Binder CB 60-UL, CB 160 E6, CB 160, CB 220, CB 220-UL Operating Manual

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CB 60-UL

100-120V

9040-0089, 9140-0089

CB 60

230 V

O2 control

9040-0090, 9140-0090

CB 60-UL

100-120V

O2 control

9040-0091, 9140-0091

CB 60

230 V

Divided inner door

9040-0104, 9140-0104

CB 60-UL

100-120V

Divided inner door

9040-0105, 9140-0105

CB 60

230 V

O2 control

Divided inner door

9040-0106, 9140-0106

CB 60-UL

100-120V

O2 control

Divided inner door

9040-0107, 9140-0107

CB 160 (E6)

230 V

9040-0092, 9140-0092

CB 160-UL

100-120V

9040-0093, 9140-0093

CB 160 (E6)

230 V

O2 control

9040-0094, 9140-0094

CB 160-UL

100-120V

O2 control

9040-0095, 9140-0095

CB 160

230 V

Divided inner door

9040-0100, 9140-0100

CB 160-UL

100-120V

Divided inner door

9040-0101, 9140-0101

CB 160

230 V

O2 control

Divided inner door

9040-0102, 9140-0102

CB 160-UL

100-120V

O2 control

Divided inner door

9040-0103, 9140-0103

CB 220

230 V

9040-0096, 9140-0096

CB 220-UL

100-120V

9040-0097, 9140-0097

CB 220

230 V

O2 control

9040-0098, 9140-0098

CB 220-UL

100-120V

O2 control

9040-0099, 9140-0099

CB 220

230 V

Divided inner door

9040-0108, 9140-0108

CB 220-UL

100-120V

Divided inner door

9040-0109, 9140-0109

CB 220

230 V

O2 control

Divided inner door

9040-0110, 9140-0110

CB 220-UL

100-120V

O2 control

Divided inner door

9040-0111, 9140-0111

Operating Manual

CB (E6.1) CO CO

- Incubators

– Incubators with O2 control

with sterilizable NDIR sensor system for CO2 and microprocessor controller T4.12

Model Voltage Equipment Art. No.

CB 60

230 V 9040-0088, 9140-0088

BINDER GmbH

 Address: Post office box 102, 78502 Tuttlingen, Germany  Phone: +49 7462 2005 0  Fax: +49 7462 2005 100  Internet: http://www.binder-world.com  E-mail: info@binder-world.com  Service Hotline: +49 7462 2005 555  Service Fax: +49 7462 2005 93 555  Service E-Mail: service@binder-world.com  Service Hotline USA: +1 866 885 9794 or +1 631 2 24 4340 x3  Service Hotline Asia Pacific: +852 390 705 04 or +852 390 705 03  Service Hotline Russia and CIS: +7 495 988 15 16

Issue 07/2017 Art. No. 7001-0333

Contents

1. SAFETY .................................................................................................................. 6

1.1 Legal considerations ........................................................................................................................... 6

1.2 Structure of the safety instructions ...................................................................................................... 6

1.2.1 Signal word panel ..................................................................................................................... 6

1.2.2 Safety alert symbol ................................................................................................................... 7

1.2.3 Pictograms ................................................................................................................................ 7

1.2.4 Word message panel structure ................................................................................................. 8

1.3 Localization / position of safety labels on the chamber ...................................................................... 8

1.4 Type plate ......................................................................................................................................... 10

1.5 General safety instructions on installing and operating the CO2 incubator ...................................... 11

1.6 Precautions when working with gases .............................................................................................. 12

1.7 Precautions when handling gas cylinders ......................................................................................... 14

1.8 Intended use ..................................................................................................................................... 15

2. CHAMBER DESCRIPTION .................................................................................. 16

2.1 Chamber overview ............................................................................................................................ 17

2.2 Instrument panel ............................................................................................................................... 17

2.3 Inner chamber ................................................................................................................................... 18

2.4 Control panel on the rear of the chamber ......................................................................................... 20

3. COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND

INSTALLATION .................................................................................................... 21

3.1 Unpacking, and checking equipment and complet eness of delivery ................................................ 21

3.2 Guidelines for safe lifting and transportation..................................................................................... 21

3.3 Storage .............................................................................................................................................. 22

3.4 Location of installation and ambient condi tions ................................................................................ 22

4. INSTALLATION AND CONNECTIONS ............................................................... 26

4.1 Shelves.............................................................................................................................................. 26

4.2 Permadry™ water pan ...................................................................................................................... 26

4.3 Connecting the O2 sensor (chamber with O2 control) ....................................................................... 27

4.4 Gas connections ............................................................................................................................... 28

4.4.1 Connection of the CO2 gas cylinder ........................................................................................ 29

4.4.2 Connection of the O2 gas cylinder (chamber with O2 control and optional alternative control range 10 up to 95 vol.-% O

4.4.3 Connection of the N2 gas cylinder (chamber with O2 control)................................................. 31

4.4.4 Connecting the gas hose to the chamber rear (for CO2, O2, N2) ............................................ 32

4.4.5 Gas cylinder connection kits (option) ...................................................................................... 33

4.5 Electrical connection ......................................................................................................................... 34

4.6 Handling and aligning the divided inner door, gas proof (optional equipment) ................................. 35

) .................................................................................................. 30

5. START UP ............................................................................................................ 36

5.1 Equilibration time ............................................................................................................................... 36

5.2 Factory settings ................................................................................................................................. 36

6. FUNCTIONAL OVERVIEW OF THE T4.12 CHAMBER CONTROLLER ............. 37

6.1 Menu structure .................................................................................................................................. 38

6.1.1 General menu ......................................................................................................................... 38

6.1.2 Quick menu ............................................................................................................................. 39

6.1.3 User menu .............................................................................................................................. 40

6.2 Operating modes ............................................................................................................................... 41

6.2.1 Activating the “control off” mode or change to “f i xed value” operating mode ......................... 41

6.3 Deactivating the O2 control and O2/N2 pressure alarms (chamber with O2 control) ......................... 43

6.3.1 Required gas supply of the chamber with O2 control ............................................................. 43

6.3.2 Activating / deactivating the O2 control and O2 / N2 pressure alarms ..................................... 44

CB (E6.1) 07/2017 Page 2/145

6.4

Humidity control of the Permadry™ system ...................................................................................... 45

6.5 Turning on / off the interior socket voltage (wit h optional interior socket) ......................................... 46

6.6 Performance during and after power failure ...................................................................................... 46

6.7 Information ........................................................................................................................................ 47

7. SETPOINT ENTRY ............................................................................................... 48

7.1 Setting ranges ................................................................................................................................... 48

7.2 Note when setting high gas concentrations ...................................................................................... 48

7.3 Entering the setpoints via “quick menu” ............................................................................................ 50

7.4 Entering the setpoints via general menu ........................................................................................... 52

8. KEY LOCK ........................................................................................................... 54

8.1 Directly activating the key lock .......................................................................................................... 55

8.2 Automatic key lock ............................................................................................................................ 55

8.3 Changing the password for unlocking the key lock ........................................................................... 57

9. GENERAL CONTROLLER SETTINGS ................................................................ 58

9.1 Setup wizard ..................................................................................................................................... 59

9.2 Date and time settings ...................................................................................................................... 59

9.3 Selecting the menu language of the T4.12 controller ....................................................................... 61

9.4 Setting display brightness ................................................................................................................. 61

9.5 Changing the temperature unit ......................................................................................................... 62

9.6 Defining the data recording rate ........................................................................................................ 62

9.7 Factory reset ..................................................................................................................................... 63

9.8 Network configuration ....................................................................................................................... 63

9.9 Display of the network configuration ................................................................................................. 67

9.10 RS 422 address (with optional RS 422 interface) ............................................................................. 68

9.11 Display and entry of the device configuration – for se rvice purpose ................................................ 68

10. DATA TRANSFER VIA USB INTERFACE .......................................................... 69

10.1 Exporting data to USB drive .............................................................................................................. 69

10.2 Importing data from USB drive .......................................................................................................... 70

11. NOTIFICATIONS AND ALARMS ......................................................................... 71

11.1 Notifications overview ....................................................................................................................... 71

11.2 Alarm messages overview ................................................................................................................ 71

11.3 Alarm status ...................................................................................................................................... 73

11.4 Confirming an active “set” alarm ....................................................................................................... 74

11.5 Alarm configuration and overview ..................................................................................................... 75

11.5.1 List of active alarms ................................................................................................................ 75

11.5.2 History – list of all alarms ........................................................................................................ 76

11.5.3 Activating, deactivating, and testing the alarm buzzer ........................................................... 77

11.5.4 Activating / deactivating all alarm functions ............................................................................ 77

11.5.5 Setting the delay time after opening the door ......................................................................... 78

11.5.6 Tolerance ranges and alarm delay times ............................................................................... 78

11.6 Zero-voltage relay alarm output ........................................................................................................ 82

12. EVENT LIST ......................................................................................................... 83

13. GRAPHICAL DISPLAY OF THE MEASURED VALUES ..................................... 84

13.1 Setting the sampling rate .................................................................................................................. 84

13.2 Defining the display range................................................................................................................. 85

13.3 Selecting the parameters .................................................................................................................. 86

14. TEMPERATURE SAFETY DEVICES ................................................................... 87

14.1 Overtemperature protective device (class 1) .................................................................................... 87

14.2 Overtemperature safety controller (temperature safety device class 3.1) ........................................ 87

14.2.1 Safety controller modes .......................................................................................................... 87

14.2.2 Setting the safety controller .................................................................................................... 88

CB (E6.1) 07/2017 Page 3/145

15. OPTIONS .............................................................................................................. 91

15.1 Communication software APT-COM™ 3 DataControlSystem (option)............................................. 91

15.2 RS 422 interface (option) .................................................................................................................. 91

15.3 Silicone access ports 30 mm / 1.18 in, closable from both sides with silicon plugs (option) ............ 91

15.4 Interior socket 230V (option) ............................................................................................................. 92

15.5 Analog outputs for temperature and CO2 (option) ............................................................................ 94

15.6 Access port for extra-low voltage (option) ......................................................................................... 94

15.7 BINDER Gas Supply Service – External bottle cha nger for CO2, N2 or O2 (option) ......................... 95

15.8 Stands ............................................................................................................................................... 96

15.8.1 Stacking stand (option) ........................................................................................................... 96

15.8.2 Stacking adapter for direct thermal decoupled stac king (option) ........................................... 96

15.8.3 Base on castors (option) ......................................................................................................... 96

16. REFERENCE MEASUREMENTS ........................................................................ 97

16.1 CO2 reference measuring ................................................................................................................. 97

16.1.1 Measuring CO2 concentration indirectly via the pH of the cell m edium .................................. 97

16.1.2 Measuring CO2 directly via chemical indicator tubes ............................................................. 98

16.1.3 Measuring CO2 directly with an electronic infrared measuring device ................................... 99

16.2 Temperature reference measurement .............................................................................................. 99

17. AVOIDING MICROBIAL CONTAMINATION........................................................ 99

17.1 Cells and media ................................................................................................................................ 99

17.2 Laboratory conditions / equipment aroun d the incubator .................................................................. 99

17.3 Working and behavior in the lab ..................................................................................................... 100

17.4 Chamber design and equipment of the CO2 incubator ................................................................... 100

17.5 Handling the CO2 incubator ............................................................................................................ 101

18. CLEANING, DECONTAMINATION / DISINFECTION, AND STERILIZATION .. 103

18.1 Cleaning .......................................................................................................................................... 103

18.2 Decontamination / chemical disinfection of the chamber ................................................................ 105

18.3 Hot-air sterilization at 180 °C / 356 °F............................................................................................. 106

18.3.1 Overview ............................................................................................................................... 106

18.3.2 Performing a hot-air sterilization ........................................................................................... 107

18.3.3 Aborting the hot-air sterilization – general procedure ........................................................... 109

18.3.4 Aborting hot-air sterilization after less than 4 hours ............................................................. 110

18.3.5 Terminating successful sterilization during the cooling-down phase (abortion after more than

4 hours) ................................................................................................................................. 112

18.3.6 Terminating the completed sterilization cycle ....................................................................... 113

19. MAINTENANCE AND SERVICE ........................................................................ 115

19.1 Maintenance intervals, service ........................................................................................................ 115

19.2 Checking the air jacket heating fan ................................................................................................. 116

19.3 Checking the humidity system fan .................................................................................................. 116

19.4 Gas inlet fine filter ........................................................................................................................... 116

19.5 Sending the chamber back to BINDER GmbH ............................................................................... 116

20. DISPOSAL ......................................................................................................... 117

20.1 Disposal of the transport packing .................................................................................................... 117

20.1.1 Outer chamber packing ........................................................................................................ 117

20.1.2 Packing inside the chamber and equipment ......................................................................... 117

20.2 Decommissioning ............................................................................................................................ 118

20.3 Disposal of the chamber in the Federal Republic of Germany ....................................................... 118

20.4 Disposal of the chamber in the member states of the EU except for the Federal Republic of

Germany.......................................................................................................................................... 119

20.5 Disposal of the chamber in non-member states of the EU ............................................................. 120

CB (E6.1) 07/2017 Page 4/145

21. TROUBLESHOOTING ....................................................................................... 121

21.1 General............................................................................................................................................ 121

21.2 Heating ............................................................................................................................................ 121

21.3 Gas cylinder pressure too low ......................................................................................................... 123

21.4 Gas concentration ........................................................................................................................... 124

21.5 Sterilization ...................................................................................................................................... 126

21.6 Humidity .......................................................................................................................................... 126

21.7 Controller ......................................................................................................................................... 127

21.8 Open door ....................................................................................................................................... 127

22. TECHNICAL DESCRIPTION .............................................................................. 128

22.1 Factory calibration and adjustment ................................................................................................. 128

22.2 Over current protection ................................................................................................................... 128

22.3 Definition of usable volume ............................................................................................................. 128

22.4 CB technical data ............................................................................................................................ 129

22.5 Equipment and Options ................................................................................................................... 131

22.6 Accessories and spare parts (extract) ............................................................................................ 132

22.7 Important conversion data for non-SI units ..................................................................................... 133

22.8 Conversion table for gas inlet pressures, bar – psi ......................................................................... 133

22.9 Dimensions CB 60 .......................................................................................................................... 134

22.10 Dimensions CB 160 ........................................................................................................................ 135

22.11 Dimensions CB 220 ........................................................................................................................ 136

23. CERTIFICATES AND DECLARATIONS OF CONF ORMITY ............................. 137

23.1 EU Declaration of conformity .......................................................................................................... 137

24. PRODUCT REGISTRATION .............................................................................. 139

25. CONTAMINATION CLEARANCE CERTIFICATE ............................................. 140

25.1 For chambers located outside the USA and Canada ..................................................................... 140

25.2 For chambers in the USA and Canada ........................................................................................... 143

CB (E6.1) 07/2017 Page 5/145

Dear customer,

For the correct operation of the CO completely and carefully and observe all instructions as indicated. Failure to read, understand and follow the instructions may result in personal injury. It can also lead to damage to the chamber and/or poor equipment performance

incubator CB, it is important that you read this operating manual

1. Safety

This operating manual is part of the components of delivery. Always keep it handy for reference. The device should only be operated by laboratory personnel especially trained for this purpose and familiar with all precautionary measures required for working in a laboratory. Observe the national regulations on minimum age of laboratory personnel. To avoid injuries and damage observe the safety instructions of the operating manual.

WARNING

Failure to observe the safety instructions. Serious injuries and chamber damage.

 Observe the safety instructions in this operating manual.  Carefully read the complete operating instructions of the chamber.

1.1 Legal considerations

This operating manual is for informational purposes only. It contains information for installing, start-up, operation and maintenance of the product. Note: the contents and the product described are subject to change without notice.

Understanding and observing the instructions in this operating manual are prerequisites for hazard-free use and safety during operation and maintenance. In no event shall BINDER be held liable for any damages, direct or incidental arising out of or related to the use of this manual.

This operating manual cannot cover all conceivable applications. If you would like additional information, or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or contact us directly by phone at the number located on page one of this manual

Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or existing agreement, description, or legal relationship, nor do they modify such a relationship. All obligations on the part of BINDER derive from the respective purchase contract, which also contains the entire and exclusively valid statement of warranty administration. The statements in this manual neither augment nor restrict the contractual warranty provisions.

1.2 Structure of the safety instructions

In this operating manual, the following safety definitions and symbols indicate dangerous situations following the harmonization of ISO 3864-2 and ANSI Z535.6.

1.2.1 Signal word panel

Depending on the probability of serious consequences, potential dangers are identified with a signal word, the corresponding safety color, and i f appropriate, the safety alert symbol.

DANGER

Indicates an imminently hazardous situation that, if not avoided, will result in death or serious (irreversible) injury.

CB (E6.1) 07/2017 Page 6/145

CAUTION

Warning signs

Electrical hazard

Hot surface

Explosive atmosphere

poisoning hazard

or chemical burns

Biohazard

WARNING

Indicates a potentially hazardous situation which, if not avoided, could result in death or serious (irreversible) injury.

CAUTION

Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor (reversible) injury.

Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product and/or its functions or of a property in its prox i m i ty.

1.2.2 Safety alert symbol

Use of the safety alert symbol indicates a risk of injury. Observe all measures that are marked with the saf ety alert symbol in order to avoid death or

injury.

1.2.3 Pictograms

Lifting hazard

Explosive substances

Gas cylinders

Fire promoting agents

Suffocation hazard

Harmful substances

Stability hazard

suffocation and

Risk of corrosion and /

CB (E6.1) 07/2017 Page 7/145

Pollution Hazard

Mandatory action signs

plug

Environment protection

Prohibition signs

water

Pictograms (Warning signs)

Service label

Mandatory regulation

Do NOT touch

Information to be observed in order to ensure optim um function of the product.

Read operating

instructions

Wear protective gloves

Do NOT spray with

1.2.4 Word message panel structure

Type / cause of hazard. Possible consequences.

 Instruction how to avoid the hazard: prohibition  Instruction how to avoid the hazard: mandatory a ct i on

Disconnect the power

Wear safety goggles

Do NOT climb

Lift with several persons

Observe all other notes and information not necessarily emphasized in the same way, in order to avoid disruptions that could result in direct or indirect injury or property damage.

1.3 Localization / position of safety labels on t he chamber

The following labels are located on the chamber:

Hot surface

• on the outer chamber door Risk of injury

• on the outer door: CB-UL only

• above the access ports (option)

CB (E6.1) 07/2017 Page 8/145

CO2 -Incubator CB CO2 -Incubator CB-UL

Figure 1: Position of labels on the chamber

Keep safety labels complete and legible.

Replace safety labels that are no longer legible. Contact BINDER service for these replacements.

CB (E6.1) 07/2017 Page 9/145

Indications of the type plate (example)

Information

BINDER

Manufacturer: BINDER GmbH

CB 160

Model designation

CO2 incubator

Device name

Serial No.

00000000000000

Serial no. of the chamber

Built

2017

Year of construction

Nominal

187 °C 369 °F

IP protection

IP type of protection acc. to standard EN 60529

Temp. safety device

DIN 12880

Temperature safety device acc. to standard DIN 12880

Class

3.1

Class of temperature safety device

Art. No.

9040-0092

Art. No. of the chamber

Project No.

---

Optional: Special application acc. to project no.

1,30 kW

Nominal power

230 V / 50 Hz

230 V / 60 Hz

1 N ~

Current type

5,7 A

Nominal current

Symbol on the type plate

Information

Nominal temp.

187 °C

1,30 kW / 5,7 A

369 °F

230 V / 50 Hz

IP protection

230 V / 60 Hz

Safety device

DIN 12880

1 N ~

Class

3.1

Art. No.

9040-0092

Built

2017

CO2 incubator

BINDER GmbH

www.binder-world.com

CB 160

Serial No. 00000000000000

1.4 Type plate

Position of type plate: left chamber side (see n from front), at the bottom in the middle

Project No.

temperature

Im Mittleren Ösch 5 78532 Tuttlingen / Germany

E6.1

Made in Germany

Figure 2: Type plate (example of CB 160 regular chamber)

Nominal temperature

Nominal voltage ± 10% at the indicated power fre quency

CE conformity marking Electrical and electronic equipment manufactured / placed on

the market in the EC after 13 August 2005 and to be disposed of in separate collection according to Directive 2012/19/EU on waste electrical and electronic equipment (WEEE).

The chamber is certified by Underwriters Laboratories Inc. according to the following standards:

(CB-UL only)

CAN/CSA-C22.2 No. 61010-1, 2 UL 61010-1, 2

Edition, 2005-07-22

Edition, 2004-07

CB (E6.1) 07/2017 Page 10/145

1.5 General safety instructions on installing and operating the CO2 incubator

With regard to operating the chamber and to the installation location, please observe the guideline BGI/GUV-I 850-0 on safe working in laboratories (formerly BGR/GUV-R 120 or ZH 1/119 laboratory guidelines issued by the employers’ liabilit y insurance association) (for Germany).

BINDER GmbH is only responsible for the safety features of the chamber provided skilled electricians or qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating to chamber safety are replaced in the event of failure with original spare parts.

To operate the chamber, use only original BINDER accessories or accessories from third-party suppliers authorized by BINDER. The user is responsible for any risk caused by using unauthorized acces sories.

CAUTION

Danger of overheating. Damage to the chamber.

∅ Do NOT install the chamber in unventilated recesses.

 Ensure sufficient ventilation for dispersal of the heat.

Do not operate the chamber in hazardous locati ons.

DANGER

Explosion hazard. Danger of death.

∅ Do NOT operate the chamber in potentially explosive areas.

 KEEP explosive dust or air-solvent mixtures AWAY from the chamber.

The chamber does not dispose of any measures of explosion protection.

DANGER

Explosion hazard. Danger of death.

∅ Do NOT introduce any sub st ance into the chamber which is combustible or explosiv e at

working temperature.

∅ NO explosive dust or air-sol vent mixture in the inner chamber.

Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature inside the chamber must lie below the flash point or below the sublimation point of the charging material. Familiarize yourself with the physical and chemical properties of the charging material, as well as the contained moisture constituent and its behavior with the addition of heat energy.

Familiarize yourself with any potential health risks caused by the charging material, the contained moisture constituent or by reaction products which may arise during the temperature process. Take adequate measures to exclude such risks prior to putting the chamber into operation.

DANGER

Electrical hazard. Danger of death.

∅ The chamber must NOT become wet during operation or m ai ntenance.

The chambers were produced in accordance with VDE regulations and were routinely tested in accordance to VDE 0411-1 (IEC 61010-1).

CB (E6.1) 07/2017 Page 11/145

During and after a sterilization the temperature of the inner surfaces almost equals the set-point.

CAUTION

The inner doors, the inner door and glass door handles, and the inner cham ber will become hot during a sterilization.

Danger of burning.

∅ Do NOT touch the inner doors, the inner door and glass door handles, and the inner

surfaces after a sterilization.

WARNING

Stability hazard. Danger of injury. Damage to the chamber and the charging material. Housing cover breakaway.

∅ Do NOT climb on the lower housing cover. ∅ Do NOT load the lower housing cover with heavy objects while the chamber door is

open.

1.6 Precautions when working with gases

Notes on handling carbon dioxide (CO2)

Carbon dioxide (CO and therefore practically imperceptible. Vent out any CO or a suitable connection to an exhaust sy st em. We recommend installing a CO

) in high concentrations is hazardous to health. It is colorless and almost odorless

gas that may escape via good room ventilation

WARNING

High concentration of CO2 (> 4 Vol.-%). Risk of death by suffocation. Danger of poisoning.

∅ Do NOT set up chambers in non-ventilated recesses.

 Ensure technical ventilation measures.  Observe the relevant regulations for handling CO

warning system

CB (E6.1) 07/2017 Page 12/145

t and necessary safety

them for leaks (e.g. with leak

equipment with oil or fat. Use only materials and spare parts which are

Strictest smoking ban and no ignition sources in areas where oxygen enrichment is

areas where oxygen enrichment is possible (location of the

enriched atmosphere must keep away

Chamber with O

Oxygen (O

control: Notes on handling oxygen (O2)

) is colorless and almost odorless and therefore practically imperceptible. It promotes burns, which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes and hair. O

is heavier than air and may accumulate in low-lyin g areas.

WARNING

High concentration of O2 (> 21 % O2). Fire and explosion hazard through contact of combustible materials with O Risk of burns and other injuries.

∅ Do NOT set up chambers in non-ventilated recesses.

 Ensure technical ventilation measures.  Observe the relevant regulations for handling O

Take appropriate measures to prevent oxygen enrichment and fire and explosion hazards in areas where oxygen enrichment is possible.

General information for safe handling of oxy gen:

• Make sure training of personnel on hazards of oxygen enrichmen measures.

• Make sure adequate labeling of all oxygen equipment and facilities.

• Make sure gas tightness of all gas connections by checking

spray or diluted soap solution).

• Close the main valve of the source of oxygen after work when not using the chamber.

• Never lubricate O

approved for use with oxygen.

• Regularly inspect fire extinguishers for proper condition.

• Set up emergency showers where oxygen enrichment is possible.

•

possible.

• Make sure good ventilation of chamber and/or O2 cylinders.

• Persons who may have been in a possibly oxygenfrom ignition sources (flames, cigarettes, etc.) and ventilate their clothes at least minutes.

• Always keep emergency routes free.

Chamber with O2 control: Notes on handling nitrogen (N2)

Nitrogen (N

) in high concentrations is hazardous to health. It is colorless and almost odorless and

therefore practically imperceptible. Any N

gas t hat may escape must be safely led out via good room

ventilation or a suitable connection to an exhaust system.

WARNING

High concentration of N2. Risk of death by suffocation.

∅ Do NOT set up chambers in non-ventilated recesses.

 Ensure technical ventilation measures.  Observe the relevant regulations for handling N

CB (E6.1) 07/2017 Page 13/145

on the cap when not in

1.7 Precautions when handling gas cylinders

General information for safe handling of gas cy li nders:

• Store and use gas cylinders only in well-ventilated locations.

• Open the gas cylinder valve slowly to avoid pressure surges.

• Secure gas cylinders during storage and use against falling (chaining).

• Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them.

• Always close the valve even with apparently empty cylinders; screw

use. Return gas cylinders with the valve closed.

• Do not open gas cylinders by force. Mark them when damaged.

• Protect gas cylinders against fire, e.g. do not store together with flammable liquids.

• Observe relevant regulations for dealing with gas cylinders.

Secure the gas cylinders against falling and other mechanical damage.

WARNING

Safety valve tearing off. Sudden release of the stored pressure energy. Risk of injury.

 Secure gas cylinders against falling (chaining).  Transport gas cylinders with a cylinder cart.

The valve of the gas cylinder always must be clo sed before screwing on or unscrewing the gas hose.

WARNING

Opening the cylinder valve when the cylinder is not connected. Sudden release of the stored pressure energy. Risk of injury.

 Close the gas cylinder valve before connecting or removing the gas hose.

After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution).

CB (E6.1) 07/2017 Page 14/145

stible or explosive at working temperature into

1.8 Intended use

Series CB incubators are suitable for the cultivation of mammal cells under typical conditions of approx. 37 °C / 98.6°F. The chamber permits setting defined pH conditions by common NaHCO of commercial cell media by keeping an exact CO

atmosphere inside. The chambers guarantee high

humidity inside to avoid osmolarity increasin g caused by the evaporation of the cell media.

buffer systems

With the chamber w ith O

control, a variable oxygen atmosphere can additionally influence the growth of

the cells. The chambers are suitable for exact conditioning of harmless materials. Any possible solvent any solvent

must not be explosive and flammable. Components of the charging material must NOT form an explosive mixture with air. The operating temperature must lie below the flash point or below the sublimation point of the charging material. Any component of the charging material must NOT be able to release toxic gases.

DANGER

Explosion or implosion hazard. Danger of poisoning. Danger of death.

∅ Do NOT introduce any substance combu

the chamber, in particular no energy sources such as batteries or lithium-ion batteries.

∅ NO explosive dust or air-sol vent mixture in the inner chamber. ∅ Do NOT introduce any sub st ance which could lead to release of toxic gases.

Other applications are not approved.

Following the instructions in this operating manual and conducting regular maintenance work (chap. 19.1) are part of the intended use.

The chambers are not classified as medical devices as defined by the Medical Device Directive 93/42/EEC.

Due to the special demands of the Medical Device Directive (MDD), these chambers are not qualified for sterilization of medical devic es as defined by the directive 93/42/EWG.

WARNING: If customer should use a BINDER ch am ber running in non-supervised continuous operation, we strongly recomm end in case of inclusion of irrecoverable specime n or samples to split such specimen or samples a nd store them in at least two chambers, if this is feasible.

The charging material shall not contain any corrosive ingredients that may damage the machine components made of stainless steel, al um i num, and copper. Such ingredients include in particular acids and halides. Any cor rosive damage caused by such ingredients is excluded from liability by BINDER GmbH.

In case of foreseeable use of the chamber there is no risk for the user through the integration of the chamber into systems or by special environmental or operating conditions in the sense of EN 610101:2010. For this, the intended use of the chamber and all its connections must be observed.

CB (E6.1) 07/2017 Page 15/145

2. Chamber description

The CO2 incubators CB are equipped with a multifunctional microprocessor display controller for temperature, CO degree resp. 0.1 vol.-%

The inner chamber, the pre-heating chamber and the inside of the doors are all made of stainless steel V2A (German material no. 1.4301, US equivalent AISI 304). The inner surfaces are smooth and therefore easy to clean. The inner chamber is deep-drawn from one piece, polished (suitable for pharmaceutical applications) and has no welds or inaccessible corners. The hinges and the seal of the inner glass door are glued from the outside to aid cleaning of the inner chamber. When operating the chamber at high temperatures (sterilization), the impact of the oxygen in the air may cause discoloration of the metallic surfaces (yellowish-brown or blue) by natural oxidation processes. These colorations are harmless and will in no way impair the function or quality of the chamber.

The perforated shelves are also made of stainless steel. You can insert a maximum of 3 (CB 60), 6 (CB

160), resp. 8 (CB 220) shelves. The housing is RAL 7035 powder-coated. All corners and edges are also completely coated. The chamber’s heating system permits hot-air auto-sterilization at a setpoint of 187.5 °C / 369.5°F. Thus,

a temperature of 180 °C / 356°F is maintained for at least 30 minutes on all internal surfaces, resulting in sterilization of the entire inner chamber. Therefore, this procedure meets all international guidelines regarding hot air sterilization, e.g. AA M I ST63, DIN 58947, European Pharmacopoeia.

, and O2 (chamber with O2 control) levels and a digital display accurate to one-tenth of a

Thanks to the standard safety device (class 3.1 according to DIN 12880), the set temperature is maintained in case of failure.

The gas enters the chamber via a fine filter (aseptic filter) with a high filtration efficiency that also filters the smallest particles.

A highly precise, drift-free CO

gas through a special proprietary gas mixing head developed by BINDER allows precise and

constant CO The CO

concentrations for long periods. This creates optimum growth conditions for cultures.

incubator is also available with O2 control in addition to CO2 control. There are two different

infrared measuring system in combination with the permanent mixture of

control ranges:

• Regular equipment: Hypoxic control range 0.2 to 20 vol. % O concentration; it is not possible to connect O2 gas bottles to increase O2 concentration. Control in

the low O

• Alternative control range 10 to 95 vol. % O

range is very precise, in particular in the ra nge below 1 vol. % O2.

(option no. 8012-1106). Although the high control range is

intended in particular for hyperoxic applications (> 21 vol. % O applications between 10 and 20 vol. % O

and O2 sensors

Fast reaction times, maximum accuracy and selectivity characterize the CO the CB incubator series. The accuracy of the CO

measuring system is based on a double-beam

. Only N2 can be connected to reduce

), it is also suitable for slightly hypoxic

measuring procedure of

infrared measuring cell with NDIR (non-dispersive infrared) sensor, which continuously regulates to a reference value. Therefore, disturbance variables and aging phenomena in the measuring system are almost completely eliminated, so that this measuring system, in contrast to other measuring procedures, remains practically drift-free between calibrations and is entirely selective for CO

. The

sensor is built into the chamber and can be sterili zed. The O The accuracy of the indicated values of CO

sensor is a semiconductor gas sensor with Z rO2 ceramic.

and O2 (chamber with O2 control) depends on the

ambient air pressure (approx. 0.08 vol.-% per 10 mbar / 0.15 psi). To compensate for this effect in the

measurement, the controller measures the ambient air pressure and automatically includes it in

the calculation.

The chambers are equipped with an Ethernet interface for computer communication, e.g. with the communication software APT-COM™ 3 DataControlSystem (option, chap. 15.1). For further options, see chap. 22.5.

CB (E6.1) 07/2017 Page 16/145

Temperature range: 7 °C / 12.6 °F above ambient temperature up to +60 °C / 140 °F CO

range: 0 vol.-% up to 20 vol.-%)

range (chamber with O2 control): 0.2 vol.-% up to 20 vol.-% (hypoxic control range) or 10 Vol.-%

up to 95 Vol.-% (alternative control range)

2.1 Chamber overview

Instrument panel with microprocessor

controller T4.12 and USB interface

Door handle

Chamber door

Main power switch

Figure 3: CO2 incubator CB (example: model CB 160)

2.2 Instrument panel

5,7" controller display

Context-sensitive buttons USB interface

Operating button

Pilot lamp: ready for operation

Figure 4: Instrument panel with microprocess or controller T4.12 and USB interface

CB (E6.1) 07/2017 Page 17/145

(A)

(B) (C) (D) (

(G) (F)

(E) (

(I) ( ( (

2.3 Inner chamber

D2)

J) K)

Figure 5: CB 160 with O

control and options

CB (E6.1) 07/2017 Page 18/145

(B)

(G) (F)

(E)

Figure 6: CB 160 / CB 220 with O

control and options

(A) Instrument panel with microprocessor controller T4.12, indicating temperature and CO

(chamber with O2 control)”

(B) Connection socket for extra-low voltage supply (option, chap. 15.6) (C) CO (D) Gas mixing head CO (D2) Additional gas mixing head O

sensor

/ N2 (chamber with O2 control)

(E) Pt 100 temperature probe (F) O

sensor (chamber with O2 control)

(G) Internal socket 230V (max. 3 A) (option, chap. 15.4) (H) Perforated shelves, made of st ainl ess steel (I) Measuring access port (J) Inner glass doors (K) Permadry™ water pans (L) Lower housing cover

as well as

CB (E6.1) 07/2017 Page 19/145

2.4 Control panel on the rear of t he chamber

(1) (2) (3) (6) (4) (5)

(7) (8) (9) (10)

Figure 7: Rear control panel CB with O

control and options

(1) Miniature fuse (2) External socket for extra-low voltage supply (option for CB 160 / CB 220, chap. 15.6) (3) DIN socket for analog outputs 4-20 mA (option, chap. 15.5) (4) Quick acting closure socket for CO (5) Quick acting closure socket for O

10 up to 95 vol.-% O

)

(6) Quick acting closure socket for N

(chamber with O2 control and optional alternative control range

(chamber with O2 control)

(7) Socket for IEC connector plug for power cable (8) Ethernet interface for computer communication (9) DIN-socket for zero-voltage relay alarm outputs (10) RS 422 interface for computer communication ( option)

CB (E6.1) 07/2017 Page 20/145

3. Completeness of delivery, transportation, storage, and

installation

3.1 Unpacking, and checking equipm ent and completeness of delivery

After unpacking, please check the chamber and its optional accessories, if any, based on the delivery receipt for completeness and for transportation damage. Inform the carrier immediately if transportation damage has occurred.

The final tests of the manufacturer may have caused traces of the shelves on the inner surfaces. This has no impact on the function and performance of the chamber.

Please remove any transportation protection devices and adhesives in/on the chamber and on the doors and remove the operating manuals and accessory equipment.

Remove any protective lamination sheet on the inner metal surfaces prior to commissioning.

CAUTION

Sliding or tilting of the chamber. Damage to the chamber. Risk of injury by lifting heavy loads.

 Do NOT lift or transport the chamber using the doo r handle, the door or the lower

housing.

 Lift the chamber from the pallet at the four lower corners with the aid of four people.

If you need to return the chamber, please use the original packing and observe the guidelines for safe lifting and transportation (chap. 3.2).

For disposal of the transport packing, see chap. 20.1.

Note on second-hand chambers (Ex-Demo-Units):

Second-hand chambers are chambers that were used for a short time for tests or exhibitions. They are thoroughly tested before resale. BINDER ensures that the chamber is technically sound and will work flawlessly.

Second-hand chambers are marked with a sticker on the chamber door. Please remove the sticker before commissioning the chamber.

3.2 Guidelines for safe lifting and t ransport ation

After operation, please observe the guidelines for temporary decommissioning (chap. 20.2). Empty the Permadry™ water pan before moving the chamber. In case of any spilling of the contents, shut down the chamber and dry it out carefully and completely.

CB (E6.1) 07/2017 Page 21/145

CAUTION

Sliding or tilting of the chamber. Damage to the chamber. Risk of injury by lifting heavy loads.

 Transport the chamber in its original packaging only.  For moving or shipping, secure the chamber with transport straps.

∅ Do NOT lift or transport the chamber using the door handle, the door or the lower

housing.

 Lift the chamber at the four lower corners with the aid of 4 peopl e and place it on a

rolling pallet.

 Move the chamber to the desired location and lif t it from the rolling pallet with the aid of

four people.

• Permissible ambient temperature range during transport: 10 °C / 14 °F to +60 °C / 140 °F.

You can order transport packing for moving or shipping purposes from BINDER service.

3.3 Storage

Intermediate storage of the chamber is possible in a closed and dry room. Observe the guidelines for temporary decommissioning (chap. 20.2).

• Permissible ambient temperature range during storage: -10 °C / 14 °F to +60 °C / 140 °F.

• Permissible ambient humidity: max. 70 % r.H., non-condensing

When after storage in a cold location you transfer the chamber to its warmer installation site, condensation may form. Before start-up, wait at least one hour until the chamber has attained ambient temperature and is completely dry.

3.4 Location of installation and ambi ent conditions

Notes on the location of installation

Set up the chamber on a flat, even surface, free from vibration and in a well-ventilated, dry location. The chambers are designed for setting up inside a building (indoor use).

Freestanding chamber are suitable for installation on tables or on the optionally available stand (height 200 mm / 0.5 ft). Note: The site of installation must be capable of supporting the chamber’s weight (see technical data, chap.22.4).

Align the chamber using a spirit level to ensure even covering of the cell-cultures with the medium. For this purpose, manually adjust the four chamber feet.

The chambers can be stacked on top of each other (two chambers maximum). For safe stacking that is easy to maintain, use the original BINDER stacking stand (chap. 15.8.1) or the stacking adapter (chap.

15.8.2).

CAUTION

Sliding of stacked chamber. Damage to the chambers.

 When stacking, use rubber pads (Art. No. 8012-0376) for the feet of the upper

chamber.

To completely separate the chamber from the power supply, you must disconnect the power plug. Install the chamber in a way that the power plug is ea sily accessible and can be easily pulled in case of danger.

For the user there is no risk of temporary ov ervoltages in the sense of EN 61010-1:2010.

CB (E6.1) 07/2017 Page 22/145

CAUTION

In order to avoid contamination, never place the chamber directly on the floor.

Danger of overheating. Damage to the chamber.

∅ Do NOT set up chambers in non-ventilated recesses.

 Ensure sufficient ventilation for dispersa l of the heat.

Do not install or operate the chamber in potentially explosive areas.

DANGER

Explosion hazard. Danger of death.

∅ Do NOT operate the chamber in pot entially explosive areas.

 KEEP explosive dust or air-solvent mixtures AWAY from the vicinity of the chamber.

Ambient conditions

• Permissible ambient temperature range for operation: +18 °C / 64.4 °F to +30 °C / 86 °F

At elevated ambient temperature values, f l uct uations in temperature can occur.

• Ideal ambient temperature: at least 7 °C / 12.6 °F below the intended working temperature. E.g.,

working temperature 37 °C / 98.6 °F = ambient temperature 30 °C / 86 °F and lower. In the event of working temperatures of less than 7 °C / 12.6 °F above the ambient temperature, the setpoint can be exceeded.

The ambient temperature should not be substanti al ly higher than the indicated ambient temperature of 22 ±3 °C / 71.6 ±5.4 °F to which the specified technical data relates. For other

ambient conditions, deviations from the indicated data are possible.

Avoid direct solar radiation on the chamber.

• Permissible ambient humidity: 70 % r.H. max., non-condensing.

• Installation height : max. 2000 m / 6561.7 ft above sea level.

• Wall distances: rear 100 mm / 3.94 in, sides 50 mm / 1.97 in.

CB (E6.1) 07/2017 Page 23/145

Notes on handling carbon dioxide (CO

Carbon dioxide (CO

) in high concentrations is hazardous to health. It is colorless and almost odorless

and therefore practically imperceptible. Vent out any CO or a suitable connection to an exhaust sy st em . We recommend installing a CO

)

gas that may escape via good room ventilation

warning system.

WARNING

High concentration of CO2 (> 4 Vol.-%). Danger of death by suffocation. Danger of poisoning.

∅ Do NOT set up chambers in non-ventilated recesses.

 Ensure technical ventilation measures.  Observe the relevant regulations for handling CO

Observe the occupational exposure limit OEL for CO2 set by the national authorities (formerly maximum permitted workplace concentration). Check compliance when operating all chambers located in the room.

• OEL for Germany: 5000 ml/m

• CO

lost with each opening the door: about 16.4 g, i.e. 0.0084 cubic meters / 0.296 cubic feet (under

(ppm) = 0,5 Vol.-%

normal pressure)

• CO

lost during 12h at 5 vol.-% without opening the door: approx. < 2 g, i.e. 0.001 cubic meter / 0.035

cubic feet (under normal pressure 1013 mbar / 14.7 psi)

An example of how to evaluate laboratory volume and air change rate:

Question: Is an air change rate of 1/h sufficient for a lab with a volume of 100 cubic meters /

3,531.5 cubic feet with 10 incubators CB, opened 4 times per hour?

Calculation: CO

concentration = CO2 lost by opening the door, multiplied by 10 chambers,

multiplied by opening the door 4 times per hour, di vided by lab volume

0.0084 cubic meters x 10 x 4 div. 100 cubic meters = 0.00336, i.e. 0.336 % or 3360 ppm.

0.296 cubic feet x 10 x 4 div. 3,531.5 cubic feet = 0.00336, i.e. 0.336 % or 336 0 ppm. Result: The maximum permissible value of 5000 ppm is not exceeded under these operation

conditions.

Even when CO

or systems operated with CO2 are handled carefully and appropriately, a residual risk

remains, which can lead to life-threatening situations under certain circumstances. Therefore we strongly recommend continuous monitoring of CO be ensured permanently that the maximum permissible occupational exposure limit OEL for CO % CO

for Germany) is not exceeded.

concentration in the ambient air of the CO2 incubator. It must

(0.5 vol -

Chamber with O

Oxygen (O

control: Notes on handling oxygen (O2)

is heavier than air and may accumulate in low-lyin g areas.

WARNING

High concentration of O2 (> 21 % O2). Fire and explosion hazard through contact of combustible materials with O

Risk of burns and other injuries.

∅ Do NOT set up chambers in non-ventilated recesses.

 Ensure technical ventilation measures.  Observe the relevant regulations for handling O

CB (E6.1) 07/2017 Page 24/145

t and necessary safety

all gas connections by checking them for leaks (e.g. with leak

parts which are

Strictest smoking ban and no ignition sources in areas where oxygen enrichment is

Make sure good ventilation of areas where oxygen enrichment is possible (location of the

enriched atmosphere must keep away

ventilate their clothes at least 15

Take appropriate measures to prevent oxygen enrichment and fire and explosion hazards in areas where oxygen enrichment is possible.

General information for safe handling of oxygen:

• Make sure training of personnel on hazards of oxygen enrichmen measures.

• Make sure adequate labeling of all oxygen equipment and facilities.

• Make sure gas tightness of

spray or diluted soap solution).

• Close the main valve of the source of oxygen after work when not using the chamber.

• Never lubricate O

equipment with oil or fat. Use only materials and spare

approved for use with oxygen.

• Regularly inspect fire extinguishers for proper condition.

• Set up emergency showers where oxygen enrichment is possible.

•

possible.

• chamber and/or O2 cylinders.

• Persons who may have been in a possibly oxygenfrom ignition sources (flames, cigarettes, etc.) and minutes.

• Always keep emergency routes free.

Chamber with O

Nitrogen (N therefore practically imperceptible. Any N

control: Notes on handling nitrogen (N2)

) in high concentrations is hazardous to health. It is colorless and almost odorless and

gas that may escape must be safely led out via good room

ventilation or a suitable connection to an exhaust system.

WARNING

High concentration of N2. Risk of death by suffocation.

∅ Do NOT set up chambers in non-ventilated recesses.

 Ensure technical ventilation measures.  Observe the relevant regulations for handling N

CB (E6.1) 07/2017 Page 25/145

CB 60

CB 160 / CB 220

4. Installation and connections

4.1 Shelves

You can put the shelves in different positions at the line of channel beads in the inner chamber. Hold the shelf straight and then insert it so it will go smoothl y inside the chamber.

Permitted shelf loads:

Maximum load of one single shelf: 10 kg / 22 lb Maximum total load of all shelves: 30 kg / 66 lb

4.2 Permadry™ water pan

The Permadry™ system developed by BINDER is an effective and easy to handle system that ensures high humidity inside the chamber without any condensation forming on the inner surfaces. The Permadry™ water pan consists of two pans in which the outer one is heated and the inner one cooled. With the slight difference of temperature caused by that cooling, the central pan is the specific point for condensation of the surplus humidity. Therefo re, all other inner surfaces remain dry.

Figure 8: Permadry™ water pan

• Put the Permadry™ water pan on the bottom of the inner chamber in a way that both notches lock into

place.

• The front side of the Permadry™ water pan is marked “FRONT”.

Figure 9: Letters “FRONT” indicating the front of the Permadry™ water pan

• Please make sure that the Permadry™ water pan has firm contact to the inner chamber bottom and

rests tightly on it.

• Fill only the outer pan with distilled, sterilized water up to the filling level marking on the edge of the

inner pan. Maximum filling quantity of the outer pan: CB 60: approx. 0.7 liters, CB 160 and CB220): approx.

2 liters.

Figure 10: Filling height line of the outer basin CB 160 / CB 220

CB (E6.1) 07/2017 Page 26/145

• We recommend cleaning and refilling the pans 2 to 3 times a week. For evacuation, remove the

Permadry™ water pan.

• We recommend using distilled, sterile water to achieve optimum growth results. Any corrosive damage

that may arise following the use of water of different quality or by additives is excluded from the liability agreement.

• If required, you can add microbiologically inhibiting substances such as copper chips, copper sulfate

or ethylene diamine tetra-vinegar acid (EDTA) in a concentration of 1 to 5 mmol/l.

Empty the Permadry™ water pan before moving the chamber. In case of the contents spilling, immediately shut down the chamber and dry it careful ly and completely.

4.3 Connecting the O2 sensor (chamber with O2 control)

The O2 sensor is supplied with the chamber in a separate package.

Connect or remove the O2 sensor only when the chamber is turned off.

Open the door of the inner chamber and plug the O2 sensor (F) into the left connection socket located in the upper part of the rear of the inner chamber. P ay attention to the correct positioning of the pins.

Figure 11: O2 sensor Figure 12: Connecting the O2 sensor

The O2 sensor must be plugged in during a hot-air sterilization.

CB (E6.1) 07/2017 Page 27/145

Always close the valve even with apparently empty cylinders; screw on the cap when not in

4.4 Gas connections

General information for safe handling of gas cy l i nders:

• Store and use gas cylinders only in well ventilated areas.

• Open the gas cylinder valve slowly to avoid pressure surges.

• Secure gas cylinders during storage and use against falling (chaining).

• Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them.

•

use. Return gas cylinders with the valve closed.

• Do not open gas cylinders by force. Mark them when damaged.

• Protect gas cylinders against fire, e.g. do not store together with flammable liquids.

• Observe relevant regulations for dealing with gas cylinders.

Secure the gas cylinder against falling and other m echanical damage.

WARNING

Safety valve tearing off. Sudden release of the stored pressure energy. Risk of injury.

 Secure gas cylinders against falling (chaining).  Transport gas cylinders with a cylinder cart.

The valve of the gas cylinder always must be clo sed before screwing on or unscrewing the gas hose.

WARNING

Opening the cylinder valve when the cylinder is not connected. Sudden release of the stored pressure energy. Risk of injury.

 Close the gas cylinder valve before connecting or removing the gas hose.

After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution).

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4.4.1 Connection of the CO2 gas cylinder

Carbon dioxide (CO2) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Vent out any CO or a suitable connection to an exhaust sy st em . We recommend installing a CO

High concentration of CO2 (> 4 Vol.-%). Risk of death by suffocation. Danger of poisoning.

∅ Do NOT set up chambers in non-ventilated recesses.

 Ensure technical ventilation measures.  Observe the relevant regulations for handling CO

The CO2 gas necessary for operation must have a t echnical grade of 99.5 %.

The gas connections must be established by quali fied personnel who are trained in handling the respective gases and familiar with the required safety measures.

gas that may escape via good room ventilation

WARNING

warning system.

The following steps are required:

Ensuring the correct CO

output pressure

A gas supply pressure above 2.5 bar / 36 psi will result in chamber damage.

Use a pressure reducer and make sure to avoid any excessive outlet pressure when connecting the gas hose to the chamber.

The real outlet pressure of gas cylinders, sets of gas cylinders or central gas supplies am on the second manometer must not exceed 2.5 bar / 36 psi.

CAUTION

Excessive outlet pressure > 2.5 bar / 36 psi. Damage to the chamber.

∅ The outlet pressure must NOT exceed the indicated value of 2.5 bar / 36 psi.

 Before connecting, check the outlet pressure on the pressure reducer of the

cylinder.

 Adjust the outlet pressure to 2.0 bar / 29 psi above the ambient pressure.

Observe the correct outlet pressure also when replacing t he gas cylinders.

Establishing the connection to the chamber

Connect the supplied gas hose (internal diameter 6 mm / 0.24 inches) to the pressure reducer of the gas cylinders or central gas supply and secure the connection with the supplied hose clamp.

Connect the pre-assembled hose nozzle of the gas hose to the quick acting closure socket (4) DN 6 on the chamber rear, as described in chap. 4.4.4.

Leak test

After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution).

CB (E6.1) 07/2017 Page 29/145

cylinder or disconnect the gas supply (by pulling off the gas hose)

CAUTION

The recovery times of the gas concentrations inside the chamber after opening the door are indicated in the technical data (chap. 22.4) and refer to a connecti on pressure of 2.0 bar / 29 psi. Decreasing supply pressure will result in l onger recovery times.

Conversion table for gas inlet pressures, bar – psi, see chap. 22.8.

4.4.2 Connection of the O2 gas cylinder (chamber with O2 control and optional alternative control range 10 up to 95 vol.-% O

Note: Do not connect the O2 when operating at setpoints below 19 vol.-% O2.

Oxygen (O2) is colorless and almost odorless and therefore practically imperceptible. It promotes burns, which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes and hair. O

is heavier than air and may accumulate in low-lyin g areas.

)

WARNUNG

High concentration of O2 (> 21 % O2). Fire and explosion hazard through contact of combustible materials with O Risk of burns and other injuries.

∅ Do NOT set up chambers in non-ventilated recesses.

 Ensure technical ventilation measures.  Observe the relevant regulations for handling O

The O2 gas necessary for operation must have a t echnical grade of 99.5 %.

The gas connections must be established by quali fied personnel who are trained in handling the respective gases and familiar with the required safety measures.

The following steps are required:

Ensuring the correct O

output pressure

A gas supply pressure above 2.5 bar / 36 psi will result in chamber damage.

Use a pressure reducer and make sure to avoid any excessive outlet pressure when connecting the gas hose to the chamber.

The real outlet pressure of gas cylinders, sets of gas cylinders or central gas supplies am on the second manometer must not exceed 2.5 bar / 36 psi.

Excessive outlet pressure > 2.5 bar / 36 psi. Damage to the chamber.

∅ The outlet pressure must NOT exceed the indicated value of 2.5 bar / 36 psi.

 Before connecting, check the outlet pressure on the pressure reducer of the

cylinder.

 Adjust the outlet pressure to 2.0 bar / 29 psi above the ambient pressure.

Observe the correct outlet pressure also when replacing t he gas cylinders.

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Establishing the connection to the chamber

Connect the supplied gas hose (internal diameter 6 mm / 0.24 inches) to the pressure reducer of the gas cylinders or central gas supply and secure the connection with the supplied hose clamp.

Connect the pre-assembled hose nozzle of the gas hose to the quick acting closure socket (5) DN 6 on the chamber rear, as described in chap. 4.4.4.

Leak test

After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution).

Conversion table for gas inlet pressures, bar – psi, see chap. 22.8.

4.4.3 Connection of the N2 gas cylinder (chamber with O2 control)

Nitrogen (N2) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore practically imperceptible. Any N ventilation or a suitable connection to an exhaust system.

gas that may escape must be safely led out via good room

WARNING

High concentration of N2. Risk of death by suffocation.

∅ Do NOT set up chambers in non-ventilated recesses.

 Ensure technical ventilation measures.  Observe the relevant regulations for handling N

The N2 gas necessary for operation must have a t echnical grade of 99.5 %.

The gas connections must be established by quali fied personnel who are trained in handling the respective gases and familiar with the required safety measures.

The following steps are required:

Ensuring the correct N

output pressure

A gas supply pressure above 2.5 bar / 36 psi will result in chamber damage.

Use a pressure reducer and make sure to avoid any excessive outlet pressure when connecting the gas hose to the chamber.

The real outlet pressure of gas cylinders, sets of gas cylinders or central gas supplies am on the second manometer must not exceed 2.5 bar / 36 psi.

CB (E6.1) 07/2017 Page 31/145

CAUTION

Excessive outlet pressure > 2.5 bar / 36 psi. Damage to the chamber.

∅ The outlet pressure must NOT exceed the indicated value of 2.5 bar / 36 psi.

 Before connecting, check the outlet pressure on the pressure reducer of the

cylinder.

 Adjust the outlet pressure to 2.0 bar / 29 psi above the ambient pressure.

Observe the correct outlet pressure also when repla cing the gas cylinders.

Establishing the connection to the chamber

Connect the supplied gas hose (internal diameter 6 mm / 0.24 inches) to the pressure reducer of the gas cylinders or central gas supply and secure the connection with the supplied hose clamp.

Connect the pre-assembled hose nozzle of the gas hose to the quick acting closure socket (6) DN 6 on the chamber rear, as described in chap. 4.4.4.

Leak test

After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or diluted soap solution).

Conversion table for gas inlet pressures, bar – psi, see chap. 22.8.

4.4.4 Connecting the gas hose to the chamber rear (for CO2, O2, N2)

The procedure of connecting the gas hose to the chamber rear is the same for any gas connection. All quick acting closure sockets (CO and optional alternative control range 10 up to 95 vol.-% O

, N2 on chamber with O2 control, and O2 on chamber with O2 control

) are degreased and supplied with a FKM

gasket.

Note for chambers with O

connection (chamber with O

control: The quick acting closure socket and the hose nozzle for the O2

control) must be degreased.

WARNING

Fire and explosion hazard through contact of fat with O2. Risk of burns and other injuries.

∅ All connection parts for the O

connection must be degreased.

The gas hose, which will be used to establish the connection to a gas cylinder, is already attached to the hose nozzle and secured by a hose clamp. Plug the hose nozzle into the corresponding quick acting closure socket (a) located at the rear of the chamber. This quick acting closure socket is closed by a rubber cover (b).

Only use the supplied hose nozzle to connect to t he qui ck acting closure socket. Otherwise, the quick acting closure socket may leak, and/or it may become impossible to

connect the original hose nozzle. In this case, pl ease contact BINDER Service.

CB (E6.1) 07/2017 Page 32/145

Gas cylinder connection kits are available for CO2 ( Art. No. 8012-0014), O2

rs for cylinder pressure (high pressure

assembled hose nozzle for quick acting

(a)

(c)

(b)

Remove the rubber cover (b) by pulling it of f.

Chamber rear

Figure 13: Connecting the hose lead to the gas cylinder

Now fit the hose nozzle (c) in the quick acting closure socket. To remove the connection, pull the hose nozzle off the quick acting closure socket.

4.4.5 Gas cylinder connection kits (option)

(Art. No. 8012-0015) and N2 (Art. No. 8012-0016). The connection kit includes the following parts for connecting a gas cylinder

to the CO

• Pressure reducer with manomete gauge) and outlet pressure (low pressure gaug e)

• 5 m pressure hose with preclosure socket

• 1 hose clamp to connect the gas hose to the pressure reducer

Figure 14: Gas cylinder connection kit

Instructions 7001-0249 included with the connection kit describe connecting and setting the gas cylinder connection kit.

incubator:

Excessive outlet pressure > 2.5 bar / 36 psi. Damage to the chamber.

∅ The outlet pressure must NOT exceed the indicated value of 2.5 bar / 36 psi.

 Before connecting, check the outlet pressure on the pressure reducer of the cylinder.  Adjust the outlet pressure to 2.0 bar / 29 psi above the ambient pressure.

The gas connections must be established by quali fied personnel who are trained in handling the respective gases and familiar with the required safety measures.

CB (E6.1) 07/2017 Page 33/145

CAUTION

4.5 Electrical connection

The chambers are supplied ready for connection. They come with an IEC connec tor plug. Model (including

chambers with O2 control and/or divided inner door)

Art. No. (x = 0 or 1)

Power plug

Voltage +/-10 %

Power frequency

Chamber fuse

CB 60

CB 60-UL

CB 160

CB 160-UL

CB 220

CB 220-UL

9x40-0088 9x40-0090

9x40-0089 9x40-0091

9x40-0092 9x40-0100 9x40-0094 9x40-0102

9x40-0093 9x40-0101 9x40-0095 9x40-0103

9x40-0096 9x40-0108 9x40-0098 9x40-0110

9x40-0097 9x40-0109 9x40-0099 9x40-0111

Shock-proof plug 200-240 V (1N~) 50/60 Hz 10 A

NEMA 5-15P 100-120 V (1N~) 50/60 Hz 16 A

Shock-proof plug 200-240 V (1N~) 50/60 Hz 10 A

NEMA 5-20P 100-120 V (1N~) 50/60 Hz 16 A

Shock-proof plug 200-240 V (1N~) 50/60 Hz 10 A

NEMA 5-20P 100-120 V (1N~) 50/60 Hz 16 A

• The domestic socket must also provide a protective conductor. Make sure that the connection of the protective conductor of the domestic installations to the chamber’s protective conductor meets the latest technology. The protective conduc tors of the socket and plug must be compatible!

• Prior to connection and start-up, check the power supply voltage. Compare the values to the specified data located on the chamber’s type plate (left chamber side, bottom right-hand, see chap. 1.4). We recommend the use of a residual current cir cuit breaker.

• When connecting, please observe the regulations specified by the local electricity supply company as well as the VDE directives (for Germany).

• Pollution degree (acc. to IEC 61010-1): 2

• Over-voltage category (acc. to IEC 61010-1): II

CAUTION

Danger of incorrect power supply voltage. Damage to the equipment.

 Check the power supply voltage before connect i on and start-up.  Compare the power supply voltage with the data indicated on the type plate.

5. Start up

After connecting the supply lines (chap. 4.5) turn on the chamber by the main power switch. The pilot lamp shows the chamber is ready for operation.

Observe a delay time of about 30s between turning Off and On again. Otherwise an initialization problem may occur.

Note that the chamber is in stand-by mode when the main power switch has been turned on and yet the controller display is dark. Turn on the chamber by pressing any controller button.

Warming chambers may release odors in the first few days after commissioning. This is not a quality defect. To reduce odors quickly we recommend heating up the chamber to its nominal temperature for one day and in a well-ventilated location.

WARNING: If customer should use a BINDER cham ber running in non-supervised continuous operation, we strongly recommend in case of i nclusion of irrecoverable specimen or samples to split such specimen or samples and store them in at least two chambers, if this is feasible.

After chamber startup this symbol and the message window “Power supply was interrupted.” appear in the controller display. Confirm with OK to close the window and make the icon disappear.

5.1 Equilibration ti m e

Temperature: Equilibration time is approx. 1 hour. CO

: After approx. 5 minutes, the CO2 concentration equilibrates automatically to the pre-set value of 5

vol.-% CO

(chamber with O2 control): After a delay of 10 minutes, the ambient oxygen concentration of approx.

20.7 vol.-% is displayed. During the first 10 minutes, O

control is not defined. Then O2 is regulated to the

displayed setpoint value of 20.7 vol.-%.

5.2 Factory settings

The chamber is supplied with the following basic preset parameter s:

• Temperature setpoint 37 °C / 98.6°F

• CO

• O

• Safety controller class 3.1 38.5 °C / 101.3°F

• Sterilization tem perature 187.5 °C / 369.5°F

concentration 5 vol.-%

concentration (chamber with O2 control) 20.7 vol.-%

• Audible alarm signal ( buzzer) turned on

The set temperature determines the target working temperature in the inner chamber, i.e. set value 37 °C / 98.6°F = target working temperature 37 °C / 98.6°F. The same is valid for the CO (chamber with O

control). For the hot-air sterilization, the set value is 187.5 °C / 369.5°F and cannot be

and O2 concentration

changed.

As long as there is a difference between the actual and set value shown in the display, adequate operation of the chamber is not guara nteed.

CB (E6.1) 07/2017 Page 36/145

6. Functional overview of the T4.12 chamber controller

The T4.12 chamber controller controls the following values inside the chamber:

• Temperature in °C (range by 7 °C / 12.6 °F above ambi ent temperature up to +60 °C / 140 °F)

• Carbon dioxide conc entration in vol.-% (range 0 vol.-% up to 20 vol.-%)

Chamber with O

• Oxygen concentration i n vol.-% (range 0.2 vol.-% up to 95 vol.-%)

control in addition:

You can enter the desired setpoint values in fixed value operating mode in the display controller. The controller also offers various notifications and alarm messages with visual and audible indication, a trace file and remote alarms via e -mail. Programming can be done directly through the keypad of the controller or graphically through the software APT-COM™ 3 DataControlSystem (option, chap. 15.1) specially developed by BINDER.

Temperature values

values

Actual values Setpoint values

Icons: Controller operating in “fixed value” operating mode

Figure 16: T4.12 microprocessor controller, initial view in “Fixed value” controller mode

(sample values, chamber with O

control)

CB (E6.1) 07/2017 Page 37/145

Fixed value

Setpoint

Actual value

37.0

37.2

5.0

20.7

Fixed value

..\ Menu

Controller mode

Event list

Alarms

Setpoints

Safety controller

Import/Export

Hot-air sterilization

Humidity control

6.1 Menu structure

08.03.2013 05:05:06

Temperature [°C] CO2 [Vol.-%] O2 [Vol.-%]

User Quick menu

From the Initial view you have access to different menus using the menu buttons “User”, “Quick menu”, or “Menu”. From there you can access the desired control functions. To do this, select the function by turning the operating button and press the oper ating button to confirm the selection.

In any menu, you can return to the previous display pressing the "Close" button or to the initial view with the "Home" button.

Depending on the logged-in user or administrator, the available menu functions may vary. These instructions present the functions which are available to the logged-in administrator.

6.1.1 General menu

The general menu provides access to all setting functions of the controller, a graphical display of the measured values, and the possibility to read and give out data via the USB interface. In addition, supporting functions like a settings wizard or a contact page are available.

Initial view (sample values, chamber with O2 control).

Press the desired menu button.

08.03.2013 05:05:06

General menu

Close Home

Turn the operating button to see additional menu items.

CB (E6.1) 07/2017 Page 38/145

Fixed value

..\ Menu

Settings

Measurement chart

Interior socket (optional)

Sensor adjustment

Service contact

System information

Fixed value

..\ Quick menu

Measurement chart

Active alarms

Temperature setpoint

CO2 setpoint

O2 setpoint

Safety controller setpoint

Hot-air sterilization

08.03.2013 05:05:06

General menu (next page)

Close Home

Controller mode

Switching between the operating modes “control of f” or “fixed value”, chap.

6.2.1 Event list Display of status information and errors, chap. 12 Alarms Setpoints Safety controller Import/Export Hot-air sterilization Humidity control Settings Measurement chart Interior socket (optional) Sensor adjustment Service contact System information

Alarm settings, chap. 11.5 Setpoint entry, chap. 7.4 Setting the safety controller, chap. 14.2 Data transfer via USB interface, chap. 10 Performing a hot-air sterilization at 180 °C / 356 °F, chap. 18.3 Setting the humidity control of the Permadry™ sy stem, chap.6.4 General controller settings, chap. 9 Graphical display of the measured values, chap. 13 Turning on / off the interior socket voltage (opt i on), chap. 6.5 Adjustment menu for single-point and two-point adjustments (for Service) Service information Chamber information (model, name, serial no., firmware etc.)

6.1.2 Quick menu

The Quick menu provides fast access to frequently used functions.

Close Home

CB (E6.1) 07/2017 Page 39/145

08.03.2013 05:05:06

“Quick menu”. (item “O

setpoint” only coming with

chamber with O

control)

Fixed value

..\ User

Key lock

Show event list

Measurement chart Graphical display of the measured values, chap. 13 Active alarms Alarm settings, chap. 11.5 Temperature setpoint Temperature setpoint entry, chap. 7.3 CO2 setpoint CO2 setpoint entry, chap. 7.3 O2 setpoint O2 setpoint, chap. 7.3 Safety controller setpoint Setting the safety controller setpoint, chap. 14.2.2 Hot-air sterilization Performing a hot-air sterilization at 180 °C / 356 °F, chap. 18.3

6.1.3 User menu

The user menu includes the key lock function, and pr ovides quick access to the event list. The key lock function serves to block the access to the controller. An overview of logon, logoff, and other

events is given in the event list.

08.03.2013 05:05:06

“User” menu

Close Home

Key lock Configuring the key lo ck function, chap. 8 Show event list Displaying the event list, chap. 12

CB (E6.1) 07/2017 Page 40/145

Fixed value

..\ Menu

Controller mode

Event list

Alarms

Setpoints

Safety controller

Import/Export

Hot-air sterilization

Humidity control

Fixed value

..\ Controller mode

Control off

Fixed value

O2 control

6.2 Operating modes

In the “control off” mode (chap. 6.2.1), the controller is non-functional and displays only the actual values. There is no heating or refrigeration and no inlet of CO temperature approximates the ambient val ue.

You can enter the desired setpoint values in “fixed value” mode (chap. 7). The controller then operates as a fixed-point control, i.e., it reaches a nd m ai ntains the defined setpoints until the next manual change.

6.2.1 Activating the “control off” mode or change to “fixed value” operating mode

To select the “control off” or “fixed value” operating m ode, go to Menu > Controller mode

08.03.2013 05:05:06

General menu. Select “Controller Mode”

and press the operating button.

or O2 (chamber with O2 control). The

Close Home

08.03.2013 05:05:06

Close Home

Submenu “Controller Mode”. Select the desired controller mode

“Control off” or “Fixed value” and press the operating button.

CB (E6.1) 07/2017 Page 41/145

Control off

..\ Menu

Controller mode

Event list

Alarms

Setpoints

Safety controller

Import/Export

Hot-air sterilization

Humidity control

Control off

Setpoint

Actual value

22.2

0.3

0.5

08.03.2013 05:05:06

General menu. The controller mode “Fixed value” or “Control off” is

indicated in the display headline.

Close Home

Go back to the initial view with “Home”.

08.03.2013 05:05:06

Temperature [°C]

CO2 [Vol.-%]

O2 [Vol.-%]

User Quick menu

The controller is non-functional, i.e., there is no heating or CO2 entry or O2 entry (chamber with O2 control).

Initial view in “Control off” mode (sample v al ues)

CB (E6.1) 07/2017 Page 42/145

Hyperoxic range

(>22 vol.-% O2)

Hypoxic range

(<20 vol.-% O2)

Hyperoxic and

hypoxic ranges

6.3 Deactivating the O2 control and O2/N2 pressure alarms (chamber with O2

control)

6.3.1 Required gas supply of the chamber with O2 control

In the chamber with O2 control, cell growth can be additionally influenced by a variable oxygen content. Depending on the required range of O2 concentr ation, the type of gas which is required may vary:

• Hyperoxic range (> 22 vol.-% O As compared to the ambient air (O

increased, no nitrogen is required for the O cylinder. You can deactivate the N

)

concentration of 20.9 vol.-%) only the O2 concentration needs to be

control. Therefore, it is not necessary to connect a N2

pressure alarm in the menu “Menu > Controller mode > O2 control”

(chap. 6.3.2).

• Hypoxic range (< 20 Vol.-% O

As compared to the ambient air (O be increased, no oxygen is required for the O cylinder. You can deactivate the O

)

concentration of 20.9 vol.-%) only the N2 concentration needs to

pressure alarm in the menu “Menu > Controller mode > O2

control. Therefore, it is not necessary to connect an O2

control” (chap. 6.3.2).

• Hyperoxic and hypoxic range or range from 20 vol.-% O

and O2 are required for O2 control. It is necessary to connect all gas sup pl i es.

• Operation without O

You can deactivate the O With this setting, no alarm messages for O

control

control in the menu “Menu > Controller mode > O2 control” (chap. 6.3.2).

and N2 (pressure or concentration) will be issued.

up to 22 vol.-% O2

Since neither nitrogen nor oxygen are required, it is not necessary to connect an O

and N2 pressure alarms will be issued with deactivated O2 control.

The zero-voltage relay alarm contact (chap. 11.6) for messages of deviation of the O will also not be triggered.

or N2 cylinder. No

concentration

Overview

No O2 control

off O2 control (with O2 concentration alarm)

active active active

Connection of O2 cylinder connected not connected connected not connected

off

O2 pressure alarm active

active off

Connection of N2 cylinder not connected connected connected not connected

off

N2 pressure alarm

active active off

CB (E6.1) 07/2017 Page 43/145

Fixed value

..\ O2 control

O2 control: On

O2 pressure alarm: On

N2 pressure alarm: On

Fixed value

Setpoint

Actual value

37.0

37.2

5.0

20.9

Fixed value

Setpoint

Actual value

37.0

37.2

5.0

20.9

6.3.2 Activating / deactivating the O2 control and O2 / N2 pressure alarms

To activate or deactivate the O2 control and O2 / N2 pressure alarms, go to

Menu > Controller mode > O2 control

08.03.2013 05:05:06

Close Home

Go back to the initial view with “Home”.

08.03.2013 05:05:06

Temperature [°C] CO2 [Vol.-%] O2 [Vol.-%]

Submenu “O2 control”. The current settings are displayed. To change the settings, press the operating button. The modified settings are displayed

“O2 control: On” = O “O2 control: Off” = O “O2 pressure alarm: On” or “N2 pressure alarm: O n”

= the corresponding pressure alarm is activated. “O2 pressure alarm: Off” or “N2 pressure alarm: Off”

= the corresponding pressure alarm is deactiv ated.

Initial view with deactivated O2 control (sample values).

Also the pressure alarms for O deactivated.

control is active.

control is deactivated.

and N2 are

User Quick menu

To enter the O2 setpoint, first activate the O2 control again.

With active O

Temperature [°C] CO2 [Vol.-%] O2 [Vol.-%]

User Quick menu

control, the pressure alarms for O2 and N2 can be deactivated individually.

08.03.2013 05:05:06

Initial view with activated O2 control and deactivated

pressure alarm (sample values).

CB (E6.1) 07/2017 Page 44/145

Fixed value

..\ Humidity control Low/Medium/High

Humidity control Medium

Fixed value

..\ Humidity control

Low

Medium

High

6.4 Humidity control of the Permad ry™ system

The Permadry™ system with its 2-pan water system guarantees a maximum humidity of up to 95 % r.H. in the inner chamber, which remains condensation-free. This performance assumes an average ambient temperature of 22 ±3 °C / 71.6 ±5.4 °F and a working temperature in the inner chamber of 37 °C /

98.6 °F. You can increase or decrease humidity slightly if required. To configure the humidity control of the Perm adry™ system, go to Menu > Humidity control

08.03.2013 05:05:06

Submenu “Humidity control”. The current setting is displayed. To change the setting, press the operating button.

Factory default setting: “Medium”.

Close Home

08.03.2013 05:05:06

Close Home

If the ambient temperature deviates by more than +/- 5 °C from the values recommended by the manufacturer, the conditions for maxi m um ai r humidity with condensation-free inner chamber are no longer guaranteed. Contact B INDER Service for assistance.

Submenu “Humidity control”. Select the desired setting

and press the operating button

CB (E6.1) 07/2017 Page 45/145

Fixed value

..\ Interior socket

Interior socket: Off

6.5 Turning on / off the interior socket vol tage (with optional interior socket)

For chambers equipped with the optional interior socket (chap. 15.4) you can turn on and off the voltage of the interior socket.

To turn on or off the interior socket voltage, go to Menu > Interior socket (optional)

08.03.2013 05:05:06

Submenu “Interior socket”. The current setting is displayed. To change the setting, press the operating button “Interior socket: On” = socket with activated voltage

“Interior socket: Off” = socket voltage-free

Close Home

Go back to the initial view with “Home”.

This symbol on the controller display indicates that the interior socket is activated.

6.6 Performance during and af ter power failure

During a power failure, all controller functions are shut down. The gas inlet valves are closed so that no gas can escape into the ambient air. The zero-voltage relay alarm output (9) (chap. 11.6) is switched to alarm position for the whole duration of the po wer f ai lure.

After the power returns, all functions return to the same status the chamber had before power failure. The controller continues to function in the original operating mode it was in previously before the power failure occurred. In “fixed value” mode, the setpoints a re i mmediately resumed.

If the chamber had been running in sterilization mode, the sterilization process is cancelled and the chamber operates in “fixed value” operation mode with the setpoints already entered. All setpoint values remain in memory.

If the temperature or concentration of CO alarm limits during power failure, confirm the alarms with the RESET button as soon as the correct values are reached again (chap. 11.4).

Power failure, including chamber shut-down with the main power switch, is listed in the event list.

, and O2 (chamber w ith O2 control) have dropped below the

CB (E6.1) 07/2017 Page 46/145

After the power returns or chamber startup, thi s sym bol and the message window “Power supply was interrupted.” appear in the controller display. Confirm with OK to close the window and make the icon disappear.

Fixed value

..\ System information

Chamber type: CB

Chamber name: CB_E6.1

Serial number: 00-00000

Special application number: 00-0000

Parameter version: 511B-0004-0000

Firmware version (1): 521C-0001-002E

Firmware version (2): 521B-0005-0023

Fixed value

..\ Service contact

Service hotline

International:

+49 7462 2005 555

USA Toll Free:

+ 1 866 885 9794 or

+ 1 631 224 4340

CIS:

+ 7 495 988 1516

service@binder-world.com

www.binder-world.com

6.7 Information

You access chamber information like the chamber type, serial no., firmware version etc. To display the system information, go to Menu > System information

08.03.2013 05:05:06

Submenu “System information” (sample values).

Close Home

To display the BINDER Service contact data, go to Menu > Service contact

08.03.2013 05:05:06

Submenu “Service contact”.

Close Home

Further information windows are accessible under Menu > Settings > Network settings > Show

network settings (chap. 9.9) and – for Service purpose – under Menu > Settings > Chamber configuration (chap. 9.11).

CB (E6.1) 07/2017 Page 47/145

7. Setpoint entry

7.1 Setting ranges

Temperature CO2

7 °C / 12.6 °F above ambient up to 60 °C / 140 °F 0 vol.-% up to 20 vol.- %

O2 (chamber with O2 control) 0.2 vol.-% up to 95 vol.-%

When changing the temperature setpoint, check the setting of the overtemperature safety controller class 3.1 (chap. 14.2).

With setpoint type “Limit”, adapt the safety controller always when changing the temperature setpoint.

When setting a lower temperature setpoint, i n order to save time, we recommend cooling down the chamber by turning it off and opening both chamber doors.

When setting a lower CO chamber doors for this purpose.

setpoint, the CO2 gas must be able to escape first. Open both

7.2 Note when setting high gas concentrati ons

Notes on handling carbon dioxide (CO2)

Carbon dioxide (CO and therefore practically imperceptible. Vent out any CO or a suitable connection to an exhaust sy st em. We recommend installing a CO

) in high concentrations is hazardous to health. It is colorless and almost odorless

gas that may escape via good room ventilation

warning system.

WARNING

High concentration of CO2 (> 4 Vol.-%). Danger of death by suffocation. Danger of poisoning.

∅ Do NOT set up chambers in non-ventilated recesses.

 Ensure technical ventilation measures.  Observe the relevant regulations for handling CO

If CO2 is released, leave the area und inform the security service or fire department.

Chamber with O

Oxygen (O

control: Notes on handling oxygen (O2)

is heavier than air and may accumulate in low-lyin g areas.

CB (E6.1) 07/2017 Page 48/145

WARNING

High concentration of O2 (> 21 % O2). Fire and explosion hazard through contact of combustible materials with O

Risk of burns and other injuries.

∅ Do NOT set up chambers in non-v entilated recesses

 Ensure technical ventilation measures  Observe the relevant regulations for handling O

Take appropriate measures to prevent oxygen enrichment and fire and explosion hazards in areas where oxygen enrichment is possible.

Observe the general information for safe handling of oxygen (chap. 1.6).

Chamber with O

Nitrogen (N therefore practically imperceptible. Any N

control: Notes on handling nitrogen (N2)

) in high concentrations is hazardous to health. It is colorless and almost odorless and

gas that may escape must be safely led out via good room

ventilation or a suitable connection to an exhaust system.

High concentration of N2. Risk of death by suffocation.

∅ Do NOT set up chambers in non-ventilated recesses

 Ensure technical ventilation measures  Observe the relevant regulations for handling N

WARNING

CB (E6.1) 07/2017 Page 49/145

Fixed value

..\ Quick menu

Measurement chart

Active alarms

Temperature setpoint

CO2 setpoint

O2 setpoint

Safety controller setpoint

Hot-air sterilization

[°C]

7.3 Entering the setpoints vi a “quick menu”

To enter setpoints via quick menu, go to Quick m enu.

08.03.2013 05:05:06

“Quick menu”. (item “O2 setpoint” only coming with

control)

Close Home

When trying to enter a setpoint in the „Controller off“ operating mode, a notification window shows “Controller mode is OFF!”. Press the operating button to confirm with “Ok” and change the operating mode to “Fixed value” (chap. 6.2.1).

chamber with O Select the desired parameter

and press the operating button.

Temperature setting

To enter the temperature setpoint, go to Quick m en u > Temperature setpoint

Fixed value

08.03.2013 05:05:06

..\ Temperature setpoint

Entry menu “Temperature setpoint”.

End Ok 0 1 2 4 5 6 7 8 9 Ins

Select each number with the operating button and press the operating button to confirm.

Setting range: 20 °C / 68 °F up to 60 °C / 140 °F. Press the “Ok” button to confirm.

Close Ok Home

When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.

Go back to the initial view with “Home” or enter the CO

concentration.

CB (E6.1) 07/2017 Page 50/145

Fixed value

..\ CO2 setpoint

[Vol.-%]

Fixed value

..\ O2 setpoint

[Vol.-%]

Setting the CO

To enter the CO

concentration

setpoint, go to Quick menu > CO2 setpoint

08.03.2013 05:05:06

Entry menu “CO2 setpoint”.

End Ok 0 1 2 3 4 6 7 8 9 Ins Del

Select each number with the operating butt on and press the operating button to confirm.

Setting range: 0 vol.-% up to 20 vol.-%. Press the “Ok” button to confirm.

Close Ok Home

When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.

Go back to the initial view with “Home” or ente r the O

concentration (chamber with O2 control).

Setting the O

To enter the O

concentration (chamber with O2 control)

setpoint, go to Quick menu > O2 setpoint

08.03.2013 05:05:06

Entry menu “O2 setpoint”.

End Ok 0 1 2 3 4 6 7 8 9 Ins Del

Select each number with the operating butt on and press the operating button to confirm.

Setting range: 0.2 vol.-% up to 95 vol.-%. Press the “Ok” button to confirm.

Close Ok Home

When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.

Go back to the initial view with “Home”.

CB (E6.1) 07/2017 Page 51/145

Fixed value

..\ Setpoints

Temperature setpoint

CO2 setpoint

O2 setpoint

Fixed value

..\ Temperature setpoint

[°C]

7.4 Entering the setpoints vi a gener al m enu

To enter setpoints via general menu, go to Menu > Setpoints

08.03.2013 05:05:06

Submenu “Setpoints”. (item “O2 setpoint” only coming with chamber with

control)

Select the desired parameter and press the operating button.

Close Home

Temperature setting

To enter the temperature setpoint, go to Menu > Setpoints > Temperature setpoint

08.03.2013 05:05:06

Entry menu “Temperature setpoint”.

End Ok 0 1 2 4 5 6 7 8 9 Ins

Select each number with the operating butt on and press the operating button to confirm.

Setting range: 20 °C / 68 °F up to 60 °C / 140 °F. Press the “Ok” button to confirm.

Close Ok Home

When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.

Go back to the initial view with “Home” or enter the CO

concentration.

CB (E6.1) 07/2017 Page 52/145

Fixed value

..\ CO2 setpoint

[Vol.-%]

Setting the CO

To enter the CO

concentration

setpoint, go to Menu > Setpoints > CO2 setpoint

08.03.2013 05:05:06

Entry menu “CO2 setpoint”.

End Ok 0 1 2 3 4 6 7 8 9 Ins Del

Select each number with the operating button and press the operating button to confirm.

Setting range: 0 vol.-% up to 20 vol.-%. Press the “Ok” button to confirm.

Close Ok Home

When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.

Go back to the initial view with “Home” or enter t he O

concentration (chamber with O2 control).

Setting the O

To enter the O

Fixed value

concentration (chamber with O2 control)

setpoint, go to Menu > Setpoints > O2 setpoint

08.03.2013 05:05:06

..\ O2 setpoint

Entry menu “O2 setpoint”.

Pos1 End Ok 0 1

3 4 5 6 7 8 9

Select each number with the operating button and press the operating button to confirm.

Setting range: 0.2 vol.-% up to 95 vol.-%. Press the “Ok” button to confirm.

Close Ok Home

When entering a value outside the setting range, the message “invalid value” appears. Press the operating button to confirm with “Ok” and repeat the entry with a correct value.

Go back to the initial view with “Home”.

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Fixed value

..\ User

Key lock

Show event list

Home

Fixed value

..\ Key lock

Key lock On

Automatic key lock

Password

8. Key lock

The key lock function serves to block the access to the controller. When the “key lock” function is activated, the controller changes to the initial view and can only be changed when entering the current password.

To configure the key lock function, go to User > Key lock

08.03.2013 05:05:06

“User” menu. Select “Key lock”

and press the operating button.

08.03.2013 05:05:06

Submenu “Key lock”. Select the desired function

and press the operating button.

Close Home

Key lock On The key lock is directly activated Automatic key lock The key lock is activated automatically aft er a defined waiting time Password Change password for unlocking. Factory set ting: 0000

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Fixed value

..\ Key lock

Key lock On

Automatic key lock

Password

Enter password

..\ Key lock password

1 2 3 4 5 6 7 8 9

Fixed value

..\ Automatic key lock

Automatic key lock

Waiting time [min]

8.1 Directly activating the key lock

To directly activate the key lock, go to User > Key lock > Key lock On

08.03.2013 05:05:06

Submenu “Key lock”. Select “Key lock On” to activate the key lock function

immediately and press the operating button.

Close Home

This symbol on the controller displays indicate s t hat the “key lock” function is activated.

The controller remains in the initial view and may be operated only after entering the current password.

08.03.2013 05:05:06

Ok: A B C D E F

Entry menu “Key lock password”. Enter the password with the operating button.

Factory setting is 0000 Press the “Ok” button to confirm.

Close Ok Home

8.2 Automatic key lock

To configure the automatic key lock, go to User > Key lock > Automatic key lock

08.03.2013 05:05:06

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Submenu “Automatic Key lock”. Select the desired function

and press the operating button.

Close Home

Fixed value

..\ Automatic key lock On/Off

Automatic key lock : Off

Fixed value

Setpoint

Actual value

37.0

37.2

5.0

25.0

Under “Waiting time [min]” you can enter the waiting time, after which the key lock will be automatically activated. This time starts running off af ter t he last entry to the controller. To enter it, go to

User > Key lock > Automatic key lock > Waiting time [min]

Enter wait time [min]

..\ User\Waiting time

Pos1 End Ok 0 2 3 4 5 6 7 8 9

Close Ok Home

To activate the automatic key lock functi on with the pre-configured waiting time, select

User > Key lock > Automatic key lock > Automatic key lock

08.03.2013 05:05:06

Entry menu “Waiting time”. Enter the desired interval with the operating button.

This interval starts running off after the last action on the controller. If the automatic key lock fu nct i on is enabled, it will become active after this time.

Factory setting: 1 minute. Press the “Ok” button to confirm.

Submenu “Automatic Key lock On/Off”. The current setting is displayed. To change the setting, press the operating button.

“Automatic Key lock : On” = Automatic key lock function activated. The time set under “Waiting time” begins running off.

“Automatic Key lock : Off” = Automatic key lock function deactivated

Close Home

Now the waiting time starts running off. Go back to the initial view with “Home”.

08.03.2013 05:05:06

Temperature [°C]

CO2 [Vol.-%]

O2 [Vol.-%]

User Quick menu

The controller remains in the initial view and may be operated only after entering the current password. After further entries to the controller, the waiting period begins running again, since the automatic keylock

function remains active until turning it off m anual l y.

Initial view. As soon as the waiting period has expired, the “key

lock” symbol is displayed.

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Fixed value

..\ Key lock

Key lock On

Automatic key lock

Password

Fixed value

..\ Key lock \ Change password

Do not change

Change password

..\ Key lock password

1 2 3 4 5 6 7 8 9

This symbol on the controller displays indicates that the “key lock” function is activated.

8.3 Changing the password f or unlocki ng t he key lock

To change the password for unlocking the key lock, go to User > Key lock > Password

08.03.2013 05:05:06

Submenu “Key lock”. Select „Password“

and press the operating button.

Close Home

A security question is displayed:

08.03.2013 05:05:06

Close Home

08.03.2013 05:05:06

Ok: A B C D E F

Close Ok Home

Submenu “Change password”. To change the password,

select “Change password” and press the operating button.

Entry menu “Key lock password”. Enter the desired password with the operating

button. Factory setting is 0000 Press the “Ok” button to confirm.

Keep well in mind any password modification. Without the correct password, unlocking the key lock is impossible.

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Fixed value

al menu

..\ Settings

Setup wizard

Date and time

Sprache, Language, Langue, Idioma, Lingua

Display brightness

Temperature unit

Recording rate

Factory reset

Network settings

Home

Fixed value

..\ Settings

Chamber configuration

RS 422 address (optional)

9. General controller settings

In the "Settings" submenu, you can enter the date and time, select the language for the menus and the desired temperature unit, perform the configuration for the controller’s communication functions, and reset the controller to factory settings.

To access the "Settings" submenu, go to Menu > Settings

08.03.2013 05:05:06

Submenu “Settings”. Turn the operating button to see addition

items.

08.03.2013 05:05:06

Submenu “Settings” (next page).

Close Home

Setup wizard Chap. 9.1 Date and time Setting date and time, chap. 9.2 Sprache, Language, Langue,

Idioma, Lingua

Selecting the controller’s menu language, chap. 9.3

Display brightness Adjusting display bright ness by turning the operating button, chap. 9.4 Temperature unit Selecting the temperature unit, chap.9.5 Recording rate Defining the recording rat e for data storage, chap. 9.6

Factory reset Factory reset, chap. 9.7 Network settings Network configuration, chap. 9.8

Chamber configuration

RS 422 address (optional)

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Menu for Service purpose – Entering chamber data like the serial no., service information

Setting the RS 422 chamber address(with option RS422 interface), chap. 9.10

Fixed value

..\ Date and time

Set date

Set time

9.1 Setup wizard

The setup wizard will guide you sequentially through the important menus to configure your chamber

• Menu language

• Device name

• Date and time

• IP address

• Subnet mask

• Network name

• Gateway

• DNS 1

• DNS 2

Then the controller returns to the initial view.

Use the setup wizard only if you want to enter all the requested information, as no item can be skipped.

You can configure the network settings (IP address, and the following) only if the DHCP status is Off, otherwise the DHCP server would assign the network configuration.

If you try configuring any network settings (i.e., when reaching item “IP address” in the setup wizard) while DHCP is enabled, the message "DHCP enabled!” is shown. After confirming with Ok the Setup wizard is cancelled and the controller returns t o the initial display. Any settings made so far remain val id.

9.2 Date and time settings

To access the date and time settings, go to Menu > Settings > Date and ti me

08.03.2013 05:05:06

Submenu “Date and time”. Select the desired function

and press the operating button.

Close Home

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Fixed value

8.03.2013

1 2 3 4 5 6 7 8 9

5:05:06

Function “Set date”

08.03.2013 05:05:06

..\ Select date (DD.MM.YYYY)

Ins Pos1 End Ok

Entry menu “Select date”.

The current date is shown. If it is incorrect, enter the correct date with the operating button.

Press the “Ok” button to confirm.

Close Ok Home

Function “Set time”

Fixed value

08.03.2013 05:05:06

..\ Set time (HH:MM:SS)

Ins Pos1 End Ok 1 2 3 4 5 6 7 8 9

Entry menu “Set time”.

The current time is shown. If it is incorrect, ent er the correct time with the operating button.

Press the “Ok” button to confirm.

Close Ok Home

There is no automatic switch to local light-saving times because this could lead to problems with data seeming to be missing or being overwritten in the data base.

Go back to the “Settings” menu with “Close” or to the initial view with “Home”.

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Fixed value

..\ Sprache, Language, Langue, Idioma, Lingua

Deutsch

English

Français

Español

Italiano

Fixed value

..\ Display brightness

9.3 Selecting the menu language of t he T4. 12 controller

The T4.12 chamber controller communicates via a comprehensible menu navigation in plain text in a selectable language.

To select the desired menu language, go to

Menu > Settings > Sprache, Language, Langue, Idioma, Lingua

08.03.2013 05:05:06

Submenu “Language”. Select the desired language

and press the operating button.

Close Home

Go back to the “Settings” menu with “Close” or to the initial view with “Home”.

9.4 Setting display brightness

To select the display brightness, go to Menu > Settings > Display bri ghtnes s

08.03.2013 05:05:06

Submenu “Display brightness”. Select the desired setting by turning the

operating button. Confirm by selecting “Save”.

Close Save Home

Go back to the “Settings” menu with “Close” or confirm the change with “Save”: The controller returns to the initial view.

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Fixed value

..\ Temperature unit

Celsius [°C]

Fahrenheit [°F]

Kelvin [K]

Fixed value

..\ Recording rate (seconds)

9.5 Changing the temperatur e uni t

To select the temperature unit, go to Menu > Settings > Temperature unit

08.03.2013 05:05:06

Submenu “Temperature unit”. Select the desired temperature unit”

and press the operating button.

Close Home

Go back to the “Settings” menu with “Close” or to the initial view with “Home”.

9.6 Defining the data recording rate

To define the recording rate for data storage on the SD card, go to Menu > Settings > Recording rate

08.03.2013 05:05:06

Ok 0 1 2 3 4 5 7 8 9 . – Del Ins

Close Ok Home

Go back to the “Settings” menu with “Close” or to the initial view with “Home”.

Note:

Entry menu “Recording rate”. The current recording rate is shown. Enter the

desired value with the operating button. Shortest possible value: 60 seconds. Press the “Ok” button to confirm.

After a period of 3 years, the controller starts overwriting the oldest values on the storage medium. This is independent of the selected storage interval and the actual operating time of the chamber. In any case, the data can be read out at any time using the function “Export to USB drive” (chap. 10.1) and stored externally.

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Fixed value

..\ Factory reset

Do not reset

Really reset

Fixed value

..\ Network settings

Show network settings

DHCP On/Off

MAC address

IP address

Subnet mask

Chamber name

Network name

Gateway

9.7 Factory reset

The “factory reset” function allows resetting the controller configuration to the factory settings.

Risk of data loss! When resetting to factory settings, all controller settings will be deleted. The

event list and stored measuring data are NOT affected.

To access the “Factory reset” function, go to Menu > Settings > Factory reset

08.03.2013 05:05:06

Submenu “Factory reset”. This a security question. Select the desired function

and press the operating button.

Close Home

Go back to the “Settings” menu with “Close” or to the initial view with “Home”.

9.8 Network configurat ion

The settings of this submenu are required for networking chambers with an Ethernet interface, e.g. to connect them with BINDER’s communication soft ware APT-COM™ 3 DataControlSystem.

You can display the chamber‘s IP address that has been assigned by your DHCP server or manually assign the IP address. All necessary configurations for networking the chamber are available in this menu.

To view and configure the network settings, go to Menu > Settings > Network settings

To configure the network settings, the DHCP state must be set to off. Otherwise, the DHCPserver would assign the network configuration.

If you try to configure the network settings with enabled DHCP state, the message “DHCP enabled!” is shown. Confirm with „Ok“ to return to the “Network settings” menu.

08.03.2013 05:05:06

Close Home

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Submenu “Network settings”. Turn the operating button to see additional menu

items.

Fixed value

..\ Network settings

DNS 1

DNS 2

Fixed value

..\ DHCP On/Off

DHCP : On

08.03.2013 05:05:06

Submenu “Network settings” (next page).

Close Home

Show network settings Overview of the entire network configurat i on DHCP On/Off

Switching on and off the DHCP state MAC address Displaying the MAC address IP address Entering the desired IP address Subnet mask Entering the subnet mask number Chamber name Entering the name of the CO2 incubator Network name Entering the network name Gateway E ntering the gateway number DNS 1 Entering the DNS 1 number DNS 2 Entering the DNS 2 number

Set the DHCP State on/off:

22.08.2012 05:05:06

Submenu “DHCP On/Off”. The current DHCP state is displayed. Press the

operating button to change it. Then the new DHCP state is displayed “DHCP : On” = DHCP state activated

“DHCP : Off” = DHCP state deactivated

Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.

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Close Home

You can only configure the following network settings if the DHCP state is set to “Off”.

Fixed value

..\ MAC address

00-04-A3-B1-00-B9

92.168.0.100

55.255.255.0

Display the MAC address

To identify the chamber in the Ethernet netwo rk y ou can display the chamber’s MAC address.

08.03.2013 05:05:06

Submenu “MAC address” (example value)

The MAC address is displayed.

Close Home

Go back to the “Network settings” menu with “Close ” or to the initial view with “Home”.

Enter the IP address:

Fixed value

08.03.2013 05:05:06

..\ IP address (n.n.n.n.)

Entry menu “IP address”

Ins Pos1 End Ok 0 2 3 4 5 6 7 8 9 .

(example value) Enter the desired IP address with the operating

button. Press the “Ok” button to confirm.

Close Ok Home

Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.

Enter the subnet mask:

Fixed value

08.03.2013 05:05:06

..\ Subnet mask (n.n.n.n.)

Entry menu “Subnet mask”

Pos1 End Ok 0 1 3 4 5 6 7 8 9 , -

(example value) Enter the desired net mask with the operating

button. Press the “Ok” button to confirm.

Close Ok Home

Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.

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..\ Chamber name

B_E6.1

Fixed value

..\ Network name

B_E6.1

Fixed value

..\ Gateway (n.n.n.n.)

92.168.0.1

Pos1 End Ok 0

2 3 4 5 6 7 8 9 ,

Home

Enter the chamber name:

Fixed value

End Ok _ : A B D E F G H I J K

08.03.2013 05:05:06

Entry menu “Chamber name”. Enter the desired chamber name with the operating

button. Press the “Ok” button to confirm.

Close Ok Home

Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.

Enter the network name:

08.03.2013 05:05:06

End Ok _ : A B D E F G H I J K

Entry menu “Network name”. Enter the desired network name with the operating

button. Press the “Ok” button to confirm.

Close Ok Home

Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.

Enter the default gateway:

08.03.2013 05:05:06

Entry menu “Gateway” (example value)

Enter the desired gateway with the operat ing button. Press the “Ok” button to confirm.

Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.

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..\ DNS 1 (n.n.n.n.)

92.168.0.1

Fixed value

..\ Show network settings

DHCP

Off

MAC address

00-04-A3-B1-00-B9

IP address

192.168.0.100

Net mask

255.255.255.0

Gateway

192.168.0.1

DNS1

192.168.0.1

DNS2

0.0.0.0

Chamber name

CB_E6.1

BIOS name

CB_E6.1

Enter the DNS 1 or DNS 2:

Fixed value

Ins Pos1 End Ok 0

08.03.2013 05:05:06

2 3 4 5 6 7 8 9 ,

Entry menu “DNS 1” or “DNS 2” (example value)

Enter the desired number with the operating button. Press the “Ok” button to confirm.

Close Ok Home

Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.

9.9 Display of the network configur at ion

To access the overview of the complete network configuration, go to

Menu > Settings > Network settings > Show network settings

08.03.2013 05:05:06

Overview of the network configuration (sample values)

Home

Go back to the “Network settings” menu with “Close” or to the initial view with “Home”.

CB (E6.1) 07/2017 Page 67/145

Fixed value

..\ RS 422 address

9.10 RS 422 address (with optional RS 422 interface)

For chambers equipped with the optional RS 422 interface, the RS 422 address serves to identify the chamber in a network and to establish communication with the optional BINDER communication software APT-COM™ 3 DataControlSystem. The factory default setting is “1”.

To enter the RS 422 chamber address, go to Menu > Settings > RS 422 address (optional)

08.03.2013 05:05:06

Pos1 End Ok 0 2 3 4 5 6 7 8 9

Close Ok Home

Entry menu “RS 422 address”. Enter the desired address (1 up to 254) with the

operating button. Press the “Ok” button to confirm.

Go back to the “Settings” menu with “Close” or to the initial view with “Home”.

9.11 Display and entry of the device configuration – for service purpose

Information about the chamber, such as chamber type, name, serial number, firmware version, etc. can be viewed under Menu > System information (chap. 6.7).

To access the device configuration menu, go t o Menu > Settings > Chamber configuration This menu is password protected and only intended for Service purpose.

CB (E6.1) 07/2017 Page 68/145

Fixed value

..\ Import/Export to USB drive

Export to USB drive

Import from USB drive

Home

Fixed value

..\ Export to USB drive

Chamber status

Measurement values

Event list

Configuration

Adjustment

Program parameters

System information

All

10. Data transfer via USB interface

The USB port is located in the instrument box. To access the submenus for data transfer, go to Menu > Import/Export

08.03.2013 05:05:06

Submenu “Import/Export to USB drive”. Select the desired type of communication v i a US B

port (data export or import) and press the operating button.

It is possible that some USB devices are not recognized due to compatibility issues. In this case, please use a USB storage device from a diff erent manufacturer.

10.1 Exporting data to USB drive

Insert the USB stick or the plug of your USB drive int o the USB port in the instrument box. To configure data export to USB media, go to Menu > Im port/ E xport > Expor t to USB drive

08.03.2013 05:05:06

Submenu “Export to USB drive”.

Close Home

Select the desired data type and press the operating button. Data is written to the connected media. Chamber status Actual chamber status, including operating mode, setpoints etc.

Measurement values Measured data Event list List of status information and errors (see chap. 12) Configuration (Service only) Adjustment Adjustment data Program parameters (Service only) System information All

CB (E6.1) 07/2017 Page 69/145

(Service only) All data

Fixed value

..\ Import from USB drive

Configuration

Firmware

If no USB device has been connected, the message “No US B device found” is displayed. Confirm the message with “OK” and insert the USB m edi um .

This symbol on the controller display in the initial view indicates that data are being transmitted via the USB port.

During a prolonged transfer, the message “USB data transfer in progress” is displayed. Confirm the message with “OK”.

Following data transfer, the message “USB data transfer completed” is displayed. Confirm the message with “OK”.

10.2 Importing data from USB drive

Insert the USB stick or the plug of your USB drive int o the USB port in the instrument box. To configure data import from USB media, go to Menu > Import/Export > Import from USB drive

08.03.2013 05:05:06

Submenu “Import from USB drive”. Select the desired data type

and press the operating button. Data is read from the connected media.

Close Home

Configuration Firmware

(Service only) (Service only)

If no USB device has been connected, the message “No US B device found” is displayed. Confirm the message with “OK” and insert the USB medium.

This symbol on the controller display in the initial view indicates that data are being transmitted via the USB port.

During a prolonged transfer, the message “USB data transfer in progress” is displayed. Confirm the message with “OK”.

Following data transfer, the message “USB data transfer completed” is displayed. Confirm the message with “OK”.

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Icon

Signification

Icon

Signification

Icon

Notifying message

Signification

Icon

Alarm message

Signification

11. Notifications and Alarms

11.1 Notifications overview

control deactivated. No O2

2/N2

Fixed value operation

concentration alarms will occur. (chamber with O

control)

Heating active

Key lock activated

Copying data via USB

Interior socket turned on (with option interior socket)

CO2 pressure alarm deactivated

O2 pressure alarm deactivated (chamber with O

control)

N2 pressure alarm deactivated (chamber with O

control)

Power return after power failure or shutdown of the chamber

DO NOT OPEN THE DOOR !

STERILIZATION FINISHED

STERILIZATION ABORTED

Hot air sterilization running

Hot air sterilization successful The inner chamber and parts inside can still be hot. Do not touch.

Hot air sterilization cancelled

The inner chamber and parts inside can still be hot. Do not touch.

11.2 Alarm messages overview

Safety controller overtemperature

Temp. range

CO2 range

O2 range

Overtemperature safety controller alarm (class 3.1): selected value of the safety controller exceeded.

Having reached the setpoint, the temperatur e deviates by more than the set tolerance range value (chap. 11.5.6) and longer than 10 min. from the setpoint or the temperature doesn’t reach the tolerance range within 3 hours from turning on the chamber or closing the d oor

Having reached the setpoint, the CO

concentration deviates by more than

the set tolerance range value (chap. 11.5.6) and longer than 10 min. from the setpoint or the CO

concentration doesn’t reach the tolerance range

within 3 hours from turning on the chamber or closin g the door Having reached the setpoint, the O

concentration deviates by more than

the set tolerance range value (chap. 11.5.6) and longer than 10 min. from the setpoint or the O

concentration doesn’t reach the tolerance range

within 3 hours from turning on the chamber or closin g the door (chamber with O

control)

CB (E6.1) 07/2017 Page 71/145

Icon

Alarm message

Signification

Door open

Door is open for more than the set alarm delay time (chap. 11.5.5, factory setting: 1 minute). Close the door.

Low pressure CO2 Low CO2 outlet pressure (< 0.3 bar)

Low pressure CO2 Low O2 outlet pressure (< 0.3 bar) (chamber with O2 control)

Low pressure N2 Low N2 outlet pressure (< 0.3 bar) (chamber with O2 control)

CO2 sensor defective

O2 sensor defective

Inform service adjust CO2 sensor

sensor defective. Contact BINDER Service.

sensor defective. Contact BINDER Servi ce.

sensor adjustment required. Contact B INDER Service.

You can activate / deactivate the buzzer in the “Alarms” submenu (chap. 11.5.3). With an activated buzzer there is an audible alert with an alarm. You can reset it in the “Alarms”

submenu for alarm acknowledgement pressing the “Reset” button (chap. 11.4). The alarm symbol will only disappear when the cause of the alarm has been remedied.

The zero-voltage relay alarm contact (chap. 11.6) is activated in case of the alarm messages “door open” and deviations of temperature and gas concentrations) as well as in case of a power failure and turning off the chamber at its main power switch.

For appropriate actions in the event of an alarm, please refer to chap. 21 “Troubleshooting”.

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11.3 Alarm status

An alarm message can appear in 3 different states:

“Set”

• Active alarm.

• The corresponding alarm icon is displayed in the initial view The buzzer sounds (if activated).

• The “Info” button in the initial view leads to the “Alarms” submenu for alarm acknowledgement.

• Press the “Reset” button in the “Alarms” submenu for alarm acknowledgement to mute the buzzer and

confirm the alarm.

“Acknowledged”

• Active alarm.

• The alarm was acknowledged. The buzzer is off.

• The alarm cause is still valid. Therefore, the alarm icon remains displayed in the initial view.

• The alarm message figures in the list of active alarms.

“Cleared”

• The alarm cause has disappeared.

• The alarm icon is not displayed any longer

• The alarm message has disappeared from the list of active alarms.

• The alarm message remains in the “Event list” for information.

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Fixed value

Setpoint

Actual value

37.0

39.2

5.0

25.0

Info

Alarm acknowledgement

../Home/Alarms

Safety controller overtemperature

11.4 Confirming an active “set” alarm

08.03.2013 05:05:06

Temperature [°C]

Initial view with overtemperature safety c ontroller

CO2 [Vol.-%]

O2 [Vol.-%]

alarm. The buzzer sounds (if not deactivated previously).

Press the “Info” button.

User

Close Reset Home

Press the “Reset” button to confirm the alarm. If there is another active “set” alarm, you can also confirm it with the “Reset” button. After confirming all active “set” alarms, the buzzer is off, and the initial view is displayed.

As long as an alarm is still active, i.e. as long as the alarm cause is still valid, the alarm icon continues to be displayed in the initial view.

08.03.2013 05:05:06

Submenu “Alarms” for alarm acknowledgement.

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Fixed value

..\ Alarm

Active alarms

History

Buzzer test

Buzzer On/Off

Alarms On/Off

Door alarm delay

Tolerance ranges

Fixed value

..\ Active alarms

Safety controller overtemperature

11.5 Alarm configuration and overview

To access the alarm lists and configuration menu, go t o Menu > Alarms

08.03.2013 05:05:06

Submenu “Alarm”. Select the desired function

and press the operating button.

Close Home

Active Alarms List of the alarms with status “set” or “acknowledged”. History List of all alarms (status “set” or “acknowledged” or “cleared”) Buzzer test Testing the alarm buzzer, chap. 11.5.3 Buzzer On/Off Activating / deacti vating the alarm buzzer, chap. 11.5.3

Alarms On/Off

Activating / deactivating the alarm functions. Off: Alarm buzzer off, no alarm icons displayed.

Door alarm delay Entering the delay time of the door alarm Tolerance ranges

Defining the tolerance ranges and alarm delay times for the individual parameters

11.5.1 List of active alarms

To access the overview list of active alarms, go t o M en u > Al arms > Active alarms

08.03.2013 05:05:06

Submenu “Active alarms“. All active alarms with status “set”

are listed.

or “acknowledged”,

Close Home

If no alarm is active (“set” or “acknowledged”), no message will be displ ayed in this window.

CB (E6.1) 07/2017 Page 75/145

Fixed value

..\ History

08.03.2013 : Messages of indicated day

14:39:48

Alarm set Safety controller overtemperature

15:03:22

Alarm cleared Safety controller overtemperature

8.03.2013

1 2 3 4 5 6 7 8 9

Fixed value

..\ Event list

08.03.2013 : Messages of indicated day