UVP Ultraviolet PCR User Manual

Ultraviolet PCR
Workstations and Cabinets

Installation and User Instructions

UVP, LLC Ultra-Violet Products Ltd.
2066 W 11th Street, Upland, CA 91786 Unit 1, Trinity Hall Farm Estate,
Tel: [phone] Nuffield Road Cambridge CB4 1TG UK
Fax: [phone] Tel: [phone] / Fax: [phone]

Web site: www.uvp.com

81-0228-01 Rev Y

Introduction

95-0367-01 115V

95-0367-03 100V

Light Sources:

 Chamber

Features include:

block below 400nm

Features include:

below 400nm

Features include:

block below 400nm

Features include:

below 400nm

Dimensions:

mm)

The Ultraviolet PCR Systems create an ideal environment for preparing PCR and other samples by
reducing the chance of contamination. The built-in high intensity UV provides a source for
inactivation of DNA between experiments. Additional contamination control is provided by the
uniquely coated stainless steel and aluminum design that maintains antimicrobial efficacy. The
surface resists growth of destructive bacteria, molds and fungi.

An optional UV/Air Circulator module connects to the interior of the workstation (95-0367-xx only) .
The module eliminates amplicons of DNA or genomic DNA from a previously dispensed
experiment. Connections for mounting and powering the module are built into the workstation. For
ordering information, refer to the “Maintenance, Replacement Parts/Accessories” section.

Safety Information

The Ultraviolet PCR Systems are designed with function, reliability, and safety in mind. Caution:
The unit includes shortwave UV, which is a powerful source of UV radiation that will cause damage
to unprotected eyes and skin. Before operating any unit, ensure all personnel in the area are
properly protected and that instructions for use of this equipment are followed. A safety shut-off
switch automatically turns the UV light off when the door is open, protecting users from UV
exposure. The Makrolon
32P labeled compounds.

®

Specifications

enclosure is UV and beta-blocking and effec tive for protection against

Ultraviolet PCR

Cabinet

95-0437-01 115V
95-0437-02 230V UK
95-0437-04 230V
Euro
95-0437-03 100V

Light Sources:

254nm 8-watt UV:

 Chamber

White 8-watt

 Chamber

Two power outlets
Two small shelves
UV Timer
Antimicrobial coated

stainless steel
(work surface) &
aluminum

®

Makrolon

panels

STANDARD UV PCR MODELS

UV2 PCR Cabinet

95-0436-01 115V
95-0436-02 230V UK
95-0436-04 230V
Euro
95-0436-03 100V

Light Sources:

254nm 8-watt UV:

 Chamber
 UV/air

circulator

White 8-watt:

 Chamber

Two power outlets
Two small shelves
UV Timer
Antimicrobial coated

stainless steel
(work surface) &
aluminum

Makrolon® panels block

Ultraviolet PCR

Workstation

95-0367-02 230V
UK
95-0367-04 230V
Euro

Light Sources:

254nm 25-watt UV:

 Chamber

White 15-watt:

 Chamber

Four power outlets
Two shelves
UV Timer
Antimicrobial coated

stainless steel
(work surface) &
aluminum

Makrolon® panels

UV2 PCR

Workstation

95-0439-01 115V
95-0439-02 230V UK
95-0439-04 230V
Euro
95-0439-03 100V

254nm 25-watt UV:

 Chamber

254nm 8-watt UV:

 UV/air

circulator

White 15-watt:

Four power outlets
Two shelves
UV Timer
Antimicrobial coated

stainless steel
(work surface) &
aluminum

Makrolon® panels block

Dimensions:

Exterior: 28.7H x

21.4W x 24D in.
(729 x 544 x 610 mm)
Interior: 19.7W x

21.4D in. (500 x 544
mm)

Exterior: 28.7H x

21.4W x 24D in.
(729 x 544 x 610
mm)
Interior: 19.7W x

21.4D in. (500 x 544

Dimensions:

Exterior: 28.7H x
29W x 24D in. (729
x 737 x 610 mm)
Interior: 27.8W x

21.4D in. (706 x 544
mm)

Dimensions:

Exterior: 28.7H x
29W x 24D in. (729 x
737 x 610 mm)
Interior: 27.8W x

21.4D in. (706 x 544
mm)

Ultraviolet PCR Cabinets and Workstations Page 3

Design

Formed antimicrobial coated aluminum, qty 2

Antimicrobial coated stainless steel (horizontal
work surface) and aluminum

Door and Side Panels

Two panel assembly, Makrolon®
115V

60 Hz

230V

50 Hz

100V

50/60 Hz

Part

Voltage

Max

95-0437-01

95-0439-05

115V

230V

9.1

8.7

Shelves

System Design

Interior
Exterior Aluminum powder coated

Electrical

Operational Ratings

• Unit intended to be used indoors

• Altitude must not exceed 2000 m

• Ambient temperature not to exceed 5°C to 40°C (41°F to 104°F)

• Relative humidity not to exceed 80% for temperatures up to 31°C (88°F) decreasing linearly to

50% relative humidity at 40°C (104°F)

• Mains supply voltage fluctuations up to ±10% of the nominal voltage

• Pollution rating 2 or better for laboratory areas

blocks wavelengths below 400nm

Number

95-0437-02
95-0437-04
95-0437-03
95-0437-05
95-0436-01
95-0436-02
95-0436-04
95-0436-03
95-0436-05
95-0367-01
95-0367-02
95-0367-04
95-0367-03
95-0367-05
95-0439-01
95-0439-02
95-0439-04
95-0439-03

230V
230V
100V
230V
115V
230V
230V
100V
230V
115V
230V
230V
100V
230V
115V
230V
230V
100V

Amps

8.8

8.8

9.1

8.8

9.1

8.7

8.7

9.1

8.7

8.8

8.6

8.6

8.8

8.6

9.1

8.7

8.7

9.1

Ultraviolet PCR Cabinets and Workstations Page 4

Screw

Screw

Base

Outer back panel. Note the notch in

Assembling the PCR System

Carefully remove the unit from the packaging materials. Follow the assembly instructions provided
in this manual. NOTE: Place the PCR base on a level surface large enough to accommodate the
entire base. The surface must be level to ensure proper ali gn ment of the Makrolon® panels.

Tools Required

• Philips head screwdriver

• Flathead screwdriver

• Gloves, to reduce transfer of fingerprints to PCR components

Two people are recommended for assembly or lifting of this equipment.
NOTE: Assembly drawings show the 95-0436-01 UV2 PCR Cabinet. These instructions apply for all PCR

models.

Outer Back Panel

NOTE: The outer and inner back panels and shelf bracket are assembled at the factory for
shipping purposes. The metal Makrolon® frames (2) are taped to the panels. Disassemble the
back panels/shelf bracket and remove the protective film prior to completing the assembly
instructions below.

1. Attach the outer back panel to the base with two screws as shown below.

Do not tighten the screws at this time.

the top of the panel allows room for

the wires.

Ultraviolet PCR Cabinets and Workstations Page 5

Inner back panel

Bracket

Position the inner

Cutout for wires

Inner Back Panel

1. Position the wires so that they run between the inner back panel and the outer back panel up to the
top for later connection to the top assembly.

2. Position the inner back panel so that the screw holes are aligned with the outer back panel. Attach
the inner back panel to the outer back panel with four (4) screws. Make sure the wires come out of
the cutout in the back panel, as shown by the red circle below.

3. Secure the shelf bracket in front of the inner back panel using:

a. Eight (8) screws in the Workstation or
b. Six (6) screws in the Cabinet

NOTE: Two people are required to hold the panels in place and insert the screws.
Do not fully tighten screws at this time.

panel so that the

edge of the frame

rests against the

stainless steel b ase

Ultraviolet PCR Cabinets and Workstations Page 6

Frames for

Makrolon® Panels

Shelf Bracket

Makrolon® Panel Assembly

1. Loosen the screws (4 per side) attaching the Makrolon® panel frames to the inner back panel.

2. Slide the frames for the Makrolon
frames are under the shelf brackets.

3. Remove the protective film from the Makrolon
brackets and the inner back panel.

4. Press the bracket s tightly against the Makrolon

5. Tighten all frame screws to secure the Makrolon

Makrolon® Panels

®

panels under the screws on the inner back assembl y s o th a t the

®

panels. Slide the panels into position between the

®

panels.

®

panels in place.

Ultraviolet PCR Cabinets and Workstations Page 7

WARNING: Ensure that the unit is not plugged in.

Flange

Screw locations

Shelf bracket

Top Assembly

Top Assembly and Shelf Assembly

1. Rest the top assembly on top of the unit.

2. Connect the bottom and top assembly wires together according to their labels, matching wire “A”
with wire “A,” wire “B” with wire “B” and so forth, until all labeled wires are connected. Connect the
wires by joining the male and female red connectors located on the ends of each wire. Then,
connect the two unlabeled green/yellow ground wires together using the red connectors.

3. Slide the top assembly down so that the Makrolon
Make sure that the back flange of the top assembly is positioned between the inner back and the
outer back panel. Slide the wires into the space between the inner and outer back panels. Secure
and tighten the top assembly to the back panel with screws, pushing from the back to ensure the
screws are secure.

4. Slide the shelves into the shelf bracket.

®

panels are inside the top assembly channels.

Ultraviolet PCR Cabinets and Workstations Page 8

Thumb Knobs

Door Assembly

Side Brackets

Magnet

Top Assembly

Door Hinges

Door Assembly

1. Slide the upper panel of the door assembly into slotted area in the top assembly. Holding the door
in position, insert the thumb screws (Qty. 3) inside the top assembly.

2. Secure the upper panel of the door assembly to the left and right side panels with the two side
brackets.

3. Create the door assembly by connecting the upper and lower door panels using the two hinges and
8 nuts.

4. A magnet is attached to the bottom right bracket on the door. The purpose of the magnet is to shut
off the overhead UV light when the door is open.

Note: Some systems may have a single large bracket running along the width of the entire lower
door, rather than the two small brackets shown below.

WARNING: The PCR is for laboratory use and cannot be connected to a public power supply.

When the PCR is assembled, plug the power cord into the back of the unit and then into a wall
outlet in the laboratory.

Operating the PCR Systems

The Ultraviolet PCR Systems feature:

• Overhead UV for decontamination of the chamber or PCR equipment

• UV safety switch and keylock for preventing exposure to UV

• Antimicrobial coated stainles s stee l and aluminum for reducing bacterial growth

• Easy-access door

• Overhead white light

• Shelves for placement of tools

• Built-in power outlets built into the chamber interior

Ultraviolet PCR Cabinets and Workstations Page 9

Power switch

Overhead
white light

UV keylock

115V

230V UK

230V Euro

30-minute

UV timer

Timer

Use of PCR Equipment inside the Cabinet or Workstation

Shelves are provided for placement of small tools for decontamination.
Power outlets allow use of shakers, rockers, and other equipment for PCR experiments inside the

PCR chamber. The equipment can be decontaminated between experiments.
Power plugs built into the chamber allow use of shakers, rockers, and other equipment for PCR

experiments. Equipment can be decontaminated between uses. To operate equipment within the
chamber, plug the equipment into the power plug. Specific power plugs are installed as shown in
the illustrations below.

NOTE: When operating equipment plugged into the outlets, combined Amps total is not to
exceed 8 Amps.

NOTE: Crazing is a normal process for Makrolon

over a period of time. Reduce crazing by keeping exposure to UV to a minimum. Crazing may
occur within the warranty period and is regarded as normal wear and tear not covered by the
warranty. Makrolon® panels can be replaced. Refer to Replacement Parks for ordering information.

Operating the UV and Fluorescent White Lights

UV Keylock

The Ultraviolet PCR Cabinets and Workstations provide an automated process for eliminating
contamination by utilizing the germicidal properties of shortwave 254nm ultraviolet light. The UV
light is typically operated when no samples are inside the chamber. The chamber is
decontaminated, eliminating viable fungi, bacteria, and yeast.

for the Optional

UV/Air

Circulator

To use the UV decontamination, turn the UV keylock ON. Then press the timer to operate the
germicidal lamp housed within the chamber. The UV timer allows selection of UV exposure time.
The timer may take a few seconds to initialize once the timer has power. The timer will only operate
when the door is shut and the magnetic safety switch on the door is engaged. When door is open
the timer will not operate.

®

panels exposed to UV light. Crazing will occur

calibration

button hole

Ultraviolet PCR Cabinets and Workstations Page 10

UV2 PCR Cabinet and Workstation. Airflow

The default timer setting is 30 minutes, adjustable at 5 minute increments from 5 to 60 minutes,
then at 15 minute increments up to 12 hours. To adjust the timer settings:

1. Turn the system on and wait until the timer reads “OFF”.

2. Use a small paper clip to press the Timer calibration button loc ated in t he small hole to
the left of the timer for one second.

3. Press the main timer button to adjust the timer settings. Each press will increase the
amount of time by 5 minutes (eg 35:00, 40:00, etc.) until one hour is reached. After one
hour is reached, the time will be adjusted in 15-minute increments and will read without a
colon. For example, one hour will read “1 00,” one hour and 15 minutes will read “1 15”
and so forth.

4. Once the desired time setting is reached, do not press any additional buttons. The timer
will then cycle through the remainder of its settings. When the timer reads “OFF” for 5
seconds without blinking, the timer is ready to use with the new time setting.

Turn the key to the off position after UV irradiation to prevent exposure of samples to the UV.
Ensure the key is completely inserted into the lock.

[Note] Do not attempt to perform PCR procedures with the germicidal lamp on. The germicidal
lamp is used to decontaminate the internal chamber of the between experiments.

[Warning] Exposure to UV light is harmful.
The Makrolon

germicidal lamp will shut off if the panel door is opened. The PCR Workstation contains a powerful
source of UV radiation that will cause damage to unprotected eyes and skin. Before operating any
unit, be sure all personnel in the area are properly protected. Even though the unit shuts the UV off
when the door is open, UV Blocking Eyewear should be worn as well. UVP has a complete line of
UV Blocking Eyewear: Spectacles, Goggles, and Faceshield designed for this purpose.

Extended periods of time UV exposure can be used to disinfect the interior chamber by the
destruction of organisms. Refer to the Maintenance section for information on measuring the
intensity of the UV tubes with a UV meter.

Overhead White Light

The overhead white light can remain lit at any time throughout experiment procedures or the
decontamination process. The fluorescent white light provides a bright light within the PCR
Workstation. To use the white light, press the ON button located on the top panel.

UV Air C irculator

The UV2 PCR Cabinet and UV2 PCR Workstation inclu de
a built-in UV Air Circulator.

To operate the circulator, press the UV/Air Circulator
switch to the ON position. The circulator will pull air from
inside the chamber through the circulator,
decontaminating the air with shortwave UV and blowing
the air back into the chamber.

NOTE: Do not place equipment in front of the air vents to
block air flow.

An optional UV/Air Circulator module can be mounted into
the top of workstation chamber (95-00367-XX only) for
elimination of amplicons of DNA or genomic DNA from a
previously dispensed experiment. The power switch,
outlet, and mounting brackets for the circulator are pre­installed at the factory if users purchase a module
at a later time.

®

door is UV blocking and will not allow UV radiation to pass through the door. The

into and out of the UV Air Circulator

Ultraviolet PCR Cabinets and Workstations Page 11

Microwatt

seconds/cm

Microwatt

seconds/cm2

Saccharomyces
ellipsoideus

B. parathyphosus

6100

Brewer’s yeast

6600

B. subtilis

11000

Baker’s yeast

8800

Corynebacterium diptheriae

6500

MOLD SPORES

Escherichlia coli

6600

Penicillium expansum

22000

Micrococcus cadidus

12300

Penicillium digitatus

88000

Micrococcus sphaeroides

15400

Aspergillus glaucus

88000

Proteus vulgaris

6600

Mucor racemosus A

35200

Pseudomonas aeruginosa

10500

Mucor racemosus B

35200

Salmonella

10000

VIRUS

Sarratia marcescens

6160

Tobacco mosaic

44000

Dysentery bacilli

4200

Influenza

6600

Shigella paradyseneriae

3200

PROTOZOA

Staphylococcus aereus

6600

Nematode eggs

9200

Streptococcus lactis

8800

Measuring the UV Tube Intensity

As the ultraviolet tubes age, the intensity and germicidal destruction rate decreases. It is important
to monitor the efficiency of the tubes to ensure the germicidal requirements are met. The germicidal
destruction rate is a function of the UV intensity at wavelength 254nm and exposure time. The
lower the tube intensity, the longer the lamps must be on to accomplish the same objective.

The UVX Radiometer using a 254nm sensor will allow the user to measure the 254nm emissions
from the tubes. When it is time to measure the UV intensity inside the PCR workstation
(recommended weekly or as needed), the sensors can be placed on the floor of the PCR. Close the
hood and turn on the UV tubes. Wait three minutes to record the measurement on the UV meter. It
is recommended that the user record an initial value upon receipt and assembly of the PCR unit.

The germicidal destruction rate calculation:

Microwatt seconds/cm2 = microwatts/cm2 x seconds of exposure

For ordering information on the UVX Radiometer, J-225 meter and sensors, refer to the
Replacement Parts and Accessories section in this manual.

The bacterial destruction chart below indicates the amount of shortwave (254nm) UV energy
required for complete destruction of various organisms.

Bacterial Organisms

Bacillus anthracis 8700
S. enteritidis 7600
B. Megatherium sp. (veg.) 2500 Saccharomyces sp. 17600

B. Megatherium sp. (spores) 5200 Saccharomyces cerevisiae 13200

Additional Organisms

2

YEAST

13200

B. subtilis spores 22000 Common yeast cake 13200
Clostridium tetani 22000

Eberthella typosa 4100 Penicillium roqueforti 26400

Mycobacterium tuberculosis 1000 Aspergillus flavus 99000
Neisseria catarrhalis 8500 Aspergillus niger 330000
Phytomonas tumefaciens 8500 Rhisopus nigricans 220000

Pseudomonas fluorescens 6600 Oospora lactis 11000
S. typhimusium 15200

Sarcina lutea 26400 Bacteriophasge (E. coli) 6600

Spirillum rubrum 6160
Staphlococcus albus 5720 Paramecium 200000

Streptococcus hemolyticus 5500 Chlorella vulgaris (algae) 22000

Streptococcus viridans 3800

Ultraviolet PCR Cabinets and Workstations Page 12

System/Part
Number

Replacement
Tube

Tube
Description

Qty.

UV PCR Cabinet

34-0007-01

8W 254nm UV

4

95-0437-xx

34-0056-01

8W White

2

UV2 PCR Cabinet

34-0007-01

8W 254nm UV

5

95-0436-xx

34-0056-01

8W White

2

Maintenance, Replacement Parts/Accessories

NOTE: Crazing is a normal process for Makrolon® panels exposed to UV light. Crazing will occur
over a period of time. Reduce crazing by keeping exposure to UV to a minimum. Crazing may
occur within the warranty period and is regarded as normal wear and not covered by the warranty.
Makrolon

Care and Cleaning

The door and side panels are made of Makrolon® and are subject to scuffing and scratches if
improperly cleaned. The stainless steel and aluminum surfaces are manufactured with an
antimicrobial coating to reduce bacterial growth. Care in cleaning and use of the equipment is
recommended to reduce wear of the coating. To clean the unit:

®

panels can be replaced. Refer to Replacement Parks for ordering information.

• Wipe excess water from inside the unit and outside the unit with an absorbent soft cloth or
sponge.

• Use mild soap and a damp soft cloth or damp sponge to clean the exterior of the unit.

• Use a mild detergent on the interior surfaces.

• Warning: Unplug the unit before cleaning around the UV tube contacts and internal plug

outlets.

• Clean the door with a mild detergent; never use organic based compounds, Alcohol, or
Ammonia containing cleaners.

• Do not use abrasive pads or cleansers.

• A plastic cleaner solution is recommended to clean the door and is available from local

plastic supply distributors.

Replacement Parts and Accessories

Replacement parts are listed below. Only authorized UVP service personnel should perform repairs
or replacements other than specified in these procedures.

Replacement Part Part Number

Key, replacement 53-0178-04
Shelf (for workstations) 20-0970-02
Shelf (for cabinets) 20-1209-01
Fuse (2 required) 56-0049-01

Makrolon

Side Panels (2 Required) 10-0618-01
Upper Door Panel (Workstation) 10-0619-01

Lower Door Panel (Workstation) 10-0620-01
Upper Door Panel (Cabinet) 10-0621-01

Lower Door Panel (Cabinet) 10-0622-01

Replacement Tubes

®

Panels

Ultraviolet PCR Cabinets and Workstations Page 13

UV PCR Workstation

34-0073-01

25W 254nm
UV

2

95-0367-xx

34-0087-01

15w White

1

UV2 PCR
Workstation

34-0007-01

8W 254nm UV

1

95-0439-xx

34-0073-01

25W 254nm
UV 2

34-0087-01

15w White

1

Accessories Part Number

Table, stainless steel top 98-0077-01
Shelf, long 27” 20-0970-01
UV/Air Circulator Module (for 95-0367-xx) 97-0157-01
UVX Radiometer 97-0015-02
UVX Sensor (UVX-25) 97-0016-01
J-225 Meter 97-0004-01
Spectacles, UV blocking (UVC-303) 98-0002-01
Goggles, UV blocking (UVC-503) 98-0002-02
Face Shield, UV blocking (UVC-803) 98-0002-04

PCR Table

Replacing the UV Germicidal or White Light Tubes

Turn the unit power off and unplug the unit from the outlet. Lift up the panel door and locate the
tubes. The tube has two prongs on each end that first fit into a socket then rotate into place.
Carefully hold the tube on the end and insert the tube into the socket.

Twist the tube 90° until the tube locks into place.

Cabinet:

Outer (White Bulb) – White light (8 watt)
Inner (Clear Bulb) – UV (8 watt)

Workstation:

Outer (Clear Bulb) – UV
Inner (White Bulb) – White light

Replacing the Fuses

Fuses are located on lower left side of the
system. To remove the fuse, use a flathead
screwdriver to turn the fuse counter
clockwise. Pull the fuse out. Insert the new
fuse and using the screwdriver, turn the fuse
clockwise to lock.

Ultraviolet PCR Cabinets and Workstations Page 14

p.m. PST.

5:30 p.m.

[e-mail]

Cambridge CB4 1TG UK

Circulator

Replacing the Tube in UV/Air Circulator

1. Locate and remove the two thumb knobs on
the circulator cap on the outer back panel of
the system.

2. Pull the circulator cap away from the system.

3. The tube, attached to the cap, has two prongs
on each end that fit into a socket. Carefully
hold the tube on the ends, rotate the tube and
remove from the cap. Insert a new tube into the
socket. Twist the tube 90° until the tube locks
into place.

4. Replace the place cap back into position and reattach the two thumb knobs.

cap with

UV tube

Technical Assistance

UVP offers technical support for all of its products. If you have any questions about the product’s
use, operation or repair, please contact our offices at the locations below.

Note: A Returned Goods Authorization (RGA) number must be obtained from UVP Customer
Service before returning any product.

You may contact UVP’s Customer Service based on your location:

If you are in North America, South
America, East Asia or Australia:

If you are in Europe, Africa, the
Middle East or Western Asia:

ask for Customer Service during regular
business days, between 7:00 a.m. and 5:00

E-Mail your message to: [e-mail]

Write to: UVP, LLC 2066 W. 11th Street,

Upland, CA 91786 USA

Warranty

UVP, LLC warrants its products to be free of detects in materials and workmanship for a period of
two (2) years from the date of purchase. The foregoing warranty of UVP shall be of no force and
effect if buyer has modified or damaged the product. Tube s and filters are warranted for 90 days.

NOTE: “Crazing” (cracking and/or yellowing) of the Makrolon
warranty period. This is regarded as normal wear and tear and is not covered by the warranty.

All warranties or merchantability and fitness for any purpose and all other warranties, expressed or
implied, except those expressly set forth herein, are deemed waived and excluded.

UVP’s duty under the warranty is limited to replacement and/or repair of the defective part at the
option of UVP, LLC. UVP shall not be liable for any expenses or damages incurred by the
purchaser except as expressly set forth herein, and in no event shall UVP be liable for any special,
incidental or consequential damages of any kind. This warranty does not supersede any statutory
rights that may be available in certain countries.

Makrolon is a registered trademark of Bayer AG.

Customer Service during regular
business days, between 8:30 a.m. and

Unit 1, Trinity Hall, Nuffield Road,

®

panels may occur within the