Xylem 6100-VIS User Manual

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photoLab

Functional description

6100 VIS

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Spectrophotometer

(identical to spectroFlex 6100)

photoLab®6100 VIS

Accuracy when going to

press

The use of advanced technology and the high quality standard of our instruments are the result of continuous development. This may result in differences between this operating manual and your instrument. Also, we cannot guarantee that there are absolutely no errors in this manual. Therefore, we are sure you will understand that we cannot accept any legal claims resulting from the data, figures or descriptions.

Note

The latest version of the present operating manual is available on the Internet under http://www.WTW.com.

Reprinting - even as excerpts - is only allowed with the explicit written authorization of WTW GmbH. Printed in Germany.

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photoLab®6100 VIS Contents

photoLab®6100 VIS - Contents

1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

1.1 Overview of the instrument . . . . . . . . . . . . . . . . . . . . . . . 7

1.2 Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

1.3 Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2 Safety instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

2.1 Target group and user qualification . . . . . . . . . . . . . . . . 11

2.2 Authorized use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

2.3 General safety instructions . . . . . . . . . . . . . . . . . . . . . . . 12

2.4 Handling of hazardous substances . . . . . . . . . . . . . . . . 13

3 Commissioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.1 Scope of delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.2 Initial commissioning . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.2.1 Inserting the buffer batteries . . . . . . . . . . . . . . . 16

3.2.2 Connecting the power supply . . . . . . . . . . . . . . 17

3.2.3 Switching on the photometer for the first time . . 18

3.2.4 Setting the language . . . . . . . . . . . . . . . . . . . . . 18

3.2.5 Setting the date and time . . . . . . . . . . . . . . . . . 19

3.3 Connecting optional accessories . . . . . . . . . . . . . . . . . . 20

3.3.1 Communication interfaces . . . . . . . . . . . . . . . . . 20

3.3.2 PC/printer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

3.3.3 USB memory device . . . . . . . . . . . . . . . . . . . . . 22

3.3.4 PC keyboard . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

3.3.5 Barcode reader . . . . . . . . . . . . . . . . . . . . . . . . . 23

3.3.6 Car Adapter photoLab

6xxx . . . . . . . . . . . . . . 23

4 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

4.1 Switching on or off the photometer . . . . . . . . . . . . . . . . 25

4.2 General operating principles . . . . . . . . . . . . . . . . . . . . . 28

4.2.1 Navigating with function keys and menus . . . . . 28

4.2.2 Display of navigation paths in short form . . . . . 30

4.2.3 Entry of numerals, letters and characters . . . . . 31

4.2.4 Detailed operating example: Changing the

language . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

4.3 Photometer settings and system administration . . . . . . 34

4.3.1 Language . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

4.3.2 Date/Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

4.3.3 Display settings . . . . . . . . . . . . . . . . . . . . . . . . . 36

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Contents photoLab®6100 VIS

4.4 Zero adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

4.5 Measuring in Concentration mode . . . . . . . . . . . . . . . . . 41

4.5.1 Measuring cell tests with barcode . . . . . . . . . . . 41

4.5.2 Measuring reagent tests with AutoSelector . . . . 42

4.5.3 Measuring reagent-free tests and user-defined

methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

4.5.4 Exceeding the upper or lower limits of the

measuring range . . . . . . . . . . . . . . . . . . . . . . . . 46

4.5.5 Selecting a method manually . . . . . . . . . . . . . . . 47

4.5.6 Settings for Concentration mode . . . . . . . . . . . . 48

4.5.7 Measuring diluted samples . . . . . . . . . . . . . . . . 50

4.5.8 Sample blank value . . . . . . . . . . . . . . . . . . . . . . 52

4.5.9 Reagent blank value . . . . . . . . . . . . . . . . . . . . . 54

4.5.10 Automatic Turbidity correction . . . . . . . . . . . . . . 58

4.5.11 Programming / modifying user-defined methods 58

4.5.12 The IQ LabLink procedure . . . . . . . . . . . . . . . . . 68

4.6 Measuring the Absorbance / % Transmission . . . . . . . . 69

4.6.1 General information . . . . . . . . . . . . . . . . . . . . . . 69

4.6.2 Measuring the absorbance or transmission . . . . 69

4.6.3 Measuring against the Reference absorbance . 71

4.7 Multi wavelengths methods . . . . . . . . . . . . . . . . . . . . . . 73

4.7.1 Basic information on Multi wavelengths

measurements . . . . . . . . . . . . . . . . . . . . . . . . . . 73

4.7.2 Programming / modifying Multi wavelengths

methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

4.7.3 Selecting a Multi wavelengths method . . . . . . . 77

4.7.4 Carrying out Multi wavelengths measurements . 78

4.8 Spectrum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

4.8.1 General information . . . . . . . . . . . . . . . . . . . . . . 80

4.8.2 Recording the Spectrum . . . . . . . . . . . . . . . . . . 81

4.8.3 Loading/editing a spectrum . . . . . . . . . . . . . . . . 84

4.8.4 Saving / exporting a spectrum . . . . . . . . . . . . . . 87

4.9 Kinetics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

4.9.1 Creating/editing profiles for kinetic records . . . . 88

4.9.2 Loading a profile for kinetic recording . . . . . . . . 91

4.9.3 Recording the Kinetics . . . . . . . . . . . . . . . . . . . . 92

4.9.4 Saving / exporting a kinetic record . . . . . . . . . . . 95

4.9.5 Loading a kinetic record . . . . . . . . . . . . . . . . . . . 96

4.9.6 Editing a kinetic record . . . . . . . . . . . . . . . . . . . 97

4.10 Timer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

4.10.1 User defined timer . . . . . . . . . . . . . . . . . . . . . . 100

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photoLab®6100 VIS Contents

4.10.2 Analysis timer . . . . . . . . . . . . . . . . . . . . . . . . . 100

4.11 Memory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102

4.11.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102

4.11.2 Instructions on using USB memory devices 104

4.11.3 Measurement datasets . . . . . . . . . . . . . . . . . . 105

4.11.4 Saving measurement datasets manually . . . . 105

4.11.5 Saving measurement datasets automatically . 106

4.11.6 Displaying measurement data memory . . . . . . 107

4.11.7 Filtering measurement datasets . . . . . . . . . . . 109

4.11.8 Inverting filters . . . . . . . . . . . . . . . . . . . . . . . . . 110

4.11.9 Erasing stored measurement datasets . . . . . . 111

4.12 Copying files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112

4.12.1 Copying individual files to a USB memory

device . . . . . . . . . . . . . . . . . . . . . . . . . . 112

4.12.2 Copying all measurement data files to a USB

memory device . . . . . . . . . . . . . . . . . . . . . . . . 114

4.12.3 Copying files to a PC . . . . . . . . . . . . . . . . . . . . 115

4.13 Transmitting data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116

4.13.1 Printer and terminal programs . . . . . . . . . . . . . 116

4.13.2 Settings for data transmission . . . . . . . . . . . . . 116

4.13.3 Printing measurement datasets . . . . . . . . . . . 117

4.13.4 Printing Kinetics records . . . . . . . . . . . . . . . . . 118

4.13.5 Printing spectra . . . . . . . . . . . . . . . . . . . . . . . . 119

4.14 Analytical quality assurance (AQA) . . . . . . . . . . . . . . . 120

4.14.1 General information . . . . . . . . . . . . . . . . . . . . . 120

4.14.2 Photometer monitoring (AQA1) . . . . . . . . . . . . 121

4.14.3 Total system monitoring (AQA2) . . . . . . . . . . . 126

4.14.4 AQA3/MatrixCheck . . . . . . . . . . . . . . . . . . . . . 130

4.15 User management . . . . . . . . . . . . . . . . . . . . . . . . . . . . 136

4.15.1 User levels and user rights . . . . . . . . . . . . . . . 136

4.15.2 Activating or deactivating the User

management function . . . . . . . . . . . . . . . . . . . 137

4.15.3 Creating, changing or deleting a user account 138

4.15.4 Login with active user management . . . . . . . . 141

4.15.5 Changing the password . . . . . . . . . . . . . . . . . 143

4.16 Reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144

4.17 Photometer information ([Info]) . . . . . . . . . . . . . . . . . . 145

4.18 Lamp counter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145

4.19 Software and methods update . . . . . . . . . . . . . . . . . . . 146

4.19.1 Update by means of a USB memory device . . 146

4.19.2 Update by means of a PC . . . . . . . . . . . . . . . . 148

4.19.3 Software update "Chinese character set" . . . . 149

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Contents photoLab®6100 VIS

5 Maintenance and cleaning . . . . . . . . . . . . . . . . . . . . .151

5.1 Replacing the lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151

5.2 Exchanging the buffer batteries . . . . . . . . . . . . . . . . . . 153

5.3 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154

6 What to do if ... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155

6.1 Actions in the case of a broken cell . . . . . . . . . . . . . . . 155

6.2 Error causes and remedies . . . . . . . . . . . . . . . . . . . . . . 156

7 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 159

7.1 Measurement characteristics . . . . . . . . . . . . . . . . . . . . 159

7.2 Measured value documentation and quality assurance 162

7.3 General meter data . . . . . . . . . . . . . . . . . . . . . . . . . . . . 163

8 Accessories and options . . . . . . . . . . . . . . . . . . . . . . .165

8.1 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 165

8.2 Test equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 166

8.3 Optional equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . 166

8.4 Connection cable: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 167

Appendix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 169

A.1 Menus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 169

A.1.1 Measuring . . . . . . . . . . . . . . . . . . . . . . . . . . . . 169

A.1.2 General settings and functions . . . . . . . . . . . . 173

A.2 Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 177

A.3 List of trademarks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 179

3.1 Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 181

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photoLab®6100 VIS Overview

1 Overview

1.1 Overview of the instrument

Front of the

instrument

1Display 2 Keypad 3 Shaft for rectangular cells 4 Turn-up lid 5 Shaft for round cells 6 Cell shaft cover

Fig. 1-1 Front of the instrument with operating elements

Socket field on the

rear panel

7 Connection for power pack 8 RS232 connection 9 USB-A connection 10 USB-B connection

Fig. 1-2 Rear panel with socket field

Note

All connections comply with SELV.

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Overview photoLab®6100 VIS

2 3

1.2 Keypad

Overview

1 Function keys F1 to F4 (function menu-depending) 2 Alphanumeric keypad 3 Keys with dedicated function

Fig. 1-3 Keypad

Key functions The keys on the right side of the keypad have the following functions:

Key Designation Functions

<ON/OFF> – Switches on and off the

photometer.

<HOME> – Switches to the main menu from

any operating situation. Actions that are not completed are canceled.

<PRINT> – Downloads the displayed mea-

sured value to an interface if the Printer symbol is displayed in the status line.

<STORE> – Saves a displayed measured

value or spectrum if the Save symbol is displayed in the status line.

<ZERO·BLANK> – Starts one of the following

measurements, depending on the operating situation:

- Zero adjustment

- Blank value measurement

- Baseline measurement.

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photoLab®6100 VIS Overview

Key Designation Functions

<TIMER> – Opens the menu, Timer.

<ESC> – Cancels the running action.

Entries that have not yet been accepted are discarded.

– Switches to the next higher menu

level.

<START·ENTER> – Starts an action (e.g.

measurement)

– Opens a selected menu

– Confirms a selection or entry

<▲>or

<▼>

– Moves the selection in menus and

lists one position up or down

<W> – Deletes the character left of the

cursor during character entries

– Moves the cursor to the left in a

spectrum or kinetic diagram

(Arrow keys)

<X> – Moves the cursor to the right in a

spectrum or kinetic diagram

Function keys The function keys F1 to F4 have different functions depending on the

operating situation. The current functions are displayed in the function key menu at the bottom edge of the display (see section 4.2.1).

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Overview photoLab®6100 VIS

Concentration

04/16/07 9:52

1.92

mg/l

18: 14752

NH4-N

10 mm

0.05 - 3.00 mg/l

Setup

Method list

Citation form Unit

1.3 Display

Display elements

1 Status line (current state, date and time) 2 Display range for menus and measurement results 3 Function keys menu

Bild 1-4 Display

Symbols in the

status line

Symbol Designation Function

Save The <STORE> key is active.

You can store the displayed data with <STORE> (see section 4.11).

Printer The <PRINT> key is active.

You can output to an interface the displayed data with <PRINT> (see section 4.13).

Progress bar During the warm-up time (15 minutes) a prog-

ress bar appears on the display. The reproducibility of measured values is limited during the warm-up time (see section

4.13).

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photoLab®6100 VIS Safety instructions

2 Safety instructions

This operating manual contains basic instructions that you must follow during the commissioning, operation and maintenance of the photometer. Consequently, all responsible personnel must read this operating manual carefully before working with the meter. Keep this operating manual in the vicinity of the meter.

General safety

instructions

Other labels

Safety instructions in this operating manual are indicated by the warning symbol (triangle) in the left column. The signal word (such as "caution") indicates the danger level:

Warning

indicates instructions that must be followed precisely in order to prevent serious dangers to personnel.

Caution

indicates instructions that must be followed precisely in order to avoid slight injuries to personnel or damage to the instrument or the environment.

Note

indicates notes that draw your attention to special features.

Note

indicates cross-references to other documents.

2.1 Target group and user qualification

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The photometer was developed for use in the laboratory. Carrying out photometric determinations with the aid of test sets frequently requires the handling of hazardous substances.

We assume that the operating personnel know how to handle hazardous substances due to their professional training and experience. The operating personnel must particularly be able to understand and correctly implement the safety labels and safety instructions on the packages and inserts of the test sets.

Safety instructions photoLab®6100 VIS

2.2 Authorized use

The authorized use of the photometer consists exclusively of the carrying out of photometric measurements according to this operating manual. Follow the technical specifications of the cells in chapter 7 T

ECHNICAL DATA. Any other

use is considered to be unauthorized.

2.3 General safety instructions

The photometer is built and inspected according to the relevant guidelines and norms for electronic instruments (see chapter 7 T the factory in a safe and secure technical condition.

Note

Opening the photometer or adjustment and repair work must only be performed by specialist personnel authorized by the manufacturer. Noncompliance invalidates any claim with regard to the warranty.

ECHNICAL DATA). It left

Function and

operational safety

The smooth functioning and operational safety of the photometer can only be guaranteed if the generally applicable safety measures and the specific safety instructions in this operating manual are followed during operation.

The smooth functioning and operational safety of the photometer can only be guaranteed under the environmental conditions that are specified in chapter 7 T

ECHNICAL DATA.

If the photometer was transported from a cold environment to a warm environment, the formation of condensate can lead to the faulty functioning of the meter. In this event, wait until the temperature of the meter reaches room temperature before putting the meter back into operation.

Safe operation If safe operation is no longer possible, the photometer must be taken out of

operation and secured against inadvertent operation.

Safe operation is no longer possible if the photometer:

z has been damaged in transport

z has been stored under adverse conditions for a lengthy period of time

z is visibly damaged

z no longer operates as described in this manual.

If you are in any doubt, contact the supplier of your photometer.

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photoLab®6100 VIS Safety instructions

2.4 Handling of hazardous substances

When developing test sets, WTW carefully sees that the tests can be carried out as safely as possible. Some hazards by dangerous substances, however, cannot always be avoided.

Warning

Improper handling of certain reagents can cause damage to your health. In any case follow the safety labels on the packing and the safety instructions of the package insert. Protective measures specified there have to be followed exactly.

Safety datasheets The safety datasheets of the chemicals comprise all instructions on safe

handling, occurring hazards, preventive actions and actions to take in hazardous situations. Follow these instructions in order to work safely.

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Safety instructions photoLab®6100 VIS

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photoLab®6100 VIS Commissioning

3 Commissioning

3.1 Scope of delivery

z Spectrophotometer photoLab®6100 VIS

z Power pack connection cable

z Buffer batteries 4 x AA alkaline manganese (Mignon)

z Zero cell (16 mm, round)

z Short instructions

z CD-ROM with

– Detailed operating manual

– Analysis instructions

– SpectralTransfer software

– Software update "Chinese character set" (see section 4.19.3)

Packing This photometer is sent out in a protective transport packing.

Caution

Keep the original packing including the inner packing to protect the instrument against hard shocks if it has to be transported. The original packing is also required for the proper return of the instrument if it has to be repaired. Note that damage caused by improper transport voids all warranty claims.

3.2 Initial commissioning

Perform the following activities:

z Insert the buffer batteries (see section 3.2.1)

z Connect the power supply (see section 3.2.2)

z Switch on the photometer (see section 3.2.3)

z Set the language (see section 3.2.4)

z Set the date and time (see section 3.2.5)

z Carry out a zero adjustment (see section 4.4)

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Note

When you set the language, date and time according to the sections and of this operating manual you will quickly become familiar with the simple

operation of the photoLab operation are given in section 4.2 G

6100 VIS. More detailed instructions on

ENERAL OPERATING PRINCIPLES.

Commissioning photoLab®6100 VIS

3.2.1 Inserting the buffer batteries

The buffer batteries supply the integrated clock while the photometer is switched off. Four alkaline manganese batteries (type AA or Mignon) separately included in the scope of delivery are used as the buffer batteries.

Insert the batteries as follows:

1 Turn the photometer upside down

and place it on a soft surface.

2 Open the lid of the battery

compartment (1).

3 Insert the four batteries in the

battery compartment. Make sure that the poles of the batteries are in the correct position. The ± signs on the batteries must correspond to the ± signs in the battery compartment.

4 Close the lid of the battery

compartment.

Battery service life The power consumption of the clock is very low. The lifetime of high quality

batteries is at least 5 years.

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photoLab®6100 VIS Commissioning

3.2.2 Connecting the power supply

The power is supplied with the aid of the enclosed plug-in power pack. The power pack supplies the photometer with low voltage (12 VDC).

Caution

The line voltage of the usage location must fulfill the specifications stated on the power pack (the specifications are also given in chapter 7 T

ECHNICAL DATA). Always use the supplied 12 V original power pack

only.

Connecting the

plug-in power pack

Operation with a

mobile 12 V power

source

1 Connect the miniplug of the power

pack to the socket (1) of the photometer.

2 Connect the power pack to an

easily accessible power socket.

The display illumination switches itself on and then off again.

You can also operate the photoLab

6100 VIS on the move and independent

of the local power supply.

To do so, a 12 V power supply such as a commercial 12 V portable power

source or a 12 V car battery and the Car Adapter photoLab

6xxx available

as an accessory is required (see section 8.1).

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More details on operation are given:

z in section 3.3.6 and

z with the Car Adapter photoLab

6xxx.

Commissioning photoLab®6100 VIS

Language

16.04.07 9:52

Deutsch ✓

English Français Español Italiano Bulgarian/Български Česko Chinese/ 中文 Traditional Chinese/ 繁體中文 Greek/Ελληνικά Indonesian/Indonesia

Language

16.04.07 9:52

Deutsch ✓

English Français Español Italiano Bulgarian/Български Česko Chinese/ 中文 Traditional Chinese/ 繁體中文 Greek/Ελληνικά Indonesian/Indonesia

3.2.3 Switching on the photometer for the first time

During the initial commissioning, the photometer automatically guides you through the setting of the meter language, date and time after switching on (see following sections).

1 Press <ON/OFF>.

The photometer is switched on.

The display switches to the setting of the language (see section

3.2.4).

After the setting of the language the photometer carries out the self-test.

When the initial commissioning is completed, the photometer displays the Home menu each time after it is switched on and after the self-test (see section 4.1).

3.2.4 Setting the language

During the initial commissioning the photometer automatically guides you to the setting of the meter language after switching on.

1 Select a language with <▲><▼>.

2 Confirm the selected language

with <START·ENTER>.

The language has been set. The currently selected language is marked by a tick.

The display switches to the setting of the Date and Time (see section

3.2.5).

After the initial commissioning, you can change the language in the General setup / Language menu at any time (see section 4.2.4).

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photoLab®6100 VIS Commissioning

Date/Time

16.04.07 9:52

Date 16.04.2007

Time 9:52:09

Date/Time

16.04.07 9:52

Date 16.04.2007

Time 9:52:09

Date

23 .10.2006

Date/Time

16.04.07 9:52

Date 16.04.2007

Time 9:52:09

Time

:22 :09

3.2.5 Setting the date and time

During the initial commissioning, the instrument automatically guides you to the setting of the time and date after the setting of the language.

The Date/Time menu is open.

Using <▲><▼>, select a menu item and confirm or open it with

<START·ENTER>

1 Select and confirm Date.

The input field for the current date pops up.

2 Enter the current date with <0...9>

and confirm.

The input field closes. The date is accepted.

3 Select and confirm Time.

The input field for the current time pops up.

4 Enter the current time with <0...9>

and confirm.

The input field closes. The time is accepted.

After the initial commissioning, you can change the date and time in the General setup / Date/Time menu at any time (see section 4.2.4).

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Commissioning photoLab®6100 VIS

USB-A

RS232

USB-B

3.3 Connecting optional accessories

3.3.1 Communication interfaces

Connections

Fig. 3-1 Communication interfaces on the rear panel

You can connect the following accessories to the photometer:

z PC (see section 3.3.2)

z Printer (see section 3.3.2)

z USB storage media (see section 3.3.3)

z USB-PC keyboard (see section 3.3.4)

z Barcode reader (see section 3.3.5)

z Car Adapter photoLab

6xxx (see section 3.3.6)

Note

If you want to connect several USB devices such as a USB-PC keyboard and a USB memory device to the meter, you can increase the number of USB-A sockets by a commercially available USB-2 hub with separate power supply.

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photoLab®6100 VIS Commissioning

3.3.2 PC/printer

PC and printer can be connected to the photometer as follows:

Interface PC Printer Functions

RS232

USB-A

USB-B





 -

The data is sent to the interface with <PRINT>.

z If a printer is connected the data is

printed out.

z If a PC is connected, the data can be

received with a terminal program (see section 4.13).

The data is printed out with <PRINT>.

Enables the direct connection of photometer and PC. With this you can transmit measurement data to the PC (see section 4.12section 4.13) or update the photometer software (see section

4.19.1).

The direct connection with the PC is established with the aid of the "SpectralTransfer" program. The program is provided on the supplied CDROM.

More instructions on how to establish the connection are given in the operating manual of the "SpectralTransfer" program (see CDROM).

ba75847e01 08/2009

Note

Suitable are all printers that can interpret the PCL-3 printer control language.

Commissioning photoLab®6100 VIS

Operation at RS232 Connect the RS232 interface to the devices as follows:

z PC: with a commercially available zero modem cable

z Printer: with a commercially available RS232 printer cable

The cables are available in specialized computer shops.

Set up the following interface data at the PC/printer:

Baud rate Selectable from 1200, 2400, 4800, 9600, 19200

The baud rate must agree with the baud rate set on the PC/printer.

Flow control

none

("handshake")

Parity none

Data bits 8

Stop bits 1

3.3.3 USB memory device

Using a USB memory device (such as a USB memory stick), you can

z Update the meter software and method data (section 4.19)

z Transmit data to the USB memory device (section 4.11)

USB memory devices are connected to the USB-A interface.

Note

Follow the instrucions on the use of USB storage media (see section 4.11.2).

3.3.4 PC keyboard

With the PC keyboard it is possible to enter letters, e.g. to assign names for identification (ID).

In addition, the following keys of the PC keyboard are assigned with the following functions of the photometer:

PC keyboard Photometer

Enter <START·ENTER>

Esc <ESC>

F1 to F4 Function keys <F1> to <F4>

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photoLab®6100 VIS Commissioning

12 V power supply unit (e.g. portable power source or car battery )

photoLab

6100 VIS

Car Adapter photoLab

6xx

The USB-PC keyboard is connected to the USB-A interface.

3.3.5 Barcode reader

The barcode reader enables the simplified entering of alphanumerical character strings and can be used in all operating situations that require the entry of text or numerals. The barcode reader is connected to the USB-A interface.

3.3.6 Car Adapter photoLab

With the Car Adapter photoLab

6xxx

6xxx, you can operate the Pharo 6100 and Pharo 6600 spectrophotometers on the move and independent of the local power supply. To do so, a 12 V power supply such as a commercial 12 V portable power source or a 12 V car battery is required.

Safety instructions For operation with an external battery, follow the safety instructions of the

battery. Make sure the power source is suitable for operation of the spectrophotometer (see technical data of the power source and technical data of the spectrophotometer).

Operating time

with a battery

The maximum operating time depends on various factors:

z Battery (e.g. nominal capacity, condition, age)

z Operating mode of the photometer (e.g. frequency of measurements)

Example:

z Photometer (instrument type)

Operating time with a 12 V / 19 Ah type battery in optimum condition: approx.

6.5 h

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Commissioning photoLab®6100 VIS

Note

The spectrophotometer consumes electricity even while it is in standby mode. We recommend to disconnect the photoLab

6100 VIS if you do not use the

spectrophotometer during battery operation.

Technical data

Car Adapter

photoLab

6xxx

Cable length 2 m

Max. voltage 12 V

Max. current 8 A

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photoLab®6100 VIS Operation

Self test

04/16/07 9:52

Please make sure no cell is inserted and the cover is closed.

Then press <START/ENTER>

Setup Info

04/16/07 9:52

Enter user name

Administrator

Self test

04/16/07 9:52

Please make sure no cell is inserted and the cover is closed.

Then press <START/ENTER>

4 Operation

4.1 Switching on or off the photometer

Switching on

1 Switch the photometer on with

<ON/OFF>.

The display shows

– the Self test dialog (if the user

management is not active).

– the Login dialog

(if the user management is active).

With activated user management:

2 Login

Enter user name and password or register as a guest (see section

4.15.4).

Then the photometer displays the Self test dialog.

Starting the

Self test

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3 Remove all cells and close the cell

shaft cover.

4 Start the self-test with

<START·ENTER>.

The photometer carries out the self-test.

Operation photoLab®6100 VIS

Self test

04/16/07 9:52

Keep cover closed

System test Filter test Lamp test Wavelength calibra tion

Progress bar during warm-up time

Self test During the self-test, all cells must be removed and the cell shaft cover closed.

The self-test includes:

– the test of the memory,

processor, internal interfaces, filter and lamp

– a calibration for each

wavelength

After the self-test is completed, the main menu is displayed.

Note

The result of the self-test can be viewed and printed with the [Info] function key (see section 4.17).

Warm-up time After switching on the photometer requires a warm-up time of 15 minutes.

Reproducibility of measurement data is restricted during the warm-up time. Therefore, do not measure during the warm-up time.

During the warm-up time, a progress bar appears on the display next to the date. The progress bar disappears as soon as the warm-up time is over.

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photoLab®6100 VIS Operation

AutoCheck With the AutoCheck function the photometer checks and calibrates the

optical measuring unit. The AutoCheck is automatically carried out if measurement settings were changed since the last measurement, e.g.:

z if a different wavelength was selected or

z if a different method was selected.

If necessary, the photometer asks you to remove the cell from the cell shaft.

With unchanged measurement settings, the AutoCheck is carried out in the background at regular intervals of 5 minutes. The AutoCheck can only be carried out in the background if the cell shaft is empty. If a cell is in the cell shaft the AutoCheck is carried out only after the cell was removed.

Note

Remove the cell from the cell shaft after every measurement. Thus the photometer can carry out the regular AutoCheck. Cells must be completely removed from the cell shaft. Cells that are removed only half disturb the AutoCheck measurement and, as a consequence, falsify measured values until the next AutoCheck is carried out. Plastic cells that are not recognized by the automatic cell recognition also disturb the AutoCheck.

Note

During a running kinetic measurement the photometer cannot carry out any AutoCheck. That is why in this case a warm-up time of two hours is required. After this time the signal is stable enough so that the measurement accuracy is secured over a longer period of time.

Display illumination The photometer automatically switches off the display illumination if no key

has been pressed for 5 minutes. The illumination is switched on again with the next keystroke. The function of the key becomes active only with the following keystroke.

Switching off To switch the photometer off, keep the <ON/OFF> key depressed until the

photometer is switched off.

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Operation photoLab®6100 VIS

Press twice<>q

Moves the selection down by 2 positions

Current selection in reverse video

Confirm selection with <START ENTER>

Further navigation

andwith <><>pq

Further navigation with function keys (here: F1 and F2)

Press the F1 Funktion key ("Settings")

Opens the "Settings" submenu

Updated function key menu in the multi wavelengths mode

Function key menu

Main menu

4.2 General operating principles

4.2.1 Navigating with function keys and menus

Bild 4-1 Example of navigation with function keys (left) and "classical" menu navigation

(right)

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photoLab®6100 VIS Operation

Use of the function

keys

Navigation with

arrow keys

(<▲><▼>) and

<START·ENTER>

The function keys F1 to F4 are below the display. Their functions change depending on the operating situation and mode. The current functions are displayed in the function key menu at the bottom edge of the display.

Apart from navigation, the function keys are also used for other operations:

z Opening a selection list or input field

z Executing a command (directly or with intermediate query)

z Switch over between two display options,

such as absorbance

Q transmission

These operating elements are used to select an item from a menu or list. The current selection is displayed in reverse video. Pressing of <START·ENTER> confirms the selection.

Apart from navigation, the <START·ENTER> key is also used for other operations:

z Opening a selection list or input field

z Confirming a selection

z Confirming entries of text and numerals

z Executing a command (directly or with intermediate query)

z Activating an item in a selection list (✓ = active)

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Operation photoLab®6100 VIS

<HOME>

[General setup]

– Language

Bold letters and angle brackets indicate a key on the photometer (except function keys).

→ Press the "Home" key.

The main menu is called up.

Square brackets indicate a function key F1 to F4. The text between the brackets corresponds to the assignment according to the function key menu on the bottom edge of the display.

→ Press the function key with the

assignment "Settings"

Text without brackets stands for a menu item indicated on the display (list item).

→ Select the menu item with the

arrow keys <S><T>. The current selection is displayed in reverse video.

→ Then press

<START·ENTER>.

4.2.2 Display of navigation paths in short form

In this operating manual, the introductory navigation steps leading to individual menus or dialogs are clearly shown in a gray box. The box indicates a section of the menu tree.

Starting point of the description is always the main menu, which can be reached with the <HOME> key from any operating situation. From there navigation takes place downward.

Operating example:

Navigation to the

setting menu for the

The following example shows the elements of the menu tree with the relevant operating steps:

language

Further navigation options:

z The <ESC> key moves you one level up in the menu tree.

z The <HOME> key directly calls up the main menu.

Note

If you are "lost" in a menu, press <HOME> and restart navigating from the main menu.

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photoLab®6100 VIS Operation

Note

The complete menu tree is given in the appendix of this operating manual.

4.2.3 Entry of numerals, letters and characters

Numerals, letters, punctuation marks and special characters are entered with the alphanumeric keypad of the meter or using an external keyboard. Entries are required in operating situations such as the following:

z Entering the date and time

z Entering an ID e.g. when storing measurement data

z Selecting a method with the [Search] function

z Programming user-defined methods

z Entering user name and password

z Administrating users

Character set The following characters are available:

z Numerals 0 ... 9

z Letters A ... Z and a ... z

z Punctuation marks. -

z Special characters ° / + ² ³ # %

Operating principle Entering characters is always possible if there is an input field on the display.

The numerals and characters (expect for the small letters) assigned to the keys of the alphanumeric keypad are printed on the keys. Example: With the <7/PQRS> key you can enter the following characters: 7, P, Q, R, S, p, q, r, s.

Select the required character by pressing the key several times (similar to a mobile phone). When pressing a key that is assigned to several characters once, the respective numeral appears first. To enter a numeral, one keypressing is always sufficient.

When pressing the key for the first time a line pops up that displays all characters possible with this key. The currently selected character is highlighted.

A character is taken over in the input field if

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z the character is highlighted for more than one second,

z the character is confirmed with <START·ENTER>,

z another alphanumeric key is pressed.

Operation photoLab®6100 VIS

Enter ID

8 T U V t u v

Enter ID

T U V t u v

Enter ID

Tes t_

Note

During mere number entries (such as entering a wavelength), the keys of the alphanumeric keypad are assigned to the respective numeral only. Each keypressing directly enters the numeral (like a pocket calculator).

Special characters Special characters are entered with the <1/*> key.

Operating example:

Entering the ID

Correcting incorrect

entries

The Enter ID input field appears if you press the <STORE> key while the storing symbol is visible. In the following example a measurement dataset with the ID "Test" is stored.

1 Press <8/TUV> several times until

"T" appears in the input line.

Below the input field, a selection line pops up with all characters that are available for this key, e.g. 8 T U V t u v. The currently selected character is highlighted.

After approx. one second the character is taken over and the selection line closed.

2 Complete the ID with <A...9> and

confirm.

Using <W>, erase all characters until you have reached the incorrect digit and repeat the entry from there.

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photoLab®6100 VIS Operation

General setup

04/16/07 9:52

Language

Date/Time Display settings User managementg Measured value memory Software/methods up date Reset Data transfer/Printer Save all data on USB memory

Language

16.04.07 9:52

Deutsch ✓

English Français Español Italiano Bulgarian/Български Česko Chinese/ 中文 Traditional Chinese/ 繁體中文 Greek/Ελληνικά Indonesian/Indonesia

Deutsch ✓

4.2.4 Detailed operating example: Changing the language

1 Call up the main menu with the

<HOME> key.

2 Open the General setup menu

with the F1 function key [Setup].

3 Using <S><T>

, select the

Language menu item and open with <START·ENTER>.

The Language menu shows a list with the available languages. The currently active language is marked by a tick.

4 Select the required language from

the list with <S><T> and confirm with <START·ENTER>.

The selected language is taken over immediately. The photometer moves up one menu level.

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Operation photoLab®6100 VIS

4.3 Photometer settings and system administration

The general photometer settings are done in the <HOME> -> General setup menu. The general photometer settings comprise:

z Language (see section 4.3.2)

z Date/time (see section 4.3.2 and section 4.2.4)

z Display characteristics (see section 4.3.3)

z User management (see section 4.15)

z Administration of the measurement data memory (see section 4.11)

z Software and method update (see section 4.19)

z Reset of the settings to default values (see section 4.16)

z Settings for data transmission (see section 4.13.2)

4.3.1 Language

The complete list of the available instrument languages is given in the Sprache/LanguageLanguage menu of the photometer and in chapter 7 T

NICAL DATA.

ECH-

Note

You can select the "Simplified Chinese/ 中文 " or "Traditional Chinese/ 繁體中文 " language only if the Chinese character set has been previously installed

(see section 4.19.3).

Note

How to set the language is described in detail in the operating example in section 4.2.4.

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photoLab®6100 VIS Operation

<HOME>

[General setup]

– Date/Time

Date/Time

04/16/07 9:52

Date 16.04.2007

Time 9:52:09

Date

23 .10.2006

Date/Time

04/16/07 9:52

Date 16.04.2007

Time 9:52:09

Time

:22 :09

4.3.2 Date/Time

The date format is set automatically with the language setting. According to the locally usual version, the date format is displayed in the order, Day.Month.Year (DD.MM.YY) or Month/Day/Year (MM/DD/YY or MM.DD.YY).

The Date/Time menu is open.

1 Select and confirm Date.

The input field for the current date pops up.

2 Enter the current date with <0...9>

and confirm.

The input field closes. The date is accepted.

3 Select and confirm Time.

The input field for the current time pops up.

4 Enter the current time with <0...9>

and confirm.

The input field closes. The time is accepted.

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Operation photoLab®6100 VIS

<HOME>

[General setup]

– Display settings

Display settings

04/16/07 9:52

Contrast 50 %

4.3.3 Display settings

Here you can adjust the display contrast to the lighting conditions.

1 Select and confirm Contrast.

A slide control for the display contrast appears.

2 Using <W><X>, set the display

contrast and confirm.

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photoLab®6100 VIS Operation

4.4 Zero adjustment

A valid zero adjustment is required for the calculation of measured values in the modes, Concentration, Absorbance / % Transmission, Multi wavelengths and Kinetics. With a zero adjustment, the absorbance of a cell filled with distilled water ("zero cell") is measured and stored.

Zero adjustment for

absorbance

measurements

Factory zero

adjustment for

concentration

measurements

Notes on zero

adjustment

The zero adjustment has to be carried out separately for each cell type and each used wavelength. If necessary, the photometer reminds you that a zero adjustment is due.

For all measurements with WTW test sets (Concentration mode), a factory zero adjustment is available in the delivery condition. We recommend replacing it with a zero adjustment of your own.

Note

The cells must be absolutely clean and free of scratches. Always use a cell of the same type for zero adjustment and measurement of the sample.

Zero adjustment with round cells:

z Only use clean, scratch-free round cells with distilled water. The minimum

filling level is 20 mm. A ready zero cell is included in the scope of delivery of the photometer and PhotoCheck (see chapter 8 A

OPTIONS).

CCESSORIES AND

z A ready zero cell can, in principle, be used any number of times. We

recommend, however, to regularly check the zero cell for visible contamination and scratches and refill or exchange it if necessary (at least every 24 months).

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Zero adjustment with rectangular cells:

z For rectangular cells, the zero adjustment must be carried out with the

same cell type (manufacturer and glass type [e.g. optical glass, quartz glass]) that is used for measurement. This is important because cells of different manufacturers have different absorption behavior. When changing the cell type repeat the zero adjustment with the new type.

z Prior to zero adjustment, clean the rectangular cell and fill it with distilled

water. The minimum filling level is 20 mm.

z Rectangular cells always have to be inserted in the cell shaft with the

same orientation for measurement and zero adjustment (e.g. cell printing on the left side ).

Operation photoLab®6100 VIS

Concentration

04/16/07 9:52

3: A6/25

NH4-N

16 mm

0.20 - 8.00 mg/l

Setup

Method list

Citation form Unit

Adjust

Blank value

Zero adj ust men t

Zero adju stm ent

04/16/07 9:52

Please insert zero cell (distilled water) or press <Start/Enter>

Zero cell (H O dist.)

inner turn-up lid

Note

Ordering information is given in chapter 8 A cells listed in the chapter 8 A

CCESSORIES AND OPTIONS are especially adapted

CCESSORIES AND OPTIONS. The

to the WTW test set program. General requirements of the cells are given in chapter 7 T

ECHNICAL DATA. Note that the spectral transparency of the cell

must be suitable for the intended application (example, quartz cell for UV range).

Carrying out a zero

adjustment

The zero adjustment takes place similarly in the Concentration, Absorbance / % Transmission, Multi wavelengths and Kinetics modes.

1 In the respective mode, press the

<ZERO·BLANK> key.

2 In Concentration mode only:

Select and confirm Zero adjustment.

The zero adjustment window pops up.

3 Close the inner turn-up lid.

4 Depending on the cell type, insert

the zero cell as follows:

Round cell:

Insert the round cell in the round cell shaft so it touches the bottom.

If the inner turn-up lid is opened too wide, a message prompts you to close the inner turn-up lid.

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photoLab®6100 VIS Operation

Zero cell (H O dist.)

Zero adju stm ent

04/16/07 9:52

Zero adjustment successful

10 mm

Rectangular cell:

Open the inner turn-up lid.

Insert the rectangular cell vertically so it touches the bottom and left edge of the cell shaft. The opaque sides of the rectangular cell must point to the front and back.

The photometer has an external light recognition. If there is too much external light, a message prompts you to close the cell shaft cover.

The photometer automatically starts the zero adjustment and subsequently stores the value.

5 After a successful zero adjustment

switch to measurement with [OK].

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Operation photoLab®6100 VIS

Validity of the zero

adjustment

The data of the zero adjustment is stored in the photometer separately for each cell type. As long as the data is valid, it is automatically used again after a temporary change to a different cell type. The validity depends on the respective mode:

Mode Validity of the zero adjustment

Concentration (permanently

z Till the next zero adjustment

programmed methods)

Absorbance / % Transmission z Till the next zero adjustment with the

same wavelength *

Concentration (user-defined methods) and

z Till the next zero adjustment for the same

method *

Multi wavelengths

Kinetics z Till another kinetic profile is loaded

z Till the Kinetics mode is exited or the

photometer is switched off

* After the wavelength or method respectively was temporarily exited the photometer

displays that a zero adjustment is available and the time it was carried out. You can then decide whether to use this zero adjustment or carry out a new zero adjustment.

When to repeat the

zero adjustment?

We recommend to repeat the zero adjustment in the following cases:

z If the photometer was subject to mechanical stress such as strong shock

or transport

z If the ambient temperature changed by more than 5 °C since the last zero

adjustment

z After the lamp was replaced

z At least once per week

z If a new cell type (different manufacturer, different glass type is used)

z Basically each time you want to measure with the highest possible

accuracy.

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photoLab®6100 VIS Operation

<HOME>

Concentration

04/16/07 9:52

Please select method for measuring or insert a barcoded cell

or insert AutoSelector.

Setup

Method list La st method New Method

Line mark

Barcode

Concentration

04/16/07 9:52

mg/l

18: C3/25

COD

16 mm

10 - 150 mg/l

Setup

Method list

Citation form Unit

4.5 Measuring in Concentration mode

4.5.1 Measuring cell tests with barcode

Inserting a cell with barcode starts a measurement.

1 Open the cell shaft cover.

2 Close the inner turn-up lid.

If the inner turn-up lid is opened too wide, a message prompts you to close the inner turn-up lid.

3 Insert the barcoded round cell in

the round cell shaft so it touches the bottom. When doing so, align the line mark with the notch at the front of the round cell shaft.

The photometer selects the method based on the bar code and automatically starts measurement.

4 Further options:

– Select a different citation form

with [Citation form], (e.g. NH

<–> NH4-N).

– Select a different measuring

unit with [Unit], (e.g. mg/l <–> mmol/l).

– Make further settings such as

dilution or blank value measurements with [Setup] (see section 4.5.6).

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Display if the value is not within the measuring range (see section 4.5.4).

Operation photoLab®6100 VIS

<HOME>

Concentration

04/16/07 9:52

Please select method for measuring or insert a barcoded cell or insert AutoSelector.

Setup

Method list La st method New Method

Concentration

04/16/07 9:52

To start measurement, insert cell or press <Start/Enter>

38: 14761

10 mm

0.05 - 5.00 mg/l

Setup

Method list

Citation form Unit

Line mark

Barcode

inner turn-up lid

4.5.2 Measuring reagent tests with AutoSelector

The method is selected by inserting the AutoSelector.

The photometer is ready to measure.

1 Open the cell shaft cover.

2 Insert the AutoSelector in the

round cell shaft so it touches the bottom. When doing so, align the line mark with the notch at the front of the round cell shaft.

– The photometer selects the

correct method with the aid of the barcode.

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photoLab®6100 VIS Operation

Concentration

04/16/07 9:52

1.92

mg/l

18: 14752

NH4-N

10 mm

0.05 - 3.00 mg/l

Setup

Method list

Citation form Unit

3 Open the inner turn-up lid.

4 Insert the rectangular cell

vertically so it touches the bottom and left edge of the cell shaft. The opaque sides of the rectangular cell must point to the front and back.

The correct measuring range is automatically selected when the rectangular cell (1, 2, 5 cm) is inserted.

The photometer has an external light recognition. If there is too much external light, a message prompts you to close the cell shaft cover.

The photometer starts measuring automatically.

5 Further options:

– Select a different citation form

with [Citation form], (e.g. NH

<–> NH4-N).

– Select a different measuring

unit with [Unit], (e.g. mg/l <–> mmol/l).

– Make further settings such as

dilution or blank value measurements with [Setup] (see section 4.5.6).

Display if the measured value is not within the measuring range (see section

4.5.4).

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Operation photoLab®6100 VIS

<HOME>

Concentration

04/16/07 9:52

Please select method for measuring or insert a barcoded cell

or insert AutoSelector.

Setup

Method list La st method New Method

Concentration

04/16/07 9:52

To start measurement, insert cell or press <Start/Enter>

3: A6/25

NH4-N

16 mm

0.20 - 8.00 mg/l

Setup

Method list

Citation form Unit

inner turn-up lid

4.5.3 Measuring reagent-free tests and user-defined methods

User-defined methods and reagent-free methods normally do not have a barcode and therefore, no automatic method recognition. In such a case, select the method manually:

1 Select the method manually

(see section 4.5.5).

The photometer is ready to measure.

2 Depending on the type, insert the

cell as follows:

Round cell:

Close the inner turn-up lid.

Insert the round cell in the round cell shaft so it touches the bottom.

If the turn-up lid is opened too wide, a message prompts you to close the inner turn-up lid.

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photoLab®6100 VIS Operation

Concentration

04/16/07 9:52

0.629

mg/l

1001: Nitrite

NO2-N

10 mm

0.050 - 1.000 mg/l

Setup

Method list

Citation form Unit

Rectangular cell:

Open the inner turn-up lid.

Insert the rectangular cell vertically so it touches the bottom and left edge of the cell shaft. The opaque sides of the rectangular cell must point to the front and back.

The photometer has an external light recognition. If there is too much external light, a message prompts you to close the cell shaft cover.

3 Further options:

– Select a different citation form

with [Citation form], (e.g. NH

<–> NH4-N).

– Select a different measuring

unit with [Unit], (e.g. mg/l <–> mmol/l).

– Make further settings such as

dilution or blank value measurements with [Setup] (see section 4.5.6).

Display if the measured value is not within the measuring range (see section

4.5.4).

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Operation photoLab®6100 VIS

4.5.4 Exceeding the upper or lower limits of the measuring range

Measured value display if the measured value is outside the measuring range:

Range Display Example:

MR: 10 - 150 mg/l

LL < MV < UL Measured value

1 UL < MV < UL + 10% Upper limit of measuring

range exceeded by up to 10% and measured value

LL - 50% < MV < UL Lower limit of measuring

range undercut by up to 50% and measured value

2MV > UL + 10% Upper limit of measuring

range exceeded by more than 10%

MV < LL - 50% Lower limit of measuring

range undercut by more than 50%

3 Invalid measured value

Lines

e.g. MV < 0

MR = Measuring range

UL = Upper limit value of the measuring range

LL = Lower limit value of the measuring range

MV = Measured value

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photoLab®6100 VIS Operation

<HOME>

Concentration

– [Last method]

<HOME>

Concentration

– [Method list]

Select method (all)

04/16/07 9:52

Search:

1 C3/25 COD 10 - 150 mg/l

2 C4/25 COD 25 - 1500 mg/l 3A6/25 NH4-N 0.20 - 8.00 mg/l 4N2/25 NO3-N 0.5 - 25.0 mg/l 5N5/25 NO2-N 0.010 - 0.700 mg/l 6P6/25 PO4-P 0.05 - 5.00 mg/l 7P7/25 PO4-P 0.5 - 25.0 mg/l 160 09717 Pb 0.10 - 5.00 mg/l 67 14834 Cd 0.025 - 1.000 mg/l 39 14552 Cr 0.05 - 2.00 mg/l

▼

Last used

4.5.5 Selecting a method manually

Selecting the

method last used

Selecting a method

from the Method list

The method last used is immediately selected.

The list of methods is displayed. The methods are ordered according to the method number. The arrows



or  on the right edge indicate that the list comprises more methods further up or down.

The method last selected is highlighted.

Narrowing down the

method list

Select the method:

1 Select the required method with

<▲><▼>. The active selection is

displayed in reverse video.

2 Accept the selection with

<START·ENTER>.

You can narrow down the method list and thus make the search easier:

z Using [Last used], you can restrict the method list to the ten methods last

used.

z With the search function you can search certain character strings in the

list. The search takes place as a full-text search of the entire list contents. Thus you can search for a method number or certain citation form.

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Operation photoLab®6100 VIS

Select method (last use d)

04/16/07 9:52

14 14540 COD 10 - 150 mg/l

23 14541 COD 25 - 1500 mg/l

All methods

CO_

<HOME>

Concentration

Select a method

– [Setup]

Concentration

04/16/07 9:52

Dilution  Sample blank value User-defined blank value Turbidity correction

Display absorbance



AQA Edit method New method Measurement data memory

Search function

Search for a character string:

Enter the character string to be searched for in the search window with <A...9>.

The list appearing below shows all hits containing the character string. The hit list is updated with each character that is entered.

Note

Note the case sensitivity when searching. It is not required or possible to enter inferior characters. When searching for chemical formulas, inferior characters are treated as normal characters. Example: The search for "NH4" shows all hits that contain "NH4" as well as "NH

4.5.6 Settings for Concentration mode

Prior to measuring, check the settings for the selected method.

The menu shows an overview of all settings.

Active settings are marked by a tick.

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photoLab®6100 VIS Operation

Overview of the

settings

Menu item Explanation

Dilution Here you can set the dilution prior to measuring if

you want to use a diluted sample.

In the measured value display, the dilution is indicated in the form [1 + x] (parts sample + parts distilled water).

For more detailed information on dilution, see section 4.5.7.

Sample blank value Here you can measure while taking a sample blank

value into account.

In the measured value display, measurements with sample blank value are marked by [SB] (Sample blank).

For more detailed information on sample blank value, see section 4.5.8.

User-defined blank value

If available, a user-defined reagent blank value is used.

In the measured value display, measurements with a user-defined reagent blank value are marked by [BV/Lot number].

For more detailed information on reagent blank value, see section 4.5.9.

Turbidity correction Activates/deactivates the automatic turbidity

correction.

In the measured value display, measurements with automatic turbidity correction are marked by [TURB].

For more detailed information on the automatic turbidity correction, see section 4.5.10.

Display absorbance Activates/deactivates the display of the

absorbance value in addition to the main measured value.

AQA Here you can view and change the AQA settings

without discarding the current measurement.

Edit method Here you can edit user-defined methods.

New method Here you can create user-defined methods.

Measurement data memory

Here you can view the measurement data memory.

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Operation photoLab®6100 VIS

<HOME>

Concentration

04/16/07 9:52

Please select method for measuring or insert a barcoded cell

or insert AutoSelector.

Setup

Method list La st method New Method

Concentration

04/16/07 9:52

To start measurement, insert cell or press <Start/Enter>

3: A6/25

NH4-N

16 mm

0.20 - 8.00 mg/l

Setup

Method list

Citation form Unit

4.5.7 Measuring diluted samples

If the concentration of a sample exceeds the measuring range of a method, you can specifically dilute the sample so that the concentration of the diluted sample is in the measuring range of the method. Thus a valid measurement is possible.

After entering the factor for the dilution the meter converts the concentration to that of the undiluted sample.

Note

Optimum measurement results are achieved if the concentration of the diluted sample is in the middle of the measuring range of the method after diluting.

Setting the dilution

Inserting a cell with barcode starts a measurement.

If a cell without barcode is used: Select the method manually (see section 4.5.5).

The photometer is ready to measure.

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photoLab®6100 VIS Operation

Concentration

04/16/07 9:52

3: A6/25

NH4-N

16 mm

0.20 - 8.00 mg/l

Setup

Method list

Citation form Unit

Sample + distil led water

1 + _

1 Open the setting menu with

[Setup].

2 Select and confirm Dilution.

The input field for the dilution pops up.

3 Enter and confirm the dilution

(<0...9>).

The entered dilution is taken into account with the next measurement.

The entered value for the dilution factor is valid for the selected method only. The dilution factor is erased if

z the photometer is switched off

z a different method is selected

z the factor 0 is entered in the Dilution menu.

If a dilution factor is active, it is indicated on the display during measurement in the form [1 + x].

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Operation photoLab®6100 VIS

<HOME>

Concentration

04/16/07 9:52

Please select method for measuring or insert a barcoded cell

or insert AutoSelector.

Setup

Method list La st method New Method

4.5.8 Sample blank value

By measuring and using a sample blank value, measurement errors due to coloring and turbidity of the sample matrix can be eliminated to a large extent.

The sample blank value is a characteristic of the sample (coloration) to be currently determined. It is determined by measuring the blank sample.

The blank sample is prepared from the sample by adding reagents to it and is especially adapted to measurement with the test to be measured. No chromophoric reagents, however, are added for the preparation of the blank sample.

Note

Due to the addition of reagents the sample is diluted. This can also change the pH value of the sample. For this reason the blank sample also has to be diluted and the pH value adjusted accordingly.

Validity The sample blank value applies to the next measurement only.

Single and multiple

determination

Measuring the

sample blank value

The sample blank value can be determined by single or multiple determination. With multiple determination, the sample blank value is calculated as the median from the individual measured values.

Inserting a cell with barcode starts a measurement.

If a cell without barcode is used: Select the method manually (see section 4.5.5).

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photoLab®6100 VIS Operation

Concentration

04/16/07 9:52

To start measurement, insert cell or press <Start/Enter>

3: A6/25

NH4-N

16 mm

0.20 - 8.00 mg/l

Setup

Method list

Citation form Unit

Sample blank value

04/16/07 9:52

To start measurement, insert cell or press <Start/Enter>

3: A6/25

NH4-N

16 mm

0.20 - 8.00 mg/l

Sample blank value

04/16/07 9:52

Last measured absorbance

0.115

Median

0.115 (1 Measurement(s))

3: A6/25

NH4-N

16 mm

0.20 - 8.00 mg/l

Next meas.

Discard Apply

Concentration

04/16/07 9:52

[SB]

To start measurement, insert cell or press <Start/Enter>

3: A6/25

NH4-N

16 mm

0.20 - 8.00 mg/l

Setup

Method list

Citation form Unit

The photometer is ready to measure.

1 Open the setting menu with

[Setup].

2 Select and confirm Sample blank

value.

3 Insert the cell with a suitable blank

sample.

The first single measurement for the sample blank value takes place.

The following data is displayed as the result:

– The measured absorbance

from the (last) single measurement.

– The median from all single

measurements carried out up to now.

4 If necessary, carry out further

single measurements for the formation of the median with [Next

meas.]

or discard the last single measurement with [Discard].

5 To accept the median value, press

[Apply].

The photometer is ready to measure.

ba75847e01 08/2009

The use of the sample blank value is indicated by [SB] in the top right corner of the display.

Operation photoLab®6100 VIS

4.5.9 Reagent blank value

The evaluation of the photometric measurement always refers to the comparison value of a test sample without the substance to be determined (reagent blank value). Thus the influence of the basic absorbance of the reagents on photometric measurement is compensated for.

In practice, the reagent blank value is measured with the same amount of deionized water instead of sample.

Factory and user-

defined reagent

blank values

Validity The factory blank values always remain stored in the meter and can be

Single and multiple

determination

With photometric concentration determination, the reagent blank value is a constant. The method data for all measurements with WTW test sets (Concentration mode) include an exactly determined reagent blank value. This value is overwritten if you measure the reagent blank value yourself (setting, User-defined blank value, see section 4.5.6).

Note

You can increase accuracy if you determine the reagent blank value with a test of a new lot and use the reagent blank value for all further measurements with this lot. This is especially recommended for measurements in the vicinity of the lower limit of the measuring range. To be able to attribute the reagent blank value in the measured value documentation later, you can enter the lot number of the reagent package (Lot number) during the blank value determination.

activated at any time. The reagent blank values you measured yourself also remain stored in the meter until they are overwritten by a new blank value measurement.

The reagent blank value can be determined with single or multiple determination. With multiple determination, the reagent blank value is calculated as the median from the individual measured values.

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photoLab®6100 VIS Operation

User-defined

methods

For user-defined methods, you can activate the reagent blank value function as follows only:

Entry type Function type Reagent

blank value possible?

Entry of a function (with and without entering the

Linear Yes

Nonlinear No

ordinate intercept)

Entry of value pairs or measurement and storage of standard solutions

Linear Yes

Parabola

Yes

(second-order function)

(with entering or measuring and storing E0)

Entry of value pairs or measurement and storage of standard solutions (without entering or measuring and storing E0)

Polygon line No

Linear Yes

Parabola

No (second-order function) Polygon line Polygon line through zero

Note

If no value for E0 is stored during the entry of value pairs or the measurement and storing of standard solutions for a nonlinear function (parabola or polygon line), the message, No blank value correction is intended for this method. appears when the User-defined blank value function is activated. The blank value (E0) can be entered later by editing the method.

ba75847e01 08/2009

Operation photoLab®6100 VIS

<HOME>

Concentration

04/16/07 9:52

Please select method for measuring or insert a barcoded cell

or insert AutoSelector.

Setup

Method list La st method New Method

Concentration

04/16/07 9:52

To start measurement, insert cell or press <Start/Enter>

3: A6/25

NH4-N

16 mm

0.20 - 8.00 mg/l

Setup

Method list

Citation form Unit

Concentration

04/16/07 9:52

3: A6/25

NH4-N

16 mm

0.20 - 8.00 mg/l

Setup

Method list

Citation form Unit

Adjust

Zero a djustment

Blank value

Measuring the

reagent blank value

Inserting a cell with barcode starts a measurement.

If a cell without barcode is used: Select the method manually (see section 4.5.5).

The photometer is ready to measure.

1 Using <ZERO·BLANK>, open the

Adjust selection list.

2 Select and confirm Blank value.

The window for the measurement of the reagent blank value pops up. The data of the last measurement appears in the measured value display.

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photoLab®6100 VIS Operation

Blank value

04/16/07 9:52

To start measurement, insert cell or press <Start/Enter>

3: A6/25

NH4-N

16 mm

0.20 - 8.00 mg/l

Blank value

04/16/07 9:52

Last measured absorbance

0.600

Median

0.600 (1 Measurement(s))

3: A6/25

NH4-N

16 mm

0.20 - 8.00 mg/l

Next meas.

Discard Apply

Blank value

04/16/07 9:52

[BV/Lot number]

To start measurement, insert cell or press <Start/Enter>

3: A6/25

NH4-N

16 mm

0.20 - 8.00 mg/l

Setup

Method list

Citation form Unit

3 Insert the cell with the blank

sample.

The first single measurement for the reagent blank value takes place.

The following data is displayed as the result:

– The measured absorbance

from the (last) single measurement.

– The median from all single

measurements carried out up to now.

4 If necessary, carry out further

single measurements for the formation of the median with [Next

meas.]

or discard the last single measurement with [Discard].

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5 To accept the median value, press

[Apply].

The Lot number entry field pops up.

6 Enter and confirm the Lot number

(<A...9>). The blank value measurement is completed.

The photometer is ready to measure.

The use of the reagent blank value is indicated by [BV/Lot number] in the top right corner of the display.

Operation photoLab®6100 VIS

4.5.10 Automatic Turbidity correction

The Turbidity correction function activates the automatic recognition and compensation of the light absorption caused by turbid substances.

After activating the function remains permanently switched on. Measured values that were measured with Turbidity correction are labeled with [TURB] (turbidity correction) on the display and in the documentation (printout and memory).

The Turbidity correction function is not active in the delivery condition.

Note

The setting for automatic turbidity correction is used with all methods where the automatic turbidity correction makes sense. The photometer automatically decides whether or not to use the function.

Switching on the

turbidity correction

Overview For Concentration mode, you can develop and store yourself user-defined

Calibration data and

calibration function

The automatic turbidity correction is activated and deactivated in the setting menu of the concentration measurement (see section 4.5.6 S C

ONCENTRATION MODE).

ETTINGS FOR

4.5.11 Programming / modifying user-defined methods

methods under the method numbers 1001 to 1100. The photometer software supports you when creating the methods.

In photometry, the calibration function describes the dependency between the measured parameter (e.g. concentration) and the photometric measurement result (e.g. absorbance) of a sample. The knowledge of this dependency is a prerequisite for the development of a photometric method. The calibration function is usually determined by means of a series of measurements with standard solutions of known concentrations (nominal value), e.g. a 10-point calibration.

Note

In measuring operation, the reverse calibration function is used to output the measured absorbance as a concentration value.

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photoLab®6100 VIS Operation

Nominal value (e. g. concentration)

Absorbance

Nominal value (e. g. concentration)

Absorbance

Nominal value (e. g. concentration)

Absorbance

Line types The dependency between the nominal value and absorbance is often linear

in a wide range as shown in the following example:

Bild 4-2 Example of a linear calibration function after a 10-point calibration

In the case of a linear dependency, the calibration function is determined by means of linear regression. The slope and axis intercept (E0) are the characteristics of the calibration line.

In the case of a nonlinear dependency, the points of the measuring ranges can be connected to each other as a polygon line or approximated as a parabola:

Bild 4-3 Example of a polygon line calibration function after a 10-point calibration

ba75847e01 08/2009

Bild 4-4 Example of a parabola calibration function after a 10-point calibration

Operation photoLab®6100 VIS

Determining the

calibration function

You have the following options to create a method:

z Measure and store:

Carry out a series of measurements with the following sample solutions while at the same the photometer takes over the values:

– Blank sample to determine the reagent blank value

(with deionized water instead of sample, see section 4.5.9)

– at least one, up to ten standard solutions in different concentrations.

The photometer stores the nominal value/absorbance value pairs of the individual measurements and determines the resultant characteristics of the calibration. When doing so, you can select the following line types: Polygon line, Straight line or Parabola.

z Enter as value pairs:

Entry of the value pairs, Nominal value (concentration) / Measured absorbance of an already available

test series with the following sample

solutions:

– Blank sample to determine the reagent blank value

(with deionized water instead of sample, see section 4.5.9)

– at least one, up to ten standard solutions in different concentrations.

Based on the entered value pairs, the photometer determines the characteristics for the calibration. When doing so, you can select the following line types: Polygon line, Straight line or Parabola.

z Enter a function:

Entry of a function to calculate the concentration from the absorbance (reverse calibration function). You can enter on the photometer the coefficients of a polynomial equation of the following type:

c = a0 + a1·A + a2·A

+ a3·A3 + a4·A4 + a5·A

with:

c Measurement result, e.g. concentration

a0 to a5 Coefficients (input range 0.000 to 1000,000)

A Absorbance

Note

Entering the formula is especially simple if you measure with a commercial test set for which the manufacturer has given the value for the coefficient a1. It is often called the "Factor" and corresponds to the reciprocal value of the slope of the straight line of the calibration function.

If a linear

function (straight line) should be entered, it is necessary to enter

the coefficients a0 and a1 to receive correct measured values.

ba75847e01 08/2009

photoLab®6100 VIS Operation

Linear function

If the value for a1 (slope of the reverse calibration function) is unknown, you can very simply program the method in the photometer by measuring/storing or entering the value pairs (see above).

For entry as a formula, you can determine the coefficients of the reverse calibration function by linear regression. When doing so, the concentration has to be on the Y axis and the absorbance on the X axis.

In the case of a linear function, the coefficients of the reverse calibration function can also be determined from the determined reagent blank value and the slope (m) of the calibration function (Y axis = absorbance, X axis = concentration). Proceed as described below.

Explanation of the coefficients of the formula:

z a0 = - E0*a1

[E0 = reagent blank value (absorbance at concentration 0)]

z a1 = 1/m

Reverse value of the slope of the calibration

function

(often referred to as "Factor") m = slope of the calibration function

z a2, a3, a4, a5 = further coefficients

(when entering a linear function: zero)

Nonlinear function

The coefficients of the reverse calibration function are determined by multiple regression. When doing so, the concentration has to be on the Y axis and the absorbance on the X axis.

If the exact value for a0 is not known at the time the formula is entered, it is sufficient to enter the coefficient a1. In this case, the User-defined blank value function (in the Concentration / Setup menu) has to be activated to measure with this method. Prior to measuring with this method, a blank value measurement has to be carried out in this case. This procedure determines the value for a0, which then replaces the value from the programming of the method. If the User-defined blank value function is not activated, the photometer uses the value zero for the coefficient a0.

More information on

the entry of the

formula

(determination of

coefficients)

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Operation photoLab®6100 VIS

Further method data

Input field Possible entries

Number* 1001 ... 1100

Designation Any name (max. 18 characters)

Version Any version designation (max. 18 characters)

Wavelength* Freely selectable (in nm)

Cell* 16 (round), 10, 20 or 50 mm

Citation form e.g. PO4-P (max. 18 characters)

Unit** e.g. mg/l (max. 18 characters)

Resolution* 0.001, 0.01, 0.1 or 1

Lower and upper limit of the measuring range *

Any value between zero and the highest concentration of the used standard solutions

Timer 0 to 3 Up to four analysis timers freely adjustable

AQA2 target value Any value within the measuring range

AQA2 tolerance Any

* necessary inputs ** default: mg/l

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photoLab®6100 VIS Operation

<HOME>

Concentration

– [Setup]

– New method

Edit method

04/16/07 9:52

Number 1001 Designation Nitrite Ver sion 01 Wavelength 525 Cell 10 mm Citation form NO2-N Unit mg/l Resolut ion 0.001 Calibration curve Measure standard solutions

Method list Delete Ne xt

How to program

user-defined

methods

1 Enter the general method data

here. The next available method number is already entered as the number.

You have the following options when filling out the input fields:

- Fill out all empty input fields one after the other

- Using [Method list], select an already existing method as a model, give it a new method number and adjust the entries

- Using [Method list], select an existing method in order to change it (without changing the number).

- You can delete the method completely with [Delete].

2 Select the menu item, Calibration

curve. Select the method for the

determination of the calibration line. The following variants can be selected:

- Measure standard solutions

- Enter value pairs

- Enter formula

3 Using [Next], accept all entries on

the page and switch to the next page.

Note

During the following proceeding, you can return to the previous page at any time with [Back], e. g. if you want to correct entries, add further value pairs or eliminate outliers.

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Operation photoLab®6100 VIS

Edit method

04/16/07 9:52

Standard ID

Standard manufacturer

Back Next

Edit method

04/16/07 9:52

Tar g e t v alu e Ab s o r b a n c e E0 0.000 1

Back Add Delete Next

Edit method

04/16/07 9:52

Tar g e t v alu e Ab s o r b a n c e E0 0.000 1 0.300 2 0.600 3 1.000

Back Add Delete Next

Edit method

04/16/07 9:52

Tar g e t v alu e Ab s o r b a n c e E0 0.000 1 0.300 2 0.600 3 1.000

Back Add Delete Next

Variant 1:

Measure standard

solutions

1 Select and confirm Measure

standard solutions.

2 Enter and confirm details of the

standard solutions (optional).

3 Using [Next], accept all entries on

the page and switch to the next page.

The table for the measurement of standard solutions pops up.

In the first two lines of the table, the two value pairs (measuring points) that are at least required for a calibration are already prepared (reagent blank value E0 and any further nominal value).

4 Create further values pairs with

[Add] as necessary.

You can delete a highlighted value pair with [Delete].

5 In the Target value column, enter

the nominal values of the individual standard solutions.

Measuring the standard solutions:

6 Using the arrow keys <▲><▼>

and <W><X>, navigate to the relevant input field in the Absorbance column and press

<START·ENTER>.

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photoLab®6100 VIS Operation

Absorbance E0

04/16/07 9:52

To start measurement, insert cell or press <Start/Enter>

525 nm

16 mm

Absorbance E0

04/16/07 9:52

Last measured absorbance

0.009

Median

0.009 (1 Measurement(s))

525 nm

16 mm

Next meas.

Discard Apply

Edit method

04/16/07 9:52

Tar g e t v alu e Ab s o r b a n c e E0 0.000 0.009 1 0.300 0.664 2 0.600 1.292 3 1.000 2.178

Back Add Delete Next

The measurement display appears.

7 Insert the cell with the respective

standard.

The absorbance is measured. The result of the first single measurement is displayed.

8 If necessary, carry out further

single measurements for the formation of the median with [Next

meas.]

or discard the last single measurement with [Discard].

9 To accept the median value, press

[Apply].

Note

If the zero standard concentration (reagent blank value E0) is not measured and stored, the photometer calculates the calibration line without this value. If the User-defined blank value function (in the Concentration / Setup menu) is activated for measuring with this method, the value for a0 is determined and replaces the calculated axis intercept from the programming of the method.

10 Repeat the steps 6 to 9 until all

input fields in the Absorbance column are filled out.

11 Using [Next], accept all entries on

the page and switch to the next page.

The value pairs are displayed in a diagram (standard: Polygon line).

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Operation photoLab®6100 VIS

Edit method

04/16/07 9:52

f(x)=0.73x R2 = 1.000

Meas. range: 0.050 - 1.000 mg/l

Concentration [mg/l N O2-N]

Back Curve type Mea s. range Next

Absorbance

Edit method

04/16/07 9:52

Timer 0 00:00:00 Timer 1 00:00:00

Timer 2 00:00:00

Timer 3 00:00:00 AQA2 target value 1.00 mg/l AQA2 tolerance 0.10 mg/l

Back Complete

The related formula f(x) and correlation coefficient R

are

displayed above the diagram.

12 If required, select a different line

type for the line adjustment with [Curve type].

- Polygon line

- Straight line

- Parabola

13 If required, enter different

measured value limits with [Meas. range].

- Lower limit

- Upper limit

14 Using [Next], complete the editing

of the calibration line and proceed to the next page.

The timers and AQA2 data linked to the method are displayed.

Variant 2:

Enter value pairs

15 If necessary, enter intervals for up

to 4 timers.

16 If necessary, enter the AQA2

target value and AQA2 tolerance.

17 Complete the programming of the

method with [Complete].

The method is programmed and selected for measuring.

Unlike variant 1, the fields of the Absorbance column are filled out manually here. Accordingly, the steps 6 to 10 are not applicable here. Apart from that, the proceeding is identical to variant 1.

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photoLab®6100 VIS Operation

Edit method

16.04.07 9:52

c = a0 + a1·A + a2·A2 + a3·A3 + a4·A4 + a5·A

a0 0.605

a1 2 a2 a3 a4 a5 Lower limit of measuring range 1,000 mg/l Upper limit of measuring range 3.000 mg/l

Method list Delete Ne xt

Edit method

04/16/07 9:52

Timer 0 00:00:00 Timer 1 00:00:00

Timer 2 00:00:00

Timer 3 00:00:00 AQA2 target value 2.000 mg/l AQA2 tolerance 0.200 mg/l

Back Complete

Variant 3:

Enter formula

1 Select and confirm Enter formula.

Input fields for the coefficients (a0 ... a5) of the formula are displayed.

2 Enter and confirm the factors.

If no value is entered for a coefficient the photometer automatically uses the value 0.

Note

If a linear function (straight line) should be entered, it is necessary to enter the coefficients a0 and a1 to receive correct measured values. If the exact value for a0 is not known at the time the formula is entered, it is sufficient to enter the coefficient a1. In this case, the User-defined blank value function (in the Concentration / Setup menu) has to be activated to measure with this method. Prior to measuring with this method, a blank value measurement has to be carried out in this case. During this procedure the value for a0 is determined and replaces the previous value.

ba75847e01 08/2009

3 Enter and confirm the measuring

range limits.

4 Complete the entering of the

formula with [Next].

The timers and AQA2 data linked to the method are displayed.

5 If necessary, enter intervals for up

to 4 timers.

6 If necessary, enter the AQA2

target value and AQA2 tolerance.

7 Complete the programming of the

method with [Complete].

The method is programmed and selected for measuring.

Operation photoLab®6100 VIS

4.5.12 The IQ LabLink procedure

The IQ-LabLink procedure enables the secure data exchange between the sensors of the IQ S

ENSOR NET online measuring system and a photometer

(such as the spectroFlex series) with the aid of a commercial USB memory device.

During the matrix adjustment of the sensor, the measurement data of the IQ S

ENSOR NET sensor are adjusted to the photometrically determined

reference data. Previously, the reference data had to be entered manually during the matrix adjustment. With the aid of the IQ-LabLink procedure, the reference data can now be directly transferred to the IQ S

ENSOR NET sensors by means of a

USB memory device, without manual input and with no risk of confusion.

System requirement

for the

IQ-LabLink

procedure

Course of the

IQ-LabLink

procedure

/ IQ SENSOR NET:

– Terminal/controller with USB-A interface and software for the IQ-

LabLink procedure (e.g. MIQ/TC 2020 XT)

– Online sensor with software for the IQ-LabLink procedure

(e.g. VARiON

®Plus

700 IQ)

z Photometer:

– Photometer with software for the IQ-LabLink procedure

(e.g. spectroFlex series)

Step 1 on the IQ S

ENSOR NET terminal:

Automatic creation of a job file on the USB memory device with current sensor values, parameters, designation of the measuring location and automatic allocation of a job reference number for clear identification

Step 2 on the photometer:

Automatic recognition of the job files, menu-guided measurement of all required parameters, storage of the determined data in the job file

Step 3 on the IQ S

ENSOR NET terminal:

Automatic recognition of the job files, complete reading of all data required for the matrix adjustment on keypressing

Note

Carrying out the matrix adjustment of an online sensor with the IQ-LabLink procedure requires operating steps on both instruments: IQ S

ENSOR NET

system and spectroFlex photometer. The detailed description of the cross instrument operating steps for the matrix adjustment with the IQ-LabLink procedure on the IQ S

ENSOR NET and the

photometer is given in an additional operating manual. This operating manual can be downloaded from the Internet under www.wtw.com.

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photoLab®6100 VIS Operation

<HOME>

Absorbance / % Transmission

Absorbance

04/16/07 9:52

To start measurement, insert cell or press <Start/Enter>

300 nm

Setup Wave len gt h Transmission Refer ence

inner turn-up lid

4.6 Measuring the Absorbance / % Transmission

4.6.1 General information

The absorbance or transmission respectively is measured without the use of any methods or profiles. All settings are configured during measurement.

Measuring against

the Reference

absorbance

The absorbance or transmission can alternatively be measured against the absorbance of the zero adjustment or against a Reference absorbance determined by yourself (see section 4.6.3 M R

EFERENCE ABSORBANCE).

EASURING AGAINST THE

4.6.2 Measuring the absorbance or transmission

The settings of the last measurement are active.

1 Using [Wavelength], change the

wavelength as necessary.

2 Using [Absorbance] <–>

[Transmission], you can switch

over between absorbance and transmission measurement.

ba75847e01 08/2009

3 If necessary, use or measure a

reference measurement with [Reference] (see section 4.6.3).

4 Depending on the type, insert the

cell as follows:

Round cell:

Insert the round cell in the round cell shaft so it touches the bottom.

If the inner turn-up lid is opened too wide, a message prompts you to close the inner turn-up lid.

Operation photoLab®6100 VIS

Absorbance

04/16/07 9:52

0.860

489 nm

10 mm

Setup Wave len gt h Transmission Refer ence

Transmission

04/16/07 9:52

13.8

489 nm

10 mm

Setup Wave len gt h

Absorbance

Referenc e

Rectangular cell:

Open the inner turn-up lid.

Insert the rectangular cell vertically so it touches the bottom and left edge of the cell shaft. The opaque sides of the rectangular cell must point to the front and back.

The photometer has an external light recognition. If there is too much external light, a message prompts you to close the cell shaft cover.

The photometer starts measuring automatically.

5 Using [Absorbance] <–>

[Transmission], switch over the display from Absorbance to Transmission or vice versa.

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photoLab®6100 VIS Operation

<HOME>

Absorbance / % Transmission

Absorbance

04/16/07 9:52

To start measurement, insert cell or press <Start/Enter>

489 nm

10 mm

Setup Wave len gt h Transmission Refer ence

4.6.3 Measuring against the Reference absorbance

Each time the photometer is switched on, the absorbance or transmission is measured against the absorbance of the zero adjustment as a basis. You can, however, also determine a Reference absorbance and use it as the basis.

The Reference absorbance refers to the adjusted wavelength. The measured value remains stored until

z the photometer is switched off

z the cell type is changed

z the wavelength is changed

z a new reference value is measured

z it is deleted manually ([Reference] / Delete).

z the Absorbance / % Transmission measuring mode is exited

Single and multiple

determination

Measuring the

Reference

absorbance

The Reference absorbance can be determined with single or multiple determination. With multiple determination, the mean value is calculated as the median from the individual measured values.

The settings of the last measurement are active.

1 Start the reference measurement

with [Reference].

If a value for the reference absorbance is already stored, it can be deleted or overwritten by a new reference measurement.

After the reference absorbance value has been deleted, the photometer measures against the absorbance of the zero adjustment.

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Operation photoLab®6100 VIS

Reference absorbance

04/16/07 9:52

To start measurement, insert cell or press <Start/Enter>

489 nm

10 mm

Reference absorbance

04/16/07 9:52

Last measured absorbance

0.232

Median

0.232 (1 Measurement(s))

489 nm

10 mm

Next meas.

Discard Apply

Absorbance

04/16/07 9:52

Reference::

To start measurement, insert cell or press <Start/Enter>

489 nm

10 mm

Setup Wave len gt h Transmission Refer ence

2 Insert the cell with the reference

sample.

The first single measurement for the Reference absorbance is carried out.

The following data is displayed as the result:

– The measured absorbance

from the (last) single measurement.

– The median from all single

measurements carried out up to now.

3 If necessary, carry out further

single measurements for the formation of the median with [Next

meas.]

or discard the last single measurement with [Discard].

4 To accept the median value, press

[Apply].

The photometer is ready to measure.

The reference absorbance is displayed in the top right corner during absorbance or transmission measurement.

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photoLab®6100 VIS Operation

(equation 1)

1010...22110

AaAaAaa

⋅++⋅+⋅+

(equation 2)

)nm260(A76,0)nm280(A55,1]ml/mg[c

Protein

⋅−⋅=

4.7 Multi wavelengths methods

4.7.1 Basic information on Multi wavelengths measurements

The multi wavelength function of the photoLab calculation of any parameters from the absorbance values of several wavelengths. When doing so, two to ten wavelengths can be used.

Calculation takes place based on the following equation:

6100 VIS enables free

⋅++⋅+⋅+

1010...22110

AbAbAbb

with:

R Result

a0 to a10; b0 to b10 Freely selectable coefficients

(entry range 0.000 to 1000,000)

Example: Protein

determination

according to

Warburg-Christian

A1 to A10 Measured absorbance at the individual

wavelengths 1 to 10

The wavelengths 1 to 10 can be freely selected within the measuring range.

Protein determination according to Warburg-Christian is based on measurement of the optical density (=absorbance) at 260 and 280 nm in a 10 mm cell. From this the protein content is calculated as follows:

To convert the basic equation (1) into equation (2), the coefficients are set to the following values:

a0 =0 b0 =1

a1 =1,55 b1 =0 a1 = A(280 nm)

A2 = -0,76 b2 =0 A2 = A(260 nm)

A3 =0 b3 =0 A3 = Not used

A4 =0 b4 =0 A4 = Not used

a5 =0 b5 =0 a5 = Not used

a6 =0 b6 =0 a6 = Not used

a7 =0 b7 =0 a7 = Not used

a8 =0 b8 =0 a8 = Not used

a9 =0 b9 =0 a9 = Not used

a10 =0 b10 =0 a10 = Not used

All absorbances are measured in one step and the result is calculated according to the equation.

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Operation photoLab®6100 VIS

<HOME>

Multi wavelengths

– [Setup]

– Edit method

Edit method (1 of 6)

04/16/07 9:52

Number 2001 Name PROT Ver sion 1. 0 Citation form Protein Unit mg/ml Resolut ion 0.1 Cell 10 mm

Method list Delete Ne xt

4.7.2 Programming / modifying Multi wavelengths methods

Note

For multi wavelength methods, the method numbers 2001 to 2050 can be used. In the delivery condition, one method is stored for demonstration purposes.

1 Page 1 of 6:

Enter the general method data here. The next available method number is already entered as the number.

You have the following options when filling out the input fields:

- Fill out all empty input fields one after the other

- Using [Method list], select an already existing method as a model, give it a new method number and adjust the entries

- Using [Method list], select an existing method in order to change it (without changing the number).

- You can delete the method completely with [Delete].

2 Using [Next], accept all entries on

the page and switch to the next page.

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photoLab®6100 VIS Operation

Edit method (2 of 6)

04/16/07 9:52

Wavelength 1 280 nm Wavelength 2 260 nm

Back Add Delete D Next

Edit method (3 of 6)

04/16/07 9:52

a0 + a1 * A1 + a2 * A2 + ... + a10 * A10

b0 + b1 * A1 + b2 * A2 + ... + b10 * A10

a0 0.000 a6 0.000 a1 1.550 a7 0.000 a2 -0.757 a8 0.000 a3 0.000 a9 0.000 a4 0.000 a10 0.000 a5

Back Next

Edit method (4 of 6)

04/16/07 9:52

a0 + a1 * A1 + a2 * A2 + ... + a10 * A10

b0 + b1 * A1 + b2 * A2 + ... + b10 * A10

b0 1.000 b6 0.000 b1 0.000 b7 0.000 b2 0.000 b8 0.000 b3 0.000 b9 0.000 b4 0.000 b10 0.000 b5

Back Next

Edit method (5 of 6)

04/16/07 9:52

Number: 2002 Name: PROT Ver sion: 1 .0 Citation form: Protein Unit: mg/ml Resolut ion: 0.001 Cell: 10 mm

Back D Next

3 Page 2 of 6:

Add another wavelength with [Add].

Delete the last wavelength with [Delete].

4 Using [Next], accept all entries on

the page and switch to the next page.

5 Page 3 of 6:

Enter the coefficients for the basic equation (more detailed information of the basic equation, see section 4.7.1).

6 Using [Next], accept all entries on

the page and switch to the next page.

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7 Page 4 of 6:

Enter the coefficients for the basic equation (more detailed information of the basic equation, see section 4.7.1).

8 Using [Next], accept all entries on

the page and switch to the next page.

9 Page 5 of 6:

The data is displayed once again as a summary.

Using [Back], you can correct wrong data on the previous pages.

Using [Next], accept all entries on the page and switch to the next page.

The method is programmed and selected.

Operation photoLab®6100 VIS

Edit method (6 of 6)

04/16/07 9:52

1.550 * A(280 nm) - 0.757 * A(260 nm)

1.000

Back Complete

10 Page 6 of 6:

The basic equation is displayed (for more information on the basic equation see section 4.7.1).

11 Complete the editing with

[Complete].

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photoLab®6100 VIS Operation

<HOME>

Multi wavelengths

– [Method list]

Select method (all)

04/16/07 9:52

2001 Protein Protein mmol/l

2002 DNA purity

Last used

Select method (last use d)

04/16/07 9:52

2001 Protein Protein mmol/l

All methods

Pro_

4.7.3 Selecting a Multi wavelengths method

To select a method for Multi wavelengths measurements, proceed as follows:

The list of methods is displayed. The methods are ordered according to the method number.

Select the method:

1 Select the required method with

<▲><▼>. The active selection is

displayed in reverse video.

Narrowing down the

method list

Search function

2 Accept the selection with

<START·ENTER>.

The photometer is ready to measure.

If the list is very long, you can narrow down the method list and thus make the search easier as follows:

z Using [Last used], you can restrict the method list to the ten methods last

used.

z With the search function you can search certain character strings in the

list. The search takes place as a full-text search of the entire list contents. Thus you can search for a method number or certain citation form.

Search for a character string:

Enter the character string to be searched for in the search window with <A...9>.

The list appearing below shows all hits containing the character string. The hit list is updated with each character that is entered.

ba75847e01 08/2009

Note

Note the case sensitivity when searching.

Operation photoLab®6100 VIS

<HOME>

Multi wavelengths

04/16/07 9:52

Please select method for measuring!

Setup

Method list

Transmission

Multi wavelengths

04/16/07 9:52

To start measurement, insert cell or press <Start/Enter>

2001:PROT

Protein

10 mm

Setup

Method list

Transmission

inner turn-up lid

4.7.4 Carrying out Multi wavelengths measurements

1 Select the required method with

[Method list] (see section 4.7.3).

The photometer is ready to measure after the method has been selected.

2 Depending on the type, insert the

cell as follows:

Round cell:

Insert the round cell in the round cell shaft so it touches the bottom.

If the inner turn-up lid is opened too wide, a message prompts you to close the inner turn-up lid.

ba75847e01 08/2009

photoLab®6100 VIS Operation

Multi wavelengths

04/16/07 9:52

Zero adjustment successful

2001:PROT 10 mm

Setup

Method list

Transmission

Multi wavelengths

04/16/07 9:52

To start measurement, insert cell or press <Start/Enter>

2001:PROT

Protein

10 mm

Setup

Method list

Transmission

Multi wavelengths

04/16/07 9:52

A(280 nm) = 2.220 A(260 nm) = 0.925

2.738

mg/ml

2002: PROT Protein

10 mm

Setup

Method list

Transmission

Rectangular cell:

Open the inner turn-up lid.

Insert the rectangular cell vertically so it touches the bottom and left edge of the cell shaft. The opaque sides of the rectangular cell must point to the front and back.

The photometer has an external light recognition. If there is too much external light, a message prompts you to close the cell shaft cover.

If necessary, carry out a zero measurement.

ba75847e01 08/2009

The photometer is ready to measure.

The photometer starts measuring automatically.

If necessary, switch over to transmission display (%) with [Transmission].

Operation photoLab®6100 VIS

4.8 Spectrum

4.8.1 General information

With the Spectrum function, the absorbance or Transmission in dependency of the wavelength is measured and recorded. The wavelength range can be freely selected within the measuring range of the photometer. The increment is 1 nm.

A spectrum is recorded without using any methods or profiles. All settings are configured during measurement.

Baseline A baseline has to be recorded before a spectrum is recorded. The baseline

has to cover at least the wavelength range of the spectrum to be recorded. Once the baseline is measured, it remains stored in the photometer until

z a new baseline is recorded

z the Spectrum mode is exited or the photometer is switched off

Settings You can record a spectrum with standard settings without opening the setting

window. The following settings are possible for a spectrum:

Input field Possible entries

Wavelength start 320* ... 1100 nm

Wavelength stop 320 ... 1100* nm

Mode Absorbance* or

Transmission

Smoothing Yes* or No

Scaling Auto* or Manual

Scaling: Auto* During measurement, the instrument adjusts the

axis scaling (minimum and maximum value of the axis) to the measured values. The entire curve is always visible.

Scaling:Manual

Y-axis min

The axis scaling (minimum and maximum value of the axis) is set manually.

Y-axis max

* default setting

Note

You can store the settings as profile with [Save] . You can load a stored profile with [Open]. Profiles have the file extension "*.profil".

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photoLab®6100 VIS Operation

<HOME>

Spectrum

04/16/07 9:52

Wavelength [nm]

Setup Open

Absorbance

Spectrum

You have to record a baseline first (<ZERO>). Adjustment of wavelength range under <General setup>.

Spectrum

04/16/07 9:52

Wavelength start 320 nm Wavelength stop 1100 nm Mode Absorbance Cursor All values Smoothing Yes Scaling Auto

Apply

Spectrum

04/16/07 9:52

Wavelength [nm]

Setup Open

Absorbance

Spectrum

You have to record a baseline first (<ZERO>). Adjustment of wavelength range under <General setup>.

4.8.2 Recording the Spectrum

A message containing operating instructions is displayed.

1 Open the setting menu with

[Setup].

2 Select the start and end point of

the spectrum to be recorded and the mode (Absorbance or Transmission).

3 Accept all entries with [Apply].

A message containing operating instructions is displayed.

ba75847e01 08/2009

Operation photoLab®6100 VIS

Spectrum

04/16/07 9:52

Wavelength [nm]

Absorbance

Spectrum

04/16/07 9:52

Wavelength [nm]

Setup Open

Absorbance

Spectrum

Start measurement with <Start/Enter>

inner turn-up lid

Recording the baseline:

4 Press the <ZERO·BLANK> key.

The photometer records the baseline.

5 Wait until the baseline is

completely recorded.

The photometer is ready to measure after the baseline has been recorded.

Recording the spectrum:

6 Depending on the type, insert the

cell as follows:

Round cell:

Insert the round cell in the round cell shaft so it touches the bottom.

7 Close the inner turn-up lid.

8 Start the measurement with

<START·ENTER>.

After the spectrum has been recorded, the following message appears: Recording of spectrum is

completed.

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photoLab®6100 VIS Operation

Spectrum

04/16/07 9:52

Wavelength [nm]

Setup Edit Zoom Open

Absorbance

Spectrum

04/16/07 9:52

Wavelength [nm]

Setup Edit Zoom Open

Absorbance

Cursor information

Rectangular cell:

Open the inner turn-up lid.

Insert the rectangular cell vertically so it touches the bottom and left edge of the cell shaft. The opaque sides of the rectangular cell must point to the front and back.

9 Close the cell shaft cover.

10 Start the measurement with

<START·ENTER>.

11 Wait until the spectrum is

completely recorded.

At the end of the recording the following message appears:

Recording of spectrum is completed.

12 Confirm the message with

<START·ENTER>.

The cursor appears at the absolute maximum of the spectrum.

13 You have the following options:

- Immediately edit the spectrum (see section 4.8.3)

-With <PRINT>, you can output the spectrum to a connected printer as a graphic.

- You can save the spectrum as a *.csv file with <STORE>. As the storage location, you can select the photometer (Internal DataB folder) or a USB memory device connected to the USB-A connection (USB memory). Stored spectra can be recalled and edited at any time (see section 4.8.3).

ba75847e01 08/2009

Operation photoLab®6100 VIS

<HOME>

Spectrum

– [Open]

Open (Internal DataB folder)

04/16/07

26.02.07 Holmium.csv

23.02.07 K2Cr2O7_340nm.csv

Location

Delete

Spectrum

04/16/07 9:52

Wavelength [nm]

Setup Edit Zoom Open

Absorbance

Cursor information

4.8.3 Loading/editing a spectrum

A spectrum can be edited immediately after measurement. Stored spectra can be loaded and edited as well.

The following tools are available for editing:

z Cursor function for incremental moving along the curve with indication of

the x and y values

z Zoom function to scale up a section

z Mathematical functions for various evaluating and calculating operations.

The functions are described from page 86.

Loading a stored

spectrum

Cursor

The list with the spectra stored in the exchange memory is displayed.

1 If necessary, you can select a

different memory location for the spectrum with [Location] (USB memory device at the USB-A connection).

2 Select the required spectrum.

The original view of the curve is displayed.

The cursor consists of a horizontal and vertical line that cross each other on a point of the curve. A box names the x and y values of the point of the curve.

Move the cursor along the x axis (wavelength) with <W><X>. You can scan and evaluate the curve point after point.

ba75847e01 08/2009

photoLab®6100 VIS Operation

Spectrum

04/16/07 9:52

Wavelength [nm]

Setup xy min

Absorbance

Spectrum

04/16/07 9:52

Wavelength [nm]

Setup xy min

Absorbance

Spectrum

04/16/07 9:52

Wavelength [nm]

Setup xy max

Absorbance

Spectrum

04/16/07 9:52

Wavelength [nm]

Setup Edit Zoom Open

Absorbance

Zoom

1 Press [Zoom].

The zoom window appears. The bottom left corner of the zoom window is marked by a small black square.

– You can return to the original

view of the spectrum with [Original] at any time.

2 Adjusting the zoom window:

– Define the bottom left corner of

the zoom window with <W><X> and <▲><▼>.

–Use [xy max] to mark the top

right corner of the zoom window (small black square).

– Define the top right corner of the

zoom window with <W><X> and <▲><▼>.

3 Scaling up the zoom window:

– Press the <START·ENTER>

key. The zoom window is scaled up on the entire diagram area.

Leaving the zoom view:

– You can return to the original

view of the spectrum with <ESC> at any time.

ba75847e01 08/2009

Operation photoLab®6100 VIS

Edit Open the selection of mathematical functions with [Edit]:

z Extreme values (zoomed area)

Highlights the extreme values (minimum and maximum values) of the displayed spectrum.

z Mark points

Opens an edit mode where you can highlight individual points of the spectrum. With the [Mark] function key you can highlight individual points. The wavelength and measured valued are displayed at the highlighted point. With the [Delete] function key you can remove individual points.

z Delete all marks

Erases all highlighted points in the spectrum.

z Original

Displays the original, unedited spectrum.

z Integral

Calculates the area between the zero line and curve within a freely selectable wavelength interval [X1,X2].

z Derivative

Calculates the derivative of the total spectrum. To calculate the second and third derivative, the function can be carried out several times.

z Compare spectrum

Loads a second spectrum into the same diagram for direct comparison.

z Add spectrum

Adds a stored spectrum to the current spectrum.

z Subtract spectrum

Subtracts a stored spectrum from the current spectrum.

z Divide spectrum (ratio)

Divides the absorbance or % transmission values of the current spectrum by the values of a stored spectrum

z Add fixed value

Adds a constant absorbance or % transmission value to the current spectrum.

z Multiply fixed value

Multiplies the absorbance or % transmission values of the current spectrum by a constant value.

Note

The addition, subtraction and division of two spectra always applies to the common wavelength range of both spectra only.

ba75847e01 08/2009

photoLab®6100 VIS Operation

Spectrum

04/16/07 9:52

Wavelength [nm]

Setup Edit Zoom Open

Absorbance

4.8.4 Saving / exporting a spectrum

The saving of a spectrum saves both the edited and the original spectrum. Consequently, the original spectrum can be restored from each stored spectrum.

Saving

1 Record a spectrum (see section

4.8.2)

or Load a stored spectrum (see section 4.8.3).

2 If necessary, connect a USB

memory device to the USB-A interface.

3 Open the save dialog with

<STORE>.

4 If necessary, change the storage

location with [Location]: Internal DataB folder:

Exchange folder in the instrument or USB memory: USB memory device connected at the USB-A connection.

5 If necessary, change the file

name.

6 Save the file with

<START·ENTER>.

Export to a PC Export a stored spectrum to a PC: see section 4.12.3

ba75847e01 08/2009

Operation photoLab®6100 VIS

4.9 Kinetics

The Kinetics function enables the temporal tracing of the absorbance or transmission of a sample at a certain wavelength.

The photometer automatically calculates the slope between two adjacent measuring points from the available measurement data. The catalytic activity can also be determined and displayed if required.

To record the kinetics, the photometer carries out single measurements at regular intervals (measuring interval) and stores the measured values as a time function.

All settings for a recording are administrated as a profile. Profiles can be created, stored, edited and deleted. Each measurement requires a respective profile.

4.9.1 Creating/editing profiles for kinetic records

Note

Profiles for kinetic records are stored under the numbers 4001 to 4020. In the delivery condition, a profile is stored for demonstration purposes.

A profile for a kinetic recording comprises the following data:

Input field Possible entries

Number * 4001 ... 4020

Name Any name (max. 18 characters)

Mode* Absorbance or Transmission

Wavelength* Freely selectable (in nm)

Duration* Total duration in the format hh:mm:ss

(hours:minutes:seconds)

Interval* Measuring interval = time interval between two

successive single measurements in the format hh:mm:ss (hours:minutes:seconds)

Exception: With the setting, Measurements/interval: Max/ interval the interval is defined differently (see below).

Delay Time between the start of the recording and the

start of the first single measurement

Scaling Auto or Manual

ba75847e01 08/2009

photoLab®6100 VIS Operation

Cat. A. = mean value Slope [Δ/min] * Factor

Input field Possible entries

Scaling: Auto** During measurement, the instrument adjusts the

axis scaling (minimum and maximum value of the axis) to the measured values. The entire curve is always visible.

Scaling:Manual

Y-axis min

The axis scaling (minimum and maximum value of the axis) is set manually.

Y-axis max

Measurements/interval 1/interval or Max/interval

Here you define how many measurements are carried out per interval.

This setting has an impact on the calculation of the slope of the individual intervals (see section

4.9.6).

Catalytic activity Yes or No

Here you determine whether the catalytic activity should be calculated.

The catalytic activity is a measure for the amount of substance that is converted per time unit. To accelerate the substance conversion, a catalyst or enzyme (biological catalyst) is used in most cases.

Catalytic activity:Yes

Factor Unit

The catalytic activity or enzymatic activity is calculated from the slope of the curve.

Resolution

* necessary inputs ** default: Auto

ba75847e01 08/2009

Here you can enter the value for Factor.

The calculated value for the catalytic activity is displayed in the menu, [Edit] / Slope, together with the unit and resolution selected here.

Operation photoLab®6100 VIS

<HOME>

Kinetics

– [Setup]

– Edit profile

Edit profile (1 of 2)

04/16/07 9:52

Number 4001 Name NADH Mode Absorbance Wavelength 340 nm Duration 02:00:00 Interval 00:00:30 Delay 00:01:00 Scaling Auto

Profile Delete Next

Edit profile (1 of 2)

04/16/07 9:52

Measurements/interval

1/interval

Katalytic activity Yes Factor 1.000 Unit cat Resolut ion 0.01

Back Complete

Creating/editing a

profile

1 Enter the data for the profile here.

The next available profile number is already entered as the number.

You have the following options when filling out the input fields:

- Fill out all empty input fields one after the other

- Using [Profile], select an already existing profile as a model, give it a new profile number and adjust the entries

- Using [Profile], select an existing profile in order to change it (without changing the number).

- You can delete the profile completely with [Delete].

2 With [Next] you can switch to

further settings.

3 Enter further data for the profile

here.

4 Accept all entries with [Complete].

The profile is created and selected. The photometer is ready to measure.

Note

The Catalytic activity function is only available if the Absorbance mode was selected.

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photoLab®6100 VIS Operation

<HOME>

Kinetics

– [Profile]

Select profile (all)

04/16/07 9:52

4001 NADH Absorbance

4002 ADH Absorbance

Last used

Select profile (last used)

04/16/07 9:52

4001 NADH Absorbance

All profiles

NA_

4.9.2 Loading a profile for kinetic recording

To load a profile for kinetic recording, proceed as follows:

The list of profiles is displayed. The profiles are ordered according to the profile number.

Selecting a profile:

1 Select the required profile with

<▲><▼>. The active selection is

displayed in reverse video.

Narrowing down the

list of profiles

Search function

2 Accept the selection with

<START·ENTER>.

The photometer is ready to measure.

If the list is very long, you can narrow down the profile list and thus make the search easier as follows:

z Using [Last used], you can restrict the profile list to the ten profiles last

used.

z With the search function you can search certain character strings in the

list. The search takes place as a full-text search of the entire list contents. Thus you can search for a profile number or name.

Search for a character string:

Enter the character string to be searched for in the search window with <A...9>.

The list appearing below shows all hits containing the character string. The hit list is updated with each character that is entered.

ba75847e01 08/2009

Note

Note the case sensitivity when searching.

Operation photoLab®6100 VIS

<HOME>

Kinetics

04/16/07 9:52

Please select a profile for measuring!

Setup Profile Open

Kinetics

04/16/07 9:52

To start measurement, insert cell or press <Start/Enter>

4002

Absorbance

Setup Profile Open

inner turn-up lid

4.9.3 Recording the Kinetics

Note

During the recording, the photometer cannot carry out any regular self-test or self-calibration (AutoCheck), because the recording would have to be interrupted for this. A warm-up time of at least two hours is required for the photometer to measure reliably during the recording.

Note the warm-up time of at least 2 hours for kinetic recordings.

1 Select the required profile with

[Profile] (see section 4.9.2).

The photometer is ready to measure after the profile has been selected.

2 Depending on the type, insert the

cell as follows:

Round cell:

Insert the round cell in the round cell shaft so it touches the bottom.

If the inner turn-up lid is opened too wide, a message prompts you to close the inner turn-up lid.

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photoLab®6100 VIS Operation

Kinetics

04/16/07 9:52

4002 Number of measurements: 4 Duration: 00:00:24 Interval:00:00:06

Time [s]

Stop

Absorbance

Rectangular cell:

Open the inner turn-up lid.

Insert the rectangular cell vertically so it touches the bottom and left edge of the cell shaft. The opaque sides of the rectangular cell must point to the front and back.

The photometer has an external light recognition. If there is too much external light, a message prompts you to close the cell shaft cover.

The photometer starts recording automatically.

3 Wait until the recording is finished.

Stopping the recording:

-Use [Stop] to terminate the recording prematurely. The curve recorded up to this point can be stored and edited (see section 4.9.6).

-Use <ESC> to completely cancel measurement. The curve recorded up to this point is discarded.

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Operation photoLab®6100 VIS

Kinetics

04/16/07 9:52

4002 Number of measurements: 4 Duration: 00:00:24 Interval:00:00:06

Time [s]

Setup Profile Edit Open

Absorbance

Cursor information

4 After the specified Duration has

expired, the cursor appears.

You have the following options:

- You can move the cursor along the curve and have the measurement data for each point displayed (see section

4.9.6)

-With <PRINT>, you can output the kinetic curve to a connected printer as a graphic.

- You can store the kinetic curve with <STORE> (see section

4.9.4).

- Execute further functions to edit the kinetic record (see section

4.9.6)

- Close the kinetic record with

<ESC>.

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photoLab®6100 VIS Operation

Kinetics

04/16/07 9:52

4002 Number of measurements: 4 Duration: 00:00:24 Interval:00:00:06

Time [s]

Setup Profile Edit Open

Absorbance

4.9.4 Saving / exporting a kinetic record

Saving

1 Carry out the kinetic recording

(see section 4.9.3) or Load a stored kinetic record (see section 4.9.4).

2 If necessary, connect a USB

memory device to the USB-A interface.

3 Open the save dialog with

<STORE>.

4 If necessary, change the storage

location with [Location]: Internal DataB folder:

Exchange folder in the instrument or USB memory: USB memory device connected at the USB-A connection.

5 If necessary, change the file

name.

Export to a PC Export a stored kinetic record to a PC: see section 4.12.3

6 Save the file with

<START·ENTER>.

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Operation photoLab®6100 VIS

<HOME>

Kinetics

– [Open]

04/16/07

26.02.07 Enzyme kinetics.csv

24.02.07 ADH.csv

24.02.07 kinetics_4002_070224_1410.csv

Location

Delete

Kinetics

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4002 Number of measurements: 4 Duration: 00:00:24 Interval:00:00:06

Time [s]

Setup Edit Open

Absorbance

4.9.5 Loading a kinetic record

You can load and view stored kinetic records.

Loading a stored

Kinetics record

The list with the stored kinetic records is displayed (Internal DataB folder).

1 With [Location] select the memory

location of the kinetic record (Internal DataB folder or USB memory for a USB memory device at the USB-A connection).

2 Select the required kinetic record.

The curve is loaded.

You have the following options:

- You can move the cursor along the curve and have the measurement data for each point displayed (see section

4.9.6)

-With <PRINT>, you can output the kinetic curve to a connected printer as a graphic.

- You can store the kinetic curve with <STORE> (see section

4.9.4).

- Execute further functions to edit the kinetic record (see section

4.9.6)

- Close the kinetic record with

<ESC>.

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photoLab®6100 VIS Operation

Kinetics

04/16/07 9:52

4002 Number of measurements: 4 Duration: 00:00:24 Interval:00:00:06

Time [s]

Setup Edit Open

Absorbance

Cursor information

Kinetics

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0.63

cat

Interval Slop e (Δ/min) Time 1 0.000 5 s 2 0.000 10 s 3 0.000 15 s 4 0.000 20 s

5 0.000 25 s

6 0.000 30 s

Back

4.9.6 Editing a kinetic record

The following functions are available for kinetic records:

z Moving along the curve with the cursor

z Displaying a list with the slopes of the curve for each interval

z Scaling the Y-axis of the diagram

z Combined display of two kinetic records in one graphic

z Display of the difference of two kinetic records

Cursor

Slope of the curve &

catalytic activity

The cursor consists of a horizontal and vertical line that cross each other on a point of the curve. A box names the x and y values of the point of the curve.

Move the cursor along the x axis (time axis) with <W><X>. You can scan and evaluate the curve point after point.

The function, Slope indicates the slope of the kinetic curve in the individual intercepts (intervals) of the curve. An intercept corresponds to the Interval entered in the profile.

1 Indicate the slope of the kinetic

curve in the individual intercepts (intervals) of the curve with [Edit] / Slope.

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If the calculation of the catalytic activity was selected when the profile was created it is displayed here together with the slope.

Operation photoLab®6100 VIS

Note

The Slope function is only available if the kinetic recording was done in the Absorbance mode.

The displayed slope for an interval is determined as follows, depending on the slope:

Measurements/interval Slope

1/interval Slope,

converted to the interval, "1 minute"

Max/interval Slope of the straight line determined by linear

regression in an interval, converted to the interval, "1 minute"

Scaling of the Y-axis You can manually determine the scaling of the Y-axis with [Setup]/Scaling/

Manual.

Compare kinetics For direct comparison, you can load a second kinetic record into the same

diagram with [Edit] / Compare kinetics.

Note

The Compare kinetics function can only be carried out if both kinetic records were made in the Absorbance mode.

Subtract kinetics You can subtract a stored kinetic record from the current kinetic record with

[Edit] / Subtract kinetics.

Note

The Subtract kinetics can only be carried out if both kinetic records were made with the following settings:

z Mode: Absorbance

z Measurements/interval:1/interval

z Equal interval

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photoLab®6100 VIS Operation

4.10 Timer

You can use the timers to remind you by an acoustic signal of a time interval that has expired.

The photometer has two types of timers:

z The User defined timer is a timer that can be freely assigned. The interval

and name can be freely set. Only one freely assignable timer is available. It cannot be erased (see section 4.10.1).

z Analysis timer are timers permanently stored in the photometer. The

names and intervals of the analysis timers are stored in the method data of a measuring method (Concentration mode). The number of available analysis timers corresponds to the number of reaction times prescribed in the analysis instructions of the programmed methods (see section 4.10.2).

The photometer administrates all timers in the timer overview.

The timer overview (the Timer menu) is opened with the <TIMER> key. The Timer menu can be opened in any operating situation. Operation of the timer does not disturb any other functions. The timer overview can be exited with the <ESC> key.

When the Timer menu is opened for the first time, only the user-defined timer is in the timer overview. You can include analysis timers into the list or remove them according to your requirements (see section 4.10.2).

The timer overview displays the status of each timer and, of a started timer, the remaining time of the specified time interval.

All timers are started manually. As soon as one single timer has been started the timer symbol appears on the display in all operating modes.

When a timer has been started it is given the timer status, Active. When the specified time interval has expired the timer status changes from Active to Expired and an acoustic signal sounds. In the timer status Expired the acoustic signal sounds until the timer is stopped manually. After the stop, the timer status changes to Inactive and the acoustic signal is switched off.

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Operation photoLab®6100 VIS

<TIMER>

Timer

04/16/07 9:52

Designation Time Status

User defined timer 00:15:00 Inactiv

A6/25 - 1 00:15:00 Inactiv

Start Stop Edit Add

4.10.1 User defined timer

If you want to manually enter time intervals, use the User defined timer function.

The Timer menu is open.

1 Highlight the User defined timer.

2 If necessary, change the name

and time of the timer with [Edit].

3 Start the highlighted timer with

[Start].

The status of the timer is Active. When the specified time interval has expired, an acoustic signal sounds and the status changes to Expired.

4 Stop the highlighted timer with

[Stop].

The status of the timer changes to Inactive. The acoustic signal is switched off.

4.10.2 Analysis timer

Between the individual steps of a measurement, reaction times often have to be observed. The length of the reaction time is defined in the relevant analysis instructions.

For all required reaction times, the analysis timers with the corresponding time intervals are stored in the instrument. The names of the analysis timers include the method name and a current number so several timers within a method can be distinguished from each other.

To be able to use an analysis timer for a method you have to load it in the timer overview first. To do so, first select the required method and then add the available analysis timers to the timer overview so they can be started as necessary. The timer overview always comprises the free timer and the selected analysis timers.

100

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Xylem 6100-VIS User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

1 Overview

1.1 Overview of the instrument

1.2 Keypad

1.3 Display

2 Safety instructions

2.1 Target group and user qualification

2.2 Authorized use

2.3 General safety instructions

2.4 Handling of hazardous substances

3 Commissioning

3.1 Scope of delivery

3.2 Initial commissioning

3.2.1 Inserting the buffer batteries

3.2.2 Connecting the power supply

3.2.3 Switching on the photometer for the first time

3.2.4 Setting the language

3.2.5 Setting the date and time

3.3 Connecting optional accessories

3.3.1 Communication interfaces

3.3.2 PC/printer

3.3.3 USB memory device

3.3.4 PC keyboard

3.3.5 Barcode reader

4 Operation

4.1 Switching on or off the photometer

4.2 General operating principles

4.2.1 Navigating with function keys and menus

4.2.2 Display of navigation paths in short form

4.2.3 Entry of numerals, letters and characters

4.2.4 Detailed operating example: Changing the language

4.3 Photometer settings and system administration

4.3.1 Language

4.3.2 Date/Time

4.3.3 Display settings

4.4 Zero adjustment

4.5 Measuring in Concentration mode

4.5.1 Measuring cell tests with barcode

4.5.2 Measuring reagent tests with AutoSelector

4.5.3 Measuring reagent-free tests and user-defined methods

4.5.4 Exceeding the upper or lower limits of the measuring range

4.5.5 Selecting a method manually

4.5.6 Settings for Concentration mode

4.5.7 Measuring diluted samples

4.5.8 Sample blank value

4.5.9 Reagent blank value

4.5.10 Automatic Turbidity correction

4.5.11 Programming / modifying user-defined methods

4.5.12 The IQ LabLink procedure

4.6 Measuring the Absorbance / % Transmission

4.6.1 General information

4.6.2 Measuring the absorbance or transmission

4.6.3 Measuring against the Reference absorbance

4.7 Multi wavelengths methods

4.7.1 Basic information on Multi wavelengths measurements

4.7.2 Programming / modifying Multi wavelengths methods

4.7.3 Selecting a Multi wavelengths method

4.7.4 Carrying out Multi wavelengths measurements

4.8 Spectrum

4.8.1 General information

4.8.2 Recording the Spectrum

4.8.3 Loading/editing a spectrum

4.8.4 Saving / exporting a spectrum

4.9 Kinetics

4.9.1 Creating/editing profiles for kinetic records

4.9.2 Loading a profile for kinetic recording

4.9.3 Recording the Kinetics

4.9.4 Saving / exporting a kinetic record

4.9.5 Loading a kinetic record

4.9.6 Editing a kinetic record

4.10 Timer

4.10.1 User defined timer

4.10.2 Analysis timer