UVP Visi-Blue Transilluminators User Manual
Visi-Blue Transilluminators
Operating Instructions
IMPORT ANT: Please read these instructions before operating
your UVP Transilluminator to familiarize yourself with its operation.
Introduction
The Visi-Blue™ Transilluminators easily convert existing 365nm UV to 480nm blue light emitting no harmful UV
energy. Visi-Blue Transilluminators are designed for use with GelStar®, GFP, SYBR® green, Vistra Green,
SYPRO® Orange and Ethidium Bromide stains. As compared to UV , the blue light can transmit through almost
any transparent (plastic, glass, acrylic, etc.) laboratory equipment. This is essential when monitoring and detecting DNA migration and Protein expression in “real time.” When used in imaging applications, use the proper
photographic filter to eliminate/reduce the background. The amber colored protective cover blocks blue light
transmission and allows visualization of all media above 500nm.
Important Safety Information
A word of caution: Visi-Blue Transilluminators are equipped with ultraviolet tubes which are powerful sources of
365nm (blacklight/longwave) UV radiation. When inspecting or replacing 365nm UV tubes, it is IMPORT ANT that
UV Blocking Eyewear (faceshield recommended) is worn to avoid unportected eye and face exposure.
Dimensions and Specifications
The dimensions of the Visi-Blue Transilluminators are:
VB-26: Width 9.5” (241mm), Depth 13.25” (337mm), Height: 4.75” (121mm) Height includes cover
VB-40: Width 13.” (241mm), Depth 13.25” (337mm), Height: 5.63” (121mm) Height includes cover
Model Number length Volt/Hz/Amp Filter Size Tubes Style
VB-26 95-0326-01 480nm 115V/60Hz/0.70A 21 x 26 cm 4 x 8W Single
VB-26 95-0326-02 480nm 230V/50Hz/0.75A 21 x 26 cm 4 x 8W Single
VB-26 95-0326-03 480nm 100V/50-60Hz/0.80A 21 x 26 cm 4 x 8W Single
VB-40 95-0327-01 480nm 115V/60Hz/0.70A 20 x 40 cm 4 x 25W Single
VB-40 95-0327-02 480nm 230V/50Hz/0.75A 20 x 40 cm 4 x 25W Single
VB-40 95-0327-03 480nm 100V/50-60Hz/0.80A 20 x 40 cm 4 x 25W Single
Part Wave- Number of In te ns it y
Operation
1. Place the transilluminator on a level work surface. Be sure that an air space exists around the bottom of the
unit and the work surface. This space allows for the proper air circulation through the unit.
2. Plug the female end of the power cord into the transilluminator. For 230V models, or those requiring special
power cord connectors, ensure the proper configuration of male connector or plug has been properly connected to the power cord.
3. Plug the male end of the power cord into a properly grounded electrical outlet. The proper voltage of the
transilluminator is found on the product information label.
4. The Visi-Blue Transilluminator is equipped with an amber protective cover to protect the filter surface from
damage. The cover can be adjusted to various angles to suit the researcher’s needs. The cover is reversible
and can open away from the researcher by removing the four screws (two on both ends of the transilluminator)
and rotating the transilluminator top 180o.
5. Place gel/sample on the filter area. Gels may be placed on a UVP Gel-Tray (see Section 5.3 for accessory
part numbers) which protects the filter surface from cuts and scratches. It is recommended that gloves be
worn to avoid contact with gel and staining agents.
6. Press the ON/OFF switch to ON. The UV tubes within the unit should be glowing beneath the filter after a
momentary flickering during the start-up period.
7. After viewing/photographing the sample, turn the unit off.
Intensity of 365nm tubes without the Visi-Blue
conversion filter.
Visi-Blue conversion filter converts 365nm to
480nm providing high intensity output.
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