Agilent Technologies Polystyrene Standards service manual
Polystyrene Standards with Less
Solvent
Agilent PLgel 5 μm MiniMIX-D
Columns
Technical Overview
Introduction
Agilent narrow bore GPC columns offer high performance, excellent solvent
compatibility and mechanical stability. These columns can be used with
conventional GPC equipment and provide high performance, comparable to our
conventional ID columns, the benefi t of ~ 70% reduction in solvent consumption,
increased operator safety and reduced solvent disposal costs.
To maintain the same linear velocity
through the column, the volumetric fl ow
rate must be reduced to 0.3 mL/min,
in line with the column cross sectional
Sample loadings are scaled down in
line with the reduced column volume,
and system dead volume minimized to
avoid excessive band broadening.
area. This provides signifi cantly lower
solvent consumption.
Column: 3 x PLgel 5 μm MIXED-D, 300 x 7.5 mm
(part number PL1110-6504)
Eluent: THF
Flow Rate: 1.0 mL/min
Detection: RI
PLgel 5 μm MiniMIX-D columns can
be calibrated using EasiCal™ PS-2 preprepared polystyrene calibrants.
3
2
4
1
8
7
6
8 20min
Figure 1. PLgel 5 μm MiniMIX-D column calibration using EasiCal PS-2 calibrants
5
9
Peak Identifi cation (Mw)
1. 380,000
2. 96,000
3. 22,000
4. 5,050
5. 1,320
6. 156,000
7. 49,900
8. 11,600
9. 2,950
10. 580
10
www.agilent.com/chem
This information is subject to change without notice.
© Agilent Technologies, Inc. 2010
Published in UK, September 21, 2010
SI-01827
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