Agilent Technologies Separation of Oxindole Alkaloids Technical data

Separation of Oxindole Alkaloids

Application

Agrichemical

Robert Ricker

Oxindole Alkaloids are a major component (in addition to triterpenes) of root-bark extracts from Uncaria tomentosa. These extracts have been used as folk medicine, and more recently, to treat cancer, viral infections, intestinal and epidermal disorders, and arthritis. Previous HPLC methods have given unsatisfactory results. Due to the potential therapeutic importance of these compounds, a new, rugged HPLC method was developed.

Highlights

1. Spectiophyllin

2. Uncarin F

3. Mitraphyllin

4. Pteropodin

5. Rhynchophyllin

6. Isorrhynchophyllin

7. Isopteropodin

Courtesy of Dr. H. Stuppner, Inst. Pharmacognosy, Univ. of Innsbruck Austria

• High resolution and good peak shape

for a number of oxindole alkaloids on a Rx-C18 at neutral pH.

• The good retention of the alkaloids

under these conditions separates them from other UV absorbing compounds in the sample.

• Use additional caution when running

silica-based columns at pH 6 and above since silica degrades much more quickly at higher pH.

Conditions: Column: ZORBAX RX-C18, 4.6 x 250 mm (Agilent Part No. 880967-902) Mobile Phase:

Gradient: 45-70% B in 35 min. Injection: 10µL; UV: 245 nm; Flow: 1.0 mL / min.; 15°C

A 10 mM Phosphate Buffer pH 6.6 B (1:1) MeOH : ACN

Robert Ricker is an application chemist

based at Agilent Technologies, Wilmington,

Delaware.

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Printed in the USA

April 25, 2002

5988-6290EN