tecan Columbus Washer Technical Manual

Technical Manual for

Columbus Washer

Document Part No.: 30000294

2006-02

Document Revision No.: 1.7

Firmware Level: 4.xx

TECAN Affiliates and Service Centers

Austria

Tecan Austria GmbH

Untersbergstrasse 1a
A-5082 Grödig/Salzburg

Austria

T [phone]
F [phone]
[e-mail]

Belgium

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Netherlands

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Theale
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China

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Switzerland

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USA

Tecan Systems Inc.

2450 Zanker Road
San Jose, CA 95131

USA

T [phone]
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Toll Free US/Canada:
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[e-mail]

2 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

WARNING

CAREFULLY READ AND FOLLOW THE INSTRUCTIONS

PROVIDED IN THIS MANUAL BEFORE OPERATING THE

INSTRUMENT.

Notice

Every effort has been made to avoid errors in text and diagrams, however, Tecan
Austria GmbH assumes no responsibility for any errors whi ch may appe ar in this
publication.

It is the policy of Tecan Austria GmbH to improve products as new techniques
and components become available. Tecan Austria GmbH therefor e reserves
the right to change specifications at any time.

We would appreciate any comments on this publication.
Tecan Austria GmbH
Untersbergstraße 1A
A-5082 Grödig/Salzburg
AUSTRIA / EUROPE
Telephone: [phone]
FAX: [phone]
E-mail: [e-mail]
www.tecan.com

Copyright Information

The contents of this manual are the property of Tecan Austria GmbH and are not
to be copied, reproduced or transferred to another person or persons without our
prior written permission.

Copyright © Tecan Austria GmbH
All rights reserved.
Printed in Austria.

Declaration for EU Certificate

See the back of this manual.

About this Manual

This technical manual describes the servicing of the Columbus Washer, designed
to wash microplates. It is intended only for service personnel.

This manual instructs how to:

• Install the instrument

• Operate the instrument

• Programming the wash procedure

• Definition on the wash parameters

• Cleaning and maintenance procedures

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3

Warnings, Cautions and Notes

There are three types of informational notices used in this manual. These notices
highlight important information or warn the user of a potentially dangerous
situation. The following notices are:

Note

Gives helpful information.

Caution

Indicates a possibility of instrument damage or data loss if instructions are

INDICATES THE POSSIBILITY OF SEVERE PERSONAL INJURY, LOSS OF

LIFE OR EQUIPMENT DAMAGE IF THE INSTRUCTIONS ARE NOT

not followed.

WARNING

FOLLOWED.

4 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

Table of Contents

1. General

1.1 Overview......................................................................................... 1-1

1.2 Instrument Description .................................................................1-2

1.2.1 Rear Panel Connections.................................................................. 1-2

1.2.2 Connection Diagram for Columbus (1 Liquid Channel)*.................. 1-4

1.2.3 Connection Diagram for Columbus (4 Liquid Channels)*................ 1-5

1.2.4 Keyboard and Display Unit..............................................................1-6

1.3 Interconnection Diagram .............................................................. 1-7

2. Installation Procedure

2.1 Unpacking and Inspection............................................................ 2-1

2.2 Unpacking Procedure ................................................................... 2-2

2.3 Power Requirements..................................................................... 2-2

2.4 Installation Procedure................................................................... 2-3

2.4.1 Installing the Instrument .................................................................. 2-3

2.4.2 Connecting the Waste Tubing......................................................... 2-3

2.4.3 Connecting the Solution Tubes ....................................................... 2-4

2.4.4 Fitting and Connecting the Liquid Sensors (Optional).....................2-5

2.4.5 Connecting the Barcode Reader (Optional)..................................... 2-6

2.4.6 Connecting the Printer (Optional)....................................................2-6

2.4.7 Power Connections ......................................................................... 2-6

3. Firmwar e and S oftwar e De s cription

3.1 Instrument Software Features...................................................... 3-1

3.2 Onboard Software Menus............................................................. 3-2

3.2.1 Run..................................................................................................3-2

3.2.2 Prime............................................................................................... 3-2

3.2.3 Rinse ............................................................................................... 3-3

3.2.4 Program........................................................................................... 3-6

3.2.5 Clear................................................................................................3-6

3.2.6 Show................................................................................................ 3-6

3.2.7 Adjust............................................................................................... 3-7

3.2.8 Service Menu................................................................................. 3-24

3.3 External Software ........................................................................ 3-61

3.3.1 Columbus Computer Software ...................................................... 3-61

3.3.2 Firmware Update...........................................................................3-61

3.4 Serial Interface............................................................................. 3-62

3.4.1 Hardware Specifications................................................................ 3-62

3.4.2 Contents........................................................................................ 3-65

3.4.3 A. Protocol.....................................................................................3-67

3.4.4 Sequence Number......................................................................... 3-68

3.4.5 B. Commands - Overview.............................................................. 3-69

3.4.6 Commands - Description............................................................... 3-71

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 5

3.4.7 D. Examples for Remote Control - Command Sequences.............3-88

3.5 Description of Error Messages...................................................3-95

4. Removing and Re placing the Instruments Cover

4.1 Removal of Instruments Top Cover..............................................4-1

4.2 Replacing the Instruments Top Cover .........................................4-3

5. Liquid System

5.1 Introduction ....................................................................................5-1

5.1.1 Liquid System Diagram....................................................................5-2

5.1.2 Dispensing System Description........................................................5-3

5.2 Replacement Procedure................................................................5-4

5.2.1 Replacing the Dispensing Pump......................................................5-4

5.2.2 Replacing the Solution Solenoid Valves (Instruments with Pinch

Valves) .............................................................................................

5.2.3 Replacing the Solution Solenoid Valves...........................................5-7

5.2.4 Replacing the Dispense Solenoid Valve ..........................................5-8

5.2.5 Replacing the Aspirating Pump........................................................5-9

5.2.6 Replacing the Aerosol Pump..........................................................5-10

5-6

6. Mechanical System

6.1 Manifold Arm Movement System..................................................6-1

6.1.1 Description of Manifold Arm Movement Parts..................................6-2

6.1.2 Adjusting the Manifold......................................................................6-2

6.2 Plate Transport System.................................................................6-3

6.2.1 Plate Support ...................................................................................6-4

6.2.2 Leveling the Plate Support...............................................................6-5

6.2.3 Microplate Positioning System.........................................................6-7

6.3 Replacement Procedure................................................................6-8

6.3.1 Replacing the Manifold Arm Error Sensor........................................6-8

6.3.2 Replacing the Motor.........................................................................6-9

6.3.3 Replacing the Top Position Optical Switch.....................................6-10

6.3.4 Replacing the Plate Support Home Position Optical Switch ..........6-11

7. Electronic System

7.1 Introduction ....................................................................................7-1

7.2 Interconnection Diagram...............................................................7-2

7.3 Main Board......................................................................................7-3

7.3.1 Replacing the Main Board................................................................7-4

7.3.2 Replacing the Battery.......................................................................7-5

7.4 Power Supply Board......................................................................7-6

7.4.1 Replacing the Power Supply Board..................................................7-7

7.5 Keyboard and Display Unit............................................................7-8

7.5.1 Replacing the Keyboard or Display Unit...........................................7-9

7.6 Manifold Detect Board.................................................................7-11

7.6.1 Replacing the Manifold Detect Board.............................................7-11

6 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

7.7 Fill Verification Board ................................................................. 7-13

7.7.1 Replacing the Fill Verification Board.............................................. 7-13

7.8 Optical Switches.......................................................................... 7-15

8. Serial Interface

8.1 Hardware Specifications............................................................... 8-1

8.1.1 Pin Designation ............................................................................... 8-1

8.1.2 RS-232-C Interface Lines................................................................8-2

8.1.3 Synchronization and Data Format...................................................8-3

8.1.4 Daisy Chaining Instruments............................................................. 8-3

9. Description of Options

9.1 Instrument Options ....................................................................... 9-1

9.2 Liquid Detection Option................................................................ 9-3

9.3 Barcode Reader Option ................................................................ 9-4

9.4 Printer Option ................................................................................ 9-5

9.5 Manifold Detection Option............................................................ 9-6

9.6 Fill Verification Option .................................................................. 9-8

9.7 Aerosol Pump Option.................................................................... 9-8

9.8 Activating the Options .................................................................. 9-8

10. Maintenance

10.1 Cleaning Maintenance................................................................. 10-1

10.1.1 Cleaning the Manifold.................................................................... 10-1

10.1.2 Exchanging the Manifold ............................................................... 10-3

10.1.3 Exchanging the Plate Support.......................................................10-5

10.1.4 Instrument Disinfection..................................................................10-6

10.2 Preventive Maintenance Plan..................................................... 10-8

10.2.1 Daily............................................................................................... 10-8

10.2.2 Weekly........................................................................................... 10-8

10.2.3 Every Six Months........................................................................... 10-8

10.2.4 Yearly (Service Technician Required)...........................................10-8

10.3 Service Tools ............................................................................... 10-9

10.3.1 Adjustment Plate (Reference Plate).............................................. 10-9

10.4 Electrostatic Discharge Information........................................ 10-10

10.4.1 Generating Static......................................................................... 10-10

10.4.2 Preventing Electrostatic Damage to Equipment.......................... 10-11

10.4.3 Personal Grounding Methods...................................................... 10-11

11. Trouble Shooting

11.1 Introduction.................................................................................. 11-1

12. Instrument Specifications

12.1 Introduction.................................................................................. 12-1

Index

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 7

1. General

1. General

1.1 Overview

This is a technical manual for servicing the Columbus Washer.
The instrument is a microprocessor controlled washer, designed to wash

microplates.
The instrument can be fitted with either an 8, 12 or 16 way manifold or a 384 Well

Manifold.
The manifolds are fitted with one aspirating and one dispensing needle per well.
The instrument can store up to nine software user-defined plate types including

dispensing and aspirating positions as well as the shape of the plate.
The dispensing speed can be selected from five set speeds and dropwise.
These features allow the instrument to be used for all washing applications

including Cell Washing applications.

The instrument should only be opened by authorized personnel. Before the

instrument is opened the mains power cable must be disconnected from

Ensure that the instrument has been disinfected before it is serviced.

the mains power socket.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 1-1

1. General

r

r

(

)

A

(

)

1.2 Instrument Description

The diagram below shows the main components of the instrument:

Manifold Arm

Manifold

POWER

Power ON LED

Keyboa r d an d

Display Unit

Waste Sink

Plate Support

Guide Bar

1.2.1 Rear Panel Connections

The instrument has the following connections on the back panel.

Mains Power

Socket

Fuse

Compartment

ON/OFF

Switch

Hand held

Barcode

Reader

Bottle Holder with

Liquid Sensor for

each bottle

Printer Connecto

or

Serial RS 232 fo

Remote Control

Inlets for channels 1 - 4

erosol

Tubing

option

Waste
Tubing

red

1-2 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

1. General

C

A

(Instrument with Pinch Valves)

Mains Power

Socket

Fuse

ompartment

ON/OFF

Switch

Hand held

Barcode

Reader

Bottle Holder with

Liquid Sensor for

each bottle

Prime
valve

Printer Connector

or

Serial RS 232 for

Remote Control

Channel 1

Inlet

Channel 2

Inlet

Channel 3

Inlet

erosol

Tubing

(yellow)

Channel 4

All connected devices must be proved and listed with regulations
EN 60950, UL 1950 or CSA C22.2 No. 950 for Data Processing Devices

Inlet

Waste

Tubing

(red)

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 1-3

1. General

)

p

g

q

g

1.2.2 Connection Diagram for Columbus (1 Liquid Channel)*

Hand held
Barcode

Reader (option)

Prime
valve

Printer (option

or

Com

uter for

Remote Control

Marked

reen

grey

Wash

uid

Li

Bottle Holder with liquid

sensor for each bottle

reen

Rinse
solution

red

Inlet
filter

* Some of the instruments may include the prime valve.

Waste
Bottle

Marked

red

1-4 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

1. General

1.2.3 Connection Diagram for Columbus (4 Liquid Channels)*

Hand held Barcode

Reader (option)

Printer (option)

Computer for

Remote Control

Marked green

Inlet
filter

Wash

Liquid 1

Bottle Holders with liquid

sensor for each bottle

or

Marked grey

Rinse

solution

grey red

green

Marked
red

Waste
Bottle

Marked
blue

None Return

Valve

yellow

Marked
yellow

Foam
Bottle

purple

blue

Aerosol

Filter

Seperator

Water

Wash

Liquid 3

Marked
purple

Inlet
filter

Marked

blue

Wash

Liquid 2

* Some of the instruments may include the prime valve.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 1-5

1. General

r

1.2.4 Keyboard and Display Unit

The Washer is operated via a keyboard and a control panel consisting of a two
row, sixteen digit Liquid Crystal Display (LCD) unit.

The top row of the display unit is used for messages and the bottom row for the
key functions or options.

A Light Emitting Diode (LED) in the center of the letter "O" of the word "POWER"
indicates when the Washer is switched ON.

Key 1 Key 2 Key 3

Key 4

POWER

Powe
ON LED

1-6 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

1. General

A

A

1.3 Interconnection Diagram

The diagram below shows how the various parts of the instrument are
interconnected.

Dispensing Pump

spirating Pump

erosol Pump

Prime Valve

Plate Home Position
Optical Switch

Plate Transport

Motor

Manifold Arm

Orbital Shaking

Motor

*

Motor

Home Position
Optical Switch

Manifold Typeand
Plate MismatchBoard

Fill Verify and
Plate Mismatch
Board

Solenoid 4

Solenoid 3

Solenoid 2

Solenoid 1
Dispense

Solenoid

Liquid
Detection
System

Keyboard /
Display Unit

Printer / Serial
Connector

EEPROM

CPU

Barcode Reader

Power Supply Board

Fuses

Mains Input

ON/OFF Switch

* The Orbital Shaking option is not used.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 1-7

2. Installation Procedure

(op

)

A

p

2. Installation Procedure

2.1 Unpacking and Inspection

The instrument is shipped in one carton, which contains:

• Instrument

• Accessory package which contains:

Spare manifold cover with two screws and Allen key
Operating manual
Mains power cable
Cleaning needles
2 spare fuses
Solution tube
3 spare 0 – ring seals for manifold

• Bottle set containing:

3 x 2.5 l bottles for the standard instrument

Liquid Sensor System

tional

Bottles

ccessory package

and o

erating manual

Instrument

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2-1

2. Installation Procedure

2.2 Unpacking Procedure

1. Visually inspect the container for damage before it is opened.

Report any damage immediately.

2. To place the instrument, select a location that is flat, level, vibration free,
away from direct sunlight, and free from dust, solvents and acid vapors.
Allow 10 cm distance between the back of the instrument and the wall or any
other equipment.

3. Place the carton in an upright position and open it.

4. Lift the instrument out of the carton and place it in the selected location.

5. Visually inspect the instrument for loose, bent or broken parts.

Report any damage immediately.

6. Compare the serial number on the rear panel of the instrument with the
serial number on the packing slip.

Report any discrepancy immediately.

7. Check the instrument accessories against the packing list.

8. Save packing materials for future use.

2.3 Power Requirements

The instrument is designed to operate at either 100 - 120 volt or 220 - 240 volt.

No voltage setting is required as the washer automatically senses the supplied
voltage.

The washer requires 2 x T 1.6 A / 250 V (slow blow) fuses.

WARNING

TO PREVENT THE RISK OF FIRE, THE MAINS FUSES SHOULD ONLY BE

REPLACED WITH THE SAME TYPE AND RATING OF FUSE.

2-2 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

2. Installation Procedure

A

2.4 Installation Procedure

Before the instrument is installed and switched on, it should be left to stand

for at least three hours, so there is no possibility of condensation causing a

2.4.1 Installing the Instrument

• Unpack the instrument and check the contents.

See 2.2 Unpacking Procedure.

• Connect the solution tubes to the inlet connectors.

See 2.4.3 Connecting the Solution Tubes.

• Setup possible instrument options. See page 2 – 5 and 2 – 6.

• Connect instrument to the power supply. See 2.4.7 Power Connections.

• Define the plate parameters in the Adjust menu. See chapter 3.

short circuit.

• Program the wash procedure. See chapter 3.

2.4.2 Connecting the Waste Tubing

Turn the instrument onto the right hand side.
Slide the waste tube through the hole in the back panel.

erosol Tube Hole

(optional)

Connect the waste tube onto the top connector of the aspirating pump.

Waste Tube Hole

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2-3

2. Installation Procedure

A

A

A

A

A

Standard Instrument

Waste Bottle

Dispensing

Pump

Waste Tube

spirating Tube to

Solution Tubes to Manifold

Aspirating Pump

Turn the instrument back to the normal position.
Place the other end of the waste tubing into the waste bottle.
Connect the aerosol tube onto the aerosol pump if this option is fitted. This is not

necessary for the standard instrument.
Instrument with Aerosol Protection Option

Waste Bottle

Dispensing Pump

erosol Bottle

Aspirating Tube to Manifold

Solution Tubes to Manifold

spirating Pump

erosol

Pump

Turn the instrument back to the normal position.
Place the other end of the aerosol tubing into the aerosol bottle.

2.4.3 Connecting the Solution Tubes

The solution tubes are connected onto the inlet connectors on the back panel of
the instrument. The instrument is supplied with four meters of tubing that can be
cut to the required length (maximum 1 meter).

Inlets for channe ls 1 - 4

erosol Tube*

(optional)

Waste Tube

red

2-4 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

2. Installation Procedure

2.4.4 Fitting and Connecting the Liquid Sensors (Optional)

Connect the detector unit cable into the appropriate socket in the back panel of
the instrument. The liquid sensor system can be extended to control up to four
different liquid bottles and one waste bottle.

rinse

grey

liquid 1

green

Liquid Sensor

Fix the tube support in the bottle holder as shown below.

Tube support

waste

red

Insert the tubes and connect the bottle holder to the instrument.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2-5

2. Installation Procedure

2.4.5 Connecting the Barcode Reader (Optional)

Before the barcode reader can be connected to the instrument, the supplied
barcode chips must be added to the main board. For more details contact your
service technician.

Hand Held Barcode

Reader

Liquid Sensor

2.4.6 Connecting the Printer (Optional)

Before the printer can be connected to the instrument, the supplied chip must be
added to the main board. For more details contact your service technician.

Connect the printer to the instrument using the supplied cable.
Connect the power supply cable to the printer.

ON /OFF

Switch

OFF 1 2

POWER

Connection Cable

BATT. LOW

POWER

ON

PAPER

FEED

Power Cable

2.4.7 Power Connections

Before the instrument is installed and switched on, it should be left to

stand for at least three hours, so there is no possibility of condensation

Ensure that the on / off switch in the back panel of the instrument is in the off
position.

Insert the mains power cable into the mains power socket on the rear of the
instrument.

2-6 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

causing a short circuit.

3. Firmware and Software Description

3. Firmware and Software
Description

3.1 Instrument Software Features

The instrument has the following software features:

• 30 user definable washing programs, permanently stored under the
numbers 1 to 30.

• Up to 60 processing steps per program, each processing step can be
identical or different from the previous step.

• 3 washing modes, normal washing, bottom washing and overflow washing.
Set by the Plate Type parameters.

• Soak time. selectable from 1 to 999 seconds.

• Software selectable dispensing speeds

• Software selectable aspirating speeds

• Software selectable bottom position, for aspiration contact to the bottom

of the wells.

• 4 rinse modes, the different rinse modes can be used to select how the
instrument is rinsed before it is left to stand or switched OFF.

• Automatic microplate centering, the microplate is automatically centered
before the start of the washing program.

• Programmable strip selection, the program can be defined so that the
strips to be washed can be selected before starting the washing procedure.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-1

3. Firmware and Software Description

3.2 Onboard Software Menus

The instrument can be used in the following onboard software menus.

3.2.1 Run

Selection of the run procedure to perform the selected program.

3.2.2 Prime

Before the instrument can be used, all dispensing channels must be primed
with the required solution.

Ensure that the bottle of priming solution is always full at the beginning of
the priming procedure and check that the inlet filters in the liquid tubes are
clear.

Please ensure that the dispensing and aspiration pumps are not run for

longer than a few minutes without liquid or they will be damaged.

The priming procedure is performed using the following procedure:

Run XX yyyyyyyy

- + other yes

Prime Ch1

- + other yes

Prime Solution ?
exit yes

Waste Bottle OK?
exit yes

Prime ChX Y sec
Stop

Priming Procedure

performed

Run XX yyyyyyyy

- + other yes

Select required channel to be primed

Required solution in bottle and inlet
tube inserted into solution

Waste bottle empty

Priming procedure being performed

Instrument ready to wash microplate

Caution

Do not use the instrument to aspirate or dispense any acidic solutions as

3-2 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

this could damage the instrument.

3. Firmware and Software Description

y

y

y

y

g

p

yyyyyyy

3.2.3 Rinse

There are four Rinse modes to flush the liquid system and prevent needle
blockages. They should be carried out when the instrument is left to stand or is
switched off at the end of working.

The Rinse modes are:

Rinse Soak
Rinse Day
Rinse Night
Rinse Auto

Rinse: Soak

This procedure is used if the instrument is to be left to stand for a short time.
(1/2 hr. or less).

With this procedure you can use either distilled water or wash solution.

Rinse: Soak

- + other

Soak: CH1

- + exit

Rinse Solution ?
exit

Waste Bottle OK?
exit

Soakin
Sto

Run XX:

- + other yes

es

es

es

es

Select and confirm Soak Rinse procedure

Select required channel (1 - 4)

Confirm that the rinsing solution
and the tube are in the bottle

Confirm waste bottle is empty

The waste sink is filled with solution
and the manifold is lowered into it

Manifold left in solution until Stop key pressed

Standby mode
Washing procedure can be performed

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-3

3. Firmware and Software Description

Rinse: Day

This procedure is used to rinse the wash solution system if the instrument is to be
left standing for a longer time. Alternatively it can be used to rinse the wash
solution system after the instrument has been left for a long time.

With this procedure you can use either distilled water or wash solution.
If the instrument is left to stand for 1 hr to 1/2 day, wash solution can be used, for

longer times use distilled water.

Rinse: Day

- + other yes

Day: CH1

- + exit yes

Rinse Solution ?
exit yes

Waste Bottle OK?
exit yes

Rinse ChX YY sec
Stop

Select and confirm Day Rinse procedure

Select required channel (1 - 4)

Confirm rinsing solution and tube in bottle

Confirm waste bottle is empty

Wash system rinsed for set rinse time

The waste sink is filled with solution and
the manifold is lowered into it

Soaking
Stop

Run XX:yyyyyyy

- + other yes

Manifold left in solution until

key pressed

Stop

Standby mode
Washing Procedure can be performed

3-4 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

Rinse: Night

y

g

y

y

y

p

p

g

p

yyyyyyy

p

3. Firmware and Software Description

This procedure is used to thoroughly rinse the wash solution system and leave
the manifold soaking in the waste sink full of solution at the end of working. This
procedure requires distilled water.

Rinse: Night

- + other

ht: CH1

Ni

- + exit

Rinse Solution ?
exit

Waste Bottle OK?
exit

Rinse ChX YY sec
Sto

Soaking xx sec
Sto

es

es

es

es

Select and confirm Night Rinse procedure

Select required channel (1 - 4 or ALL)

Confirm rinsing solution and tube in bottle

Confirm waste bottle is empty

Wash system rinsed for set rinse time

The waste sink is filled with the distilled
water and the manifold is lowered into it

Manifold left in solution for set soak time (1 min)

Rinse: Auto

Rinse ChX YY sec
Sto

Soakin
Sto

Run XX:

- + other yes

Wash system rinsed for set rinse time

The waste sink is filled with the distilled water
and the manifold is lowered into it.

Manifold left in solution until Stop key pressed

Standby mode
Washing procedure can be performed

This procedure is the same as the Rinse: Day procedure, except that the rinsing
procedure is automatically repeated after a set repeat time. The repeat time can
be set in the Adjust menu between 1 and 24 hrs.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-5

3. Firmware and Software Description

3.2.4 Program

Selection of the programming procedure, to define the required program.

For more information about the programming procedure, see chapter 4 in

the Columbus Operating Manual.

3.2.5 Clear

This submenu is used to clear a program from the instrument's memory. If the
program is locked it can not be cleared and the following error message is
displayed:

Program locked
yes

The function Clear ALL is available within the Clear Program option. This
function enables you to clear all of the programs (excluding those that are
locked!). The Clear ALL function is found before program 1 and after program

30. Use the cursor keys to skip through the programs and once Clear ALL is
displayed press yes and all of the programs (with the exception of those that are
locked) will be cleared.

This option is not to be confused with the Clear ALL function in the Service
menu.

3.2.6 Show

Procedure to show the parameters for a program.

Clear XX:yyyyyyy

- + other yes

Clear XX:yyyyyyy

no yes

Clear XX:yyyyyyy

- + other yes

Select required program to be
cleared from instrument's memory

Confirm that selected program is
to be cleared

Select next program to be cleared or
required option in Mode Selection menu

For more information about the Show procedure, see Columbus Operating

Manual.

3-6 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

3.2.7 Adjust

The Adjust menu has the following options:

Plate Type

Prime Time

Rinse Time

Autorinse
Kit Select
Date
Time
Sel. Manifold
Adj. Liq. Sensor

Exit Adjust

Entering the Adjust Menu

When the instrument is in the standby mode and the following message is
displayed:

Run XX yyyyyyyy

- + other yes

Adjustment procedure for setting the parameters for up
to nine different types of microplates

Adjust the time that the Priming procedure is performed
for.

Adjust the time that the Rinsing procedure is performed
for.

Adjust the time between the Autorinse cycles.
This option is not available in this instrument.
Set the date
Set the time
Set the manifold that is fitted to the instrument
The switch thresholds for the wash, rinse and waste

liquid can be defined.
Exit from the Adjust menu.

To select the Adjust menu, press other u ntil the following message is displayed:
Adjust Menu

other yes
Select yes to enter the Adjust menu and then the various options in the Adjust

menu can now be selected using other.
When the required option is displayed, press yes to enter the parameter setting

procedure.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-7

3. Firmware and Software Description

A

A

Adjust Menu

other yes

Platetype: 1

- + other yes

Primetime:10 sec

- + other yes

Rinsetime: 10sec

- + other yes

AutoRinse: 1 h

- + other yes

Kit Select: Off

- + other yes

Confirm selection of Adjust Menu

Plate Type definition menu

Use '-' and '+' keys to select required p latet ype

Plate Type definition procedure

Priming Time setting

Use '-' and '+' keys to select required priming time
(5 - 99 sec.)*

Rinse Time setting

Use '-' and '+' key s to select required rinsing time
(5 - 99 sec.)*

utomatic Rinsing Time setting

Use '-' and '+' keys to select required tim e between

utomatic Rinsing procedures (1 - 24 hrs.)

This option is not available in this instrument

Date: ddmmmyyyy
d m other y

Time: hh:mm xx
h m other form

Sel.Manifold: xx

- + other yes

Adj.Liq.Sens.:
other yes

Date Setting

Use 'd' key to change day setting
Use 'm' key to chang month setting
Use 'y' key to change year setting

Time Setting

Use 'h' key to change hour setting
Use 'm' key to chang minute setting
Use 'Form ' key to change from 12 to 24 hrs format

Manifold setting

Use '-' and '+' keys to select fitted manifold . (8, 12, 16)
If Manifold detect option fitted, this setting is used
as the default when programming

Liquid Detection Sensor setting

Exit Adjust
other yes

Exit Adjust Menu

* Instruments that have the prime valve have a priming time of 8 - 99 seconds.

3-8 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02