3M Zeta Plus User Manual

3M Separation and Purification Sciences

3M™ Zeta Plus™

Depth Filters

High Performance, Scalable,

Single-Use System

3M™ Zeta Plus™ Encapsulated System

The System of Choice for Single-Use Depth Filtration

High Performance

Filter Media

The 3M™ Zeta Plus™ Encapsulated System utilizes the high performing

Zeta Plus depth filter series media, including the single and dual layer.

•  Positive charge is capable of reducing negatively charged DNA, endotoxins and other host cell proteins

•The 3M™ Zeta Plus™ dual layer media enhances the contaminant holding capacity of the filter media.

This allows for larger particles to be trapped in the upstream zone of the more open filter media and smaller particles to be trapped in the downstream zone, reducing premature plugging and helping extend service life of the media.

•  Can be used for post fermentation cell culture clarification or downstream impurity removal

•  Can be employed independently or in conjunction with centrifugation or tangential flow filtration (TFF)

•  Activated carbon and lipid removal media also available

3M™ Zeta Plus™ Depth Filter Quick Start Guide

Application	Stage / Product
	First Stage	Second Stage
Two Stage		90ZB08
	05SP01	90SP08
Operations	10SP02	60ZB05
		60SP05
Single Stage	60SP02
Operations
Post Centrifuge		90ZB08
		90SP08
Operations		60ZB05
		60SP05

Media Series

SP Media		LA Media		ZB Media
Widest Range		Cleanest		Highly Charged
SP has the widest nominal		LA is the cleanest		ZB media offers a higher
pore size range relative to		3M Zeta Plus media family		charge level than SP or LA
other 3M Zeta Plus media		offered. 3M™ Zeta Plus™ LA		media, and offers single
offerings, including a		series low aluminum (LA)		layer and dual layer grades
greater number of grades		filter media are designed		with a smaller nominal
as well as grades with		to provide low levels of		pore size than either the
larger nominal pore sizes		extractables, especially		SP media family or the LA
than LA or ZB media.		aluminum.		media family.

Sizing Guide

Pore Size Options: 3M™ Zeta Plus™ SP, LA and ZB Media

				Media Family
Grades		SP		ZB		LA		Application
5		X
10		X						Primary
30		X		X		X
50						X		Secondary
60		X		X		X
90		X		X		X		Centrate
120				X

For reference only. Retention ratings may vary depending on application.

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Features & Benefits

Capsule/Manifold Design

3M™ Zeta Plus™ Filter Media

Translucent plastic shell

(standard capsules, polycarbonate shells)

Fully encapsulated shell around solid core

Self guiding locking mechanism

Lenticular style capsule design

•  Easy detection of the liquid level inside, providing real time monitoring of the filtration process.

•  Eliminates the need for a stainless steel housing and the cleaning step after filtration.

•  Fast and reliable capsule-to- capsule connectivity.

•Consistency between single-use and conventional depth filtration.

				Model# 16EZB
				•  Both single and dual layer Zeta Plus filter
				media are available.
	3M™ Zeta Plus™ Capsules:	3M™ Zeta Plus™ Capsules: Encapsulated		•  Excellent performance in throughput and
				filtration efficiency with proper media
Encapsulated Standard Capsule		Capsule with Alkaline Resistant*
	with Polycarbonate Shells	Polyphenylene Oxide/Polystyrene		selection and sizing.
				• The 3M™ Zeta Plus™ Encapsulated System is
				a single use depth filtration system
				•  The complete system is comprised of a
				holder, two manifolds and the desired number
				of capsules
				•  The polycarbonate capsules feature a
	3M™ Zeta Plus™ Capsule Family			translucent shell that allows for easy fluid
				level observation
				•  A self-guiding locking mechanism ensures fast
				and reliable connections between capsules

Self Guiding Locking

Mechanism Enables

Fast and Reliable

Capsule-To-Capsule

Connection

* Based on testing with 1M NaOH and 5% NaClO (Bleach).

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3M™ Zeta Plus™ Encapsulated System

The System of Choice for Single-Use Depth Filtration

Ergonomically Designed

Large Filter Holders

Traditional depth filtration systems utilize lenticular style cartridge filters and a vertical filtration flow path to allow easy access to process liquids and efficient utilization of filter media. However, stacking cartridges from bottom to top can be cumbersome, and dismantling the spent cartridges is often labor intensive.

Features & Benefits

Ergonomically Designed Holder System

3M™ Encapsulated System

Holder, Large (Model #16EZB): holder is pivoted between horizontal and vertical positions

Vertical flow path

•  Enables loading and unloading at waist height.

•  Central inlets and outlets minimize fluid spills during post use handing.

•  Holder and capsule design

allows the combination of multiple 3M Zeta Plus media types or even multiple 3M filtration products

in a single holder.

•  Reduced footprint during operation.

4 | 3M™ Zeta Plus™ Single-Use Depth Filtration Product Portfolio

Recognizing the need for a depth filtration system that is fast, easy and clean, 3M designed filter holders (Model# 16EZB) that can be pivoted between the horizontal position for loading and unloading the capsules and manifolds, and the vertical position for filtration. Allowing loading and unloading at waist height eliminates the need for operators to lift capsules above their heads and reduces the risk of fluid spills when handling spent capsules. The use of the vertical flow path allows for full media utilization and a small system footprint during filtration.

3M™ Encapsulated System Holders, Small (Model# 16EZA)

The small holder is available for laboratory and pilot scale-up studies, in addition to low volume production filtration. The

1-high holder can accommodate from one to four 0.23 m² capsules, or one 1.6 m² (dual layer) or 2.5 m² (single layer) capsule. The 2-high holder can accommodate up to two 1.6 m²

(dual layer) or 2.5 m² (single layer) capsules. The 3 high holder can accommodate up to three 1.6 m² (dual layer) or 2.5 m² (single layer) capsules. Either single stage or two-stage depth filtration can be performed within the same holder. The 1-high small holder has a built-in torque limiter that will signal the operator when the holder assembly is properly sealed. All small holders have been designed to be fully autoclavable for applications where that may be required.

3M™ Encapsulated System Holders, Large (Model# 16EZB)

The large holder can accommodate up seven 1.6 m² (dual layer) or 2.5 m² (single layer) capsules. This holder is best suited for use in small to large production scale purification processes.

However, this holder can also accommodate a single 1.6 m²

(dual layer) or 2.5 m² (single layer) capsule should choose to use it for scale up studies.

Two Stage Operations

For two stage purification operations a second pair of manifolds is required between each stage of multistage operations. Manifold and capsule materials should always be the same.

Figure 1. 3M™ Zeta Plus™ Encapsulated System

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