Philips OncoSignal User Manual

Pathway Activity Proling

OncoSignal

ER AR PI3K MAPK

The Philips Pathway Activity Proling OncoSignal Test for measuring ER, AR, PI3K and MAPK pathway activity is now available for research use in your molecular biology laboratory. The OncoSignal Test is performed using an RT-qPCR testing plate to measure mRNA levels of selected pathway target genes and includes access to a secure cloud-based environment for calculation and reporting of pathway activity scores.

OncoSignal is based on a unique knowledge-based approach and helps users gain insight into underlying tumor driving cell signaling pathways. The OncoSignal Test quantitatively measures the activity of the hormonal estrogen receptor (ER) and androgen receptor (AR) pathways, as well as the activity of PI3K and MAPK growth factor signal transduction pathways simultaneously in a single sample, using RNA from FFPE tissue of human origin.

OncoSignal Testing Plates

mRNA levels transcribed from target genes regulated by the pathway transcription factor are measured by means of the OncoSignal Testing Plates. The 96-well PCR testing plates are packaged in a box of six, for testing of six samples. Each testing plate contains carefully designed and optimized primers and probes for reliable detection of mRNA levels of the pre-selected signaling pathway target genes. Upon adding the sample RNA and one-step RT-qPCR mix to the testing plate, the test is run on a thermocycler under a specied PCR cycling program. Detailed protocols are provided in the Instructions for Use.

OncoSignal Report

PCR gene expression data are translated via a computational model into quantitative pathway activity scores. Each PCR data le uploaded in the cloud-based Philips ISPM OncoSignal portal undergoes a thorough quality check (QC) (e.g. controls for the quantity and quality of the RNA, and detection of correct plate lling), to ensure high-quality results. The OncoSignal Report presents an overview of the pathway activity scores per sample on a scale of 0-100 with respective 95% condence intervals. The report provides insights into the tumor driving pathways in the samples tested and enables a direct comparison of pathway activities between samples.

Workow OncoSignal Test

Select FFPE tumor

tissue block

1 2 3 4 5 6 7 8 9 10 11 12

Add PCR mix w ith extracted

RNA to test ing plate

Identify tumor area Macro dissect tumor area

Run the test on

a thermocycler

Features

• AR, ER, PI3K and MAPK pathway activity

• Highly reproducible quantitative pathway activity scores on a scale of 0-100 with condence intervals

• Translation of RT-qPCR data into pathway activity scores in a secure cloud-based environment

• Report providing pathway activity prole per sample

• Easy to implement in molecular biology laboratory

• Requires only 0.25 mm3 FFPE tissue per sample

• Fast turn-around-time (105 minutes)

Transfer tissue to

from unstained slide(s)

Process PCR data in

cloud-based env ironment

RNase-free tube

Generate report with

pathway activ ity scores

Additional reagents and equipment needed

• RNeasy FFPE Kit (Qiagen, cat. no. 73504)

• SuperScript III Platinum One-Step qRT-PCR Kit (Thermo Fisher Scientic, cat.no. 11732088)

• Bio-Rad CFX96 (Touch) Real-Time PCR Detection System

• Plate centrifuge

Applications

For information about example experimental designs, and interpretation of the OncoSignal Report, please request the application note.

Box with OncoSignal Testing Plates contains

• Six OncoSignal Pathway Activity Proling Testing Plates (96-wells plates with pre-designed and spotted qPCR assays)

• Six (+one spare) clear, adhesive PCR plate foils

• Quick Reference Guide

For Research Use Only - not for use in diagnostics procedures.

© 2020 Koninklijke Philips N.V. All rights reserved. Specications are subject to change without notice. Trademarks are the property of Koninklijke Philips N.V. (Royal Philips) or their respective owners.

DEC 2020

Order information

Please send your request to oncosignal@philips.com.

More information about the OncoSignal Pathway Activity Proling Test can be found at www.philips.com/oncosignal-test

Philips Electronics Nederland B.V. High Tech Campus 5 5656 AE Eindhoven The Netherlands

www.philips.com/oncosignal oncosignal@philips.com