Panasonic MCO-19AIC User Manual

Cell Culture CO2 and
CO2/O2 Incubators

MCO-19AIC/UV/UVH
MCO-19M/UV/UVH

Sterisonic GxP
Series Cell Culture
Incubators

Features:

• The industry’s most complete cell culture
solution for highly regulated applications or
conventional incubation.

• Safe, effective and documented 3 hour in situ H
decontamination for the fastest cycle turn-around.

Panasonic… the new name for SANYO

www.panasonic.com/biomedical

2O2

MCO-19AIC

MCO-19M
MCO-19AIC(UV)
MCO-19AIC(UVH)

MCO-19M(UV)

MCO-19M(UVH)

Sterisonic GxP Series Cell Culture CO2 and CO2/O2 Incubators

The industry’s most complete cell culture solution for highly regulated applications or conventional
incubation. Now with safe, effective and documented 3 hour in situ H
fastest turn-around and maximum availability.

Sterisonic GxP Selection

Feature

H2O2 Vapor Decontamination Standard Optional Optional Standard Optional Optional

SafeCell UV Decontamination Standard Standard Optional Standard Standard Optional

Single Beam, Dual Detector IR CO

Solid Zirconia O

Control N/A N/A N/A Standard Standard Standard

2

Sensor Standard Standard Standard Standard Standard Standard

2

MCO-

19AIC(UVH)

CO2 Incubators CO2/O2 Incubators

MCO-

19AIC(UV)

MCO­19AIC

decontamination for the

2O2

MCO-

19M(UVH)

MCO-

19M(UV)

MCO-

19M

Product Applications

The combination of Sterisonic GxP
incubator performance functions permit
use with condence in high-value cell
protocols among hard-to-grow cell lines,
cells highly sensitive to contamination
and vibration, ultra-sensitive media and
reagents, or protocols that require a
strict isolation and decontamination
between processes. These include but
are not limited to:

• Stem cell research

• Autologous tissue regeneration

and regenerative medicine

• In vitro fertilization

• Genomic and proteomic expression

• Esoteric plant and amphibian

cell culture

• Hypersensitive and transgenic

cell culture

• Low media volume microplate work

MC O -19AIC (UV H)

Designed for use with a variety
of standard cell culture vessels
and protocols.

• Interior components and adjustable
shelves are congured for easy access,
in situ decontamination and exible ar­rangement for a variety of applications.

• Four adjustable shelves are included
standard; maximum shelf capacity
is 15 shelves.

MC O -19 M

Multi-Gas Incubator, Oxygen
and CO2 Control

Ideal for in vitro fertilization (IVF), genetic
research, regenerative medicine and other
protocols that require CO
(hypoxic) or above-ambient oxygen control.

and sub-ambient

2

2 Sterisonic GxP Series Cell Culture Incubators

Sterisonic GxP Features and Benets

The Panasonic Sterisonic GxP is designed for a wide array of demanding and highly regulated applications in the biomedical, pharmaceutical,
medical research and clinical laboratory. Representing years of research, development and component testing, the Sterisonic GxP incorporates a
collective of mutually functional systems and design attributes to offer a holistic solution to cell culture protocols, from the most sophisticated to
more familiar and conventional processes.

Decontamination System

• The unique Sterisonic GxP H2O2 de­contamination system limits down­time to less than three hours when
total chamber decontamination with
verication is desired.

• All interior components and CO
sampling loop are decontaminated
in situ; no need for removal and
autoclaving.

• Active Background Contamination
Control ghts contamination while
cell culture protocols are in process.

The patented SafeCell

system scrubs interior airow
to destroy airborne and humidity pan
contaminants.

Exclusive inCu-saFe

®

enriched stainless steel interior
surfaces assure constant germicidal
protection.

Shelf brackets are formed
with an exaggerated angle
to minimize surface contact
with at shelves*.

UV

copper-

2

Control and Monitoring

• The Sterisonic GxP control and infor­mation center includes an intuitive pop­up menu, high resolution LCD
for inputs, outputs and performance
at-a-glance.

• Multi-point data logging offers
push-button graphical display. An
optional PC interface permits remote
transmission for GMP/GLP protocols
as required.

• Precise PID logic controls and
adjusts to all temperature, CO

set-

2

points and alarm parameters.

CO2 Control

Panasonic proprietary single­beam, dual detector infrared (IR2)

CO

sensor delivers precise CO2 control,

2

quick recovery following door openings,
and auto sampling with no moving parts.

• Continuous zero calibration is standard.

• An optional semi-automatic, one-point
calibration system is available. Catalog
No. MCO-SG; see accessories.

Zirconia O2 Control System,
MCO-19M Series

A zirconia oxygen sensor main-

tains sub-ambient O
1% to 18%. Additionally, enriched O
levels from 22% to 80% are enabled
with proper safety precautions.

• Concurrently the MCO-19M permits

a CO

range 0% to 20% via infrared

2

sensor.

• Nitrogen gas bubbler accelerates

recovery of chamber humidity levels
following door openings.

• An electronic P.I.D. control maintains

accurate temperature and gas set­points over the entire system range.

• The MCO-19M includes an automatic

gas switchover system that changes
from the primary to a secondary gas
cylinder for either oxygen or nitrogen;
an optional second gas switchover
system is available for CO

• Cabinet based on MCO-19 platform

with inCu-saFe

®

copper-enriched
stainless steel chamber, continuous
contamination control, patented D.H.A.
multi-point, air-jacketed temperature
control system, elevated relative hum­idity with integral water-level sensor.

levels from

2

.

2

2

MC O-19 M

MCO-19M shown with
four separate inner
doors with gaskets.

3

Sterisonc GxP Features and Benets

Temperature
and Humidity Control

• The patented Direct Heat and Air Jack­et heating system manages setpoint
temperature through multiple, variable
warming points under microprocessor
control.

• The humidity pan is easy to ll, easy
to clean; the automatic optical sensor
advises of low water level.

H2O2 Decontamination

The use of H2O2 decontamination
in biological safety cabinets and barrier
isolators is a popular alternative to
ethylene oxide (EtO) as a safer, more
efcient decontamination method.

H

has long been widely used in the

2O2

pharmaceutical industry. In aerospace
research, H
satellites and cleanrooms.

is used to decontaminate

2O2

Unlike conventional incubators, unique
features of the Panasonic Sterisonic
GxP incubator permit use of the H
process in situ with complete safety,
zero impact on adjacent equipment or
the environment, and speed to return
the incubator to service.

• The H

decontamination process

2O2

functions with the patented Pana­sonic SafeCell UV system. Following
a seven-minute H
circulation and dwell cycle, vaporization
is stopped and the SafeCell UV lamp
turned ON for up to ninety minutes.

• When exposed to UV light, the H
vapor breaks down into water and
oxygen, leaving only traces of water
droplets. These droplets automatically
condense onto a cooler section of the
interior oor for easy wipe-up.

• Throughout the entire cycle the
Sterisonic GxP airow system con­tinues to gently circulate interior air
assuring 100% vapor contact with all
interior surfaces, ultimately creating
a serial dilution of H
over the UV lamp.

vaporization,

2O2

as it passes

2O2

2O2

2O2

• Orientation of interior sample ports
of the single beam, dual detector IR
CO

sensor creates a slight Venturi

2

ow through the sample chamber, per­mitting total decontamination of the
CO

system at the same time.

2

• Shape and location of interior
components such as shelves, shelf
brackets, plenum covers and the
humidity pan permit the components
to remain in the chamber during the
decontamination process, conveniently
bypassing the need for a separate
autoclave cycle.

• Once the cycle is complete, the door
locking system is released; the inner
door can be opened, interior compo­nents repositioned and the incubator
is returned to service.

Ergonomic Cabinet Design

With reversible inner and outer doors,
a single Panasonic incubator offers the
industry's most exible installation
options available.

• Low prole cabinet with door-mounted
control panel permits easy access and
viewing.

• The outer door latch and door heater
cable is easily switched if a reverse
opening is required.

Sterisonic GxP Series H2O2 Decontamination Performance Value

3 Hours

Panasonic
Sterisonic

H2O

decontamination vs. high heat decontamination

2

= Uptime (Hours)

The documented a 3 hour in situ H2O2 sequence puts the fully decontaminated Sterisonic GxP available and
ready for use quicker than any other incubator worldwide.

4 Sterisonic GxP Series Cell Culture Incubators www.panasonic.com/biomedical

Brand X

=

Downtime (Hours)

14 Hours

Sterisonc GxP Features and Benets

• Cabinet knock-outs are pre-drilled
and tapped to eliminate drilling and to
simplify re-mounting of door hardware.

• The heated outer door closes against a
soft, easily cleaned magnetic gasket
designed to eliminate ambient air
shear across the glass inner door,
minimizing condensation.

• A door ajar alarm provides an audible
and visual warning if the outer door
is left open.

• A center-mounted key lock is located
beneath the door for added security.
(Model MCO-19AIC(UVH) only)

Shelves and Inventory
Management

Convenient space efcient inventory
management is simplied through a sys­tem of adjustable, extendable shelves.

• Inventory shelves and brackets
are formed from polished copper­enriched stainless steel, removable
without tools, and can remain
inside the incubator during the H
decontamination cycle or autoclaved
separately if desired.

2O2

• Shelves are perforated to permit natu­ral vertical air convection through and
around labware.

• Shelf brackets are formed with an
exaggerated angle to minimize surface
contact with at shelves. Brackets slip
easily into vertical supports that attach
to interior chamber walls with clear­ance sufcient to permit proper air
circulation.

Inner Door and Gasket

The inner design is critical to successful
contamination control technique.

• The inner gasket body forms an
effective thermal transition between
the ambient air and warm, humidied
incubator atmosphere, minimizing
condensation and eliminating moisture
traps which can harbor contaminants.

• The inner door gasket is a dual
durometer extrusion (two levels of
softness) from closed-cell silicone
to inhibit contamination.

• The gasket feather-edge allows
the inner glass door to close gently
against the chamber opening for
a tight peripheral seal.

MCO-19AIC(UVH) Double Stack Option

Stacking Doubles Capacity
in Same Footprint.

• The Sterisonic GxP cabinet is
designed for stacking, allowing one
unit to be positioned on top of
another, doubling interior volume
without additional oor space.

• The combination of stacking and
reversible doors offers the most
installation options possible.

• An optional roller base is recom­mended for stacked installations
to permit mobility if required;
see accessories.

• The entire inner door gasket is remov­able for cleaning and/or replacement
if required.

• Radiant heat from the outer door,
apportioned by the microprocessor
control as a function of the patented
Direct Heat and Air Jacket heating
cycle, automatically warms the inner
door glass in proportion to total heat
demand and condensation control.

Field Reversible Door

The eld-reversible door allows
universal installation using the left-hand
hinge (standard) or a right-hand hinge
modication

• The outer door includes a universal
nger grip at each side.

• Mounting holes for hinge hardware
are pre-drilled and capped with easily
removable trim plugs.

Elevated Humidity,
Low Water Level Warning

To avoid cell culture desiccation, the
Panasonic Sterisonic GxP CO
maintains ~95% RH at 37°C.

• Humidication is achieved by a com­bined forced-air and natural evapora­tion method enhanced by the Direct
Heat and Air Jacket heating system
base heater, and protected by an opti­cal water level indicator to warn of low
water in the removable humidity pan.

• The humidity pan removes easily for
regular cleaning and rell.

• When lled, the pan slides into place.
The optical sensor returns to position
and the SafeCell UV lamp destroys
contaminants introduced during the
process.

• For the H

in situ decontamination

2O2

cycle the humidity pan is repositioned
against the chamber wall to eliminate
the need for separate autoclaving.

incubator

2

5