Sper Scientific 300005 User Manual

CLINICAL

REFRACTOMETER

300005

INSTRUCTION MANUAL

SPER
SCIENTIFIC

CONTENTS

Introduction ........................................................ 2

Description ......................................................... 2

Operating Procedures ........................................ 3

Precautions ........................................................ 4

Specifications ..................................................... 4

Warranty ............................................................ 4

Introduction

Your lightweight portable refractometer is a precision
optical instrument designed to measure urine specific
gravity (UG), serum albumen (SG / protein) and re­fractive index (Nd). It uses three accurate and gradu­ated scales that coincide with JSCP regulations. It is
excellent for medical monitoring, scientific research
and quality assurance requirements. Refractometers
operate on the principle that as the concentration or
density of a solution increases, its refractive index
changes proportionately. The refractive angle meas­ured by your refractometer registers on the scale. The
higher the concentration, the higher the readings reg­ister on the scale.

Description

Prism (1)

Calibration screw (2)

Cover Plate (3)

Barrel (4)

Eyepiece with focus ring (5)

- 2 -

Operating Procedures

1. With the COVER PLATE open, clean the PRISM
with a soft cloth to avoid scratching the surfaces.

2. Aim the refractometer toward a light source and
rotate the EYEPIECE to obtain the clearest focus.

3. Adjustment of the null (reference point):
A. Open the COVER PLATE.
B. Apply a few drops of pure distilled water onto

the PRISM platform.
C. Close the COVER PLATE.
D. Turn the CALIBRATION SCREW until the

dark and light boundary line coincides with the

bottom line of the scale (calibration line).

4. Carefully dry the prism platform and the cover.

5. Place a few drops of the test solution on the prism
and close the COVER PLATE so the solution
spreads evenly on the prism.

6. Aim the front of the refractometer towards the light
source and focus the eyepiece on the boundary
line of the light and dark hemispheres.

7. The boundary line indicates the concentration of
Urine Specific Gravity and Refractive Index of the
test sample.

Calibration

line

13600
80
60
40
20
13500
80
60
40
20
13400
80
60
40
13330

ND SERUM URINE Sp.G

–

—

–

—

–

—

12

—

–

—

–

—

–

—

11

—

—

–

—

–

–

—

10

—

–

—

–

—

–

—

9

—

–

—

–

—

–

—

8

—

–

—

–

—

–

—

–

—
—

–
–

—

–

—

–

—

–

—

–

—
—

–
–

—

–

—
—

–
–

—

–

—

–

—

–

—

–

—
—–

——

7

—

—

—

–

6

—

—

–

—

—

5

—

–

—

—

4

—

——

—

–

3

—

—

–

—

—

2

—

—

–

—

1

—

—

—–

——

0

—

___

—

W

JSCP

1.050

1.040

1.030

1.020

1.010

1.000

8. After use, clean prisms with a cloth and remove
any residue.

9. The temperature of the null reference liquid should
be at the same temperature as the sample solu­tion. For variations in temperature the null point
should be adjusted once every 30 minutes.

- 3 -

Precautions

Never submerge the unit, and do not let liquid to seep into
the unit’s body. Clean the refractometer after each use
with a soft cotton cloth. Do not scratch surface of the
prisms. Store in a dry, clean, and non-corrosive environ­ment. Avoid strong shocks.

Specifica­tions

Serum

Albumen

Range 1~12 g/dL 1.000 ~

Urine Spe-

cific Gravity

1.050

Refractive

Index

1.3330 ~

1.3600

Resolution 0.2 g/dL 0.002 0.0005

Accuracy

±0.2 g/dL ±0.002 ±0.0005
To ensure the highest accuracy,
perform calibration and testing at about
68~77°F (20~25°C).

Size 6 3/4” x 1 1/2" (171 x 38 mm)
Weight 3.1 oz (89 g)

Accesso­ries

Screwdriver, Carrying Case, Transfer Pi­pette, Distilled Water, Instruction Manual,
Registration Card.

WARRANTY

Sper Scientific warrants this product against defects in
materials and workmanship for a period of five (5)
years from the date of purchase, and agrees to repair
or replace any defective unit without charge. If your
model has since been discontinued, an equivalent Sper
Scientific product will be substituted if available. This
warranty does not cover damage resulting from
accident, misuse, or abuse of the product. To obtain
warranty service, ship the unit postage prepaid to:

SPER SCIENTIFIC

7720 E. Redfield Suite 7, Scottsdale, AZ 85260

WWW.SPERSCIENTIFIC.COM, [e-mail]

Please Note: The defective unit must be accompanied
by a description of the problem and your return address.
Register your product online or return your warranty
card within ten (10) days of purchase.

Rev. 10/9/06

- 4 -