tecan Columbus Washer Technical Manual

Technical Manual for

Columbus Washer

Document Part No.: 30000294

2006-02

Document Revision No.: 1.7

Firmware Level: 4.xx

TECAN Affiliates and Service Centers

Austria

Tecan Austria GmbH

Untersbergstrasse 1a
A-5082 Grödig/Salzburg

Austria

T [phone]
F [phone]
[e-mail]

Belgium

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Netherlands

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Theale
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China

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Switzerland

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USA

Tecan Systems Inc.

2450 Zanker Road
San Jose, CA 95131

USA

T [phone]
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Toll Free US/Canada:
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[e-mail]

2 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

WARNING

CAREFULLY READ AND FOLLOW THE INSTRUCTIONS

PROVIDED IN THIS MANUAL BEFORE OPERATING THE

INSTRUMENT.

Notice

Every effort has been made to avoid errors in text and diagrams, however, Tecan
Austria GmbH assumes no responsibility for any errors whi ch may appe ar in this
publication.

It is the policy of Tecan Austria GmbH to improve products as new techniques
and components become available. Tecan Austria GmbH therefor e reserves
the right to change specifications at any time.

We would appreciate any comments on this publication.
Tecan Austria GmbH
Untersbergstraße 1A
A-5082 Grödig/Salzburg
AUSTRIA / EUROPE
Telephone: [phone]
FAX: [phone]
E-mail: [e-mail]
www.tecan.com

Copyright Information

The contents of this manual are the property of Tecan Austria GmbH and are not
to be copied, reproduced or transferred to another person or persons without our
prior written permission.

Copyright © Tecan Austria GmbH
All rights reserved.
Printed in Austria.

Declaration for EU Certificate

See the back of this manual.

About this Manual

This technical manual describes the servicing of the Columbus Washer, designed
to wash microplates. It is intended only for service personnel.

This manual instructs how to:

• Install the instrument

• Operate the instrument

• Programming the wash procedure

• Definition on the wash parameters

• Cleaning and maintenance procedures

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3

Warnings, Cautions and Notes

There are three types of informational notices used in this manual. These notices
highlight important information or warn the user of a potentially dangerous
situation. The following notices are:

Note

Gives helpful information.

Caution

Indicates a possibility of instrument damage or data loss if instructions are

INDICATES THE POSSIBILITY OF SEVERE PERSONAL INJURY, LOSS OF

LIFE OR EQUIPMENT DAMAGE IF THE INSTRUCTIONS ARE NOT

not followed.

WARNING

FOLLOWED.

4 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

Table of Contents

1. General

1.1 Overview......................................................................................... 1-1

1.2 Instrument Description .................................................................1-2

1.2.1 Rear Panel Connections.................................................................. 1-2

1.2.2 Connection Diagram for Columbus (1 Liquid Channel)*.................. 1-4

1.2.3 Connection Diagram for Columbus (4 Liquid Channels)*................ 1-5

1.2.4 Keyboard and Display Unit..............................................................1-6

1.3 Interconnection Diagram .............................................................. 1-7

2. Installation Procedure

2.1 Unpacking and Inspection............................................................ 2-1

2.2 Unpacking Procedure ................................................................... 2-2

2.3 Power Requirements..................................................................... 2-2

2.4 Installation Procedure................................................................... 2-3

2.4.1 Installing the Instrument .................................................................. 2-3

2.4.2 Connecting the Waste Tubing......................................................... 2-3

2.4.3 Connecting the Solution Tubes ....................................................... 2-4

2.4.4 Fitting and Connecting the Liquid Sensors (Optional).....................2-5

2.4.5 Connecting the Barcode Reader (Optional)..................................... 2-6

2.4.6 Connecting the Printer (Optional)....................................................2-6

2.4.7 Power Connections ......................................................................... 2-6

3. Firmwar e and S oftwar e De s cription

3.1 Instrument Software Features...................................................... 3-1

3.2 Onboard Software Menus............................................................. 3-2

3.2.1 Run..................................................................................................3-2

3.2.2 Prime............................................................................................... 3-2

3.2.3 Rinse ............................................................................................... 3-3

3.2.4 Program........................................................................................... 3-6

3.2.5 Clear................................................................................................3-6

3.2.6 Show................................................................................................ 3-6

3.2.7 Adjust............................................................................................... 3-7

3.2.8 Service Menu................................................................................. 3-24

3.3 External Software ........................................................................ 3-61

3.3.1 Columbus Computer Software ...................................................... 3-61

3.3.2 Firmware Update...........................................................................3-61

3.4 Serial Interface............................................................................. 3-62

3.4.1 Hardware Specifications................................................................ 3-62

3.4.2 Contents........................................................................................ 3-65

3.4.3 A. Protocol.....................................................................................3-67

3.4.4 Sequence Number......................................................................... 3-68

3.4.5 B. Commands - Overview.............................................................. 3-69

3.4.6 Commands - Description............................................................... 3-71

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 5

3.4.7 D. Examples for Remote Control - Command Sequences.............3-88

3.5 Description of Error Messages...................................................3-95

4. Removing and Re placing the Instruments Cover

4.1 Removal of Instruments Top Cover..............................................4-1

4.2 Replacing the Instruments Top Cover .........................................4-3

5. Liquid System

5.1 Introduction ....................................................................................5-1

5.1.1 Liquid System Diagram....................................................................5-2

5.1.2 Dispensing System Description........................................................5-3

5.2 Replacement Procedure................................................................5-4

5.2.1 Replacing the Dispensing Pump......................................................5-4

5.2.2 Replacing the Solution Solenoid Valves (Instruments with Pinch

Valves) .............................................................................................

5.2.3 Replacing the Solution Solenoid Valves...........................................5-7

5.2.4 Replacing the Dispense Solenoid Valve ..........................................5-8

5.2.5 Replacing the Aspirating Pump........................................................5-9

5.2.6 Replacing the Aerosol Pump..........................................................5-10

5-6

6. Mechanical System

6.1 Manifold Arm Movement System..................................................6-1

6.1.1 Description of Manifold Arm Movement Parts..................................6-2

6.1.2 Adjusting the Manifold......................................................................6-2

6.2 Plate Transport System.................................................................6-3

6.2.1 Plate Support ...................................................................................6-4

6.2.2 Leveling the Plate Support...............................................................6-5

6.2.3 Microplate Positioning System.........................................................6-7

6.3 Replacement Procedure................................................................6-8

6.3.1 Replacing the Manifold Arm Error Sensor........................................6-8

6.3.2 Replacing the Motor.........................................................................6-9

6.3.3 Replacing the Top Position Optical Switch.....................................6-10

6.3.4 Replacing the Plate Support Home Position Optical Switch ..........6-11

7. Electronic System

7.1 Introduction ....................................................................................7-1

7.2 Interconnection Diagram...............................................................7-2

7.3 Main Board......................................................................................7-3

7.3.1 Replacing the Main Board................................................................7-4

7.3.2 Replacing the Battery.......................................................................7-5

7.4 Power Supply Board......................................................................7-6

7.4.1 Replacing the Power Supply Board..................................................7-7

7.5 Keyboard and Display Unit............................................................7-8

7.5.1 Replacing the Keyboard or Display Unit...........................................7-9

7.6 Manifold Detect Board.................................................................7-11

7.6.1 Replacing the Manifold Detect Board.............................................7-11

6 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

7.7 Fill Verification Board ................................................................. 7-13

7.7.1 Replacing the Fill Verification Board.............................................. 7-13

7.8 Optical Switches.......................................................................... 7-15

8. Serial Interface

8.1 Hardware Specifications............................................................... 8-1

8.1.1 Pin Designation ............................................................................... 8-1

8.1.2 RS-232-C Interface Lines................................................................8-2

8.1.3 Synchronization and Data Format...................................................8-3

8.1.4 Daisy Chaining Instruments............................................................. 8-3

9. Description of Options

9.1 Instrument Options ....................................................................... 9-1

9.2 Liquid Detection Option................................................................ 9-3

9.3 Barcode Reader Option ................................................................ 9-4

9.4 Printer Option ................................................................................ 9-5

9.5 Manifold Detection Option............................................................ 9-6

9.6 Fill Verification Option .................................................................. 9-8

9.7 Aerosol Pump Option.................................................................... 9-8

9.8 Activating the Options .................................................................. 9-8

10. Maintenance

10.1 Cleaning Maintenance................................................................. 10-1

10.1.1 Cleaning the Manifold.................................................................... 10-1

10.1.2 Exchanging the Manifold ............................................................... 10-3

10.1.3 Exchanging the Plate Support.......................................................10-5

10.1.4 Instrument Disinfection..................................................................10-6

10.2 Preventive Maintenance Plan..................................................... 10-8

10.2.1 Daily............................................................................................... 10-8

10.2.2 Weekly........................................................................................... 10-8

10.2.3 Every Six Months........................................................................... 10-8

10.2.4 Yearly (Service Technician Required)...........................................10-8

10.3 Service Tools ............................................................................... 10-9

10.3.1 Adjustment Plate (Reference Plate).............................................. 10-9

10.4 Electrostatic Discharge Information........................................ 10-10

10.4.1 Generating Static......................................................................... 10-10

10.4.2 Preventing Electrostatic Damage to Equipment.......................... 10-11

10.4.3 Personal Grounding Methods...................................................... 10-11

11. Trouble Shooting

11.1 Introduction.................................................................................. 11-1

12. Instrument Specifications

12.1 Introduction.................................................................................. 12-1

Index

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 7

1. General

1. General

1.1 Overview

This is a technical manual for servicing the Columbus Washer.
The instrument is a microprocessor controlled washer, designed to wash

microplates.
The instrument can be fitted with either an 8, 12 or 16 way manifold or a 384 Well

Manifold.
The manifolds are fitted with one aspirating and one dispensing needle per well.
The instrument can store up to nine software user-defined plate types including

dispensing and aspirating positions as well as the shape of the plate.
The dispensing speed can be selected from five set speeds and dropwise.
These features allow the instrument to be used for all washing applications

including Cell Washing applications.

The instrument should only be opened by authorized personnel. Before the

instrument is opened the mains power cable must be disconnected from

Ensure that the instrument has been disinfected before it is serviced.

the mains power socket.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 1-1

1. General

r

r

(

)

A

(

)

1.2 Instrument Description

The diagram below shows the main components of the instrument:

Manifold Arm

Manifold

POWER

Power ON LED

Keyboa r d an d

Display Unit

Waste Sink

Plate Support

Guide Bar

1.2.1 Rear Panel Connections

The instrument has the following connections on the back panel.

Mains Power

Socket

Fuse

Compartment

ON/OFF

Switch

Hand held

Barcode

Reader

Bottle Holder with

Liquid Sensor for

each bottle

Printer Connecto

or

Serial RS 232 fo

Remote Control

Inlets for channels 1 - 4

erosol

Tubing

option

Waste
Tubing

red

1-2 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

1. General

C

A

(Instrument with Pinch Valves)

Mains Power

Socket

Fuse

ompartment

ON/OFF

Switch

Hand held

Barcode

Reader

Bottle Holder with

Liquid Sensor for

each bottle

Prime
valve

Printer Connector

or

Serial RS 232 for

Remote Control

Channel 1

Inlet

Channel 2

Inlet

Channel 3

Inlet

erosol

Tubing

(yellow)

Channel 4

All connected devices must be proved and listed with regulations
EN 60950, UL 1950 or CSA C22.2 No. 950 for Data Processing Devices

Inlet

Waste

Tubing

(red)

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 1-3

1. General

)

p

g

q

g

1.2.2 Connection Diagram for Columbus (1 Liquid Channel)*

Hand held
Barcode

Reader (option)

Prime
valve

Printer (option

or

Com

uter for

Remote Control

Marked

reen

grey

Wash

uid

Li

Bottle Holder with liquid

sensor for each bottle

reen

Rinse
solution

red

Inlet
filter

* Some of the instruments may include the prime valve.

Waste
Bottle

Marked

red

1-4 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

1. General

1.2.3 Connection Diagram for Columbus (4 Liquid Channels)*

Hand held Barcode

Reader (option)

Printer (option)

Computer for

Remote Control

Marked green

Inlet
filter

Wash

Liquid 1

Bottle Holders with liquid

sensor for each bottle

or

Marked grey

Rinse

solution

grey red

green

Marked
red

Waste
Bottle

Marked
blue

None Return

Valve

yellow

Marked
yellow

Foam
Bottle

purple

blue

Aerosol

Filter

Seperator

Water

Wash

Liquid 3

Marked
purple

Inlet
filter

Marked

blue

Wash

Liquid 2

* Some of the instruments may include the prime valve.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 1-5

1. General

r

1.2.4 Keyboard and Display Unit

The Washer is operated via a keyboard and a control panel consisting of a two
row, sixteen digit Liquid Crystal Display (LCD) unit.

The top row of the display unit is used for messages and the bottom row for the
key functions or options.

A Light Emitting Diode (LED) in the center of the letter "O" of the word "POWER"
indicates when the Washer is switched ON.

Key 1 Key 2 Key 3

Key 4

POWER

Powe
ON LED

1-6 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

1. General

A

A

1.3 Interconnection Diagram

The diagram below shows how the various parts of the instrument are
interconnected.

Dispensing Pump

spirating Pump

erosol Pump

Prime Valve

Plate Home Position
Optical Switch

Plate Transport

Motor

Manifold Arm

Orbital Shaking

Motor

*

Motor

Home Position
Optical Switch

Manifold Typeand
Plate MismatchBoard

Fill Verify and
Plate Mismatch
Board

Solenoid 4

Solenoid 3

Solenoid 2

Solenoid 1
Dispense

Solenoid

Liquid
Detection
System

Keyboard /
Display Unit

Printer / Serial
Connector

EEPROM

CPU

Barcode Reader

Power Supply Board

Fuses

Mains Input

ON/OFF Switch

* The Orbital Shaking option is not used.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 1-7

2. Installation Procedure

(op

)

A

p

2. Installation Procedure

2.1 Unpacking and Inspection

The instrument is shipped in one carton, which contains:

• Instrument

• Accessory package which contains:

Spare manifold cover with two screws and Allen key
Operating manual
Mains power cable
Cleaning needles
2 spare fuses
Solution tube
3 spare 0 – ring seals for manifold

• Bottle set containing:

3 x 2.5 l bottles for the standard instrument

Liquid Sensor System

tional

Bottles

ccessory package

and o

erating manual

Instrument

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2-1

2. Installation Procedure

2.2 Unpacking Procedure

1. Visually inspect the container for damage before it is opened.

Report any damage immediately.

2. To place the instrument, select a location that is flat, level, vibration free,
away from direct sunlight, and free from dust, solvents and acid vapors.
Allow 10 cm distance between the back of the instrument and the wall or any
other equipment.

3. Place the carton in an upright position and open it.

4. Lift the instrument out of the carton and place it in the selected location.

5. Visually inspect the instrument for loose, bent or broken parts.

Report any damage immediately.

6. Compare the serial number on the rear panel of the instrument with the
serial number on the packing slip.

Report any discrepancy immediately.

7. Check the instrument accessories against the packing list.

8. Save packing materials for future use.

2.3 Power Requirements

The instrument is designed to operate at either 100 - 120 volt or 220 - 240 volt.

No voltage setting is required as the washer automatically senses the supplied
voltage.

The washer requires 2 x T 1.6 A / 250 V (slow blow) fuses.

WARNING

TO PREVENT THE RISK OF FIRE, THE MAINS FUSES SHOULD ONLY BE

REPLACED WITH THE SAME TYPE AND RATING OF FUSE.

2-2 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

2. Installation Procedure

A

2.4 Installation Procedure

Before the instrument is installed and switched on, it should be left to stand

for at least three hours, so there is no possibility of condensation causing a

2.4.1 Installing the Instrument

• Unpack the instrument and check the contents.

See 2.2 Unpacking Procedure.

• Connect the solution tubes to the inlet connectors.

See 2.4.3 Connecting the Solution Tubes.

• Setup possible instrument options. See page 2 – 5 and 2 – 6.

• Connect instrument to the power supply. See 2.4.7 Power Connections.

• Define the plate parameters in the Adjust menu. See chapter 3.

short circuit.

• Program the wash procedure. See chapter 3.

2.4.2 Connecting the Waste Tubing

Turn the instrument onto the right hand side.
Slide the waste tube through the hole in the back panel.

erosol Tube Hole

(optional)

Connect the waste tube onto the top connector of the aspirating pump.

Waste Tube Hole

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2-3

2. Installation Procedure

A

A

A

A

A

Standard Instrument

Waste Bottle

Dispensing

Pump

Waste Tube

spirating Tube to

Solution Tubes to Manifold

Aspirating Pump

Turn the instrument back to the normal position.
Place the other end of the waste tubing into the waste bottle.
Connect the aerosol tube onto the aerosol pump if this option is fitted. This is not

necessary for the standard instrument.
Instrument with Aerosol Protection Option

Waste Bottle

Dispensing Pump

erosol Bottle

Aspirating Tube to Manifold

Solution Tubes to Manifold

spirating Pump

erosol

Pump

Turn the instrument back to the normal position.
Place the other end of the aerosol tubing into the aerosol bottle.

2.4.3 Connecting the Solution Tubes

The solution tubes are connected onto the inlet connectors on the back panel of
the instrument. The instrument is supplied with four meters of tubing that can be
cut to the required length (maximum 1 meter).

Inlets for channe ls 1 - 4

erosol Tube*

(optional)

Waste Tube

red

2-4 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

2. Installation Procedure

2.4.4 Fitting and Connecting the Liquid Sensors (Optional)

Connect the detector unit cable into the appropriate socket in the back panel of
the instrument. The liquid sensor system can be extended to control up to four
different liquid bottles and one waste bottle.

rinse

grey

liquid 1

green

Liquid Sensor

Fix the tube support in the bottle holder as shown below.

Tube support

waste

red

Insert the tubes and connect the bottle holder to the instrument.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2-5

2. Installation Procedure

2.4.5 Connecting the Barcode Reader (Optional)

Before the barcode reader can be connected to the instrument, the supplied
barcode chips must be added to the main board. For more details contact your
service technician.

Hand Held Barcode

Reader

Liquid Sensor

2.4.6 Connecting the Printer (Optional)

Before the printer can be connected to the instrument, the supplied chip must be
added to the main board. For more details contact your service technician.

Connect the printer to the instrument using the supplied cable.
Connect the power supply cable to the printer.

ON /OFF

Switch

OFF 1 2

POWER

Connection Cable

BATT. LOW

POWER

ON

PAPER

FEED

Power Cable

2.4.7 Power Connections

Before the instrument is installed and switched on, it should be left to

stand for at least three hours, so there is no possibility of condensation

Ensure that the on / off switch in the back panel of the instrument is in the off
position.

Insert the mains power cable into the mains power socket on the rear of the
instrument.

2-6 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

causing a short circuit.

3. Firmware and Software Description

3. Firmware and Software
Description

3.1 Instrument Software Features

The instrument has the following software features:

• 30 user definable washing programs, permanently stored under the
numbers 1 to 30.

• Up to 60 processing steps per program, each processing step can be
identical or different from the previous step.

• 3 washing modes, normal washing, bottom washing and overflow washing.
Set by the Plate Type parameters.

• Soak time. selectable from 1 to 999 seconds.

• Software selectable dispensing speeds

• Software selectable aspirating speeds

• Software selectable bottom position, for aspiration contact to the bottom

of the wells.

• 4 rinse modes, the different rinse modes can be used to select how the
instrument is rinsed before it is left to stand or switched OFF.

• Automatic microplate centering, the microplate is automatically centered
before the start of the washing program.

• Programmable strip selection, the program can be defined so that the
strips to be washed can be selected before starting the washing procedure.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-1

3. Firmware and Software Description

3.2 Onboard Software Menus

The instrument can be used in the following onboard software menus.

3.2.1 Run

Selection of the run procedure to perform the selected program.

3.2.2 Prime

Before the instrument can be used, all dispensing channels must be primed
with the required solution.

Ensure that the bottle of priming solution is always full at the beginning of
the priming procedure and check that the inlet filters in the liquid tubes are
clear.

Please ensure that the dispensing and aspiration pumps are not run for

longer than a few minutes without liquid or they will be damaged.

The priming procedure is performed using the following procedure:

Run XX yyyyyyyy

- + other yes

Prime Ch1

- + other yes

Prime Solution ?
exit yes

Waste Bottle OK?
exit yes

Prime ChX Y sec
Stop

Priming Procedure

performed

Run XX yyyyyyyy

- + other yes

Select required channel to be primed

Required solution in bottle and inlet
tube inserted into solution

Waste bottle empty

Priming procedure being performed

Instrument ready to wash microplate

Caution

Do not use the instrument to aspirate or dispense any acidic solutions as

3-2 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

this could damage the instrument.

3. Firmware and Software Description

y

y

y

y

g

p

yyyyyyy

3.2.3 Rinse

There are four Rinse modes to flush the liquid system and prevent needle
blockages. They should be carried out when the instrument is left to stand or is
switched off at the end of working.

The Rinse modes are:

Rinse Soak
Rinse Day
Rinse Night
Rinse Auto

Rinse: Soak

This procedure is used if the instrument is to be left to stand for a short time.
(1/2 hr. or less).

With this procedure you can use either distilled water or wash solution.

Rinse: Soak

- + other

Soak: CH1

- + exit

Rinse Solution ?
exit

Waste Bottle OK?
exit

Soakin
Sto

Run XX:

- + other yes

es

es

es

es

Select and confirm Soak Rinse procedure

Select required channel (1 - 4)

Confirm that the rinsing solution
and the tube are in the bottle

Confirm waste bottle is empty

The waste sink is filled with solution
and the manifold is lowered into it

Manifold left in solution until Stop key pressed

Standby mode
Washing procedure can be performed

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-3

3. Firmware and Software Description

Rinse: Day

This procedure is used to rinse the wash solution system if the instrument is to be
left standing for a longer time. Alternatively it can be used to rinse the wash
solution system after the instrument has been left for a long time.

With this procedure you can use either distilled water or wash solution.
If the instrument is left to stand for 1 hr to 1/2 day, wash solution can be used, for

longer times use distilled water.

Rinse: Day

- + other yes

Day: CH1

- + exit yes

Rinse Solution ?
exit yes

Waste Bottle OK?
exit yes

Rinse ChX YY sec
Stop

Select and confirm Day Rinse procedure

Select required channel (1 - 4)

Confirm rinsing solution and tube in bottle

Confirm waste bottle is empty

Wash system rinsed for set rinse time

The waste sink is filled with solution and
the manifold is lowered into it

Soaking
Stop

Run XX:yyyyyyy

- + other yes

Manifold left in solution until

key pressed

Stop

Standby mode
Washing Procedure can be performed

3-4 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

Rinse: Night

y

g

y

y

y

p

p

g

p

yyyyyyy

p

3. Firmware and Software Description

This procedure is used to thoroughly rinse the wash solution system and leave
the manifold soaking in the waste sink full of solution at the end of working. This
procedure requires distilled water.

Rinse: Night

- + other

ht: CH1

Ni

- + exit

Rinse Solution ?
exit

Waste Bottle OK?
exit

Rinse ChX YY sec
Sto

Soaking xx sec
Sto

es

es

es

es

Select and confirm Night Rinse procedure

Select required channel (1 - 4 or ALL)

Confirm rinsing solution and tube in bottle

Confirm waste bottle is empty

Wash system rinsed for set rinse time

The waste sink is filled with the distilled
water and the manifold is lowered into it

Manifold left in solution for set soak time (1 min)

Rinse: Auto

Rinse ChX YY sec
Sto

Soakin
Sto

Run XX:

- + other yes

Wash system rinsed for set rinse time

The waste sink is filled with the distilled water
and the manifold is lowered into it.

Manifold left in solution until Stop key pressed

Standby mode
Washing procedure can be performed

This procedure is the same as the Rinse: Day procedure, except that the rinsing
procedure is automatically repeated after a set repeat time. The repeat time can
be set in the Adjust menu between 1 and 24 hrs.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-5

3. Firmware and Software Description

3.2.4 Program

Selection of the programming procedure, to define the required program.

For more information about the programming procedure, see chapter 4 in

the Columbus Operating Manual.

3.2.5 Clear

This submenu is used to clear a program from the instrument's memory. If the
program is locked it can not be cleared and the following error message is
displayed:

Program locked
yes

The function Clear ALL is available within the Clear Program option. This
function enables you to clear all of the programs (excluding those that are
locked!). The Clear ALL function is found before program 1 and after program

30. Use the cursor keys to skip through the programs and once Clear ALL is
displayed press yes and all of the programs (with the exception of those that are
locked) will be cleared.

This option is not to be confused with the Clear ALL function in the Service
menu.

3.2.6 Show

Procedure to show the parameters for a program.

Clear XX:yyyyyyy

- + other yes

Clear XX:yyyyyyy

no yes

Clear XX:yyyyyyy

- + other yes

Select required program to be
cleared from instrument's memory

Confirm that selected program is
to be cleared

Select next program to be cleared or
required option in Mode Selection menu

For more information about the Show procedure, see Columbus Operating

Manual.

3-6 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

3.2.7 Adjust

The Adjust menu has the following options:

Plate Type

Prime Time

Rinse Time

Autorinse
Kit Select
Date
Time
Sel. Manifold
Adj. Liq. Sensor

Exit Adjust

Entering the Adjust Menu

When the instrument is in the standby mode and the following message is
displayed:

Run XX yyyyyyyy

- + other yes

Adjustment procedure for setting the parameters for up
to nine different types of microplates

Adjust the time that the Priming procedure is performed
for.

Adjust the time that the Rinsing procedure is performed
for.

Adjust the time between the Autorinse cycles.
This option is not available in this instrument.
Set the date
Set the time
Set the manifold that is fitted to the instrument
The switch thresholds for the wash, rinse and waste

liquid can be defined.
Exit from the Adjust menu.

To select the Adjust menu, press other u ntil the following message is displayed:
Adjust Menu

other yes
Select yes to enter the Adjust menu and then the various options in the Adjust

menu can now be selected using other.
When the required option is displayed, press yes to enter the parameter setting

procedure.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-7

3. Firmware and Software Description

A

A

Adjust Menu

other yes

Platetype: 1

- + other yes

Primetime:10 sec

- + other yes

Rinsetime: 10sec

- + other yes

AutoRinse: 1 h

- + other yes

Kit Select: Off

- + other yes

Confirm selection of Adjust Menu

Plate Type definition menu

Use '-' and '+' keys to select required p latet ype

Plate Type definition procedure

Priming Time setting

Use '-' and '+' keys to select required priming time
(5 - 99 sec.)*

Rinse Time setting

Use '-' and '+' key s to select required rinsing time
(5 - 99 sec.)*

utomatic Rinsing Time setting

Use '-' and '+' keys to select required tim e between

utomatic Rinsing procedures (1 - 24 hrs.)

This option is not available in this instrument

Date: ddmmmyyyy
d m other y

Time: hh:mm xx
h m other form

Sel.Manifold: xx

- + other yes

Adj.Liq.Sens.:
other yes

Date Setting

Use 'd' key to change day setting
Use 'm' key to chang month setting
Use 'y' key to change year setting

Time Setting

Use 'h' key to change hour setting
Use 'm' key to chang minute setting
Use 'Form ' key to change from 12 to 24 hrs format

Manifold setting

Use '-' and '+' keys to select fitted manifold . (8, 12, 16)
If Manifold detect option fitted, this setting is used
as the default when programming

Liquid Detection Sensor setting

Exit Adjust
other yes

Exit Adjust Menu

* Instruments that have the prime valve have a priming time of 8 - 99 seconds.

3-8 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

Adjust Plate Type

This option is used to set the following parameters for the different types of
microplates. The default is a 96 well Greiner microplate.

The following parameters are set:

Parameters Options

Plate type number
Bottom Form

Dispensing Position

Aspirate Position

Aspirate Position 2

Bottom Position

Bottom Wash Position

Overflow Wash
Position

Before starting the adjustment procedure, insert the microplate to be
adapted into the plate support.

1 - 9
Set whether the microplate has flat or round

bottom wells.
Set the position in the wells where the solution

will be dispensed.
Set the position in the wells where the solution

will be aspirated.
Set the position in the wells for the crosswise

aspiration procedure. (flat bottom wells only)
Set the position of the aspirating needles for the

bottom of the well.
Set the position of the aspirating needles for the

bottom washing procedure.
Set the position of the aspirating needles for the

overflow washing procedure.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-9

3. Firmware and Software Description

Round Bottom Plates

Adjust Menu
other yes

Plate: X

- + other yes

Plate: X
890 , , ABCDE

Bottomform Round

- + exit yes

Disp.Pos XXX

- + exit yes

Asp.Pos XXX

- + exit yes

Confirm selection of Adjust menu

Plate Type definition menu

Use '-' and '+' keys to select required platetype (1 - 9)

Enter the required name for the plate
(up to 8 characters). 1,2,3,4,5,6,7,8,9,0 & A-Z
To enter a name use the ‘<’ and ‘>’ keys to place a character
between the commas. Confirm the selection with the ‘↵’ key.
To delete the last character use the ‘ ’ sign.
When the name is complete, confirm with the ‘↵’ key.

Plate Bottom Form setting

Use '-' and '+' keys to select required bottom form
(flat or round)

Set Dispensing position

Use '-' and '+' keys to move the plate support so
that the dispensing needles are in the correct position

Set Aspirating position

Use '-' and '+' keys to move the plate support so
that the aspirating needles are in the correct position

Asp.Pos Botm XXX

- + exit yes

Washpos Botm XXX

- + exit yes

Washpos Ovfl XXX

- + exit yes

Platetype: X

- + other yes

Use '-' and '+' keys to move the manifold so that the
aspirating needles are in the correct position for
aspirating the bottom of the wells

Set Bottom Washing position

Use '-' and '+' keys to move the manifold so that the
aspirating needles are in the correct position for
the Bottom Washing procedure

Set Overflow Washing position

Use '-' and '+' keys to move the manifold so that the
aspirating needles are in the correct position for
the Overflow Washing procedure

Select plate type to be defined

Set Bottom Aspirating position

3-10 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

Flat Bottom Plates

Adjust Menu
other yes

Plate: X

- + other yes

Plate: X
890 , , ABCDE

Bottomform Flat

- + exit yes

Disp.Pos XXX

- + exit yes

Asp.Pos 1 XXX

- + exit yes

Confirm selection of Adjust menu

Plate Type definition menu

Use '-' and '+' keys to select required platetype (1 - 9)

Enter the required name for the plate
(up to 8 characters). 1,2,3,4,5,6,7,8,9,0 & A-Z
To enter a name use the ‘<’ and ‘>’ keys to place a character
between the commas. Confirm the selection with the ‘↵’ key.
To delete the last character use the ‘ ’ sign.
When the name is complete, confirm with the ‘↵ ’ key.

Plate Bottom Form setting

Use '-' and '+' keys to select required bottom form
(flat or round)

Set Dispensing position

Use '-' and '+' keys to move the plate support so
that the dispensing needles are in the correct position

Set Aspirating position 1

Use '-' and '+' keys to move the plate support so
that the aspirating needles are in the correct position

Asp.Pos 2 XXX

- + exit yes

Asp.Pos Botm XXX

- + exit yes

Washpos Botm XXX

- + exit yes

Washpos Ovfl XXX

- + exit yes

Platetype: X

- + other yes

Set Aspirating position 2

Use '-' and '+' keys to move the plate support so
that the aspirating needles are in the correct position

Set Bottom Aspirating position

Use '-' and '+' keys to move the manifold so that the
aspirating needles are in the correct position for
aspirating the bottom of the wells

Set Bottom Washing position

Use '-' and '+' keys to move the manifold so that the
aspirating needles are in the correct position for
the Bottom Washing procedure

Set Overflow Washing position

Use '-' and '+' keys to move the manifold so that the
aspirating needles are in the correct position for
the Overflow Washing procedure

Select Plate type to be defined

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-11

3. Firmware and Software Description

To enter the Plate type option, press other until the following message is
displayed:

Plate: XXXXX

- + other yes
Where X is the displayed plate type number and plate name.

Use the - and + keys to select the required plate type number that is to be
defined. (1 - 9).

Pressing - decreases the displayed plate type.
Pressing + increases the displayed plate type.
When the required plate type is displayed press yes and the following message is

displayed:
Bottomform Flat

- + exit yes
Use the - and + keys to select the required bottom form of the wells. (flat or

round).
When the required bottom form is displayed press yes to confirm the selection.

Flat Bottom Wells

The following procedure is used when a microplate with flat bottom wells is to be
defined.

After yes has been pressed to confirm the selection of flat bottom wells, the
instrument initializes the motors and moves the microplate under the manifold.

When the microplate is under the manifold, the following message is displayed:
Disp-Pos: XXX

+ exit yes
Where XXX is the displayed number of steps.

Use the - and + keys to move the microplate until the dispensing needles are in
the correct position.

Pressing the - and + keys moves the microplate forwards and backwards so that
the dispensing needles are positioned further back/forward in the wells.

When the dispensing needles are in the correct position, press yes and the
following message is displayed:

Asp.Pos 1 XXX

- + exit yes

3-12 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

Use the - and + keys to move the microplate until the aspirating needles are in
the correct position for aspirating the side of the well.

Pressing the - and + keys moves the microplate forwards and backwards so that
the aspirating needles are positioned further back/forward in the wells.

When the aspirating needles are in the correct position, press yes and the
following message is displayed:

Asp.Pos 2 XXX

- + exit yes
Use the - and + keys to move the microplate until the aspirating needles are in

the correct position for aspirating the side of the well. (crosswise aspiration)

Pressing the - and + keys moves the microplate forwards and backwards so that
the aspirating needles are positioned further back/forward in the wells.

When the aspirating needles are in the correct position press yes and the
following message is displayed:

Pos Botm XXX

Asp.

- + exit yes
Use the - and + keys to move the manifold until the aspirating needles are in the

correct position for aspirating the bottom of the well.

Pressing the - and + keys move the manifold up and down so that the aspirating
needles are positioned higher/lower in the wells.

When the aspirating needles are in the correct position, press yes and the
following message is displayed:

Washpos Botm XXX

- + exit yes

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-13

3. Firmware and Software Description

Use the - and + keys to move the manifold until the aspirating needles are in the
correct position for aspirating the solution in the bottom washing position.

Pressing the - and + keys moves the manifold up and down so that the aspirating
needles are positioned higher/lower in the wells.

When the aspirating needles are in the correct position, press yes and the
following message is displayed:

Washpos Ovfl XXX

- + exit yes
Use the - and + keys to move the manifold until the aspirating needles are in the

correct position for aspirating the solution in the overflow washing position.

Pressing the - and + keys move the manifold up and down so that the aspirating
needles are positioned higher/lower in the wells.

When the aspirating needles are in the correct position, press yes and the
following message is displayed:

Platetype: X

- + other yes
The parameters for the next plate type can be defined or press other for the next

option in the Adjust menu.

3-14 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

Round Bottom Wells

The following procedure is used when a microplate with round bottom well s is to
be defined.

After yes has been pressed to confirm the selection of round bottom wells, the
instrument initializes the motors and moves the microplate under the manifold.

When the microplate is under the manifold, the following message is displayed:
Disp.Pos XXX

- + exit yes
Where XXX is the displayed number of steps.

Use the - and + keys to move the microplate until the dispensing needles are in
the correct position.

Pressing the - and + keys moves the microplate forwards and backwards so that
the dispensing needles are positioned further back/forward in the wells.

When the dispensing needles are in the correct position, press yes and the
following message is displayed:

Asp.Pos XXX

- + exit yes
Use the - and + keys to move the microplate until the aspirating needles are in

the correct position for aspirating the middle of the well.

Pressing the - and + keys moves the microplate forwards and backwards so that
the aspirating needles are positioned further back/forward in the wells.

When the aspirating needles are in the correct position, press yes and the
following message is displayed:

Asp.Pos Botm XXX

- + exit yes

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-15

3. Firmware and Software Description

Use the - and + keys to move the manifold until the aspirating needles are in the
correct position for aspirating the bottom of the well.

Pressing the - and + keys moves the manifold up and down so that the aspirating
needles are positioned higher/lower in the wells.

When the aspirating needles are in the correct position, press yes and the
following message is displayed:

Washpos Botm XXX

- + exit yes
Where XXX is the displayed number of steps.

Use the - and + keys to move the manifold until the aspirating needles are in the
correct position for aspirating the solution in the bottom washing position.

Pressing the - and + keys moves the manifold up and down so that the aspirating
needles are positioned higher/lower in the wells.

When the aspirating needles are in the correct position, press yes and the
following message is displayed:

Washpos Ovfl XXX

- + exit yes
Use the - and + keys to move the manifold until the aspirating needles are in the

correct position for aspirating the solution in the overflow washing position.

Pressing the - and + keys moves the manifold up and down so that the aspirating
needles are positioned higher/lower in the wells.

When the aspirating needles are in the correct position, press yes and the
following message is displayed:

Platetype: X

- + exit yes
The parameters for the next plate type can be defined or press other for the next

option in the Adjust menu.

3-16 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

Adjust Prime Time

This option is used to set the time that the Prime procedure is performed for.

Adjust Rinse Time

Primetime:10 sec

+ - other yes

Rinsetime:10 sec

- + other yes

Use '-' and '+' keys to select required priming
time (5- 99 seconds)*

Adjust menu

other yes

To enter the Prime Time option, press other until the following message is
displayed:

Primetime:10 sec

- + other yes
Use the - and + keys select the required priming time in seconds. (5 - 99 sec)*

When the required prime time is displayed, either press other to proceed to the
next option or press yes to exit from the Adjust menu.

This option is used to set the time that the rinse procedure is performed for.

Rinsetime:10 sec

+ - other yes

Autorinse:1 h

- + other yes

Use '-' and '+' keys to select required rinsing
time (5 - 99 seconds)*

Adjust menu

other yes

To enter the Rinse Time option, press other until the following message is
displayed:

Rinsetime:10 sec

- + exit yes
Use the - and + keys select the required rinse time in seconds. (5 - 99 sec)*

When the required rinse time is displayed, either press other to proceed to the
next option or press yes to exit from the Adjust menu.

* Instruments that have the prime valve have a priming time of 8 - 99 seconds.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-17

3. Firmware and Software Description

h

Adjust Auto Rinse

This option is used to set the time between the rinse procedures when the
Autorinse mode is selected.

Kit Select

AutoRinse: 1h

+ - other yes

Kit Select: Off

- + other yes

Use '-' and '+' keys to select required Auto
rinsing time (1 - 24 hours)

Adjust menu

other yes

To enter the AutoRinse Time option, press other until the following message is
displayed:

AutoRinse: 1 h

- + exit yes
Use the - and + keys select the required time in hours between each automatic

rinse procedure. (1 - 24 hours)
When the required time is displayed, either press other to proceed to the next

option or press yes to exit from the Adjust menu.

This option is not available in the instrument.

Date

The setting for the date can only be altered if the Printer option is fitted.

Date: dd mmm yyyy
d m other y

Time: hh:mm:ssXX
h m other form

Use the 'd' key (1st key) to set the required Day
Use the 'm' key (2nd key) to set the required Mont
Use the 'y' key (4th key) to set the required Year

To enter the Date option, press other until the following message is displayed:

Date:dd mmm yyyy

d m other y
Where dd mmm yyyy is the displayed date.

3-18 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

Time

y

3. Firmware and Software Description

Use the d, m and y to set the required date.
Pressing d increases the displayed day setting
Pressing m increases the displayed month setting
Pressing y increases the displayed year setting
When the required date is displayed, press other to confirm the setting and the

following message is displayed:

Time: hh:mm:ssXX
h m other form

The setting for the time can only be altered if the Printer option is fitted.

Time: hh:mm:ssXX
h m other form

Sel.Manifold: XX

- + other

es

Use the 'h' key (1st key) to set the required Hour
Use the 'm' key (2nd key) to set the required Minute
Use the 'Form' key (4th key) to set the required form
AM/PM or 24 hr.

To enter the Time option, press other until the following message is displayed:

Time: hh:mm:ssXX
h m other form

Where hh:mm:ss is the displayed time and XX is the time form (AM/PM).
Use the h, m and form to set the required time and format.
Pressing h increases the displayed hour setting
Pressing m increases the displayed minute setting
Pressing form changes the time format (a.m./p.m. or 24 hr.)
When the required time and format is displayed, press other to confirm the

setting and the following message is displayed:
Sel.Manifold: XX

- + other yes

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-19

3. Firmware and Software Description

Select Manifold

This option is used to set the type of manifold that is fitted.

This setting is used if neither the manifold detect option nor the fill
verification option are fitted.

Sel.Manifold: XX

- + other yes

Exit Adjust
other yes

Use the '-' key (1st key) and '+' (2nd key) to set
the setting for the manifold (8, 12 or 16 way)

Press 'yes' key (4th key) to confirm and save setting
Press 'other' key (3rd key) to move to next option
without saving setting

To enter the Select Manifold option, press other until the following message is
displayed:

Sel.Manifold: XX

- + other yes
Use the - and + keys to set the required manifold. (8, 12 or 16 way)

Press other to proceed to the next option without saving any changes in the
setting.

Press yes to confirm the setting and proceed to the next option.
When other or yes has been pressed, the following message is displayed:

Adjust Liq. Sens.:
other yes

3-20 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

y

Adjust Liquid Sensors

Please prepare the bottles before performing the procedure below. The bottles
marked green (wash solution) and gray (rinse solution) should be filled with
600ml of water and the bottle marked red (waste) should be filled with 2000 ml of
water. Place all three bottles on the corresponding colored bottle holders. Go to
the Adjust menu and select Adj. Liq. Sens. as described below:

Caution

This procedure must be performed when the instrument is installed.

Adj. Liq. Sens.:
other yes

Liq. Sens. 1

- + exit yes

Empty 1 XX 1

exit yes

Empty 1 XX 0
exit yes

Use the cursor keys to repeat this procedure
for the grey (rinse solution) and red (waste)
bottles. See also below for more details.

Press yes to start the adjustment procedure

Using the - and + keys select the liquid sensor to
be calibrated.
1 = Green (wash solution)
2 = Gray (rinse solution)
W = Red (waste)
Press yes

to continue the adjustment procedure.

Check that the adjustment of the magnet screw has
a blank value of between 5 and 20. See photo1.
XX = displayed sensor value (does not directly
correlate to the volume of liquid in the bottles).
1 = values above limit
Press

to confirm the limit.

es

XX = displayed value 0 = values below limit
Press

to continue the adjustment of other sensors.

exit

Photo 1

Check that the adjustment of the magnet
screw has a blank value of between
5 and 20

If it was necessary to readjust the
settings, secure the screws with
adhesive.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-21

3. Firmware and Software Description

Liquid Sensors 1 – 2 (3 – 4)

When a liquid sensor (1 - 2) has been selected, the following messa ge is
displayed:

Empty S XX Y
exit yes

Where S = the sensor number, XX is the displayed value and Y = limit
(0 = values below limit, 1 = values above limit)

Adjust the volume of solution in the bottle to the lowest volume that can be used
before starting the procedure.

When the minimal volume is in the bottle, press yes to confirm the value
(volume).
Green bottle (wash solution) = 600 ml
Gray bottle (rinse solution) = 600 ml

When the sensor detects a volume less than this entered volum e, it will display
an error message either before starting the test procedure or after the procedure
has been completed.

When the value has been set, press exit and the following message is displayed:
Liquid Sens. 1

- + exit yes
Use the < and > keys to set the next required sensor (1 – 2 or W)

Waste Sensor

When the waste sensor has been selected, the following message is displayed:
Full W XX Y

exit yes
Where W = Waste sensor, XXX is the displayed value and Y = limit

(0 = values below limit, 1 = values above limit)
Adjust the volume of solution in the waste bottle to the highest volume that can be

used before starting the procedure.
When the maximum volume is in the bottle, press yes to confirm the value

(volume).
Red bottle (waste) = 2000 ml

When the sensor detects a volume greater than this entered volume, it will
display an error message either before starting the test procedure or after the
procedure has been completed.

When all the sensors have been set, press exit and the following message is
displayed:

Adj.Liq.Sens.:
other yes

Press other to proceed to the next option.

3-22 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

Exit Adjust

3. Firmware and Software Description

This option is used to exit from the Adjust menu.

Exit Adjust
other yes

Platetype: 1

- + other yes

Select if the Adjust Menu is to be exited or not

Run X:yyyyyyy

- + other yes

When the following message is displayed:
Exit Adjust

other yes
Select if the Adjust menu is to be exited or not.

Remain in Adjust Menu

Press other to remain in the Adjust menu and the following message is
displayed:

Platetype: 1

- + other yes
Select the required option in the Adjust menu.

Exit Adjust Menu

Press yes and the following message is displayed:
Run X:yyyyyyy

- + other yes
The instrument is now in the Standby mode and can be used to wash a

microplate.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-23

3. Firmware and Software Description

3.2.8 Service Menu

The Service submenu has the following options:

Clear All ?

Prog Access

Adj. Access

Header

Channels

Skip Messages

Duration Test
Function Test
Select Options
Adj. Dispens. Pump
Adj. Prime Pos.

Adj. Plate Ref.

Procedure to clear all the defined programs and
instrument settings from the memory.

Procedure to select if access to the programming
procedure is allowed.

Procedure to select if access to the Adjust menu is
allowed.

Procedure for defining the start-up header for the
instrument.

Procedure for setting the number of dispensing
channels to be used.

Procedure to set if the reminder messages are to be
displayed.

Procedure for life testing the instrument.
Procedure for testing various instrument component.
Procedure for defining fitted instrument options.
Procedure for adjusting the dispensing pump.
Procedure for adjusting the position of the plate support

for the Prime / Rinse procedures. (Important adjustment
with 16 way manifold)

Procedure for adjusting the position of the manifold
needles.

3-24 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

V

A

q

Service
other yes

Code:
1 2 1 4 1 8

Clear All ?
yes exit no

Prog Access: On

- + exit yes

Adj. Access: On

- + exit yes

Header:
Set exit yes

Channels: X

- + exit yes

Skip Messages:
yes exit no

Enter required code '121418' (for firmware

4.20 and higher)

(Enter ‘1248’ for firmware V4.01 up to but not
including V4.20)

Clear All programs and settings from memory

ttention: This deletes parameter settings

which re

uire special tools to be redefined.

Select access to programming procedure

If access is off the programme menu is omitted
from the Mode Selection menu.

Select access to Adjust menu

If access is off the Adjust menu is omitted
from the Mode Selection menu.

Set startup header for instrument

Set number of dispensing channels fitted (1 - 4)

Select if warning message are to be skipped

Duration Test
yes exit no

Function Test
yes exit no

Select Options
yes exit no

Adj Dispens Pump
yes exit no

Adj.Prime Pos.
yes exit no

Service End
yes no

Standby Mode

Life testing procedure

Procedure for testing the various
instrument componants

Procedure for defining fitted instrument options

Procedure for adjusting dispensing pump

Procedure for adjusting the position of the
plate support for the Prime / Rinse procedures.

(Important adjustment with 16 way manifold)

Exit Service menu

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-25

3. Firmware and Software Description

Entering the Service Menu

The Service menu is entered using the following procedure:
When the instrument has been switched on, it is in the standby mode and

displays the message
Run XX yyyyyyyy

- + other yes
Where XX is the displayed program number and yyyyyyyy the program name.

To select the Service menu, press other until the following message is displayed:
Service

other yes
Press yes to enter the Service menu and the following message is displayed:
Code:

1 2 1 4 1 8

The Service submenu can only be entered by entering the required code.

Enter required code '121418' (for firmware V4.20 and higher)

(Enter ‘1248’ for firmware V4.01 up to but not including V4.20)

When the code has been correctly entered the first option of the Service menu is
displayed:

Clear ALL ?
yes exit no

3-26 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

Clear ALL

e

3. Firmware and Software Description

This option is used to clear ALL the defined programs and instrument settings
from the instrument's memory.

This deletes parameter settings, which require special tools to be

redefined.

Service
other yes

Clear All ?
yes exit no

Are you sure ?
exit yes

Initializing
please wait

Run 1:

- + other yes

Prog Access: On

- + exit yes

Clear All defined programs and settings

Confirm that All defined programs and
settings are to be cleared

Instrument re-initialized

Instrument in Standby Mode

Select if access to the programming procedur
is to be allowed or not.

When the following message is displayed:
Clear ALL ?

yes exit no
Select if all the programs and settings are to be cleared or not.

Press no if all the programs are not to be cleared, the instrument then proceeds
to the next option.

Press exit to exit from the Service menu.
Press yes to clear all the programs from the instrument's memory and the

following message is displayed:
Are you sure ?

exit yes

This message is displayed as a control to ensure that the programs and
settings are not accidentally cleared.

Press exit to return to the previous message.
Press yes to confirm that all the programs are to be cleared.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-27

3. Firmware and Software Description

A

After yes has been pressed, all the programs and settings are cleared from the
instrument's memory, the instrument is re-initialized and the following message is
displayed:

Run 1:

- + other yes
The instrument is now in the Standby mode.

Re-program the instrument for the required programs and settings.

Instrument options, dispensing pump calibration, priming position.
The following options must be re-calibrated: the dispensing pump calibration, the

liquid sensor and bottle management system and the X and Z offset value using
adjustment plate.

The following data must be reprogrammed using the WinWash software (see
software manual): test parameters, options and plate types.

Programming Access

This option is used to select if access to the programming procedure is permitted.

Service
other yes

Prog Access: On

- + exit yes

Prog Access: Off

- + exit yes

Adj. Access: On

- + exit yes

Use '-' and '+' key to select if access to
programming procedure is permitted.

Use '-' and '+' key to select if access to

djust menu is permitted

When the following message is displayed:
Prog Access: On

- + exit yes
Use the 1st and 2nd keys to select if access is permitted.

On = access permitted, Off = access NOT permitted
When the required setting is displayed, press yes and the following message is

displayed:
Adj. Access: On

- + exit yes
When the access is not permitted, the menu point Programme is omitted from the

Mode Selection menu.

3-28 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

A

Adjust Access

This option is used to select if access to the Adjust menu is permitted.

Service
other yes

Adj. Access: On

- + exit yes

Adj. Access: Off

- + exit yes

Header:
Set exit yes

Use '-' and '+' key to select if access to

djust Menu is permitted.

Procedure to define startup header

When the following message is displayed:
Adj. Access: On

- + exit yes
Use the 1stand 2nd keys to select if access is allowed or not.

On = Access allowed, Off = Access NOT allowed
When the required setting is displayed press yes and the following message is

displayed:
Header:

Set exit yes
When the access is not allowed, the Adjust menu is omitted from the Mode

Selection menu.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-29

3. Firmware and Software Description

Header

This option is used to define the start-up header for the instrument.

Service
other yes

Header:
Set exit yes

COLUMBUS

890

Channels: X

- + exit yes

ABCDE

↵

Select if header is to be altered or not

Edit or enter the required header

Select number of dispensing channels fitted

When the following message is displayed:
Header:

Set exit yes
Press Set to enter the header setting procedure.

Press yes to confirm the displayed header and proceed to the next option.
When Set has been pressed, the following message is displayed:

COLUMBUS
890 ABCDE

In the bottom row of the display are shown the characters that can be selected for
the header. A maximum of sixteen characters can be used.

The possible characters for the header are:
1234567890ABCDEFGHIJKLMNOPQRSTUVWXYZ

There are two special characters '
The character '

The character '

' is used to clear the last entered character from the header.
' is used to confirm that the completed header has been entered

' and ' ' .

and is used to exit from the header programming procedure.

st

To enter the required header, use the 1

and 2nd keys to scroll the characters

across the display.
When the required character is displayed between the two highlighters in the

middle of the display, press the 4

th

key. The character is then entered into the top

line of the display.

3-30 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

After the selected character has been entered, the instrument returns to the start
position with the '

WASHER
890 ABCDE

' character between highlighters. For example:

Channels

When the required header has been entered, the header programming procedure
is exited by pressing the 4

highlighters.
The following message is displayed:

Channels: X

- + exit yes

This option is used to select the number of dispensing channels that are fitted to
the instrument.

th

key when the ' ' character is between the two

Service
other yes

Channels:

- + exit yes

Skip
yes exit no

Use '-' and '+' keys to define the
of dispensing channels fitted (1 - 4)

Select if warning messages are to be
skipped or not.

When the following message is displayed:
Channels: X

- + exit yes
Use the 1st and 2nd keys to select the number of channels that are fitted and that

can be used. (1 - 4)
When the required setting is displayed, press yes and the following message is

displayed:
Skip Messages:

yes exit no

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-31

3. Firmware and Software Description

Skip Messages

This option is used to select if the warning messages are to be displayed or not.

Skip Messages:

yes exit no

Channel primed?
show exit skip

Plate inserted ?
show exit skip

Waste Bottle OK?
show exit skip

Rinse Solution ?
show exit skip

Prime Solution ?
show exit skip

Select if this message is to be displayed

Press 'show' key to display message

Press 'skip' key to omit message

Select if this message is to be displayed

Press 'show' key to display message

Press 'skip' key to omit message

Select if this message is to be displayed

Press 'show' key to display message

Press 'skip' key to omit message

Select if this message is to be displayed

Press 'show' key to display message

Press 'skip' key to omit message

Select if this message is to be displayed

Press 'show' key to display message

Press 'skip' key to omit message

You should Rinse

show exit skip

Skip Messages:
yes no

Service
other yes

Select if this message is to be displayed

Press 'show' key to display message

Press 'skip' key to omit message

Duration Test
yes exit no

3-32 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

When the following message is displayed:
Skip Messages:

yes exit no
Press no if the warning messages are not to be skipped. The instrument then

proceeds to the next option.
Press yes if the warning messages are to be skipped.
When yes is pressed, the following message is displayed:

Channel X primed?
show exit skip

Press show if this message is to be displayed.
Press skip if this message is not to be displayed.
When the key for the required selection has been pressed, the following message

is displayed:
Plate inserted ?

show exit skip
Press show or skip and the following message is displayed:
Waste Bottle OK?

show exit skip
Press show or skip and the following message is displayed:
Rinse Solution ?

show exit skip
Press show or skip and the following message is displayed:
Prime Solution ?

show exit skip
Press show or skip and the following message is displayed:
You Should Rinse

show exit skip
Press show or skip and the following message is displayed:
Skip Messages:

yes no
Press yes to exit from the Service menu.

Press no to proceed to the next option in the Service menu.
When no is pressed, the following message is displayed:

Duration Test
yes exit no

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-33

3. Firmware and Software Description

e

y)

F

Duration Test

This option is used to select the life test procedure for the instrument.
Before starting the Duration Test procedure, place a microplate into the plate

support.

Priming
procedure

Service
other yes

Duration T
yes exit no

C:XX h:mm:ss
clr exit cont

Run XX yyyyyyyy

- + other yes

Nr.of Strips: XX

- + exit yes

Channel X primed?
no yes

Plate inserted ?
exit yes

st

Duration Testing procedure

unction Test
yes exit no

Instrument displays values from last
Duration Test procedure

Press 'clr' to clear values
Press 'cont' to start procedure

Select required program

Select required number of strips

Required channel primed

Plate inserted

Waste Bottle OK?
exit yes

Duration Test

procedure started

C: X Yxxxxx
h:mm:ss

Is the waste bottle empty and
the waste tube connected?

Instrument displays number of cycles
performed, the type of step being performed
and the total time for the Duration Test.

Press 'Stop' key (3rd ke

to stop Duration Test

Run XX yyyyyyyy

- + other yes

Instrument in Standby Mode

3-34 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

When the following message is displayed:
Duration Test

yes exit no
Press no if the life test procedure is not to be performed. The instrument then

proceeds to the next option and the following message is displayed:
Function Test

yes exit no
Press yes to enter the life test procedure and the following message is displayed:
C:XX h:mm:ss

clr exit cont
This message displays the values from the last Duration Test procedure C:XX =

number of cycles performed, h:mm:ss = time that the procedure was perform ed
for.

Press clr to clear the values.
Press exit to return to the previous message.
Press cont to start the Duration Test procedure.
When cont is pressed, the following message is displayed:

Run 1:XXXXXXX

- + other yes
Use the - and + keys to select the required test that is to be used for the Duration

Test procedure. Any defined test procedure can be used.
When the required test procedure is displayed, press yes to confirm the selection.
The following message is displayed:

Nr of Strips: XX

- + exit yes
Use the - and + keys to select the required number of strips to be used (1 - 12).

When the required number of strips is displayed, press yes to confirm the
selection.

The following message is displayed:
Channel Xprimed?

no yes
Where X is the displayed channel number.

This optional message can be displayed as a reminder to ensure that the
instrument has been primed with the correct solutions.

If the required channel has not been primed, press no and the instrument
automatically exits from the Run mode and enters the priming procedure.

If the required channel has been primed, press yes and the following message is
displayed:

Plate inserted ?
exit yes

This optional message can be displayed as a reminder to ensure that the
microplate has been inserted into the plate support.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-35

3. Firmware and Software Description

When the microplate has been inserted into the plate support, press yes and the
following message is displayed:

Waste Bottle OK?
exit yes

This optional message can be displayed as a reminder to ensure that the waste
bottle is empty and that the waste tube is in the waste bottle.

This message is not displayed if Waste Full Sensor is fitted.
Press yes to confirm that the waste bottle is empty and the waste tube is inserted

into the waste bottle and the instrument then starts the Duration Test procedure.
While the instrument is performing the Duration Test procedure, messages are

displayed giving information about the procedure. For example:
C:XX Yxxxx

h:mm:ss
Where C:XX = number of cycles performed, Yxxxx = the type of program step

that is being performed and h:mm:ss = the time that the Duration Test has been
performed for.

The life test procedure is repeated until the Stop key is pressed.

To stop the life test procedure, press Stop and the instrument then returns to the
standby mode and the following message is displayed:

Run XX yyyyyyyy

- + other yes
If you wish to restart the Duration Test press the 4th key and the test will continue.

Where XX is the displayed program number and yyyyyyyy the program name.

3-36 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

Function Test

3. Firmware and Software Description

This option is used to check various instrument component functions.

Service
other yes

Function Test
yes exit no

Motor Test
yes exit no

Solenoid Test
yes exit no

Sensor Test
yes exit no

Test Fill Verif.
yes exit no

Function Testing procedure

Select Options
yes exit no

Motor testing procedure

Solenoid testing procedure

Sensor testing procedure

Fill Verification testing procedure

Barcode Test
yes exit no

Orbit.Shake:
yes exit no

Liq.Sens.
yes exit no

uC: 80517A
exit yes

or

When the following message is displayed:
Function Test

yes exit no

Barcode Reader testing procedure

Orbital Shaking testing procedure
(This option is not used.)

Liquid Detection testing procedure

uC: 80537
exit yes

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-37

3. Firmware and Software Description

Press yes to enter the Function Test procedure and the following message is
displayed:

Motor Test
yes exit no

Use no to select the Function Testing procedure that is to be performed.
The following function test can be performed:

Motor Test Test the various motors
Solenoid Test Test the various solenoid valves
Sensor Test Test the various sensors.
Test Fill Verif. Test Fill Verify option
Barcode Test Test Barcode Reader
Liq. Sens. Test the Liquid Sensor System

Motor Testing

This option is used to check that the various motors are functioning correctly.

Function Test
yes exit no

Motor Test
yes exit no

MotTest:Plate
yes exit no

MotTest:Manifold
yes exit no

MotTest:Pumps
yes exit no

Motor testing procedure

Solenoid Test
yes exit no

Plate motor testing procedure

Manifold motor testing procedure

Pump testing procedure

To enter the Motor Testing procedure, press yes when the following message is
displayed:

Motor Test
yes exit no

The following message is displayed:
MotTest:Plate

yes exit no
Use no to select which type of motor is to be tested.

Plate Motor Plate support movement (forwards and backwards)
Manifold Motor Manifold movement (up and down)
Pumps Aspirating, dispensing and aerosol pumps switched on or off

3-38 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

y

y

y

y

Plate Motor Testing

Motor Test

MotTest:Plate

Platemotor: X

- + exit home

MotTest:Plate

es exit no

es exit no

es exit no

Plate motor testing procedure

MotTest:Manifold

X = Status of home position switch

Press '-' to move the plate support

out of the instrument.

Press '+' to move the plate support

into the instrument.

Press 'home' to return the plate support

to the home position.

Press 'exit' to exit from plate motor

testing procedure

es exit no

To test the plate support motor, press no until the following message is
displayed:

MotTest:Plate
yes exit no

Press yes to enter the plate motor testing procedure and the following message
is displayed:

Platemotor: X

- + exit home
Where X is the displayed status of the home position switch.

st

Use the 1

and 2nd keys to move the plate support.

Pressing the - and + keys moves the plate support out of the instrument.

When the plate support has been moved away from the home posi tion, pressing
home returns the plate support to the home position.

When the plate support motor testing has been completed, press exit and the
following message is displayed:

MotTest:Plate
yes exit no

Press no to select the next motor to be tested or press exit to return to the Select
Testing option.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-39

3. Firmware and Software Description

y

y

y

y

Manifold Motor Testing

Motor Test

es exit no

MotTest:Manifold

Manifoldmotor: X

- + exit home

MotTest:Manifold

es exit no

es exit no

X = Status of home position switch

Press '-' to rotate the motor anticlockwise

Press'+' to rotate the motor clockwise

Press 'home' to return the manifold

to the home position.

Press 'exit' to exit from manifold motor

testing procedure

Plate motor testing procedure

MotTest:Pumps

es exit no

To test the manifold motor, press no until the following message is displayed:
MotTest:Manifold

yes exit no
Press yes to enter the manifold motor testing procedure.

The following message is displayed:
Manifoldmotor: X

- + exit home
Where X is the displayed status of the home position switch (1 = home position)

Use the 1

st

and 2nd keys to rotate the manifold motor.
Pressing - rotates the motor anticlockwise.
Pressing + rotates the motor clockwise.
When the manifold has been moved away from the home position, pressing

home returns the manifold to the home position.
When the manifold motor testing has been completed, press exit and the

following message is displayed:
MotTest:Manifold

yes exit no
Press no to select the next motor to be tested or press exit to return to the Select

Testing option.

3-40 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

y

y

Asp

y

p

y

Pump Testing

Motor Test

MotTest:Pumps

0 XXX 0

Dis exit Aer

MotTest:Pum

es exit no

es exit no

s

es exit no

Plate motor testing procedure

MotTest:Plate

Press Asp to switch the aspirating pump

on (1) and off (0)

Press

Dis

on and off (when pump is rotating)

XXX = number of rotations performed)

Press Aer to switch the aerosol pump

on (1) and off (0)

Press exit to exit from the Pump Testing procedure

es exit no

to switche the dispensing pump

To test the pump motors, press no until the following message is displayed:
MotTest:Pumps

yes exit no
Press yes to enter the Pump Motor Testing procedure.

The following message is displayed:
0 XXX 0

Asp Dis exit Aer
Pressing Asp starts and stops the aspirating pump.

Pressing Dis starts and stops the dispensing pump.
Pressing Aer starts and stops the aerosol pump (if fitted).

Aspirating Pump

To test the Aspirating pump, press Asp to start the pump.
When the pump is started, the pump status is changed from 0 to 1.

1 XXX 0
Asp Dis exit Aer

Check that the aspirating pump is running.
If the pump is not running, the possible causes are:

Pump is defective Replace Pump
No power to the pump Replace Main Board, Power Supply Board

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-41

3. Firmware and Software Description

Aerosol Pump*

To test the Aerosol pump, press Aer to start the pump.
When the pump is started, the pump status is changed from 0 to 1.

0 XXX 1
Asp Dis exit

Check that the Aerosol pump is running.
If the pump is not running, the possible causes are:

Pump is defective Replace Pump
No power to the pump Replace Main Board, Power Supply Board

Dispensing Pump

To test the Dispensing pump, press Dis to start the pump.
When the pump is started, the number of rotations performed (XXX) is displayed.

0 XXX 1
Asp Dis exit

In firmware versions with the prime valve, the Aerosol pump doesn't function.
If the pump is not running or there is no current flow to the pump, the following

message is displayed:
0 0 0

Disp.Sens.Err.!!
The instrument is now locked with this error message, switch the instrument off

and on again.
If the pump is not running, the possible causes are:

Pump is defective Replace Pump
No power to the pump Replace Main Board, Power Supply Board

When the pump motor testing has been completed, switch all the pumps off and
press the exit.

The following message is displayed:
MotTest:Pumps

yes exit no
Press no to select the next motor to be tested or press exit to return to the Select

Testing option.

* In firmware versions with the prime valve, the aerosol pump does not function.

3-42 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

Solenoid Testing

This option is used to check that the various solenoid valves are functioning
correctly.

Function Test
yes no exit

Solnoid Test
yes exit no

Disp. Valve: Off
yes exit no

Valve Ch 1: Off

yes exit no

Valve Ch 2: Off
yes exit no

Valve Ch 3: Off
yes exit no

Solenoid testing procedure

Sensor Test
yes exit no

Switch dispensing solenoid
valve on and off

Switch channel 1 solenoid
valve on and off

Switch channel 2 solenoid
valve on and off

Switch channel 3 solenoid
valve on and off

Valve Ch 4: Off
yes exit no

Switch channel 4 solenoid
valve on and off

Prime Valve: Off
yes exit no

Switch prime solenoid valve on
and off

This valve is no longer fitted to the instruments

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-43

3. Firmware and Software Description

Pinch Valve

Channel 1

Solenoid Valve

Channel 2

Inlets for channels 1 - 4

Channel 3 Channel 4

The number of solenoid valves that is fitted is dependent on the number of
dispensing channels fitted.

* Some of the instruments may include the prime valve.

3-44 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

To select the Solenoid Testing procedure, press no until the following message is
displayed:

Solenoid Test
yes exit no

Press yes to enter the testing procedure and the following message is displayed:
Disp.Valve: OFF

yes exit no
Press yes to switch the dispensing solenoid valve on and off.

Check that the solenoid valve is activated (pinch valve in) when valve is switched
on.

Press no to select the next solenoid valve and the following message is
displayed:

Valve Ch. 1: Off
yes exit no

Press yes to switch the channel 1 solenoid valve on and off.
Press no to select the next solenoid valve and the following message is

displayed:
Valve Ch. 2: Off

yes exit no
Press yes to switch the channel 2 solenoid valve on and off.

Press no to select the next solenoid valve and the following message is
displayed:

Valve Ch. 3: Off
yes exit no

Press yes to switch the channel 3 solenoid valve on and off.
Press no to select the next solenoid valve and the following message is

displayed:
Valve Ch. 4: Off

yes exit no
Press yes to switch the channel 4 solenoid valve on and off.

Press no to select the next solenoid valve and the following message is
displayed:

Prime Valve:Off
yes exit no

Press yes to switch the channel prime valve on and off.
Press no to select the next valve and the following message is displayed:
When all the required valves have been tested, press exit and the following

message is displayed:
Solenoid Test

yes exit no
Press no to select the next testing procedure or press exit to return to the

Function Test option.

* Some of the instruments may include the prime valve.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-45

3. Firmware and Software Description

T

Sensor Function Test

This option is used to check that the various sensors are functioning correctly.

Function Test
yes no exit

Sensor Test
yes exit no

Sensor Test
XX XXX exit

To select the Sensor Testing procedure, press no until the following message is
displayed:

Sensor Test
yes exit no

Press yes and the instrument disables all the motors and the following message
is displayed:

Sensor Test
MM AXP exit

Where MM AXP WF is the displayed status of the various sensors

MM

Manifold type sensors (00 = no manifold, 01 = 8 way,

Solenoid testing procedure

est Fill Verif.

yes exit no

10 = 16 way, 11 = 12 way)

A
X
P

Move the various parts to check that the sensor are working correctly.
When the sensor testing has been completed, press exit and the following

message is displayed:
Sensor Test

yes exit no
Press no to select the next testing procedure or press exit to return to the

Function Test option.
When the sensor testing has been completed, press exit and the following

message is displayed:
Sensor Test

yes exit no
Press no to select the next Testing procedure or press exit to return to the

Function Test option.

Manifold arm home position sensor (1 = home position)
Plate mismatch sensor (1 = plate mismatch)
Plate support home position sensor (O = home position)

3-46 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

Fill Verification Testing

If the Fill Verify manifold is fitted, it can be tested using the following procedure.
To select the Fill Verify testing procedure, press no until the following message is

displayed:
Test Fill Verif.

yes exit no
Press yes to enter the testing procedure and the following message is displayed:
XX: yyyyyyyyyyyy

Asp Dis exit CLR
Where XX: is the type of manifold fitted (8,12 or 16) and yyyyyyyyyyyyy is the

status of the needles.

st

Use the 1
Pressing Asp starts and stops the aspirating pump.
Pressing Dis starts and stops the dispensing pump.
When the instrument is dispensing liquid the status of the needles changes.

and 2nd keys to start and stop the aspirating and dispensing pumps.

Press the CLR key to clear the display and then redisplay the new status.
When the testing has been completed, press exit and the following message is

displayed:
Test Fill Verif.

Yes exit no
Press no to select the next testing procedure or press exit to return to the

Function Test option.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-47

3. Firmware and Software Description

Barcode Reader

If the Barcode Reader option is fitted, it can be tested using the following
procedure.

To select the Barcode Testing procedure, press no until the following message is
displayed:

Barcode Test
yes exit no

Press yes to enter the testing procedure and the following message is displayed:

exit
Move the barcode reader over a barcode strip, when the barcode strip has been

read the instrument beeps and displays the read barcode number. For example:
AE 2345

exit

This procedure can now be repeated for another barcode strip.

When the testing has been completed, press exit and the following message is
displayed:

Barcode Test
yes exit no

Press no to select the next testing procedure or press exit to return to the
Function Test option.

3-48 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

Liquid Detection

If the Liquid Detection Option is fitted, it can be tested using the following
procedure.

To select the Liquid Detection testing procedure, press no until the following
message is displayed:

Liq.Sens.
yes exit no

Press yes to enter the testing procedure and the following message is displayed:
Liq.Sen.1 XXX Y

- + exit yes
Where XXX is the displayed value and Y is the limit (0 = lower 1 = higher)

Use the - and + keys to select the required liquid sensor to be tested.
(1 - 4 & W*)

When the required sensor is displayed, gently press on the bottle and check that
the displayed value has increased. Carefully lift the bottle and check that the
value has decreased.

When the testing has been performed on the selected liquid sensor, use the - and
+ keys to select the next required sensor.

When the testing has been completed on all the sensors, press exit and the
following message is displayed:

Liq.Sens.
yes exit no

Press no to select the next testing procedure or press exit to return to the
Function Test option.

* 1 - 4 and W are the five sensors

1 Green
2 Gray
3 Blue (4 liquid channels)
4 Purple (4 liquid channels)

W Waste

Show Detected CPU

uC: 80C517A
exit yes

uC: 80537
exit yes

Shows which version of micro-controller that is used on the main board.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-49

3. Firmware and Software Description

Exiting the Function Test

When all the required function tests have been performed, the function test
procedure is exited by pressing exit.

Service
other yes

Function Test
yes exit no

Motor Test
yes exit no

Solenoid Test
yes exit no

Sensor Test
yes exit no

Test Fill Verif.
yes exit no

Barcode Test
yes exit no

Function Testing procedure

Select Options
yes exit no

Motor testing procedure

Solenoid testing procedure

Sensor testing procedure

Fill Verify testing procedure

Barcode Reader testing procedure

Orbit.Shake:
yes exit no

Orbital Shaking testing procedure
(This option is not used.)

Liq.Sens.
yes exit no

Liquid Detection testing procedure

uC: 80517A

exit yes

or

uC: 80537

exit yes

The following message is displayed:
Function Test

yes exit no
Press yes to re-enter the Function Test submenu.

Press exit to exit from the Service menu.
Press no to select the next option in the Service menu.

3-50 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

1 8

X

A

o

Select Options

This option is used to select which options that are fitted to the instrument.

Select Options

yes exit n

Select fitted instrument options

dj Dispens Pump

yes exit no

Code:

1 2 1 4

Waste Full: XX

- + other yes

Barcode: XXX

- + other yes

Printer: XXX

- + other yes

Fill.Verif.: XXX

- + other yes

Manif. Det.: XXX

- + other yes

Enter required code '121418' (for firmware V4.20 and higher)

(Enter ‘1248’ for firmware V4.01 up to but not including V4.20)

Use '-' and '+' key to select if Waste Full
is fitted.

Press 'yes' to confirm and save setting
Press 'other' to move to next option
without saving setting

Use '-' and '+' key to select if Barcode
is fitted.

Press 'yes' to confirm and save setting
Press 'other' to move to next option
without saving setting

Use '-' and '+' key to select if Printer is fitted.

Press 'yes' to confirm and save setting
Press 'other' to move to next option
without saving setting

Use '-' and '+' key to select if Fill
is fitted.

Press 'yes' to confirm and save setting
Press 'other' to move to next option
without saving setting

Use '-' and '+' key to select if Manifold
is fitted.

Press 'yes' to confirm and save setting
Press 'other' to move to next option
without saving setting

Aerosol Pump: XXX

- + other yes

Liq.Sens. XXX

- + other yes

Orbit.Shake: XXX

- + other yes

Drip Mode: XXX

- + other yes

Options END

other yes

Adj. Prime

yes exit no

Use '-' and '+' key to select if Aerosol
is fitted.

Press 'yes' to confirm and save setting
Press 'other' to move to next option
without saving setting

Use '-' and '+' key to select if Liquid
is fitted.

Press 'yes' to confirm and save setting
Press 'other' to move to next option
without saving setting

Use '-' and '+' key to select if Orbital
is fitted. This option is not used.

Press 'yes' to confirm and save setting
Press 'other' to move to next option
without saving setting

Use '-' and '+' key to select if Drip Mode is used.

Press 'yes' to confirm settings
Press 'other' to move to next option without saving setting

Press 'yes' to exit procedure
Press 'other' to repeat procedure

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-51

3. Firmware and Software Description

When the following message is displayed:
Select Options

yes exit no
Press yes to enter the Select Options submenu and the following message is

displayed:
Code:

1 2 4 8

The Select Options submenu can only be entered by entering the required
code: 1248

When the code has been correctly entered, the following message is displayed:
Waste Full: NO

- + other yes
Use the - and + keys to set if the Waste Full Sensor option is fitted and to be

used.
NO = option not fitted and not used
YES = option fitted and used
Pressing other moves to the next option without saving the setting.
When the required setting is displayed, press yes to save the setting and the

following message is displayed:
Barcode: NO

- + other yes
Use the - and + keys to set if the Barcode option is fitted and to be used.

NO = option not fitted and not used
YES = option fitted and used
Pressing other moves to the next option without saving the setting.
When the required setting is displayed, press yes to save the setting and the

following message is displayed:
Printer: NO

- + other yes
Use the - and + keys to set if the Printer option is fitted and to be used.

NO = option not fitted and not used
YES = option fitted and used
Pressing other moves to the next option without saving the setting.
When the required setting is displayed, press yes to save the setting and the

following message is displayed:
Fill Verif.: NO

- + other yes
Use the - and + keys to set if the Fill Verify option is fitted and to be used.

NO = option not fitted and not used
YES = option fitted and used
Pressing other moves to the next option without saving the setting.

3-52 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

When the required setting is displayed, press yes to save the setting and the
following message is displayed:

Manif Det.: NO

- + other yes
Use the - and + keys to set if the Manifold Detection option is fitted and used.

NO = option not fitted and not used
YES = option fitted and used
Pressing other moves to the next option without saving the setting.

When the required setting is displayed, press yes to save the setting and the
following message is displayed:

Aerosol Pump NO

- + other yes
Use the - and + keys to set if the Aerosol Pump option is fitted and to be used.

NO = option not fitted and not used
YES = option fitted and used
Pressing other moves to the next option without saving the setting.

When the required setting is displayed, press yes to save the setting and the
following message is displayed:

Liq.Sens. NO

- + other yes
Use the - and + keys to set if the Liquid Detection option is fitted and to be used.

NO = option not fitted and not used
YES = option fitted and used
Pressing other moves to the next option without saving the setting.

When the required setting is displayed, press yes to save the setting and the
following message is displayed:

Orbit.Shake: NO

- + other yes
This option is not used.

Pressing other moves to the next option without saving the setting.
Drip Mode: NO

- + other yes
This option is only available together with the cell wash manifold (M8, M12, M16).

Use the - and + keys to set if the Drip Mode option is fitted and to be used.
NO = option not fitted and not used
YES = option fitted and used

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-53

3. Firmware and Software Description

When selecting yes the dispensing pump must be calibrated for Drip Mode
speed. For more information, see next page - Adjust Dispensing Pump.

When the required setting is displayed, press yes to save the setting and the
following message is displayed:

Options END
other yes

Press other to return to the start of the select options submenu.
Press yes to exit from the Select Options submenu and the following message is

displayed:
Adj. Prime Pos.

yes exit no

3-54 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

A

Adjust Dispensing Pump

This option is used to calibrate the dispensing pump so that the correct volume of
solution is dispensed with each manifold that is used.

Service
other yes

Adj Dispens Pump

yes exit no

Disp. Speed X

- + exit yes

Weight_300: xx.x

- + exit yes

Weight_100: xx.x

- + exit yes

Adj Dispens Pump
yes exit no

djust Dispensing Pump option

Service
other yes

Select required dispensing speed
(3 or drip)

Enter weight for 300 µl

Enter weight for 100 µl

Repeat procedure for another
dispensing speed

The delivered instrument has been calibrated for the supplied manifolds.
Calibration must be performed if another manifold is to be used or if the fitted
manifold is exchanged for another manifold of the same type.

Before the calibration procedure can be performed, programs that dispense
300 µl and 100 µl solution into each well must be defined with the required
dispensing speed.

The dispensing pump is calibrated using the following procedure:
Weight 300

1. Accurately weigh a clean dry microplate (x)

2. Select the program that dispenses 300 µl of solution into each well.

3. Accurately weigh the microplate containing the solution. (y)

4. Calculate the weight of solution (y - x = Weight 300)

Weight 100

1. Accurately weigh a clean dry microplate (x)

2. Select the program that dispenses 100 µl of solution in to each well.

3. Accurately weigh the microplate containing the solution. (z)

4. Calculate the weight of solution (z - x = Weight 100)

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-55

3. Firmware and Software Description

Enter the Service menu and scroll through the options until the following message
is displayed:

Adj Dispens Pump
yes exit no

Press yes and the following message is displayed:
Disp. Speed X

- + exit yes
Use the - and + keys to select the required dispensing speed.(3 or drip)

When the required dispensing speed is displayed, press yes and the following
message is displayed:

Weight_300: xx.x

- + exit yes
Where XX.X is the displayed weight of 300 µl solution in each well.

If the displayed weight is not correct use the 1
weight.

Pressing - decreases the weight by 0.1 g.

st

and 2nd keys to scroll the correct

Pressing + increases the weight by 0.1 g.
When the required weight is displayed, press yes and the following message is

displayed:
Weight_100: xx.x

- + exit yes
Where XX.X is the displayed weight of 100 µl solution in each well.

If the displayed weight is not correct, use the 1

st

and 2nd keys to scroll the correct

weight.
Pressing - decreases the weight by 0.1 g.
Pressing + increases the weight by 0.1 g.
When the required weight is displayed, press yes and the following message is

displayed:
Adj Dispens Pump

yes exit no
When the calibration has been performed press exit to exit from the Service

menu or press no to select the next option in the Service menu.

Repeat the procedure with Disp. Rate: Drip if this option is enabled.

Ensure that the calibration procedure is repeated if the weight (300 µl) is

greater than 33 g or less than 24 g before calibrating the instrument for the

first time.

3-56 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

A

Adj. Prime Pos.

This option is used to adjust the position of the plate support for the Prime/Rinse
procedures.

The defined position for 8 and 12 way manifolds is different to that of the 16 way
manifold. It may be necessary to adjust the position of the 16 way manifold
otherwise the solution could be dispensed outside the waste sink.

Use the following procedure to adjust the Prime Position.

Service
other yes

Adj Prime Pos.

yes exit no

Prime Pos.: XXX

- + exit yes

djust Prime Position option

Service End
yes no

Use the '-' and '+' keys to position
the waste sink correctly under
the manifold

Service End
yes no

When the following message is displayed:
Adj.Prime Pos.

yes exit no
Press yes to enter the Prime Position following message is displayed:
Prime Pos.: 75

- + exit yes
Use the - and + keys to move the position of the plate support.

Pressing - moves the plate support more under the manifold.
Pressing + moves the plate support out from under the manifold.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-57

3. Firmware and Software Description

Waste Sink

Plate Support

When a key is pressed, the instrument first moves the plate support to the home
position and then moves the plate support to the new prime / rinse position.

Each press of the key moves the plate support by five steps.
When the plate support is correctly positioned so that no solution is dispensed

outside the waste sink, press yes, the position is saved and the following
message is displayed:

Service End
yes no

3-58 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

Adj. Plate Ref.

A

A

3. Firmware and Software Description

The Adjust Plate Reference is used to define the plate position in order that the
manifold needles are correctly positioned. The adjustment should always be
positioned as shown below for all manifolds. The procedure is the same for
manifolds; 8, 12 and 16. Place the adjustment plate on the plate support and
enter the Service menu.

alignment

points for the

8 manifold

needle

djustment Plate

Use the - and + keys to move the manifold needles in the X (left and right) and Z
(up and down) directions. The needles must be set exactly on the crosses
marked on the adjustment plate.

Service
other yes

Adj Plate Ref.

yes exit no

Ref. Pos. X: 390

- + exit yes

djust Plate Reference option

Service
other yes

Position the manifold needles
using the '-' and '+' keys to move
left and right

Ref.Pos. Z: 130

- + exit yes

Position the manifold needles
using the '-' and '+' keys to move
up and down

Ref. Pos. OK?
yes no

Init Manifold

Confirm that the set
positions are correct

Manifold is initialized

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-59

3. Firmware and Software Description

Service End

This option is used to select if the Service menu is to be exited.

Service End
yes no

Run X:yyyyyyyy

- + other yes

When the following message is displayed:
Service End

yes no
Select if the Service menu is to be exited.

Remain in Service Menu

Press no to remain in the Service menu and the following message is displayed:
Clear All ?

yes exit no
Select the required option in the Service menu.

Exit Service Menu

Exit Service Menu

Clear All ?
yes exit no

Standby Mode

Press yes and the following message is displayed:
Run X:yyyyyyy

- + other yes
The instrument is now in the Standby mode and can be used to wash a

microplate.

3-60 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

3.3 External Software

3.3.1 Columbus Computer Software

Consisting of WinWash and Download including the operating manual an d
RS232 interface cable.

3.3.2 Firmware Update

Consisting of the Download software, the latest version of the firmware 3.x, as
well as 5 language software files (English, German, French, Spanish and Italian)
that can change the on-board language of the Columbus (from firmware level

2.23 onwards) in the field.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-61

3. Firmware and Software Description

3.4 Serial Interface

A description of the serial interface fitted to the instrument.

3.4.1 Hardware Specifications

The instrument is controlled by the computer and the communication between the
computer and the instrument is done through a RS-232-C interface.

The interface specification may be either to the CCITT interface standard or to
the EIA RS-232-C interface standard.

Pin Designation

The illustration below shows the pin assignment of the DB 9 connector fitted to
the instrument.

Pin 5

Pin 9

All connected devices must be approved and listed as per EN 60950, UL 195 0 or
CSA C22.2 No. 950 for Data Processing Devices

Pin 1

Pin 6

3-62 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

RS-232-C Interface Lines

The Serial Interface of the instrument is connected to a start-stop synchronized
serial RS-232-C circuit.

The list below names the interface lines of the instrument's 9 pin connector.

PIN Number DESCRIPTION

1 RS 485 IN/OUT (Option)
2 (RS232) TD Transmit Data
3 (RS232) RD Receive Data
4 RS 485 IN/OUT
5 GND Ground
6 RS-config: if connected to GND RS 485, if open RS 232

is active (only if jumper x100 on the mainboard is open!)
7 No connection
8 RS232 CTS Clear To Send
9 +24V INPUT (Fused 4AF)

The connecting cable used to connect the instrument to the computer should b e
wired as given below:

INSTRUMENT COMPUTER

TD connected to RD
RD connected to TD

GND connected to GND
RTS connected to CTS
DSR connected to DCD and DTR

Use the computer handbook to find the correct pin connections.

Instrument InstrumentComputer 25 pin Computer 9 pin

1
2
3
4
5
6
7
8
9

20

1
2
3
4
5
6
7
8

1
2
3
4
5
6
7
8
9

1
2
3
4
5
6
7
8
9

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-63

3. Firmware and Software Description

Synchronization and Data Format

For the instrument to communicate with the computer correctly, the instrument
and the computer must be set for the same communication parameters.

Check that following parameters are set correctly on both the comp uter and the
instrument:

1. Start bit length:

2. Stop bit length:

3. Data format:

4. Parity bit:

5. Baud Rate:

6. Mode:

Daisy Chaining Instruments

The instrument can be connected to a computer, which controls a number of
instruments using a

Computer

A ddress #30

1 Bit
1 Bit
8 Bits
None
9600 Baud
half duplex

Daisy Chain cable. (RS 485 version only).

Dai sy Chain Cable

Washer 1

A ddress #31

Washer 2

A ddress #32

Washer 3

A ddress #33

Each of the instruments must be defined with a different device address

Each instrument can be controlled by sending the commands to the req uired
device address.

To select a different device address, start the Remote Mode with the default
device address (#31) and send the command to X 37 (to sele ct the device
address #37).

3-64 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

3.4.2 Contents

A. Protocol 3 – 65
B. Commands - Overview 3 – 67
C. Commands - Description 3 – 69

1. Switch communication on/off 3 – 69

2. Starting and Stopping a Program / Prime
Procedure /Rinse Procedure

3. Define Programs 3 – 70

Define Header / Procedure 3 – 70
Define Program Lock Status 3 – 70
Common parameters 3 – 71
Procedures 3 – 71

4. Upload Program 3 – 73

Upload Header 3 – 73
Upload Procedure 3 – 73
Read Program Lock Status 3 – 73

5. Clear Program 3 – 74

6. Define Plates 3 – 74

Write Plate name / Define Plate Lock Status 3 – 74

7. Upload Plate Data 3 – 75

Read Plate name / Plate Lock Status 3 – 75

8. Define Rinse Parameters 3 – 75

3 – 69

9. Upload Rinse Parameters
Read Prime Time

10. Read Device Information 3 – 76

11. Read Header & Firmware Version 3 – 76

12. Define Power on Header 3 – 76

13. Read Option Status 1 & 2 3 – 77

14. Define Option Status 1 & 2 3 – 78

15. Read Device Status 3 – 78

16. Read Number of Liquid Channels 3 – 79

17. Read Manifold Information 3 – 79

18. Define Manifold Type 3 – 79

19. Read Dispense Rates 3 – 79

3 – 76

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-65

3. Firmware and Software Description

20. Define Dispense Rates 3 – 80

21. Read Aspirate Rates 3 – 80

22. Define Aspirate Rates 3 – 80

23. Calibrate Dispense Pump 3 – 81

24. Read Total Test Time & Power On Time 3 – 81

25. Clear Total Test Time & Power On Time 3 – 81

26. Adjust Times 3 – 82

27. Change Device Address 3 – 82

28. Read Plate Identity (Barcode) 3 – 82

29. Clear All 3 – 82

30. Start Flash Download Mode 3 – 82

31. Read / Write Settings 3 – 83

32. Write time & date 3 – 83

33. Enable / Disable Fill Verification 3 – 83

34. Read Fill Verification Needle Status 3 – 84

D. Examples for remote control - command sequences 3 - 85

Enter Remote Mode 3 – 85
Leave Remote Mode 3 – 85
Change Device address 3 – 85
Start Program 3 – 86
Start Prime 3 – 86
Download Program 3 – 87
Download Plate 3 – 90
Download Rinse Parameter 3 – 91

3-66 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

3.4.3 A. Protocol

1. Data format

Baud Rate: 9600 Baud
Databits: 8
Parity: No
Stop Bit: 1
Mode: Half duplex

Command Block Format (Master -> COLUMBUS)

hex dec keyboard
0

1 STX 0x02 2 ALT-'2' / ^B
2

3 Sequence Number 0x31 49 '1'
4

4 + n ETX 0x03 3 ALT-'3' / ^C
5 + n Checksum

Synchronization
character

Device Address
(Columbus Add.)

Datablock (n
characters long)

0xFF 255 ALT-'255'

0x32 50 '2' (example)

Answer Block Format (COLUMBUS -Master)

0 Synchronization character (FFh) 0xFF 255 ALT-'255'
1 STX (^B or 02h) 0x02 2 ALT-'2' / ^B
2 Master Address (30h) 0x30 48 '0'
3 Status Code
4 Datablock (n characters long)
4 + n ETX (^C or 03h) 0x03 3 ALT-'3' / ^C
5 + n Checksum
6 + n Line turn around character 0xFF 255 ALT-'255'

a.) Synchronization character for Bus Synchronization
b.) STX (^B or 02h) start of Command string
c.) Device Address is a hexadecimal number for every device on the bus

(for addressing in 'Daisy Chain')

d.) Master Address is the address of the host system. This address is always 30hex.
e.) Status includes device status (Ready, Busy) and the error codes.
f.) Sequence Number (for repetitions of commands at incorrect transmission).
g.) Datablock includes data and/or commands received from or sent to the washer.
h.) ETX (^C or 03h) defines the end of the command/data-string (except CS character).
i.)

j.) Line turn around character - to make sure that the last byte (Checksum) is received

Checksum is the last byte of the command/data-string. All bytes (except
synchronization and checksum) are XOR-calculated. The receiver compares the sent
checksum with the calculation. When they are different the command/data-string is
ignored (Error).

complete. It is possible that this character will not be sent complete.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-67

3. Firmware and Software Description

3.4.4 Sequence Number

7 6 5 4 3 2 1 0
0 0 1 1 REP SQ2 SQ1 SQ0

SQ0 ... SQ2 1 ... 7 Sequence Number, 0 not allowed
REP 1 Repeated Command
0 First Command

Status Code

7 6 5 4 3 2 1 0
0 1 RDY 0 EC3 EC2 EC1 EC0

EC0 ... EC4 0 No Error
1 Init Error
2 Invalid Command
3 Invalid Operand
4 Invalid Command Sequence
5 System Error (not used)
6 res.
7 Plate Mismatch
8 Plate Transport Error
9 Manifold Transport Error
10 Dispense Pump Error
11 Fill Verification Error
12 Manifold Error
13 Waste Full Error
14 Test Not Defined
15 Command Overflow
16 Fill Verification Function Error
17 Fill Verification Aspiration Error

RDY 1 Ready
0 Busy

3-68 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

3.4.5 B. Commands - Overview

Command Abr. Parameter 1 Parameter 2 Page

Read Dispense Rates A1 08/12/16 3 – 79
Define Dispense Rates A2 08/12/16 Drip 005 - 050

6x 000 - 999
Read Aspirate Rates A3 3 – 80
Define Aspirate Speed A4 5x 000 - 999 3 – 80
Read Total_ Test_Time &

Power_On_Time
Clear Total_ Test_Time &

Power_On_Time
Set_Time (Sort_of_Time, Time) AT p / r / a 00 - 99 (24) 3 – 82
Download_Program (Prog. Nr., ...) D 01 - 30 3 – 70
Clear Program (Prog. Nr) DC 01 - 30 3 – 74
Download Header/Procedure

(Select, ...)
Remote_Mode_OFF E 3 – 69
Start_Flash_Load_Mode FL 3 – 82
Read Fill Verification Needle Status FR 3 – 84
Enable / Disable Fill Verification FV 0 / 1 3 – 83
Start_Prime (Liquid Channel) GP 1 - 4 3 – 69
Start_Rinse (Modus, Liquid Channel) GR 0 - 3 0 - 4 3 – 69

A6 3 – 81

A7 3 – 81

DL 00 - 58 3 – 70

3 – 80

Stop Program / Prime procedure HP 3 – 69
Stop_Rinse HR 3 – 69
Read_Device_Status I 3 – 76
Define Options 2 LA '0' , '1' 3 – 78
Define Power On Header LH 'Header' 3 – 76
Select Options LO '0' , '1' 3 – 78
Read_Manifold_Information M 3 – 79
Define Manifold Type MA 08 / 12 / 16 3 – 79
Define_Program_Lockstatus N ´#´,´ ´ 3 - 70

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-69

3. Firmware and Software Description

Read_Program_Lockstatus NL 3 – 73
Read Options_1 O 3 – 77
Read Options_2 OA 3 – 77
Read Plate ID (Barcode) PI 3 – 82
Download_Plate (Plate Nr., ...) PD 1-9 ... 3 – 74
Read_Platename (Plate Nr.) PR 1-9 3 – 75
Upload_Plate (Plate Nr.) PU 1-9 3 – 75
Write_Platename (Plate Nr., ...) PW 1-9 ´Plate name´ 3 – 74
Query Q 3 – 78
Download_Rinse_Para. (Rtime,ARtime) RD 01 - 99 01 - 24 3 – 75
Upload_Prime_Parameter RP 3 – 76
Upload_Rinse_Parameter RU 3 – 76
Read Setting RS 0 - 8 0 - 4 3 – 83
Start_Program (Prog. Nr., Nr. of Strips) S 01 - 30 01 - 12 (8, 6) 3 – 69
Start_Program (Prog. Nr., Sel. Strips) ST 01 - 30 12 / 8 x 0 / 1 3 – 69
Upload_Program(Prog.Nr.) U 01 - 30 3 – 73
Upload Header/Procedure (Select, ...) UL 00 - 58 3 – 73
Calibrate Dispense Pump (Speed 1 – 5) W 00.0 –

Calibrate Dispense Pump (Speed Drip) WD 00.0 –

Write Settings WS 0 - 8 3 – 83
Write Time & date WT ... 3 – 83
Read_Number_Of_Solenoids V 3 – 79
Change_Device_Address (New Address) X 0x31 -

Clear_All XX 3 – 82
Remote_Mode_ON Y / ! 0 / 1 / 'M' 3 – 69
Read_Header & Firmware Version Z / ? 3 – 76

For Genalyser – Firmware Version 3.06

Command Abr. Parameter Validity Page

Abort Program FA None Run time 3 – 84

3 – 81

99.9
3 – 81

99.9

3 – 82

0x3F

Continue Program FC None Run time 3 – 85
Last Processed Strip FS None Run time 3 – 85
Fill Verification Status F? None Always 3 – 85

3-70 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

3. Firmware and Software Description

3.4.6 Commands - Description

1.Switch Communication On/Off

Command Parameter Answer
Remote ON ! or Y <p> p = 0: send no end after

program & prime
p='M': MINILYSER Mode
(no 'BUSY' in 'ACK'
else: send end.

Remote OFF E - ACK

+CW

2. Starting and Stopping a Program / Prime Procedure /Rinse Procedure

Command Parameter Answer
Start Program 1 S<p1><p2> p1: number of program

p2: number of strips

Start Program 2 ST<p1><p2> p1: number of program

p2: 8(12) x 0/1 selection of

strips
Start Prime GP<p> p: liquid channel ACK
Start Rinse GR<p1><p2> p1: Rinse mode

0:SOAK 1: DAY

2: NIGHT 3: AUTO

ACK

ACK

ACK

p2: liquid channel

(0 = all channels)
Stop Rinse HR - ACK
Stop Program/Prime HP - ACK

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3. Firmware and Software Description

3. Define Programs

Command Parameter Answer

Define Header

Define Procedure

Download_Program D<p1><p2>

<p3><p4>...

Command Parameter Answer
Download_header DL<p1><p2>

<p3><p4>...

Command Parameter Answer
Download_procedure DL<p1><p2>

p1: Program number
p2: Number of following

columns
p3: common parameters
p4: parameters of

procedures

p1: Selection 00: Header
p2: Program number
p3: number of following

columns
p4: common parameters

p1: Procedure number 01 - 59

ACK

ACK

ACK

Define Program Lock Status

Command Parameter Answer
Define Program Lock Status N<p1>

<p3><p4>...

<p2>

p2: Program number

p3: parameter of procedures

p1: Program number
p2: Lock Status
P2 = ´#´

P2 = ´ ´

⇒ Prog. locked

⇒ Prog. unlocked

ACK

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3. Firmware and Software Description

Common Parameters

Program name:
Plate type: 1 - 9 9
Manifold type: 08 / 12 / 16 10
Dispense rate: 0 - 5 /0=drip 12
Aspirate rate: 1 - 5 13
Aspirate mode: 0 - 3 14

Global / Final Asp.

0: normal / normal
1: crosswise /

crosswise
2: normal /

crosswise
3: crosswise /

normal
Working mode: 0 / 1 plate / strip mode 15
Strip selection: 0 / 1 NO / YES 16
Printer mode: 0 / 1 / 2 NO / YES / ASK 17
Final Asp. Time: 00 - 20 (in sec)/ 00 = NO 18

Final Asp. Speed: 00 - 20 (in mm/s) 20

st

1

        '

'

& 8th character (8 characters)

th

character (1 character)

th

& 11th character (2 characters)

th

character (1 character)

th

character (1 character)

th

character (1 character)

th

character (1 character)

th

character (1 character)

th

character (1 character)

th

& 19th

(2 characters)

character

th

& 21th

(2 characters)

character

Procedures

1. Wash

2. Aspirate

Code: 'W'

1. Parameter: Aspirate Speed 01-20 2

2. Parameter: Dispense / Aspirate Position 0/1 4

1 character

nd

& 3rd character

th

character
Parameter Dispen se Po stion Aspirate position
0 Bottom Bottom aspirate
1 Overflow Bottom aspirate
2 Bottom Bottom wash
3 Overflow Bottom wash

th

3. Parameter: Dispense Channel 1-4 5

4. Parameter: Dispense Volume 0050-3000 6

character

th

– 9th character (in µl)

Code: 'A' 1th character

th

1. Parameter: Aspirate Time 01-20 2

2. Parameter: Aspirate Speed 01-20 4

& 3th character

th

& 5th character

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3. Firmware and Software Description

3. Dispense

4. Soak

5. End a. End of Cycle

Code: 'D' 1th character

th

1. Parameter: Dispense Position 0/1 2

2. Parameter: Dispense Channel 1-4 3

3. Parameter: Dispense Volume 0050-0400 4

character

th

character

th

& 7th character (in µl)

Code: 'S' 1 character

nd

1. Parameter: Soak Time 001-999 2

2. Parameter: Shake Speed 0-4 5

- 4th character in sec

th

character
0 No shaking
1-3 Linear shaking
4 Orbital shaking

th

3. Parameter: Shake Mode 0-2 6
Parameter: Shake Speed (orbital) 0-2 6

character

th

character

Code: 'E' 1st character

nd

1. Parameter: Type of END 0 2

2. Parameter: Cycle Number 00-30 3

3. Parameter: Repeat Number 00-09 5

4. Parameter: Proc. in Cyc. 01-59 7

character

rd

& 4th character

th

& 6th character

rd

& 8th character

b. End of Program

Code: 'E' 1st character

1. Parameter: Type of END 1 2

nd

character

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3. Firmware and Software Description

4. Upload Program

Command Parameter Answer

Upload Header

Upload Procedure

Upload_Program U<p1> p1: Program

number

Command Parameter Answer
Upload_header UL<p1><p2>

<p3><p4>

Command Parameter Answer
Upload_procedure UL<p1><p2>

<p3>

p1: Selection
00: Header

p2: Program
number

p1: Procedure
number

p2: number of following
columns

p3: common parameters
p4: parameters of

procedures
(see 3. Define Programs)

p3: number of
following columns

p4: common
parameters

p3: parameters
of procedures

Read Program Lock Status

Command Parameter Answer
Read Program Lock Status NL p1: Program

p2: Program
number

number

p2: Lock Status
p2 = ´#´

Locked
P2 = ´ ´

unlocked

⇒ Prog.

⇒ Prog.

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-75

3. Firmware and Software Description

5. Clear Program

Command Parameter Answer

6. Define Plates

Clear_Program DC<p1> p1: Program

number

Command Parameter Answer
Download_Plate PD<p1><p2>

<p3><p4>...

Plate-Nr .: 1 - 9
Bottom form 0 / 1 (0 -> flat, 1 -> round)
Bottom aspirate position (4 characters)
Bottom wash position
Overflow position
Dispense position
Aspirate position 1
Aspirate position 2

p1: Plate Number
p2: Bottom Form
p3: Bottom Aspirate

Position
p4: Bottom Wash

Position
p5: Overflow Position
p6: Dispense Position
p7: Aspirate Position 1
p8: Aspirate Position 2

( -

║- )
║- )

( -

║- )

( -

║- )

( -

║- )

( -

ACK

ACK

Write Plate name / Define Plate Lock Status

Command Parameter Answer
Write_Platename PW<p1><

p2>

Plate-Nr .: 1 - 9
Platename (9 characters): 1 - 8: Name
9: Lock status of

3-76 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02

p1: Plate Number
p2: ´PLATENAME´

plate

ACK

´#´

⇒ Plate locked

´ ´

⇒ Plate unlocked

3. Firmware and Software Description

7. Upload Plate Data

Command Parameter Answer
Upload_Plate PU<p1> p1: Plate Number <p2><p3><p4>...

• p2: Bottom Form

• p3: Bottom Aspirate Position

• p4: Bottom Wash Position

• p5: Overflow Position

• p6: Dispense Position

• p7: Aspirate Position 1

• p8: Aspirate Position 2

Read Plate name / Plate Lock Status

Command Parameter Answer
Read_Platename PR<p1><p2> p1: Plate Number p2: ´PLATENAME´

Plate-Nr .: 1 - 9
Platename (9 characters): 1 - 8: Name
9: Lock status of plate

8. Define Rinse Parameters

Command Parameter Answer
Download_Rinse_

Parameter

p1: Rinse Time (01 - 99 / in seconds)
p2: Auto Rinse Time (01 - 2 4 / in hours)

RD<p1><p2> p1: Rinse Time

p2: Auto Rinse
Time

´#´

⇒ Plate locked

´ ´

⇒ Plate unlocked

ACK

2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3-77

3. Firmware and Software Description

9. Upload Rinse Parameters

Command Parameter Answer
Upload_Rinse_Parameter RU - <p1><p2>

p1: Rinse Time (01 - 99 / in seconds)
p2: Auto Rinse Time (01 - 24 / in hours)

Command Parameter Answer
Read_Prime_Time RP - <p1>

p1: Prime Time (01 - 99 / in seconds)

10. Read Device Information

Command Parameter Answer
Read_Device_Information I - <p1>(<p2><p3>)

p1: Sort of Procedure (P, R, T, S)
P: Prime active
R: Rinse active
T: Testrun (Program) active
S: Standby
p1 = 'T':
p2: Number of Program
p3: Number of Strip in Progress

11. Read Header & Firmware Version

Command Parameter Answer
Read_Model&FW-Version Z ? - <p1>

p1: String of 32 characters

• 0 ... 15: Power On Header

• 16 ... 31: Firmware Version

12. Define Power On Header

Command Parameter Answer
Define_Header LH <p1> ACK

p1: String of 16 characters (Power On Header)

3-78 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02