Shimadzu LC-10, LC-20 User Manual

Clarity Controls

Shimadzu LC-10/20 System

LC + DET + AS + CO ENG

Code/Rev.: M108/70B

Date: 11/30/2016

Phone: [phone] DataApex Ltd.

Fax: [phone] Petrzilkova 2583/13

[e-mail] 158 00 Prague 5

www.dataapex.com The Czech Republic

Clarity®, DataApex®and

®

are trademarks of DataApex Ltd. Microsoft®and Windows

TM

are

trademarks of Microsoft Corporation.

DataApex reserves the right to make changes to manuals without prior notice. Updated manuals can be
downloaded from www.dataapex.com.

Author: DM

Shimadzu LC-10/20 System Table of Contents

Contents

1 Shimadzu LC-10/20 System 1
2 Requirements 3

2.1 Minimal version of device firmware required 4

3 Installation Procedure 5

3.1 Shimadzu LC-10/20 System communication 5

3.2 Software Installation 7

3.3 Clarity Configuration 8

3.4 Shimadzu LC-10/20 System Setup 11

3.4.1 Shimadzu LC-10/20 System Setup - Setup 12

3.4.2 Shimadzu LC-10/20 System Setup - Controller 14

4 Using the control module 16

4.1 Pump 17

4.1.1 Shimadzu LC-10/20 System Setup - Pump 17

4.1.2 Method Setup - LC Gradient 19

4.1.2.1 Gradient Options 21

4.1.3 Method Setup - LC 23

4.1.4 Method Setup - Advanced 26

4.1.5 Device Monitor 27

4.1.6 Report Setup 29

4.2 Detector 30

4.2.1 Shimadzu LC-10/20 System Setup - Detector 30

4.2.2 Method Setup - Acquisition - Detector 32

4.2.2.1 Method Setup - Acquisition - RF Detector 34

4.2.2.2 Method Setup - Acquisition - RID Detector 36

4.2.3 Method Setup - Acquisition - Time Program 38

4.2.4 Method Setup - Acquisition - Analog Output 40

4.2.5 Device Monitor 41

4.2.6 Report Setup 43

4.3 Autosampler 45

4.3.1 Shimadzu LC-10/20 System Setup - Autosampler 45

4.3.2 Method Setup - AS - Sampler 47

4.3.2.1 Method Setup - AS - Sampler (SIL-10Axl) 49

4.3.2.2 Method Setup - AS - Sampler (SIL-10AF) 50

4.3.3 Method Setup - AS - Time Program 51

4.3.4 Method Setup - AS - Pretreatment 52

4.3.5 Device Monitor - Shimadzu LC-10/20 System 56

4.3.6 Report Setup 57

4.4 PDA 58

4.4.1 Shimadzu LC-10/20 System Setup - PDA Detector 58

4.4.2 Method Setup - PDA 60

4.4.3 Method Setup - Acquisition 62

4.4.4 Device Monitor 63

4.4.5 Report Setup 64

4.5 Thermostat 65

- i -

Table of Contents Clarity Controls

4.5.1 Shimadzu LC-10/20 System Setup - Thermostat 65

4.5.2 Method Setup - Thermostat - Thermostat 66

4.5.3 Method Setup - Thermostat - Time Program 67

4.5.4 Device Monitor 68

4.5.5 Report Setup 69

5 Troubleshooting 70
6 Vial Plate Numbers 71

- ii -

Shimadzu LC-10/20 System Table of Contents

To facilitate the orientation in the Shimadzu LC- 10/20 System manual and Clarity
chromatography station, different fonts are used throughout the manual. Meanings of these fonts
are:

Instrument (blue text) marks the name of the window to which the text refers.

Open File(italics) describes the commands and names of fields in Clarity, parameters that can
be entered into them or a window or dialog name (when you already are in the topic describing
the window).

WORK1 (capitals) indicates the name of the fileand/or directory.
ACTIVE (capital italics) marks the state of the station or its part.

The bold text is sometimes also used for important parts of the text and the name of the Clarity
station. Moreover, some sections are written in format other than normal text. These sections are
formatted as follows:

Note: Notifies the reader of relevant information.

Caution: Warns the user of possibly dangerous or very important

information.

▌ Marks the problem statement or trouble question.

Description: Presents more detailed information on the problem, describes its causes,

etc.

Solution: Marks the response to the question, presents a procedure how to remove it.

- iii -

Shimadzu LC-10/20 System 1 Shimadzu LC-10/20 System

1 Shimadzu LC-10/20 System

This manual describes the setting of the Shimadzu LC-10/20 System.
The control module enables direct control of the instrument over serial line
or LAN (in case of LC-20 series).

Caution: The Shimadzu LC-10/20 System control module can not be combined

with Shimadzu LC- 10/20 Pump control module on a single PC where
Microsoft .NET Framework is installed.

Caution: A single Clarity station can only control one Shimadzu LC- 10/20

System. This is the restriction of MIMIC libraries used to perform the

instrument control.

Fig 1: Shimadzu Prominence system

As opposed to the Shimadzu LC- 10/20 Pump , this control module
operates the whole system (including detectors, autosampler, thermostat
etc.), not just the pump, but requires any of the supported controllers for
the operation of any other modules. Direct control means that the system
can be completely controlled from the Clarity environment. Instrument
method controlling the analysis conditions will be saved in the measured
chromatograms. Clarity can currently control following modules:

Controllers: SCL-10Avp, CBM-20A, CBM-20ALite
Pumps: LC- 6AD, LC-8A, LC-10AD, LC-10AS, LC- 10AT, LC- 10Ai, LC-

10ADvp, LC-10ATvp, LC-20AT*, LC-20AD*, LC-20ADXR*, LC-20AB*, LC­20AS*
Detectors: SPD- 10A, SPD- 10Ai, SPD- 10AV, SPD- 10Avi, SPD- 10Avp,
SPD-10AVvp, SPD-20A*, SPD-20AV*, RID-10A, RID-20A**, RF-10A, RF­10Axl, RF-20A**, RF-20Axs**
Autosamplers: SIL- 10ADvp, SIL- 10AF, SIL- 10Axl, SIL- HTA, SIL- HTC,
SIL- 20A*, SIL- 20AC*, SIL- 20AHT*°, SIL- 20ACHT*°, SIL- 20AXR*, SIL­20ACXR*
PDA Detectors: SPD-M10Avp***, SPD-M20A*

- 1 -

1 Shimadzu LC-10/20 System Clarity Controls

Thermostats: CTO-10A, CTO-10AC, CTO- 10Avp, CTO-10ACvp, CTO­10ASvp, CTO-20A*, CTO-20AC*
The list continues to extend, for up to date list see the website
www.dataapex.com. Some devices also require a firmware of particular
minimal version for proper communication with Clarity(for more details
see the chapter Minimal version of device firmware required on pg 4).
* Devices marked with the asterisk will show in the System Configuration
dialog only after the CBM-20A (Lite) controller has been selected.

Caution: If any device from the LC-20 (Prominence) line is to be used with the

LC-10 line Controller, it is necessary to set it to the LC- 10 emulation
mode. This is done (for example on the LC-20AD pump) on the devices

display by pressing the VP key until the CALIBRATION screen appears.
Then press func key, enter the password and keep pressing the func
key until OP MODE screen shows. There, select the correct type of the
controller installed. Later in the System Configuration dialog it is
necessary to set the LC-20AD pump as LC-10AD when selecting the type
of the pump in the Shimadzu LC-10/20 System setup.

° The autosamplers SIL-20AHT and SIL-20ACHT marked with the circle
do not possess their specific option in the Shimadzu LC- 10/20 System
Autosampler dialog. In case of SIL-20AHT autosampler is present it
should be configured through SIL- 20A option. If the SIL- 20ACHT
autosampler is present it is necessary to configure it through SIL-20AC
option.
** RID-20A, RF-20A and RF-20AXS detectors must be always switched to
the LC- 10 emulation mode in order to be controlled (using MIMIC
libraries) in Clarity.
*** SPD-M10Avp PDA detector is not supported on 64-bit Windows.
RF-10AXL and RF-20AXS ( RF-20AXS with minimum firmware version

2.02) fluorescence detectors can be configured as a stand-alone control
module developed through Knauer company. This control module for RF-
10AXL and RF- 20AXS fluorescence detectors supports serial
communication via special RS232 cable (p/n SK 11) . If the RF-20A
detector was purchased through Knauer company (with special oem
firmware version) it can be connected to Clarity with above mentioned
special RS232 serial cable and controlled with above mentioned specific
control module.
Clarity does not control following controllers: CBM-10A, SCL-10A.

- 2 -

Shimadzu LC-10/20 System 2 Requirements

2 Requirements

l Clarity installation CD ROM with LC control module (p/n A24) allowed.

Other parts of the system may need other modules or extensions, namely
the autosampler will need the AS control module (p/n A26) and the PDA
Detector will need the PDA extension (p/n A29) to operate.

l Supported Shimadzu system controller (see the full list of supported

controllers in the chapter "Shimadzu LC-10/20 System" on pg 1.). The
controller is needed even for the control of standalone PDA detector.

l Free serial port in the PC.

Note: Modern computers usually have only 1 (if any) serial (COM) port installed.

To use more devices requiring the RS232 port, the MultiCOM adapter
(p/n MC01) isavailable.

l Standard RS232-compliant cross DB9F-DB9F serial cable (p/n SK01) or

cross LAN cable (p/n SK08) in case of LC-20 system.

Note: Cables are not part of Clarity Control Module. It is strongly

recommended to order required cables together with the Control Module.

l SCSI card for use of the SPD- M10Avp PDA detector. SPD-M20A PDA

detector uses LAN communication.

Caution: It is recommended to use Adaptec SCSIcards. Drivers for Adaptec's

cards are located on Clarity installation CD ROM in directory HW_
DRIVERS\ASPI2 . Read the README.TXT file before you install the
drivers.

Caution: SPD-M10Avp PDA detector is not supported on 64-bit Windows.

- 3 -

2 Requirements Clarity Controls

2.1 Minimal version of device firmware required

Tab 1: Controllers :

Name of Device: Minimal version of firmware required:

SCL-10Avp 5.33
Subcontroller 5.20
OptionBox-L 3.2

Tab 2: Pumps :

Name of Device: Minimal version of firmware required:

LC-10ADvp 5.26
LC-10ATvp 5.27
LC-10Ai 3.1
LC-10AD 3.1
LC-10AT 3.1
LC-10AS 3.1
LC-8A 1.5
LC-6AD 1.4

Tab 3: Autosamplers :

Name of Device: Minimal version of firmware required:

SIL-10ADvp 5.32
SIL-HTA 6.02
SIL-HTC 6.02

Tab 4: Column Ovens :

Name of Device: Minimal version of firmware required:

CTO-10Avp 5.24
CTO-10ACvp 5.24
CTO-10ASvp 5.24
CTO-10A[C] 3.0

Tab 5: Detectors :

Name of Device: Minimal version of firmware required:

SPD-10Avp 5.22
SPD-10AVvp 5.22
SPD-10Ai 3.0
SPD-10Avi 3.0
SPD-10A 3.0
SPD-10AV 3.0
RF-20A, Axs 1.10

- 4 -

Shimadzu LC-10/20 System 3 Installation Procedure

3 Installation Procedure

3.1 Shimadzu LC-10/20 System communication

Shimadzu LC-10/20 System may be controlled either via standard serial

cross DB9F-DB9F cable or via cross LAN cable, depending on the type of
connector present on the controller. LAN communication is only present
on LC-20 line controllers.

Serial Connection

The Shimadzu LC- 10/20 System is controlled by serial (RS232)
communication. It uses standard serial cross DB9F-DB9F cable described
in the picture.

Fig 2: Serial cross DB9F - DB9F cable

LAN connection

For direct connection between chromatograph and computer, the cross
LAN cable should be used. On the other hand, for the connection to the
network, the straight LAN cable might be necessary (with older switches)
instead.

Shimadzu SCL-10Avp settings

In the controller it is necessary to set the Classvp parameter (accessible
from the main menu via the system submenu (key 4, it is located on the
bottom of the list) to 5.x value. Other communication parameters set in the
controller should be:

Interface: RS-232C
Baud Rate: 19200
Level: Enhanced

Caution: Only COM ports up to COM9 are supported. Serial ports with higher

numbers cannot be used. This also means that the Shimadzu LC-10/20
System cannot be connected to the serial port on Net- PAD (COM101

and COM102).

- 5 -

3 Installation Procedure Clarity Controls

LC-20 components with SCL-10Avp controller

Shimadzu LC- 20 components have to be set to the LC-10 emulation

mode when they should be working under the LC-10 controller. This is
done from the device's keyboard according to this procedure (procedure
presented on the LC-20 series pump, may differ slightly for other devices):

l Press the VP button on the devices's front keyboard until the

CALIBRATION inscription is shown on the display.

l Press the func button to enter the configuration. The password has to be

filled in.

Note: The default password is 00000.

l Press the func button repeatedly until the OP MODE inscription is shown

on the display.

l Enter the desired number (1 for the pump) to switch the device to the SCL-

10Avp emulation mode and press the enter button.

l Switch the device off and on again. The new communication parameters

should be saved and used.

The procedure is in detail described in the LC- 20 hardware manual
provided by the Shimadzu company.

CBM-20A(Lite) settings

It is necessary to set the communication parameters in the controller. From

Clarity version 2.6.5, the parameters are:
Communication Mode: ClassVP
Interface: RS232/Ethernet (depending on the desired connection type)

The exact procedure is described in the controllers hardware manual.

- 6 -

Shimadzu LC-10/20 System 3 Installation Procedure

3.2 Software Installation

Caution: If the PDA detector is to be used, it is necessary to switch it on before the

computer is turned on. This way, the SCSI controller will be detected
automatically. When turned on later, the PDA has to be added manually
using the Add Hardware function from the Windows Control Panel.

Install (or re-install) the Clarity software from the installation CD.

l Insert the installation CD into the CD-ROM of the computer and start the

installation.

l Select the target directory and press the Next button.
l Select Custom installation. The control module for the Shimadzu LC-

10/20 System is automatically included in it.

l If the PDA detector is to be used, select the Detector Control option (its

icon is checked, but grayed) and either click the checkbox to change it to
checked (not grayed) state or press the Details… button and check the
Shimadzu PDA option there.

l Follow the instructions given by the installation procedure until the

installation procedure ends.

- 7 -

3 Installation Procedure Clarity Controls

3.3 Clarity Configuration

Caution: If the PDA detector will be used, it must be switched on prior to starting the

computer.

Fig 3: System Configuration

l

Start the Clarity station by clicking on the icon on the desktop.

l Invoke the System Configuration dialog accessible from the Clarity

window using the System - Configuration… command.

- 8 -

Shimadzu LC-10/20 System 3 Installation Procedure

l Press the Add button ① (see Fig 3 on pg 8 .) to invoke the Available

Control Modules dialog.

l You can specify the searching filter ② to simplify the finding of the driver.
l Select the LC-10/20 System item and press the Add ③ button.

The Shimadzu LC-10/20 System Setup dialog will appear.

Fig 4: Shimadzu LC-10/20 System Setup

l Select the type of the controller in the Model field and the Serial Port or IP

Address to which the controller is connected.

Note: Only CBM- 20A and CBM- 20ALite controllers may be connected

through LAN.

l Select other parts of the system that are connected to the controller (such

as pumps, detectors, autosampler etc.). Each module selected will add its
own tab to the Shimadzu LC 10/20 System Setup dialog.

Note: Other fields and parameters of the Shimadzu LC- 10/20 System Setup

dialog are described later in the manual(for more details see the chapter
Shimadzu LC-10/20 System Setup - Setup on pg 12).

l Set the Instrument Type on the desired Instrument tab ④ to LC (or LC-

PDA when you want to use the PDA detector).

l Drag and drop the LC- 10/20 System icon from the Setup Control

Modules ④ list on the left side of the System Configuration dialog to the

desired Instrument ⑤ tab on the right side ⑥ (or use the button ⑦ to
do so).

- 9 -

3 Installation Procedure Clarity Controls

Note: When you only change some of the already configured parts of the

Shimadzu LC-10/20 System, it is recommended to remove the system
from the given Instrument tab and add it there once more.

l Set the Ext. Start Dig. Input and Ready Dig. Output numbers ⑧ for your

acquisition card according to the wires being used for synchronization.

Caution: The Shimadzu LC- 10/20 System must have all subdevices configured

on the same Instrument (cannot have parts of it on different Instruments)
and no sub- device may be left unconfigured (if any subdevice is
configured on the Instrument, all subdevices must be).

- 10 -

Shimadzu LC-10/20 System 3 Installation Procedure

3.4 Shimadzu LC-10/20 System Setup

Shimadzu LC-10/20 Pump Setup dialog (accessible through the System
Configuration dialog) is used to set the connection to the Shimadzu LC-

10/20 System , select it's configuration and set some other basic
parameters. The Setup tab is always opened by default.

Digital Input Names

Pressing the Change button opens the Digital Input Names dialog which
allows to set the custom name for the virtual Shimadzu LC-10/20 System
digital input. This input allows to start Clarity run by the signal of the
Shimadzu LC-10/20 System controller.

Fig 5: Digital Input Names

- 11 -

3 Installation Procedure Clarity Controls

3.4.1 Shimadzu LC-10/20 System Setup - Setup

Fig 6: Shimadzu LC-10/20 System Setup - Setup

System Controller

Section defining the type of the Shimadzu LC-10/20 System controller
and the connection to it.

Model

Sets the type of the controller unit. Only SCL-10Avp, CBM- 20A and
CBM-20ALite controllers are supported at the moment.

Note: The selection of the controller influences the number of options that will be

displayed when configuring other system devices. If any LC-20 modules
should be used with the SCL-10Avp controller, these devices have to be
switched to the LC- 10 emulation mode (for more details see the
chapter Installation Procedure on pg 5).

Serial Port

Selects the serial port to which the Shimadzu LC-10/20 System is
connected.

IP Address

Sets the IP Address if using the LAN connection. LAN can only be
used for the Shimadzu LC-20 controllers.

Connect

Tests the connection to the controller. If it is correctly set, the Hardware

Configuration dialog with all controller information and other

information on added modules will appear.

- 12 -

Shimadzu LC-10/20 System 3 Installation Procedure

Fig 7: Hardware Configuration

Pumps

Sets the model of the pump connected. Up to three pumps may be
configured in a Shimadzu LC-10 and four pumps on Shimadzu LC-20
System.
The first pump may have valves configured, other pumps are not allowed
to have any valves. Any pump may be set as gradient or auxiliary, but
maximum of four solvents can be used in a gradient, so any further pumps
are automatically configured as auxiliary.

Detectors

Sets the model of the detector connected. Up to two detectors may be
configured in a Shimadzu LC-10/20 System.

Autosampler

Sets the model of the autosampler.

PDA Detector

Set the model of the PDA detector.

Thermostat

Sets the model of the thermostat.

Note: It is recommended to add the modules one by one and to check the

communication with the Connect button in between.

- 13 -

3 Installation Procedure Clarity Controls

3.4.2 Shimadzu LC-10/20 System Setup - Controller

Fig 8: Shimadzu LC-10/20 System Setup - Controller

Switches

System Protect

While checked, in case of exceeding the maximal pressure the flow
rate will be decreased to one half of the set value. If unchecked, the
exceeding will shut-off the pump.

Sound Alarm on Error

Check to signalize error messages by sound.

Turn Off Relays on Error

If checked, in case of the controller error the AC power for devices
plugged in the multiple terminal box will be turned off.

Autozero Detector A (B)

If checked, the particular detector response will be set to zero at the
start of acquisition.

Autozero PDA

If checked, the PDA detector response will be set to zero at the start of
acquisition.

Relays

Relay 1 (..4)

These controls define the trigger criterion for each controller relay
outputs. Possible criteria are:

Start - Relay closed at the beginning of the analysis.
Stop - Relay closed at the end of the analysis or time program.
Error - Relay closed in the event of the system controller error.
Event - Relay closed on the time basis as the event of the HPLC time

program.

- 14 -

Shimadzu LC-10/20 System 3 Installation Procedure

External Start

This control sets the conditions for closing the controllers start relays at
the beginning of the analysis. Possible options are:

Disable - External start is disabled.
All Runs - Closes start relays at each analysis start including starts

with no injection.
Inject Only - Closes start relays only after starting the analysis with the
injection performed.

- 15 -

Shimadzu LC-10/20 System 4 Using the control module

4 Using the control module

Several new tabs appear in the Method Setup dialog, based on the
settings performed in the Shimadzu LC-10/20 System Setup dialog. These
new tabs enable the setting of the Shimadzu system operation program.

Note: The instrument method is always sent to the Shimadzu LC- 10/20

System as a whole.

- 16 -

4 Using the control module Clarity Controls

4.1 Pump

4.1.1 Shimadzu LC-10/20 System Setup - Pump

This tab of the Shimadzu LC-10/20 System Setup dialog allows to set the
parameters of the given pump.

Fig 9: Shimadzu LC-10/20 System Setup - Pump

Auxiliary Pump

Check for the pump to become auxiliary pump (not part of the Gradient
Table). If there are more pumps added in the configuration and the first

one is set either as low- pressure gradient or binary pump, the other
pumps are automatically set as Auxiliary pumps.

Valve Model

Select the valve model installed in your pump. This selection influences
the number of solvents available.

Solvents

Select the number of solvents to be used.

Note: Only the first pump configured as a gradient pump allows to set the

number of solvents in the Low- pressure gradient. Available options
depend on the Valve Model configured.

Solvent 1 (..4) Name

Change names for the particular solvent.

Low Pressure Limit

Lower pressure limit. Number entered will be sent to the pump and
checked by the pump firmware.

- 17 -

Shimadzu LC-10/20 System 4 Using the control module

High Pressure Limit

Upper pressure limit. Number entered will be sent to the pump and
checked by the pump firmware.

Note: Exceeding of High Pressure Limit or Low Pressure Limit defined here will

cause a system error, requiring the closure and reopening of the given
Instrument.

- 18 -