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NanoDrop Micro-UV/Vis Spectrophotometer
NanoDrop OneC with NanoDrop QC Software
User Guide
269-342200 NanoDrop QC UG Revision A December 2019
© 2019 Thermo Fisher Scientific Inc. All rights reserved.
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For U.S. Technical Support, please contact:
Thermo Fisher Scientific
3411 Silverside Road
Tatnall Building, Suite 100
Wilmington, DE 19810 U.S.A.
Telephone: 302 479 7707
Toll Free: 1 877 724 7690 (U.S. & Canada only) E-mail: nanodrop@thermofisher.com
For International Support, please contact:
http://www.nanodrop.com/support
Contact your local distributor. For contact information go to:
http://www.nanodrop.com/Order.aspx
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product operation. This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical information in this document is for reference purposes only. System configurations and specifications in this document supersede all previous information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or errorfree and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might result from any use of this document, even if the information in the document is followed properly.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all conflicting information between the two documents.
For Research Use Only. This instrument or accessory is not a medical device and is not intended to be used for the prevention, diagnosis, treatment or cure of disease.
WARNING Avoid an explosion or fire hazard. This instrument or accessory is not designed for use in an explosive atmosphere.
C
Contents
Chapter 1 About the Spectrophotometer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Touchscreen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Cuvette Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 USB-A port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 PR-1 Pedestal Reconditioning Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 PV-1 Performance Verification Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Instrument Detection Limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Chapter 2 Instrument Set up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
Register Your Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 Update Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 Setting Up User Account Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 User Account Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 Security Administration Policies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Chapter 3 Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
Measure UV-Vis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 Measure UV-Vis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 To make UV-Vis measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 Best practices for UV-Vis measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 UV-Vis Reported Results. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Settings for UV-Vis Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Measure Chemometrics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 Measure Chemometrics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 Create Chemometrics Method. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 Edit Chemometrics Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Chemometrics Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24 Settings for Chemometric Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
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Contents
Measure Custom . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Measure using a Custom Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Delete Custom Method. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Custom Method Reported Results. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Manage Custom Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Chapter 4 Learning Center . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45
Micro-Volume Sampling—How it Works. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Set Up the Instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Measure a Micro-Volume Sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Measure a Sample Using a Cuvette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Prepare Samples and Blanks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Basic Instrument Operations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
NanoDrop One Home Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
NanoDrop One Measurement Screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
NanoDrop One General Operations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
Instrument Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
Data Deletion Settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
PC Control Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
PC Control Home Screen overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
Control options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
History . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
Chapter 5 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .111
Maintenance Schedule. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
Cleaning the Touchscreen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Maintaining the Pedestals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
Cleaning the Pedestals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
Reconditioning the Pedestals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
Decontaminating the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119
Maintaining the Cuvette Sampling System . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
Instrument Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
Intensity Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
Performance Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
Pedestal Image Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130
Chapter 6 Safety and Operating Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .131
Operating Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132
Safety Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133
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1
About the Spectrophotometer
NanoDrop OneC Spectrophotometer
Arm
Pedestal
Cuvette holder
NOTICE Locate the instrument away from air vents and exhaust fans to minimize evaporation
The Thermo Scientific™ NanoDrop™ OneC is a compact, stand-alone
UV-Visible spectrophotometer developed for micro-volume analysis of a wide variety of analytes. The patented sample retention system enables the measurement of highly concentrated samples without the need for dilutions.
The NanoDrop OneC system comes with preloaded software and a touchscreen display. NanoDrop QC PC Control software can be installed on a local PC and used to control the instrument and view data. The instrument can be connected to an optional printer with a USB cable or to a remote printer through an Ethernet connection or wireless network.
NOTICE Before operating a NanoDrop One instrument, please read the safety and operating precautions and then follow their recommendations when using the instrument.
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1 About the Spectrophotometer
Features
Features
TheNanoDrop OneC spectrophotometer features the patented micro-volume sample retention system. The NanoDrop OneC also features a cuvette holder for analyzing dilute samples using standard UV-visible cuvettes.
Touchscreen
TheNanoDrop OneC comes with a built-in, 7-inch high-resolution touchscreen preloaded with easy-to-use instrument control software. The touchscreen can slide left or right to accommodate personal preference, and tilt forward or back for optimal viewing
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1 About the Spectrophotometer
Features
Cuvette Holder
Cuvette holder
Instrument light path
The NanoDrop OneC includes a cuvette holder for measuring dilute samples, colorimetric assays, cell cultures and kinetic studies. The cuvette system has these features:
•extended lower detection limits
•37 °C heater option for temperature-sensitive samples and analyses
•micro-stirring option to ensure sample homogeneity and support kinetic studies
For details, see Measure a Sample using a Cuvette.
USB-A port
Two more USB-A ports are located on instrument back panel
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1 About the Spectrophotometer
Accessories
Accessories
This section lists the accessories included for use with the NanoDrop OneC.
PR-1 Pedestal Reconditioning Kit
Specially formulated conditioning compound that can be applied to the pedestals to restore them to a hydrophobic state (required to achieve adequate surface tension for accurate sample measurements). The kit includes conditioning compound and applicators. For more information, see Reconditioning the Pedestals.
PV-1 Performance Verification Solution
Liquid photometric standard used to check instrument performance. For more information, see Performance Verification.
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1 About the Spectrophotometer
Instrument Detection Limits
Instrument Detection Limits
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1 About the Spectrophotometer
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2
Instrument Set up
Register Your Instrument
Register your instrument to receive e-mail updates on software and accessories for the
NanoDrop OneC instrument. An Internet connection is required for registration.
To register your instrument
1.Do one of the following:
–From any PC that is connected to the Internet, use any web browser to navigate to our website.
On the website, locate NanoDrop One Registration and follow the instructions to register the instrument.
Update Software
Quickly and easily download and install the latest NanoDrop One software and release notes from our website. Follow the steps to update or upgrade the software on your local instrument and/or install or update the NanoDrop QC software on a personal computer (PC). An Internet connection is required to download software.
To install or update NanoDrop QC software on a PC
1.Insert the USB flash drive containing the installer software into an available USB port on your PC
2.Launch Start.exe. The software installer will run.
To install or update NanoDrop QC software on the instrument
1.Copy the .zip file with the new software from your computer to a USB storage device. Do not attempt to unzip the folder.
2.Insert the USB device into any USB port on the NanoDrop OneC instrument.
3.From the instrument Home screen, tap Settings > System > Update Software and choose the latest version of software.
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2 Instrument Set up
Setting Up User Account Control
Setting Up User Account Control
User account control is managed using the Security Administration application. When you launch Security Administration, you will need to enter your Windows log-in information.
User Account Control
Launch the Security Administration application and select NanoDrop QC from the directory on the left to reveal Access Control and System Policies.
Access control
Access control is used to grant or deny individual users or groups access to protected features in the instrument application. Add and remove users and groups to the access list and set access rights using the drop-down for each entity.
System policies
System Policies is used to set options that define the behavior of the client application. See “Security Administration Policies.”
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2 Instrument Set up
Setting Up User Account Control
Security Administration Policies
System policies allow you to assign data and method creation and deletion and editing privileges for users and groups.
Launch the Security Administration application and select NanoDrop QC -> System
Policies
You can add, delete, or edit policy groups and enable or disable the group’s users permission to delete data. When you are finished, select Save. Changes will take effect the next time NanoDrop QC is launched. Changes made to the Security Administration policies are applicable to the local PC only and will not affect other computers on the network.
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2 Instrument Set up
Technical Support
Technical Support
For U.S./Canada Support, please contact:
Thermo Fisher Scientific
3411 Silverside Road
Tatnall Building, Suite 100
Wilmington, DE 19810 U.S.A.
Telephone: 302 479 7707
Toll Free: 1 877 724 7690 (U.S. & Canada only)
Fax: 302 792 7155
E-mail: nanodrop@thermofisher.com
Website: www.thermoscientific.com/nanodrop
For International Support, please contact:
Contact your local distributor. For contact information go to:
http://www.nanodrop.com/Order.aspx
If you are experiencing an issue with your system, refer to the troubleshooting information. If the issue persists, contact us. If you are outside the U.S.A. and Canada, please contact your local distributor.
If your instrument requires maintenance or repair, contact us or your local distributor.
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3
Applications
Use the NanoDrop OneC to perform UV-Vis, Chemometrics, or your own custom measurements.
The UV-Vis application can be set up directly from the touchscreen and allows the instrument to function as a conventional spectrophotometer. Up to 40 wavelengths from 190 nm to 850 nm can be monitored and reported.
The Chemometrics application allows you to use your unique chemometrics method. The method is created using the NanoDrop QC PC Control software that is installed on your PC and then loaded into the instrument though a USB storage device. See the NanoDrop QC PC Control software to learn about Chemometric method features that are supported.
The Custom application provides additional flexibility for the method that you use with the instrument. See the NanoDrop QC PC Control software to learn about custom method features that are supported.
•Measure UV-Vis 12
•Measure Chemometrics 18
•Measure Custom 29
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3 Applications
Measure UV-Vis
Measure UV-Vis
Measures the absorbance of any sample at up to 40 wavelengths across the ultra-violet (UV) and visible regions of the spectrum.
Measure UV-Vis
Reported Results
Settings
Detection Limits
Measure UV-Vis
The UV-Vis application allows the instrument to function as a conventional spectrophotometer. Sample absorbance is displayed on the screen from 190 nm to 850 nm. Up to 40 wavelengths can be designated for absorbance monitoring and inclusion in the report. Automatic pathlength adjustment and a single-point baseline correction can also be used.
To make UV-Vis measurements
NOTICE
•Do not use a squirt or spray bottle on or near the instrument as liquids will flow into the instrument and may cause permanent damage.
•Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will permanently damage the quartz fiber optic cables.
Before you begin...
Before taking pedestal measurements with the NanoDrop One instrument, lift the instrument arm and clean the upper and lower pedestals. At a minimum, wipe the pedestals with a new laboratory wipe. For more information, see Cleaning the Pedestals.
To measure a sample using the UV-Vis application
1.From the Home screen, select UV-Vis.
2.Specify up to 40 wavelengths to monitor (or you can specify them later if desired) and whether automated pathlength adjustment, analysis wavelength, and baseline correction will be used.
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3 Applications
Measure UV-Vis
3.Pipette 1–2 µL blanking solution onto the lower pedestal and lower the arm, or insert the blanking cuvette into the cuvette holder.
Tip: If using a cuvette, make sure to align the cuvette light path with the instrument light path.
4.Tap Blank and wait for the measurement to complete.
Tip: If Auto-Blank is On, the blank measurement starts automatically after you lower the arm. (This option is not available for cuvette measurements.)
5.Lift the arm and clean both pedestals with a new laboratory wipe, or remove the blanking cuvette.
6.Pipette 1-2 µL sample solution onto the pedestal and lower the arm, or insert the sample cuvette into the cuvette holder.
7.Start the sample measurement:
–Pedestal: If Auto-Measure is On, lower arm; if Auto-Measure is off, lower arm and tap Measure.
–Cuvette: Tap Measure.
When the sample measurement is completed, the spectrum and reported values are displayed (see the next section).
8.When you are finished measuring samples, tap End Experiment.
9.Lift the arm and clean both pedestals with a new wipe, or remove the sample cuvette.
Best practices for UV-Vis measurements
•Ensure the sample absorbance is within the instrument’s absorbance detection limits.
•Blank with the same buffer solution used to re-suspend the analyte of interest. The blanking solution should be a similar pH and ionic strength as the analyte solution.
•Run a blanking cycle to assess the absorbance contribution of your buffer solution. If the buffer exhibits strong absorbance at or near an analysis wavelength, you may need to choose a different buffer or application. See Choosing and Measuring a Blank for more information.
•For micro-volume measurements:
–Ensure pedestal surfaces are properly cleaned and conditioned.
–Ensure samples are homogeneous before taking a measurement. Avoid introducing bubbles when mixing and pipetting.
–Follow best practices for micro-volume measurements.
–Use a 1-2 µL sample volume. See Recommended Sample Volumes for more information.
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3 Applications
Measure UV-Vis
•For cuvette measurements (NanoDrop OneC instruments only), use compatible cuvettes and follow best practices for cuvette measurements.
UV-Vis Reported Results
UV-Vis measurement screen
For each measured sample, this application shows the absorbance spectrum and a summary of the results. Here is an example as it appears on the local instrument display:
Menu of options; |
Sample name; |
Absorbance at user- |
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tap to open |
tap to edit |
defined wavelength 1 |
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UV-Vis spectrum
Tap to edit
Absorbance at userdefined wavelength 2 (623 nm)
Tap to edit
Tap to add
Tap row to select sample and update spectrum; tap more rows to overlay up to five spectra. Press and hold sample row to view measurement details.
Pinch and zoom to adjust axes; double-tap to reset
Swipe screen left to |
Tap to end |
Drag tab |
view table with more |
experiment and |
down/up to see |
measurement results |
export data |
more/less |
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3 Applications
Measure UV-Vis
Note Micro-volume absorbance measurements and measurements taken with nonstandard cuvettes are normalized to a 10.0 mm pathlength equivalent.
UV-Vis reported values
The initial screen that appears after each measurement (see previous image) shows a summary of the reported values. To view all reported values, press and hold the sample row. Here is an example:
Application |
Sampling |
Sample name; |
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method |
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correction |
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absorbance |
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Note Scroll up to display absorbance values for any additional user-defined wavelengths.
Thermo Scientific |
NanoDrop OneC with NanoDropQC Software User Guide |
15 |
3 Applications
Measure UV-Vis
Below is an example of the measurement screen with reported values as it appears in the
NanoDrop QC PC software:
Back to Home screen |
Sample name; |
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Measure sample |
End experiment |
(ends experiment) |
select to edit |
Run blank |
solution |
and export data |
Select to toggle Auto-Measure ON/OFF
Tips: Auto-Measure default is ON Auto-Blank default is OFF
Select to add absorbance at userdefined wavelengths
Sample rows
With sample selected, click and drag an area to zoom
Right-click and select Autoscale to fit spectra to window
Tips:
Click sample row to select sample and update spectrum
Shift-click multiple sample rows to overlay up to five spectra
Click a sample and hover locations on spectra to view measurement values
16 |
NanoDrop OneC with NanoDropQC Software User Guide |
Thermo Scientific |
3 Applications
Measure UV-Vis
Settings for UV-Vis Measurements
To show the UV-Vis settings, from the Home screen, select UV-Vis.
Setting |
Available Options |
Description |
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Monitored |
Enter up to 40 |
User-defined wavelengths to be measured and reported at run |
wavelengths |
wavelengths between |
time. Absorbance values for the first three entered wavelengths |
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190 nm and 850 nm |
are displayed in the measurement screen. To see absorbance |
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values for 8 monitored wavelengths, swipe left in the |
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measurement screen to show the Data table. To see all monitored |
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wavelengths, press and hold a sample row to show the Sample |
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Details screen (scroll up to display absorbance values for any |
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additional user-defined wavelengths). |
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Note: If Baseline Correction is selected, all displayed absorbance |
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values are the corrected values. |
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Analytical Wavelength |
Any wavelength between |
This is the wavelength the software will use to determine the |
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190 nm and 850 nm |
pathlength selection. |
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Automated Pathlength |
On or Off |
Optional automated pathlength selection. Allows the software |
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(affects pedestal |
to use the optimal (shorter) pedestal pathlength for high |
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measurements only) |
concentration samples to help prevent detector saturation (see |
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Detection Limits for details). |
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• When selected, the shorter pathlength is used when any |
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wavelength between 220 nm and 850 nm has 10 mm |
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equivalent absorbance value of 12.5 or higher. For |
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wavelengths between 190 nm and 219 nm the change to the |
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shorter pathlength occurs when any wavelength in this range |
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has a 10 mm equivalent absorbance value of 10 or higher. |
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• When deselected, the pedestal pathlength is restricted to |
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10 mm across all wavelengths. |
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Note: In either case, displayed absorbance values have been |
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normalized to a 10 mm pathlength equivalent. |
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Baseline Correction |
On or off |
Optional user-defined baseline correction. Can be used to |
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Enter baseline correction |
correct for any offset caused by light scattering particulates by |
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subtracting measured absorbance at specified baseline correction |
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wavelength in nm or use |
wavelength from absorbance values at all wavelengths in sample |
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default value (750 nm) |
spectrum. As a result, absorbance of sample spectrum is zero at |
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specified baseline correction wavelength. |
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Thermo Scientific |
NanoDrop OneC with NanoDropQC Software User Guide |
17 |
3 Applications
Measure Chemometrics
Measure Chemometrics
Make chemometric measurements.
Measure Chemometrics
Create Chemometric Method
Edit Chemometric Method
Reported Results
Settings
Detection Limits
Measure Chemometrics
The NanoDrop OneC model instrument can be used to make chemometric measurements.
Note The instrument arm can be up during cuvette measurements, which allows you to add reagents to the sample solution if desired.
To make chemometric measurements
NOTICE
•To prevent damage from spills, keep containers of liquids away from the instrument.
•Do not use a squirt or spray bottle on or near the instrument as liquids will flow into the instrument ans may cause permanent damage.
Chemometric methods can only be created on a personal computer running the
NanoDrop QC PC Control software. If you want to run a chemometric method and store the measurement results on the instrument, the method must also reside on the instrument. (This is the only way to run a chemometric method if your instrument is not connected to the computer with an Ethernet cable.)
18 |
NanoDrop OneC with NanoDropQC Software User Guide |
Thermo Scientific |
3 Applications
Measure Chemometrics
Load a chemometrics method
To measure a sample using a chemometrics method, you must first load the method onto the instrument.
To load chemometrics methods onto the instrument:
1.Export the method from the personal computer and copy the method file to the root of a portable USB device such as a memory stick.
Method files have a “.qmethod” filename extension.
2.Connect the USB device to one of the USB ports on the instrument.
3.From the Home screen, select the Chemometric Application icon
4.Use the list box at the top of the screen to indicate the USB port used.
5.Select Load Method.
A message box shows the NanoDrop One methods available on the selected USB device.
6.Select one or more method names in the Load Method box to select the methods to load.
7.Select Load.
Thermo Scientific |
NanoDrop OneC with NanoDropQC Software User Guide |
19 |
3 Applications
Measure Chemometrics
To measure a sample using the chemometrics application
1. From the Home screen, select the Chemometrics icon.
The Chemometrics Method Selection screen is displayed. If one or more chemometric methods exist in the currently selected Data Storage Location, they will be listed in the Select Method box. A description of the selected method appears in the Method Details box.
2.Select a method:
•select an existing method by selecting the method name in the Select Method box.
3.Select Run Method.
4.Follow the on-screen instructions to measure a sample.
20 |
NanoDrop OneC with NanoDropQC Software User Guide |
Thermo Scientific |
3 Applications
Measure Chemometrics
To measure a sample using the chemometrics application from the PC software
1. From the Home screen, select the Chemometric Method icon.
The Chemometrics Method Selection screen is displayed. If one or more chemometric methods exist in the currently selected Data Storage Location, they will be listed in the method selection pane below the search feature. Details of the selected method appears in the method details pane to the right.
2.Select a method:
•select an existing method by selecting the method name in the method selection pane.
3.Select 
.
Follow the on-screen instructions to measure a sample.
Thermo Scientific |
NanoDrop OneC with NanoDropQC Software User Guide |
21 |
3 Applications
Measure Chemometrics
Create Chemometrics Method
Chemometrics methods can be created only on the NanoDrop QC PC Control software. However, once the method is created, it can be saved in the NanoDrop One database on the local instrument, or in the NanoDrop QC database on the PC. To create a new Chemometrics method:
–From the NanoDrop QC software, select the Chemometric Method icon
–From the Chemometric Method Management pane, select 
–Enter both a name and description for your chemometric method
–Select the quant method (.qnt fiile) you want to use
22 |
NanoDrop OneC with NanoDropQC Software User Guide |
Thermo Scientific |
3 Applications
Measure Chemometrics
–Adjust the method settings as desired
–Select Save
Note The method is saved in the currently selected Data Storage Location (local instrument or a connected PC).
–To run the method, select Run Method
Edit Chemometrics Method
Chemometrics methods can be edited only on the NanoDrop QC software. To edit an existing chemometrics method:
–From the NanoDrop QC software, select the Chemometric Method icon
–From the Chemometric Method Management screen, select the method you would like to edit from the list of loaded methods.
–From the drop-down menu 
select Edit.
–Adjust the method settings as desired. You can select the components to be displayed on the LC.
–Select Save
Thermo Scientific |
NanoDrop OneC with NanoDropQC Software User Guide |
23 |
3 Applications
Measure Chemometrics
Chemometrics Reported Results
Chemometrics method measurement screen (shown from Data Viewer)
For each measured sample, this application shows the absorbance spectrum and a summary of the results. Here is an example:
Menu of options; |
Analyte |
tap to open |
concentrations |
UV-visible spectrum
Pinch and zoom to |
Swipe screen left to |
adjust axes; |
view table with more |
double-tap to reset |
measurement results |
Tap row to select sample and update
spectrum; tap more rows to overlay up to five spectra.
Press and hold sample row to view measurement details.
Drag tab down/up to see more/less sample data
Note Micro-volume absorbance measurements and measurements taken with nonstandard cuvettes are normalized to a 10.0 mm pathlength equivalent.
24 |
NanoDrop OneC with NanoDropQC Software User Guide |
Thermo Scientific |
3 Applications
Measure Chemometrics
Chemometrics method reported values
The initial screen that appears after each measurement (see previous image) shows a summary of the reported values. To view all reported values, press and hold the sample row. Here is an example:
Method |
Sampling |
Sample name; |
name |
method |
tap to edit |
Date/time measured
Analyte concentration
Method details
Thermo Scientific |
NanoDrop OneC with NanoDropQC Software User Guide |
25 |
3 Applications
Measure Chemometrics
Data Table
To see the data table, swipe the rate measurement screen (see above) to the left. Each row in the table shows the absorbance values at all user-defined wavelengths at a given stage and time. Scroll down to see measurement information that is out of view. The image below highlights the available features.
Measurement |
Sample name |
Analyte concentration |
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number |
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Press and hold row to view measurement details
Swipe screen right to return |
Tap to end experiment |
to Rate measurement screen |
and export data |
26 |
NanoDrop OneC with NanoDropQC Software User Guide |
Thermo Scientific |
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