3M Zeta Plus User Manual

3M Separation and Purication Sciences

™

3M

Zeta Plus

Depth Filters

High Performance, Scalable,
Single-Use System

™

3M™ Zeta Plus™ Encapsulated System

The System of Choice for Single-Use Depth Filtration

High Performance
Filter Media

The 3M™ Zeta Plus™ Encapsulated
System utilizes the high performing
Zeta Plus depth lter series
media, including the single and
dual layer.

• Positive charge is capable of

reducing negatively charged
DNA, endotoxins and other host
cell proteins

™

• The 3M

media enhances the contaminant

holding capacity of the lter media.

This allows for larger particles to
be trapped in the upstream zone

of the more open lter media and

smaller particles to be trapped in
the downstream zone, reducing
premature plugging and helping
extend service life of the media.

• Can be used for post fermentation

cell culture clarication or

downstream impurity removal

• Can be employed independently or

in conjunction with centrifugation or

tangential ow ltration (TFF)

Zeta Plus™ dual layer

3M™ Zeta Plus™ Depth Filter Quick Start Guide

Application Stage / Product

First Stage Second Stage

Two Stage
Operations

Single Stage
Operations

Post Centrifuge
Operations

05SP01
10SP02

60SP02

90ZB08
90SP08
60ZB05
60SP05

90ZB08
90SP08
60ZB05
60SP05

Media Series

SP Media LA Media ZB Media

Widest Range Cleanest Highly Charged

SP has the widest nominal
pore size range relative to
other 3M Zeta Plus media

oerings, including a

greater number of grades
as well as grades with
larger nominal pore sizes
than LA or ZB media.

LA is the cleanest
3M Zeta Plus media family
offered. 3M

series low aluminum (LA)

filter media are designed
to provide low levels of
extractables, especially
aluminum.

™

Zeta Plus™ LA

ZB media offers a higher
charge level than SP or LA
media, and offers single
layer and dual layer grades
with a smaller nominal
pore size than either the
SP media family or the LA
media family.

• Activated carbon and lipid removal

media also available

2 | 3M™ Zeta Plus™ Single-Use Depth Filtration Product Portfolio

Sizing Guide

Pore Size Options: 3M™ Zeta Plus™ SP, LA and ZB Media

Media Family

Grades SP ZB LA Application

5 X

10 X

30 X X X

50 X

60 X X X

90 X X X

120 X

For reference only. Retention ratings may vary depending on application.

Primary

Secondary

Centrate

Features & Benets

Capsule/Manifold Design

Translucent plastic shell

(standard capsules,
polycarbonate shells)

Fully encapsulated shell
around solid core

Self guiding locking
mechanism

Lenticular style capsule
design

3M™ Zeta Plus™ Capsules:

Encapsulated Standard Capsule

with Polycarbonate Shells

• Easy detection of the liquid

level inside, providing real time
monitoring of the filtration
process.

• Eliminates the need for a

stainless steel housing and the
cleaning step after filtration.

• Fast and reliable capsule-to-

capsule connectivity.

• Consistency between

single-use and conventional
depth filtration.

3M™ Zeta Plus™ Capsules: Encapsulated

Capsule with Alkaline Resistant*

Polyphenylene Oxide/Polystyrene

3M™ Zeta Plus™ Filter Media

Model# 16EZB

• Both single and dual layer Zeta Plus filter

media are available.

• Excellent performance in throughput and

filtration efficiency with proper media
selection and sizing.

3M™ Zeta Plus™ Capsule Family

* Based on testing with 1M NaOH and 5% NaClO (Bleach).

• The 3M

a single use depth filtration system

• The complete system is comprised of a

holder, two manifolds and the desired number
of capsules

• The polycarbonate capsules feature a

translucent shell that allows for easy fluid
level observation

• A self-guiding locking mechanism ensures fast

and reliable connections between capsules

Self Guiding Locking
Mechanism Enables
Fast and Reliable
Capsule-To-Capsule
Connection

™

Zeta Plus™ Encapsulated System is

3M™ Zeta Plus™ Single-Use Depth Filtration Product Portfolio | 3

3M™ Zeta Plus™ Encapsulated System

The System of Choice for Single-Use Depth Filtration

Ergonomically Designed
Large Filter Holders

Traditional depth ltration systems utilize lenticular style
cartridge lters and a vertical ltration ow path to allow
easy access to process liquids and ecient utilization of
lter media. However, stacking cartridges from bottom

to top can be cumbersome, and dismantling the spent
cartridges is often labor intensive.

Features & Benets

Ergonomically Designed Holder System

3M™ Encapsulated System

Holder, Large (Model
#16EZB): holder is pivoted

between horizontal and
vertical positions

Vertical flow path

• Enables loading and unloading at

waist height.

• Central inlets and outlets minimize

fluid spills during post use handing.

• Holder and capsule design

allows the combination of multiple
3M Zeta Plus media types or even
multiple 3M filtration products
in a single holder.

• Reduced footprint during operation.

4 | 3M™ Zeta Plus™ Single-Use Depth Filtration Product Portfolio

Recognizing the need for a depth ltration system that is fast, easy and clean, 3M designed lter holders
(Model# 16EZB) that can be pivoted between the horizontal position for loading and unloading the
capsules and manifolds, and the vertical position for ltration. Allowing loading and unloading at waist
height eliminates the need for operators to lift capsules above their heads and reduces the risk of uid
spills when handling spent capsules. The use of the vertical ow path allows for full media utilization and
a small system footprint during ltration.

3M™ Encapsulated System Holders, Small
(Model# 16EZA)

The small holder is available for laboratory and pilot scale-up

studies, in addition to low volume production ltration. The

1-high holder can accommodate from one to four 0.23 m²

capsules, or one 1.6 m² (dual layer) or 2.5 m² (single layer)

capsule. The 2-high holder can accommodate up to two 1.6 m²

(dual layer) or 2.5 m² (single layer) capsules. The 3 high holder
can accommodate up to three 1.6 m² (dual layer) or 2.5 m²
(single layer) capsules. Either single stage or two-stage depth
ltration can be performed within the same holder. The 1-high

small holder has a built-in torque limiter that will signal the
operator when the holder assembly is properly sealed. All
small holders have been designed to be fully autoclavable for
applications where that may be required.

3M™ Encapsulated System Holders, Large
(Model# 16EZB)

The large holder can accommodate up seven 1.6 m² (dual layer)
or 2.5 m² (single layer) capsules. This holder is best suited for
use in small to large production scale purication processes.

However, this holder can also accommodate a single 1.6 m²

(dual layer) or 2.5 m² (single layer) capsule should choose to use

it for scale up studies.

Two Stage Operations

For two stage purication operations a

second pair of manifolds is required between
each stage of multistage operations.
Manifold and capsule materials should
always be the same.

Figure 1. 3M™ Zeta Plus™ Encapsulated System

3M™ Zeta Plus™ Single-Use Depth Filtration Product Portfolio | 5