ION570
Ion Analyser
D21M073
Reference Manual
D21M073 • Printed by Radiometer Analytical SAS • 2005-02C
MeterLab
®
ION570 Reference Manual
Contents
Contents..................................................................................................................3
Introduction ..........................................................................................................13
Read me! ...............................................................................................................14
Practical examples ..............................................................................................17
Programming electrodes ...........................................................................................19
Programming reagents ..............................................................................................25
Programming methods ..............................................................................................27
Programming sequences ...........................................................................................31
Programming tips ................................................................................................33
Glossary ...............................................................................................................35
ABU1/ABU2 .................................................................................................................37
Accept a result ............................................................................................................37
Acceptance criteria ....................................................................................................38
Acceptation .................................................................................................................39
Access routine mode .................................................................................................39
Active electrode unknown in "method ID" ...............................................................39
Add method menu ......................................................................................................40
Addition .......................................................................................................................40
Addition delay .............................................................................................................41
Addition method - definition .....................................................................................42
Addition method - programmation ...........................................................................43
Addition volume .........................................................................................................44
Address .......................................................................................................................44
Alarm: Locked .............................................................................................................44
Alarm: Unlocked .........................................................................................................45
Aliquot .........................................................................................................................45
Alphanumeric characters ..........................................................................................46
Applied signal (AC/DC) ..............................................................................................46
Archives data lost - Cal. Data lost - Methods kept ..................................................46
Archiving .....................................................................................................................47
Assistant function ......................................................................................................47
Automatic (calibration mode) ....................................................................................47
Autochaining ...............................................................................................................48
Auxiliary input .............................................................................................................49
Auxiliary output ..........................................................................................................50
Aux. on for ...................................................................................................................50
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Bar code reader connection ......................................................................................50
Batch number .............................................................................................................50
Beaker menu ...............................................................................................................51
Beakers: [F;L] .............................................................................................................51
Beep .............................................................................................................................52
Burette functions menu .............................................................................................52
Burette speed ..............................................................................................................52
Burette volume ...........................................................................................................52
C0 (Detection limit) .....................................................................................................52
Cable capacity ............................................................................................................53
Cable resistance .........................................................................................................54
Calibrate conductivity cells .......................................................................................55
Calibrate ISE electrodes ............................................................................................55
Calibrate pH electrodes .............................................................................................55
Calibration = Automatic .............................................................................................56
Calibration = Manual ..................................................................................................57
Calibration curve of an ISE electrode .......................................................................58
Calibration delay elapsed ..........................................................................................58
Calibration parameters ..............................................................................................58
Calibration point menu ..............................................................................................59
Calibration request/Calibration .................................................................................60
Calibration request = Fixed .......................................................................................61
Calibration request = Free .........................................................................................62
Calibration stack .........................................................................................................62
Calibration results parameters ..................................................................................62
Catalogue list ..............................................................................................................62
Cell constant (parameter) ..........................................................................................63
Cell constant (definition) ...........................................................................................64
Cell grounding ............................................................................................................64
Cell window .................................................................................................................65
Change electrode name .............................................................................................65
Change method name ................................................................................................65
Change reagent name ................................................................................................65
Change sequence name ............................................................................................65
Check command .........................................................................................................66
Check electrodes ........................................................................................................66
Check reagents ...........................................................................................................67
Conc. 1 too high compared to reagent concentration ............................................67
Conc. n too high compared to reagent concentration ............................................68
Conc. not increasing in “method ID“ ........................................................................68
Concentration x ..........................................................................................................69
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Concentration unit ......................................................................................................69
Conductivity cell .........................................................................................................69
Conductivity cell calibration ......................................................................................69
Conductivity measurement method .........................................................................70
Configuration menu ...................................................................................................71
Connections ................................................................................................................71
Connect electrodes ....................................................................................................71
Contrast .......................................................................................................................72
Controlled by TTL IN ..................................................................................................72
Copy electrode ............................................................................................................73
Copy method ...............................................................................................................74
Copy reagent ...............................................................................................................74
Coupled method .........................................................................................................75
Create electrode .........................................................................................................76
Create method ............................................................................................................77
Create reagent ............................................................................................................77
Current value ..............................................................................................................77
Curve ...........................................................................................................................78
Curves data lost - Cal. Data kept - Methods kept ....................................................78
Customise ...................................................................................................................78
Date entry ....................................................................................................................78
Default parameters .....................................................................................................79
Delay after addition ....................................................................................................79
Delete electrode ..........................................................................................................79
Delete method .............................................................................................................79
Delete reagent .............................................................................................................80
Demand: Locked .........................................................................................................80
Demand: Unlocked .....................................................................................................81
Detailed ........................................................................................................................82
Detection limit (C0) .....................................................................................................82
Dilution (Yes/No) .........................................................................................................82
Direct ISE measurement method - definition ...........................................................83
Direct ISE measurement method - notes .................................................................84
Direct ISE measurement method - programmation .................................................84
Direct measurements .................................................................................................84
Disconnect electrodes ...............................................................................................85
Display contrast ..........................................................................................................85
Display measurement ................................................................................................86
E0 standard potential .................................................................................................86
EC socket ....................................................................................................................87
EC/pH measurement method - definition .................................................................88
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EC/pH measurement method - programmation .......................................................89
Edit electrode menu ...................................................................................................90
Edit method menu ......................................................................................................91
Edit reagent menu ......................................................................................................92
Edit sequence menu ...................................................................................................93
Electrode calibration (Fixed mode, conductivity cell) .............................................94
Electrode calibration (Free mode, conductivity cell) ..............................................95
Electrode calibration (ISE) .........................................................................................96
Electrode calibration (Fixed mode, pH electrode) ...................................................97
Electrode calibration (Free mode, pH electrode) .....................................................98
Electrode calibration (sequence) ..............................................................................99
Electrode calibration not required ............................................................................99
Electrode calibration parameters ............................................................................100
Electrode calibration stack ......................................................................................101
Electrode connection ...............................................................................................102
Electrode connection - Important ...........................................................................103
Electrode function ....................................................................................................103
Electrode icons .........................................................................................................104
Electrode ID ...............................................................................................................104
Electrode library .......................................................................................................105
Electrode not calibrated ...........................................................................................105
Electrode system ......................................................................................................105
Electrode type ...........................................................................................................106
Electrode window .....................................................................................................107
Empty burette ...........................................................................................................107
Empty sequence .......................................................................................................107
Enter titre ...................................................................................................................108
Error - Error messages ............................................................................................109
Expiry date ................................................................................................................109
Expiry date elapsed ..................................................................................................110
Fill burette .................................................................................................................110
Final dil. amount .......................................................................................................110
Fixed (calibration mode) ..........................................................................................110
Flush burette .............................................................................................................111
Format (printouts) ....................................................................................................111
Free beakers .............................................................................................................112
Free (calibration mode) ............................................................................................112
Function ....................................................................................................................112
Fuses .........................................................................................................................113
Global flush burettes ................................................................................................114
GLP-Archives menu .................................................................................................115
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GRAN = f(Volume) curve ..........................................................................................116
Ground conflict .........................................................................................................117
Help ............................................................................................................................117
High (result indicator) ..............................................................................................117
Icons ..........................................................................................................................117
ID ................................................................................................................................118
Initial volume .............................................................................................................118
Input address conflict ..............................................................................................118
Insert method menu .................................................................................................119
Install burette ............................................................................................................119
Install reagent ...........................................................................................................120
Insufficient number of beakers ...............................................................................120
ION cell external Gnd ...............................................................................................121
ISE calibration results parameters .........................................................................121
ISE calibration solutions ..........................................................................................121
ISE Standard addition method - definition .............................................................122
ISE Standard addition method - programmation ...................................................123
Iso pH .........................................................................................................................124
Keyboard connection ...............................................................................................125
Keyboard connection - Important ...........................................................................126
Language ...................................................................................................................126
Linear (temperature correction) ..............................................................................126
Low (result indicator) ...............................................................................................126
Main window .............................................................................................................127
Mains frequency .......................................................................................................127
Maintenance ..............................................................................................................128
Manual (calibration mode) .......................................................................................128
Manual dosing ..........................................................................................................129
Max. stab reached ....................................................................................................129
Max. stab time ...........................................................................................................130
Measurement ............................................................................................................130
Measurement method ..............................................................................................131
Method .......................................................................................................................131
Method library ...........................................................................................................132
Method parameters menu ........................................................................................132
Method results menu ...............................................................................................133
Method wrong type ...................................................................................................133
Min. cell cst - Max. cell cst .......................................................................................133
Min. pH0(25) - Max. pH0(25) .....................................................................................134
Min. sensitivity - Max. sensitivity ............................................................................134
Min. Temp. - Max. Temp. ..........................................................................................134
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Minimum value - Maximum value ............................................................................135
Mode ..........................................................................................................................135
Molar weight ..............................................................................................................135
Nat. water (temperature correction) ........................................................................136
Nb lines per page (printouts) ...................................................................................136
None (temperature correction) ................................................................................136
Notification message ...............................................................................................136
Number of additions .................................................................................................136
Number of beakers ...................................................................................................137
Number of buffers ....................................................................................................137
Number of cycles ......................................................................................................137
Number of decimals .................................................................................................137
Number of digits .......................................................................................................138
Number of rinses ......................................................................................................138
Number of solutions .................................................................................................138
Number of tests ........................................................................................................139
OK (result indicator) .................................................................................................139
Others list ..................................................................................................................139
Parameters menu .....................................................................................................139
PC cable - A95X501 ..................................................................................................140
PC connection ..........................................................................................................140
PC keyboard ..............................................................................................................140
Periodicity .................................................................................................................141
Periodicity for QC samples ......................................................................................141
pH0(25) ......................................................................................................................141
pH buffer ....................................................................................................................141
pH int .........................................................................................................................142
Potential jump ...........................................................................................................142
Potential versus SHE ...............................................................................................143
Preprogrammed list ..................................................................................................143
Printer ........................................................................................................................144
Printer cables - A95P201, A95X506 .........................................................................145
Printer connection ....................................................................................................146
Print in table ..............................................................................................................146
Printouts ....................................................................................................................147
Printouts detailed .....................................................................................................147
Printouts menu .........................................................................................................148
Printouts setup .........................................................................................................148
Printouts title ............................................................................................................149
Programming method ..............................................................................................149
Programming sequence ...........................................................................................150
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QC (result indicator) .................................................................................................151
QC analysis required ................................................................................................151
QC data menu ...........................................................................................................151
QC ID ..........................................................................................................................151
QC not required ........................................................................................................151
QC periodicity elapsed .............................................................................................152
QC sample .................................................................................................................152
QC sample (Yes/No) .................................................................................................152
Reagent addition ID .................................................................................................152
Reagent addition volume .........................................................................................153
Reagent icons ...........................................................................................................153
Reagent ID .................................................................................................................154
Reagent library .........................................................................................................154
Reagent system ........................................................................................................154
Reagent titre not entered .........................................................................................154
Reagent unit ..............................................................................................................155
Reagent window .......................................................................................................155
Recalculate results ...................................................................................................156
Ref. electrode conflict ..............................................................................................157
Reference Temp. .......................................................................................................157
Reject a result ...........................................................................................................157
Remove burette ........................................................................................................157
Remove method from a sequence ..........................................................................157
Remove reagent ........................................................................................................157
Replace burette .........................................................................................................158
Replace electrodes ...................................................................................................158
Replace reagent ........................................................................................................159
Replacing reagent bottle ..........................................................................................159
Reset memory ...........................................................................................................160
Reset to factory settings ..........................................................................................160
Result accepted (Yes/No) ........................................................................................161
Result indicators ......................................................................................................162
Result unit .................................................................................................................162
Results .......................................................................................................................163
Results factor (Yes/No) ............................................................................................164
Results menu ............................................................................................................164
Rinse aux. output .....................................................................................................165
Rinse burette .............................................................................................................165
Rinse time .................................................................................................................165
Routine mode ............................................................................................................166
Run window ..............................................................................................................167
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Run window (continued) ..........................................................................................168
Running a manual dosing ........................................................................................169
Running a method/sequence ..................................................................................170
SAC80/SAC90 ...........................................................................................................171
SAC80/SAC90 cable - A95A202 ...............................................................................171
SAC ext. cell GND .....................................................................................................171
SAC Method ..............................................................................................................171
SAC Sequence ..........................................................................................................171
Same buffer change buffer ......................................................................................172
Sample amount .........................................................................................................172
Sample changer ........................................................................................................172
Sample dilution conflict ...........................................................................................172
Sample ID ..................................................................................................................173
Sample menu ............................................................................................................173
Sample stack .............................................................................................................174
Sample unit ...............................................................................................................174
Sample unit conflict .................................................................................................175
Select electrode ........................................................................................................175
Select method ...........................................................................................................175
Select reagent ...........................................................................................................176
Select sequence .......................................................................................................176
Sensitivity ..................................................................................................................176
Sequence/SAC sequence ........................................................................................177
Serial number ............................................................................................................178
Setup menu ...............................................................................................................178
Simultaneous additions (Yes/No) ...........................................................................179
Skip empty position .................................................................................................179
Software version .......................................................................................................179
Solution menu ...........................................................................................................180
Stability ......................................................................................................................181
Standard (conductivity standard) ...........................................................................182
Standard addition ID ................................................................................................183
Standard addition method .......................................................................................183
Standard potential ....................................................................................................183
Standard solution (conductivity measurements) ..................................................183
Standard solution (ISE measurements) .................................................................183
Statistics ....................................................................................................................183
Std add volume Automatic/Programmed/ ..............................................................184
Std conc. too low (or high) ......................................................................................184
Stirring .......................................................................................................................185
Stop analysis ............................................................................................................186
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Supervisor code .......................................................................................................187
Supervisor mode ......................................................................................................188
T°C minimum/maximum value ................................................................................188
Target titre .................................................................................................................188
Temp. coef. ................................................................................................................188
Temp. correction None/Linear/Nat. water ..............................................................189
Temp. limit exceeded ...............................................................................................190
Temperature Probe/ Fixed at 25°C/Entered ............................................................190
Temperature sensor ID ............................................................................................191
Test amount ..............................................................................................................191
The sequence is empty ............................................................................................191
Time max (result indicator) ......................................................................................191
Title ............................................................................................................................191
TTL 5 V OUT/TTL 12 V OUT (sockets) .....................................................................192
TTL IN (sockets) .......................................................................................................192
Type of method .........................................................................................................192
User ID (Yes/No) .......................................................................................................193
User list .....................................................................................................................193
User’s rights ..............................................................................................................194
Valency ......................................................................................................................195
Volume .......................................................................................................................195
Vstd>max (result indicator) .....................................................................................195
Working mode ...........................................................................................................196
Wrong buffer .............................................................................................................196
Zero pH ......................................................................................................................196
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Appendixes ........................................................................................................197
Appendix 1: Preprogrammed methods ..................................................................199
Appendix 2: General information ............................................................................201
Appendix 3: Result calculations .............................................................................203
Appendix 4: Technical specifications ....................................................................217
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ION570 Reference Manual
Introduction
The ION570 Ion Analyser is dedicated for routine use. It offers two distinct user levels:
• Supervisor
Dedicated for operators who wish to edit their methods to fit their specific needs. They can
also assign a password to protect the programmed data from eventual changes.
•Routine
Dedicated for operators wishing to use the routine functions to guide them step by step
through the analyses.
The ION570 can store up to 50 methods, 30 electrodes and 30 reagents. In addition 30
electrodes and 20 reagents have been pre-defined to help you save time setting up your
application.
Thanks to the preprogrammed applications, the Ion Analyser is ready for use as soon as it has
been installed. Refer to "Appendix 1: Preprogrammed methods", page 199.
The ION570 also allows you to automatically sequence and repeat measurements.
The purpose of the ION570 Reference Manual is to give detailed information on the
Ion Analyser and the data displayed during operations. The information is listed in
alphabetical order for quick access and cross-references are listed in italics.
In addition to this handbook, a general User’s Guide (part no. D21M069) is available giving
descriptions and overviews of the workstation menus and operating concepts to guide you
through programming and running of the analyses.
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Read me!
An important feature of this instrument interface is that it controls the presence of different
elements necessary to run the defined application for a selected method/sequence, before the
method/sequence is run.
Working in Supervisor mode
A Supervisor has access to all the libraries for creation purposes.
When programming the instrument in “SUPERVISOR” mode, it is recommended to work in
stages. These stages must be carried out in the order described below:
A.To program method
1. Define your electrode(s)
Identify electrodes (including temperature sensors) to be used for the analysis:
Electrodes can be created from the following lists:
Catalogue, see "Catalogue list", page 62.
Other, see "Others list", page 139.
Copy from, see "Copy electrode", page 73.
When creating the electrode, define if electrode calibration is required (or not), if yes specify
the "periodicity" of the calibrations and the pH, ISE or conductivity standards to be used.
Refer to "Calibrate pH electrodes", page 55.
Refer to "Calibrate ISE electrodes", page 55.
Refer to "Calibrate conductivity cells", page 55.
2. Define reagent
Identify reagents to be used for the analysis
Reagents can be created from the following lists:
Catalogue, see "Catalogue list", page 62.
Other, see "Others list", page 139.
Copy from, see "Copy reagent", page 74.
If a sample changer is to be used, define the sample changer in the Configuration menu before
selecting a SAC sequence.
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3. Create new method or Edit a pre-programmed one
Create the method to be used for the analyses. Enter the parameters required to calculate the
results, see "Programming methods", page 27.
When you have finished programming, select the method/sequence or pre-programmed
application, see "Select method", page 175 or see "Select sequence", page 176.
If your methods are to be performed in a sequence, program the sample stack, see "Sample
stack", page 174.
4. Check icons
The following icons indicate the exact state of your working system:
Sunny icon:
Everything is OK. Run the method or sequence.
Cloudy icon:
Action required within 12 or 24 hours (for a calibration) and 1 week (for
a reagent replacement).
Stormy icon:
Electrode calibration date elapsed or electrode(s)/reagent(s) not
installed.
Question mark:
Programming error.
Refer to "Electrode icons", page 104.
Refer to "Reagent icons", page 153.
Sunny icons are needed in order to run the selected method.
If Cloudy/Stormy/Question mark icons are displayed in the Reagent/Electrode window
press 1 to activate the “Check” command. The ION570 will automatically guide you
through the operations required to solve the errors encountered.
B.Running methods
To run a method or sequence, see "Working in Routine mode", page 16.
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Working in Routine mode
A.Access methods
A Routine operator has access to all the methods “Select method” and programmed
parameters “Display method” for checking purposes
B.Running methods
When working in “ROUTINE” mode, it is necessary to install your measurement system
according to the selected method or sequence, prior to running a method or sequence.
1. Select the method or sequence
Refer to "Select method", page 175.
Refer to "Select sequence", page 176.
2. Check icons
Refer to "Check icons", page 15.
Depending on the icons displayed, the ION570 will automatically guide you through the steps
necessary to run the analysis, see below:
a. Connect the electrode(s)
Connect/install electrodes and temperature sensors, Refer to "Electrode connection",
page 102.
b. Install regents(s)
Check that the burette is installed, if not, see "Install burette", page 119.Now,install
the reagent, see "Install reagent", page 120.
c. Calibrate electrode(s)
Now, run the calibration.
Refer to "Calibrate pH electrodes", page 55.
Refer to "Calibrate ISE electrodes", page 55.
Refer to "Calibrate conductivity cells", page 55.
d. Enter reagent titre (only for an ISE standard addition method)
Now, enter the reagent titre.
Refer to "Enter titre", page 108.
e. Run the method or the sequence
Refer to "Running a method/sequence", page 170.
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Practical examples
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Programming electrodes
pH electrodes
ION570 Reference Manual
1.
Press 4.
4.
2.
Press 1.
5.
3.
Select function and ID.
6.
Select ID from Catalogue or
Others list.
Press 1 to confirm.
Press 1 to confirm the creation
of the new electrode.
For a combined or a simple or
reference electrode, enter the
potential (in mV) of the reference versus the Standard Hydrogen Electrode (SHE).
For a combined or a simple
electrode if you have selected
the Others list, enter the internal pH of the electrode.
Enter the electrode address.
If you want a message to be
displayed once a week concerning this electrode, select
Maintenance = Yes and enter
the message.
Select Fixed or Free if a calibration is required, go to step 7.
Select No, for no calibration,
press Esc to leave the menu.
Programming is completed.
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Calibration request = Fixed
Calibration with automatic recognition of the buffer among a list of predefined values. The buffer
values are entered during method edition.
Calibration request = Free
The buffer values are entered FREEly by the user. Use this option to calibrate pH electrode with
buffers that do not belong to the predefined list.
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7.
Enter the calibration
parameters.
10.
8.
Press 1.
11.
9.
Enter the electrode calibration
parameters.
For a Fixed calibration, press
Esc then 2. Go to step 10.
For a Free calibration, press
Esc then 3. Skip to step 11.
12.
Fixed calibration only.
Select the buffer solutions
used. Press Esc then 3.
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Enter the results parameters.
Press Esc then 4.
Enter the printouts parameters.
Press Esc twice. Electrode
programming is completed.
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ISE electrodes
ION570 Reference Manual
1.
Press 4.
4.
2.
Press 1.
5.
3.
Select function and ID.
6.
Select ID from Catalogue or
Others list.
Press 1 to confirm.
Press 1 to confirm the creation
of the new electrode.
For a combined or a simple or
reference electrode, enter the
potential (in mV) of the
reference versus the Standard
Hydrogen Electrode (SHE).
IfyouhaveselectedtheOthers
list, select the valency and
enter the molar weight of the
ion under study.
Enter the electrode address.
If you want a message to be
displayed once a week
concerning this electrode,
select Maintenance = Yes and
enter the message.
Press the Left or Down arrow
key.
Select Manual or Automatic if a
calibration is required then go
to step 7.
Select No, for no calibration,
press Esc to leave the menu.
Programming is completed.
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Calibration = Manual
Calibration using 1 to 9 standards of known concentration.The standard concentrations are entered
during method edition. This method requires 1 to 9 calibration beakers.
Calibration = Automatic
1 to 4-point calibration method where the standards are automatically prepared by the Ion Analyser
using an automatic addition method. The standard concentrations and an intial volume of standard
are entered during method edition. This simple to use method requires only one calibration beaker.
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ION570 Reference Manual
7.
Enter the calibration
parameters.
10.
8.
Screen obtained in the case of
a Manual calibration.
Press 1.
11.
9.
Screen obtained in the case of
a Manual calibration.
Enter the electrode calibration
parameters.
Press Esc then 2.
12.
Screen obtained in the case of
a Manual calibration.
Enter the standard solution ID
and standard concentrations.
Press Esc then 3.
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Enter the results parameters.
Press Esc then 4.
Enter the printouts parameters.
Press Esc twice. Electrode
programming is completed.
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Conductivity cells
ION570 Reference Manual
1.
Press 4.
4.
2.
Press 1.
5.
3.
Select function and ID.
6.
Select ID from Catalogue or
Others list.
Press 1 to confirm.
Calibration request = Free
Use the Free calibration mode when you use a standard that does not belong to the Catalogue list
and you know the conductance of this standard at a given temperature. During a Free calibration run
and after stabilisation of the measurement, you will adjust the cell constant in order to display the
correct conductance value.
Calibration request = Fixed
With the Fixed mode when you use a standard that belongs to the Catalogue list, the cell constant is
determined as the ratio of the conductivity (known by the instrument) divided by the measured
conductance.
Press 1 to confirm the creation
of the new electrode.
IfyouhaveselectedtheOthers
list, enter the cable resistance
and capacitance.
If you want a message to be
displayed once a week concerning this electrode, select
Maintenance = Yes and enter
the message.
Select Fixed or Free if a
calibration is required, go to
step 7.
Select No, for no calibration,
press Esc to leave the menu.
Programming is completed.
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7.
Enter the calibration
parameters.
10.
8.
Press 1.
11.
9.
Enter the electrode calibration
parameters.
For a Fixed calibration, press
Esc then 2. Go to step 10.
For a Free calibration, press
Esc then 3. Skip to step 11.
12.
Fixed calibration only.
Select the standard solution
used. Press Esc then 3.
Enter the results parameters.
Press Esc then 4.
Enter the printouts parameters.
Press Esc twice. Electrode
programming is completed.
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Programming reagents
ION570 Reference Manual
1.
Press 4.
4.
2.
Press 1.
5.
3.
Press ✓ .
6.
Select ID from Catalogue list or Others list.
Enter the target titre and unit.
Press 1 to confirm.
Confirm the creation of the new
reagent.
Enter the burette address.
Press Esc twice to leave the
menu.
Programming is completed.
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Programming methods
Creating and editing a method
ION570 Reference Manual
1.
Press 4.
4.
2.
Press 1.
5.
3.
Enter ID.
Press 1 to confirm.
6.
Enter method parameters.
Specify the Mode, see "Mode",
page 135.
Press 1.
Press ✓ and select the
electrode(s) and temperature
sensor from the lists.
Enter the other method
parameters.
For an ISE standard addition
method (as shown in our
example), press Esc then 2
and go to the next step.
For all other methods, press
Esc then 3 and skip to step 8.
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7.
Enter the Sample parameters.
Press Esc then 3.
10.
8.
Enter the results parameters.
Press Esc then 4.
9.
Enter the printouts parameters.
If a QC sample has been
defined in step 4, press Esc
then 5.
Enter the QC data. Press Esc
twice. Method programming is
completed.
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For a Coupled method
ION570 Reference Manual
1.
Press 4.
4.
2
Press 1.
3.
Enter the Method ID and press
1 to confirm.
Select Mode = Coupled.
Enter the method to be linked.
Press Esc twice.
Method programming is
completed.
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ION570 Reference Manual
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Programming sequences
ION570 Reference Manual
1.
Select:
Sequence:tocreatea
sequences of methods.
SAC Sequence:tocreatea
sequences of methods to be
performed using a Sample
Changer.
Define the Sample
Changer in the
Configuration menu
before selecting SAC
Method or SAC
Sequence.
2
Press 2.
3.
Enter a name for the sequence.
4.
Press 3.
5.
Press 1 to add a method.
6.
Select the type of method.
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7.
Selectamethodinthelistof
available methods.
10.
8.
Press 1 to add the method to
the sequence.
9.
If Sample has been selected in
step 6, enter the number of
samples (number of times you
wish to repeat the method in
the sequence).
Press 1 to add a second
method to the sequence.
Repeat steps 6 to 9.
Up to 10 methods can be
chained in a sequence.
After having added the last
method, press Esc twice.
Sequence programming is
completed.
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Programming tips
• Do not forget to declare electrode(s) and reagents(s) when programming your method
parameters
• If a Sample Changer is used, do not forget to declare one in the Configuration menu.
• If a printer is used, do not forget to declare one in the Configuration menu.
If no sun icons appear after the method has been selected, check the following points:
1. Install electrode(s) for selected method, see "Check electrodes", page 66.
2. Install reagent(s) for selected method, see "Install reagent", page 120.
3. If required, calibrate electrode.
Refer to "Calibrate pH electrodes", page 55.
Refer to "Calibrate ISE electrodes", page 55.
Refer to "Calibrate conductivity cells", page 55.
4. For an ISE standard addition method only, enter standard reagent titre, see "Enter titre",
page 108.
If the Sunny icons appears:
Everything is OK. A sunny icon is required to run the selected method.
If the Cloudy icon appears:
An electrode calibration should be performed within 24 hours. The expiry date of
one of the reagents in the system will expire in less than one week.
This is a simple warning, it will not stop you from running the analysis.
If the Stormy icon appears:
Reagent and/or electrode required in the selected method is/are not installed.
Electrode required in the selected method has not been calibrated.
If the Question mark icon appears:
It is a programming error, reagent and/or electrode is/are not defined in the
selected method. Revise the method programming.
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When a Stormy or a Question mark icon appears, press 1 “Check” . The ION570
will automatically guide you through the operations necessary to solve the errors
encountered.
ION570 Reference Manual
Page 34
Glossary
MeterLab
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ION570 Reference Manual
ABU1/ABU2
ABU1 means an ABU52 connected to the Local socket of the ION570.
ABU2 means an ABU52 connected to the Slave socket of a second
ABU52 itself connected to the Local socket of the ION570.
ION570
Accept a result
Figure 1: ABU1 and ABU2
ABU1 and ABU2 identify the ABU52 used for electrode connections
and burette installations, see "Address", page 44.
Two ABU52 Biburettes can be connected to one ION570.
Refer to "Result accepted (Yes/No)", page 161.
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Acceptance criteria
Acceptance criteria = Yes
Enables the user to enter preset minimum and maximum values for
measurement results. If the result lies outside these values an alarm
message appears and the results are rejected by the instrument. The
Supervisor is the only person allowed to accept a result that has been
rejected by the instrument, see "Result accepted (Yes/No)", page
161.
Therefore, acceptance limits can be set on:
• the conductivity cell constant,
see "Min. cell cst - Max. cell cst", page 133.
• the result value such as a pH, a potential, a concentration,
see "Minimum value - Maximum value", page 135.
• the response slope of a pH or an ISE electrode,
see "Min. sensitivity - Max. sensitivity", page 134.
• the pH0 of a pH electrode,
see "Min. pH0(25) - Max. pH0(25)", page 134.
Acceptance criteria = No
The Supervisor or Routine user is free to accept/reject the results.
Enter in:
Edit method > Results
Edit method > QC data
Edit electrode > Results
Irrespective of the Yes or No option selected for the
Acceptance criteria parameter:
•Acceptance limits must be set for the sample or the
standard measured temperature,
see "Min. Temp. - Max. Temp.", page 134,
see "T°C minimum/maximum value", page 188.
•A minimum limit is set by the instrument for the concentration measured by an ISE Direct measurement method. This
limit is the C
see "Minimum value - Maximum value", page 135.
•A maximum limit is set by the instrument for the concentration measured by an ISE Direct or Standard addition
method. This limit is set to 10
see "Minimum value - Maximum value", page 135.
concentration,
0
30
,
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Acceptation
Access routine mode
Result acceptance time limit.
When the time entered for the Acceptation has elapsed the
measurement will be accepted whether stable or not.
For the signal to be accepted once the Acceptation has
elapsed, the Max. Stab. time must be greater than the
Acceptation time.
Enter in:
Edit method > Parameters menu
Edit electrode > Calibration parameters menu
Range available:
0 to 59:59 min:s
Press Stop for 3 seconds from the Main window then press 2.
These rules can be set by the Supervisor to allow the routine user
access to certain operations.
Active electrode unknown in "method ID"
Enter in:
Setup menu > Access routine mode
The method in use, has at least one electrode which has not been
defined. Press ✓ and declare the electrode in the Electrode ID field of
the Method parameters screen.
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Add method menu
Addition
Use this menu to set the ID and type of method to be added to a
sequence.
In the title bar, x/y (eg. 1/1) indicates the position "x" of the method in
the sequence and "y" the total number of methods in the sequence.
When a sequence is created <1/1> is displayed.
To access:
Press 1 in the Edit sequence menu.
Select Yes to carry out a reagent addition at the start of an ISE
standard addition method.
The reagent is added using a second burette controlled by the ION570.
This second burette is installed on an ABU52 connected to the
ION570, see "ABU1/ABU2", page 37.
Edit an Addition method when you want to perform 2 or 3
reagent additions successively or simultaneously.
Refer to "Addition method - definition", page 42.
Enter in:
Edit method > Parameters
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Addition delay
Parameter of an Addition method, see "Addition method - definition",
page 42.
For a 1-addition method or a multi-addition method with the
Simultaneous additions = Yes option selected, the
Addition delay is counted down at the end of the reagent
addition(s).
For a multi-addition method with the Simultaneous
additions = No option selected, an Addition delay is
to be set for each addition. An Addition delay is counted
down at the end of an addition and before the next addition is initiated.
While an addition method is running, you can carry on
regardless this delay by pressing key Del.
Enter in:
Edit method (Addition method).
Range available:
00:00 to 99:59 min:s
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Addition
method definition
An Addition method performs 1 to 3 reagent additions. These
additions can be runned simultaneously or one after the other with or
without a delay between 2 additions. No measurements are
performed. An example of use is to place an Addition method in a
Coupled method before a Measurement method.
Programming an Addition method
1. In the Edit method menu, select Mode = Addition.
2. On the next line, enter the number of additions to be done (1 to 3).
For a 1-addition method
Select the addition reagent name.
Enter the reagent volume to be added (1 µl
to 999 ml)
Enter the delay to wait at the end of the
addition (00:00 to 99:59 min:s).
For a 2 or 3-addition method
Refer to "Addition method - programmation", page 43.
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Addition
method programmation
For a 1-addition method
Refer to "Addition method - definition", page 42.
For a 2 or 3-addition method
At the Simultaneous additions, select whether you want
to perform the additions simultaneously or one after the other.
• In the case of simultaneous additions:
Enter the delay to wait at the end of the 2 or 3 additions (00:00 to 99:59 min:s).
Enter for each addition, the reagent name and the volume to be added (1 µl to
999 ml).
• In the case of additions performed one after the other:
Enter for each addition, the reagent name, the volume of reagent to be added
(1 µl to 999 ml) and the delay to wait at the end of the addition (00:00 to
99:59 min:s).
For the Auxiliary output parameter, see "Auxiliary output", page 50.
In an Addition method, it is not possible to run 2 additions of
the same reagent.
In an ISE standard addition method, it is also possible to run
one reagent addition before the measurement starts,
see "Addition", page 40.
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Addition volume
Address
Refer to "Reagent addition volume", page 153.
The position where the electrode and burette are placed during
operation:
Electrode
The electrode address is defined using the format “xxx/y” where “xxx”
corresponds to the instrument (ION, ABU1 or ABU2) where the
electrode is connected and “y” corresponds to the socket.
For example ION/E1, indicates that the electrode is connected to E1
socket on the ION570.
It is always recommended to connect an indicating electrode
and its associated reference to the same instrument (ION,
ABU1 or ABU2).
Refer to "Electrode connection", page 102.
Burette
The burette address is defined using the format “xxx/y” where “xxx”
corresponds to the instrument (ION, ABU1 or ABU2) where the burette
is placed and “y” corresponds to the position. For example ION/1
indicates that the burette is placed on the ION570 in position 1.
Alarm: Locked
Position 2
Figure 2: Burette positions
Refer to "ABU1/ABU2", page 37.
The user cannot bypass an electrode and/or QC sample analysis if the
last result obtained lies outside the acceptance range.
Enter in:
Setup menu > Access routine mode
Position 1
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Alarm: Unlocked
Aliquot
Enables the user to bypass an electrode and/or QC sample analysis
when the last result obtained lies outside the acceptance range.
Enter in:
Setup menu > Access routine mode
Amount that is taken from the diluted sample. This amount is used for
analysis.
y ml (or g) of sample + solvent
The quantity of species present in the sample is calculated
1.
Sample amount
2.
Dilution under stirring
with a solvent to obtain
a final amount of x ml (or g)
3.
Analysis
using the result obtained at the end of the analysis.
Quantity of species in sample = Result
x = Final dil. amount
y = Aliquot
x
x
y
Figure 3: What is an aliquot?
Enter in:
Edit method > Sample
Range available:
0.001 to 100000 (unit = Sample unit)
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Alphanumeric characters
The following alphanumeric characters can be obtained using the
ION570s Keypad:
Keys Characters
7 7,A,B,C,a,b,c,@
8 8,D,E,F,d,e,f
9 9,G,H,I,g,h,i
4 4,J,K,L,j,k,l
5 5,M,N,O,m,n,o,µ
6 6,P,Q,R,p,q,r
1 1,S,T,U,s,t,u
2 2,V,W,v,w
3 3,X,Y,Z,x,y,z
0 0,-,+,*,^,=,#,<,>,.
space, /, (, ), [, ], |, ?, !, %, °
Applied signal (AC/DC)
Archives data lost - Cal. Data lost - Methods kept
Table 1: Entering alphanumeric characters
Specifies the current type (alternative AC or direct DC) to be sent to
the Pt-Pt socket on the Ion Analyser. The AC signal frequency is
1.67 Hz. This option is available if mV(i>0) has been selected for
Measurement in the Edit method menu.
Enter in:
Method parameters menu
Instrument internal failure. Only the method parameters have been
kept.
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Archiving
Assistant function
Archiving = Yes (default setting)
All measurements (sample, electrode calibrations and reagent titre entries) are saved in the archives. You can view these measurements as
follows:
• Sample results: enter Main window and press 5
• Reagent titre entries: enter Reagent window and press 6
• Electrode calibration results: enter Electrode window and press 6
Refer to "GLP-Archives menu", page 115.
Archiving = No
No measurements are saved in the archives. The instrument saves
only the last electrode calibration.
When you set Archiving from No to Yes, you must recali-
brate your electrodes and re-enter your reagent titre!
Enter in:
Setup menu > Configuration menu
Embedded instructions on the ION570 display to guide the user stepby-step through electrode and reagent installations. These instructions
appear at the start of a run method if the working system has not been
correctly installed.
Automatic (calibration mode)
By default this option is set to Yes. It is recommended to use
the default setting at all times!
If the setting is set to No, the ION570 considers that the working
system is correctly installed at the start of a run method.
However, this may not be the case, the user must know the status of
the working system at all times!
Enter in:
Setup menu > Configuration menu
Refer to "Electrode calibration (ISE)", page 96.
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Autochaining
This option is valid for a Coupled method which is not part of a
sequence.
Autochaining = No
At the end of each method run, you must confirm the result in order to
perform the next method. If a Notification message has been selected,
a message is displayed between each method of the Coupled
method.
Autochaining = Yes
At the end of each method run, The methods are chained
automatically in the Coupled method. If a Notification message has
been selected, a message is displayed upon starting the first method
(no message is displayed after).
Refer to "Notification message", page 136.
Enter in:
Edit method menu (for a Coupled method)
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Auxiliary input
The auxiliary input socket can be connected to an external device unit
used to send an analysis start command to the ION570. The analysis
is a sequence of methods with manual change of the sample beakers
(Working mode = Sequence, see "Working mode", page 196).
The external device unit is to be connected to the red and black IN
banana sockets of the ION570. The red banana socket receives the
TTL 0 ± 5 V auxiliary signal and the black banana socket is connected
to the instrument electrical zero.
Proceed as follows to trigger a sequence of methods by an auxiliary
signal input:
• In the Configuration menu, select
Controlled by TTL IN = Yes.
• Connect the auxiliary control unit to red and black IN banana
sockets of the ION570.
• Run the sequence. The ION570 displays a waiting for auxiliary
signal message. The sequence is started as soon as the auxiliary
signal is received.
Spécifications of the auxiliary input signal
Refer to "TTL IN (sockets)", page 192.
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Auxiliary output
The auxiliary outputs are used to control external equipment such as
valves or pumps during analyses. This signal is sent between the red
and black banana sockets 5V OUT or 12V OUT of the ION570.
Auxiliary output (5 V, 12 V, No)
Activate to 5 V or 12 V or disable the auxiliary signal.
Specifications of the auxiliary ouput signal:
see "TTL 5 V OUT/TTL 12 V OUT (sockets)", page 192.
Aux. on for
Time during which the auxiliary signal is set to 5 V or 12 V.
Enter in:
Method parameters menu
Range available:
Aux. on for: 0 to 99:59 min:s
An auxiliary output can be activated:
•at the measurement start or before the first reagent addition
(duration set by Aux. on for)
Aux. on for
Bar code reader connection
Batch number
•or during the whole measurement including measurement
stabilisation delay or during all the reagent addition(s). In this
case, select a 5 V or 12 V auxiliary output and set
Aux. on for = 0.
Refer to "Auxiliary output", page 50.
Connect a bar code reader to the ION570 via the 6-pin mini DIN port
situated on the right hand side of the instrument.
Up to 16 alphanumeric characters can be entered when installing or
replacing a reagent. It is the reagent identification number given on
the reagent bottle.
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Beaker menu
Use this menu to prepare a sample or calibration stack. This menu
defines individual data for all the samples or standards used in the
sequence.
Figure 4: Beaker menu (for a sample stack)
To access (for a sample stack):
1. Select Working mode = Sequence or SAC Sequence in the Main
window.
2. Press 2 Sequence/Sample stack.
3. Press 1 Sample stack.
Beakers: [F;L]
The sequence must have been edited in the Edit sequence
menu beforehand. Refer to "Edit sequence menu", page 93.
To access (for an electrode calibration stack):
1. Select Working mode = SAC Sequence in the Main window.
2. In the Electrode window, press 1 Calibrate electrodes.
3. Press 2 Calibration sequence.
The electrode calibration method must have been edited
beforehand. Refer to "Edit electrode menu", page 90.
Refer to "Sample stack", page 174.
Refer to "Electrode calibration stack", page 101.
The beakers information is displayed in the Edit sequence menu of a
sequence.
It indicates the First and Last positions occupied by the beakers in the
sequence.
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Beep
Burette functions menu
If Yes has been selected, three beeps will sound when a result is
obtained.
Enter in:
Setup menu > Configuration
These functions can be activated during the preparation of the
burette, before installing the reagent.
To access, press 7 in the Reagents window. To run a burette function,
select the burette in the Address field, then press 1 to 6 corresponding to the required procedure. The title bar shows the volume of
the burette in use. You can replace (key 5) or remove this burette
(key 6).
Burette speed
Burette volume
C0(Detection limit)
Figure 5: Burette functions menu (burette installed/not installed).
The maximum burette speed (in ml/min) is three times the nominal
value of the burette per minute. However, for the 50 ml burette the
maximum burette speed is 1.5 times the nominal value, i.e. 75 ml/min.
The burette volume is entered while installing or replacing the burette.
This volume is indicated on the burette.
Enter in:
Install burette or Replace burette menu.
Range available:
1ml,5ml,10ml,25mlor50ml.
Refer to "Direct ISE measurement method - definition", page 83.
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Cable capacity
A cable of a given length has a given capacity. When the measured
conductance is low (below 4 µS), the cable capacity is not
negligible and must be taken into account.
Enter the cable capacity when:
• measuring low conductances (below 4 µS),
• the cable capacity of the conductivity cell is greater than 350 pF.
The cable capacity is normally specified by the manufacturer. Cable
capacities of a few Radiometer Analytical conductivity cells are given
below :
Conductivity cell Cable capacity (pF)
CDC511T 500
CDC861T 500
CDC565 440
CDC749 170
CDC267-9 with cable A94L136 70
CDC267-9 with cable A94L336 200
CDC241-9 with cable A94L136 70
CDC241-9 with cable A94L336 200
XE100 with cable A94L136 70
XE100 with cable A94L336 200
Figure 6: Cable capacities of Radiometer Analytical conductivity cells
If you create a conductivity cell from the Catalogue list, the
cable capacity is automatically assigned to the
conductivity cell created (and cannot be modified).
Enter in:
When creating an electrode with the Conductivity function and the
option From = Other.
Refer to "Create electrode", page 76.
Available limits:
0 to 1999 pF
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Cable resistance
A cable has a given length, therefore a given resistance. When the
measured resistance is low (below 50 Ω), the cable resistance is not
negligible and must be taken into account.
Enter the cable resistance when:
• measuring low resistances (below 50 Ω) or high conductances
(above 20 mS).
• using a 2 or 3-pole conductivity cell.
The cable resistance is normally specified by the manufacturer.
Cable resistances of a few Radiometer Analytical conductivity cells
are given below:
Conductiivity cell Cableresistance(Ω)
CDC511T 0
CDC861T 0
CDC565 0
CDC749 0.180
CDC267-9 with cable A94L136 0.145
CDC267-9 with cable A94L336 0.350
CDC241-9 with cable A94L136 0.145
CDC241-9 with cable A94L336 0.350
XE100 with cable A94L136 0.145
XE100 with cable A94L336 0.350
Figure 7: Cable resistances of Radiometer Analytical conductivity cells
If you create a conductivity cell from the Catalogue list, the
cable resistance is automatically assigned to the
conductivity cell created (and cannot be modified).
Enter 0 for the cable resistance of a 4-pole conductivity cell
(whatever the conductivity cell used).
Enter in:
When creating an electrode with the Conductivity function and the
option From = Other.
Refer to "Create electrode", page 76.
Page 54
Available limits:
0.000 to 9.999 Ω
MeterLab
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ION570 Reference Manual
Calibrate conductivity cells
Calibrate ISE electrodes
Calibrate pH electrodes
Refer to "Electrode calibration (Fixed mode, conductivity cell)", page
94.
Refer to "Electrode calibration (Free mode, conductivity cell)", page
95.
Refer to "Electrode calibration (ISE)", page 96.
Refer to "Electrode calibration (Fixed mode, pH electrode)", page 97.
Refer to "Electrode calibration (Free mode, pH electrode)", page 98.
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Calibration = Automatic
Available if Electrode type = ISE single, ISE combined (w/o temperature sensor),
This ISE electrode calibration mode is simple to use as the user has
just to pour into a beaker a known amount of supporting electrolyte.
The 1 to 4 standards needed for the calibration are prepared by the
instrument by automatic additions. The user enters, in the electrode
calibration parameters, the initial volume of electrolyte solution and
each standard concentration.
What you
have to do
In Edit mode,
enter:
Initial volume
and
Concentration n
(n=1 to 4 with
C1 < C2 < C3
< C4˚)
Electrolyte
solution =
Initial volume
Addition of
standard
x1 ml
Standard no.1 =
Initial volume + x1 ml
Standard no.2 =
Initial volume + x1+x2 ml
Standard no.3 =
Initial volume + x1+x2 + x3 ml
What is done automatically by the instrument
Measurement
Addition of standard
x2 ml
Measurement
Addition of standard
x3 ml
Measurement
Calibration
point no.1
E1, Concentration 1
Calibration
point no.2
E2, Concentration 2
Calibration
point no.3
E3, Concentration 3
Calibration
curve
Calibration
results
Page 56
Addition of standard
x4 ml
Measurement
Standard no.4 =
Initial volume + x1+x2 + x3 + x4 ml
4 standards can be prepared
Calibration
point no.4
E4, Concentration 4
Figure 8: ISE electrode calibration in Automatic mode
See also: "Calibration = Manual": see page 57.
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ION570 Reference Manual
Calibration = Manual
Available if Electrode type = ISE single, ISE combined (w/o temperature sensor),
In this ISE electrode calibration mode, 1 to 9 standard of known
concentration are to be prepared. The user enters each standard
concentration in the Edit electrode > Solution menu.
What you have to do What is done automatically by the instrument
In Edit mode,
enter:
Concentration n
(n=1 to 9)
Measurement
Standard no.1 =
Concentration 1
Measurement
Standard no.2 =
Concentration 2
Calibration
point no.1
E1, Concentration 1
Calibration
point no.2
E2, Concentration 2
Calibration
curve
Calibration
results
Measurement
Standard no.3 =
Concentration 3
9 standards
can be prepared
Calibration
point no.3
E3, Concentration 3
Figure 9: ISE electrode calibration in Manual mode
See also: "Calibration = Automatic": see page 56.
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Calibration curve of an ISE electrode
Calibration delay elapsed
Calibration parameters
This is the E = f (pC = -log C) curve obtained at the end of a
calibration cycle performed on an ISE electrode.
Displaying the calibration curve:
Refer to "Electrode calibration (ISE)", page 96.
Printing the calibration curve:
The curve is printed out automatically at the end of each calibration
cycle if asked for in the Printouts menu of the calibration method,
see "Printouts setup", page 148.
This message appears at analysis start. A new electrode calibration is
required. The delay Periodicity entered in the Edit electrode screen
has elapsed, see "Periodicity", page 141.
Press ✓ and perform a calibration.
For an electrode calibration method, see "Electrode calibration
parameters", page 100.
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Calibration point menu
This menu is available for ISE electrode calibration that is edited with
the Calibration = Automatic option.
These parameters describe how the standards are prepared using the
automatic addition method.
With the automatic addition method, one beaker is only necessary to
prepare for all the standards (1 to 4 standards).
An initial volume of electrolyte support solution is poured into the
beaker. The user enters this initial volume in the Calibration parameters ( see "Initial volume", page 118)andthe1to4standard
concentrations in the Calibration point menu.
The Ion Analyser calculates the amounts of standard to be added to
reach these concentrations then performs the necessary additions.
To access:
1. From the Electrode window, press 4.
2. Select the ISE electrode to be edited.
3. Press 2 Edit electrode and check that the Calibration =
Automatic option has been selected.
4. Use the LEFT/RIGHT arrow keys to move to the last
Edit electrode display.
5. Press 2 Calibration points.
Select the standard used from the User or the
Catalogue list.
Select the standard concentration unit.
Available units: eq/l, meq/l, mol/l, mmol/l, g/l,
mg/l, mg/ml, µg/ml, % or ppm.
Enter the concentration of each standard with
the unit selected above.
Available limits: 10
-10
to 1010.
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Calibration request/ Calibration
Available if Electrode type =
• pH single, pH combined (w/o temperature sensor),
• ISE single, ISE combined (w/o temperature sensor),
• Conductivity (w/o temperature sensor).
Select the option Calibration request = Fixed or
Free to calibrate a pH electrode or a conductivity cell.
Select the option Calibration = Manual or Automatic
to calibrate an ISE electrode.
The corresponding calibration parameters and standards will be
displayed.
Enter in:
Edit electrode menu
Refer to "Calibration = Automatic", page 56.
Refer to "Calibration request = Fixed", page 61.
Refer to "Calibration request = Free", page 62.
Refer to "Calibration = Manual", page 57.
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Calibration request = Fixed
Available if Electrode type = pH single, pH combined (w/o temperature
sensor), conductivity (w/o temperature sensor).
In this calibration mode, the electrode is calibrated with standards that
belong to a list of predefined values.
Moreover, for a pH electrode, the buffers/standards are automatically
recognised.
The user selects the buffer/standard values during method edition.
Use this mode if you intend to calibrate the electrode using buffers/
standards of the ION570 predefined list.
pH Buffer
(value at 25°C)
IUPAC - 1.679 pH S11M001 (500 ml)
IUPAC - 4.005 pH S11M002 (500 ml)
IUPAC - 6.865 pH S11M003 (500 ml)
IUPAC - 7.000 pH S11M004 (500 ml)
IUPAC - 7.413 pH S11M005 (500 ml)
IUPAC - 9.180 pH S11M006 (500 ml)
Radiometer Analytical part no.
IUPAC - 10.012 pH S11M007 (500 ml)
IUPAC - 12.454 pH S11M008 (500 ml)
pH 4 S11M012 (500 ml)
pH 7 S11M013 (500 ml)
pH 10 S11M014 (500 ml)
Table 2: pH buffers of the ION570 predefined list
Conductivity standard Radiometer Analytical part no.
1 D KCl S51M001 (500 ml)
0.1 D KCl S51M002 (500 ml)
0.01 D KCl S51M003 (500 ml)
0.1 M KCl C20C250 (500 ml)
0.01 M KCl C20C270 (500 ml)
0.001 M KCl C20C280 (500 ml)
0.05 % NaCl S51M004 (500 ml)
25 µS/cm NaCl S51M013 (250 ml)
Table 3: Conductivity standards of the ION570 predefined list
See also: "Calibration request = Free": see page 62.
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Calibration request = Free
Calibration stack
Calibration results parameters
Available if Electrode type = pH single, pH combined (w/o temperature
sensor), conductivity (w/o temperature sensor).
In this calibration mode, the buffer/standard values are entered
FREEly by the user. Use this option to calibrate pH electrode or
conductivity cells with buffers/standards that do not belong to the
instrument predefined list. You must accurately know the
pH/conductivity of the buffer/standard at given temperatures.
When running a calibration in Free mode and after stabilisation of the
measurement, the user enters the pH buffer/standard conductivity value at the temperature measured in the buffer/standard.
See also: "Calibration request = Fixed": see page 61.
For an electrode calibration method, see "Electrode calibration
stack", page 101.
Refer to "Results menu", page 164.
Catalogue list
List of Radiometer Analytical names of electrodes and commonly
used reagents. This list cannot be modified.
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Cell constant (parameter)
Enter the cell constant value. The cell constant is a specification of the
conductivity cell and is normally provided by the cell manufacturer.
If you do not know the cell constant value or if you want to
check its value, select
or
Fixed
It is recommended to periodically check the constant value by
performing a cell calibration.
Refer to "Electrode calibration (Fixed mode, conductivity
cell)", page 94.
Refer to "Electrode calibration (Free mode, conductivity
cell)", page 95.
Refer to "Cell constant (definition)", page 64.
Access:
Edit Electrode menu (for a Conductivity type of electrode with
Calibration request = No)
Free
Calibration request =
, edit and run a calibration method.
Range available:
0.050 to 15.000 cm
-1
(by steps of 0.001 cm-1)
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Cell constant (definition)
Cell grounding
The ION570 calculates and displays the κ conductivity based on a G
measured conductance and the K cell constant of the conductivity cell
used.
-1
.cm
κ (in S
The K constant (expressed in cm
conductivity cell depending on the cell geometry.
To measure conductivities, you must know the cell constant value.
With the ION570, you can directly enter K in the Edit electrode menu (
see "Cell constant (parameter)", page 63)or
determine K by calibrating the conductivity cell
( see "Electrode calibration (Fixed mode, conductivity cell)", page 94
or see "Electrode calibration (Free mode, conductivity cell)", page
95).
Defines the grounding of the measuring cell. Select one of the
following options:
Reference
Grounding is ensured by a reference electrode - general use.
)=KxG(inS)
-1
) is a characteristics of the
Metal
Grounding is ensured by a metal electrode connected to the GND
socket on the ION570. Use this option in case of high
resistive solutions in order to avoid measuring background noise at
the electrodes.
Others
Grounding is not ensured by the reference electrode and is defined
outside the method.
Enter in:
Edit method menu
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Cell window
Change electrode name
Use LEFT/RIGHT arrow keys to access.
This window controls the stirring function of the measurement cell.
Select the instrument having the stirrer
(ION for ION570, ABU1 or ABU2
for an ABU52).
Start/stop stirrer
Select stirring speed: 100 to 1100 rpm
Animated icon: indicates when stirrer
or propeller is in operation
An external stirrer (propeller) can be connected to a ION570 or an
ABU52.
Refer to "Stirring", page 185.
1. Display the Electrode window.
2. Press 4 then 2.
Change method name
Change reagent name
Change sequence name
3. In the ID field, enter the new name for the electrode
(16 characters maximum).
1. Display the Main window.
2. Press 4 then 2.
3. In the ID field, enter the new name (16 characters maximum).
1. Display the Reagent window.
2. Press 4 then 2.
3. In the ID field, enter the new name (20 characters maximum).
1. Select Sequence in the Main window.
2. Press 2.
3. In the Sequence/Sample stack menu, select ID.
4. Enter the new name (16 characters maximum).
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Check command
If a Stormy or a Question mark icon appears in the Reagent/Electrode
windows, press 1 to run the “Check” command. The ION570 will
automatically guide you through the operations required to solve the
problems encountered.
For example:
Press 1
Press ✓
Check electrodes
Page 66
Press 1 to start the Electrode
Installation procedure.
Press 3 in the Electrode window to display the parameters of the
current electrode used in the system. For example, electrode ID and
address.
MeterLab
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ION570 Reference Manual
Check reagents
Conc. 1 too high compared to reagent concentration
Press 3 in the Reagent window to display the parameters of the
current reagent used in the system. For example, reagent ID, expiry
date etc.
When calibrating an ISE electrode in automatic mode, the first
standard concentration (C
) is higher than 1/3 of the addition reagent
1
concentration.
Revise the method programmation. Decrease the value of Concentration 1 in the Calibration points menu, see "Calibration point menu",
page 59 and/or use a more concentrated reagent standard.
Calibration must be restarted.
The first addition volume Va1used to reach the C1concentration is
equal to:
C
1
Va ⋅
=
1
T
V
init
CC
−
1
with:
. C
: concentration of the standard addition reagent
T
: Initial volume of supporting electrode used
. V
init
If C
=1/3CT, using the equation above, Va1=1/2V
1
init
.
It results that you cannot add more than 50¨% of the initial volume .
It is recommended that the first addition stays below 10% of the
initial volume (C
equal or less than 1/11 of CT).
1
Refer to "Electrode calibration (ISE)", page 96.
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Conc. n too high compared to reagent concentration
When calibrating an ISE electrode in automatic mode, the last
standard concentration (C
) is higher than 1/3 of the addition reagent
n
concentration.
You can ignore the message and run the calibration. The ION570 will
use for calculation the n-1 points that gives concentrations below 1/3
of the addition reagent concentration.
You can also revise the method programmation. Decrease the concentrations in the Calibration points menu, see "Calibration point
menu", page 59 and/or use a more concentrated reagent standard.
The cumulated addition volume Vanused to reach the C
n
concentration is equal to:
C
n
Va ⋅
=
n
V
init
CC
−
nT
with:
. C
: concentration of the standard addition reagent
T
: Initial volume of supporting electrode used
. V
init
Conc. not increasing in “method ID“
If C
=1/3CT, using the equation above, Van=1/2V
n
init
.
It results that cumulated volumes of standard added cannot be higher
than 50¨% of the initial volume.
It is recommended that the cumulated addition volumes stay
below 10% of the initial volume (C
equal or less than 1/11 of CT).
n
Refer to "Electrode calibration (ISE)", page 96.
For an ISE electrode calibration method in automatic mode, the
concentrations entered in the Calibration points menu, see "Calibra-
tion point menu", page 59 are not in increasing order.
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Concentration x
Concentration unit
Concentration of the measured species present in the standard no. x
(x=1 to 9). These x standards are used to calibrate an ISE electrode.
Enter in:
Edit electrode > Solution or Calibration points (for an ISE electrode)
Range available:
-10
10
to 1010(unit = Concentration unit)
Refer to "Calibration point menu", page 59.
Refer to "Solution menu", page 180.
Standard concentration unit used for an ISE electrode calibration.
Enter in:
Edit electrode > Solution or Calibration points (for an ISE electrode)
Range available:
eq/l, meq/l, mol/l, mmol/l, g/l, mg/l, mg/ml, µg/ml, % or ppm
Refer to "Calibration point menu", page 59.
Refer to "Solution menu", page 180.
Conductivity cell
Conductivity cell calibration
Refer to "EC socket", page 87.
Refer to "Electrode calibration (Fixed mode, conductivity cell)", page
94.
Refer to "Electrode calibration (Free mode, conductivity cell)", page
95.
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Conductivity measurement method
Measurement method using a conductivity cell connected to the
ION570 EC socket.
You enter the cell constant of the conductivity cell or detemine it by
calibrating the conductivity cell using a standard solution of known
conductivity against temperature.
Refer to "Cell constant (definition)", page 64.
The ION570 measures the G conductance of the sample then
calculates the κ conductivity using the K cell constant and the
following equation :
-1
.cm
κ (in S
The conductivity determined at the sample temperature can be
corrected back to:
• a reference temperature of your choice (enter the reference temperature and a variation coefficient expressed in %/°C),
• 25 °C by using a correction table based on the variations of the
conductivity against temperature for a natural water.
How to define a conductivity measurement method?
1. In the Main window, press 4 then 2 Edit method.
)=KxG(inS)
2. For Mode, select Measurement.
3. For Measurement,selectConductivity.
4. Edit the other parameters of this measurement method.
How to calibrate a conductivity cell?
Refer to "Cell constant (parameter)", page 63.
Refer to "Electrode calibration (Fixed mode, conductivity cell)", page
94.
Refer to "Electrode calibration (Free mode, conductivity cell)", page
95.
How to run a conductivity measurement method?
Refer to "Running a method/sequence", page 170.
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Configuration menu
Connections
Press Stop 3 seconds in the Main window then press 1.
Contains the configuration parameters for the instrument. .
Refer to "Setup menu", page 178.
ABU52: Refer to "ABU1/ABU2", page 37.
Bar code reader: Refer to "Bar code reader connection", page 50.
Electrodes: Refer to "Electrode connection", page 102.
Connect electrodes
PC keyboard: Refer to "Keyboard connection", page 125.
PC: Refer to "PC connection", page 140.
Printer: Refer to "Printer connection", page 146.
Sample changer: Refer to "Sample changer", page 172.
Refer to "Electrode connection", page 102.
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Contrast
The contrast of the display can be adjusted in the Main window.
•press0 to increase the contrast
•press7 to decrease the contrast
Proceed as follows to adjust the contrast of the ABU52 display from
the ION570:
• Display the Cell window.
• At the line Instrument, select the ABU52 (ABU1 or ABU2) you
want to adjust the display contrast.
Refer to "ABU1/ABU2", page 37.
Controlled by TTL IN
•press0 to increase the contrast
•press7 to decrease the contrast
Refer to "Auxiliary input", page 49.
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Copy electrode
This procedure is used to create an electrode by copying an existing
one.
1. Enter the Electrodes window.
2. Press 4 then 1.
3. In the Function field, select the function according to the
electrode type then press ✓, see "Electrode type", page 106.
4. Press ✓ .
5. Select From = Catalogue.
6. In the ID field, select an electrode name from the Catalogue list.
7. In the id field, you can identify the electrode by assigning a
second id name. This electrode will be called "ID id".
8. Press 1 to confirm then 2 Copy from electrode.
9. In the Library field, select the Preprogrammed or User list.
10. In the ID field, select the electrode to be copied from the list of
available electrodes.
11. Press 1 to confirm. The electrode is created and saved in the
User list.
If you selected the option Preprogrammed, the list is limited to
electrodes of the same type as the "copied" electrode.
If you selected User, the list is limited to electrodes having the
same function (pH, mV (i=0), mV (i>0), ISE, Conductivity, T°C,
Reference or Ground) as the "copied" electrode.
It is not necessary to select Catalogue to create an
electrode using the copy function. An electrode ID created from
Other can also use the copy function.
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Copy method
Copy reagent
This procedure is used to create a method by copying an existing one.
1. Switch to Main window.
2. Select Method.
3. Press 4 then 1.
4. Press 3 in New method menu.
5. Enter a method name.
6. Press 2 Copy from method.
7. In the Library field, select the Preprogrammed or User list.
8. In the ID field, select the method to be copied from the list of avail-
able methods.
9. Press 1 to confirm. The method is created and saved in the User
list.
This procedure is used to create a reagent by copying an existing one.
1. Enter the Reagents window.
2. Press 4 then 1.
3. Press ✓ in the New reagent menu.
4. Select From = Catalogue.
5. In the ID field, select a reagent name from the Catalogue list.
6. Enter the "approximate" titre for the reagent (between 0.001 and
99 999) in the Target titre field.
7. Enter the unit indicated on the reagent bottle (mM = mmol/l, M =
mol/l, mN = meq/l or N = eq/l).
8. Press 1 to confirm then 2 Copy from reagent.
9. In the Library field, select the Preprogrammed or User list.
10. In the ID field, select the reagent to be copied from the list of
available reagents.
11. Press 1 to confirm. The reagent is created and saved in the User
list.
It is not necessary to select Catalogue to create a
reagent using the copy function. A reagent ID created from
Other can also use the copy function.
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Coupled method
A Coupled method is a combination of methods performed in the
same beaker. When using a coupled method, the instrument runs all
these methods on the same sample.
If you wish to run a series of methods in different beakers, it is
necessary to program a Sequence instead of a Coupled method.
Example: Combination of method 1 and method 2.
The number of test portions (for example 3) is entered during
programming. The method is then repeated in the number of beakers
specified.
Sample 1
2
Method 2
Test portion 3
3
3
Method 2
Method 1
Test portion 1
1
1
Method 2
Method 1
= beaker number 1
1
Test portion 2
2
Method 1
Figure 10: Coupled method with three tests
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Create electrode
1. Enter the Electrode window.
2. Press 4 then 1.
3. Select the electrode function, see "Electrode type", page 106.
4. Press ✓ in the ID field.
5. Select From = Other.
The option From = Catalogue allows you to create an
electrode from a list of Radiometer Analytical electrodes.
6. Enter the electrode name (up to 16 alphanumeric characters).
7. In the Confirm creation screen:
• For pH, mV or ISE function electrodes only: select the electrode
type.
Refer to "Electrode type", page 106.
• For combined pH or single pH electrodes; enter the internal pH
(pH int) of the electrode.
Referto"pHint",page142.
• For combined pH, Metal/Redox or ISE electrodes or for a
Conductivity electrode; select if the electrode has a built-in
temperature sensor or not.
• For reference electrodes or combined pH, Metal/Redox, ISE
electrodes; enter the potential of the reference (in mV) versus the
Standard Hydrogen Electrode.
Refer to "Potential versus SHE", page 143.
• For ISE electrodes only; enter the ion valency and molar weight (in
g/mol).
Refer to "Valency", page 195.
• For conductivity cells only; enter the cable resistance (in Ω) and
capacitance (in pF).
Refer to "Cable resistance", page 54.
Refer to "Cable capacity", page 53.
8. Press 1 to create the electrode. The Edit electrode menu is
displayed. Enter the electrode definition parameters.
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Create method
Create reagent
1. Enter the Main window.
2. Select Method.
3. Press 4 then 1.
4. Press ✓ in the New method screen.
5. Enter a method name (up to 17 alphanumerical characters).
6. Press 1 to create the method. Go to Edit method screen and enter
the method parameters.
Refer to "Programming method", page 149.
1. Enter the Reagents window.
2. Press 4 then 1.
3. Press ✓ in the New reagent menu.
4. Select From = Other.
The option From = Catalogue allows you to create a reagent
from a list of commonly used reagents.
5. In the ID field, enter the reagent name (up to 14 alphanumeric
characters). It is advised to enter the chemical formula of the
reagent followed by its concentration (e.g. HCl 0.1).
Current value
6. Enter the "approximate" titre of the reagent (5 characters) in the
Target titre field.
7. Enter the unit indicated on the reagent bottle (mM = mmol/l, M =
mol/l, mN = meq/l or N = eq/l).
8. Press 1 twice to create the reagent. The Edit reagent menu is
displayed. Enter the reagent parameters.
This is the current sent to the Pt-Pt socket on the ION570. This
parameter is available if mV(i>0) has been selected for
Measurement in the Edit method menu.
Enter in:
Method parameters menu
Range available:
-1000 to +1000 µA in steps of 1 µA
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Curve
Curves data lost
- Cal. Data kept Methods kept
Customise
Select if you want to print:
• the E = f (pC = -log C) calibration curve at the end of each ISE
electrode calibration cycle,
• the GRAN = f(Volume) curve at the end of each test of a ISE
standard addition analysis.
Enter in:
Edit method > Printouts (ISE standard addition methods)
Edit electrode > Printouts (ISE electrodes)
The last curve data acquisition is lost. Generally, this error occurs
when the instrument is switched off while an analysis is in progress.
A name (max. 16 alphanumeric characters) can be assigned to the
ION570. This name will be displayed in the title bar of the Main window.
Date entry
Page 78
If required, a maximum of 4 lines (32 characters) is available to enter
personal information, or your company’s address. This information will
appear as a header at the start of the report printout.
Enter in:
Setup window > Customise
Enter current date in following format: dd:mm:yyyy.
Use the Up/Down arrow keys to jump to the month.
Enter in:
Setup menu > Configuration
MeterLab
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ION570 Reference Manual
Default parameters
Delay after addition
Reset the parameters programmed in the method, reagent, or
electrode. Use this command to reset the preprogrammed methods,
reagents or electrodes to the ION570’s default values.
Proceed as follows:
1. Display the Main, Reagent or Electrode window.
2. Press 4.
3. Select the method, reagent or electrode ID.
4. Press 3 Default parameters.
5. Press ✓ to confirm the reset.
This delay is counted down after a reagent addition. This time allows
the solution to stabilise after a reagent addition.
While an addition method is running, you can carry on
regardless this delay by pressing key Del.
Enter in:
Edit method > Parameters (ISE standard addition method
if Addition = Yes)
Delete electrode
Delete method
Range available:
00:00 to 99:59 min:s
1. Select the electrode to be deleted.
2. Press 4.
3. Press ✓ to confirm or ESC to leave the menu with deleting.
It is not possible to delete an electrode which is used in
another method or sequence. Modify the method or
sequence, e.g. change electrode iD or remove the electrode,
before deleting.
1. Select the method to be deleted.
2. Press 4.
3. Press ✓ to confirm or ESC to leave the menu with deleting.
It is not possible to delete a method which is part of a method
sequence or coupled method. Remove the method from the
sequence or from the coupled method before deleting.
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Delete reagent
Demand: Locked
1. Select the reagent to be deleted.
2. Press 4.
3. Press ✓ to confirm or ESC to leave the menu with deleting.
It is not possible to delete a reagent which is used in another
method or sequence. Modify the method or sequence, e.g.
change reagent iD or remove the reagent, before deleting.
Electrode calibration
The routine user is not allowed to bypass an electrode calibration
demand before continuing measurements. It means that the electrode
calibration periodicity has(have) been elapsed.
Reagent
The routine user is not allowed to bypass a reagent replacement
demand before continuing measurements. It means that the expiry
date of the reagent has elapsed.
QC sample analysis
If a QC sample periodicity has been reached, the next run of the
method must be performed on a QC sample.
Sequence edition
The routine user is not allowed to create, edit or delete sequence of
methods.
Enter in:
Setup menu > Access routine mode
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Demand: Unlocked
Electrode calibration
The routine user is allowed to bypass an electrode calibration demand
and continue measurements. This happens when the electrode
calibration periodicity has elapsed.
Reagent
The routine user is allowed to bypass a reagent replacement demand
and continue measurements. This happens when the expiry date of
the reagent has elapsed.
QC sample analysis
If a QC sample periodicity has been reached, the routine user is able
to run the method without having to use a QC sample.
Sequence edition
The routine user is allowed to create, edit or delete sequence of
methods.
Enter in:
Setup menu > Access routine mode
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Detailed
This parameter sets level of details of report printouts.
Detailed = Low
• The header only comprises the analysis name, time and date and
the instrument serial number. These data are printed on the same
line.
• Electrode calibration method: results are printed.
Detailed = Medium
This is the printout level selected by default.
• The header comprises the analysis name, time and date, the
instrument serial number and the laboratory coordinates.
• Electrode calibration method: results are printed.
Detailed = High
This is the printout level selected by default.
• The header comprises the analysis name, time and date, the
instrument serial number and the laboratory coordinates.
• Electrode data, electrode serial number, electrode calibration data
and results, burette serial number are printed.
Detection limit (C0)
Dilution (Yes/ No)
• The buffer or standard data (name and batch number, potential
value) are printed.
Enter in:
Edit method > Printouts
Edit electrode > Printouts
Refer to "Direct ISE measurement method - definition", page 83.
Select Dilution = Yes when you are analysing samples which
have been diluted. The Final dil. amount and Aliquot
are to be entered next.
Enter in:
Edit method > Sample menu
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Direct ISE
measurement
method definition
Measurement method using a selective electrode (ISE) of the ion you
want to determine the concentration.
In a Direct ISE measurement method, you must calibrate the ISE
electrode using 1 to 9 standard solutions of known concentration.
Refer to "Electrode calibration (ISE)", page 96.
0
If a calibration with 3 to 9 standards is carried out, E
, S and C0are
determined by non linear regression using the following equation:
0
E=E
+S25xT/T25(-log (C+C0)) where:
• E = potential measured in the sample,
0
= electrode standard potential,
•E
•S
= electrode response slope (sensitivity) at 25°C,
25
• T = temperature of the solution in K,
•T
= 298.16 K,
25
• C = concentration of sample,
= detection limit concentration. It is the “experimental detection
•C
0
limit of the electrode regarding the species under study
If a 1-point calibration is performed, only E
takes the S
sensitivity from the last calibration done or takes the
25
0
is calculated. The ION570
“.
theoretical value which depends on the ion valency (for example:
-59.16 mV for a positive monovalent ion). C
If a 2-point calibration is performed, E
0
the same equation as above but with C
is equal to 0.
0
and S25are calculated using
= 0. It is recommended to
0
perform a 2-point calibration in the linear response zone of the ISE
electrodre.
E (mV)
0
E
90
60
30
25
S
0
-30
-90
-120
-150
-180
-1-2
log (C)
C
0
-7
-5
-6
-4
-3
Refer to "Direct ISE measurement method - notes", page 84.
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Direct ISE measurement method - notes
The accuracy of the measurements using a Direct ISE method
depends on the following elements:
• The concentrations of the standards used for a 2-point calibration
must lie on either side of the samples to be measured.
• For calibration using more than 2 standards, one of the standard
concentration must lie in the non-linear response zone of the ISE
electrode.
• If you want to measure low concentrations (values situated in the
non-linear response zone), runa2or3-pointcalibrationinthenonlinear response zone of the electrode.
• It is recommended to measure sample concentrations above the
limit.
C
0
• A high value found for C
your standards and electrode).
• A similar ionic strength must be found in both standards and
samples (add a supporting electrolyte in the standards and
samples).
• The samples must not contain a significant amount of interfering
ions.
• Use the same temperature for your standards and samples
(thermostate the solutions).
may undergo false measurements (check
0
Direct ISE
measurement
method programmation
How to edit a Direct ISE measurement method?
see "Direct ISE measurement method - programmation", page 84.
How to run a Direct ISE measurement method?
Refer to "Running a method/sequence", page 170.
What is a Direct ISE measurement method?
see "Direct ISE measurement method - definition", page 83.
Proceed as follows to edit a Direct ISE measurement method:
1. From the Main window, press 4 then 2 Edit method.
2. For Mode, select Measurement.
3. For Measurement,selectISE Direct.
4. Define the other parameters of this measurement method.
What is a Direct ISE measurement method?
see "Direct ISE measurement method - definition", page 83.
How to run a Direct ISE measurement method?
Refer to "Running a method/sequence", page 170.
Direct measurements
Page 84
Refer to "Display measurement", page 86.
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Disconnect electrodes
Display contrast
Disconnect all connected electrodes.
Proceed as follows:
1. Press 2 in the Electrode window.
2. Press 2 Disconnect electrodes.
3. Disconnect electrode from rear panel.
4. Press ✓ to confirm.
5. Repeat steps 3 and 4 for all other electrodes to be disconnected.
Refer to "Contrast", page 72.
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Display measurement
Press 5 in the Electrode window.
The signal measured of a connected electrode in the current system
is displayed. If several electrodes are connected, select the electrode
at the ID line.
Depending on the type of electrode connected, the display shows:
• pH and corresponding potential difference in mV (pH electrodes)
• potential difference in mV (metal/redox or non-calibrated ISE
electrodes)
• concentration in the electrode calibration unit (calibrated ISE
electrodes)
E0 standard potential
• temperature in °C (temperature sensors)
• conductivity measured in mS/cm at sample temperature (conductivity cells). If the conductivity is not calibrated, the instrument
displays a conductivity with a cell constant value equal to 1 cm
The sample temperature is measured or is equal to 25 °C. There is
no temperature correction performed.
To get accurate measurements, it is therefore recommended to
calibrate the conductivity cell at a given temperature and
thermostat the sample to that temperature before running the
measurement.
Press 1 to apply or stop stirring.
Press Esc to stop measurements.
Refer to "Direct ISE measurement method - definition", page 83.
-1
.
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EC socket
6-pin DIN socket for connection of the conductivity cell with 2, 3 or 4
poles and a temperature sensor.
3
2
1
Pin layout:
4
Pin1:poleno.1
Pin2:poleno.2
6
Pin3:poleno.3
5
Pin 4 : pole n°4, also connected to pin no. 5
Pin 5 : 0 V (ground)
Pin 6 : temperature sensor
Potential imposed between poles 2 and 3: ±200 mV constant for all
conductance ranges.
The current passing through poles 1 and 4 is measured. The potential
between poles 1 and 4 cannot exceed 3 V in absolute value.
The following Radiometer Analytical conductivity cells can be
connected to the EC socket:
Conductivity
cell
Number
of poles
Built-in
temperature
Connection to
ION570
sensor
CDC566T (*) 4 Yes Direct connection
CDC866T (*) 4 Yes Direct connection
CDC641T (*) 2 Yes Direct connection
CDC511T 4 Yes Direct connection
CDC741T (*) 2 Yes Direct connection
CDC861T 4 Yes Direct connection
CDC565 4 No Direct connection
CDC749 2 No Direct connection
CDC745-9 (*) 2 No Via A94L136 cable
CDC267-9 2 No Via A94L136 cable
CDC241-9 2 No Via A94L136 cable
XE100 2 No Via A94L136 cable
Figure 11: Radiometer Analytical conductivity cells
(*) This conductivity cell is present in the ION570 electrode library (Catalogue list)
Conductivity cell with 2, 3 or 4 poles?
see the “Conductivity theory and practice“ guide, part no. D61M002.
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EC/pH
measurement
method definition
Using this method, conductivity and pH are measured simultaneously
in a same sample. This method uses a conductivity cell and a pH
combined electrode (or a separate pH and reference electrode).
Method parameters are those of a conductivity and a pH
measurement. Some parameters are common to the 2 types of
measurements such as the Acceptation time and the Maximum
stabilisation time. When both pH and the conductivity measurements
are stable, the ION570 displays the 2 results as R1 and R2.
Page 88
Refer to "EC/pH measurement method - programmation", page 89.
MeterLab
®
ION570 Reference Manual
EC/pH
measurement
method programmation
Proceed as follows to edit an EC/pH measurement method:
1. From the Main window, press 4 then 2 Edit method.
For Mode, select Measurement.
For Measurement, select EC/pH.
2. Press the Right arrow key, press 1.
Select a pH electrode and enter a pH measurement stability criterion.
Select a Conductivity cell and enter a conductivity measurement stability criterion.
Some parameters are common to both pH and Conductivity measurements
(Acceptation, Max. Stab time, Auxiliary output, Stirring).
Temp. correction , Temp. coef. and Reference Temp. parameters deal with
conductivity measurements.
3. Press the Esc key, press 3 and edit the Results parameters.
4. Press the Esc key, press 4 and edit the Printouts parameters.
What is an EC/pH measurement method?
Refer to "EC/pH measurement method - definition", page 88.
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Edit electrode menu
In this menu, you can rename the electrode (line ID), revise electrode
data, decide if you want to calibrate the electrode
(line Calibration request) and enter the electrode
calibration data if relevant.
To access:
1. Press 4 in the Electrode window.
2. Press 2 Edit electrode.
if an electrode calibration is requested, the following menus are
accessible using the arrow keys:
Calibration parameters.
Refer to "Electrode calibration parameters", page 100.
Calibration solutions.
Refer to "Solution menu", page 180.
Results.
Refer to "Results menu", page 164.
Printouts.
Refer to "Printouts menu", page 148.
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Edit method menu
In this menu, you can rename the method (line ID), revise and enter
method data.
To access:
1. Press 4 in the Main window.
2. Press 2 Edit electrode.
The following menus are accessible using the arrow keys:
Method parameters.
Refer to "Method parameters menu", page 132.
Sample.
Refer to "Sample menu", page 173.
Results.
Refer to "Results menu", page 164.
Printouts.
Refer to "Printouts menu", page 148.
QC Data
Refer to "QC data menu", page 151.
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Edit reagent menu
In this menu, you can rename the reagent (line ID) and revise
reagent data.
To access:
1. Press 4 in the Reagents window.
2. Press 2 Edit reagent.
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Edit sequence menu
Use this menu to program a sequence (add, insert, remove a method
from a sequence or delete the sequence). You can also specify the
number of times a method must be repeated within the sequence
(parameter Number of samples).
At the line Beakers: [F;L], the instrument displays the positions F and
L occupied by the first and last beakers In the sequence.
In the title bar, “x/y” (1/1) indicates the position "x" occupied by the
method in the sequence and "y" the total number of methods listed in
the sequence.
The ID and type of the selected method can not be modified at this
level. They are defined in the Add method or Insert method
menu.
To access:
1. Select Sequence or SAC Sequence for Working mode in the
Main window,
2. Press 2 Sequence/Sample stack,
3. Enter a name for the sequence,
4. Press 3 Edit sequence.
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Electrode calibration (Fixed mode, conductivity cell)
1. Select the method which uses the conductivity cell to be
calibrated.
2. Connect the electrode system,
see "Electrode connection", page 102.
3. Press 1 Calibrate electrodes in the Electrode window.
4. Select the conductivity cell from the list.
5. Press 1 to Run, and follow the messages on the display.
Measurements start in beaker no.1.
The ION570 displays the conductance
measured. The displayed temperature is
the temperature measured, entered or is
equal to 25°C according to the
calibration method programmed.
If you are not using a temperature probe and want to get
accurate measurements, it is recommended to thermostat
your standard beakers at the temperature you have entered
(or at 25°C).
Pay attention to the temperature range of the standard used:
see "Standard (conductivity standard)", page 182.
6. After stabilisation or at the end of the maximum stabilisation time,
the ION570 calculates the standard conductivity at the measured
or entered temperature. Then, the instrument calculates and
displays the cell constant.
7. Accept or reject the result then start a new calibration cycle (new
beaker of the same standard) or end the calibration.
Refer to "Result accepted (Yes/No)", page 161.
8. The cell constant (mean of all cycle results accepted) is saved
with the electrode.
To consult the calibration results, see "GLP-Archives menu",
page 115.
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Electrode calibration (Free mode, conductivity cell)
1. Select the method which uses the conductivity cell to be
calibrated.
2. Connect the electrode system,
see "Electrode connection", page 102.
3. Press 1 Calibrate electrodes in the Electrode window.
4. Select the conductivity cell from the list.
5. Press 1 to Run, and follow the messages on the display.
Measurements start in the user standard.
The ION570 displays the conductance
measured. The displayed temperature is
the temperature measured, entered or is
equal to 25°C according to the
calibration method programmed.
Note:
AnIDcanbeassignedtothestandard.
In this case, the standard ID entered
replaces the name
"User standard".
If you are not using a temperature probe and want to get
accurate measurements, it is recommended to thermostat
your standard beakers at the temperature you have entered
(or at 25°C).
6. After stabilisation in the user standard.
Press ✓ and enter the conductivity value
of your standard at the temperature
displayed.
Press 1 to confirm.
7. The ION570 calculates and displays the cell constant.
Accept or reject the result then start a new calibration cycle (new
beaker of the same standard) or end the calibration.
Refer to "Result accepted (Yes/No)", page 161.
8. The cell constant (mean of all cycle results accepted) is saved
with the electrode.
To consult the calibration results, see "GLP-Archives menu",
page 115.
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Electrode calibration (ISE)
Preparation of the calibration standards:
see "Calibration = Automatic", page 56,
see "Calibration = Manual", page 57.
1. Select the method which uses the electrode to be calibrated.
2. Connect the electrode system, see "Electrode connection", page
102.
3. Press 1 Calibrate electrodes in the Electrode window.
4. Select the electrode from the list.
5. Press 1 to Run, and follow the messages on the display.
The ION570 displays the potential
measured. The displayed temperature is
the temperature measured, entered or is
equal to 25°C according to the
calibration method programmed.
For a 3 to 9-point calibration, the E
sensitivity at 25°C and C0detection limit concentration are
S
25
0
standard potential,
calculated at the end of the calibration.
For a 2-point calibration, E
For a 1-point calibration, only E
0
and S25are calculated. C0=0.
0
is calculated, S25comes from
the last multi-point calibration performed or is equal to the default
value (59.16 mV/pC for a monovalent ion). C
0
=0.
Page 96
At the end of a calibration cycle, you can
display the E (mV) = f (pC = -log C)
calibration curve. The calibration points
are marked (here 3). To display the
curve of a calibration cycle, press 2 More
details then 4 Curve from the result
data display.
The calibration results are saved with the electrode.
To consult the calibration results, see "GLP-Archives menu",
page 115.
It is recommended to maintain all your standards at the same
temperature. Then the temperature entered at the start of a
calibration cycle is valid for all your standards.
MeterLab
®
ION570 Reference Manual
Electrode calibration (Fixed mode, pH electrode)
1. Select the method which uses the electrode to be calibrated.
2. Connect the electrode system, see "Electrode connection", page
102.
3. Press 1 Calibrate electrodes in the Electrode window.
4. Select the electrode from the list.
5. Press 1 to Run, and follow the messages on the display.
Measurements start in buffer no.1.
The ION570 displays the potential
measured. The displayed temperature is
the temperature measured, entered or is
equal to 25°C according to the
calibration method programmed.
6. After stabilisation of the measurement in buffer no.1:
The ION570 has recognised buffer no.1.
Enter the batch number for buffer no.2
and dip the electrodes in buffer no.2.
Measurements start in buffer no.2 and so
on.
A pH calibration can be performed over
1to5buffers.
The electrode zero pH and sensitivity are calculated at the end of
a multi-point calibration. For a 1-point calibration, only the zero pH
is calculated, the slope comes from the last calibration performed
or is equal to the default value (59.16 mV/pH unit). The calibration
results are saved with the electrode.
To consult the calibration results, see "GLP-Archives menu",
page 115.
It is recommended to maintain all your buffers at the same
temperature. Then the temperature entered at the start of a
calibration cycle is valid for all your buffers.
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Electrode calibration (Free mode, pH electrode)
1. Select the method which uses the electrode to be calibrated.
2. Connect the electrode system, see "Electrode connection", page
102.
3. Press 1 Calibrate electrodes in the Electrode window.
4. Select the electrode from the list.
5. Press 1 to Run, and follow the messages on the display.
Measurements start in buffer no.1.
The ION570 displays the potential
measured. The displayed temperature is
the temperature measured, entered or is
equal to 25°C according to the
calibration method programmed.
Note:
AnIDcanbeassignedtothepHbuffers.
In this case, the buffer ID entered
replaces the name
"Buffer n".
6. After stabilisation of the measurement in buffer no.1:
Press ✓ and enter the pH value of your
buffer at the temperature displayed.
Press 1 to confirm.
The calibration goes on with buffer no.2.
Enter the batch number for buffer no.2
and dip the electrodes in buffer no.2.
Measurements start in buffer no.2 and so
on.
A pH calibration can be performed over
1to5buffers.
The electrode zero pH and sensitivity are calculated at the end of
a multi-point calibration. For a 1-point calibration, only the zero pH
is calculated, the slope comes from the last calibration performed
or is equal to the default value (59.16 mV/pH unit). The calibration
results are saved with the electrode.
To consult the calibration results, see "GLP-Archives menu",
page 115.
It is recommended to maintain all your buffers at the same
temperature. Then the temperature entered at the start of a
calibration cycle is valid for all your buffers.
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Electrode calibration (sequence)
In a calibration sequence, the standard solution beakers are handled
automatically using a sample changer. A SAC80 or SAC90 Sample
Changer must be connected and declared in the Configuration menu.
1. Select the SAC Sequence or SAC Method option in the Main
window. This SAC Sequence or SAC Method must use the
electrode to be calibrated.
2. Connect the electrode system, see "Electrode connection", page
102.
3. Press 1 Calibrate electrodes in the Electrode window.
4. Select the electrode from the list of the electrode system.
5. Press 2 Calibration sequence.
6. Prepare the electrode calibration stack, see "Electrode
calibration stack", page 101.
7. Press Esc then 1 to run the calibration sequence. Follow the
messages on the display. The calibration results are calculated at
the end of the calibration. The calibration results are saved with
the electrode.
To consult the calibration results, see "GLP-Archives menu",
page 115.
Electrode calibration not required
Message appears at the start of a sequence, if a method sequence
has been programmed with an electrode calibration. The electrode
used has been programmed without calibration Calibration
request = No.
Go to Sequence/Sample stack, Edit sequence menu and
remove the electrode calibration method.
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Electrode calibration parameters
This menu contains parameters concerning the electrode calibration
method (measurement stabilisation criteria in particular).
To access:
1. From the Electrode window, press 4.
2. Select the electrode to be edited.
3. Press 2 Edit electrode and check that the Calibration
request = Fixed or Free option (pH electrode) or
Calibration = Manual or Automatic option (ISE
electrode) has been selected.
4. Edit the electrode calibration general parameters.
5. Use the LEFT/RIGHT arrow keys to move to the last Edit
electrode display.
6. Press 1 Calibration parameters.
Refer to "Calibration = Automatic", page 56.
Refer to "Calibration = Manual", page 57.
Refer to "Calibration request = Fixed", page 61.
Refer to "Calibration request = Free", page 62.
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