3M Separation and Purification Sciences
3M™ Zeta Plus™
Depth Filters
High Performance, Scalable,
Single-Use System
3M™ Zeta Plus™ Encapsulated System
The System of Choice for Single-Use Depth Filtration
High Performance
Filter Media
The 3M™ Zeta Plus™ Encapsulated System utilizes the high performing
Zeta Plus depth filter series media, including the single and dual layer.
• Positive charge is capable of reducing negatively charged DNA, endotoxins and other host cell proteins
•The 3M™ Zeta Plus™ dual layer media enhances the contaminant holding capacity of the filter media.
This allows for larger particles to be trapped in the upstream zone of the more open filter media and smaller particles to be trapped in the downstream zone, reducing premature plugging and helping extend service life of the media.
• Can be used for post fermentation cell culture clarification or downstream impurity removal
• Can be employed independently or in conjunction with centrifugation or tangential flow filtration (TFF)
• Activated carbon and lipid removal media also available
3M™ Zeta Plus™ Depth Filter Quick Start Guide
Application |
Stage / Product |
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First Stage |
Second Stage |
Two Stage |
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90ZB08 |
05SP01 |
90SP08 |
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Operations |
10SP02 |
60ZB05 |
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60SP05 |
Single Stage |
60SP02 |
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Operations |
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Post Centrifuge |
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90ZB08 |
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90SP08 |
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Operations |
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60ZB05 |
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60SP05 |
Media Series
SP Media |
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LA Media |
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ZB Media |
Widest Range |
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Cleanest |
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Highly Charged |
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SP has the widest nominal |
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LA is the cleanest |
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ZB media offers a higher |
pore size range relative to |
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3M Zeta Plus media family |
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charge level than SP or LA |
other 3M Zeta Plus media |
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offered. 3M™ Zeta Plus™ LA |
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media, and offers single |
offerings, including a |
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series low aluminum (LA) |
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layer and dual layer grades |
greater number of grades |
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filter media are designed |
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with a smaller nominal |
as well as grades with |
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to provide low levels of |
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pore size than either the |
larger nominal pore sizes |
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extractables, especially |
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SP media family or the LA |
than LA or ZB media. |
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aluminum. |
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media family. |
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Sizing Guide
Pore Size Options: 3M™ Zeta Plus™ SP, LA and ZB Media
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Media Family |
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Grades |
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SP |
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ZB |
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LA |
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Application |
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5 |
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X |
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10 |
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X |
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Primary |
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30 |
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X |
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X |
X |
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50 |
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X |
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Secondary |
60 |
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X |
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X |
X |
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90 |
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X |
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X |
X |
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Centrate |
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120 |
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X |
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For reference only. Retention ratings may vary depending on application.
2 | 3M™ Zeta Plus™ Single-Use Depth Filtration Product Portfolio
Features & Benefits
Capsule/Manifold Design |
3M™ Zeta Plus™ Filter Media |
Translucent plastic shell
(standard capsules, polycarbonate shells)
Fully encapsulated shell around solid core
Self guiding locking mechanism
Lenticular style capsule design
• Easy detection of the liquid level inside, providing real time monitoring of the filtration process.
• Eliminates the need for a stainless steel housing and the cleaning step after filtration.
• Fast and reliable capsule-to- capsule connectivity.
•Consistency between single-use and conventional depth filtration.
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Model# 16EZB |
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• Both single and dual layer Zeta Plus filter |
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media are available. |
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3M™ Zeta Plus™ Capsules: |
3M™ Zeta Plus™ Capsules: Encapsulated |
• Excellent performance in throughput and |
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filtration efficiency with proper media |
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Encapsulated Standard Capsule |
Capsule with Alkaline Resistant* |
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with Polycarbonate Shells |
Polyphenylene Oxide/Polystyrene |
selection and sizing. |
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• The 3M™ Zeta Plus™ Encapsulated System is |
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a single use depth filtration system |
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• The complete system is comprised of a |
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holder, two manifolds and the desired number |
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of capsules |
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• The polycarbonate capsules feature a |
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3M™ Zeta Plus™ Capsule Family |
translucent shell that allows for easy fluid |
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level observation |
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• A self-guiding locking mechanism ensures fast |
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and reliable connections between capsules |
Self Guiding Locking
Mechanism Enables
Fast and Reliable
Capsule-To-Capsule
Connection
* Based on testing with 1M NaOH and 5% NaClO (Bleach).
3M™ Zeta Plus™ Single-Use Depth Filtration Product Portfolio | 3
3M™ Zeta Plus™ Encapsulated System
The System of Choice for Single-Use Depth Filtration
Ergonomically Designed
Large Filter Holders
Traditional depth filtration systems utilize lenticular style cartridge filters and a vertical filtration flow path to allow easy access to process liquids and efficient utilization of filter media. However, stacking cartridges from bottom to top can be cumbersome, and dismantling the spent cartridges is often labor intensive.
Features & Benefits
Ergonomically Designed Holder System
3M™ Encapsulated System
Holder, Large (Model #16EZB): holder is pivoted between horizontal and vertical positions
Vertical flow path
• Enables loading and unloading at waist height.
• Central inlets and outlets minimize fluid spills during post use handing.
• Holder and capsule design
allows the combination of multiple 3M Zeta Plus media types or even multiple 3M filtration products
in a single holder.
• Reduced footprint during operation.
4 | 3M™ Zeta Plus™ Single-Use Depth Filtration Product Portfolio
Recognizing the need for a depth filtration system that is fast, easy and clean, 3M designed filter holders (Model# 16EZB) that can be pivoted between the horizontal position for loading and unloading the capsules and manifolds, and the vertical position for filtration. Allowing loading and unloading at waist height eliminates the need for operators to lift capsules above their heads and reduces the risk of fluid spills when handling spent capsules. The use of the vertical flow path allows for full media utilization and a small system footprint during filtration.
3M™ Encapsulated System Holders, Small (Model# 16EZA)
The small holder is available for laboratory and pilot scale-up studies, in addition to low volume production filtration. The
1-high holder can accommodate from one to four 0.23 m² capsules, or one 1.6 m² (dual layer) or 2.5 m² (single layer) capsule. The 2-high holder can accommodate up to two 1.6 m²
(dual layer) or 2.5 m² (single layer) capsules. The 3 high holder can accommodate up to three 1.6 m² (dual layer) or 2.5 m² (single layer) capsules. Either single stage or two-stage depth filtration can be performed within the same holder. The 1-high small holder has a built-in torque limiter that will signal the operator when the holder assembly is properly sealed. All small holders have been designed to be fully autoclavable for applications where that may be required.
3M™ Encapsulated System Holders, Large (Model# 16EZB)
The large holder can accommodate up seven 1.6 m² (dual layer) or 2.5 m² (single layer) capsules. This holder is best suited for use in small to large production scale purification processes.
However, this holder can also accommodate a single 1.6 m²
(dual layer) or 2.5 m² (single layer) capsule should choose to use it for scale up studies.
Two Stage Operations
For two stage purification operations a second pair of manifolds is required between each stage of multistage operations. Manifold and capsule materials should always be the same.
Figure 1. 3M™ Zeta Plus™ Encapsulated System
3M™ Zeta Plus™ Single-Use Depth Filtration Product Portfolio | 5