Agilent Technologies Quaternary Pump 1100 User Manual

XCALI-97190 Revision B July 2007

Agilent®1100 Series

LC Devices

Connecting the Hardware and Triggering Data
Acquisition

© 2007 Thermo Fisher Scientific Inc. All rights reserved.

Agilent® is a registered trademark of Agilent Technologies Inc. Hewlett-Packard® is a registered trademark
of Hewlett-Packard Company. LCQ™, LTQ™, LXQ™, MSQ™, TSQ™, and Xcalibur™ are trademarks of
Thermo Fisher Scientific Inc.

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the
product operation. This document is copyright protected and any reproduction of the whole or any part of this
document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.

The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.

Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error­free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might
result from any use of this document, even if the information in the document is followed properly.

This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This
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Sale shall govern all conflicting information between the two documents.

Release history: First release February 2007, second release July 2007

For Research Use Only. Not regulated for medical or veterinary diagnostic use by U.S. Federal Drug
Administration or other competent authorities.

Contents

C

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .v

About This Guide. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v

Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v

Safety and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v

Safety Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .vi

Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .vi

Chapter 1 Agilent Modules Supported by LC Devices Versions 2.0.2 and 2.1.0 . . . . . . . . . . .1

Chapter 2 Connecting the Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3

Ethernet Communication Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Installing a Network Card in an Agilent Module. . . . . . . . . . . . . . . . . . . . . . . . . 4

Connecting the Ethernet Communication Cables . . . . . . . . . . . . . . . . . . . . . . . . 7

Installing the External Contact Interface Board . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Connecting the Trigger Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Chapter 3 Configuring the Autosampler for Contact Closure . . . . . . . . . . . . . . . . . . . . . . . . .11

Chapter 4 Triggering Data Acquisition with the Autosampler . . . . . . . . . . . . . . . . . . . . . . . .13

Chapter 5 Turning Off the Solvent Tracking Feature. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15

Appendix A Upgrading an HP 1100 Series LC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19

Thermo Scientific Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition iii

Preface

About This Guide

This guide describes how to hardwire an Agilent® 1100 Series LC to a Thermo Scientific
mass spectrometer and the data system computer, configure an Agilent autosampler for
contact closure from within the Xcalibur Instrument Configuration program, and trigger data
collection with an Agilent autosampler.

This guide also describes how to upgrade a Hewlett-Packard® 1100 Series LC to an
Agilent 1100 Series LC with Ethernet communication.

P

Related Documentation

In addition to this guide, Thermo Fisher Scientific provides Help available from within the
Xcalibur software.

Safety and Special Notices

Make sure you follow the precautionary statements presented in this guide. The safety and
other special notices appear in boxes.

Safety and special notices include the following:

CAUTION Highlights hazards to humans, property, or the environment. Each CAUTION
notice is accompanied by an appropriate CAUTION symbol.

CAUTION Highlights electric shock related hazards to human beings. Each electric shock
notice is accompanied by the international High Voltage symbol.

IMPORTANT Highlights information necessary to prevent damage to software, loss of
data, or invalid test results; or might contain information that is critical for optimal
performance of the system.

Thermo Scientific Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition v

Preface

Safety Precautions

Note Highlights information of general interest.

Tip Helpful information that can make a task easier.

To connect the Agilent 1100 Series LC stack to a Thermo Scientific mass spectrometer, you
must install an external contact board in one of the Agilent modules and a LAN interface
board in a second Agilent module. To install a board, you must remove the cover from the
optional board slot. Figure 2 on page 6 shows the location of the slot on the back panel of the
detector.

Before you remove the slot cover from an Agilent 1100 Series module, do the following:

• Turn the power switch to the Off position and unplug the power cable.

Contacting Us

CAUTION To avoid an electric shock, always turn off an Agilent module and unplug

it from line power before you remove its cover.

• Refer to the Reference Manual for the module for additional safety information and for
information on preventing ESD damage caused by an electrical discharge.

CAUTION To prevent damage to an instrument, always use ESD protection when
handling electronic boards and components.

There are several ways to contact Thermo Fisher Scientific.

Y To contact Technical Support

Phone [phone]
Fax [phone]
E-mail [e-mail]
Knowledge base www.thermokb.com

Find software updates and utilities to download at www.mssupport.thermo.com.

vi Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition Thermo Scientific

Y To contact Customer Service for ordering information

Phone [phone]
Fax [phone]
Web si te www.thermo.com/finnigan

Y To suggest changes to documentation or to Help

• Fill out a reader survey online at www.thermo.com/lcms-techpubs.

• Send an e-mail message to the Technical Publications Editor at

[e-mail].

Preface

Thermo Scientific Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition vii

Agilent Modules Supported by LC Devices Versions 2.0.2 and 2.1.0

Ta bl e 1 lists the model number and firmware versions for the Agilent 1100 Series LC

modules that are supported by the Xcalibur 2.0 and higher data system.

Table 1. Xcalibur 2.0 supported firmware versions for the Agilent 1100 Series LC

Module Model Number Firmware Version

Binary pump G1312A A.06.01 (012)

Quaternary pump G1311A A.06.01 (012)

1

Autosampler G1313A A.06.01 (012)

Thermostatted autosampler G1329A A.06.01 (012)

Well-plate autosampler G1367A A.06.01 (012)

Thermostatted column compartment G1316A A.06.01 (012)

Variable wavelength detector G1314A A.06.01 (012)

Diode-array detector G1315A A.06.01 (012)

Multiple wavelength detector

*

Only works with version B of the Control Module.

Y To check the firmware version of an Agilent 1100 module

1. Make sure that the Agilent 1100 Series modules are connected by CAN communication
cables, that all the modules are turned on, and that the Agilent 1100 Control Module is
connected.

Refer to the Agilent 1100 Series LC reference manuals for instructions on how to connect
the CAN cables.

2. Press the Esc key on the Control Module until System appears in the upper-left corner of
the LCD display.

*

G1365B A.06.01 (012)

3. Press the F4 key on the Control Module to access the Records LCD display.

Thermo Scientific Agilent 1100 Series LC Devices Connecting the Hardware and Triggering Data Acquisition 1