Cavro® XLP 6000 Modular Syringe Pump Operating Manual, 734237-C, en
Operating Manual
Cavro
®
XLP 6000 Modular Syringe Pump
Tecan Systems, Inc.
2450 Zanker Road
San Jose, CA 95131 USA
T 1 408 953 3100, Toll Free 1 800 231 0711
F 1 408 953 3101
E-mail: tecansystemsinfo@tecan.com
Web Site: www.tecansystems.com
Cavro® XLP 6000 Modular Syringe Pump Operating Manual, 734237-C, en
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Product Warranty Information
Tecan Systems warrants that instruments manufactured and sold by Tecan
Systems will be free from defects in materials and workmanship for a period of
twelve (12) months from the date of shipment to customer. Tecan Systems’
liability for the breach of the foregoing warranty is limited to the repair or
replacement of the products found to be other than warranted. Such products will
be accepted for return only if the customer returns them to Tecan Systems’
factory or repair depot within thirty (30) days from the time of discovery of the
alleged defect, and prior to return, fills out a Certificate of Decontamination
(document P/N 730171), obtains a return authorization number from Tecan
Systems, provides Tecan Systems with the serial number of each instrument to
be returned, and prepays freight charges to the factory or a designated Tecan
Systems repair depot. No warranty is expressed or implied for:
BreakageSyringes
MaltreatmentSyringe seals
Unauthorized serviceTubing and tubing
connections
Units not returned in original or
Cavro valves
adequate packaging
Units which are “life-cycled”Cavro probes
The foregoing warranties and limitations are customer’s exclusive remedies and
are in lieu of all other warranties, express or implied, including without limitation
any warranty of merchantability or fitness for a particular purpose.
Product Documentation Warranty Information
The information contained in this document is subject to change without notice.
Tecan Systems makes no warranty of any kind with regard to this material,
including, but not limited to, the implied warranties of merchantability and fitness
for a particular purpose.
Tecan Systems shall not be liable for errors contained in this document or for
incidental or consequential damages in connection with the furnishing,
performance, or use of this material.
Cavro® XLP 6000 Modular Syringe Pump Operating Manual, 734237-C, en
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Cavro® XLP 6000 Modular Syringe Pump Operating Manual, 734237-C, en
1Getting Started
Congratulations on your purchase of the Cavro® XLP 6000 Modular Syringe
Pump from Tecan Systems.
The XLP 6000 is a fully programmable, open frame, precision liquid handling
pump module, designed for applications in the 5 µL to 25 mL range. It is controlled
by an external computer or microprocessor and automates pipetting, diluting, and
dispensing functions.
This chapter includes these topics:
Regulatory Considerations
XLP 6000 Features at-a-Glance
Unpacking the XLP 6000
Functional Description of the XLP 6000
Safety
Tips for Setting Up the XLP 6000
Regulatory Considerations
1 - Getting Started
1.1Regulatory Considerations
The XLP 6000 is a general laboratory module. Since it not a medical device, it is
not subject to FDA regulatory approval. The XLP 6000 uses only recognized
components and bears the UL Recognized Component Mark:
The use of UL Recognized components in a product or system allows UL to focus
the evaluation of the complete system on its intended end-use, and thus speeds
up the evaluation of that product or system. For more information regarding UL
certification of Tecan Systems’ syringe pumps, please visit the Underwriters
Laboratories Inc. website at http://www.ul.com/info/st andard.htm. Tecan Systems’
customer file number for the recognized component is E164638.
1.1.1CE
As a module designed for incorporation into larger systems that require
independent testing and certification, the XLP 6000 does not carry its own CE
mark.
The XLP 6000 can radiate radio frequency energy , which may cause interference
The XLP 6000 can radiate radio frequency energy , which may cause interference
to radio and television communications. Follow standard good engineering
to radio and television communications. Follow standard good engineering
practices relating to radio frequency interference when integrating the XLP 6000
practices relating to radio frequency interference when integrating the XLP 6000
into electronic laboratory systems.
into electronic laboratory systems.
1.2XLP 6000 Features at-a-Glance
1.1XLP 6000 Features at-a-Glance
The XLP 6000 is a syringe pump that is designed for OEM precision liquid
The XLP 6000 is a syringe pump that is designed for OEM precision liquid
handling applications. It has the following standard features and functions:
handling applications. It has the following standard features and functions:
Syringe sizes ranging from 50 L to 25 mL
Syringe sizes ranging from 50 L to 25 mL
Accuracy 1.0% at full stroke
Accuracy 1.0% at full stroke
Precision 0.05% at full stroke
Precision 0.05% at full stroke
Standard dispense/aspirate resolution of 6,000 increments
Standard dispense/aspirate resolution of 6,000 increments
Microstep dispense/aspirate resolution of 48,000 increments
Microstep dispense/aspirate resolution of 48,000 increments
3-Port, 4-Port, Y-Block, T-Valve, 3-Port Distribution, 6-Port Distribution, and 9-
3-Port, 4-Port, Y-Block, T-Valve, 3-Port Distribution, 6-Port Distribution, and 9-
Borosilicate glass, Kel-F and Teflon fluid contact
Borosilicate glass, Kel-F and Teflon fluid contact
RS-232, RS-485 and CAN interface
RS-232, RS-485 and CAN interface
Programmable plunger speeds from 1.2 sec/stroke to 160 min/stroke, with
Programmable plunger speeds from 1.2 sec/stroke to 160 min/stroke, with
Teflon coated lead screw drive with a quadrature encoder for lost-step
Teflon coated lead screw drive with a quadrature encoder for lost-step
Pump diagnostics, self-test, and error reporting
Pump diagnostics, self-test, and error reporting
Auxiliary inputs and outputs
Auxiliary inputs and outputs
Operates using a single 24V DC power supply
Operates using a single 24V DC power supply
Port Distribution valves
Port Distribution valves
ramps and on-the-fly speed changes
ramps and on-the-fly speed changes
detection
detection
1.3Unpacking the XLP 6000
1.2Unpacking the XLP 6000
To unpack the module, follow these steps:
To unpack the module, follow these steps:
1Remove the pump module(s) and accessories from the shipping cartons.
1Remove the pump module(s) and accessories from the shipping cartons.
2Check the contents against the packing slip to make sure that all the
2Check the contents against the packing slip to make sure that all the
components are present.
components are present.
1.3.1ESD Considerations
1.2.1ESD Considerations
The XLP 6000 is an electronic device that is sensitive to electrostatic discharge
The XLP 6000 is an electronic device that is sensitive to electrostatic discharge
(ESD). Static discharge from clothing or other fixtures can damage these
(ESD). Static discharge from clothing or other fixtures can damage these
components. To prevent premature failure of pump components, use good ESD
practices when handling the XLP 6000. These include, but are not limited to:
Using wrist or ankle straps
ESD mats or worktables
ESD wax on the floor
Prepare an ESD-free work area before the chassis is grounded.
1.4Functional Description of the XLP 6000
The XLP 6000 uses a stepper-motor driven syringe and valve design to aspirate
and dispense measured quantities of liquid. Both the syringe and the valve are
replaceable. Functional descriptions and illustrations of each major XLP 6000
component are provided in the following sections.
The syringe plunger is moved within the syringe barrel by a lead screw drive that
incorporates a 1.8 stepper motor and quadrature encoder to detect lost steps.
The syringe drive has a 60 mm travel length and resolution of 6,000 increments
(48,000 increments in fine-positioning and microstep mode). When power is not
applied to the pump, the syringe drive can be removed by releasing the pin
assembly.
The base of the syringe plunger is held to the drive by a knurled screw. The top of
the syringe barrel attaches to the pump valve by a 1/4-28" fitting.
Figure 1-2, ’Syringe Components” shows the components of a typical syringe.
Figure 1-2Syringe Components
Syringes are available in these sizes: 50 L, 100 L, 250 L, 500 L, 1.0 mL,
2.5 mL, 5.0 mL, 10 mL, and 25 mL.. For ordering information, see Appendix A,
"Ordering Information".
Note: To obtain optimal performance and maximum life from any syringe, please
follow the syringe cleaning and maintenance procedure included with each Cavro
Syringe. Technical Note #1 (PN 730317) contains practical tips for the use and
maintenance of Cavro Syringes.
1.4.2Valve and Valve Drive
The valve is made of a Kel-F body and Teflon plug. The plug rotates inside the
valve body to connect the syringe port to the various input and output ports. In
addition to input and output ports, non-distribution valves also have a bypass
position. This position “bypasses” the syringe and connects the input and output
ports. The bypass position is often used for flushing fluid lines. The valve is turned
by a 1.8 stepper motor that has an encoder coupled to it for positioning feedback.
Figure 1-3 shows the components of a 3-port valve.
Figure 1-33-Port Valve Components
Functional Description of the XLP 6000
1 - Getting Started
1.4.3Printed Circuit Assembly
The printed circuit assembly (PCA) holds the microprocessor and circuitry to
control the syringe and valve drive. The PCA provides connectors for electrical
inputs and outputs as well as a communication address switch. For information on
modes of operation, see Chapter 3, "Software Communication".
Figure 1-4 shows the accessible components on the printed circuit board
assembly.
The XLP 6000 PCA has a DA-15 connector to handle power and communications.
For more information on the printed circuit assembly inputs/outputs and the
address switch, see Chapter 2, "Hardware Setup".
1.4.4Communication Interfaces
Depending on the pump configuration, the XLP 6000 can communicate singly or
in a multi-pump configuration through an RS-232, RS-485, or CAN (Controller
Area Network) interface. For RS-232 and RS-485, baud rates of 9600 and 38400
are supported. For CAN, baud rates of 100K,125K, 250K, 500K, and 1M are
supported.
For details on the communications interfaces, see Chapter 2, "Hardware Setup".
Up to fifteen (15) XLP 6000 pumps can be connected together in a multi-pump
configuration (also called “daisy-chaining”). In this configuration each pump is
addressed separately from a single terminal via its unique address, which is set
using the address switch on the back panel of the pump.
Within a multi-pump configuration, the RS-485 communications bus is required,
although the first pump in the chain may receive either RS-232 or RS-485
communications. For CAN communications, neither RS-232 nor RS-485 is
required. For more information on setting addresses, see Chapter 2.
1.5Safety
1 - Getting Started
Safety
The Cavro® XLP 6000 Modular Syringe Pump is designed for pipetting and
dispensing operations in the 5 µL to 25 mL range. Any other use is considered
improper and may result in damage to the pump and/or unreliable test results.
The XLP 6000 is designed to meet recognized technical regulations and is built
with state-of-the-art components. Nevertheless, risks to users, property and the
environment can arise when the module is used carelessly or improperly.
Appropriate warnings in this Operating Manual serve to make the user aware of
possible hazards.
1.5.1Notices and Symbols
Warning Notices Used in this Manual
The triangle warning symbol indicates the possibility of personal injury if the
instructions are not followed.
Specific symbols indicate the hazard to which a user is exposed. A few examples
follow.
Toxic Substance
Chemical or biological hazards can be associated with the substances used or the
samples processed with the XLP 6000. Always be aware of possible hazards
associated with these substances.
Explosion and Fire Hazard
Never process explosive or highly flammable liquids with the XLP 6000.
Automatically moving parts may cause injuries (crushing, piercing)
Attention
The general “Read This” symbol indicates the possibility of equipment damage,
malfunction or incorrect process results, if instructions are not followed.
1.6Tips for Setting Up the XLP 6000
For complete information on setting up the XLP 6000, see Chapter 2, "Hardware
Setup" and “Chapter 3, "Software Communication".
Note: Before performing any work with the XLP 6000, first read this Operating
Manual carefully.
To ensure proper operation, follow these tips:
Always set up and mount the pump in an upright position. Failure to do so can
cause problems priming the system.
Always run liquid through the syringe and valve when they are moving. Failure
to do so can damage the sealing surfaces.
Before running any organic solvents through the pump, see Appendix D,
"Chemical Resistance Chart" for more information on chemical compatibility.
Always power down the instrument when connecting or disconnecting pumps.
Caution! Keep fingers out of the syringe slot while the pump is running. Failure to
do so can cause injury.
This chapter includes these sections describing the various parts of hardware
setup:
Power and Electrical Considerations
Cabling
Communication Interfaces
Settings and Options
Installing Components
Mounting
2.1Power and Electrical Considerations
Power and Electrical Considerations
2 - Hardware Setup
The XLP 6000 requires a 24V DC power supply with a current rating of at least
1.5A, provided through a DA-15 connector. Tecan Systems recommends using
one power cable for every two pumps to provide noise immunity, i.e., power
should not be daisy-chained to more than two pumps.
2.1.1Choosing a Power Supply
The 24V DC supply for a single XLP 6000 should meet the following basic
requirements:
Output voltage: 24V nominal
Output voltage tolerance: ±10% maximum, ±5% preferred.
Well-regulated power supplies are recommended, as operating pumps below
24V will affect performance.
Output voltage regulation: ±1% with varying line (input voltage) and load
Output current (not including loads other than a single pump):
– 1.5A for power supplies with minimal capacitance.
– 850mA for power supplies with internal filter capacitance of at least 1000
F per amp of output current
– 850mA for power supplies with external capacitance of at least 1000 F
per amp of output current (aluminum electrolytic capacitor preferred)
Output voltage ripple: 50mV rms maximum at full load
Conformance to required safety and EMI/RFI specifications
Voltage turn-on and turn-off overshoot: < 2 volts
Minimum current load (for switchers): see “Switching Power Supplies” in this
chapter.
To meet the above basic requirements, the supply must incorporate either linear
or switching regulation and must have adequate output filter capacitance.
A current-limiting power supply is recommended. Current limiting above 1.0A is
acceptable, assuming that no additional equipment is operated from the supply.
If the power supply uses current feedback, the time-current foldback point must be
sufficient to allow charging of a 470 F capacitor without folding back. If an
external capacitor is used, exercise care to ensure that the supply always starts
after foldback, particularly at low AC line voltage.
2.1.2Integrating a Power Supply
When a power supply is used to operate more than one XLP 6000 or other device,
it must provide the total average current for all devices. The power supply and
filter capacitance together must satisfy the total peak input current for all devices.
For example, if a system incorporates six XLP 6000 pumps with other equipment
that together require 4 amps, a 10A power supply is satisfactory, provided the
output filter capacitance in the supply is at least 10,000 F:
6 x 0.85 = 5.1A; +4A = 9.1A (choose a 10A power supply)
If the power supply filter capacitance is less than 10,000 F, use either additional
external capacitance or a 15A power supply:
6 x 1.5 = 9.0A; +4A = 13A (choose a 15A power supply)
In this example, it is assumed that all the pumps and other equipment will
sometimes operate simultaneously.
External equipment with inadequate bypass capacitance or that is inadequately
sourced for current can cause overvoltage transients and sags, and can create
unnecessary ripple current in the XLP 6000. This can result in decreased
component life. Additionally , it is possible for a regulated power supply to become
unstable with certain loads and oscillate if adequate filter capacitance is not
present. Some forms of oscillation can cause failures in the XLP 6000. These
issues can be avoided by using a properly designed commercial power supply.
Consideration should also be given to the wiring of the XLP 6000 and any
additional devices. Wiring should be of sufficient gauge for the current, and as
short as possible. Unless otherwise required by safety requirements, the power
supply lines to the XLP 6000 should be 20AWG or heavier. Multiple XLP 6000s
can be daisy-chained, provided that the wire size and the power supply are
adequate for the total current. In the example of the six XLP 6000 pumps above,
use 18AWG wire if the units are daisy-chained. It is best if each pair is twisted or
dressed together from the device to the supply. For more information on multipump cabling, see Section 2.2, Cabling.
To control power to the XLP 6000, switch power to the power supply. Do not use a
relay or switch contacts between the 24V supply and the XLP 6000 (i.e., do not
switch DC input to the pump).
Be sure to check carefully the minimum load requirement of the power supply.
Typically, switching supplies have a minimum load requirement of up to 10% of
the rated output current.
Note: The XLP 6000 idle current is less than 10% of the full running current.
For example, in a system with multiple XLP 6000 pumps, a 24V 5-amp switcher
with a minimum load less that 500mA may not provide sufficient current when the
XLP 6000 motors are idle and all other devices are in a low current state. If the
XLP 6000 is the only load on the 24V supply, a switcher should have a minimum
load specification of 50mA or less. An appropriate external power resistor can be
used to ensure that the minimum load is met.
2 - Hardware Setup
Cabling
2.2Cabling
A single cable supplies both power and communications to each XLP 6000. A
unique address identifies each pump module. For more information, see “Address
Switch Settings” later in this chapter. See also Chapter 3, "Software
Communication".”
Power requirements are described in Section 2.1, Power and Electrical
Considerations.
Table 2-1DA-15 Connector Pin Assignments
PinFunctionRemarks
124V DC
2RS-232 TxD lineOutput data
3RS-232 RxD lineInput data
4Reserved
5CAN high signal line
6CAN low signal line
7Auxiliary input #1TTL level
8Auxiliary input #2TTL level
9GroundPower and logic
10GroundPower and logic
11RS-485 A lineData +
Figure 2-1 shows the pin positions of the DA-15 connector on the printed circuit
assembly. This is a male connector that requires a female connector on the
mating cable.
2.3Communication Interfaces
The computer or controller communicates with the XLP 6000 through an RS-232
interface, RS-485 interface, or CAN (Controller Area Network) interface.The XLP
6000 automatically detects the communication interface.
Examples of cabling connections are shown in Figure 2-2, Figure 2-3, and
Figure 2-4 on the following pages.
2.3.1RS-232/RS-485 Interface
The RS-232 interface automatically converts the protocol to RS-485 for the
benefit of any other devices which may be connected to the XLP 6000’s RS-485
communication bus (this constitutes the so-called “multi-drop” device
configuration).
Note: The RS-232 interface does not support hardware handshaking and
requires only three lines: RXD, TXD, and Signal Ground.
When using a multi-drop arrangement, up to 15 pumps can be addressed by the
controller on the same communications bus. Take special care to ensure that the
RS-485 A and B lines are not reversed. Refer to the cabling illustrations on the
following pages. These illustrations show the multi-pump cabling for RS-232, RS485, and CAN connections, respectively. Also shown is the external termination
scheme for the RS-485 chain.
2 - Hardware Setup
Communication Interfaces
2.3.2CAN Interface
The CAN interface is a two-wire serial system. The bus is driven differentially in a
manner similar to RS-485. The major difference is in the protocol. The CAN
protocol is designed to allow any device on the bus to send a message at any
time. This is unlike other two-wire interfaces in which the slave devices can only
transmit in response to a query. Using the CAN interface, the pump can send a
message to inform the master that it has completed its task. Anti-collision
detection (which reconciles problems that occur when two devices talk at once) is
carried out by the CAN controller hardware.
Caution! Always power off pumps before connecting to or disconnecting from the
bus.
The XLP 6000 firmware allows the user to configure the pump for different modes
of operation. The U commands (see Chapter 3, "Software Communication") are
used to write the configuration information to the non-volatile memory and control
the following options:
Valve type: The pump can be configured to operate with different valve
options (3-port, 4-port, T-valve, Y Block, 3-port distribution, 6-port distribution,
and 9-port distribution).
Baud rate: RS-232/RS-485 communication is possible at 9600 baud (default)
and 38400 baud. CAN communication is possible at 100K baud (default),
125K baud, 250K baud, 500K baud, and 1M baud rates.
Non-Volatile Memory Auto Mode: Allows the pump to run command strings
out of the non-volatile memory.
2 - Hardware Setup
Settings and Options
2.4.2Address Switch Settings
The address switch (see Figure 2-5, "Address Switch") is located near the top of
the XLP 6000 electronic circuit board. It is used to give each XLP 6000 in a multipump configuration a unique or specific address, allowing the user to direct
commands to specific pumps. The address switch has sixteen positions
(numbered 0 through F). Fifteen positions (addresses 0 through E) are valid pump
addresses.
To set the address switch, use a jeweler’s screwdriver or small flat head
screwdriver and turn the switch in either direction to the desired position.
Note: Power cycle (or power up) the pump after setting the address switch.
For information on the addressing schemes for different pump configurations, see
Chapter 3, "Software Communication".
2.4.3Self-Test
The “F” address switch position is used to activate the XLP 6000 self-test. Selftest causes the XLP 6000 to initialize, then cycle repeatedly through a series of
plunger movements. The self-test cycles through speed codes 0 to 14. If an error
condition occurs, the pump stops moving.
To run the self-test, set the address switch to position “F.” Then supply power to
the pump.
Caution! Always run liquid through the syringe and valve. Failure to do so can
damage the valve and syringe seal.
The XLP 6000 provides two auxiliary inputs and three auxiliary outputs that can be
accessed through the DA-15 connector, J5. They provide TTL level signals. The
outputs are controlled by the [J] command.
The auxiliary inputs are located on J5, pins 7 and 8. They can be read back using
report commands ?13 and ?14. Additionally, the inputs can be used to externally
trigger a command sequence using the [H] command. The commands are
described in Chapter 3, "Software Communication".
The auxiliary outputs are located on J5, pins 13, 14, and 15.
2.5Installing Components
2 - Hardware Setup
Installing Components
2.5.1Installing the XLP 6000 Valve
To install the XLP 6000 valve, follow these steps:
1Remove as much fluid as possible from the system by cycling the pump and
using air as the system fluid.
2Initialize the pump using the [ZR] command so that the valve motor shaft is in
the correct position.
3Issue an [A6000R] command to move the plunger to the bottom of travel.
4Remove the syringe and tubing.
5Remove the two socket head screws on the front of the valve, then remove
the valve from the pump. Do not remove the spacer.
6Install the new valve by placing it on the front panel so that the screw holes
line up. The valve coupler fitting mates to the valve motor shaft. The shaft
should be in the correct position. If it is not, re-initialize the pump using the
command [ZR].
7Replace the valve screws but do not tighten completely.
8Install the syringe and pull the syringe plunger until it is aligned with the
carriage. Align the valve using the plunger as a guide, and tighten from 1/4 to
1/2 turn after the screws contact the valve body.
9Pull the syringe plunger all the way into the carriage and secure by tightening
the plunger lock screw.
Caution! Be sure to reconfigure the pump firmware when changing valve types.
Failure to do so may damage the valve. See Section 3.3.2, Pump Configuration
Commands, for instructions on reconfiguring the pump.
1Remove the plunger lock screw.
2Install the syringe as shown in Figure 2-7, following these steps:
a. Screw the syringe into the valve.
b. Pull the syringe plunger down to the plunger holder assembly.
c. Align the plunger button through hole to the carriage mounting hole.
d. Slide the plunger lock screw through the plunger button and fasten to the
carriage.
Note: Make sure the plunger lock screw is securely tightened and the plunger
button is free to move on the plunger lock screw.