BINDER CB 60 User Manual

Operating Manual

APT.line™ CB (E6)
CO
CO

- Incubators

2

– Incubators with O2 control

2

with sterilizable NDIR sensor system for CO2 and humidity
and microprocessor controller T4.12

Model Voltage Equipment Art. No.

CB 60 230 V 9040-0088, 9140-0088

CB 60-UL 100-120V 9040-0089, 9140-0089

CB 60 230 V with O2 control 9040-0090, 9140-0090

CB 60-UL 100-120V with O2 control 9040-0091, 9140-0091

CB 160 (E6) 230 V 9040-0092, 9140-0092

CB 160-UL 100-120V 9040-0093, 9140-0093

CB 160 (E6) 230 V with O2 control 9040-0094, 9140-0094

CB 160-UL 100-120V with O2 control 9040-0095, 9140-0095

CB 160 230 V with divided glass door 9040-0100, 9140-0100

CB 160-UL 100-120V with divided glass door 9040-0101, 9140-0101

CB 160 230 V with O2 control with divided glass door 9040-0102, 9140-0102

CB 160-UL 100-120V with O2 control with divided glass door 9040-0103, 9140-0103

CB 220 230 V 9040-0096, 9140-0096

CB 220-UL 100-120V 9040-0097, 9140-0097

CB 220 230 V with O2 control 9040-0098, 9140-0098

CB 220-UL 100-120V with O2 control 9040-0099, 9140-0099

BINDER GmbH

Address Post office box 102
D-78502 Tuttlingen
Tel. [phone]
Fax [phone]
Internet http://www.binder-world.com
E-mail [e-mail]
Service Hotline [phone]
Service Fax [phone]
Service E-Mail [e-mail]
Service Hotline USA [phone] or [phone] x3
Service Hotline Asia Pacific [phone] or [phone]
Service Hotline Russia and CIS [phone]

Issue 09/2013 Art. No. 7001-0229

EG – KONFORMITÄTSERKLÄRUNG

Anschrift / Address / Adresse:

Typenbezeichnung / Type / Type:

employé dans certaines limites de tension

EG – declaration of conformity

EC - DECLARATION OF CONFORMITY
CE - DECLARATION DE CONFORMITE

Anbieter / Supplier / Fournisseur:

Produkt / Product / Produit:

Die oben beschriebenen Produkte sind konform mit folgenden EG-Richtlinien:
The products described above are in conformity with the following EC guidelines:
Les produits décrits ci-dessus sont conformes aux directives CE suivantes:

Niederspannungsrichtlinie
2006/95/EG

Low voltage directive
2006/95/EC

Directive basse tension
2006/95/CE

BINDER GmbH
Im Mittleren Ösch 5, D-78532 Tuttlingen
CO

Begasungsbrutschrank

2

Incubators

CO

2

Incubateurs à CO
CB 60, CB 160, CB 220

Richtlinie 2006/95/EG des Europäischen Parlaments und des
Rates vom 12. Dezember 2006 zur Angleichung der
Rechtsvorschriften der Mitgliedstaaten betreffend elektrische
Betriebsmittel zur Verwendung innerhalb bestimmter
Spannungsgrenzen

Council Directive 2006/95/EC of 12 December 2006 on the
harmonization of the laws of Member States relating to electrical
equipment designed for use within certain voltage limits

Directive 2006/95/CE du Parlement Européen et du Conseil du 12
décembre 2006 concernant le rapprochement des législations des
États membres relatives au matériel électrique destiné à être

2

EMV-Richtlinie
2004/108/EG

EMC Directive
2004/108/EC

Directive CEM
2004/108/CE

Die oben beschriebenen Produkte tragen entsprechend die Kennzeichnung CE.
The products described above, corresponding to this, bear the CE-mark.
Les produits décrits ci-dessus, en correspondance, portent l’indication CE.

CB (E6) 09/2013 Page 2/144

Richtlinie 2004/108/EG des Europäischen Parlaments und des
Rates vom 15. Dezember 2004 zur Angleichung der
Rechtsvorschriften der Mitgliedstaaten über die
elektromagnetische Verträglichkeit und zur Aufhebung der
Richtlinie 89/336/EWG.

Directive 2004/108/EC of the European Parliament and of the
Council of 15 December 2004 on the approximation of the laws of
the Member States relating to electromagnetic compatibility and
repealing Directive 98/336/EEC.

Directive 2004/108/CE du Parlement Européen et du Conseil du
15 décembre 2004 relative au rapprochement des législations des
États membres concernant la compatibilité électromagnétique et
abrogeant le directive 98/336/CEE.

1 / 3

Sicherheit / safety / sécurité:

EMV / EMC / CEM:

Die oben beschriebenen Produkte sind konform mit folgenden harmonisierten Normen:
The products described above are in conformity with the following harmonized standards:
Les produits décrits ci-dessus sont conformes aux normes harmonisées suivantes:

EN 61010-1:2010 Sicherheitsbestimmungen für elektrische Mess-, Steuer-, Regel- und

Laborgeräte – Teil 1: Allgemeine Anforderungen (DIN EN 61010­1:2011, VDE 411-1:2011)

Safety requirements for electrical equipment for measurement, control,
and laboratory use – Part 1: General requirements (IEC 61010-1:2010,
BS EN 61010-1:2010)

Règles de sécurité pour appareils électriques de mesurage, de
régulation et de laboratoire – Partie 1: Prescriptions générales (CEI
61010-1:2010, NF EN 61010:2011)

EN 61010-2-010:2003 Sicherheitsbestimmungen für elektrische Meß-, Steuer-, Regel- und

Laborgeräte – Teil 2-010: Besondere Anforderungen an Laborgeräte für
das Erhitzen von Stoffen (DIN EN 61010-2-010:2004)

Safety requirements for electrical equipment for measurement, control,
and laboratory use – Part 2-010: Particular requirements for laboratory
equipment for the heating of materials (IEC 61010-2-10:2005, BS EN
61010-2-10:2003)

Règles de sécurité pour appareils électriques de mesurage, de
régulation et de laboratoire – Partie 2-010 : Prescriptions particulières
pour appareils de laboratoire utilisés pour l’échauffement des matières
(CEI 61010-2-10:2003, NF EN 61010-2-10:2005)

EN 61326-1:2006
+ Corr. 1:2008 + Corr. 2:2010

Elektrische Mess-, Steuer-, Regel- und Laborgeräte - EMV­Anforderungen - Teil 1: Allgemeine Anforderungen (DIN EN 61326­1:2006 + Berichtigung 1:2008 + Berichtigung 2:2011)

Electrical equipment for measurement, control and laboratory use ­EMC requirements - Part 1: General requirements (IEC 61326-1:2005 +
Corr. 1:2008 + Corr. 2:2010, BS EN 61326-1:2006+ A1:2008)

Matériel électrique de mesure, de commande et de laboratoire ­Exigences relatives à la CEM - Partie 1: Exigences générales (CEI
61326-1:2005 + AC1:2008, NF EN 61326-1:2006 mod.)

EN 61326-2-2:2006 Elektrische Mess-, Steuer-, Regel- und Laborgeräte – EMV-

Anforderungen. Teil 2-2: Besondere Anforderungen - Prüfanordnung,
Betriebsbedingungen und Leistungsmerkmale für ortsveränderliche
Prüf-, Mess- und Überwachungsgeräte in Niederspannungs­Stromversorgungsnetzen. (DIN EN 61326-2-2:2006)

Electrical equipment for measurement, control and laboratory use –
EMC requirements. Part 2-2: Particular requirements - Test
configurations, operational conditions and performance criteria for
portable test, measuring and monitoring equipment used in low-voltage
distribution systems. (IEC 61326-2-2:2005, BS EN 61326-2-2:2006)

Matériel électrique de mesure, de commande et de laboratoire –
Exigences relatives à la CEM. Partie 2-2: Exigences particulières ­Configurations d’essai, conditions de fonctionnement et critères
d’aptitude à la fonction des matériels portatifs d’essai, de mesure et de
surveillance utilisés dans des systèmes de distribution basse tension.
(CEI 61326-2-2:2005 + AC1:2007, NF EN 61326-2-2:2006)

CB (E6) 09/2013 Page 3/144

2 / 3

P. M. Binder

B. Hofmann

D-78532 Tuttlingen, 20.07.2012
BINDER GmbH

Geschäftsführender Gesellschafter
Managing Director
Directeur général

Leiter F & E
Director R & D
Chef de service R&D

3 / 3

CB (E6) 09/2013 Page 4/144

Product registration

CB (E6) 09/2013 Page 5/144

Contents

EG – declaration of conformity ...................................................................................................................... 2

Product registration ....................................................................................................................................... 5

1. SAFETY ................................................................................................................ 10

1.1 Legal considerations ......................................................................................................................... 10

1.2 Structure of the safety instructions .................................................................................................... 10

1.2.1 Signal word panel ................................................................................................................... 10

1.2.2 Safety alert symbol ................................................................................................................. 11

1.2.3 Pictograms .............................................................................................................................. 11

1.2.4 Word message panel structure ............................................................................................... 12

1.3 Localization / position of safety labels on the unit ............................................................................. 12

1.4 Type plate.......................................................................................................................................... 14

1.5 General safety instructions on installing and operating the CO2 incubator....................................... 15

1.6 Precautions when working with gases .............................................................................................. 16

1.7 Precautions when handling gas cylinders ......................................................................................... 18

1.8 Intended use ..................................................................................................................................... 18

2. UNIT DESCRIPTION ............................................................................................ 19

2.1 Unit overview ..................................................................................................................................... 20

2.2 Instrument panel ............................................................................................................................... 20

2.3 Inner chamber ................................................................................................................................... 21

2.4 Control panel on the rear of the unit ................................................................................................. 23

3. COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND

INSTALLATION .................................................................................................... 24

3.1 Unpacking, and checking equipment and completeness of delivery ................................................ 24

3.2 Guidelines for safe lifting and transportation ..................................................................................... 24

3.3 Storage .............................................................................................................................................. 25

3.4 Location of installation and ambient conditions ................................................................................ 25

4. INSTALLATION AND CONNECTIONS ............................................................... 29

4.1 Shelves .............................................................................................................................................. 29

4.2 Permadry™ water pan ...................................................................................................................... 29

4.3 Gas connections ............................................................................................................................... 30

4.3.1 Connection of the CO2 gas cylinder ........................................................................................ 31

4.3.2 Connection of the O2 gas cylinder (unit with O2 control) ......................................................... 32

4.3.3 Connection of the N2 gas cylinder (unit with O2 control) ......................................................... 33

4.3.4 Connecting the gas hose to the unit rear (for CO2, O2, N2) .................................................... 34

4.3.5 Gas cylinder connection kits (option) ...................................................................................... 35

4.4 Electrical connection ......................................................................................................................... 36

4.5 Handling and aligning the multiple-divided inner glass door, gas proof (optional equipment) ........ 37

5. START UP ............................................................................................................ 38

5.1 Equilibration time ............................................................................................................................... 38

5.2 Factory settings ................................................................................................................................. 38

6. FUNCTIONAL OVERVIEW OF THE T4.12 CHAMBER CONTROLLER ............. 39

6.1 Menu structure .................................................................................................................................. 40

6.1.1 General menu ......................................................................................................................... 40

6.1.2 Quick menu ............................................................................................................................. 41

6.1.3 User menu .............................................................................................................................. 42

6.2 Operating modes ............................................................................................................................... 43

6.2.1 Activating the “control off” mode or change to “fixed value” operating mode ......................... 43

CB (E6) 09/2013 Page 6/144

Deactivating the O

6.3

6.3.1 Required gas supply of the unit with O2 control ...................................................................... 45

6.3.2 Activating / deactivating the O2 control and O2 / N2 pressure alarms ..................................... 46

6.4 Humidity control of the Permadry™ system ...................................................................................... 47

6.5 Turning on / off the interior socket voltage (with optional interior socket) ......................................... 48

6.6 Performance during and after power failure ...................................................................................... 48

6.7 Information ........................................................................................................................................ 49

control and O2/N2 pressure alarms (unit with O2 control) .................................. 45

2

7. SETPOINT ENTRY ............................................................................................... 50

7.1 Setting ranges ................................................................................................................................... 50

7.2 Note when setting high gas concentrations ...................................................................................... 50

7.3 Entering the setpoints via “quick menu” ............................................................................................ 52

7.4 Entering the setpoints via general menu ........................................................................................... 54

8. KEY LOCK ........................................................................................................... 56

8.1 Directly activating the key lock .......................................................................................................... 57

8.2 Automatic key lock ............................................................................................................................ 57

8.3 Changing the password for unlocking the key lock ........................................................................... 59

9. GENERAL CONTROLLER SETTINGS ................................................................ 60

9.1 Setup wizard ..................................................................................................................................... 61

9.2 Date and time settings ...................................................................................................................... 61

9.3 Selecting the menu language of the T4.12 controller ....................................................................... 63

9.4 Setting display brightness ................................................................................................................. 63

9.5 Changing the temperature unit.......................................................................................................... 64

9.6 Defining the data recording rate ........................................................................................................ 64

9.7 Factory reset ..................................................................................................................................... 65

9.8 Network configuration ....................................................................................................................... 65

9.9 Display of the network configuration ................................................................................................. 69

9.10 MAC Address .................................................................................................................................... 69

9.11 RS 422 address (with optional RS 422 interface) ............................................................................. 70

9.12 Display and entry of the device configuration – for service purpose ................................................ 71

10. DATA TRANSFER VIA USB INTERFACE........................................................... 72

10.1 Exporting data to USB drive .............................................................................................................. 72

10.2 Importing data from USB drive .......................................................................................................... 73

11. NOTIFICATIONS AND ALARMS ......................................................................... 74

11.1 Notifications overview ....................................................................................................................... 74

11.2 Alarm messages overview ................................................................................................................ 74

11.3 Alarm status ...................................................................................................................................... 76

11.4 Confirming an active “set” alarm ....................................................................................................... 77

11.5 Alarm configuration and overview ..................................................................................................... 78

11.5.1 List of active alarms ................................................................................................................ 78

11.5.2 History – list of all alarms ........................................................................................................ 79

11.5.3 Activating, deactivating, and testing the alarm buzzer ........................................................... 80

11.5.4 Activating / deactivating all alarm functions ............................................................................ 80

11.5.5 Setting the delay time after opening the door ......................................................................... 81

11.5.6 Tolerance ranges and alarm delay times................................................................................ 81

11.6 Zero-voltage relay alarm output ........................................................................................................ 86

12. EVENT LIST ......................................................................................................... 87

13. GRAPHICAL DISPLAY OF THE MEASURED VALUES ..................................... 88

13.1 Setting the sampling rate .................................................................................................................. 88

13.2 Defining the display range................................................................................................................. 89

13.3 Selecting the parameters .................................................................................................................. 90

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14. TEMPERATURE SAFETY DEVICES ................................................................... 91

14.1 Overtemperature protective device (class 1) .................................................................................... 91

14.2 Overtemperature safety controller (temperature safety device class 3.1) ........................................ 91

14.2.1 Safety controller modes .......................................................................................................... 91

14.2.2 Setting the safety controller .................................................................................................... 92

15. OPTIONS .............................................................................................................. 95

15.1 Communication software APT-COM™ 3 DataControlSystem (option) ............................................ 95

15.2 RS 422 interface (option) .................................................................................................................. 95

15.3 Silicone access ports 30 mm / 1.18 in, closable from both sides with silicon plugs (option) ............ 95

15.4 Interior socket 230V (option) ............................................................................................................. 96

15.5 Analog outputs for temperature and CO2 (option) ............................................................................ 98

15.6 Access port for extra-low voltage (option) ......................................................................................... 98

15.7 BINDER Gas Supply Service – External bottle changer for CO2, N2 or O2 (option) ......................... 99

15.8 Stands ............................................................................................................................................. 100

15.8.1 Stacking stand (option) ......................................................................................................... 100

15.8.2 Stacking adapter for direct thermal decoupled stacking (option) ......................................... 100

15.8.3 Base on castors (option) ....................................................................................................... 100

16. REFERENCE MEASUREMENTS ...................................................................... 101

16.1 CO2 reference measuring ............................................................................................................... 101

16.1.1 Measuring CO2 concentration indirectly via the pH of the cell medium ................................ 101

16.1.2 Measuring CO2 directly via chemical indicator tubes............................................................ 102

16.1.3 Measuring CO2 directly with an electronic infrared measuring device ................................. 103

16.2 Temperature reference measurement ............................................................................................ 103

17. AVOIDING MICROBIAL CONTAMINATION...................................................... 103

17.1 Cells and media .............................................................................................................................. 103

17.2 Laboratory conditions / equipment around the incubator ................................................................ 103

17.3 Working and behavior in the lab ..................................................................................................... 104

17.4 Chamber design and equipment of the CO2 incubator ................................................................... 104

17.5 Handling the CO2 incubator ............................................................................................................ 105

18. CLEANING, DECONTAMINATION / DISINFECTION, AND STERILIZATION .. 107

18.1 Cleaning .......................................................................................................................................... 107

18.2 Decontamination / chemical disinfection of the CO2 incubator ....................................................... 108

18.3 Hot-air sterilization at 180 °C / 356 °F ............................................................................................. 110

18.3.1 Overview ............................................................................................................................... 110

18.3.2 Performing a hot-air sterilization ........................................................................................... 111

18.3.3 Aborting the hot-air sterilization prematurely ........................................................................ 113

18.3.4 End of the sterilization cycle ................................................................................................. 115

19. MAINTENANCE AND SERVICE ........................................................................ 116

19.1 Maintenance intervals, service ........................................................................................................ 116

19.2 Checking the air jacket heating fan ................................................................................................. 116

19.3 Checking the humidity system fan .................................................................................................. 117

19.4 Gas inlet fine filter ........................................................................................................................... 117

19.5 Sending the unit back to BINDER GmbH ....................................................................................... 117

20. DISPOSAL.......................................................................................................... 118

20.1 Disposal of the transport packing .................................................................................................... 118

20.1.1 Outer unit packing ................................................................................................................. 118

1.1.1 Packing inside the unit and equipment ................................................................................. 118

20.2 Decommissioning ............................................................................................................................ 119

20.3 Disposal of the unit in the Federal Republic of Germany ............................................................... 119

20.4 Disposal of the unit in the member states of the EC except for the Federal Republic of Germany 120

20.5 Disposal of the unit in non-member states of the EC ..................................................................... 121

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21. TROUBLESHOOTING ....................................................................................... 122

21.1 Heating ............................................................................................................................................ 122

21.2 Gas cylinder pressure too low ......................................................................................................... 123

21.3 Gas concentration ........................................................................................................................... 125

21.4 Sterilization ...................................................................................................................................... 127

21.5 Humidity .......................................................................................................................................... 127

21.6 Controller ......................................................................................................................................... 129

21.7 Open door ....................................................................................................................................... 129

22. TECHNICAL DESCRIPTION .............................................................................. 130

22.1 Factory calibration and adjustment ................................................................................................. 130

22.2 Over current protection ................................................................................................................... 130

22.3 Definition of usable volume ............................................................................................................. 130

22.4 CB technical data ............................................................................................................................ 131

22.5 Equipment and Options ................................................................................................................... 133

22.6 Spare parts and accessories .......................................................................................................... 134

22.7 Important conversion data for non-SI units ..................................................................................... 135

22.8 Conversion table for gas inlet pressures, bar – psi ......................................................................... 135

22.9 Dimensions CB 60 .......................................................................................................................... 136

22.10 Dimensions CB 160 ........................................................................................................................ 137

22.11 Dimensions CB 220 ........................................................................................................................ 138

23. CONTAMINATION CLEARANCE CERTIFICATE ............................................. 139

23.1 For units located outside North America and Central America ....................................................... 139

23.2 For units in North America and Central America ............................................................................ 142

CB (E6) 09/2013 Page 9/144

Dear customer,

For the correct operation of the CO
completely and carefully and observe all instructions as indicated. Failure to read, understand and follow
the instructions may result in personal injury. It can also lead to damage to the unit and/or poor equipment
performance

incubator CB, it is important that you read this operating manual

2

1. Safety

This operating manual is part of the components of delivery. Always keep it handy for reference. The
device should only be operated by laboratory personnel especially trained for this purpose and familiar
with all precautionary measures required for working in a laboratory. To avoid injuries and damage
observe the safety instructions of the operating manual.

WARNING

Failure to observe the safety instructions.
Serious injuries and unit damage.

 Observe the safety instructions in this operating manual.
 Carefully read the complete operating instructions of the CO

1.1 Legal considerations

This operating manual is for informational purposes only. It contains information for installing, start-up,
operation and maintenance of the product. Note: the contents and the product described are subject to
change without notice.

incubator CB.

2

Understanding and observing the instructions in this operating manual are prerequisites for hazard-free
use and safety during operation and maintenance. In no event shall BINDER be held liable for any
damages, direct or incidental arising out of or related to the use of this manual.

This operating manual cannot cover all conceivable applications. If you would like additional information,
or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or
contact us directly by phone at the number located on page one of this manual

Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or
existing agreement, description, or legal relationship, nor do they modify such a relationship. All
obligations on the part of BINDER derive from the respective purchase contract, which also contains the
entire and exclusively valid statement of warranty administration. The statements in this manual neither
augment nor restrict the contractual warranty provisions.

1.2 Structure of the safety instructions

In this operating manual, the following safety definitions and symbols indicate dangerous situations
following the harmonization of ISO 3864-2 and ANSI Z535.6.

1.2.1 Signal word panel

Depending on the probability of serious consequences, potential dangers are identified with a signal
word, the corresponding safety color, and if appropriate, the safety alert symbol.

DANGER

Indicates an imminently hazardous situation that, if not avoided, will result in death or serious
(irreversible) injury.

CB (E6) 09/2013 Page 10/144

Warning signs

Electrical hazard

Hot surface

Explosive atmosphere

poisoning hazard

or chemical burns

Biohazard

WARNING

Indicates a potentially hazardous situation which, if not avoided, could result in death or serious
(irreversible) injury.

CAUTION

Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor
(reversible) injury.

CAUTION

Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product
and/or its functions or of a property in its proximity.

1.2.2 Safety alert symbol

Use of the safety alert symbol indicates a risk of injury.
Observe all measures that are marked with the safety alert symbol in order to avoid death or

injury.

1.2.3 Pictograms

Lifting hazard

Explosive substances

Gas cylinders

Fire promoting agents

Pollution Hazard

Suffocation hazard

Harmful substances

Stability hazard

CO2 suffocation and

Risk of corrosion and /

CB (E6) 09/2013 Page 11/144

Mandatory action signs

plug

Environment protection

Wear protective gloves

Prohibition signs

water

Pictograms (Warning signs)

Service label

Mandatory regulation

Do NOT touch

Information to be observed in order to ensure optimum function of the product.

Read operating

instructions

Do NOT spray with

1.2.4 Word message panel structure

Type / cause of hazard.
Possible consequences.

 Instruction how to avoid the hazard: prohibition
 Instruction how to avoid the hazard: mandatory action

Disconnect the power

Wear safety goggles

Do NOT climb

Lift with several persons

Observe all other notes and information not necessarily emphasized in the same way, in order to avoid
disruptions that could result in direct or indirect injury or property damage.

1.3 Localization / position of safety labels on the unit

The following labels are located on the unit:

Hot surface

Risk of injury

• on the outer door: CB-UL only

• above the access ports (option)

CB (E6) 09/2013 Page 12/144

CO2 -Incubator CB CO2 -Incubator CB-UL

Figure 1: Position of labels on the unit

Keep safety labels complete and legible.

Replace safety labels that are no longer legible. Contact BINDER service for these replacements.

CB (E6) 09/2013 Page 13/144

Indications of the type plate

Information

BINDER

Manufacturer: BINDER GmbH

CB 160

Model CB 160

Serial No.

00-00000

Serial No. 00-00000

Nominal temperature

187 °C
369 °F

Enclosure protection

IP 20

IP type of protection 20 acc. to EN 60529

Temp. safety device

DIN 12880

Temperature safety device acc. to standard DIN 12880

Class

3.1

Temperature safety device, class 3.1

Art. No.

9040-0092

Art. No. 9040-0092

Project No.

---

(Special application acc. to project no.)

1,30 kW

Nominal power 1,30 kW

200-240 V 1 N ~

Nominal voltage 200-240 V ± 10%, single-phase unit

5,7 A

Nominal current 5,7 Amp

50/60 Hz

Power frequency 50/60 Hz

Symbol on the type plate

Information

LABORATORY EQUIPMENT
43KM

Nominal temperature

187 °C

1,30 kW

369°F

200-240 V 1 N ~

Temp. safety device

IP 20
DIN 12880

50/60 Hz

Class

3.1

Art. No.

9040-0092

US PATS 4585923 / 5222612 / 5309981

Project No.

5405194 / 5601143 / 5773287 / 6079403

D 78532 Tuttlingen / Germany

Internet: www.binder-world.com

1.4 Type plate

Position of type plate: left unit side (seen from front), at the bottom in the middle

Enclosure protection

5,7 A

Tel. + 49 (0) 7462/ 2005-0

CB 160 Serial No. 00-00000

Figure 2: Type plate (example of CB 160 regular unit)

Nominal temperature

Made in Germany

CE conformity marking

Electrical and electronic equipment manufactured / placed on
the market in the EC after 13 August 2005 and to be disposed of
in separate collection according to directive 2002/96/EC on
waste electrical and electronic equipment (WEEE).

The equipment is certified in the GOST R certification system of
GOSTSTANDARD Russia.

The equipment is certified by Underwriters Laboratories Inc.®
according to standards CAN/CSA-C22.2 No. 61010-1, 2

nd

Edition, 2004-07 (Electrical Equipment for Measurement,

(CB-UL only)

Control, and Laboratory Use; Part 1: General Requirements); UL
61010-1, 2
Measurement, Control, and Laboratory Use; Part 1: General
Requirements); IEC 61010-1:2010, 3

nd

Edition, 2005-07-22 (Electrical Equipment for

rd

Edition and IEC 61010-2­10:2003 (Particular Requirements for Laboratory Equipment for
the heating of materials).

CB (E6) 09/2013 Page 14/144

1.5 General safety instructions on installing and operating the CO2 incubator

With regard to operating the CO2 incubator CB and to the installation location, please observe the
guideline BGI/GUV-I 850-0 on safe working in laboratories (formerly BGR/GUV-R 120 or ZH 1/119
laboratory guidelines issued by the employers’ liability insurance association) (for Germany).

BINDER GmbH is only responsible for the safety features of the unit provided skilled electricians or
qualified personnel authorized by BINDER perform all maintenance and repair, and if components
relating to chamber safety are replaced in the event of failure with original spare parts.

To operate the unit, use only original BINDER accessories or accessories from third-party suppliers
authorized by BINDER. The user is responsible for any risk caused by using unauthorized accessories.

CAUTION

Danger of overheating.
Damage to the unit.

∅ Do NOT install the unit in unventilated recesses.

 Ensure sufficient ventilation for dispersal of the heat.

Do not operate the CO2 incubator CB in hazardous locations.

DANGER

Explosion hazard.
Danger of death.

∅ Do NOT operate the unit in potentially explosive areas.

 KEEP explosive dust or air-solvent mixtures AWAY from the unit.

The CO2 incubator CB does not dispose of any measures of explosion protection.

DANGER

Explosion hazard.
Danger of death.

∅ Do NOT introduce any substance into the CO2 incubator which is combustible or

explosive at working temperature.

∅ NO explosive dust or air-solvent mixture in the inner chamber.

Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of
the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature
inside the chamber must lie below the flash point or below the sublimation point of the charging material.
Familiarize yourself with the physical and chemical properties of the charging material, as well as the
contained moisture constituent and its behavior with the addition of heat energy.

Familiarize yourself with any potential health risks caused by the charging material, the contained
moisture constituent or by reaction products which may arise during the temperature process. Take
adequate measures to exclude such risks prior to putting the CO

incubator into operation.

2

DANGER

Electrical hazard.
Danger of death.

∅ The unit must NOT become wet during operation or maintenance.

The CO2 incubators were produced in accordance with VDE regulations and were routinely tested in
accordance to VDE 0411-1 (IEC 61010-1).

CB (E6) 09/2013 Page 15/144

CAUTION

The glass doors and the inner chamber will become hot during operation.
Danger of burning.

∅ Do NOT touch the glass doors, the inner surfaces or the charging material during

operation.

WARNING

Stability hazard.
Danger of injury.
Damage to the unit and the charging material.
Housing cover breakaway.

∅ Do NOT climb on the lower housing cover.
∅ Do NOT load the lower housing cover with heavy objects while the unit door is open.

1.6 Precautions when working with gases

Notes on handling carbon dioxide (CO2)

Carbon dioxide (CO
and therefore practically imperceptible. Vent out any CO
or a suitable connection to an exhaust system. We recommend installing a CO

) in high concentrations is hazardous to health. It is colorless and almost odorless

2

gas that may escape via good room ventilation

2

WARNING

High concentration of CO2 (> 4 Vol.-%).
Risk of death by suffocation.
Danger of poisoning.

∅ Do NOT set up units in non-ventilated recesses.

 Ensure technical ventilation measures.
 Observe the relevant regulations for handling CO

.

2

warning system

2

CB (E6) 09/2013 Page 16/144

t and necessary safety

ition sources in areas where oxygen enrichment is

Unit with O

Oxygen (O

control: Notes on handling oxygen (O2)

2

) is colorless and almost odorless and therefore practically imperceptible. It promotes burns,

2

which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes
and hair. O

is heavier than air and may accumulate in low-lying areas.

2

WARNING

High concentration of O2 (> 21 % O2).
Fire and explosion hazard through contact of combustible materials with O
Risk of burns and other injuries.

∅ Do NOT set up units in non-ventilated recesses.

 Ensure technical ventilation measures.
 Observe the relevant regulations for handling O

.

2

Take appropriate measures to prevent oxygen enrichment and fire and explosion hazards in areas where
oxygen enrichment is possible.

General information for safe handling of oxygen:

• Make sure training of personnel on hazards of oxygen enrichmen
measures.

• Make sure adequate labeling of all oxygen equipment and facilities.

• Make sure gas tightness of all gas connections by checking them for leaks (e.g. with leak

spray or diluted soap solution).

.

2

• Close the main valve of the source of oxygen after work when not using the chamber.

• Never lubricate O

equipment with oil or fat. Use only materials and spare parts which are

2

approved for use with oxygen.

• Regularly inspect fire extinguishers for proper condition.

• Set up emergency showers where oxygen enrichment is possible.

• Strictest smoking ban and no ign

possible.

• Make sure good ventilation of areas where oxygen enrichment is possible (location of the
chamber and/or O

cylinders.

2

• Persons who may have been in a possibly oxygen-enriched atmosphere must keep away
from ignition sources (flames, cigarettes, etc.) and ventilate their clothes at least 15
minutes.

• Always keep emergency routes free.

Unit with O2 control: Notes on handling nitrogen (N2)

Nitrogen (N
therefore practically imperceptible. Any N

) in high concentrations is hazardous to health. It is colorless and almost odorless and

2

gas that may escape must be safely led out via good room

2

ventilation or a suitable connection to an exhaust system.

WARNING

High concentration of N2.
Risk of death by suffocation.

∅ Do NOT set up units in non-ventilated recesses.

 Ensure technical ventilation measures.
 Observe the relevant regulations for handling N

.

2

CB (E6) 09/2013 Page 17/144

1.7 Precautions when handling gas cylinders

General information for safe handling of gas cylinders:

• Store and use gas cylinders only in well-ventilated locations.

• Open the gas cylinder valve slowly to avoid pressure surges.

• Secure gas cylinders during storage and use against falling (chaining).

• Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them.

• Always close the valve even with apparently empty cylinders; screw on the cap when not in

use. Return gas cylinders with the valve closed.

• Do not open gas cylinders by force. Mark them when damaged.

• Protect gas cylinders against fire, e.g. do not store together with flammable liquids.

• Observe relevant regulations for dealing with gas cylinders.

Secure the gas cylinders against falling and other mechanical damage.

WARNING

Safety valve tearing off.
Sudden release of the stored pressure energy.
Risk of injury.

 Secure gas cylinders against falling (chaining).
 Transport gas cylinders with a cylinder cart.

The valve of the gas cylinder always must be closed before screwing on or unscrewing the gas hose.

WARNING

Opening the cylinder valve when the cylinder is not connected.
Sudden release of the stored pressure energy.
Risk of injury.

 Close the gas cylinder valve before connecting or removing the gas hose.

After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or
diluted soap solution).

1.8 Intended use

Series CB incubators are suitable for the cultivation of mammal cells under typical conditions of approx.
37 °C / 98.6°F. The incubator permits setting defined pH conditions by common NaHCO
of commercial cell media by keeping an exact CO
humidity inside to avoid osmolarity increasing caused by the evaporation of the cell media.

With the unit with O
cells.

Following the instructions in this operating manual and conducting regular maintenance work
(chap. 19.1) are part of the intended use.

control, a variable oxygen atmosphere can additionally influence the growth of the

2

atmosphere inside. CB incubators guarantee high

2

buffer systems

3

Other applications are not approved.

WARNING: If customer should use a BINDER chamber running in non-supervised
continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen
or samples to split such specimen or samples and store them in at least two chambers, if this
is feasible.

CB (E6) 09/2013 Page 18/144

2. Unit description

The CO2 incubators CB are equipped with a multifunctional microprocessor display controller for
temperature, CO
degree resp. 0.1 vol.-%

The inner chamber, the pre-heating chamber and the inside of the doors are all made of stainless steel
(material no. 1.4301 (V2A) in Germany). The inner surfaces are smooth and therefore easy to clean. The
inner chamber is deep-drawn from one piece, polished (suitable for pharmaceutical applications) and has
no welds or inaccessible corners. The hinges and the seal of the inner glass door are glued from the
outside to aid cleaning of the inner chamber. When operating the chamber at high temperatures
(sterilization), the impact of the oxygen in the air may cause discoloration of the metallic surfaces
(yellowish-brown or blue) by natural oxidation processes. These colorations are harmless and will in no
way impair the function or quality of the unit.

The perforated shelves are also made of stainless steel. You can insert a maximum of 3 (CB 60), 6 (CB

160), resp. 8 (CB 220) shelves.
The housing is RAL 7035 powder-coated. All corners and edges are also completely coated.

, and O2 (unit with O2 control) levels and a digital display accurate to one-tenth of a

2

The heating system of the CO

incubator permits hot-air auto-sterilization at a setpoint of 187.5 °C /

2

369.5°F. Thus, a temperature of 180 °C / 356°F is maintained for at least 30 minutes on all internal
surfaces, resulting in sterilization of the entire inner chamber. Therefore, this procedure meets all
international guidelines regarding hot air sterilization, e.g. AAMI ST63, DIN 58947, European
Pharmacopoeia.

Thanks to the standard safety device (class 3.1 according to DIN 12880), the set temperature is
maintained in case of failure.

The gas enters the chamber via a fine filter (aseptic filter) with a high filtration efficiency that also filters
the smallest particles.

A highly precise, drift-free CO

gas through a special proprietary gas mixing head developed by BINDER allows precise and

CO

2

constant CO
The CO

and O2 sensors

CO

2

concentrations for long periods. This creates optimum growth conditions for cultures.

2

incubator is also available with O2 control in addition to CO2 control.

2

Fast reaction times, maximum accuracy and selectivity characterize the CO
the CB incubator series. The accuracy of the CO

infrared measuring system in combination with the permanent mixture of

2

measuring procedure of

measuring system is based on a double-beam

2

2

infrared measuring cell with NDIR (non-dispersive infrared) sensor, which continuously regulates to a
reference value. Therefore, disturbance variables and aging phenomena in the measuring system are
almost completely eliminated, so that this measuring system, in contrast to other measuring
procedures, remains practically drift-free between calibrations and is entirely selective for CO

and

2

humidity. The sensor is built into the incubator and can be sterilized.
The O
The accuracy of the indicated values of CO

pressure (approx. 0.08 vol.-% per 10 mbar / 0.15 psi). To compensate for this effect in the CO

sensor is a semiconductor gas sensor with ZrO2 ceramic.

2

and O2 (unit with O2 control) depends on the ambient air

2

2

measurement, the controller measures the ambient air pressure and automatically includes it in the
calculation.

The CO

incubators CB are equipped with an Ethernet interface for computer communication, e.g. with

2

the communication software APT-COM™ 3 DataControlSystem (option, chap. 15.1). For further options,
see chap. 22.5.

Temperature range: 7 °C / 12.6 °F above ambient temperature up to +60 °C / 140 °F

range: 0 vol.-% up to 20 vol.-%)

CO

2

O

range (unit with O2 control): 0.2 vol.-% up to 95 vol.-%

2

CB (E6) 09/2013 Page 19/144

2.1 Unit overview

Instrument panel with

microprocessor controller

T4.12 and USB interface

Door handle

Unit door

Main power switch

Figure 3: CO2 incubator CB (example: model CB 160)

2.2 Instrument panel

5,7" controller display

Context-sensitive buttons

USB interface

Operating button

Pilot lamp: ready for operation

Figure 4: Instrument panel with microprocessor controller T4.12 and USB interface

CB (E6) 09/2013 Page 20/144

FRO NT

(A)

(I)
(
(
(

(B)
(C)
(D)
(E)

(G)

(H)

2.3 Inner chamber

J)
K)
L)

Figure 5: CB 160 with O

control and options

2

CB (E6) 09/2013 Page 21/144

(B)

(C)

(D)

(D2)
(F)

(G)

(E)

Figure 6: CB 160 / CB 220 with O

control and options

2

(A) Instrument panel with microprocessor controller T4.12, indicating temperature and CO

(unit with O2 control)”

O

2

(B) Connection socket for extra-low voltage supply (option, chap. 15.6)
(C) CO
(D) Gas mixing head CO
(D2) Additional gas mixing head O

and humidity sensor

2

2

/ N2 (unit with O2 control)

2

(E) Pt 100 temperature probe
(F) O

sensor (unit with O2 control)

2

(G) Internal socket 230V (max. 3 A) (option, chap. 15.4)
(H) Perforated shelves, made of stainless steel
(I) Measuring access port
(J) Inner glass doors
(K) Permadry™ water pans
(L) Lower housing cover

as well as

2

CB (E6) 09/2013 Page 22/144

2.4 Control panel on the rear of the unit

(1) (2) (3) (6) (4) (5)

(7) (8) (9) (10)

Figure 7: Rear control panel CB with O

control and options

2

(1) Miniature fuse
(2) External socket for extra-low voltage supply (option for CB 160 / CB 220, chap. 15.6)
(3) DIN socket for analog outputs 4-20 mA (option, chap. 15.5)
(4) Quick acting closure socket for CO
(5) Quick acting closure socket for O
(6) Quick acting closure socket for N

2

(unit with O2 control)

2

(unit with O2 control)

2

(7) Socket for IEC connector plug for power cable
(8) Ethernet interface for computer communication
(9) DIN-socket for zero-voltage relay alarm outputs
(10) RS 422 interface for computer communication (option)

CB (E6) 09/2013 Page 23/144

3. Completeness of delivery, transportation, storage, and installation

3.1 Unpacking, and checking equipment and completeness of delivery

After unpacking, please check the unit and its optional accessories, if any, based on the delivery receipt
for completeness and for transportation damage. Inform the carrier immediately if transportation damage
has occurred.

The final tests of the manufacturer may have caused traces of the shelves on the inner surfaces. This has
no impact on the function and performance of the unit.

Please remove any transportation protection devices and adhesives in/on the unit and on the doors and
remove the operating manuals and accessory equipment.

Remove any protective lamination sheet on the inner metal surfaces prior to commissioning.

CAUTION

Sliding or tilting of the unit.
Damage to the unit.
Risk of injury by lifting heavy loads.

 Do NOT lift or transport the unit using the door handle, the door or the lower housing.
 Lift the unit from the pallet at the four lower corners with the aid of four people.

If you need to return the unit, please use the original packing and observe the guidelines for safe lifting
and transportation (chap. 3.2).

For disposal of the transport packing, see chap. 20.1.

Note on second-hand units (Ex-Demo-Units):

Second-hand units are units that were used for a short time for tests or exhibitions. They are thoroughly
tested before resale. BINDER ensures that the chamber is technically sound and will work flawlessly.

Second-hand units are marked with a sticker on the unit door. Please remove the sticker before
commissioning the unit.

3.2 Guidelines for safe lifting and transportation

After operation, please observe the guidelines for temporary decommissioning (chap. 20.2). Empty the
Permadry™ water pan before moving the incubator. In case of any spilling of the contents, shut down the
incubator and dry it out carefully and completely.

CB (E6) 09/2013 Page 24/144

CAUTION

Sliding or tilting of the unit.
Damage to the unit.
Risk of injury by lifting heavy loads.

 Transport the unit in its original packaging only.
 For moving or shipping, secure the unit with transport straps.

∅ Do NOT lift or transport the unit using the door handle, the door or the lower housing.

 Lift the unit at the four lower corners with the aid of 4 people and place it on a rolling

pallet.

 Move the unit to the desired location and lift it from the rolling pallet with the aid of four

people.

• Permissible ambient temperature range during transport: 10 °C / 14 °F to +60 °C / 140 °F.

You can order transport packing for moving or shipping purposes from BINDER service.

3.3 Storage

Intermediate storage of the unit is possible in a closed and dry room. Observe the guidelines for
temporary decommissioning (chap. 20.2).

• Permissible ambient temperature range during storage: -10 °C / 14 °F to +60 °C / 140 °F.

• Permissible ambient humidity: max. 70 % r.H., non-condensing

When after storage in a cold location you transfer the unit to its warmer installation site, condensation
may form. Before start-up, wait at least one hour until the chamber has attained ambient temperature and
is completely dry.

3.4 Location of installation and ambient conditions

Notes on the location of installation

Set up the CO
The chambers are designed for setting up inside a building (indoor use).

Freestanding CB incubators are suitable for installation on tables or on the optionally available stand
(height 200 mm / 0.5 ft). Note: The site of installation must be capable of supporting the unit’s weight (see
technical data, chap.22.4).

Align the unit using a spirit level to ensure even covering of the cell-cultures with the medium. For this
purpose, manually adjust the four incubator feet.

incubators can be stacked on top of each other (two units maximum). For safe stacking that is easy

CO

2

to maintain, use the original BINDER stacking stand (chap. 15.8.1) or the stacking adapter (chap. 15.8.2).

incubator on a flat, even surface, free from vibration and in a well-ventilated, dry location.

2

CAUTION

Sliding of stacked unit.
Damage to the units.

 When stacking, use rubber pads (Art. No. 8012-0376) for the feet of the upper

incubator.

To completely separate the unit from the power supply, you must disconnect the power plug. Install the
unit in a way that the power plug is easily accessible and can be easily pulled in case of danger.

In order to avoid contamination, never place the unit directly on the floor.

CB (E6) 09/2013 Page 25/144

CAUTION

Danger of overheating.
Damage to the unit.

∅ Do NOT set up units in non-ventilated recesses.

 Ensure sufficient ventilation for dispersal of the heat.

Do not install or operate the CO2 incubator CB in potentially explosive areas.

DANGER

Explosion hazard.
Danger of death.

∅ Do NOT operate the unit in potentially explosive areas.

 KEEP explosive dust or air-solvent mixtures AWAY from the vicinity of the unit.

Ambient conditions

• Permissible ambient temperature range for operation: +18 °C / 64.4 °F to +30 °C / 86 °F

At elevated ambient temperature values, fluctuations in temperature can occur.

• Ideal ambient temperature: at least 7 °C / 12.6 °F below the intended working temperature. E.g.,

working temperature 37 °C / 98.6 °F = ambient temperature 30 °C / 86 °F and lower. In the event of
working temperatures of less than 7 °C / 12.6 °F above the ambient temperature, the setpoint can be
exceeded.

The ambient temperature should not be substantially higher than the indicated ambient
temperature of 22 ±3 °C / 71.6 ±5.4 °F to which the specified technical data relates. For other
ambient conditions, deviations from the indicated data are possible.

Avoid direct solar radiation on the unit.

• Permissible ambient humidity: 70 % r.H. max., non-condensing.

• Installation height: max. 2000 m / 6561.7 ft above sea level.

• Wall distances: rear 100 mm / 3.94 in, sides 50 mm / 1.97 in.

CB (E6) 09/2013 Page 26/144

Notes on handling carbon dioxide (CO

Carbon dioxide (CO

) in high concentrations is hazardous to health. It is colorless and almost odorless

2

and therefore practically imperceptible. Vent out any CO
or a suitable connection to an exhaust system. We recommend installing a CO

)

2

gas that may escape via good room ventilation

2

warning system.

2

WARNING

High concentration of CO2 (> 4 Vol.-%).
Danger of death by suffocation.
Danger of poisoning.

∅ Do NOT set up units in non-ventilated recesses.

 Ensure technical ventilation measures.
 Observe the relevant regulations for handling CO

Observe the occupational exposure limit OEL for CO2 set by the national authorities (formerly
maximum permitted workplace concentration). Check compliance when operating all units located in the
room.

3

• OEL for Germany: 5000 ml/m

• CO

lost with each opening the door: about 16.4 g, i.e. 0.0084 cubic meters / 0.296 cubic feet (under

2

(ppm) = 0,5 Vol.-%

normal pressure)

• CO

lost during 12h at 5 vol.-% without opening the door: approx. < 2 g, i.e. 0.001 cubic meter / 0.035

2

cubic feet (under normal pressure 1013 mbar / 14.7 psi)

.

2

An example of how to evaluate laboratory volume and air change rate:

Question: Is an air change rate of 1/h sufficient for a lab with a volume of 100 cubic meters /

3,531.5 cubic feet with 10 incubators CB, opened 4 times per hour?

Calculation: CO

concentration = CO2 lost by opening the door, multiplied by 10 units, multiplied

2

by opening the door 4 times per hour, divided by lab volume

0.0084 cubic meters x 10 x 4 div. 100 cubic meters = 0.00336, i.e. 0.336 % or 3360 ppm.

0.296 cubic feet x 10 x 4 div. 3,531.5 cubic feet = 0.00336, i.e. 0.336 % or 3360 ppm.
Result: The maximum permissible value of 5000 ppm is not exceeded under these operation

conditions.

Unit with O

Oxygen (O

control: Notes on handling oxygen (O2)

2

) is colorless and almost odorless and therefore practically imperceptible. It promotes burns,

2

which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes
and hair. O

is heavier than air and may accumulate in low-lying areas.

2

WARNING

High concentration of O2 (> 21 % O2).
Fire and explosion hazard through contact of combustible materials with O
Risk of burns and other injuries.

∅ Do NOT set up units in non-ventilated recesses.

 Ensure technical ventilation measures.
 Observe the relevant regulations for handling O

.

2

.

2

Take appropriate measures to prevent oxygen enrichment and fire and explosion hazards in areas where
oxygen enrichment is possible.

CB (E6) 09/2013 Page 27/144

t and necessary safety

Strictest smoking ban and no ignition sources in areas where oxygen enrichment is

from ignition sources (flames, cigarettes, etc.) and ventilate their clothes at least 15

General information for safe handling of oxygen:

• Make sure training of personnel on hazards of oxygen enrichmen
measures.

• Make sure adequate labeling of all oxygen equipment and facilities.

• Make sure gas tightness of all gas connections by checking them for leaks (e.g. with leak

spray or diluted soap solution).

• Close the main valve of the source of oxygen after work when not using the chamber.

• Never lubricate O

equipment with oil or fat. Use only materials and spare parts which are

2

approved for use with oxygen.

• Regularly inspect fire extinguishers for proper condition.

• Set up emergency showers where oxygen enrichment is possible.

•

possible.

• Make sure good ventilation of areas where oxygen enrichment is possible (location of the
chamber and/or O

cylinders.

2

• Persons who may have been in a possibly oxygen-enriched atmosphere must keep away

minutes.

• Always keep emergency routes free.

Unit with O

Nitrogen (N
therefore practically imperceptible. Any N

control: Notes on handling nitrogen (N2)

2

) in high concentrations is hazardous to health. It is colorless and almost odorless and

2

gas that may escape must be safely led out via good room

2

ventilation or a suitable connection to an exhaust system.

WARNING

High concentration of N2.
Risk of death by suffocation.

∅ Do NOT set up units in non-ventilated recesses.

 Ensure technical ventilation measures.
 Observe the relevant regulations for handling N

.

2

CB (E6) 09/2013 Page 28/144

Figure 8: Permadry™ water pan CB 160 / CB 220

Figure 9: Permadry™ water pan CB 60

4. Installation and connections

4.1 Shelves

You can put the shelves in different positions at the line of channel beads in the inner chamber. Hold the
shelf straight and then insert it so it will go smoothly inside the unit.

Permitted shelf loads:

Maximum load of one single shelf: 10 kg / 22 lb
Maximum total load of all shelves: 30 kg / 66 lb

4.2 Permadry™ water pan

The Permadry™ system developed by BINDER is an effective and easy to handle system that ensures
high humidity inside the incubator without any condensation forming on the inner surfaces. The
Permadry™ water pan consists of two pans in which the outer one is heated and the inner one cooled.
With the slight difference of temperature caused by that cooling, the central pan is the specific point for
condensation of the surplus humidity. Therefore, all other inner surfaces remain dry.

• Put the Permadry™ water pan on the bottom of the inner chamber in a way that both notches lock into

place.

• The front side of the Permadry™ water pan is marked “FRONT”.

Figure 10: Letters “FRONT” indicating the front of the Permadry™ water pan

• Please make sure that the Permadry™ water pan has firm contact to the inner chamber bottom and

rests tightly on it.

• Fill only the outer pan with distilled, sterilized water up to the filling level marking on the edge of the

inner pan.
Maximum filling quantity of the outer pan: CB 60: approx. 0.7 liters, CB 160 and CB220): approx.

2 liters.

Figure 11: Filling height line of the outer basin CB 160 / CB 220

CB (E6) 09/2013 Page 29/144

• We recommend cleaning and refilling the pans 2 to 3 times a week. For evacuation, remove the

Permadry™ water pan.

• We recommend using distilled, sterile water to achieve optimum growth results. Any corrosive damage

that may arise following the use of water of different quality or by additives is excluded from the liability
agreement.

• If required, you can add microbiologically inhibiting substances such as copper chips, copper sulfate

or ethylene diamine tetra-vinegar acid (EDTA) in a concentration of 1 to 5 mmol/l.

Empty the Permadry™ water pan before moving the incubator. In case of the contents spilling,
immediately shut down the incubator and dry it carefully and completely.

4.3 Gas connections

General information for safe handling of gas cylinders:

• Store and use gas cylinders only in well ventilated areas.

• Open the gas cylinder valve slowly to avoid pressure surges.

• Secure gas cylinders during storage and use against falling (chaining).

• Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them.

• Always close the valve even with apparently empty cylinders; screw on the cap when not in

use. Return gas cylinders with the valve closed.

• Do not open gas cylinders by force. Mark them when damaged.

• Protect gas cylinders against fire, e.g. do not store together with flammable liquids.

• Observe relevant regulations for dealing with gas cylinders.

Secure the gas cylinder against falling and other mechanical damage.

WARNING

Safety valve tearing off.
Sudden release of the stored pressure energy.
Risk of injury.

 Secure gas cylinders against falling (chaining).
 Transport gas cylinders with a cylinder cart.

The valve of the gas cylinder always must be closed before screwing on or unscrewing the gas hose.

WARNING

Opening the cylinder valve when the cylinder is not connected.
Sudden release of the stored pressure energy.
Risk of injury.

 Close the gas cylinder valve before connecting or removing the gas hose.

After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or
diluted soap solution).

CB (E6) 09/2013 Page 30/144