Thermo Scientific 4642010, 4642040, 4642020, 4642030, 4642050 Instructions For Use Manual

1

Finnpipette® F2
Single Channel
Variable & Fixed Volume
Multichannel

Instructions for Use

操作指南

User Manual

2

This product complies with the European Union Directive 98/79/EC, and it is marked with
a CE-marking.
When the product is used in applications related to the directive 98/79/EC, read the
additional information at www.thermofisher.com or contact the manufacturer at
info.pipettes@thermofisher.com to ensure correct and safe use.

The CE-mark covers the system containing the CE-marked Finnpipette F2 pipette and the
CE-marked Finntips.

Product specifications are subject to change without prior notice. Finnpipette

®

and Finntip®

are registered trademarks of Thermo Fisher Scientific Oy.

本产品符合欧盟指令98/79/EC的标准，并标有CE标志。
如须依据该指令使用本移液器，使用者还应阅读 www.thermofisher.com 上的
其它有关信息，或者通过 [e-mail] 邮箱联系制造商以
确保正确安全地使用。

CE 标志涵盖了包括印有CE标志的Finnpipette F2以及Finntips的整个体系。
产品规格可能有变，恕不另行通知。

Finnpipette

®

和Finntip® 均为Thermo Fisher Scientific的注册商标。

3

CONTENTS

PRODUCT DESCRIPTION 4
PACKAGE 5
SAFETY 5
PIPETTE OPERATION 6
PIPETTING TECHNIQUES 6
CALIBRATION AND ADJUSTMENT 8
MAINTENANCE 12
TROUBLE SHOOTING 14
SPARE PARTS 27-36

目录

产品简介 15
包装 16
安全

16

移液器的操作 17
移液方法 17
校准与调节 19
维护保养 23
故障检修 25
备件 27-36

4

Product description

The Finnpipette F2 is a continuously adjustable, general purpose micropipette for sampling and
dispensing accurate liquid volumes.
It operates on an air displacement principle (i.e. an air interface) and uses detachable, disposable
tips.
The adjusted delivery volume is displayed digitally on a readout window in the handle.

The thirteen different models of Finnpipette F2 pipettes cover a volume range from 0,2 µl to 10 ml.

Order No. Volume Range

Color code

Finntip

4642010 0,2 µl to 2 µl pink Flex 10, 10, 20, 50
4642020 0,5 µl to 5 µl pink Flex 10, 10, 20, 50
4642030 1 µl to 10 µl pink Flex 10, 10, 20, 50
4642040 1 µl to 10 µl yellow

Flex 200, 250 Univ., 200 Ext, 300, Flex 300

4642050 2 µl to 20 µl

turquoise

50

4642060 2 µl to 20 µl yellow

Flex 200, 250 Univ., 200 Ext, 300, Flex 300

4642120 5 µl to 50 µl

turquoise

50

4642130 5 µl to 50 µl yellow

Flex 200, 250 Univ., 200 Ext, 300, Flex 300

4642070 10 µl to 100 µl yellow

Flex 200, 250 Univ., 200 Ext, 300, Flex 300

4642080 20 µl to 200 µl yellow

Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4642090 100 µl to 1000 µl blue Flex 1000, 1000, 1000 Ext
4642100 0,5 ml to 5 ml green 5 ml
4642110 1 ml to 10 ml red 10 ml, Flex 10 ml Ext

The fifteen different models of Finnpipette F2 Fixed Volume pipettes cover a volume range from
1 µl to 10 ml.

Order No. Volume Range Color code Finntip

4652000 1 µl dark blue Flex 10, 10, 20, 50
4652010 5 µl dark blue Flex 10, 10, 20, 50
4652020 10 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4652130 20 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4652030 25 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4652040 50 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4652050 100 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4652140 200 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4652060 250 µl dark blue Flex 1000, 1000, 1000 Ext
4652070 500 µl dark blue Flex 1000, 1000, 1000 Ext
4652080 1000 µl dark blue Flex 1000, 1000, 1000 Ext
4652090 2000 µl dark blue 5 ml
4652100 3000 µl dark blue 5 ml
4652110 5000 µl dark blue 5 ml
4652120 10000 µl dark blue 10 ml, Flex 10 ml Ext

The ten different models of Finnpipette F2 Multichannel pipettes cover a volume range from
1 µl to 300 µl.

Order No. Channel Volume Range Color code Finntip

4662000 8 1 µl to 10 µl pink Flex 10, 10, 20, 50
4662010 8 5 µl to 50 µl yellow

Flex 200, 250 Univ., 200 Ext, 300, Flex 300

4662020 8 10 µl to 100 µl yellow

Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4662030 8 30 µl to 300 µl orange Flex 300, 300
4662040 12 1 µl to 10 µl pink Flex 10, 10, 20, 50
4662050 12 5 µl to 50 µl yellow

Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4662060 12 10 µl to 100 µl yellow

Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4662070 12 30 µl to 300 µl orange Flex 300, 300
4662080 16 1 µl to 10 µl

purple

20

4662090 16 5 µl to 50 µl

turquoise

50

5

Digital display

The adjusted delivery volume is clearly indicated in the large
digital display on the handle.

Raw materials

The Finnpipette F2 is made of mechanically durable and chemically resistant materials.

Description of tips

Finntips are recommended for use with the Finnpipette F2.
They are made of virgin natural colour polypropylene, generally regarded as the only
contamination free material suitable for tips. Finntips are also autoclavable (121°C).

Package

The Finnpipette F2 is shipped in a specially designed package containing the following items:

1. The Finnpipette

2. Service tool

3. Multichannel service tool

4. Bag of grease 1g (Order No. 3300200)

5. Instructions for Use

6. Calibration certificate / Warranty certificate

Safety

Cautions are marked with this symbol .

Intended use

The intended use of the device is to transfer liquids in the volume range of 1 µl to 1000 µl.
The F2 Finnpipette pipette and Finntips are designed as a component of an analyzing system
for an end user, who is responsible for validating the system to ensure reliable and safe results.

Damage to health

Follow general procedures for hazard prevention and safety instructions; e.g. wear

protective clothing, eye protection and gloves.

For use and waste disposal of hazardous (e.g. radioactive and potentially infectious)

material, follow the safety instructions and general laboratory practice.

The pipette and tips are not intended for in vivo use. Do not use the pipette for pipetting any

liquid to be injected into a human body.

Do not eject the tip towards anybody.

The Finnpipette F2 is to be used by trained personnel with required laboratory skills. The
instructions for use must be read prior to and during the use of the device (pipette and tip). The
F2 pipette can be used between +4°C and +40°C.

Incorrect dispensing results

Performance may vary due to:

a. pipetting method (forward pipetting technique recommended)
b. temperature (air, liquid, vessel, pipette, and tip)
c. pressure
d. humidity
e. operator, e.g. thumb movement, pipetting angle
f. liquid density, viscosity and vapor pressure
g. type of tip

If the pipetting performance is critical to the outcome of a specific application, the result has
to be assured with an alternative test, and if this is not an option, by duplicate testing.
The possibility of an incorrect volume delivery during pipetting cannot be entirely mitigated.

6

To avoid inaccurate dispensing and/or leakage, check that the tip is properly attached to

the pipette.

When rotating the volume adjustment button, do not exceed the volume range of the pipette.

Otherwise the pipette may be damaged or affect pipetting performance.

Pipetting performance cannot be guaranteed if the tip is reused.
Choose only a tip and pipette with matching color coding.

Disposal of the Materials

Follow laboratory and country-specifi c procedures for the disposal of biohazardous or radioactive
waste. Refer to local regulations for the disposal of infectious material.

Warning The tips can be potentially infectious. Dispose of all used disposable tips as
biohazardous waste.

Warning Decontaminate the pipette before disposal.
Waste disposal according to local instructions taking into account legislation in place. For more

information, please contact us at info.pipettes@thermofisher.com or your local Thermo
Fisher Scientific representative.

Regarding the original packaging and packing materials, use the recycling operators known
to you.

Pipette operation

Setting the delivery volume

1. Set the delivery volume using the push button on the top of
the pipette. To increase the delivery volume, turn the push
button counterclockwise. To decrease the delivery volume,
turn it clockwise.

2. Make sure that the desired delivery volume clicks into place.

3. Do not set volumes outside the pipette´s specified volume range.
Using excessive force to turn the push button outside the range
may jam the mechanism and eventually damage the pipette.

Safety Label

You can mark the pipette application, your initials, the
calibration date, etc. on the safety label. Remove the
old label with a sharp needle. Mark the new label with a
pencil and slide the label back in place.

Tip ejection

To help eliminate the risk of contamination, each pipette is
fitted with a tip ejector system. To release the tip, point
the pipette at suitable waste receptacle and press the tip
ejector with your thumb.

Pipetting techniques

Push and release the push button slowly at all times particularly when working with
high viscosity liquids. Never allow the push button to snap back.
Make sure that the tip is firmly attached to the tip cone. Check for foreign
particles in the tip.

Before you begin your actual pipetting work, fill and empty the tip 2-3 times with
the solution that you will be pipetting. Hold the pipette in an upright position while
aspirating liquid. The grippy should rest on your index finger. Make sure that the tips,
pipette and solution are at the same temperature.

+

7

Forward technique

Fill a clean reagent reservoir with the liquid to be dispensed.
1 Depress the push button to the first stop.

2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm and slowly
release the push button. Withdraw the tip from
the liquid touching it against the edge of the
reservoir to remove excess liquid.

3. Deliver the liquid by gently depressing the push
button to the first stop. After a delay of about
one second, continue to depress the push button
all the way to the second stop. This action will empty the tip.

4. Release the push button to the ready position. If necessary, change the tip and continue
pipetting.

Reverse technique

The reverse technique is suitable for dispensing liquids that have a high viscosity or a tendency
to foam easily. The technique is also recommended for dispensing very small volumes. Fill a
clean reagent reservoir with the liquid to be dispensed.

1. Depress the push button all the way to the second stop.

2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm, and slowly
release the push button. This action will fill the
tip. Withdraw the tip from the liquid touching
it against the edge of the reservoir to remove
excess liquid.

3. Deliver the preset volume by gently depressing
the push button to the first stop. Hold the push
button at the first stop. Some liquid will remain
in the tip and this should not be included in
the delivery.

4. The remaining liquid should either be discarded with the tip or pipetted back into the
container.

Repetitive technique

The repetitive technique offers a rapid and simple procedure for repeated delivery of the same
volume. Fill a clean reagent reservoir with the liquid to be dispensed.

1. Depress the push button all the way to the second stop.

2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm, and slowly re
lease the push button. This action will fill the tip.
Withdraw the tipfrom the liquid touching against
the edge of the reservoir to remove excess liquid.

3. Deliver the preset volume by gently depressing
the push button to the first stop. Hold the push
button at the first stop. Some liquid will remain
in the tip and this should not be included in
the delivery.

4. Continue pipetting by repeating steps 3 and 4.

Pipetting of heterogeneous samples

(deproteinization in blood glucose determination, for example)
Use steps 1 and 2 of the forward technique to fill the tip with blood.
Wipe the tip carefully with a dry clean tissue.

1 2 3 4

1 2 3 4

1 2 3 4

8

1. Immerse the tip into the reagent and depress the
push button to the first stop, making sure the tip
is well below the surface.

2. Release the push button slowly to the ready
position.
This will fill the tip. Keep the tip in the solution.

3. Depress the push button to the first stop and
release slowly. Keep repeating this procedure
until the interior wall of the tip is clear.

4. Finally, depress the push button all the way to the
second stop to completely empty the tip.

Calibration and adjustment

All Finnpipettes are factory calibrated and adjusted to give the volumes as specified with
distilled or deionized water using the forward pipetting technique. It should be noted that the
use of other pipetting techniques may affect the calibration results. The pipettes are constructed
to permit re-adjustment for other pipetting techniques or liquids of different temperature and
viscosity.

Device requirements and test conditions

An analytical balance must be used. The scale graduation value of the balance should be chosen
according to the selected test volume of the pipette:

Volume range readable graduation
under 10 µl 0.00 1 mg

10-100 µl 0.01 mg
above 100 µl 0.1 mg

Test liquid: Water, distilled or deionized, “grade 3” water conforming ISO 3696. Tests are done
in a draft-free room at a constant (±0.5°C) temperature of water, pipette and air between 15°C
to 30°C. The relative humidity must be above 50%. Especially with volumes under 50 µl the
air humidity should be as high as possible to reduce the effect of evaporation loss. Special
accessories, such as the evaporation trap, are recommended.

Procedure to check calibration

The pipette is checked with the maximum volume (nominal volume) and with the minimum
volume. A new tip is first pre-wetted 3-5 times and a series of ten pipettings is done with
both volumes. A pipette is always adjusted for delivery (Ex) of the selected volume. Use of
forward pipetting technique is recommended. The maximum permissible errors are designed for
forward method. It is recommended to calibrate all channels of the multichannel pipette, but a
suitable method is also to calibrate only the edge channels. Adjustment check prior calibration is
done using one of the middle channels. It is recommended to use the ISO8655 calibration limits
presented in the following tables or user-defined specifications.

Procedure:

1. Do 10 pipettings with the minimum volume.

2. Do 10 pipettings with the maximum volume.

3. Calculate the inaccuracy (A) and imprecision (cv) of both series.

4. Compare the results to the limits.
If the calculated results are within the selected limits, the adjustment of the pipette is correct.

1 2 3 4 5

9

ISO8655 calibration specifications

Thermo Scientific Finnpipette F2 specifications according to ISO8655 standard.

Finnpipette F2 Variable Volume Single Channel

Range Volume Inaccuracy Imprecision
µl µl % s.d. µl cv%

0,2-2 µl 2 ±0.080 ±4 0.040 2.0

0.2 ±0.080 ±40 0.040 20.0
0,5-5 µl 5 ±0.125 ±2.5 0.075 1.5

0.5 ±0.125 ±25 0.075 15
1-10 µl 10 ±0.120 ±1.2 0.080 0.8

1 ±0.120 ±12 0.080 8.0
2-20 µl 20 ±0.20 ±1.0 0.10 0.5

2 ±0.20 ±10.0 0.10 5.0
5-50 µl 50 ±0.50 ±1.0 0.20 0.4

5 ±0.50 ±10 0.20 4.0
10-100 µl 100 ±0.80 ±0.8 0.30 0.3

10 ±0.80 ±8.0 0.30 3.0
20-200 µl 200 ±1.60 ±0.8 0.60 0.3

20 ±1.60 ±8.0 0.60 3.0
100-1000 µl 1000 ±8.0 ±0.8 3.0 0.3

100 ±8.0 ±8.0 3.0 3.0
0,5-5 ml 5000 ±40.0 ±0.8 15.0 0.3

500 ±40.0 ±8.0 15.0 3.0
1-10 ml 10000 ±60.0 ±0.6 30.0 0.3

1000 ±60.0 ±6.0 30.0 3.0

Finnpipette F2 Fixed Volume Single Channel

Fixed Volume Inaccuracy Imprecision
µl µl % s.d.µl cv%

1 ±0.050 ±5.00 0.050 5.00
5 ±0.125 ±2.50 0.075 1.50
10 ±0.120 ±1.20 0.080 0.80
20 ±0.20 ±1.00 0.10 0.50
25 ±0.50 ±2.00 0.20 0.80
50 ±0.50 ±1.00 0.20 0.40
100 ±0.80 ±0.80 0.30 0.30
200 ±1.60 ±0.80 0.60 0.30
250 ±4.00 ±1.60 1.50 0.60
500 ±4.00 ±0.80 1.50 0.30
1000 ±8.00 ±0.80 3.00 0.30
2000 ±16.0 ±0.80 6.00 0.30
3000 ±40.0 ±1.33 15.00 0.50
5000 ±40.0 ±0.80 15.00 0.30
10000 ±60.0 ±0.60 30.00 0.30

Finnpipette F2 Variable Volume Multichannel

Range Channel Volume Inaccuracy Imprecision
µl µl % s.d.µl cv%

1-10 µl 8, 12, 16 10 ±0.24 ±2.4 0.16 1.6
1 ±0.24 ±24 0.16 16

5-50 µl 8, 12, 16 50 ±1.0 ±2.0 0.4 0.8
5 ±1.0 ±20 0.4 8.0

10-100 µl 8, 12 100 ±0.80 ±0.8 0.30 0.3
10 ±0.80 ±8.0 0.30 3.0

30-300 µl 8, 12 300 ±8.0 ±2.7 3.0 1.0
30 ±8.0 ±26.7 3.0 10.0

10

Manufacturer specification limits

Finnpipette F2 Variable Volume Single Channel

Range Volume Inaccuracy Imprecision Factory
µl µl % s.d. µl cv% calibration tip

0.2-2 µl 2 ±0,050 ±2,50 0,040 2,00 Flex 10
0,2 ±0,024 ±12,00 0,020 10,00

0.5-5 µl 5 ±0,075 ±1,50 0,050 1,00 Flex 10
0,5 ±0,030 ±6,00 0,025 5,00
1-10 µl, micro 10 ±0,100 ±1,00 0,050 0,50 Flex 10
1 ±0,025 ±2,50 0,020 2,00
1-10 µl 10 ±0,100 ±1,00 0,080 0,80 Flex 200
1 ±0,035 ±3,50 0,030 3,00
2-20 µl, micro 20 ±0,20 ±1,00 0,08 0,40 50
2 ±0,06 ±3,00 0,05 2,50
2-20 µl 20 ±0,20 ±1,00 0,08 0,40 Flex 200
2 ±0,06 ±3,00 0,05 2,50
5-50 µl, micro 50 ±0,30 ±0,60 0,15 0,30 50
5 ±0,15 ±3,00 0,125 2,50
5-50 µl 50 ±0,30 ±0,60 0,15 0,30 Flex 200
5 ±0,15 ±3,00 0,125 2,50
10-100 µl 100 ±0,80 ±0,80 0,20 0,20 Flex 200
10 ±0,30 ±3,00 0,10 1,00
20-200 µl 200 ±1,2 ±0,60 0,4 0,20 Flex 200
20 ±0,36 ±1,80 0,14 0,70
100-1000 µl 1000 ±6,0 ±0,60 2,0 0,20 Flex 1000
100 ±1,0 ±1,00 0,6 0,60
0,5-5 ml 5000 ±25,0 ±0,50 10,0 0,20 5 ml
500 ±10,0 ±2,00 4,0 0,80
1-10 ml 10000 ±50,0 ±0,50 20,0 0,20 10 ml
1000 ±20,0 ±2,00 8,0 0,80

Finnpipette F2 Fixed Volume Single Channel

Fixed Volume Inaccuracy Imprecision Factory
µl µl % s.d. µl cv% calibration tip

1 ±0,040 ±4,00 0,040 4,00 Flex 10
5 ±0,070 ±1,40 0,070 1,40 Flex 10
10 ±0,090 ±0,90 0,080 0,80 Flex 200
20 ±0,14 ±0,70 0,10 0,50 Flex 200
25 ±0,15 ±0,60 0,125 0,50 Flex 200
50 ±0,30 ±0,60 0,20 0,40 Flex 200
100 ±0,40 ±0,40 0,30 0,30 Flex 200
200 ±0,80 ±0,40 0,60 0,30 Flex 200
250 ±1,0 ±0,40 0,75 0,30 Flex 1000
500 ±1,5 ±0,30 1,5 0,30 Flex 1000
1000 ±3,0 ±0,30 3,0 0,30 Flex 1000
2000 ±6,0 ±0,30 4,0 0,20 5 ml
3000 ±9,0 ±0,30 6,0 0,20 5 ml
4000 ±12,0 ±0,30 8,0 0,20 5 ml
5000 ±15,0 ±0,30 10,0 0,20 5 ml
10000 ±30,0 ±0,30 20,0 0,20 10 ml

11

+

Finnpipette F2 Variable Volume Multichannel

Range Channels Volume Inaccuracy Imprecision Factory
µl µl % s.d. µl cv% calibration tip

1.0-10 µl 8, 12, 16 10 ±0,240 ±2,40 0,160 1,60 Flex 10
1 ±0,120 ±12,00 0,080 8,00
5-50 µl 8, 12, 16 50 ±0,75 ±1,50 0,35 0,70 Flex 200
5 ±0,25 ±5,00 0,10 2,00
10-100 µl 8, 12 100 ±1,30 ±1,30 0,50 0,50 Flex 200
10 ±0,50 ±5,00 0,20 2,00
30-300 µl 8, 12 300 ±3,0 ±1,00 0,9 0,30 Flex 300
30 ±1,5 ±5,00 0,6 2,00

Adjustment

Adjustment is done only for one volume. The recommended adjustment volume is the minimum
volume or 10% of the maximum volume. Multichannel pipettes adjustment check prior
calibration is done using one of the middle channels.

1. Place the service tool into the openings of the
calibration nut at the top of the handle.

2. Turn the service tool clockwise to increase, or
counterclockwise to decrease the volume.

3. After adjustment check the calibration according
to the instructions above.

Formulas for calculating results

Conversion of mass to volume
V = (w + e) x Z V = volume (µl)

w = weight (mg)
e = evaporation loss (mg)
Z = conversion factor for µl/mg conversion

Evaporation loss can be significant with low volumes. To determine mass loss, dispense water
to the weighing vessel, note the reading and start a stopwatch. See how much the reading
decreases during 30 seconds (e.g. 6 mg = 0.2 mg/s).
Compare this to the pipetting time from taring to reading. Typically pipetting time might be 10
seconds and the mass loss is 2 mg (10 s x 0.2 mg/s) in this example. If an evaporation trap or lid
on the vessel is used the correction of evaporation is usually unnecessary.
The factor Z is for converting the weight of the water to volume at test temperature and pressure.
A typical value is 1.0032 µl/mg at 22°C and 95 kPa. See the conversion table on page 26.

Inaccuracy (systematic error)

Inaccuracy is the difference between the dispensed volume and the selected volume of a pipette.

A = V - V0 A = inaccuracy

V = mean volume
V0 = nominal volume

Inaccuracy can be expressed as a relative value: A% = 100% x A / V

0

Imprecision (random error)

Imprecision refers to the repeatability of the pipettings. It is expressed as standard deviation (s)
or coefficient of variation (cv)

s = standards deviation
v = mean volume
n = number of measurements

Standard deviation can be expressed as a relative value (CV)

CV = 100% x S / V

12

Maintenance

When the Finnpipette F2 is not in use, make sure it is stored in an upright position. We
recommend a Finnpipette stand for this purpose.

The part # refer to exploded views beginning at page 27.

Short-term service

The pipette should be checked at the beginning of each day for dust and dirt on the outside
surfaces of the pipette.
Particular attention should be paid to the tip cone. No other solvents except 70 % ethanol should
be used to clean the pipette.

Long-term service, single channel pipettes

Annual pipette usage varies significantly among laboratories, and the
types of substances being aspirated and dispensed also vary tremendously
among laboratories. In order to maintain proper care of the pipette, it is the
laboratory’s responsibility to develop a proper maintenance program that
meets its specific needs and requirements.

The servicing procedure starts with the disassembly of the pipette.

1-1000 µl pipettes

1. Press the tip ejector.

2. Rotate the tip ejector 11 counterclockwise and pull it out.

3. Turn out the tip cone counterclockwise with the service tool.

4. Pull out the piston and other parts. Push out with piston the rest of the
piston assembly. Then turn the tip cone upside down and tap all parts
from tip cone. Remember keep all parts in order on table for reassembly.

5. Clean the piston, the piston spring and the O-rings with a dry
napless cloth.

6. Check the tip cone for foreign particles.

7. Grease the cleaned parts with the lubricant that comes with
the pipette.

8. Reassemble the pipette components.

0.2-2µl, 0.5-5µl & 1-10 µl: First, slide spring 22,
o-ring support 23 and o-ring 24 on the tube 21.
With the 0.2-2µl model insert the tube 27 into
the tube 21. Then slide the spring 13, spring
support 16 and tubes 17 and 18, bigger o-ring 19
and smaller o-ring 20 back on the piston.
Compress the spring with fingers by pressing
piston and spring support 16 against each other
and slide the tube 21 with rest of the parts on
the

piston. Hold the spring compressed and

carefully slide the entire assembly

into the tip

cone and release the spring.
2-20 µl & 5-50 µl: Slide the spring 13, spring

support 16 and tubes 17 and 18, bigger o-ring
19 and smaller o-ring 20 back on the piston.
Compress the spring with fingers by pressing
piston and spring support 16 against each
other and slide the bigger o-ring 19, smaller
o-ring 20, spring support 21 and the spring

22

(smaller diameter against spring support 21) on the piston.

Hold the spring compressed and

carefully slide the entire assembly into the tip cone and release the spring.
10-100 µl & 20-200 µl: Slide the spring 13, spring support 16 and o-ring 17 back on the

piston. Slide the entire assembly into the tip cone.
100-1000µl: Put the o-ring 17 and support ring

16 to the tip cone. Slide the spring 13 on

the piston and slide the entire assembly into thetip cone.

1

3

2

1-200µl

200-1000µl