[Care and Use Manual 
]
Ultrahydrogel columns
i. Introduction
The Waters Ultrahydrogel™ column you have purchased is a sophisticated aqueous-compatible gel column for analytical and preparative separations of water-soluble polymers. The gel is a cross-linked hydroxylated polymer and contains some residual carboxyl functionality. Ultrahydrogel DP contains some residual amine functionality. Compounds normally characterized include oligomers and biological substances such as polysaccharides, nucleic acids, proteins, and peptides.
This Ultrahydrogel column provides:
•High resolution
•Low adsorption
•A wide range of molecular weight separations
•Stability in a pH range of 2 to 12
•A temperature range of 10 °C to 80 °C
Please take a few moments to read this manual carefully. Use the procedures it contains to ensure that you obtain quality results and take full advantage of the features your Waters column offers.
Contents
I. introduction
II. Operating tips
III. column efficiency Iv. column storage V. warranty
Vi. ordering information
Ultrahydrogel Columns |
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[Care and Use Manual 
]
II. Installation
a. Solvents
Ultrahydrogel columns are packed in distilled water for shipping. Table 1 shows the maximum solvent replace¬ment flow rate for each Ultrahydrogel column. Perform solvent changeover at a lower flow rate than that shown in Table 2 for analysis.
CAUTION: ULTRAHYDROGEL COLUMNS ARE FLOW (PRESSURE) SENSITIVE AND EXTREME CARE MUST BE TAKEN NOT TO EXCEED MAXIMUM FLOW RATE ON START-UP.
b. Column
Table 1. Flow Rates for Replacing Solvents
Column |
Column Size |
Max. Flow Rate During |
|
|
Solvent Change |
Ultrahydrogel (all pore sizes) |
7.8 mm i.d. x 30 cm |
0.3 ml/min |
|
|
|
Ultrahydrogel DP |
7.8 mm i.d. x 30 cm |
0.3 ml/min |
|
|
|
Ultrahydrogel DNA |
7.8 mm i.d. x 30 cm |
0.3 ml/min |
|
|
|
Ultrahydrogel Linear |
7.8 mm i.d. x 30 cm |
0.3 ml/min |
|
|
|
NOTE: CHANGE SOLVENTS AS SELDOM AS POSSIBLE. FREQUENT SOLVENT EXCHANGES ACCELERATE THE DEGRADATION OF COLUMN EFFICIENCY.
c. Organic Solvents
Ultrahydrogel columns are physically and chemically stable in ordinary water-soluble organic solvent mix¬tures. Aqueous solutions of methanol, ethanol, acetonitrile, formic acid, and dimethylsulfoxide also provide dependable separations. Organic solvent concentration should not exceed 20%. It is possible however, to use organic solvents up to 50% concentration by gradual introduction via gradient method.
d. Aqueous Salt and Buffer Solution
Although some non-ionic samples can be analyzed in distilled water, most water soluble polymers require aqueous salt or buffer solutions.
Some typical aqueous salt solutions:
•Sodium sulfate
•Sodium acetate
•Sodium dihydrogenphosphate
•Ammonium acetate
•Ammonium formate
Compatible buffer solutions:
•Phosphate
•Tris phosphate
•Tris hydrochloric acid
•Citrate
•Tris acetate
•Acetate
e. Flow Rate
For best resolution and maximum column life the flow rate should not exceed the maximum flow rate listed below. Use Table 2 below to determine recommended flow rates and maximum pressures.
Table 2. Recommended Flow Rates
Column |
Typical Operating Flow |
Maximum Flow Rate |
|
Rate (mL/min) |
(mL/min) |
|
|
|
Ultrahydrogel 120 |
0.5-0.8 |
1.0 |
|
|
|
Ultrahydrogel 250 |
0.5-0.8 |
1.0 |
|
|
|
Ultrahydrogel 500 |
0.3-0.6 |
1.0 |
|
|
|
Ultrahydrogel 1000 |
0.3-0.6 |
1.0 |
|
|
|
Ultrahydrogel 2000 |
0.3-0.6 |
1.0 |
|
|
|
Ultrahydrogel Linear |
0.3-0.6 |
1.0 |
|
|
|
Ultrahydrogel DP |
0.5-0.8 |
1.0 |
|
|
|
Ultrahydrogel Columns |
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