5 mmol/l.
Empty the Permadry™ water pan before moving the incubator. In case of the contents spilling, immediately shut down the incubator and dry it carefully and completely.

4.3 Gas connections

General information for safe handling of gas cylinders:
Store and use gas cylinders only in well ventilated areas.
Open the gas cylinder valve slowly to avoid pressure surges.
Secure gas cylinders during storage and use against falling (chaining).
Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them.
Always close the valve even with apparently empty cylinders; screw on the cap when not in
use. Return gas cylinders with the valve closed.
Do not open gas cylinders by force. Mark them when damaged.
Protect gas cylinders against fire, e.g. do not store together with flammable liquids.
Observe relevant regulations for dealing with gas cylinders.
Secure the gas cylinder against falling and other mechanical damage.
WARNING
Safety valve tearing off. Sudden release of the stored pressure energy. Risk of injury.
Secure gas cylinders against falling (chaining). Transport gas cylinders with a cylinder cart.
The valve of the gas cylinder always must be closed before screwing on or unscrewing the gas hose.
WARNING
Opening the cylinder valve when the cylinder is not connected. Sudden release of the stored pressure energy. Risk of injury.
Close the gas cylinder valve before connecting or removing the gas hose.
After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution).
CB (E6) 09/2013 Page 30/144

4.3.1 Connection of the CO2 gas cylinder

Carbon dioxide (CO2) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Vent out any CO or a suitable connection to an exhaust system. We recommend installing a CO
High concentration of CO2 (> 4 Vol.-%). Danger of death by suffocation.
Do NOT set up units in non-ventilated recesses.
Ensure technical ventilation measures. Observe the relevant regulations for handling CO
The CO2 gas necessary for operation must have a technical grade of 99.5 %.
The gas connections must be established by qualified personnel who are trained in handling the respective gases and familiar with the required safety measures.
gas that may escape via good room ventilation
2
WARNING
2
.
warning system.
2
The following steps are required:
Ensuring the correct CO
output pressure
2
A gas supply pressure above 2.5 bar / 36 psi will result in unit damage.
Use a pressure reducer and make sure to avoid any excessive outlet pressure when connecting the gas hose to the incubator.
The real outlet pressure of gas cylinders, sets of gas cylinders or central gas supplies am on the second manometer must not exceed 2.5 bar / 36 psi.
CAUTION
Excessive outlet pressure > 2.5 bar / 36 psi. Damage to the unit.
The outlet pressure must NOT exceed the indicated value of 2.5 bar / 36 psi.
Before connecting, check the outlet pressure on the pressure reducer of the
cylinder.
Adjust the outlet pressure to 2.0 bar / 29 psi above the ambient pressure.
Observe the correct outlet pressure also when replacing the gas cylinders.
Establishing the connection to the incubator
Connect the supplied gas hose (internal diameter 6 mm / 0.24 inches) to the pressure reducer of the gas cylinders or central gas supply and secure the connection with the supplied hose clamp.
Connect the pre-assembled hose nozzle of the gas hose to the quick acting closure socket (4) DN 6 on the unit rear, as described in chap. 4.3.4.
Leak test
After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution).
CB (E6) 09/2013 Page 31/144
The recovery times of the gas concentrations inside the chamber after opening the door are indicated in the technical data (chap. 22.4) and refer to a connection pressure of 2.0 bar / 29 psi. Decreasing supply pressure will result in longer recovery times.
Conversion table for gas inlet pressures, bar – psi, see chap. 22.8.

4.3.2 Connection of the O2 gas cylinder (unit with O2 control)

Note: Do not connect the O2 cylinder or disconnect the gas supply (by pulling off the gas hose) when operating at setpoints below 19 vol.-% O
.
2
Oxygen (O2) is colorless and almost odorless and therefore practically imperceptible. It promotes burns, which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes and hair. O
is heavier than air and may accumulate in low-lying areas.
2
WARNUNG
High concentration of O2 (> 21 % O2). Fire and explosion hazard through contact of combustible materials with O Risk of burns and other injuries.
Do NOT set up units in non-ventilated recesses.
Ensure technical ventilation measures. Observe the relevant regulations for handling O
.
2
The O2 gas necessary for operation must have a technical grade of 99.5 %.
.
2
The gas connections must be established by qualified personnel who are trained in handling the respective gases and familiar with the required safety measures.
The following steps are required:
Ensuring the correct O
output pressure
2
A gas supply pressure above 2.5 bar / 36 psi will result in unit damage.
Use a pressure reducer and make sure to avoid any excessive outlet pressure when connecting the gas hose to the incubator.
The real outlet pressure of gas cylinders, sets of gas cylinders or central gas supplies am on the second manometer must not exceed 2.5 bar / 36 psi.
CAUTION
Excessive outlet pressure > 2.5 bar / 36 psi. Damage to the unit.
The outlet pressure must NOT exceed the indicated value of 2.5 bar / 36 psi.
Before connecting, check the outlet pressure on the pressure reducer of the
cylinder.
Adjust the outlet pressure to 2.0 bar / 29 psi above the ambient pressure.
Observe the correct outlet pressure also when replacing the gas cylinders.
CB (E6) 09/2013 Page 32/144
Establishing the connection to the incubator
Connect the supplied gas hose (internal diameter 6 mm / 0.24 inches) to the pressure reducer of the gas cylinders or central gas supply and secure the connection with the supplied hose clamp.
Connect the pre-assembled hose nozzle of the gas hose to the quick acting closure socket (5) DN 6 on the unit rear, as described in chap. 4.3.4.
Leak test
After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution).
The recovery times of the gas concentrations inside the chamber after opening the door are indicated in the technical data (chap. 22.4) and refer to a connection pressure of 2.0 bar / 29 psi. Decreasing supply pressure will result in longer recovery times.
Conversion table for gas inlet pressures, bar – psi, see chap. 22.8.

4.3.3 Connection of the N2 gas cylinder (unit with O2 control)

Nitrogen (N2) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Any N ventilation or a suitable connection to an exhaust system.
gas that may escape must be safely led out via good room
2
WARNING
High concentration of N2. Risk of death by suffocation.
Do NOT set up units in non-ventilated recesses.
Ensure technical ventilation measures. Observe the relevant regulations for handling N
The N2 gas necessary for operation must have a technical grade of 99.5 %.
The gas connections must be established by qualified personnel who are trained in handling the respective gases and familiar with the required safety measures.
The following steps are required:
Ensuring the correct N
output pressure
2
A gas supply pressure above 2.5 bar / 36 psi will result in unit damage.
Use a pressure reducer and make sure to avoid any excessive outlet pressure when connecting the gas hose to the incubator.
The real outlet pressure of gas cylinders, sets of gas cylinders or central gas supplies am on the second manometer must not exceed 2.5 bar / 36 psi.
.
2
CB (E6) 09/2013 Page 33/144
CAUTION
Excessive outlet pressure > 2.5 bar / 36 psi. Damage to the unit.
The outlet pressure must NOT exceed the indicated value of 2.5 bar / 36 psi.
Before connecting, check the outlet pressure on the pressure reducer of the
cylinder.
Adjust the outlet pressure to 2.0 bar / 29 psi above the ambient pressure.
Observe the correct outlet pressure also when replacing the gas cylinders.
Establishing the connection to the incubator
Connect the supplied gas hose (internal diameter 6 mm / 0.24 inches) to the pressure reducer of the gas cylinders or central gas supply and secure the connection with the supplied hose clamp.
Connect the pre-assembled hose nozzle of the gas hose to the quick acting closure socket (6) DN 6 on the unit rear, as described in chap. 4.3.4.
Leak test
After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution).
The recovery times of the gas concentrations inside the chamber after opening the door are indicated in the technical data (chap. 22.4) and refer to a connection pressure of 2.0 bar / 29 psi. Decreasing supply pressure will result in longer recovery times.
Conversion table for gas inlet pressures, bar – psi, see chap. 22.8.

4.3.4 Connecting the gas hose to the unit rear (for CO2, O2, N2)

The procedure of connecting the gas hose to the unit rear is the same for any gas connection. All quick acting closure sockets (CO
, and N2 and O2 on unit with O2 control) are degreased and supplied with a
2
FKM gasket.
Note for units with O
connection (unit with O
control: The quick acting closure socket and the hose nozzle for the O2
2
control) must be degreased.
2
WARNING
Fire and explosion hazard through contact of fat with O2. Risk of burns and other injuries.
All connection parts for the O
The gas hose, which will be used to establish the connection to a gas cylinder, is already attached to the hose nozzle and secured by a hose clamp. Plug the hose nozzle into the corresponding quick acting closure socket (a) located at the rear of the unit. This quick acting closure socket is closed by a rubber cover (b).
connection must be degreased.
2
Only use the supplied hose nozzle to connect to the quick acting closure socket. Otherwise, the quick acting closure socket may leak, and/or it may become impossible to
connect the original hose nozzle. In this case, please contact BINDER Service.
CB (E6) 09/2013 Page 34/144
Gas cylinder connection kits are available for CO2 (Art. No. 8012-0014), O2
(a)
(c)
(b)
Remove the rubber cover (b) by pulling it off.
Unit rear
Figure 12: Connecting the hose lead to the gas cylinder
Now fit the hose nozzle (c) in the quick acting closure socket. To remove the connection, pull the hose nozzle off the quick acting closure socket.

4.3.5 Gas cylinder connection kits (option)

(Art. No. 8012-0015) and N2 (Art. No. 8012-0016). The connection kit includes the following parts for connecting a gas cylinder
to the CO
Pressure reducer with manometers for cylinder pressure (high pressure gauge) and outlet pressure (low pressure gauge)
5 m pressure hose with pre-assembled hose nozzle for quick acting closure socket
1 hose clamp to connect the gas hose to the pressure reducer
Figure 13: Gas cylinder connection kit
Instructions 7001-0249 included with the connection kit describe connecting and setting the gas cylinder connection kit.
incubator:
2
Excessive outlet pressure > 2.5 bar / 36 psi. Damage to the unit.
The outlet pressure must NOT exceed the indicated value of 2.5 bar / 36 psi.
Before connecting, check the outlet pressure on the pressure reducer of the cylinder. Adjust the outlet pressure to 2.0 bar / 29 psi above the ambient pressure.
The gas connections must be established by qualified personnel who are trained in handling the respective gases and familiar with the required safety measures.
CB (E6) 09/2013 Page 35/144
CAUTION

4.4 Electrical connection

The CO2 incubator comes with an IEC connector plug.
Model
Art. No. (x = 0 or 1)
Power plug
Voltage +/-10 %
Power frequency
Unit fuse
CB 60 9x40-0088 Shock-proof plug 230 V (1N~) 50/60 Hz 16 A
CB 60-UL 9x40-0089 NEMA 5-15P 100-120 V (1N~) 60 Hz 16 A
CB 60 with O
control
2
CB 60-UL with O
control
2
9x40-0090 Shock-proof plug 230 V (1N~) 50/60 Hz 16 A
9x40-0091 NEMA 5-15P 100-120 V (1N~) 60 Hz 16 A
CB 160 9x40-0092 Shock-proof plug 230 V (1N~) 50/60 Hz 16 A
CB 160-UL 9x40-0093 NEMA 5-20P 100-120 V (1N~) 60 Hz 16 A
CB 160 with O
control
2
CB 160-UL with O
control
2
9x40-0094 Shock-proof plug 230 V (1N~) 50/60 Hz 16 A
9x40-0095 NEMA 5-20P 100-120 V (1N~) 60 Hz 16 A
CB 220 9x40-0096 Shock-proof plug 230 V (1N~) 50/60 Hz 16 A
CB 220-UL 9x40-0097 NEMA 5-20P 100-120 V (1N~) 60 Hz 16 A
CB 220 with O
control
2
CB 220-UL with O
control
2
9x40-0098 Shock-proof plug 230 V (1N~) 50/60 Hz 16 A
9x40-0099 NEMA 5-20P 100-120 V (1N~) 60 Hz 16 A
Prior to connection and start-up, check the power supply voltage. Compare the values to the specified data located on the unit’s type plate (left unit side, bottom right-hand, see chap. 1.4).
When connecting, please observe the regulations specified by the local electricity supply company as well as the VDE directives (for Germany).
Pollution degree (acc. to IEC 61010-1): 2
Over-voltage category (acc. to IEC 61010-1): II
CAUTION
Danger of incorrect power supply voltage. Damage to the equipment.
Check the power supply voltage before connection and start-up. Compare the power supply voltage with the data indicated on the type plate.
See also electrical data (chap. 22.4).
To completely separate the unit from the power supply, you must disconnect the power plug. Install the unit in a way that the power plug is easily accessible and can be easily pulled in case of danger.
CB (E6) 09/2013 Page 36/144
with elongated holes to
4.5 Handling and aligning the multiple-divided inner glass door, gas proof
(optional equipment)
Rubber profile gasket of individual glass door of the divided inner glass door
Hinge of the set-in divided inner glass door,
align the glass door
Measuring access port
Figure 14: Multiple-divided inner glass door, gas proof, for CB 160
Pull the handles to open each of the individual glass doors. Do not press too hard while closing them
to avoid pushing the doors through the gasket into the inner chamber.
The rubber profile gaskets of the individual glass doors are easy to replace.
If the divided inner glass door is not in an aligned position, fasten the hinge screws in the elongated
holes of the hinge to align it. Align the door and operate the locking smoothly.
CB (E6) 09/2013 Page 37/144

5. Start up

After connecting the supply lines (chap. 4.4) turn on the chamber by the main power switch. The pilot lamp shows the unit is ready for operation.
Observe a delay time of about 30s between turning Off and On again. Otherwise an initialization problem may occur.
Note that the chamber is in stand-by mode when the main power switch has been turned on and yet the controller display is dark. Turn on the unit by pressing any controller button.
Warming chambers may release odors in the first few days after commissioning. This is not a quality defect. To reduce odors quickly we recommend heating up the chamber to its nominal temperature for one day and in a well-ventilated location.
WARNING: If customer should use a BINDER chamber running in non-supervised continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples to split such specimen or samples and store them in at least two chambers, if this is feasible.

5.1 Equilibration time

Temperature: Equilibration time is approx. 1 hour.
: After approx. 5 minutes, the CO2 concentration equilibrates automatically to the pre-set value of 5
CO
2
vol.-% CO
(unit with O2 control): After a delay of 10 minutes, the ambient oxygen concentration of approx. 20.7
O
2
vol.-% is displayed. During the first 10 minutes, O
.
2
control is not defined. Then O2 is regulated to the
2
displayed setpoint value of 20.7 vol.-%.

5.2 Factory settings

The unit is supplied with the following basic preset parameters:
Temperature setpoint 37 °C / 98.6°F
CO
O
Safety controller class 3.1 38.5 °C / 101.3°F
Sterilization temperature 187.5 °C / 369.5°F
Audible alarm signal (buzzer) turned on
concentration 5 vol.-%
2
concentration (unit with O2 control) 20.7 vol.-%
2
The set temperature determines the target working temperature in the inner chamber, i.e. set value 37 °C / 98.6°F = target working temperature 37 °C / 98.6°F. The same is valid for the CO (unit with O
control). For the hot-air sterilization, the set value is 187.5 °C / 369.5°F and cannot be
2
and O2 concentration
2
changed.
As long as there is a difference between the actual and set value shown in the display, adequate operation of the unit is not guaranteed.
CB (E6) 09/2013 Page 38/144

6. Functional overview of the T4.12 chamber controller

The T4.12 chamber controller controls the following values inside the CO2 incubator:
Temperature in °C (range by 7 °C / 12.6 °F above ambient temperature up to +60 °C / 140 °F)
Carbon dioxide concentration in vol.-% (range 0 vol.-% up to 20 vol.-%)
Unit with O
Oxygen concentration in vol.-% (range 0.2 vol.-% up to 95 vol.-%)
control in addition:
2
You can enter the desired setpoint values in fixed value operating mode in the display controller. The controller also offers various notifications and alarm messages with visual and audible indication, a trace file and remote alarms via e-mail. Programming can be done directly through the keypad of the controller or graphically through the software APT-COM™ 3 DataControlSystem (option, chap. 15.1) specially developed by BINDER.
Temperature values
values
CO
2
Humidity value
values
O
2
Actual values Setpoint values
Icons: Controller operating in “fixed value” operating mode
Figure 15: T4.12 microprocessor controller, initial view in “Fixed value” controller mode
(sample values, unit with O
control)
2
CB (E6) 09/2013 Page 39/144
Fixed value
Setpoint
Actual value
37.0
37.2
5.0
5.0
85.5
20.7
20.7
Menu
Fixed value
..\ Menu
Controller mode
Event list
Alarms
Setpoints
Safety controller
Import/Export
Hot-air sterilization
Humidity control

6.1 Menu structure

08.03.2013 05:05:06
Temperature [°C]
CO2 [Vol.-%]
Humidity [%rH]
O2 [Vol.-%]
User Quick menu
From the Initial view you have access to different menus using the menu buttons “User”, “Quick menu”, or “Menu”. From there you can access the desired control functions. To do this, select the function by turning the operating button and press the operating button to confirm the selection.
In any menu, you can return to the previous display pressing the "Close" button or to the initial view with the "Home" button.
Depending on the logged-in user or administrator, the available menu functions may vary. These instructions present the functions which are available to the logged-in administrator.
Initial view (sample values, unit with O2 control). Press the desired menu button.

6.1.1 General menu

The general menu provides access to all setting functions of the controller, a graphical display of the measured values, and the possibility to read and give out data via the USB interface. In addition, supporting functions like a settings wizard or a contact page are available.
08.03.2013 05:05:06
General menu
Close Home
Turn the operating button to see additional menu items.
CB (E6) 09/2013 Page 40/144
Fixed value
..\ Menu
Settings
Measurement chart
Interior socket (optional)
Sensor adjustment
Service contact
System information
Fixed value
..\ Quick menu
Measurement chart
Active alarms
Temperature setpoint
CO2 setpoint
O2 setpoint
Safety controller setpoint
Hot-air sterilization
08.03.2013 05:05:06
General menu (next page)
Close Home
Controller mode
Event list
Alarms
Setpoints
Safety controller
Import/Export
Hot-air sterilization
Humidity control
Settings
Measurement chart
Interior socket (optional)
Sensor adjustment
Service contact
System information
Switching between the operating modes “control offor “fixed value”, chap.
6.2.1
Display of status information and errors, chap. 12
Alarm settings, chap. 11.5
Setpoint entry, chap. 7.4
Setting the safety controller, chap. 14.2
Data transfer via USB interface, chap. 10
Performing a hot-air sterilization at 180 °C / 356 °F, chap. 18.3
Setting the humidity control of the Permadry™ system, chap.6.4
General controller settings, chap. 9
Graphical display of the measured values, chap. 13
Turning on / off the interior socket voltage (option), chap. 6.5
Adjustment menu for single-point and two-point adjustments (for Service)
Service information
Chamber information (model, name, serial no., firmware etc.)

6.1.2 Quick menu

The Quick menu provides fast access to frequently used functions.
Close Home
CB (E6) 09/2013 Page 41/144
08.03.2013 05:05:06
“Quick menu”. (item “O
unit with O
setpoint” only coming with
2
control)
2
Fixed value
..\ User
Key lock
Show event list
Measurement chart Graphical display of the measured values, chap. 13
Active alarms Alarm settings, chap. 11.5
Temperature setpoint Temperature setpoint entry, chap. 7.3
CO2 setpoint CO2 setpoint entry, chap. 7.3
O2 setpoint O2 setpoint, chap. 7.3
Safety controller setpoint Setting the safety controller setpoint, chap. 14.2.2
Hot-air sterilization
Performing a hot-air sterilization at 180 °C / 356 °F, chap. 18.3

6.1.3 User menu

The user menu includes the key lock function and provides quick access to the event list. The key lock function serves to block the access to the controller. An overview of logon, logoff, and other
events is given in the event list.
08.03.2013 05:05:06
“User” menu
Close Home
Key lock Configuring the key lock function, chap. 8
Show event list Displaying the event list, chap. 12
CB (E6) 09/2013 Page 42/144
Fixed value
..\ Menu
Controller mode
Event list
Alarms
Setpoints
Safety controller
Import/Export
Hot-air sterilization
Humidity control
Fixed value
..\ Controller mode
Control off
Fixed value
O2 control

6.2 Operating modes

In the “control off” mode (chap. 6.2.1), the controller is non-functional and displays only the actual values. There is no heating or refrigeration and no inlet of CO temperature approximates the ambient value.
You can enter the desired setpoint values in “fixed value” mode (chap. 7). The controller then operates as a fixed-point control, i.e., it reaches and maintains the defined setpoints until the next manual change.

6.2.1 Activating the “control offmode or change to “fixed value” operating mode

To select the “control offor “fixed value” operating mode, go to Menu > Controller mode
08.03.2013 05:05:06
General menu. Select “Controller Mode”
and press the operating button.
or O2 (unit with O2 control). The
2
Close Home
08.03.2013 05:05:06
Close Home
Submenu “Controller Mode”. Select the desired controller mode
“Control off” or “Fixed value” and press the operating button.
CB (E6) 09/2013 Page 43/144
Control off
..\ Menu
Controller mode
Event list
Alarms
Setpoints
Safety controller
Import/Export
Hot-air sterilization
Humidity control
Control off
Setpoint
Actual value
22.2
0.3
85.5
08.03.2013 05:05:06
General menu. The controller mode “Fixed value” or “Control off” is
indicated in the display headline.
Close Home
Go back to the initial view with “Home”.
08.03.2013 05:05:06
Temperature [°C]
CO2 [Vol.-%]
Humidity [%rH]
User Quick menu
The controller is non-functional, i.e., there is no heating or CO2 entry or O2 entry (unit with O2 control).
Menu
Initial view in “Control off” mode (sample values)
CB (E6) 09/2013 Page 44/144
Hyperoxic range
(>22 vol.-% O2)
Hypoxic range
(<20 vol.-% O2)
Hyperoxic and
hypoxic ranges

6.3 Deactivating the O2 control and O2/N2 pressure alarms (unit with O2 control)

6.3.1 Required gas supply of the unit with O2 control

In the unit with O2 control, cell growth can be additionally influenced by a variable oxygen content. Depending on the required range of O2 concentration, the type of gas which is required may vary:
Hyperoxic range (> 22 vol.-% O
As compared to the ambient air (O increased, no nitrogen is required for the O cylinder. You can deactivate the N
)
2
concentration of 20.9 vol.-%) only the O2 concentration needs to be
2
pressure alarm in the menu “Menu > Controller mode > O2 control
2
control. Therefore, it is not necessary to connect a N2
2
(chap. 6.3.2).
Hypoxic range (< 20 Vol.-% O As compared to the ambient air (O
be increased, no oxygen is required for the O cylinder. You can deactivate the O
)
2
concentration of 20.9 vol.-%) only the N2 concentration needs to
2
pressure alarm in the menu “Menu > Controller mode > O2
2
control. Therefore, it is not necessary to connect an O2
2
control” (chap. 6.3.2).
Hyperoxic and hypoxic range or range from 20 vol.-% O N
and O2 are required for O2 control. It is necessary to connect all gas supplies.
2
Operation without O You can deactivate the O
With this setting, no alarm messages for O
control
2
control in the menu “Menu > Controller mode > O2 control” (chap. 6.3.2).
2
and N2 (pressure or concentration) will be issued.
2
up to 22 vol.-% O2
2
Since neither nitrogen nor oxygen are required, it is not necessary to connect an O
and N2 pressure alarms will be issued with deactivated O2 control.
O
2
The zero-voltage relay alarm contact (chap. 11.6) for messages of deviation of the O will also not be triggered.
or N2 cylinder. No
2
concentration
2
Overview
No O2 control
off O2 control (with O2 concentration alarm)
active active active
Connection of O2 cylinder connected not connected connected not connected
off
O2 pressure alarm active
active off
Connection of N2 cylinder not connected connected connected not connected
off
N2 pressure alarm
active active off
CB (E6) 09/2013 Page 45/144
Fixed value
..\ O2 control
O2 control: On
O2 pressure alarm: On
N2 pressure alarm: On
Fixed value
Setpoint
Actual value
37.0
37.2
5.0
5.0
85.5
20.7
Menu
Fixed value
Setpoint
Actual value
37.0
37.2
5.0
5.0
85.5
25.0
25.0
Menu

6.3.2 Activating / deactivating the O2 control and O2 / N2 pressure alarms

To activate or deactivate the O2 control and O2 / N2 pressure alarms, go to
Menu > Controller mode > O2 control
08.03.2013 05:05:06
Close Home
Go back to the initial view with “Home”.
08.03.2013 05:05:06
Temperature [°C]
CO2 [Vol.-%]
Humidity [%rH]
O2 [Vol.-%]
Submenu “O2 control”. The current settings are displayed. To change the settings, press the operating button. The modified settings are displayed
“O2 control: On” = O “O2 control: Off” = O “O2 pressure alarm: On” or “N2 pressure alarm: On”
= the corresponding pressure alarm is activated. “O2 pressure alarm: Off” or “N2 pressure alarm: Off”
= the corresponding pressure alarm is deactivated.
Initial view with deactivated O2 control (sample values).
Also the pressure alarms for O deactivated.
control is active.
2
control is deactivated.
2
and N2 are
2
User Quick menu
To enter the O2 setpoint, first activate the O2 control again.
With active O
Temperature [°C]
CO2 [Vol.-%]
Humidity [%rH]
O2 [Vol.-%]
User Quick menu
control, the pressure alarms for O2 and N2 can be deactivated individually.
2
08.03.2013 05:05:06
Initial view with activated O2 control and deactivated
pressure alarm (sample values).
O
2
CB (E6) 09/2013 Page 46/144
Fixed value
..\ Humidity control Low/Medium/High
Humidity control Medium
Fixed value
..\ Humidity control
Low
Medium
High

6.4 Humidity control of the Permadry™ system

The Permadry™ system with its 2-pan water system guarantees a maximum humidity of up to 95 % r.H. in the inner chamber, which remains condensation-free. This performance assumes an average ambient temperature of 22 ±3 °C / 71.6 ±5.4 °F and a working temperature in the inner chamber of 37 °C /
98.6 °F. You can increase or decrease humidity slightly if required. To configure the humidity control of the Permadry™ system, go to Menu > Humidity control
08.03.2013 05:05:06
Submenu “Humidity control”. The current setting is displayed. To change the setting, press the operating button.
Factory default setting: “Medium”.
Close Home
08.03.2013 05:05:06
Close Home
If the ambient temperature deviates by more than +/- 5 °C from the values recommended by the manufacturer, the conditions for maximum air humidity with condensation-free inner chamber are no longer guaranteed. Contact BINDER Service for assistance.
Submenu “Humidity control”. Select the desired setting
and press the operating button
CB (E6) 09/2013 Page 47/144
Fixed value
..\ Interior socket
Socket On
Socket Off

6.5 Turning on / off the interior socket voltage (with optional interior socket)

For chambers equipped with the optional interior socket (chap. 15.4) you can turn on and off the voltage of the interior socket.
To turn on or off the interior socket voltage, go to Menu > Interior socket (optional)
08.03.2013 05:05:06
Submenu “Interior socket”. Select the desired setting
and press the operating button “Socket On” = socket with activated voltage
“Socket Off” = socket voltage-free
Close Home
Go back to the initial view with “Home”.
This symbol on the controller display indicates that the interior socket is activated.

6.6 Performance during and after power failure

During a power failure, all controller functions are shut down. The gas inlet valves are closed so that no gas can escape into the ambient air. The zero-voltage relay alarm output (9) (chap. 11.6) is switched to alarm position for the whole duration of the power failure.
After the power returns, all functions return to the same status the chamber had before power failure. . The controller continues to function in the original operating mode it was in previously before the power failure occurred. In “fixed value” mode, the setpoints are immediately resumed. The power failure and power return are noted in the event list.
If the CO unit operates in “fixed value” operation mode with the setpoints already entered. All setpoint values remain in memory.
If the temperature or concentration of CO limits during power failure, confirm the alarms with the RESET button as soon as the correct values are reached again (chap. 11.4).
incubator had been running in sterilization mode, the sterilization process is cancelled and the
2
, and O2 (unit with O2 control) have dropped below the alarm
2
CB (E6) 09/2013 Page 48/144
Fixed value
..\ System information
Chamber type: CB
Chamber name: CB_O2_E6
Serial number: 13-00000
Special application number: 11-564
Date of production (YY/MM/DD): 2013/01/01
Parameter version: 511B-0004-0015
Firmware version (1): 521C-0001-0023
Firmware version (2): 521B-0005-0017
Fixed value
..\ Service contact
Service hotline
International:
+49 7462 2005 555
USA Toll Free:
+ 1 866 885 9794
or
+ 1 631 224 4340
CIS:
+ 7 495 988 1517
service@binder-world.com
www.binder-world.com

6.7 Information

You access chamber information like the chamber type, serial no., firmware version etc. To display the system information, go to Menu > System information
08.03.2013 05:05:06
Submenu “System information” (sample values).
Close Home
To display the BINDER Service contact data, go to Menu > Service contact
08.03.2013 05:05:06
Submenu “Service contact”.
Close Home
Further information window can be accessed under Menu > Settings > Network settings > Show
network settings (chap. 9.9) and – for Service purpose – under Menu > Settings > Chamber configuration (chap. 9.12).
CB (E6) 09/2013 Page 49/144

7. Setpoint entry

7.1 Setting ranges

Temperature 7 °C / 12.6 °F above ambient up to 60 °C / 140 °F
CO2
O2 (unit with O2 control)
0 vol.-% up to 20 vol.- %
0.2 vol.-% up to 95 vol.-%
When changing the temperature setpoint, check the setting of the overtemperature safety controller class 3.1 (chap. 14.2).
With setpoint type “Limit”, adapt the safety controller always when changing the temperature setpoint.
When setting a lower temperature setpoint, in order to save time, we recommend cooling down the unit by turning it off and opening both doors of the unit.
When setting a lower CO of the unit for this purpose.
setpoint, the CO2 gas must be able to escape first. Open both doors
2

7.2 Note when setting high gas concentrations

Notes on handling carbon dioxide (CO2)
Carbon dioxide (CO and therefore practically imperceptible. Vent out any CO or a suitable connection to an exhaust system. We recommend installing a CO
) in high concentrations is hazardous to health. It is colorless and almost odorless
2
gas that may escape via good room ventilation
2
warning system.
2
WARNING
High concentration of CO2 (> 4 Vol.-%). Danger of death by suffocation. Danger of poisoning.
Do NOT set up units in non-ventilated recesses.
Ensure technical ventilation measures. Observe the relevant regulations for handling CO
.
2
If CO2 is released, leave the area und inform the security service or fire department.
Unit with O
Oxygen (O
control: Notes on handling oxygen (O2)
2
) is colorless and almost odorless and therefore practically imperceptible. It promotes burns,
2
which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes and hair. O
is heavier than air and may accumulate in low-lying areas.
2
CB (E6) 09/2013 Page 50/144
WARNING
High concentration of O2 (> 21 % O2). Fire and explosion hazard through contact of combustible materials with O
.
2
Risk of burns and other injuries.
Do NOT set up units in non-ventilated recesses
Ensure technical ventilation measures Observe the relevant regulations for handling O
.
2
Take appropriate measures to prevent oxygen enrichment and fire and explosion hazards in areas where oxygen enrichment is possible.
Observe the general information for safe handling of oxygen (chap. 1.6).
Unit with O
Nitrogen (N therefore practically imperceptible. Any N
control: Notes on handling nitrogen (N2)
2
) in high concentrations is hazardous to health. It is colorless and almost odorless and
2
gas that may escape must be safely led out via good room
2
ventilation or a suitable connection to an exhaust system.
High concentration of N2. Risk of death by suffocation.
Do NOT set up units in non-ventilated recesses
Ensure technical ventilation measures Observe the relevant regulations for handling N
WARNING
.
2
CB (E6) 09/2013 Page 51/144
Fixed value
..\ Quick menu
Measurement chart
Active alarms
Temperature setpoint
CO2 setpoint
O2 setpoint
Safety controller setpoint
Hot-air sterilization
3
[°C]

7.3 Entering the setpoints via “quick menu”

To enter setpoints via quick menu, go to Quick menu.
08.03.2013 05:05:06
“Quick menu”. (item “O2 setpoint” only coming with
control)
2
Close Home
When trying to enter a setpoint in the „Controller off“ operating mode, a notification window shows “Controller mode is OFF!”. Press the operating button to confirm with “Ok” and change the operating mode to “Fixed value” (chap. 6.2.1).
unit with O Select the desired parameter
and press the operating button.
Temperature setting
To enter the temperature setpoint, go to Quick menu > Temperature setpoint
Fixed value
08.03.2013 05:05:06
..\ Temperature setpoint
Entry menu “Temperature setpoint”.
End Ok 0 1 2 4 5 6 7 8 9 Ins
3
Select each number with the operating button and press the operating button to confirm.
Setting range: 20 °C / 68 °F up to 60 °C / 140 °F. Press the “Ok” button to confirm.
Close Ok Home
When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.
Go back to the initial view with “Home” or enter the CO
concentration.
2
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Fixed value
..\ CO2 setpoint
5
[Vol.-%]
Fixed value
..\ O2 setpoint
5
[Vol.-%]
Setting the CO
To enter the CO
concentration
2
setpoint, go to Quick menu > CO2 setpoint
2
08.03.2013 05:05:06
Entry menu “CO2 setpoint”.
End Ok 0 1 2 3 4
6 7 8 9 Ins Del
5
Select each number with the operating button and press the operating button to confirm.
Setting range: 0 vol.-% up to 20 vol.-%. Press the “Ok” button to confirm.
Close Ok Home
When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.
Go back to the initial view with “Home” or enter the O
concentration (unit with O2 control).
2
Setting the O
To enter the O
concentration (unit with O2 control)
2
setpoint, go to Quick menu > O2 setpoint
2
08.03.2013 05:05:06
Entry menu “O2 setpoint”.
End Ok 0 1 2 3 4
6 7 8 9 Ins Del
5
Select each number with the operating button and press the operating button to confirm.
Setting range: 0.2 vol.-% up to 95 vol.-%. Press the “Ok” button to confirm.
Close Ok Home
When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.
Go back to the initial view with “Home”.
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Fixed value
..\ Setpoints
Temperature setpoint
CO2 setpoint
O2 setpoint
Fixed value
..\ Temperature setpoint
3
[°C]

7.4 Entering the setpoints via general menu

To enter setpoints via general menu, go to Menu > Setpoints
08.03.2013 05:05:06
Submenu “Setpoints”.
2
Close Home
When trying to enter a setpoint in the „Controller off“ operating mode, a notification window shows “Controller mode is OFF!”. Press the operating button to confirm with “Ok” and change the operating mode to “Fixed value” (chap. 6.2.1).
(item “O2 setpoint” only coming with unit with O control)
Select the desired parameter and press the operating button.
Temperature setting
To enter the temperature setpoint, go to Menu > Setpoints > Temperature setpoint
08.03.2013 05:05:06
Entry menu “Temperature setpoint”.
End Ok 0 1 2 4 5 6 7 8 9 Ins
3
Select each number with the operating button and press the operating button to confirm.
Setting range: 20 °C / 68 °F up to 60 °C / 140 °F. Press the “Ok” button to confirm.
Close Ok Home
When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.
Go back to the initial view with “Home” or enter the CO
concentration.
2
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Fixed value
..\ CO2 setpoint
5
[Vol.-%]
2
[Vol.-%]
Setting the CO
To enter the CO
concentration
2
setpoint, go to Menu > Setpoints > CO2 setpoint
2
08.03.2013 05:05:06
Entry menu “CO2 setpoint”.
End Ok 0 1 2 3 4
6 7 8 9 Ins Del
5
Select each number with the operating button and press the operating button to confirm.
Setting range: 0 vol.-% up to 20 vol.-%. Press the “Ok” button to confirm.
Close Ok Home
When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.
Go back to the initial view with “Home” or enter the O
concentration (unit with O2 control).
2
Setting the O
To enter the O
Fixed value
concentration (unit with O2 control)
2
setpoint, go to Menu > Setpoints > O2 setpoint
2
08.03.2013 05:05:06
..\ O2 setpoint
Entry menu “O2 setpoint”.
Pos1 End Ok 0 1
3 4 5 6 7 8 9
2
Select each number with the operating button and press the operating button to confirm.
Setting range: 0.2 vol.-% up to 95 vol.-%. Press the “Ok” button to confirm.
Close Ok Home
When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.
Go back to the initial view with “Home”.
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Fixed value
..\ User
Key lock
Show event list
Close
Home
Fixed value
..\ Key lock
Key lock On
Automatic key lock
Password

8. Key lock

The key lock function serves to block the access to the controller. When the “key lock” function is activated, the controller remains in the actual view and can only be changed when entering the current password.
To configure the key lock function, go to User > Key lock
08.03.2013 05:05:06
“User” menu. Select “Key lock”
and press the operating button.
08.03.2013 05:05:06
Submenu “Key lock”. Select the desired function
and press the operating button.
Close Home
Key lock On The key lock is directly activated
Automatic key lock The key lock is activated automatically after a defined waiting time
Password Change password for unlocking. Factory setting: 0000
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Fixed value
..\ Key lock
Key lock On
Automatic key lock
Password
Fixed value
..\ Automatic key lock
Automatic key lock
Waiting time [min]

8.1 Directly activating the key lock

To directly activate the key lock, go to User > Key lock > Key lock On
08.03.2013 05:05:06
Submenu “Key lock”. Select “Key lock On” to activate the key lock function
immediately and press the operating button.
Close Home
This symbol on the controller displays indicates that the “key lock” function is activated.
The controller remains in the initial view and may be operated only after entering the current password.
Enter password
08.03.2013 05:05:06
..\ Key lock password
Ok: A B C D E F
1 2 3 4 5 6 7 8 9
0
Entry menu “Key lock password”. Enter the password with the operating button.
Factory setting is 0000 Press the “Ok” button to confirm.
Close Ok Home

8.2 Automatic key lock

To configure the automatic key lock, go to User > Key lock > Automatic key lock
08.03.2013 05:05:06
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Submenu “Automatic Key lock”. Select the desired function
and press the operating button.
Close Home
1
Fixed value
..\ Automatic key lock On/Off
Automatic key lock : Off
Fixed value
Setpoint
Actual value
37.0
37.2
5.0
5.0
85.5
Menu
Under “Waiting time [min]” you can enter the waiting time, after which the key lock will be automatically activated. This time starts running off after the last entry to the controller. To enter it, go to
User > Key lock > Automatic key lock > Waiting time [min]
Enter wait time [min]
..\ User\Waiting time
Pos1 End Ok 0
Close Ok Home
To activate the automatic key lock function with the pre-configured waiting time, select
User > Key lock > Automatic key lock > Automatic key lock
1
08.03.2013 05:05:06
2 3 4 5 6 7 8 9
08.03.2013 05:05:06
Entry menu “Waiting time”. Enter the desired interval with the operating button.
This interval starts running off after the last action on the controller. If the automatic key lock function is enabled, it will become active after this time.
Factory setting: 1 minute. Press the “Ok” button to confirm.
Submenu “Automatic Key lock On/Off”. The current setting is displayed. To change the setting, press the operating button.
“Automatic Key lock : On” = Automatic key lock function activated. The time set under “Waiting time” begins running off.
“Automatic Key lock : Off” = Automatic key lock function deactivated
Close Home
Now the waiting time starts running off. Go back to the initial view with “Home”.
08.03.2013 05:05:06
Temperature [°C]
CO2 [Vol.-%]
Humidity [%rH]
User Quick menu
The controller remains in the initial view and may be operated only after entering the current password. After further entries to the controller, the waiting period begins running again, since the automatic keylock
function remains active until turning it off manually.
Initial view. As soon as the waiting period has expired, the “key
lock” symbol is displayed.
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Fixed value
..\ Key lock
Key lock On
Automatic key lock
Password
Fixed value
..\ Key lock \ Change password
Do not change
Change password
This symbol on the controller displays indicates that the “key lock” function is activated.

8.3 Changing the password for unlocking the key lock

To change the password for unlocking the key lock, go to User > Key lock > Password
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Submenu “Key lock”. Select „Password“
and press the operating button.
Close Home
A security question is displayed:
08.03.2013 05:05:06
Close Home
Change password
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..\ Key lock password
Ok: A B C D E F
1 2 3 4 5 6 7 8 9
0
Close Ok Home
Submenu “Change password”. To change the password,
select “Change password” and press the operating button.
Entry menu “Key lock password”. Enter the desired password with the operating
button. Factory setting is 0000 Press the “Ok” button to confirm.
Keep well in mind any password modification. Without the correct password, unlocking the key lock is impossible.
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Fixed value
..\ Settings
Setup wizard
Date and time
Sprache, Language, Langue, Idioma, Lingua
Display brightness
Temperature unit
Recording rate
Factory reset
Network settings
Fixed value
..\ Settings
MAC address
Chamber configuration
RS 422 address (optional)

9. General controller settings

In the "Settings" submenu, you can enter the date and time, select the language for the menus and the desired temperature unit, perform the configuration for the controller’s communication functions, and reset the controller to factory settings.
To access the "Settings" submenu, go to Menu > Settings
08.03.2013 05:05:06
Submenu “Settings”. Turn the operating button to see additional menu
items.
Close Home
08.03.2013 05:05:06
Submenu “Settings” (next page).
Close Home
Setup wizard Chap. 9.1
Date and time Setting date and time, chap. 9.2
Sprache, Language, Langue, Idioma, Lingua
Selecting the controller’s menu language, chap. 9.3
Display brightness Adjusting display brightness by turning the operating button
Temperature unit Selecting the temperature unit, chap.9.5
Recording rate Defining the recording rate for data storage, chap. 9.6
Factory reset Factory reset, chap. 9.7
Network settings Network configuration, chap. 9.8
MAC address Displaying the MAC address, chap. 9.10
Chamber configuration
RS 422 address (optional)
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Menu for Service purpose – Entering chamber data like the serial no., service information
Setting the RS 422 unit address(with option RS422 interface), chap.
9.11
Fixed value
..\ Date and time
Set date
Set time

9.1 Setup wizard

The setup wizard will guide you sequentially through the important menus to configure your chamber
Menu language
Device name
Date and time
IP address
Subnet mask
Network name
Gateway
DNS 1
DNS 2
Then the controller returns to the initial view.
Use the setup wizard only if you want to enter all the requested information, as no item can be skipped.
You can configure the network settings (IP address, and the following) only if the DHCP status is Off, otherwise the DHCP server would assign the network configuration.
If you try configuring any network settings (i.e., when reaching item “IP address” in the setup wizard) while DHCP is enabled, the message "DHCP enabled!” is shown. After confirming with Ok the Setup wizard is cancelled and the controller returns to the initial display. Any settings made so far remain valid.

9.2 Date and time settings

To access the date and time settings, go to Menu > Settings > Date and time
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Submenu “Date and time”. Select the desired function
and press the operating button.
Close Home
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Fixed value
0
8.03.2013
0
5:05:06
1 2 3 4 5 6 7 8 9
Function “Set date”
08.03.2013 05:05:06
..\ Select date (DD.MM.YYYY)
Ins Pos1 End Ok
1 2 3 4 5 6 7 8 9
0
Entry menu “Select date”. The current date is shown. If it is incorrect, enter the
correct date with the operating button. Press the “Ok” button to confirm.
Close Ok Home
Function “Set time”
Fixed value
08.03.2013 05:05:06
..\ Set time (HH:MM:SS)
Ins Pos1 End Ok
0
Entry menu “Set time”. The current time is shown. If it is incorrect, enter the
correct time with the operating button. Press the “Ok” button to confirm.
Close Ok Home
There is no automatic switch to local light-saving times because this could lead to problems with data seeming to be missing or being overwritten in the data base.
Go back to the “Settings” menu with “Close” or to the initial view with “Home”.
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Fixed value
..\ Sprache, Language, Langue, Idioma, Lingua
Deutsch
English
Français
Español
Italiano
Fixed value
..\ Display brightness

9.3 Selecting the menu language of the T4.12 controller

The T4.12 chamber controller communicates via a comprehensible menu navigation in plain text in a selectable language.
To select the desired menu language, go to
Menu > Settings > Sprache, Language, Langue, Idioma, Lingua
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Submenu “Language”. Select the desired language
and press the operating button.
Close Home
Go back to the “Settings” menu with “Close” or to the initial view with “Home”.

9.4 Setting display brightness

To select the display brightness, go to Menu > Settings > Display brightness
08.03.2013 05:05:06
Submenu “Display brightness”. Select the desired setting by turning the
operating button. Confirm by selecting “Save”.
Close Save Home
Go back to the “Settings” menu with “Close” or confirm the change with “Save”: The controller returns to the initial view.
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Fixed value
..\ Temperature unit
Celsius [°C]
Fahrenheit [°F]
Kelvin [K]
6
0

9.5 Changing the temperature unit

To select the temperature unit, go to Menu > Settings > Temperature unit
08.03.2013 05:05:06
Submenu “Temperature unit”. Select the desired temperature unit”
and press the operating button.
Close Home
Go back to the “Settings” menu with “Close” or to the initial view with “Home”.

9.6 Defining the data recording rate

To define the recording rate for data storage on the SD card, go to Menu > Settings > Recording rate
Fixed value
..\ Recording rate (seconds)
Ok 0 1 2 3 4 5
6
Close Ok Home
Go back to the “Settings” menu with “Close” or to the initial view with “Home”.
Note:
08.03.2013 05:05:06
7 8 9 . – Del Ins
Entry menu “Recording rate”. The current recording rate is shown. Enter the
desired value with the operating button. Shortest possible value: 60 seconds. Press the “Ok” button to confirm.
After a period of 3 years, the controller starts overwriting the oldest values on the storage medium. This is independent of the selected storage interval and the actual operating time of the chamber. In any case, the data can be read out at any time using the function “Export to USB drive” (chap. 10.1) and stored externally.
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Fixed value
..\ Factory reset
Do not reset
Really reset
Fixed value
..\ Network settings
Show network settings
DHCP On/Off
IP address
Subnet mask
Chamber name
Network name
Gateway
DNS1

9.7 Factory reset

The “factory reset” function allows resetting the controller configuration to the factory settings.
Risk of data loss! When resetting to factory settings, all controller settings will be deleted. The
event list and stored measuring data are NOT affected.
To access the “Factory reset” function, go to Menu > Settings > Factory reset
08.03.2013 05:05:06
Submenu “Factory reset”. This a security question. Select the desired function
and press the operating button.
Close Home
Go back to the “Settings” menu with “Close” or to the initial view with “Home”.

9.8 Network configuration

The settings of this submenu are required for networking chambers with an Ethernet interface, e.g. to connect them with BINDER’s communication software APT-COM™ 3 DataControlSystem.
You can display the chamber‘s IP address that has been assigned by your DHCP server or manually assign the IP address. All necessary configurations for networking the chamber are available in this menu.
To view and configure the network settings, go to Menu > Settings > Network settings
To configure the network settings, the DHCP state must be set to off. Otherwise, the DHCP­server would assign the network configuration.
If you try to configure the network settings with enabled DHCP state, the message “DHCP enabled!” is shown. Confirm with „Ok“ to return to the “Network settings” menu.
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Close Home
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Submenu “Network settings”. Turn the operating button to see additional menu
items.
Fixed value
..\ Network settings
DNS2
Fixed value
..\ DHCP On/Off
DHCP : On
08.03.2013 05:05:06
Submenu “Network settings” (next page).
Close Home
Show network settings Overview of the entire network configuration
DHCP On/Off
IP address
Subnet mask
Chamber name
Network name
Gateway
DNS1
Switching on and off the DHCP state
Entering the desired IP address
Entering the subnet mask number
Entering the name of the CO
Entering the network name
Entering the gateway number
Entering the DNS1 number
DNS2 Entering the DNS2 number
Set the DHCP State on/off:
22.08.2012 05:05:06
Submenu “DHCP On/Off”. The current DHCP state is displayed. Press the
operating button to change it. Then the new DHCP state is displayed “DHCP : On” = DHCP state activated
“DHCP : Off” = DHCP state deactivated
incubator
2
Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.
CB (E6) 09/2013 Page 66/144
Close Home
You can only configure the following network settings if the DHCP state is set to “Off”.
1
92.168.0.100
Fixed value
..\ Subnet mask (n.n.n.n.)
2
55.255.255.0
Fixed value
..\ Chamber name
C
B_E6
Enter the IP address:
Fixed value
08.03.2013 05:05:06
..\ IP address (n.n.n.n.)
Entry menu “IP address”
Ins Pos1 End Ok 0
2 3 4 5 6 7 8 9 .
1
(example value) Enter the desired IP address with the operating
button. Press the “Ok” button to confirm.
Close Ok Home
Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.
Enter the subnet mask:
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Entry menu “Subnet mask”
Pos1 End Ok 0 1
3 4 5 6 7 8 9 , -
2
(example value) Enter the desired net mask with the operating
button. Press the “Ok” button to confirm.
Close Ok Home
Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.
Enter the chamber name:
08.03.2013 05:05:06
End Ok _ : A B
D E F G H I J K
C
Entry menu “Chamber name”. Enter the desired chamber name with the operating
button. Press the “Ok” button to confirm.
Close Ok Home
Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.
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C
B_E6
Fixed value
..\ Gateway (n.n.n.n.)
1
92.168.0.1
Fixed value
..\ DNS 1 (n.n.n.n.)
1
92.168.0.1
Enter the network name:
Fixed value
08.03.2013 05:05:06
..\ Network name
End Ok _ : A B
D E F G H I J K
C
Entry menu “Network name”. Enter the desired network name with the operating
button. Press the “Ok” button to confirm.
Close Ok Home
Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.
Enter the default gateway:
08.03.2013 05:05:06
Pos1 End Ok 0
2 3 4 5 6 7 8 9 ,
1
Entry menu “Gateway” (example value)
Enter the desired gateway with the operating button. Press the “Ok” button to confirm.
Close Ok Home
Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.
Enter the DNS 1 or DNS 2:
08.03.2013 05:05:06
Ins Pos1 End Ok 0
2 3 4 5 6 7 8 9 ,
1
Entry menu “DNS 1” or “DNS 2” (example value)
Enter the desired number with the operating button. Press the “Ok” button to confirm.
Close Ok Home
Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.
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Fixed value
..\ Show network settings
DHCP
Off
IP address
192.168.0.100
Net mask
255.255.255.0
Gateway
192.168.0.1
DNS1
192.168.0.1
DNS2
0.0.0.0
Chamber name
CB_E6
BIOS name
CB_E6
Close
00-04-A3-B1-00-B9

9.9 Display of the network configuration

To access the overview of the complete network configuration, go to
Menu > Settings > Network settings > Show network settings
08.03.2013 05:05:06
Overview of the network configuration (sample values)
Home
Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.

9.10 MAC Address

To identify the chamber in the Ethernet network you can display the chamber’s MAC address. To display the chamber’s MAC address, go to Menu > Settings > MAC address
Fixed value
..\ MAC address
Close Home
Go back to the “Settings” menu with “Close” or to the initial view with “Home”.
08.03.2013 05:05:06
Submenu “MAC address” (example value)
The MAC address is displayed.
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Fixed value
..\ RS 422 address
1

9.11 RS 422 address (with optional RS 422 interface)

For chambers equipped with the optional RS 422 interface, the RS 422 address serves to identify the chamber in a network and to establish communication with the optional BINDER communication software APT-COM™ 3 DataControlSystem. The factory default setting is “1”.
To enter the RS 422 unit address, go to Menu > Settings > RS 422 address (optional)
08.03.2013 05:05:06
Pos1 End Ok 0
2 3 4 5 6 7 8 9
1
Close Ok Home
Entry menu “RS 422 address”. Enter the desired address (1 up to 254) with the
operating button. Press the “Ok” button to confirm.
Go back to the “Settings” menu with “Close” or to the initial view with “Home”.
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Fixed value
..\ Chamber configuration
Enter serial number
Enter special application number
Service window 1
Service window 2
Fan speed setpoint
Door heating offset
Humidity control offset
Error memory
Fixed value
..\ Chamber configuration
Firmware update

9.12 Display and entry of the device configuration – for service purpose

Information about the chamber, such as chamber type, name, serial number, firmware version, etc. can be viewed under Menu > System information (chap. 6.7).
To access the device configuration menu, go to Menu > Settings > Chamber configuration This menu is password protected and only intended for Service purpose.
08.03.2013 05:05:06
Submenu “Chamber configuration”.
Close Home
Turn the operating button to see additional menu items.
08.03.2013 05:05:06
Submenu “Chamber configuration” (next page).
Close Home
After selecting the desired function, the service password is requested in order to deny unauthorized access.
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Fixed value
..\ Import/Export to USB drive
Export to USB drive
Import from USB drive
Fixed value
..\ Export to USB drive
Chamber status
Measurement values
Event list
Configuration
Adjustment
Program parameters
System information
All

10. Data transfer via USB interface

The USB port is located in the instrument box. To access the submenus for data transfer, go to Menu > Import/Export
08.03.2013 05:05:06
Submenu “Import/Export to USB drive”. Select the desired type of communication via USB
port (data export or import) and press the operating button.
Close Home
It is possible that some USB devices are not recognized due to compatibility issues. In this case, please use a USB storage device from a different manufacturer.

10.1 Exporting data to USB drive

Insert the USB stick or the plug of your USB drive into the USB port in the instrument box. To configure data export to USB media, go to Menu > Import/Export > Export to USB drive
08.03.2013 05:05:06
Submenu “Export to USB drive”.
Close Home
Select the desired data type and press the operating button. Data is written to the connected media.
Chamber status Actual chamber status, including operating mode, setpoints etc.
Measurement values Measured data
Event list List of status information and errors (see chap. 12)
Configuration (Service only)
Adjustment Adjustment data
Program parameters (Service only)
System information (Service only)
All All data,
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Fixed value
..\ Import from USB drive
Configuration
If no USB device has been connected, the message “Insert USB device” is displayed. It disappears after inserting the USB stick or the plug of your USB drive into the USB port in the instrument box.
This symbol on the controller displays indicates that data are being transmitted via the USB port.

10.2 Importing data from USB drive

Insert the USB stick or the plug of your USB drive into the USB port in the instrument box. To configure data import from USB media, go to Menu > Import/Export > Import from USB drive
08.03.2013 05:05:06
Close Home
Configuration
(Service only)
If no USB device has been connected, the message “Insert USB device” is displayed. It disappears after inserting the USB stick or the plug of your USB drive into the USB port in the instrument box.
This symbol on the controller displays indicates that data are being transmitted via the USB port.
Submenu “Import from USB drive”. Select “Configuration”
and press the operating button. Data is read from the connected media.
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Icon
Signification
Icon
Signification
Icon
Notifying message
Signification
Icon
Alarm message
Signification

11. Notifications and Alarms

11.1 Notifications overview

O
control deactivated. No O2
Fixed value operation
2/N2
concentration alarms will occur. (unit with O
control)
2
Heating active
Key lock activated
Copying data via USB
Interior socket turned on (with option interior socket)
CO2 pressure alarm deactivated
O2 pressure alarm deactivated (unit with O
control)
2
N2 pressure alarm deactivated (unit with O
control)
2
DO NOT OPEN THE DOOR !
STERILIZATION FINISHED
STERILIZATION ABORTED
Hot air sterilization running
Hot air sterilization successful The inner chamber and parts inside can still be hot. Do not touch.
Hot air sterilization cancelled
The inner chamber and parts inside can still be hot. Do not touch.

11.2 Alarm messages overview

Safety controller overtemperature
Temp. range
CO2 range
O2 range
Overtemperature safety controller alarm (class 3.1): selected value of the safety controller exceeded.
Having reached the setpoint, the temperature deviates by more than the set tolerance range value (chap. 11.5.6) and longer than 10 min. from the setpoint or the temperature doesn’t reach the tolerance range within 3 hours from turning on the unit or closing the door
Having reached the setpoint, the CO
concentration deviates by more than
2
the set tolerance range value (chap. 11.5.6) and longer than 10 min. from the setpoint or the CO
concentration doesn’t reach the tolerance range
2
within 3 hours from turning on the unit or closing the door
Having reached the setpoint, the O
concentration deviates by more than
2
the set tolerance range value (chap. 11.5.6) and longer than 10 min. from the setpoint or the O within 3 hours from turning on the unit or closing the door (unit with O
concentration doesn’t reach the tolerance range
2
2
control)
CB (E6) 09/2013 Page 74/144
Icon
Alarm message
Signification
After the humidity was situated within the defined tolerance range (chap.
Humidity range
11.5.6) it deviates longer than 10 min. from the tolerance range or the humidity doesn’t reach the tolerance range within 3 hours from turning on the unit or closing the door
Door open
Door is open for more than the set alarm delay time (chap. 11.5.5, factory setting: 1 minute). Close the door.
Low pressure CO2 Low CO2 outlet pressure (< 0.3 bar)
Low pressure CO2 Low O2 outlet pressure (< 0.3 bar) (unit with O2 control)
Low pressure N2 Low N2 outlet pressure (< 0.3 bar) (unit with O2 control)
CO2 sensor defective
O2 sensor defective
Inform service ­adjust CO2 sensor
sensor defective. Contact BINDER Service.
CO
2
sensor defective. Contact BINDER Service.
O
2
sensor adjustment required. Contact BINDER Service.
CO
2
You can activate / deactivate the buzzer in the “Alarms” submenu (chap. 11.5.3). With an activated buzzer there is an audible alert with an alarm. You can reset it in the “Alarms”
submenu for alarm acknowledgement pressing the “Reset” button (chap. 11.4). The alarm symbol will only disappear when the cause of the alarm has been remedied.
The zero-voltage relay alarm contact (chap. 11.6) is activated in case of the alarm messages “door open” and deviations of temperature and gas concentrations) as well as in case of a power failure and turning off the incubator at its main power switch.
For appropriate actions in the event of an alarm, please refer to chap. 21 “Troubleshooting”.
CB (E6) 09/2013 Page 75/144

11.3 Alarm status

An alarm message can appear in 3 different states:
“Set”
Active alarm.
The corresponding alarm icon is displayed in the initial view The buzzer sounds (if activated).
The “Info” button in the initial view leads to the “Alarms” submenu for alarm acknowledgement.
Press the “Reset” button in the “Alarms” submenu for alarm acknowledgement to mute the buzzer and
confirm the alarm.
“Acknowledged”
Active alarm.
The alarm was acknowledged. The buzzer is off.
The alarm cause is still valid. Therefore, the alarm icon remains displayed in the initial view.
The alarm message figures in the list of active alarms.
“Cleared”
The alarm cause has disappeared.
The alarm icon is not displayed any longer
The alarm message has disappeared from the list of active alarms.
The alarm message remains in the “Event list” for information.
CB (E6) 09/2013 Page 76/144
Fixed value
Setpoint
Actual value
37.0
39.2
5.0
5.0
85.5
Info
Alarm acknowledgement
../Home/Alarms
Safety controller overtemperature

11.4 Confirming an active “set” alarm

08.03.2013 05:05:06
Temperature [°C]
CO2 [Vol.-%]
Humidity [%]
User
08.03.2013 05:05:06
Initial view with overtemperature safety controller alarm.
The buzzer sounds (if not deactivated previously). Press the “Info” button.
Menu
Submenu “Alarms” for alarm acknowledgement.
Close Reset Home
Press the “Reset” button to confirm the alarm. If there is another active “set” alarm, you can also confirm it with the “Reset” button. After confirming all active “set” alarms, the buzzer is off, and the initial view is displayed.
As long as an alarm is still active, i.e. as long as the alarm cause is still valid, the alarm icon continues to be displayed in the initial view.
CB (E6) 09/2013 Page 77/144
Fixed value
..\ Alarm
Active alarms
History
Buzzer test
Buzzer On/Off
Alarms On/Off
Door alarm delay
Tolerance ranges
Fixed value
..\ Active alarms
Safety controller overtemperature

11.5 Alarm configuration and overview

To access the alarm lists and configuration menu, go to Menu > Alarms
08.03.2013 05:05:06
Submenu “Alarm”. Select the desired function
and press the operating button.
Close Home
Active Alarms List of the alarms with status “set” or “confirmed”.
History List of all alarms (status “set” or “confirmed ” or “cleared”)
Buzzer test Testing the alarm buzzer, chap. 11.5.3
Buzzer On/Off Activating / deactivating the alarm buzzer, chap. 11.5.3
Alarms On/Off
Activating / deactivating the alarm functions. Off: Alarm buzzer off, no alarm icons displayed.
Door alarm delay Entering the delay time of the door alarm
Tolerance ranges
Defining the tolerance ranges and alarm delay times for the individual parameters

11.5.1 List of active alarms

To access the overview list of active alarms, go to Menu > Alarms > Active alarms
08.03.2013 05:05:06
Submenu “Active alarms“. All active alarms with status “set”
are listed.
or “acknowledged”,
Close Home
If no alarm is active (“set” or “acknowledged”), no message will be displayed in this window.
CB (E6) 09/2013 Page 78/144
Fixed value
..\ History
08.03.2013 : Messages of indicated day
14:39:48
Alarm set Safety controller overtemperature
15:03:22
Alarm cleared Safety controller overtemperature
0
8.03.2013
Fixed value
..\ Event list
08.03.2013 : Messages of indicated day
14:17:20
Temperature setpoint 35.00
14:35:12
Temperature setpoint 37.00
14:39:48
Alarm set Safety controller overtemperature
14:40:19
Alarm acknowledged Safety controller overtemp.
15:03:22
Alarm cleared Safety controller overtemperature

11.5.2 History – list of all alarms

To access the overview list of all alarms, go to Menu > Alarms > History This list indicates the moment when an alarm was set and when cleared.
08.03.2013 15:05:06
Submenu “History” (example). The list shows when the alarms of the current day
were triggered and when cleared. The most recent message appears at the end of the list.
The information that an alarm has been acknowledged is shown in the event list.
Close Home
When there is more information than one page, you can scroll the list in both directions with the operating button.
To select a different date, select “Messages of indicated day” and press the operating button. You can enter the desired date through an entry menu.
Fixed value
08.03.2013 05:05:06
..\ Select date (DD.MM.YYYY)
Ins Pos1 End Ok
1 2 3 4 5 6 7 8 9
0
Entry menu “Select date”. The current date is shown. Enter the desired date
with the operating button. Press the “Ok” button to confirm.
Close Ok Home
The alarm list of the selected date is displayed. The entire sequence of the alarm events (set – acknowledged – cleared) is shown in the event list (chap.
12). To access the event list, go to Menu > Event list or User > View event list
08.03.2013 15:05:06
Close Home
CB (E6) 09/2013 Page 79/144
Submenu “Event list” (example). The events and alarm messages of the current day
are displayed. The most recent message appears at the end of the list.
Fixed value
..\ Buzzer test
Buzzer test: Off
Fixed value
..\ Buzzer On/Off
Buzzer activation : On
Fixed value
..\ Alarm functions On/Off
Alarm functions: On

11.5.3 Activating, deactivating, and testing the alarm buzzer

Alarm buzzer test
To access the alarm buzzer functional test, go to Menu > Alarms > Buzzer test
08.03.2013 05:05:06
Submenu “Buzzer test”. The current setting is displayed. Press the operating button to turn on or off the
buzzer for test purpose. The modified setting is displayed.
“Buzzer test: On” = Buzzer turns on “Buzzer test: Off” = Buzzer turns off
Close Home
When turned on, the alarm buzzer emits an intermittent signal. To turn this off, change the test function to “Buzzer test: Off”.
Activating / deactivating the alarm buzzer
To activate or deactivate the alarm buzzer, go to Menu > Alarms > Buzzer On/Off
08.03.2013 05:05:06
Submenu “Buzzer On/Off”. The current setting is displayed. To change the setting, press the operating button. The modified setting is displayed.
“Buzzer activation: On” = Buzzer will turn on in the event of an alarm
“Buzzer activation: Off” = Buzzer is deactivated
Close Home

11.5.4 Activating / deactivating all alarm functions

To access the alarm function setting, go to Menu > Alarms > Alarms On/Off
08.03.2013 05:05:06
Close Home
CB (E6) 09/2013 Page 80/144
Submenu “Alarm functions On/Off”. The current setting is displayed. To change the setting, press the operating button. The modified setting is displayed.
“Alarm functions: On” = The alarm functions of the controller are active
“Alarm functions: Off” = All alarm functions are deactivated
..\ Door alarm delay
1
Fixed value
..\ Tolerance ranges
Temperature
CO2
Humidity
O2 Service settings

11.5.5 Setting the delay time after opening the door

To enter the delay time for the door open alarm, go to Menu > Alarms > Door alarm delay
Enter door alarm delay [min]
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Entry menu “Door alarm delay”
Pos1 End Ok 0
2 3 4 5 6 7 8 9
1
Enter the desired delay time in minutes with the operating button.
Setting range: 1 up to 999 Press the “Ok” button to confirm.
Close Ok Home
When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.

11.5.6 Tolerance ranges and alarm delay times

For temperature, CO2 , and O2 you can enter a value which defines a tolerance range around the
setpoint value. Example: Temperature setpoint: 37 °C, tolerance range value: ±2 °C, i.e. this defines a tolerance range from 35 °C up to 39 °C. If the actual value, after having reached the setpoint, deviates by more than the set tolerance range value and longer than 10 min. from the setpoint or the temperature doesn’t reach the tolerance range within 3 hours from turning on the unit or closing the door, tolerance alarm is triggered.
For humidity you can enter an upper and a lower tolerance range value. These are absolute values
which define the tolerance range. If the humidity, after being situated within the tolerance range, deviates longer than 10 min. from the tolerance range or if it doesn’t reach the tolerance range within 3 hours from turning on the unit or closing the door, tolerance range alarm is triggered.
To define the tolerance ranges and the alarm delay times for the individual parameters, go to
Menu > Alarms > Tolerance ranges
08.03.2013 05:05:06
Submenu “Tolerance ranges”. Select the desired parameter
and press the operating button.
Close Home
CB (E6) 09/2013 Page 81/144
Enter tolerance range
..\ Tolerance ranges \ Temperature
1
.0 [°C]
Enter alarm delay [min]
..\ Temperature alarm delay
1
Enter tolerance range
..\ Tolerance ranges \ CO2
1
.0 [Vol.-%]
Temperature
First you can enter the desired value of the temperature tolerance range:
08.03.2013 05:05:06
Pos1 End Ok 0
2 3 4 5 6 7 8 9
1
Entry menu “Temperature”. Enter the desired value of the temperature tolerance
range with the operating button. Press the “Ok” button to confirm.
Close Ok Home
Then you can enter the delay time for the temperature tolerance range alarm:
08.03.2013 05:05:06
Pos1 End Ok 0
2 3 4 5 6 7 8 9
1
Entry menu “Temperature alarm delay” Enter the desired delay time in minutes with the
operating button. Press the “Ok” button to confirm.
Close Ok Home
When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.
CO
2
First you can enter the desired value of the CO2 tolerance range:
08.03.2013 05:05:06
Entry menu “CO2”.
Pos1 End Ok 0
2 3 4 5 6 7 8 9
1
Enter the desired value of the CO with the operating button.
Setting range: 0 vol.-% up to 100 vol.-% Press the “Ok” button to confirm.
tolerance range
2
Close Ok Home
When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.
CB (E6) 09/2013 Page 82/144
..\ Tolerance ranges \ CO2
1
Enter Humidity Low Limit
..\ Tolerance ranges \ Humidity
1
.0 [%rH]
Enter Humidity High Limit
..\ Tolerance ranges \ Humidity
1
.0 [%rH]
Then you can enter the delay time for the CO
Enter alarm delay [min]
08.03.2013 05:05:06
Pos1 End Ok 0
2 3 4 5 6 7 8 9
1
tolerance range alarm:
2
Entry menu “CO2” Enter the desired delay time in minutes with the
operating button. Press the “Ok” button to confirm.
Close Ok Home
When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.
Humidity
First you can enter the lower value of the humidity tolerance range:
08.03.2013 05:05:06
Pos1 End Ok 0
2 3 4 5 6 7 8 9
1
Entry menu “Humidity”. Enter the desired value for the lower value
of the humidity tolerance range with the operating button.
Setting range: 0 % r.H. up to 100 % r.H. Press the “Ok” button to confirm.
Close Ok Home
When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.
Then you can enter the upper value of the humidity tolerance range:
08.03.2013 05:05:06
Pos1 End Ok 0
2 3 4 5 6 7 8 9
1
Entry menu “Humidity”. Enter the desired value for the upper value
of the humidity tolerance range with the operating button.
Setting range: 0 % r.H. up to 100 % r.H. Press the “Ok” button to confirm.
Close Ok Home
When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.
CB (E6) 09/2013 Page 83/144
..\ Tolerance ranges \ Humidity
1
Enter tolerance range
..\ Tolerance ranges \ O2
1
.0 [Vol.-%]
Enter alarm delay [min]
..\ Tolerance ranges \ O2
1
Then you can enter the delay time for the humidity tolerance range alarm:
Enter alarm delay [min]
Pos1 End Ok 0
1
08.03.2013 05:05:06
2 3 4 5 6 7 8 9
Entry menu “Humidity” Enter the desired delay time in minutes with the
operating button. Press the “Ok” button to confirm.
Close Ok Home
When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.
(unit with O2 control)
O
2
First you can enter the desired value of the CO
08.03.2013 05:05:06
tolerance range:
2
Entry menu “O2”.
Pos1 End Ok 0
2 3 4 5 6 7 8 9
1
Enter the desired value for the O with the operating button.
Setting range: 0 vol.-% up to 100 vol.-% Press the “Ok” button to confirm.
tolerance range
2
Close Ok Home
When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.
Then you can enter the delay time for the O
08.03.2013 05:05:06
Pos1 End Ok 0
2 3 4 5 6 7 8 9
1
tolerance range alarm:
2
Entry menu “O2” Enter the desired delay time in minutes with the
operating button. Press the “Ok” button to confirm.
Close Ok Home
When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.
Go back to the initial view with “Home”.
CB (E6) 09/2013 Page 84/144
Fixed value
..\ Tolerance ranges\ Service settings
Temperature alarm delay 2
CO2 alarm delay 2
Humidity alarm delay 2
O2 alarm delay 2
Service settings
08.03.2013 05:05:06
Submenu “Service settings”. Access to the following submenus is password-
protected and for service purpose only.
Close Home
CB (E6) 09/2013 Page 85/144
Displayed icon
Error / event when icon is displayed
Switching the alarm contact

11.6 Zero-voltage relay alarm output

The CO2 incubator is equipped with a zero-voltage relay output which permits the transmission of some alarm messages to a central monitoring system.
The connection is realized as a DIN socket (9) on the unit rear. A suitable DIN plug is enclosed.
Figure 16: Pin configuration of the DIN socket (9) on the unit rear
ALARM
Pin 1: Pole, Pin 2: Break relay, Pin 3: Make contact
In case there is no alarm, contact 1 closes with contact 3. Closing contact 1 with contact 2 switches the zero-voltage relay alarm output.
Maximum loading capacity of the switching contacts: 24V AC/DC – 2.5A
DANGER
Electrical hazard. Danger of death. Damage to switching contacts and connection socket.
Do NOT exceed the maximum switching load of 24V AC/DC – 2.5A. Do NOT connect any devices with a higher loading capacity.
The zero-voltage relay alarm output switches at the following events:
Power failure immediately Tolerance range alarm of
temperature, CO with O
control) or humidity
2
, O2 (unit
2
after 10 min from error
Door open for more than the set alarm delay time (chap.
11.5.5, factory setting: 1
after 10 minutes from door opening
minute)
In case of a tolerance range alarm or a door open alarm, the alarm message on the controller display remains on during the alarm transmission via the zero-voltage relay outputs.
As soon as the cause of the alarm is identified and resolved, you can reset the alarm transmission via the zero-voltage relay outputs together with the alarm message on the controller display by hitting the “RESET” key.
In case of a power failure, transmission of the alarm via zero-voltage relay outputs remains active for the duration of the power failure. After power returns, contact 1 closes automatically with contact 3.
When using the communication software APT-COM™ 3 DataControlSystem (option, chap.
15.1) via the Ethernet interface or optional RS 422 interface of the CO acquisition, only the alarm message is recorded in the APT-COM™ protocol.
Set the tolerance limits for limit alarms by APT-COM™ 3 separately in the APT-COM™ 3
CB (E6) 09/2013 Page 86/144
measuring window.
incubator for data
2
Fixed value
..\ Event list
08.03.2013
Messages of indicated day
14:17:20
Temperature setpoint 35.00
14:35:12
Temperature setpoint 37.00
14:39:48
Alarm set Safety controller overtemperature
14:40:19
Alarm acknowledged Safety controller overtemp.
15:03:22
Alarm cleared Safety controller overtemperature
Fixed value
..\ Select date
0
8.03.2013

12. Event list

The “Event list” displays status information and errors of the current day. You can also access the events of past days.
To access the event list, go to Menu > Event list or User > View event list
08.03.2013 15:05:18
Submenu “Event list” (example). The events of the current day are displayed. The
most recent message appears at the end of the list.
If you want to acknowledge an active alarm, proceed as described in chap. 11.4.
Close Reset Home
When there is more information than one page, you can scroll the event list in both directions with the operating button.
To select a different date, select “Messages of indicated day” and press the operating button. You can enter the desired date through an entry menu.
08.03.2013 05:05:06
Ins Pos1 End Ok
1 2 3 4 5 6 7 8 9
0
Entry menu “Select date”. The current date is shown. Enter the desired date
with the operating button. Press the “Ok” button to confirm.
Close Ok Home
The event list of the selected date is displayed.
CB (E6) 09/2013 Page 87/144
Fixed value
0:0 0:5 0:11 0:16 0:22
Fixed value
Settings
Sampling rate
Display range
Parameters
Fixed value
Sampling rate
Five seconds
Ten seconds
One minute
Five minutes
Ten minutes

13. Graphical display of the measured values

To access the graphical display, go to Menu > Measurement chart
08.03.2013 05:05:06
Temperature
CO2
Safety controller temperature
Close
Measurement chart view (sample view).
Door temperature
Settings
Home
08.03.2013 05:05:06
Press the “Settings” button.
Submenu “Settings”. Select the desired function
and press the operating button.
Close Home

13.1 Setting the sampling rate

To define the sampling rate, go to Menu > Measurement chart > Settings > Sampling rate
08.03.2013 05:05:06
Submenu “Sampling rate”. Select the desired interval
and press the operating button.
Close Home
CB (E6) 09/2013 Page 88/144
Fixed value
Display range
Temperature
Humidity
CO2
O2
Safety controller temperature
Door temperature
2
0.00
[°C]
4
5.00
[°C]

13.2 Defining the display range

To define the display range, go to Menu > Measurement chart > Settings > Display range
08.03.2013 05:05:06
Submenu “Display range” (unit with O2 control). Select the desired parameter
and press the operating button.
Close Home
Fixed value
..\ Enter minimum value
08.03.2013 05:05:06
Pos1 End Ok 0 1 3 4 5 6 7 8 9 . -
2
Close Ok Home
Fixed value
08.03.2013 05:05:06
..\ Enter maximum value
End Ok 0 1 2 3 5 6 7 8 9 . - Del
4
Close Ok Home
Entry menu for temperature “Enter minimum value”. Enter the desired value with the operating button
and press the operating button to confirm.
Entry menu for temperature “Enter maximum value”. Enter the desired value with the operating button
and press the operating button to confirm.
You can now define the view range of the other parameters or press the “Close” button twice to go back to the graphic display.
CB (E6) 09/2013 Page 89/144
Fixed value
Parameters
Temperature On
Humidity On
CO2 On
O2 On
Safety controller temperature On
Door temperature On

13.3 Selecting the parameters

Here you can select the parameters, which shall be displayed graphically. To select the parameters, go to
Menu > Measurement chart > Settings > Parameters
08.03.2013 05:05:06
Submenu “Parameters” (unit with O2 control). The current setting for each parameter is displayed. Select the desired parameter
and press the operating button to change its status On/Off.
The modified setting is displayed.
Close Home
Press the “Close” button twice to go back to the graphic display. If any parameter was set to “Off”, it will not be included in the graphical display.
CB (E6) 09/2013 Page 90/144

14. Temperature safety devices

14.1 Overtemperature protective device (class 1)

The CO2 incubator is equipped with an internal temperature safety device, class 1 acc. to DIN 12880. It serves to protect the unit and prevents dangerous conditions caused by major defects.
If the actual temperature exceeds the nominal temperature by approx. 10 °C, the over temperature protective device permanently turns off the unit. The user cannot restart the device again. The protective cut-off device is located internally. Only a service specialist can replace it. Therefore, please contact an authorized service provider or BINDER Service.

14.2 Overtemperature safety controller (temperature safety device class 3.1)

The chamber is regularly equipped with an electronic overtemperature safety controller (temperature safety device class 3.1 according to DIN 12880).
The overtemperature safety controller serves to protect the CO
incubator, its environment and the
2
contents from exceeding the maximum permissible temperature. In the case of an error, it limits the temperature inside the chamber to the entered safety controller setpoint.
Please observe the guideline BGI/GUV-I 850-0 on safe working in laboratories (formerly BGR/GUV-R 120 or ZH 1/119 laboratory guidelines issued by the employers’ liability insurance association) (for Germany).
Set the safety controller setpoint by approx. 2 °C above the desired temperature setpoint. Recommended setting: Setpoint type “Offset” with safety controller setpoint 2 °C (factory setting).
The safety controller is functionally and electrically independent of the temperature control system. If an error occurs, it performs a regulatory function.
Check the setting regularly and adjust it following changes of the setpoint or charge.
During a running sterilization (chap. 18.3) the safety controller is non-functional

14.2.1 Safety controller modes

You can select between “Limit (absolute)” and “Offset (relative)” safety controller mode
Limit: Absolute maximum permitted temperature value
Example: This setting offers high safety as a defined temperature limit will not be exceeded. It is important to
adapt the safety controller setpoint after each modification of the temperature setpoint. Otherwise, the limit could be too high to ensure efficient protection, or, in the opposite case, it could prevent the controller from reaching an entered setpoint outside the limit range.
Offset: Maximum overtemperature above any active temperature setpoint. The maximum temperature
changes internally and automatically with every setpoint change. It is important to check the safety controller setpoint and safety controller mode occasionally, as it
does not offer a fix, independent limit temperature value, which would never be exceeded. Example: Desired temperature value 37 °C, desired safety controller value: 39 °C Possible settings:
Temperature setpoint Safety controller mode Safety controller setpoint
Limit (absolute) 39 °C
37 °C
Offset (relative) 2 °C
CB (E6) 09/2013 Page 91/144
Fixed value
..\ Safety controller
Mode
Setpoint
Show settings
Fixed value
..\ Safety controller mode
Limit (absolute)
Fixed value
..\ Safety controller
Mode
Setpoint
Show settings

14.2.2 Setting the safety controller

To display and to change the current safety controller settings in the “safety controller” submenu, go to
Menu > Safety controller
Safety controller mode: selection between Limit (absolute) and Offset (relative)
08.03.2013 05:05:06
Submenu “Safety controller” Select “Mode”
and press the operating button.
Close Home
08.03.2013 05:05:06
Close Home
When changing the safety controller mode, the safety controller setpoint which had been active before in this mode becomes active again.
Entering the safety controller setpoint
08.03.2013 05:05:06
Submenu “Safety controller mode”. The current safety controller mode is displayed:
“Limit (absolute)” or “Offset (relative)” To change the mode, press the operating button. The modified safety controller mode is displayed.
Submenu “Safety controller” Select “Setpoint”
and press the operating button.
Close Home
You can also access this submenu to directly enter the safety controller setpoint via
Quick menu > Safety controller setpoint
CB (E6) 09/2013 Page 92/144
Fixed value
..\ Safety controller overtemperature
3
[°C]
Fixed value
..\ Safety controller
Mode
Setpoints
Show settings
Fixed value
Setpoint
Actual
08.03.2013 05:05:06
End Ok 0 1 2
4 5 6 7 8 9 Del
3
Entry menu “Safety controller overtemperature”. Enter the desired value with the operating button
and press the operating button to confirm. Press the “Ok” button to confirm.
Close Ok Home
Go one level back with the “Close” button or back to the initial view with “Home”.
Overview of the current settings
You can check the current settings of the safety controller:
08.03.2013 05:05:06
Submenu “Safety controller” Select “Show settings”
and press the operating button.
Close Home
The overview display shows the setpoints and actual values of the main temperature controller and the safety controller and indicates the safety controller mode.
08.03.2013 05:05:06
Chamber temperature [°C] Safety controller mode Limit (absolute) Safety controller [°C]
37.0 37.6
39.0 37.6
Overview display with “Limit” mode (example values)
Close
CB (E6) 09/2013 Page 93/144
Home
Fixed value
View
Setpoint
Actual
08.03.2013 05:05:06
Chamber temperature [°C]
37.0 37.6
Safety controller mode Offset (relative) Safety controller [°C]
2.0 37.6
Close
Home
Go back to the initial view with “Home”.
Overview display with “Offset” mode (example values)
CB (E6) 09/2013 Page 94/144
Pin allocation of the RS 422 interface (5) on the
Pin 2: RxD (+)
Pin 7: Ground

15. Options

15.1 Communication software APT-COM™ 3 DataControlSystem (option)

The unit is regularly equipped with an Ethernet interface (6) that can connect the BINDER communication software APT-COM™ 3 DataControlSystem. The MAC Address is indicated below the Ethernet interface. The actual temperature, and gas concentration values are given in adjustable intervals. Up to 30 chambers with RS 422 interface can be cross linked. For further information, refer to the operating manual of the BINDER communication software APT-COM™ 3.

15.2 RS 422 interface (option)

With this option, the chamber is equipped with a serial interface RS 422 (5), that can connect the BINDER communication software APT-COM™ 3 DataControlSystem. The MAC Address is indicated below the Ethernet interface. The actual temperature, and gas concentration values are given in adjustable intervals. For further information, refer to the operating manual of the BINDER communication software APT-COM™ 3.
The connection to a computer is established using the CB interface via an interface converter.
unit rear
Pin 3: TxD (+) Pin 4: RxD (-) Pin 5: TxD (-)
15.3 Silicone access ports 30 mm / 1.18 in, closable from both sides with silicon
plugs (option)
(a)
(b)
(c)
Figure 17: Positions of the optional silicon access ports left (a), rear (b), and right (c)
A warning sticker is located above each access port.
CB (E6) 09/2013 Page 95/144
(G)
When operating a CB with silicon access ports, both silicon plugs must tightly close the access ports. If the plugs are inserted in a not-gastight manner, or if plugs are missing, CO control) may escape into the environment. The CO turn off when the unit door is opened.
control and the O2 control (unit with O2 control) only
2
/ O2 / N2 gas (unit with O2
2
WARNING
High concentration of CO2 (> 4 Vol.-%). Risk of death by suffocation. Danger of poisoning.
Tightly close each access port with two plugs during operation.
Note for units with O2 control:
WARNING
High concentration of O2 (> 21 % O2). Fire and explosion hazard through contact of combustible materials with O Risk of burns and other injuries.
Tightly close each access port with two plugs during operation.
.
2

15.4 Interior socket 230V (option)

The T4.12 controller permits turning on and off the voltage of the interior socket (chap. 6.5).
The interior socket (G) is located at the upper left corner on the rear wall of the inner chamber. It is closed with a waterproof lid. The interior socket is suitable to supply electrical devices inside the incubator with 230V AC voltage.
Figure 18: Position of the interior socket 230 V
(G) Interior socket
The maximum head load must not exceed a capacity of 20 W.
CAUTION
Exceeding the setpoint temperature. Damage to the cultures.
Do NOT exceed the maximum head load of 20 W. Do NOT connect equipment with a nominal capacity > 20 W.
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Figure 19: Interior socket (G) (front view)
Figure 20: Supplied plug (front view)
Pin 2 (L)
Pin 1 (N)
Pin 3 (PE)
Pin 2 (L)
Pin 1 (N) Pin 3 (PE)
Heat emission of electrical devices connected inside the CO2 incubator may modify the temperature range.
The maximum load must not exceed 3 Amp.
WARNING
Overload of contacts. Damage to contacts and connection socket. Electrical hazard. Danger of death.
Do NOT exceed the maximum load of 3 A. Do NOT connect any devices with a nominal current > 3 A.
Turning on and off the voltage of the interior socket is possible at the chamber controller through Menu >
Interior socket (optional), see chap. 6.5.
Turning off the incubator at the main power switch also switches the interior socket voltage-free.
Electrical data socket and plug: IP system of protection 65, 230 V 1N ~ 50-60 Hz
CAUTION
Risk of short circuit. Damage to the unit.
Use delivered plug only (IP protection type 65). If the socket is not used, close it with the waterproof lid.
When inserting a heat load into the inner chamber by introducing electrical devices such as shakers or rollers, the ambient temperature of the incubator must not exceed 25 °C / 77°F. Otherwise, temperature control to 37 °C / 98.6°F cannot be assured. When operating electrical devices inside the incubator, always check the desired incubation temperature before introducing any cells.
CAUTION
Excessive inner temperature > 37 °C / 98.6°F when operating electrical devices inside the incubator.
Damage to the cell cultures.
When operating electrical devices inside the incubator: Ambient temperature # 25 °C /
77°F.
Check the temperature observation before introducing any cells.
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(B)

15.5 Analog outputs for temperature and CO2 (option)

With this option, the CO2 incubator is equipped with analog outputs 4-20 mA for temperature and CO2. These outputs allow transmitting data to external data registration systems or devices.
The connection is realized as a DIN socket (3) on the unit rear. A suitable DIN plug is enclosed.
ANALOG OUTPUT 4-20 mA DC
PIN 1: Temperature + PIN 2: Temperature -
2 2
+
-
PIN 3: CO PIN 4: CO
CO
range: 0 vol.-% up to 20 vol.-%
2
Temperature range: 0 °C / 32°F up to +200 °C / 392°F
Figure 21: Pin configuration of the DIN socket (3) on the unit rear

15.6 Access port for extra-low voltage (option)

The access port (B) (8-pin) for extra-low voltage (ELV) consists of a LEMO socket (which can be covered) and a LEMO connector. It is suitable to connect two electrical devices, one inside and one outside the incubator. You can use it e.g. for devices with a monitoring and control part remaining outside the incubator, whereas its mechanical component like roller or shaker systems is located inside the unit.
Figure 22: Position of the access port for extra-low voltage
Maximum power rating 24V AC/DC 2Amp.
Overload of contacts. Damage to contacts and connection socket. Electrical hazard. Danger of death.
Do NOT exceed the maximum load of 24V AC/DC 2Amp. Do NOT connect any devices with a nominal current > 2 Amp. Do NOT connect any devices with a nominal voltage > 24 V DC.
WARNING
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2
1
7
6
8 3 4 5 3
4
6
7
5 2 1 8 3
4
6
7
5 2 1
8
On unit rear: Exterior Lemo socket (8) on the
rear of the unit
Interior: LEMO socket (B) located at the
upper right corner of the rear
Supplied LEMO connector: on external and internal side
wall of the inner chamber
Figure 23: Pin allocation (front view) of the Lemo sockets and plug
When introducing a heat load into the inner chamber by inserting electrical devices like shakers or rollers, the ambient temperature of the incubator must not exceed 25 °C / 77 °F. Otherwise, temperature control to 37 °C / 98.6°F cannot be assured. When operating electrical devices inside the incubator, always check the desired incubation temperature before introducing in any cells.
CAUTION
Excessive inner temperature > 37 °C / 98.6°F when operating electrical devices inside the incubator.
Damage to the cell cultures.
When operating electrical devices inside the incubator: Ambient temperature 25 °C /
77 °F.
Before introducing any cells, check the correct ambient temperature.
15.7 BINDER Gas Supply Service – External bottle changer for CO2, N2 or O2
(option)
The external bottle changer permits automatic switching to a second gas cylinder as soon as the first cylinder is empty. It can be used for a maximum of two CB units.
Instructions 7001-0196 delivered with the external bottle changer (Art. no. 8012-0408) describe its installation and operation.
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15.8 Stands

15.8.1 Stacking stand (option)

We recommend not stacking CB incubators directly on top of one another in order to avoid transmission of shocks and vibrations from one unit to the other. This could happen e.g. while opening or closing the door, cleaning, charging and discharging the unit. BINDER offers stable, vibration-free stands with castors (2 lockable by brakes) for the safe stacking of two units.
The stacking stand ensures that the set incubation parameters are precisely maintained also during sterilization of the other unit in the same stacking stand (chap. 18.3) by thermal decoupling.
Using the stacking stand offers more advantages: You can pull the lower incubator forward separately (e.g., for access to the rear), and not have to place it directly on the floor (important for sanitary purpose).
The mounting instructions 7001-0194 delivered with the stacking stand describe its installation (Art. No. 9051-0020 for CB 160, Art. No. 9051-0023 for CB 220).

15.8.2 Stacking adapter for direct thermal decoupled stacking (option)

We recommend not stacking CB incubators directly on top of one another in order to avoid transmission of shocks and vibrations from one unit to the other. This could happen e.g. while opening or closing the door, cleaning, loading and unloading the unit. BINDER offers a stacking adapter for direct thermal decoupled stacking of two CB incubators.
The stacking adapter ensures the exact maintenance of the set incubation parameters also during sterilization of the other unit in the same stacking stand (chap. 18.3).
The mounting instructions 7001-0145 delivered with the stacking adapter describe its installation (Art. No. 9051-0032 for CB 60, Art. No. 9051-0026 for CB 160, Art. No. 9051­0030 for CB 220).

15.8.3 Base on castors (option)

In order to obtain easy access to the incubator and to avoid contamination of the incubator caused by soil pollution, BINDER recommends using the base on castors.
The mounting instructions 7001-0147 delivered with the base on castors describe its installation (Art. No. 9051-0031 for CB 60, Art. No. 9051-0028 for CB 160, Art. No. 9051­0029 for CB 220).
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