UVP Ultraviolet PCR User Manual

Ultraviolet PCR
Workstations and Cabinets

Installation and User Instructions

UVP, LLC Ultra-Violet Products Ltd.
2066 W 11th Street, Upland, CA 91786 Unit 1, Trinity Hall Farm Estate,
Tel: [phone] Nuffield Road Cambridge CB4 1TG UK
Fax: [phone] Tel: [phone] / Fax: [phone]

Web site: www.uvp.com

81-0228-01 Rev Y

Introduction

95-0367-01 115V

95-0367-03 100V

Light Sources:

 Chamber

Features include:

block below 400nm

Features include:

below 400nm

Features include:

block below 400nm

Features include:

below 400nm

Dimensions:

mm)

The Ultraviolet PCR Systems create an ideal environment for preparing PCR and other samples by
reducing the chance of contamination. The built-in high intensity UV provides a source for
inactivation of DNA between experiments. Additional contamination control is provided by the
uniquely coated stainless steel and aluminum design that maintains antimicrobial efficacy. The
surface resists growth of destructive bacteria, molds and fungi.

An optional UV/Air Circulator module connects to the interior of the workstation (95-0367-xx only) .
The module eliminates amplicons of DNA or genomic DNA from a previously dispensed
experiment. Connections for mounting and powering the module are built into the workstation. For
ordering information, refer to the “Maintenance, Replacement Parts/Accessories” section.

Safety Information

The Ultraviolet PCR Systems are designed with function, reliability, and safety in mind. Caution:
The unit includes shortwave UV, which is a powerful source of UV radiation that will cause damage
to unprotected eyes and skin. Before operating any unit, ensure all personnel in the area are
properly protected and that instructions for use of this equipment are followed. A safety shut-off
switch automatically turns the UV light off when the door is open, protecting users from UV
exposure. The Makrolon
32P labeled compounds.

®

Specifications

enclosure is UV and beta-blocking and effec tive for protection against

Ultraviolet PCR

Cabinet

95-0437-01 115V
95-0437-02 230V UK
95-0437-04 230V
Euro
95-0437-03 100V

Light Sources:

254nm 8-watt UV:

 Chamber

White 8-watt

 Chamber

Two power outlets
Two small shelves
UV Timer
Antimicrobial coated

stainless steel
(work surface) &
aluminum

®

Makrolon

panels

STANDARD UV PCR MODELS

UV2 PCR Cabinet

95-0436-01 115V
95-0436-02 230V UK
95-0436-04 230V
Euro
95-0436-03 100V

Light Sources:

254nm 8-watt UV:

 Chamber
 UV/air

circulator

White 8-watt:

 Chamber

Two power outlets
Two small shelves
UV Timer
Antimicrobial coated

stainless steel
(work surface) &
aluminum

Makrolon® panels block

Ultraviolet PCR

Workstation

95-0367-02 230V
UK
95-0367-04 230V
Euro

Light Sources:

254nm 25-watt UV:

 Chamber

White 15-watt:

 Chamber

Four power outlets
Two shelves
UV Timer
Antimicrobial coated

stainless steel
(work surface) &
aluminum

Makrolon® panels

UV2 PCR

Workstation

95-0439-01 115V
95-0439-02 230V UK
95-0439-04 230V
Euro
95-0439-03 100V

254nm 25-watt UV:

 Chamber

254nm 8-watt UV:

 UV/air

circulator

White 15-watt:

Four power outlets
Two shelves
UV Timer
Antimicrobial coated

stainless steel
(work surface) &
aluminum

Makrolon® panels block

Dimensions:

Exterior: 28.7H x

21.4W x 24D in.
(729 x 544 x 610 mm)
Interior: 19.7W x

21.4D in. (500 x 544
mm)

Exterior: 28.7H x

21.4W x 24D in.
(729 x 544 x 610
mm)
Interior: 19.7W x

21.4D in. (500 x 544

Dimensions:

Exterior: 28.7H x
29W x 24D in. (729
x 737 x 610 mm)
Interior: 27.8W x

21.4D in. (706 x 544
mm)

Dimensions:

Exterior: 28.7H x
29W x 24D in. (729 x
737 x 610 mm)
Interior: 27.8W x

21.4D in. (706 x 544
mm)

Ultraviolet PCR Cabinets and Workstations Page 3

Design

Formed antimicrobial coated aluminum, qty 2

Antimicrobial coated stainless steel (horizontal
work surface) and aluminum

Door and Side Panels

Two panel assembly, Makrolon®
115V

60 Hz

230V

50 Hz

100V

50/60 Hz

Part

Voltage

Max

95-0437-01

95-0439-05

115V

230V

9.1

8.7

Shelves

System Design

Interior
Exterior Aluminum powder coated

Electrical

Operational Ratings

• Unit intended to be used indoors

• Altitude must not exceed 2000 m

• Ambient temperature not to exceed 5°C to 40°C (41°F to 104°F)

• Relative humidity not to exceed 80% for temperatures up to 31°C (88°F) decreasing linearly to

50% relative humidity at 40°C (104°F)

• Mains supply voltage fluctuations up to ±10% of the nominal voltage

• Pollution rating 2 or better for laboratory areas

blocks wavelengths below 400nm

Number

95-0437-02
95-0437-04
95-0437-03
95-0437-05
95-0436-01
95-0436-02
95-0436-04
95-0436-03
95-0436-05
95-0367-01
95-0367-02
95-0367-04
95-0367-03
95-0367-05
95-0439-01
95-0439-02
95-0439-04
95-0439-03

230V
230V
100V
230V
115V
230V
230V
100V
230V
115V
230V
230V
100V
230V
115V
230V
230V
100V

Amps

8.8

8.8

9.1

8.8

9.1

8.7

8.7

9.1

8.7

8.8

8.6

8.6

8.8

8.6

9.1

8.7

8.7

9.1

Ultraviolet PCR Cabinets and Workstations Page 4

Screw

Screw

Base

Outer back panel. Note the notch in

Assembling the PCR System

Carefully remove the unit from the packaging materials. Follow the assembly instructions provided
in this manual. NOTE: Place the PCR base on a level surface large enough to accommodate the
entire base. The surface must be level to ensure proper ali gn ment of the Makrolon® panels.

Tools Required

• Philips head screwdriver

• Flathead screwdriver

• Gloves, to reduce transfer of fingerprints to PCR components

Two people are recommended for assembly or lifting of this equipment.
NOTE: Assembly drawings show the 95-0436-01 UV2 PCR Cabinet. These instructions apply for all PCR

models.

Outer Back Panel

NOTE: The outer and inner back panels and shelf bracket are assembled at the factory for
shipping purposes. The metal Makrolon® frames (2) are taped to the panels. Disassemble the
back panels/shelf bracket and remove the protective film prior to completing the assembly
instructions below.

1. Attach the outer back panel to the base with two screws as shown below.

Do not tighten the screws at this time.

the top of the panel allows room for

the wires.

Ultraviolet PCR Cabinets and Workstations Page 5

Inner back panel

Bracket

Position the inner

Cutout for wires

Inner Back Panel

1. Position the wires so that they run between the inner back panel and the outer back panel up to the
top for later connection to the top assembly.

2. Position the inner back panel so that the screw holes are aligned with the outer back panel. Attach
the inner back panel to the outer back panel with four (4) screws. Make sure the wires come out of
the cutout in the back panel, as shown by the red circle below.

3. Secure the shelf bracket in front of the inner back panel using:

a. Eight (8) screws in the Workstation or
b. Six (6) screws in the Cabinet

NOTE: Two people are required to hold the panels in place and insert the screws.
Do not fully tighten screws at this time.

panel so that the

edge of the frame

rests against the

stainless steel b ase