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SUGAR / BRIX
REFRACTOMETER
300001, 300002
INSTRUCTION MANUAL
SPER
SCIENTIFIC
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CONTENTS
Introduction ............................................... 2
Description ................................................ 2
Operating Procedures ............................... 3
Precautions ............................................... 4
Specifications ............................................ 4
Warranty ................................................... 4
Introduction: Your portable refractometer is a
precision optical instrument designed to measure
the concentration of sugar in aqueous solutions.
It utilizes a Brix scale that is accurate and easy to
read. It is excellent for quality assurance, process control requirements, and research. Refractometers operate on the principle that as the concentration or density of a solution increases, its
refractive index changes proportionately. The refractive angle measured by your refractometer
registers on the scale. The larger the concentration of sugar in solution, the higher the reading on
the scale.
Description:
Prism (1)
Calibration screw (2)
Cover Plate (3)
Barrel (4)
Eyepiece with focus ring (5)
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Operating Procedures
1. With the COVER PLATE open, clean the PRISM
with a soft cloth to avoid scratching the surfaces.
2. Aim the refractometer toward a light source and
rotate the EYEPIECE to obtain the clearest focus.
3. Adjustment of the null (reference point):
A. Open the COVER PLATE.
B. Apply a few drops of pure distilled water onto
the PRISM platform.
C. Close the COVER PLATE.
D. Turn the CALIBRATION SCREW until the
dark and light boundary line coincides with the
bottom line of the scale.
4. Carefully dry the prism platform and the cover.
5. Place a few drops of the test solution on the prism
and close the COVER PLATE so the solution
spreads evenly on the prism.
6. Aim the front of the refractometer towards the light
source and focus the eyepiece on the boundary
line of the light and dark hemispheres.
7. The boundary line indicates the concentration of
sugar in the test sample.
15% Sample (300001) 43% Sample (300002)
——
——
—— 30
——
——
——
——
—— 25
—–-
——
——
——
—— 20
——
——
——
——
—— 15
——
——
——
——
—— 10
——
——
——
——
—— 5
——
——
——
——
—— 0
-
-
-
-60
-
-
-
-
55-
-
-
-
-
-50
-
-
-
-
45-
-
-
-
-
-40
-
-
-
-
35-
-
-
-
-
-30
-
-
8. After use, clean prisms with a cloth and remove
any residue.
9. The temperature of the null reference liquid should
be at the same temperature as the sample solution. For variations in temperature the null point
should be adjusted once every 30 minutes.
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Precautions
Never submerge the unit, and do not let liquid to seep
into the unit’s body. Clean the refractometer after each
use with a soft cotton cloth. Do not scratch surface of
the prisms. Store in a dry, clean, and non-corrosive
environment. Avoid strong shocks.
Specifications
300001 3000002
Range 0-32% 28-62%
Resolution 0.2%
Accuracy
Size 6 3/4” x 1 1/2"
(171 x 38 mm)
±0.2%
6 1/4” x 1 1/2"
(159 x 38 mm)
Weight 3.1 oz. (89 gr.) 3.2 oz. (90 gr.)
Accessories Screwdriver, Carrying Case, Trans-
fer Pipette, Distilled Water, Instruction Manual, Registration Card.
WARRANTY
Sper Scientific warrants this product against defects
in materials and workmanship for a period of five (5)
years from the date of purchase, and agrees to repair
or replace any defective unit without charge. If your
model has since been discontinued, an equivalent
Sper Scientific product will be substituted if available.
This warranty does not cover damage resulting from
accident, misuse, or abuse of the product. To obtain
warranty service, ship the unit postage prepaid to:
SPER SCIENTIFIC LTD,
7720 E. Redfield Suite 7
Scottsdale, AZ 85260
INFO@SPERSCIENTIFIC.COM
Please Note: The defective unit must be accompanied
by a description of the problem and your return
address. Register your product online or return your
warranty card within ten (10) days of purchase.
WWW.SPERSCIENTIFIC.COM
Rev. 08/31/04
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