Olympus Microscope User Manual

MICROSCOPE

COMPONENTS GUIDE

Choosing The Ideal UIS2 Optics Components For Your Equipment

2006-11

The wide range of Olympus components introduced here

allows users in such diverse fields as research, inspection and production

to take advantage of the quality, flexibility and

outstanding optical performance of the UIS2 Optical System.

That's why installing Olympus microscope components is,

quite simply, the right choice for your equipment.

1

CONTENTS

2

EYEPIECES/FILAR MICROMETER EYEPIECE --------------------------------- 33

Widefield eyepieces WHN10x, WHN10x-H,

CROSSWHN10x, WH15x --------------------- 33

Super widefield eyepieces SWH10x-H, MICROSWH10x,

CROSSSWH10x ------------------------------ 33

Filar micrometer eyepiece U-OSM --------------------------------------- 33

REVOLVING NOSEPIECES---------------------------------------------------- 34 — 35

Quintuple revolving nosepiece U-5RE-2-------------------------------------- 34
Sextuple revolving nosepiece with slider slot for DIC

U-D6RE--------------------------------------- 34

Sextuple revolving nosepiece with slider slot for DIC with ESD treatment

U-D6RE-ESD --------------------------------- 34

Septuple revolving nosepiece with slider slot for DIC

U-D7RE -------------------------------------- 34

Centerable quadruple revolving nosepiece with slider slot for DIC

U-P4RE -------------------------------------- 34

Centerable sextuple revolving nosepiece with slider slot for DIC

U-P6RE -------------------------------------- 34

Quintuple revolving nosepiece for BF/DF

U-5BDRE ------------------------------------ 35

Quintuple revolving nosepiece for BF/DF with slider slot for DIC

U-D5BDRE ----------------------------------- 35

Sextuple revolving nosepiece for BF/DF with slider slot for DIC/

U-D6BDRE ----------------------------------- 35

Centerable quintuple revolving nosepiece

U-P5BDRE ----------------------------------- 35

Adapter to mount BF objectives

BD-M-AD ------------------------------------ 35

VIDEO CAMERA ADAPTERS ------------------------------------------------- 36 — 37

C-mount video camera ports U-TV0.25xC, U-TV0.35xC-2,

U-TV0.5xC-3, U-TV0.63xC ------------------- 36

Video camera mount adapters U-CMAD3, U-BMAD, U-SMAD,

U-TMAD, U-FMT ----------------------------- 37

Video camera port U-TV1x-2 ------------------------------------ 37

MOTORIZED UNITS------------------------------------------------------------- 38 — 41

Motorized BF/DF reflected light illuminator+motorized Nomarski DIC sextuple
revolving nosepiece+100W halogen lamp housing

BX-RLAA+U-D6REMC+U-LH100-3 ---------- 38

Motorized universal reflected light illuminator

BX-RFAA ------------------------------------- 38

Motorized quintuple BD revolving nosepiece with slider slot for DIC

U-D5BDREMC ------------------------------- 39

Motorized sextuple revolving nosepiece with slider slot for DIC

U-D6REMC ---------------------------------- 39

Motorized centerable quintuple revolving nosepiece with slider slot for DIC

U-P5REMC ----------------------------------- 39
Control unit BX-UCB -------------------------------------- 39
Hand switch U-HSTR2 ------------------------------------ 39
Control box for motorized nosepiece and BF/DF illuminator

BX-REMCB ---------------------------------- 39
AC adapter for BX-REMCB U-ACAD4515 -------------------------------- 39
Active auto focus unit U-AFA1M ------------------------------------ 40
Motorized reflected filter wheel U-FWR --------------------------------------- 40
Motorized illumination with power focus

BXFMA-F ------------------------------------ 41

DEEP ULTRAVIOLET OBSERVATION SYSTEM -------------------------------- 42

UV248 compatible intermediate tube

U-UVF248IM --------------------------------- 42
UV quartz light guide U-UVF2FV/5FB ------------------------------- 42
UV248 compatible light source box + Mercury Xenon lamp housing

U-UVF248LB+U-LH80HBXE ----------------- 42

OPTICAL TERMINOLOGY ---------------------------------------------------- 43 — 46

WELCOME TO UIS2/UIS OPTICS ---------------------------------------------- 3 — 4

SYSTEM DIAGRAM --------------------------------------------------------------- 5 — 6

UIS2/UIS OBJECTIVE LENSES ----------------------------------------------- 7 — 18

M Plan SemiApochromat MPLFLN series -------------------------------- 8
Long WD M Plan SemiApochromat LMPLFLN series ------------------------------ 9
M Plan Achromat MPLN series --------------------------------- 10
LCD Long WD M Plan SemiApochromat

LCPLFLN-LCD series -------------------------11
M Plan Apochromat MPlanApo series -----------------------------12
Super Long WD M Plan Achromat SLMPlan series -------------------------------12
IR Long WD M Plan SemiApochromat

LMPlan-IR series -----------------------------13
IR M Plan SemiApochromat MPlan-IR -------------------------------------13
M Plan SemiApochromat BD MPLFLN-BD series ---------------------------14
M Plan SemiApochromat BDP MPLFLN-BDP series --------------------------15
Long WD M Plan SemiApochromat BD

LMPLFLN-BD series --------------------------16
M Plan Achromat BD MPLN-BD series ------------------------------17
M Plan Apochromat BD MPlanApo-BD --------------------------------18

MICROSCOPE SYSTEM BXFM ---------------------------------------------- 19 — 22

BXFM frame BXFM-F -------------------------------------- 19
BXFM BXFM-F+BXFM-ILH+BXFM-ILHSPU---------- 20
BXFM-S BXFM-F+BXFM-ILHS ------------------------ 21
Universal stand type 2 SZ2-STU2 ----------------------------------- 22
Compact stand U-ST ----------------------------------------- 22
Large stand SZ-STL -------------------------------------- 22

ILLUMINATION UNITS --------------------------------------------------------- 23 — 25

Reflected light illuminator for BF/DF

BX-RLA2 ------------------------------------- 23
Universal reflected light illuminatorBX-URA2 ------------------------------------ 23
Reflected light illuminators for BF BX-KMA/BX-KMA-ESD ----------------------- 24
Reflected light illuminator for BF U-KMAS ------------------------------------- 25

LAMP HOUSING & ACCESSORIES ----------------------------------------- 26 — 28

75W xenon apo lamp housing U-LH75XEAPO------------------------------- 26
100W mercury apo lamp housing U-LH100HGAPO ----------------------------- 26
100W mercury lamp housing U-LH100HG ---------------------------------- 26
100W halogen lamp housings U-LH100-3/U-LH100IR/U-LH100L-3 -------- 26
External power supply TH4-100/200 -------------------------------- 27
Hand switch TH4-HS -------------------------------------- 27
Extension cord U-RMT --------------------------------------- 27
DF converter for BX-URA2 U-RCV --------------------------------------- 27
Fiber adapter for reflected light observation

U-LGAD -------------------------------------- 27
Transmitted light guide adapter SZX-TLGAD ---------------------------------- 27
Light source LG-PS2 -------------------------------------- 28
Light guide LG-SF ---------------------------------------- 28
Double lamp house adapter U-DULHA ------------------------------------ 28

OBSERVATION TUBES -------------------------------------------------------- 29 — 30

Widefield binocular tube U-TR30-2 ------------------------------------ 29
Widefield binocular tube for IR U-TR30IR ------------------------------------ 29
Widefield erect image trinocular tube

U-ETR-4 ------------------------------------- 29
Single port tube with lens U-TLU ---------------------------------------- 29
Single port tube with lens for IR U-TLUIR ------------------------------------- 29
Super widefield trinocular tube U-SWTR-3 ----------------------------------- 30
Super widefield erect image trinocular tube

U-SWETR ------------------------------------ 30
Super widefield erect image tilting trinocular tube

MX-SWETTR --------------------------------- 30

INTERMEDIATE TUBES & ACCESSORIES ------------------------------- 31 — 32

Magnification changer U-CA ----------------------------------------- 31
Magnification changer 2x U-ECA --------------------------------------- 31
Trinocular intermediate attachment

U-TRU --------------------------------------- 31
Dual port U-DP ----------------------------------------- 32
Dual port 1x U-DP1xC ------------------------------------- 32
Eyepoint adjuster U-EPA2 -------------------------------------- 32
Arrow pointer U-APT --------------------------------------- 32

light between the objective lens and tube lens,
allowing the creation of user-specific or task­specific optical systems. To establish real flexibility
with such a system, it is necessary to eliminate the
occurrence of coma aberration.

*In UIS2/UIS objective lenses, the parfocal distance is designed

at 45mm and the focal length of the tube lens is 180mm.

Basic dimensions of UIS2/UIS optical system

The UIS2/UIS optical system optimally corrects
aberration with a dedicated telan lens and an
eyepiece so that the coma aberration and flatness
are not degraded even when the telan lens exit
pupil position is changed by changing the
objective lens and telan distance. This makes it
possible to use a distance of 50mm to 170mm
from objective lens mounting position to the single
port tube with lens.

*Coma aberration: refer to the optical terminology at the end of

this document.

What's infinity-corrected optics?

UIS2/UIS optics is an infinity-corrected optical
system — in other words, a system in which light
passes from the specimen through the objective
lens without forming an image along the way.
Instead, it travels in the form of infinity parallel rays
to the tube lens. The tube lens is where the
intermediate image is formed, whereas in finite­corrected optics, this is done by the objective lens.

Advantages of infinity-corrected optics

This system, known as "infinity-corrected optics",
offers a number of advantages:

• There is no change in magnification even when
the distance between the objective lens and tube
lens is altered.

• With the total magnification remaining constant,
there is no image aberration — even when
prisms or sliders are interposed between the
objective lens and the tube lens.

As thousands of users have found by experience,
these advantages are crucial to composing the
ideal microscope optical system. What's more, it
is even possible to freely insert or remove
intermediate attachments in the parallel rays of

Figure 1 Infinity-corrected and
finite-corrected optical system principles

Infinity-corrected optical system

Finite-corrected optical system

Parallel light beam

Eyepiece

Eyepiece

UIS/UIS2
objective lens

Objective
lens

Tube lens Intermediate

image

Intermediate

image

3

WELCOME TO UIS2/UIS OPTICS

UIS2/UIS:
The System That Maximizes The Advantage Of Infinity-Corrected Optics

Figure 3 Basic dimensions of UIS2/UIS2
optical system

Objective lens

U-TLU
(Single port tube with lens)

Image

Recommended distance

50-170mm

* 40mm

45mm

* 84mm

57.6mm 102mm

*Basic dimensions when our revolving nosepiece and illuminator are
combined. When the position of the illuminator above is changed,
illumination performance cannot be maintained.

Figure 2 Advantages of Infinity-corrected
optical system

Infinity-corrected optical system Finite-corrected optical system

Objective
lens

Tube lens

Objective
lens

4. Lightening

Weight has been reduced to approximately 2/3
that of conventional products by using an
aluminum objective lens barrel cover. This has
the effect of lightening the load on the devices at
objective lens up/down, suppressing vibrations
by lowering the inertial force at objective lens
switching, etc. (MPLFLN series, LMPLFLN
series)

5. Adoption of eco-lens

The glass materials of UIS2 objective lenses are
all lead- and cadmium-free eco-glass.

Features of UIS2 objective lenses

UIS2 objective lenses ensure compatibility (screw
diameter, optical performance) with the UIS optical
system and have the following features compared
to conventional objective lenses.

1. Wavefront aberration control

The Olympus UIS2 objective lenses set a new
standard, with wavefront aberration control in
addition to common performance standards of
N.A. and W.D. Olympus challenges farther
highest order optics which has not been fulfilled
by the conventional standards. We offer
excellent performance objective lenses by
minimizing the aberrations that lower resolution.

*Wave front aberration: refer to the optical terminology at the
end of this document.

2. Objective lenses with excellent image

parcentricity

High power SemiApochromatic UIS2 objective
lenses make the centration tolerance between
objective lenses on the microscope nosepiece
keep the image within the enter of the field of
view even with digital cameras. (50x or higher
power in both MPLFLN and LMPLFLN series)

3. Improvement of color reproducibility

UIS2 objective lenses realize natural color
reproduction without any chromatic shifts using
stringently selected high transmittance glass
and advanced coating technology that provides
high transmittance which is flat over an ultra­wide band wavelength. In addition, since the
total optical system, including the tube lens is
designed to reproduce a natural color, clear
images faithful to the specimen are obtained
even with digital imaging.

WELCOME TO UIS2/UIS OPTICS

4

Based on our conviction that the UIS2/UIS
system is the best way to maximize the
advantages of infinity-corrected optical
systems, we confidently recommend the
UIS2/UIS-featured Olympus microscope units
for all your high-precision needs in research,
inspection and production equipment.

* Refer to the Olympus home page for detailed

objective lenses specifications.

5

SYSTEM DIAGRAM

5

6

SHUTTERSHUTTER

5

6

SHUTTER

U-AN

U-AN360-3
U-AN360IR

LG-SF

LG-PS2

U-LH100HGAPO
U-LH100HG

U-LH75XEAPO

U-LH100-3
U-LH100L-3
U-LH100IR

Super widefield trinocular
observation tubes

Widefield trinocular
observation tubes

U-TLU
U-TLUIR

U-25ND6, U-25ND25,
U-25LBD, U-25IF550,
U-25L42, U-25FR,
U-BP1100IR, U-BP1200IR

U-PO3
U-POIR

SZ2-STU2

U-LGAD

TH4-100 TH4-HS

U-STSZ-STL

BXFM-ILH

BXFM-ILHS

BXFM-F

BXFM-ILHSPU

WHN
Eyepieces

SWH
Eyepieces

BX-RLA2

ND

FS

AS

BX-URA2

U-RCV

U-KMAS

SZX-TLGAD

U-DULHA

BX-KMA/
BX-KMA-ESD

U-POTP3

FS

AS

U-TV0.25xC U-TV0.35xC-2 U-TV0.5xC-3 U-TV0.63xC

U-TMAD

U-BMAD U-SMAD

U-FMT

U-CMAD3

Video camera

C-mount

Video camera
S mount 2/3"

Video camera

F mount

Video camera
B mount 2/3"

U-TV1x-2

Revolving nosepiece

(Refer to pages 34-35)

Refer to pages 19-21

Refer to page 22

Refer to pages 23-28

Refer to pages 29-30

Illumination systems and power supply

Focusing units

Stands

Refer to pages 36-37

Video system

Observation/single tubes
and eyepieces

BXFM SYSTEM DIAGRAM

*Different types may be offered in each area.

6

SYSTEM DIAGRAM

MOTORIZED UNIT SYSTEM DIAGRAM

BXFM-A SYSTEM DIAGRAM

See manual

U-D5BDREMC

U-D6REMC

U-FWO

BX-RFAA

U-AN360RAF

U-AFA1M

BX-RLAA

U-LH100-3
U-LH100L-3

U-FWR

Intermediate tubes

(Refer to pages 31-32)

Observation tubes

(Refer to pages 29-30)

U-DICRHC

U-DICR

U-DICRH

U-AN360-3

U-25ND6,
U-25ND25
U-25LBD
U-25IF550
U-25L42
U-25FR

U-PO3

U-AN

U-HSTR2

BX-UCB*

U-ZPCB

Z board

U-POTP3

PC

BX2BSW

Control software

Mirror units

U-LH75XEAPO
U-LH100HGAPO
U-LH100HG

BF/DF objective lenses

BF objective lenses

BD-M-AD

Refer to pages 38-40

Refer to page 26

Refer to pages 7-18Refer to

page 35

* BX-REMCB is also available for BX-RLAA + motorized revolving nosepiece control (refer to page 39)

Video system

(Refer to pages 37-38)

Observation tubes

(Refer to pages 29-30)

U-AFA1M

BXFMA-F

U-D6REMC
U-P5REMC
U-D5BDREMC

Objective lenses

U-FWR

ø32filter

Auxiliary lens
(provided with the BXFMA-F)

U-LGAD

Refer to pages 26-27

U-LH100-3
U-LH100L-3

U-IFFH

U-FH

U-RMT

LG-SF

Refer to page 26

U-LH100HG
U-LH75XEAPO

See manual

BX-UCB

U-ZPCB

Z board

Refer to pages 27-28

PC

Refer to page 41

Power source

LG-PS2

U-HSTR2

7

UIS2/UIS OBJECTIVE LENSES

MPL(Plan)

FLN-100 BD

■ Objective lens series list

■ Meaning of abbreviations shown on objective lens

■ Objective lens notation

■ Features of objective lens series

None: Brightfield
BD: Brightfield/darkfield
BDP: Brightfield/darkfield/

polarizing

IR: IR
LCD: LCD

None:

UIS

N: UIS2

Number:

Objective lens
magnification

Series Magnification BF DF DIC*1POL FL F.N. (Field Number) Remarks

UIS2 MPLFLN 1.25/2.5 \ 1.25x: 22 / 2.5x: 26.5 Use together with polarizer and analyzer

recommended

5/10/20/50/100 \\U \\

*2

26.5
LMPLFLN 5/10/20/50/100 \\L \\ 26.5
MPLN 5/10/20/50/100 \ 22
LCPLFLN-LCD 20/50/100 \\L 26.5 For LCD

UIS MPlanApo 20/50/100 \\

*3

U \ 26.5
SLMPlan 20/50 \ 26.5
LMPlanIR/MPlanIR 5/10/20/50/100 *

4

\ 22 For near-IR observation

UIS2 MPLFLN-BD 5/10/20/50/100/150 \\\U \\

*2

26.5

MPLFLN-BDP 5/10/20/50/100 \\aU a\

*2

26.5
LMPLFLN-BD 5/10/20/50/100 \\\L \\ 26.5
MPLN-BD 5/10/20/50/100 \\ 22

UIS MPlanApo-BD 100 \\\U \ 26.5

*

1

DIC prism U-DICR: UM/LM position, U-DICRHC: LM position fixed, U-DICRH: UM position fixed. *25~20x: U excitation also possible

*

3

50x: DIC observation not applicable *4MPlanIR: available 100x only \: Responds a: Optimally responds BF: Brightfield DF: Darkfield

DIC: Differential Interference Contrast POL: Polarized light FL: Fluorescence

M: Metallurgical (no cover)
LM: Long working distance

metallurgical use

SLM: Super long working

distance metallurgical use

LC: Observation through

substrate

None: Achromat/

Corrects aberration at 2 wavelengths of
blue and red

FL: SemiApochromat/

Corrects chromatic aberration in the
visible range (violet~red)

APO: Apochromat/

Optimally corrects chromatic aberration
in the entire visible band (violet~red)

PL: Plan/

Corrects field
curvature of
the periphery of
the image plane

Magnification

Objective lenses series abbreviation (PL: Plan)
N.A. (Numerical Aperture)

Field Number

For brightfield observation

Infinity-corrected optical system

Cover glass thickness (no cover)

a MPLFLN series: M Plan SemiApochromat — P 8

Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration.
The lineup consists of 7 objective lenses ranging from 1.25x to 100x, and secures a W.D. of
1mm or longer. Since the exit pupil position of the 5x-100x objective lenses is standardized,
the position of the DIC prism does not have to be switched when changing the magnification.
For ultra low magnifications (1.25x, 2.5x), use together with analyzer and polarizer of the
reflected light illuminator.

a LMPLFLN series: Long WD M Plan SemiApochromat — P 9

Long working distance Plan SemiApochromat objective lenses, giving high-level correction
for chromatic aberration. Suitable with samples having a height difference and in preventing
collision, as the working distance is long. Also, since the exit pupil position of the 5x-100x
objective lenses is standardized, the position of the DIC prism does not have to be switched
when changing the magnification.

a MPLN series: M Plan Achromat — P 10

Plan Achromat objective lenses providing excellent image flatness up to F.N. 22.

a LCPLFLN-LCD series: LCD Long WD M Plan SemiApochromat — P 11

Perfect objective lens series for observation of LCD panels and other samples through a
glass substrate. Aberration correction matched to the glass thickness is accomplished using
a correction ring.

a MPlanApo series: M Plan Apochromat — P 12

Highest class Plan Apochromat objective lenses that maximize performance in brightfield
observation. All aberrations are corrected at the highest level, while providing high N.A.

a SLMPlan series: Super Long WD M Plan Achromat — P 12

Plan Achromat objective lenses with high magnification and super long working distance.
Two magnifications, 20x and 50x are available. For 5x or 10x objective lenses, select from
the LMPLFLN Series.

a LMPlan-IR series: IR Long WD M Plan SemiApochromat — P 13

MPlan-IR: IR M Plan SemiApochromat — P 13

IR objective lenses which compensate for aberrations from visible to near infrared light.
Ideal for the observations of semiconductor interiors and the back surface of a chip package
as well as CSP bump inspection.

a MPLFLN-BD series: M Plan SemiApochromat BD — P 14

Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration.
The series secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-150x
objective lenses is standardized, the position of the DIC prism does not have to be switched
when changing the magnification.

a MPLFLN-BDP series: M Plan SemiApochromat BDP — P 15

Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration.
The series secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-100x
objective lenses is standardized, the position of the DIC prism does not have to be switched
when changing the magnification. The BDP series optimizing brightfield/darkfield and
polarized light characteristics is perfect for Nomarski DIC and polarized light observations.

a LMPLFLN-BD series: Long WD M Plan SemiApochromat BD — P 16

Long working distance Plan SemiApochromat objective lenses, giving high-level correction
for chromatic aberration. Suitable with samples having a height difference and in preventing
collision, as the working distance is long. Also, since the exit pupil position of the 5x-100x
objective lenses is standardized, the position of the DIC prism does not have to be switched
when changing the magnification.

a MPLN-BD series: M Plan Achromat BD — P 17

Plan Achromat objective lenses providing excellent image flatness up to F.N. 22.

a MPlanApo BD: Plan Apochromat BD — P 18

Highest class Plan Apochromat objective lens that maximize performance in brightfield and
darkfield observations. All aberrations are corrected at the highest level, while providing
high N.A.

UIS2 OBJECTIVE LENSES

8

M Plan SemiApochromat

MPLFLN series

Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The lineup consists of 7 objective
lenses ranging from 1.25x to 100x, and secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-100x objective
lenses is standardized, the position of the DIC prism does not have to be switched when changing the magnification.
For ultra low magnifications (1.25x, 2.5x), use together with analyzer and polarizer of the reflected light illuminator.

ø28

ø20.32

ø28

ø20.32

ø20.32

ø20.32

ø30

ø29

ø28.6

ø30

ø29

ø24.5

ø26

ø20.9

ø26

ø14.5

4.9

WD=3.5

45

(41.5)

41.2

45

4.9

(34.3)

WD=10.7

31.6
45

4.5

WD=20

WD=11

(25)

22.55
(34)

4.5

45

28.41

ø20.32

ø20.32 ø20.32

ø10.7

ø15.2
ø17.8

ø26

ø7.8
ø15.2
ø17.8

ø26

ø26

ø12.1

WD=3.1

4.5

(41.9)

45

35.1

WD=1

45

4.8

(44)

38.4

42.4

42.61

4.8

45

WD=1

(44)

41.78

40.8

36.8

MPLFLN1.25x* MPLFLN2.5x* MPLFLN5x MPLFLN10x

MPLFLN20x MPLFLN50x MPLFLN100x

MPLFLN 1.25x* 0.04 3.5 145 122 12.5 17.6 870 —— —
MPLFLN 2.5x* 0.08 10.7 72 106 25 8.8 220 25 10.6 220
MPLFLN 5x 0.15 20.0 36 51.5 50 4.4 59 50 5.3 59
MPLFLN 10x 0.30 11.0 18 68.1 100 2.2 15 100 2.7 15
MPLFLN 20x 0.45 3.1 9 70.4 200 1.1 5.2 200 1.3 5.1
MPLFLN 50x 0.80 1.0 3.6 89.9 500 0.44 1.3 500 0.53 1.3
MPLFLN 100x 0.90 1.0 1.8 90.9 1000 0.22 0.73 1000 0.27 0.73

Numerical

Aperture

Working distance

(mm)

Focal distance

f (mm)

Weight

(g)

Total

magnifications

Practical field

of view (mm)

Depth of

focus (µm)

Total

magnifications

Practical field
of view (mm)

Depth of

focus (µm)

UIS2 objective lenses

Widefield eyepiece WHN10x

Field Number 22

Super widefield eyepiece SWH10x

Field Number 26.5

Objective lens

(magnification)

Screw: W20.32x0.706 (0.8"x1/36") * To be available in the beginning of 2007

Unit: mm

UIS2 OBJECTIVE LENSES

9

Long WD M Plan SemiApochromat (WD: Working Distance)

LMPLFLN series

Long working distance Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration.
Suitable with samples having a height difference and in preventing collision, as the working distance is long.
Also, since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have
to be switched when changing the magnification.

LMPLFLN 5x 0.13 22.5 36 50 50 4.4 70 50 5.3 70
LMPLFLN 10x 0.25 21.0 18 54 100 2.2 18 100 2.7 18
LMPLFLN 20x 0.40 12.0 9 73 200 1.1 6.1 200 1.3 6.1
LMPLFLN 50x 0.50 10.6 3.6 77 500 0.44 2.5 500 0.53 2.5
LMPLFLN 100x 0.80 3.4 1.8 94 1000 0.22 0.87 1000 0.27 0.87

Numerical

Aperture

Working distance

(mm)

Weight

(g)

Focal distance

f (mm)

Total

magnifications

Practical field

of view (mm)

Depth of

focus (µm)

Total

magnifications

Practical field

of view (mm)

Depth of

focus (µm)

UIS2 objective lenses

Widefield eyepiece WHN10x

Field Number 22

Super widefield eyepiece SWH10x

Field Number 26.5

Objective lens

(magnification)

Screw: W20.32x0.706 (0.8"x1/36")

Unit: mm

45

4.5

WD=21

(24)

ø22.4

ø26

ø26

ø17

ø26

22.1

ø15.2

45

WD=22.5

4.9

(22.5)

ø25.4

22.2

4.8

(33)WD=12

45

29.31

ø20.32ø20.32ø20.32

ø26

ø18.2

WD=3.4 (41.6)

45

4.7

ø20.32ø20.32

ø12.5

ø15

ø18.1

41.1

37.26

ø26

45

WD=10.6

(34.4)

4.9

30.4

LMPLFLN5x LMPLFLN10x LMPLFLN20x

LMPLFLN50x LMPLFLN100x

10

UIS2 OBJECTIVE LENSES

Unit: mm

M Plan Achromat

MPLN series

Plan Achromat objective lenses providing excellent image flatness up to F.N. 22.

MPLN 5x 0.10 20.0 36 64 50 4.4 98
MPLN 10x 0.25 10.6 18 80 100 2.2 18
MPLN 20x 0.40 1.3 9 111 200 1.1 6.1
MPLN 50x 0.75 0.38 3.6 113 500 0.44 1.4
MPLN 100x 0.90 0.21 1.8 116 1000 0.22 0.73

Numerical

Aperture

Working distance

(mm)

Focal distance

f (mm)

Weight

(g)

Total

magnifications

Practical field of

view (mm)

Depth of focus

(µm)

UIS2 objective lenses

Widefield eyepiece WHN10x

Field Number 22

Objective lens

(magnification)

Screw: W20.32x0.706 (0.8"x1/36")

ø20.32

ø20.32

ø20.32

ø6
ø11.9
ø15.8

ø24

ø21
ø24

ø24

ø16

ø12

ø10.5

45 4.5

23.4

(25)

WD=20

WD=10.6 (34.4)

4.545

32.71

28.8

33.6

4.5

(43.7)

45

WD=1.3

37.2

41.3

42.4

ø20.32

ø20.32

ø6

ø11.9
ø15.8

ø24

ø4.4

ø11.6
ø15.6

ø24

(44.62)WD=0.38

4.5

45

37.2

41.3

42.62

WD=0.21 (44.79)

45

4.5

38.7

42.8

43.16

MPLN5x MPLN10x MPLN20x

MPLN50x MPLN100x

UIS2 OBJECTIVE LENSES

11

LCD Long WD M Plan SemiApochromat

LCPLFLN-LCD series

Perfect objective lens series for observation of LCD panels and other samples through a glass substrate.
Aberration correction matched to the glass thickness is accomplished using a correction ring.

Objective lens LCPLFLN20xLCD LCPLFLN50xLCD LCPLFLN100xLCD
Corresponding glass thickness (mm) 0-1.2 0-1.2 0-0.7
Correction ring indication 0 0.7 1.2 0 0.7 1.2 0 0.5 0.7
Working distance (mm) 8.3 7.8 7.4 3.0 2.5 2.2 1.2 0.98 0.9
Correction system Correction ring Correction ring Correction ring

LCPLFLN20xLCD*** 0.45 7.8 9 146 200 1.1 5.2 200 1.3 5.2
LCPLFLN50xLCD*** 0.70 2.5 3.6 170 500 0.44 1.6 500 0.53 1.6
LCPLFLN100xLCD*** 0.85 0.9 1.8 185 1000 0.22 0.79 1000 0.27 0.79

Numerical

Aperture

Working distance**

(mm)

Focal distance

f (mm)

Weight

(g)

Total

magnifications

Practical field

of view (mm)

Depth of

focus (µm)

Total

magnifications

Practical field

of view (mm)

Depth of

focus (µm)

UIS2 objective lenses

Widefield eyepiece WHN10x

Field Number 22

Super widefield eyepiece SWH10x

Field Number 26.5

Objective lens

(magnification)

Screw: W20.32x0.706 (0.8"x1/36") **The figure shown here is the value when the correction ring indication is 0.7. *** To be available in the beginning of 2007

Unit: mm

WD=0.9 (43.638)

45.238 *

43.4

41.6

27.5

20

ø25

ø20.32

ø15.2

ø29.5

4.5

(36.738)

45.238 *

WD=7.8

t=0.7

t=0.7

t=0.7

WD=2.5

4.5

ø20.32

ø15
ø25

ø29.5

ø31

36.55

34.75

24.5

20

45.238 *

4.8

(42.038)

ø20.32

ø12.77

ø17.84

ø25

ø29.5

ø31

ø31

40.45

38.65

25

20

LCPLFLN20xLCD*** LCPLFLN50xLCD*** LCPLFLN100xLCD***

* Value at glass thickness 0.7mm observation

UIS OBJECTIVE LENSES

12

Unit: mm

ø5.6

ø16.5

ø20
ø28

WD=0.9

34.7

38.7

40.1

(44.1)

45 4.5

ø20.32

36.8

40.7

41.7

(44.7)WD=0.3

45

4.5

ø8
ø16.6
ø20.2

ø28

ø20.32

ø5.3

ø16.5
ø20.2

40.7

41.5

(44.65)WD =0.35

45 4.5

ø20.32

ø28

36.8

MPlanApo20x MPlanApo100xMPlanApo50x

M Plan Apochromat

MPlanApo series

Highest class Plan Apochromat objective lens that maximize performance in brightfield observation.
All aberrations are corrected at the highest level, while providing high N.A.

MPlanApo 20x 0.60 0.9 9 150 200 1.1 3.7 200 1.3 3.7
MPlanApo 50x 0.95 0.3 3.6 150 500 0.44 1.0 500 0.53 1.0
MPlanApo 100x 0.95 0.35 1.8 150 1000 0.22 0.67 1000 0.27 0.67

Numerical

Aperture

Working distance

(mm)

Focal distance

f (mm)

Weight

(g)

Total

magnifications

Practical field
of view (mm)

Depth of

focus (µm)

Total

magnifications

Practical field
of view (mm)

Depth of

focus (µm)

UIS objective lenses

Widefield eyepiece WHN10x

Field Number 22

Super widefield eyepiece SWH10x

Field Number 26.5

Objective lens

(magnification)

Screw: W20.32x0.706 (0.8"x1/36")

Unit: mm

Super Long WD M Plan Achromat

SLMPlan series

Plan Achromat objective lenses with high magnification and super long working distance.
Two magnifications, 20x and 50x are available. For 5x or 10x objective lenses, select from the LMPLFLN series.

22.7

23.7

(23.95)

45

ø20.32

ø17.8
ø24.2

ø26

WD=21.05

4.9

26.4

28.1

29

(29.96)

45

ø20.32

ø17
ø19.3
ø22.7

ø26

4.9

WD=15.04

SLMPlan20x SLMPlan50x

SLMPlan 20x 0.35 21.0 9 73 200 1.1 7.2 200 1.3 7.2
SLMPlan 50x 0.45 15.0 3.6 91 500 0.44 2.9 500 0.53 2.9

Numerical

Aperture

Working distance

(mm)

Focal distance

f (mm)

Weight

(g)

Total

magnifications

Practical field
of view (mm)

Depth of

focus (µm)

Total

magnifications

Practical field
of view (mm)

Depth of

focus (µm)

UIS objective lenses

Widefield eyepiece WHN10x

Field Number 22

Super widefield eyepiece SWH10x

Field Number 26.5

Objective lens

(magnification)

Screw: W20.32x0.706 (0.8"x1/36")

13

UIS OBJECTIVE LENSES

IR Long WD M Plan SemiApochromat/IR M Plan SemiApochromat

LMPlan-IR series/MPlan-IR

IR objective lenses which compensate for aberrations from visible to near infrared light.
Ideal for the observations of semiconductor interiors and the back surface of a chip package as well as CSP bump inspection.

34.3

30.3

22.5

ø

21

ø

26

(26.5)

WD=18.5

25

23

ø12.4

ø

15

ø

22

45

4.5

(36.9)

ø

20.32

ø

20.32

ø10.4

ø

18

ø

26

45

4.5

45

WD=20

(25)

ø

20.32

WD=8.1

ø

26

4.5

ø14.8

ø

18.12

(44.7)

ø12.5

4.5

45

WD=0.3

43.4

42.6

36.8

ø

20.32

ø

15

37

45

4.5

ø

20.32

ø4.95
ø12.2

45

ø

26

ø

26

(39)

WD=6

37.5

36.9

32.9

ø12.5
ø15.6

ø

18

4.7

ø

20.32

(41.6)

WD=3.4

40.8

ø

26

LMPlan5xIR LMPlan10xIR LMPlan20xIR

LMPlan50xIR LMPlan100xIR

Unit: mm

MPlan100xIR

LMPlan 5xIR 0.10 20.0 36 73 50 4.4 98
LMPlan 10xIR 0.25 18.5 18 73 100 2.2 18
LMPlan 20xIR 0.40 8.1 9 110 200 1.1 6.1
LMPlan 50xIR 0.55 6.0 3.6 115 500 0.44 2.2
LMPlan 100xIR 0.80 3.4 1.8 122 1000 0.22 0.87
MPlan 100xIR 0.95 0.3 1.8 130 1000 0.22 0.67

Numerical

Aperture

Working distance

(mm)

Focal distance

f (mm)

Weight

(g)

Total

magnifications

Practical field of

view (mm)

Depth of focus

(µm)

UIS objective lenses

Widefield eyepiece WHN10x

Field Number 22

Objective lens

(magnification)

Screw: W20.32x0.706 (0.8"x1/36")

UIS2 OBJECTIVE LENSES

14

M Plan SemiApochromat BD (BD:Brightfield/Darkfield)

MPLFLN-BD series

Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration.
The series secures a W.D. of 1mm or longer. Since the exit pupil position of the 5x-150x objective lenses is standardized, the
position of the DIC prism does not have to be switched when changing the magnification.

MPLFLN 5xBD 0.15 12.0 36 95.5 50 4.4 59 50 5.3 59
MPLFLN 10xBD 0.30 6.5 18 82.8 100 2.2 15 100 2.7 15
MPLFLN 20xBD 0.45 3.0 9 87.7 200 1.1 5.2 200 1.3 5.2
MPLFLN 50xBD 0.80 1.0 3.6 99.8 500 0.44 1.3 500 0.53 1.3
MPLFLN 100xBD 0.90 1.0 1.8 98.9 1000 0.22 0.73 1000 0.27 0.73
MPLFLN 150xBD 0.90 1.0 1.2 104.8 1500 0.15 0.6 1500 0.18 0.6

Numerical

Aperture

Working distance

(mm)

Focal distance

f (mm)

Weight

(g)

Total

magnifications

Practical field
of view (mm)

Depth of

focus (µm)

Total

magnifications

Practical field
of view (mm)

Depth of

focus (µm)

UIS2 objective lenses

Widefield eyepiece WHN10x

Field Number 22

Super widefield eyepiece SWH10x

Field Number 26.5

Objective lens

(magnification)

Screw: W26x0.706

Unit: mm

ø26 ø26 ø26

ø16
ø22.4
ø29.5

ø32

ø22

ø16.8

ø26.2

ø27
ø32

ø22

ø17

ø27.5
ø28.5

ø32

4.5

WD=12

WD=6.5

45

(33)

31.5

31

30.25
(38.5)

4.545

37

36.5

34.1

4.5

(42)

45

39.5

39

35.97

WD=3

ø26

ø26

ø26

ø20

ø27.2

ø32

ø20

ø27.2

ø32

ø27.2

ø20

ø32

(44)

4.5

WD=1

45

41

4.5

WD=1

WD=1

45

(44)

41

4.5

45

(44)

41

MPLFLN5xBD MPLFLN10xBD MPLFLN20xBD

MPLFLN50xBD MPLFLN100xBD MPLFLN150xBD

15

UIS2 OBJECTIVE LENSES

Screw: W26x0.706

Unit: mm

MPLFLN 5xBDP 0.15 12.0 36 95.5 50 4.4 59 50 5.3 59
MPLFLN 10xBDP 0.25 6.5 18 83.3 100 2.2 18 100 2.7 18
MPLFLN 20xBDP 0.40 3.0 9 88.5 200 1.1 6.1 200 1.3 6.1
MPLFLN 50xBDP 0.75 1.0 3.6 100.5 500 0.44 1.4 500 0.53 1.4
MPLFLN 100xBDP 0.90 1.0 1.8 101.5 1000 0.22 0.73 1000 0.27 0.73

Numerical

Aperture

Working distance

(mm)

Focal distance

f (mm)

Weight

(g)

Total

magnifications

Practical field

of view (mm)

Depth of

focus (µm)

Total

magnifications

Practical field

of view (mm)

Depth of

focus (µm)

UIS2 objective lenses

Widefield eyepiece WHN10x

Field Number 22

Super widefield eyepiece SWH10x

Field Number 26.5

Objective lens

(magnification)

MPLFLN5xBDP MPLFLN10xBDP MPLFLN20xBDP

MPLFLN50xBDP MPLFLN100xBDP

ø26 ø26 ø26

ø22.4

ø16

ø29.3

ø32

ø26

ø22

ø16.8

ø27
ø32

ø27.5

ø22

ø17

ø28.5

ø32

45

WD=12

WD=6.5

WD=3

4.5

(33)

31.5

31

30.25
(38.5)

4.545

37

36.5

34.1

4.5

(42)

39.5

39

35.97

45

ø26

ø26

ø32

ø27.5

ø20

ø32

ø27.5

ø20

WD=1

WD=1

(44)

4.545

41

4.545

(44)

41

M Plan SemiApochromat BDP (BDP:Brightfield/Darkfield/Polarizing)

MPLFLN-BDP series

Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration. The series secures a W.D. of
1mm or longer. Since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism
does not have to be switched when changing the magnification. The BDP series optimizing brightfield/darkfield and polarized
light characteristics is perfect for Nomarski DIC and polarized light observations

UIS2 OBJECTIVE LENSES

16

Long WD M Plan SemiApochromat BD

LMPLFLN-BD series

Long working distance Plan SemiApochromat objective lenses, giving high-level correction for chromatic aberration.
Suitable with samples having a height difference and in preventing collision, as the working distance is long.
Also, since the exit pupil position of the 5x-100x objective lenses is standardized, the position of the DIC prism does not have to be
switched when changing the magnification.

LMPLFLN 5xBD 0.13 15.0 36 81 50 4.4 70 50 5.3 70
LMPLFLN 10xBD 0.25 10.0 18 84 100 2.2 18 100 2.7 18
LMPLFLN 20xBD 0.40 12.0 9 86 200 1.1 6.1 200 1.3 6.1
LMPLFLN 50xBD 0.50 10.6 3.6 85 500 0.44 2.5 500 0.53 2.5
LMPLFLN 100xBD 0.80 3.3 1.8 102 1000 0.22 0.87 1000 0.27 0.87

Numerical

Aperture

Working distance

(mm)

Focal distance

f (mm)

Weight

(g)

Total

magnifications

Practical field
of view (mm)

Depth of

focus (µm)

Total

magnifications

Practical field
of view (mm)

Depth of

focus (µm)

UIS2 objective lenses

Widefield eyepiece WHN10x

Field Number 22

Super widefield eyepiece SWH10x

Field Number 26.5

Objective lens

(magnification)

Screw: W26x0.706

Unit: mm

LMPLFLN5xBD LMPLFLN10xBD LMPLFLN20xBD

LMPLFLN50xBD LMPLFLN100xBD

45

WD=15

(30)

ø26

ø16.2
ø22.4

ø28

ø29.5

ø32

26.6

26.2

24

WD=10 (35)

45

ø26

ø15.5
ø21.9
ø28.2

ø30
ø32

32.3

32

30.2

4.5

5

WD=12

(33)

45 4.5

ø26

ø22
ø23
ø28
ø30

ø32

32.5

32.2

31.3

WD=3.3 (41.7)

45 4.7

ø26

ø21
ø29

ø32

37.7

37.3

36.5

ø30.3

ø26

ø23

ø20.7

ø28
ø32

WD=10.6

45

4.8

(34.4)

33

32.7

31.8

UIS2 OBJECTIVE LENSES

17

M Plan Achromat BD

MPLN-BD series

Plan Achromat objective lenses providing excellent image flatness up to F.N. 22.

MPLN 5xBD 0.10 12.0 36 137 50 4.4 98
MPLN 10xBD 0.25 6.5 18 155 100 2.2 18
MPLN 20xBD 0.40 1.3 9 162 200 1.1 6.1
MPLN 50xBD 0.75 0.38 3.6 157 500 0.44 1.4
MPLN 100xBD 0.90 0.21 1.8 160 1000 0.22 0.73

Numerical

Aperture

Working distance

(mm)

Focal distance

f (mm)

Weight

(g)

Total

magnifications

Practical field of

view (mm)

Depth of focus

(µm)

UIS2 objective lenses

Widefield eyepiece WHN10x

Field Number 22

Objective lens

(magnification)

Screw: W26x0.706

Unit: mm

MPLN10xBD MPLN20xBD

MPLN100xBD

ø26 ø26

ø26

ø32

ø30.5

ø29.3

ø16

ø32

ø32

ø29

ø23.6

ø17

ø27

ø26

ø22

ø16.8

4.5

WD=12

45

(33)

31.5

31

30.25

45

(38.5)WD=6.5

4.5

37

36.5

34.01

(4.5)

WD=1.3 (43.7)

45

42.5

41

ø26

ø26

ø29
ø32

ø23.1

ø20.7

ø10

ø29
ø32

ø27

ø23

ø10

WD=0.38

(44.62)

45

4.5

43.68

42.5

41

WD=0.21 (44.79)

45

4.5

43.71

42.5

41

43.33

MPLN5xBD

MPLN50xBD

18

UIS OBJECTIVE LENSES

ø26

41

42.5

43.2

(44.69)

4.5

WD=0.31

ø10

ø23
ø27
ø29
ø32

45

MPlanApo100xBD

M Plan Apochromat BD

MPlanApo-BD

Highest class Plan Apochromat objective lens that maximize performance in brightfield and darkfield observations.
All aberrations are corrected at the highest level, while providing high N.A.

MPlanApo100xBD 0.9 0.31 1.8 180 1000 0.22 0.59 1000 0.27 0.59

Numerical

Aperture

Working distance

(mm)

Focal distance

f (mm)

Weight

(g)

Total

magnifications

Practical field
of view (mm)

Depth of

focus (µm)

Total

magnifications

Practical field
of view (mm)

Depth of

focus (µm)

UIS objective lenses

Widefield eyepiece WHN10x

Field Number 22

Super widefield eyepiece SWH10x

Field Number 26.5

Objective lens

(magnification)

Screw: W26x0.706

Unit: mm

BXFM frame

BXFM-F

Widely used system that allows use in combination with fiber illumination, motorized revolving nosepiece and telan lens unit.
Can easily be integrated into other equipment. Attach to the equipment by rear bolt mounting screw or pillar mounting hole.

19

MICROSCOPE SYSTEM BXFM

Weight: 1.9kg Unit: mm

98 110

80

58.5

0.5

(13)

(13)

55

23 7

4–M4 depth9

4–M4 depth7

Pillar mount hole center

66.2

16 17 17 34

36

35

82

4-M8 depth8

(Bolt mount screw)

0.5 17 100

124

36

ø32H8

(Pillar mount hole)

84

Stroke

2A00002

MICROSCOPE SYSTEM BXFM

20

BXFM

BXFM-F+BXFM-ILH+BXFM-ILHSPU

Accommodates the reflected light brightfield/darkfield and fluorescence illuminators.

Weight: 3.2kg Unit: mm

11

Light axis

Light axis

165

130

83

45 40

72

23 7

3.5

Holder mounting position

Pillar axis

Revolving nosepiece mounting position

Objective lens

mounting position

Stroke

Specimen position

180

587

220 180

249

130

87.5
83

ø 32

40

3.5

45

165

11

17-47(stroke)

124

Specimen
surface

BXFM combination sample
BXFM-F+BXFM-ILH+BXFM-ILHSPU+TR30-2+BX-RLA2+U-LH100L-3

Weight: 8.2kg (exclude objective lens) Unit: mm* For installation dimensions, refer to those for the BXFM-F (page 19).

MICROSCOPE SYSTEM BXFM

21

ø32

169

(Stroke)

19-49

124

84

208

290

204045

106 187

92.5

Specimen surface

BXFM-S combination sample
BXFM-F+BXFM-ILHS+TR30-2+U-KMAS+U-LH100L-3

Weight: 5.5kg (exclude objective lens) Unit: mm

BXFM-S

BXFM-F+BXFM-ILHS

Compact focusing unit suitable for building into existing equipment.

Weight: 2.4kg Unit: mm

20

Stroke

723

Light axis

45 40 84

141

59

106

Holder mounting position

Pillar axis

Revolving nosepiece
mounting position

Objective lens
mounting position

Specimen position

* For installation dimensions, refer to those for the BXFM-F (page 19).

MICROSCOPE SYSTEM BXFM

22

U-ST

Compact stand

Weight: 5kg Unit: mmWeight: 1.8kg

106

73 143

88

ø110

133

ø32

49 133

20

35

ø40

130

503

ø25

Ø32

214~435

622

350

300

180º

90

50.5

30º

250

300

Stands

A wide variety of stands are available to suit different applications and purposes.

Item Specifications

1 Diameter of focusing arm or

ø32mm

fixing section of tube

2 Vertical pole diameter ø40mm
3 Horizontal poles diameters ø25mm

(both upper and lower poles)

4 Stroke Horizontal: 234mm,

Vertical: 205mm

5 Movement range Horizontal: 541 (435+106) mm max.

(Vertical pole —
BXFM-S optical axis)

6 Maximum specimen weight Forward: 10kg

(within 90-degree area)
Transverse direction: 6kg
Backward direction: 7kg
(at maximum stroke)

7 Weight 30kg

Major specifications

SZ2-STU2

Universal stand type 2

SZ-STL

Large stand

160

320

400

ø32

17.5

45

46

267

* The rotation angle of the horizontal arm can restrict to 90 degrees with stopper.

ILLUMINATION UNITS

23

Universal reflected light illuminator

BX-URA2

Suitable for observations ranging from brightfield to fluorescence.
Six mirror units can be attached to this reflected light illuminator simultaneously.

Reflected light illuminator for BF/DF

BX-RLA2

ND filters are linked when exchanging between brightfield and darkfield.

Unit name Description Weight (g)
U-25LBD LBD filter slider 20
U-25IF550 IF550 filter slider 20
U-25ND6 ND filter 20
U-25ND25 ND filter 20
U-25FR Frost filter slider 20
U-25L42 UV-cut filter 20
U-PO3 Polarizer slider for reflected light 71
U-POTP3 Polarizer slider for reflected light 71

with tint plate
U-AN360-3 360° rotatable analyzer slider 79
U-AN Analyzer slider for reflected light 50
U-DICR DIC slider for reflected light 130
U-DICRH DIC slider for reflected light 130

(high resolution type)
U-DICRHC DIC slider for reflected light 130

(high contrast type)

Accessories

Unit name Description Weight (g)
U-25LBD LBD filter slider 20
U-25IF550 IF550 filter slider 20
U-25ND6 ND filter 20
U-25ND25 ND filter 20
U-25FR Frost filter slider 20
U-25L42 UV-cut filter 20
U-PO3 Polarizer slider for reflected light 71
U-POTP3 Polarizer slider for reflected light 71

with tint plate
U-AN360-3 360° rotatable analyzer slider 79
U-AN Analyzer slider for reflected light 50
U-DICR DIC slider for reflected light 130
U-DICRH DIC slider for reflected light 130

(high resolution type)
U-DICRHC DIC slider for reflected light 130

(high contrast type)
U-MBF3 Mirror unit for reflected brightfield 80
U-MDF3* Mirror unit for reflected darkfield 80
U-MDIC3 Mirror unit for reflected DIC 80
U-MBFL3 Mirror unit for reflected brightfield, 80

for high intensity light source
U-MWUS3 Fluorescence mirror unit for 80

reflected (U excitation)
U-MWBS3 Fluorescence mirror unit for 80

reflected (B excitation)
U-MWGS3 Fluorescence mirror unit for 80

reflected (G excitation)

Accessories

Unit: mm

Weight: 3.8kg

ø84

88

84

3.5

41

367

261

41

12

(17.9°)

76

11.6

17

11.6

86.8

27.2

(152)

135°

Illuminator
mounting
position

Revolving
nosepiece
mounting
position

31.5144

3046

ø75

88

108

38.7

56.829.4

16.2

12

13

11.8

1726

84

3.5

41

335

265

45

30107.5

7.5

17.5
35

Illuminator
mounting
position

Revolving
nosepiece
mounting
position

* U-RCV (DF converter for BX-URA2) is needed with darkfield observation.

Weight: 3.4kg

ILLUMINATION UNITS

24

170 (Distance to the light axis)

Convex section(2-4) for positioning

3.5

Less than R6

21

82 (Revolving nosepiece relief dimension)

5

5.5

(6)

(11)

2.5

52 (Revolving nosepiece relief dimension)

100 (±0.1)

62 (±0.1)

37 ±0.1 (45° location face)

Location face

Location face

2

45° (±10')

4-M5 depth12 or more

36 (±0.1)

MOUNTING DIMENSIONS OF ILLUMINATORS (BX-RLA2, BX-URA2 and BX-KMA/BX-KMA-ESD)

Unit: mmFix illuminator using four M5 screws and projection for fastening.

Reflected light illuminators for BF

BX-KMA/BX-KMA-ESD

Enables brightfield, Nomarski DIC and simple polarizing observations. ESD model is also available.

Weight: 3.1kg

Unit: mm

* Combine SZX-TLGAD when using fiber illumination.

88

ø70

108

Cable length 260.5mm

312.5

250

30

84

3.5

41

Illuminator
mounting
position

Revolving
nosepiece
mounting
position

Unit name Description Weight (g)
U-25LBD LBD filter slider 20
U-25IF550 IF550 filter slider 20
U-25ND6 ND filter 20
U-25ND25 ND filter 20
U-25FR Frost filter slider 20
U-25L42 UV-cut filter 20
U-PO3 Polarizer slider for reflected light 71
U-POTP3 Polarizer slider for reflected light 71

with tint plate
U-AN360-3 360° rotatable analyzer slider 79
U-AN Analyzer slider for reflected light 50
U-DICR DIC slider for reflected light 130
U-DICRH DIC slider for reflected light 130

(high resolution type)
U-DICRHC DIC slider for reflected light 130

(high contrast type)

Accessories

LAMP HOUSING & ACCESSORIES

25

Reflected light illuminator for BF

U-KMAS

Very compact reflected light illuminator with reduced depth.

Weight: 1.2kg

Unit: mm

ø75

88

198

155

21

64

6

21

Unit name Description Weight (g)
U-25LBD LBD filter slider 20
U-25IF550 IF550 filter slider 20
U-25ND6 ND filter 20
U-25ND25 ND filter 20
U-25FR Frost filter slider 20
U-25L42 UV-cut filter 20
U-PO3 Polarizer slider for reflected light 71
U-POTP3 Polarizer slider for reflected light 71

with tint plate
U-AN360-3 360° rotatable analyzer slider 79
U-AN Analyzer slider for reflected light 50
U-DICR DIC slider for reflected light 130
U-DICRH DIC slider for reflected light 130

(high resolution type)
U-DICRHC DIC slider for reflected light 130

(high contrast type)

Accessories

LAMP HOUSING & ACCESSORIES

26

Lamp housings

Various different lamp housings are available, for use with different light sources: choose to suit the intended purpose.

Unit: mm

U-LH100HGAPO

100W mercury apo lamp housing

U-LH100HG

100W mercury lamp housing

U-LH75XEAPO

75W xenon apo lamp housing

U-LH100-3/U-LH100IR/U-LH100L-3

100W halogen lamp housings

Cable length 2,000mm Accepted lamp: UXL-75XB Weight: 3.1kg Cable length 2,000mm Accepted lamp: USH-103OL Weight: 2.7kg

Cable length U-LH100-3: 290mm

U-LH100IR: 290mm
U-LH100L-3: 800mm

Accepted lamp: 12V100WHAL (high intensity lamp)

12V100WHAL-L (long life lamp)

8

11575

180.5

169 (depth dimension for installation)

93

25°

55 6

30.2

30°

40.820

(148.5)

83.5
6565

60130

(30.2)(148.5)

93

169

(depth dimension for installation)

180.5

130

18.5

8

83.5

65

25°

65

40.8

30°

11575

20

135

(depth dimension for installation)

146.5

85.5 10737

Weight: 880g

* Power supply unit (BH2-RFL-T3 or U-RFL-T200) and power cable (UYCP) are necessary

for 100W mercury lamp housings. These items are sold separately.
BH2-RFL-T3: dimensions 120(W)x290(D)x225(H), weight approx 5kg/
U-RFL-T200 (for EU countries): dimensions 150(W)x295(D)x200(H), weight approx. 4.8kg

*Power supply unit (AH2-RX-T or U-RX-T200) and power cable (UYCP) are necessary for

75W xenon lamp housing. These items are sold separately.
AH2-RX-T: dimensions 120(W)x290(D)x186(H), weight approx. 4kg/
U-RX-T200 (for EU countries): dimensions 115(W)x195(D)x260(H), weight approx. 3kg
Note: Supplied by Olympus Life and Material Science Europa GmbH and its business
partners.

* External power supply (TH4-100 or TH4-200) and power cable (UYCP) are necessary for 100W halogen

lamp housings. These items are sold separately. For TH4-100/200 installation dimensions, refer to the next page.

LAMP HOUSING & ACCESSORIES

27

31.5 (dimensions
for installation)

Darkfield light excluding tube
which is built into the BX-URA2.

(42.5)

2137.5

ø59

ø75

43

Lamp housing accessories

For the 100W halogen lamp, the external power supply TH4-100/200 with an intensity adjustment switch and an ON/OFF switch,
both are located close to the operator's hand, are provided. All Olympus reflected light illuminators can be used with fiber illumination.

Unit: mm

1,700

Unit: mm

Weight: 315g

U-RCV

DF converter for BX-URA2

U-RMT

Extension cord

Weight:200 g

Light guide mount hole ø12

Weight: 135g

SZX-TLGAD

Transmitted light guide adapter

U-LGAD

Fiber adapter for reflected light

observation

Light guide mount hole ø12

Weight: 390g

Weight: 2.2kg

TH4-100/200

External power supply

TH4-HS

Hand switch

Weight: 140g

14.575 200

120

125

42 38

18.5

77

Cable length:
2,000mm

26

24 (dimension
for installation)

44

ø30

ø12

ø49

* Mountable with BX-KMA/BX-KMA-ESD only.

51 (dimension
for installation)

62.5

ø67

ø59

ø32

LAMP HOUSING & ACCESSORIES

28

Unit: mmUnit: mm

Weight: 1.6kg

Weight: 210g

LG-PS2*

Light source

LG-SF

Light guide

U-DULHA

Double lamp house adapter

8

76

235

251

10

10

126

86

130

ø15 (Light guide mounting position)

Groove: Width3, Depth1

10

ø10.1

ø13

ø12

ø25

ø15

30

31

61

25

20

1,000

*The types of model varies by country in use.

202

171

82

88

ø140

Weight: 1.2kg

OBSERVATION TUBES

29

Weight: 350g

ø60

57.6

• For attachable video camera adapters, refer to video camera adapters system diagram page (pages 5-6).

U-TLU

Single port tube with lens

U-TLUIR

Single port tube with lens for IR

Widefield trinocular observation tubes

Trinocular observation tubes with widefield of view. Compatible with F.N. 22.

33.5 16 120

199.9

163.1

103.9

92

104.9

51.6

62.5

U-ETR-4

Widefield erect image trinocular tube

U-TR30-2/

Widefield binocular tube

U-TR30IR

Widefield binocular tube for IR

Single port tube with lens

When the visual observation is not needed and only video observation is required, a single port tube with a built-in telan lens
can be attached directly to the video port.

Unit: mm

Unit: mm

U-TR30-2 22 30 50-76 100/0, 20/80, 0/100 Inverted 1600
U-TR30IR 22 30 50-76 100/0, 0/100 Inverted 1600
U-ETR-4 22 30 50-76 100/0, 0/100 Erect 1900

Field Number

(F.N.)

Inclination angle

(degree)

Interpupillary distance

(mm)

Name

Light path selector

(eyepiece/video port)

Observation image

Weight

(g)

*Length marked with an asterisk (*) may vary according to interpupillary distance. The distance for figure shown is 62mm.

175

47.9

62.5 (IR: 64.5)

43.5

186.6 (IR: 188.9)

92.5* (IR: 93.9)

95.8

60.6

150.5

18

59.65

30

OBSERVATION TUBES

117.223

61.981

139.400

88.000

2.837

70.330

149.081

20° 0' 0"

42° 0' 0"

95.929

101.929

318.527

328.338

337.718

72.200

Super widefield trinocular observation tubes

Trinocular observation tubes with super widefield of view. Compatible with F.N. 26.5.

55.6

79.6

180.8

49.968.6

98.8

173.8

220.8

82.3*

63.1

14

U-SWTR-3

Super widefield trinocular tube

MX-SWETTR

Super widefield erect image tilting trinocular tube

U-SWETR

Super widefield erect image trinocular tube

U-SWTR-3 26.5 24 50-76 100/0, 20/80, 0/100 Inverted 2300
U-SWETR 26.5 24 50-76 100/0, 0/100 Erect 4200
MX-SWETTR 26.5 0-42 50-76 100/0, 0/100 Erect 4200

Unit: mm

Field Number

(F.N.)

Inclination angle

(degree)

Interpupillary distance

(mm)

Name

Light path selector

(eyepiece/video port)

Observation image

Weight

(g)

*Length marked with an asterisk (*) may vary according to interpupillary distance. The distance for figure shown is 62mm.

73

83.4

92.9

6298.6

201.9

248.9

76.4*

2241936.5

Intermediate tubes

Various accessories for various observation need.

31

INTERMEDIATE TUBES & ACCESSORIES

Unit: mm

Weight: 1.3kg

U-ECA

Magnification changer 2x

Provides 1x and 2x intermediate magnifications.

Weight: 1.3kg

U-CA

Magnification changer

Provides 1x, 1.2x, 1.6x and 2x intermediate magnifications.

Weight: 1.3kgBI:PT=100:0/20:80

U-TRU

Trinocular intermediate attachment

Intermediate attachment which divides the light path,

allowing attachment of both digital and video cameras.

ø140

150

88

52

37

183.9

106.9

52

37

150

ø140

58.2

ø138

96 42

ø70

ø75

45

42

INTERMEDIATE TUBES & ACCESSORIES

32

Weight: 1kg

U-DP

Dual port

Use this intermediate tube to divide the light path.

Weight: 500g

U-DP1xC

Dual port 1x

Combine with U-DP to obtain a 1x image.

Weight: approximately 500g

U-EPA2

Eyepoint adjuster

Raises eyepoint by 30mm.

Weight: 1.2kg

U-APT

Arrow pointer

Projects an arrow into the field of view.

Unit: mm

88

38

51(mount face)

151

57

ø140

1-32UN

17.53

4.5

170.5(mount face)
182

ø44

ø25

ø44

ø30

0.92

(tolerence from light axis)

120

(45°)

45.3

15V0.2A

21.2 115

(8°)

45

89

30

88

Transmitted side port: side port = 100:0
Transmitted side port: side port = 70:30 (with use of U-MBF3)

Light path selector by mirror unit

EYEPIECES/FILAR MICROMETER EYEPIECE

33

Eyepieces

Eyepieces for UIS2 optical system.

Filar micrometer eyepiece

U-OSM

Used for precise measurement in the field of view.

WHN10x

Widefield eyepiece

WHN10x-H

CROSSWHN10x

Widefield eyepieces

WH15x

Widefield eyepiece

SWH10x-H

MICROSWH10x

CROSSSWH10x

Super widefield eyepieces

(25) 18.7

43.7

29.6

ø36.5

ø41

36.8

EP

ø39

EP

ø43.2

ø46.2

ø30

(28.5)

53.2

41.4

69.9

60.2

ø30

ø41

ø38.5

ø41

ø38.5

39.6

39.4

48.6

62.6

51.2

48.6

28(23.1)

27.8(23.2)

ø30 ø30

29.5

60.9

41

65

75.5

Mounting position
(inside)

117.8

129.2

137

EP

23.5

30

Weight: 580g

Unit: mm

Unit: mm

Eyepiece Magnification 10✕, erect image (inverted when

used with erect image observation tube),
F.N. 14. Diopter adjustment range: ±5 1/m.
Provided with rubber eye shade.

Measuring scale Scale lines graduated in increments of 1mm in

the entire 10mm length. Shift of scale lines: 1mm
per rotation of the shift ring, the circumference of
which is divided into 100 graduations.

Measuring range 10mm/objective lens magnification

±5% by combined use of the zoom
compensation ring and the provided stage
micrometer. Compensation ring clamping screw.
Magnification compensation scale.

Actual size

Actual size (mm) =

Measured value (mm)

Objective lens magnification

Repeatability

Repeatability error ±

0.007
mm

A

(A …Objective lens magnification)

Accuracy *Measuring error

(A …Objective lens magnification:

L …Measured length in mm)

±[ (0.0002✕A+0.002) L +

0.007
] mm

A

Compensation
limit for objective
lens magnification
tolerance

*EP=eyepoint

WHN10x 22 — 24 90
WHN10x-H 22 -8 — +5 24 170 With adjustable diopter
CROSSWHN10x 22 -8 — +5 — 170

With cross lines and adjustable diopter
WH15x 14 — 24 90
SWH10x-H 26.5 -8 — +2 — 210 With adjustable diopter
MICROSWH10x 26.5 -8 — +2 — 210

With micrometer and adjustable diopter
CROSSSWH10x 26.5 -8 — +2 — 210

With cross lines and adjustable diopter

Field

Number

Diopter

adjustment range

(1/m)

Micrometer

diameter (mm)

Weight

(g)

Name Remarks

REVOLVING NOSEPIECES

U-P4RE

Centerable quadruple revolving nosepiece with

slider slot for DIC

U-P6RE

Centerable sextuple revolving nosepiece with slider slot for DIC

Weight: 1kg Weight: 1kg

104

(114.4)

38

40

48.2

76.4

ø102.4

116.5

40

47.2

(125.6)

38 87.6

ø116.5

34

116.5

40

ø116.5

(125.6)

38 87.6

47.2

Revolving nosepieces for BF objective lenses

Choose from following 6 types. For motorized nosepieces, refer to motorized unit page.

U-D6RE

Sextuple revolving nosepiece with slider slot for DIC

U-D6RE-ESD

Sextuple revolving nosepiece with slider slot for DIC

with ESD treatment

ø84

40.8

26.5 60.9

(87.4)

40

83

ø102.4

104

40

48.2

(114.4)

38 76.4

Weight: 520g Weight: 800g Weight: 980g

U-5RE-2

Quintuple revolving nosepiece

Unit: mm

U-D7RE

Septuple revolving nosepiece with slider slot for DIC

Insert the DIC dummy when not using the DIC slider

REVOLVING NOSEPIECES

U-D6BDRE

U-D6BDRE

35

Revolving nosepieces for BF/DF objective lenses

Choose from following 3 types. Use of adapter to mount BF objectives (BD-M-AD) enables attachment of brightfield objective
lenses. For motorized nosepieces, refer to motorized unit page.

U-5BDRE

Quintuple revolving nosepiece for BF/DF

U-D6BDRE

Sextuple revolving nosepiece for BF/DF

with slider slot for DIC/

U-P5BDRE

Centerable quintuple revolving nosepiece

BD-M-AD

Adapter to mount BF objectives

Weight: 800g Weight: 1kg

U-D5BDRE

Quintuple revolving nosepiece for BF/DF

with slider slot for DIC

Weight: 800g

Weight: 10g

Unit: mm

Insert the DIC dummy when not using the DIC slider

104

(111.2)

ø102.4

48.2

34.8 76.4

40

(114.4)

ø102.4

48.2

38 76.4

40

104

JAPAN

(125.6)

Ø116.5

47.2

38 87.6

40

U-D6BDRE

2A00002

JAPAN

116.5

W26✕0.706

W20.32✕0.706

0

+0.2

ø30

ø28.2

(4) 4

8

36

2/3" CCD

1/2" CCD

Field of view (F.N.)

Projection area

Practical field of view (mm) =

Projection area (Field Number)

Objective lens magnifications

2/3" CCD 1/2" CCD 1/3" CCD
U-TV1x-2 1x 11 8 6
U-TV0.63xC 0.63x 17.5 12.7 9.5
U-TV0.5xC-3 0.5x 22 16 12
U-TV0.35xC-2 0.35x — 22 17.1
U-TV0.25xC 0.25x ——24

]Video camera adapter

(Projection lens)

Projection

magnifications

Projection area (F.N.)

Focus the video camera adapter to prevent defocusing the eyepiece image and defocusing by magnification switching.
Generally, the video camera adapter is focused by switching to a low magnification after focusing at a high magnification.objective lens.

C-mount video camera ports

Allows direct attachment of a C mount video camera. Four types are provided: 0.63x, 0.5x, 0.35x and 0.25x.
All models feature a focus adjustment function

U-TV0.25xC

C-mount video port with 0.25x lens

U-TV0.35xC-2

C-mount video port with 0.35x lens

U-TV0.5xC-3

C-mount video port with 0.5x lens

U-TV0.63xC

C-mount video port with 0.63x lens

Weight: 1.2kg Weight: 100g

Weight: 200g Weight: 430g

Unit: mm

ø36

ø64

147.3

17.53

1-32UN

156.84

3.5

ø

60

Image plane

1-32UN

ø60

17.53

12.4

4

22.4

Image plane

ø60

17.53

ø30

3.5

32.6

30.1

42.1

1-32UN

Image plane

78.25

68.75 3.5

ø30

17.53

Image plane

1002A0 0

ø60

1-32UN

VIDEO CAMERA ADAPTERS

Video camera port

This port can be attached directly to the trinocular observation tube as well as to the single port tube with lens.

VIDEO CAMERA ADAPTERS

37

U-TV1x-2

Video port 1x

Video camera mount adapters

Allows attachment to video cameras with C, Bayonet, Sony and F mounts.
Use with the U-TV1x-2. Focus by amount of screwing into U-TV1x-2.

Unit: mm

Unit: mm

U-CMAD3

C-mount adapter

U-BMAD

Bayonet mount adapter

U-TMAD

T mount adapter

U-FMT

F/T mount adapter *

U-SMAD

Sony mount adapter

Weight: 70g Weight: 30g

* It must be combined with U-TMAD

Image plane

Image

plane

M56X2

49

ø42

ø64

48

30

3

ø64

ø48

60

38

40 4

Image plane

M56X2

17.53

M56X2

1-32UN

ø30

ø44.5

ø64.4

80.5

60.5

48.7

20

4

Image plane

4

23

43

ø64

ø42

M42X0.75

M56X2

55

ø54.7

ø45.7

13

46.5

Image plane

Weight: 165g Weight: 80g Weight: 90g

Weight: 150g

ø64

22

28

ø60

MOTORIZED UNITS

38

(169)

844045

41

87

(487)

400

64.8

81.5

211.5

86.2

115

80

108

11.8

12

107

Illuminator cable length: 1.800mm Weight: 5.5kg(exclude objective lens)

Illuminator cable length: 1.800mm Weight: 4.3kg

BX-RLAA+U-D6REMC+U-LH100-3

Motorized BF/DF reflected light illuminator+motorized Nomarski DIC sextuple revolving nosepiece+100W halogen lamp housing

Enables motorized exchange of objective lenses, selection between brightfield and darkfield observations as well as aperture diaphragm
closing/opening. The BX-UCB control unit has an RS232C connector, allowing control via a PC.
For method of attaching illuminator, refer to page 24.

BX-RFAA

Motorized universal reflected light illuminator

Reflected light fluorescence illuminator with simultaneous attachment of six mirror units. Incorporates motorized mirror unit changeover and shutter.

Motorized units

Various motorized units, perfect for automation of equipment, are available.

Unit: mm

15

88

ø84

126

109.5

9276

87.5
41

11.6 17

86.8 27.2

135

371

261

11.6 12

41

14

Revolving
nosepiece
mounting
position

Illuminator
mounting
position

MOTORIZED UNITS

39

68.3

190.5

115

U-D5BDREMC

Motorized quintuple BD revolving nosepiece with slider slot for DIC

U-D6REMC

Motorized sextuple revolving nosepiece with slider slot for DIC

U-P5REMC

Motorized centerable quintuple revolving nosepiece with slider slot for DIC

146

32

7°

105

108

332 (depth)

310

125

212

216

* Extension cord U-RMT (1700mm) should be used to connect the lamp housing (U-LH100-3) to the BX-UCB.

U-HSTR2

Hand switch

Weight: 1.0kg

Cable length 2000mm Weight: 370g

BX-UCB

Control unit

Motorized units including motorized illuminator and auto focus unit can be totally controlled
from BX-UCB

BX-REMCB

Control box for motorized nosepiece and BF/DF illuminator

BX-RLAA and U-D5BDREMC/U-D6REMC/U-P5REMC can be controlled from
U-HSTR2, or direct from the computer keyboard via an RS232C connector.

* BX-RFAA and U-D5BDREM/U-D6REM combination not applicable.

U-ACAD4515

AC adapter for BX-REMCB

Unit: mm

35±1

71±1

129.5±1

0

+100

2000

39.8
34

144

190.4

Weight: 1.1kg

MOTORIZED UNITS

40

Unit: mm

ø78

R88

147.5

108

135

170.5

148

312.5

956

58.2

58.4

14.8

62.5

71

* Consult your Olympus dealer about the motorized focus.

Cable length: 2000mm
Weight: 3.3kg

U-AFA1M

Active auto focus unit

Weight: 1.0kg

U-FWR

Motorized reflected filter wheel

Accomplish maximum 6 filter position exchange

2000

147.9

180.5

130

58.5

24.5

42

30.5

MOTORIZED UNITS

41

BXFMA-F

Motorized illumination with power focus

A motorized microscope unit for integration with your equipment. Motorized operations such as revolving nosepiece up/down, objective lens
switching, aperture diaphragm open/close, and brightfield/darkfield switching are accomplished with this component.
Several microscopic operations are totally controlled from an external unit by combining this component with an auto focus unit.

U-FH

Focus adjustment knob unit

U-IFFH

Focus adjustment knob interface

Unit: mm

Weight: 7.6kg

Weight: 1450g

Weight: 760g

* Consult your Olympus dealer about the mounting dimensions.

77

206

310.5

5656169

5177

75.5

5470 4

82.3

133

92

334

341.6

70

33.6

91.5

50

210
214

3

100

104

DEEP ULTRAVIOLET OBSERVATION SYSTEM

42

Deep ultraviolet observation system

This module adds a deep ultraviolet (248nm) optical system to a general microscope.
An ultra-high resolution observation is executed by using an extremely short wavelength ray.

Unit: mm

108

258.5

69

79

ø8 (light guide)

3430.4

105.8

2000

+200
0

or 5000

+200
0

(39.5)6 5.5

ø70

196.8

196

ø30

ø39.4

1-32 UNF
(C Mount Thread)

45

(distance between

mounting positions)

102.5

U-UVF248IM

UV248 compatible intermediate tube

U-UVF2FB/5FB

UV quartz light guide

U-UVF248LB+U-LH80HGXE

UV248 compatible light source box + Mercury Xenon lamp housing

8170

227

41240

210.3

4-ø4.5, ø8 C'bore 5 Deep

4-ø4.5, ø13 C'bore 5 Deep

150

15045
18030

170

157.5

20

Weight: 1.9kg

Weight:6.5kg

Weight: U-UVF2FB 50g

U-UVF5FB 80g

1. Field Number (F.N.) and Practical Field of View

The field number (F.N.) is referred to as the diaphragm size of
eyepiece in mm unit which defines the image area of specimen.
The diaphragm diameter actually seen through eyepiece is
known as the practical field of view (F.O.V.) which is determined
by the formula:

F.O.V. =

Eyepiece F.N.

(mm)

Objective lens magnification

2. Working Distance (W.D.)

The distance between the front edge of the objective lens and
the specimen surface (with the surface of the cover glass in case
of the cover glass objective lens) when the specimen is focused.

3. Parfocal Distance

It is the distance between the objective lens mounting plane and
the specimen. In UIS2/UIS objective lenses, the parfocal
distance is designed at 45mm.

For parfocal distance of the LCPLFLN-LCD series objective
lenses, refer to the appropriate objective lens page.

4. Relationship between the objective lens's focal length
and magnifications

Indicated magnifications of UIS2/UIS objective lenses are the
values when the focal length of the tube lens is 180 mm.

M

(ob)=

Focal length of tube lens

f

M(ob): Objective lens magnification

f: Objective lens's focal length

5. Total Magnification

5.1 Observation through eyepiece (binocular observation)

M(bino)=

M

(ob)×

M

(oc)

M(bino): Total magnification for binocular observation
M(ob): Objective lens magnification
M(oc)

: Eyepiece magnification

5.2 Video monitor observation

● Total magnification for video monitor

M(video monitor)=

M

(ob)×

M

(

video camera adapter

)×Monitor magnification*

M(video monitor)

: Total magnification on the video monitor

M(ob)

: Objective lens magnification

M(video camera adapter): Projected magnification for video camera

adapter including photo eyepiece

(refer to Figure 1)

* Refer to Figure 3 for "Monitor magnification"

● Practical field of view for video monitor observation

M

(ob): Objective lens magnification

M

(video camera adapter): Projected magnification for video camera

adapter including photo eyepiece

(refer to Figure 1 for projected magnifications)

* Refer to Figure 2 for image device size

Example

What is total magnifications for video monitor when objective
lens is 50x, video camera adapter U-TV0.5xC and 2/3" video
camera are used ?

OPTICAL TERMINOLOGY

43

Working distance and parfocal distance

Working Distance (W.D.)

Parfocal distance

Objective lens
mounting position

Focal plane

Figure 1 Video camera adapter and projection magnifications

Video camera adapter (Projection lens)

Projection magnifications

U-TV1x-1 + 1x
video camera mount adapters

U-TV0.63xC 0.63x
U-TV0.5xC-3 0.5x
U-TV0.35xC-2 0.35x
U-TV0.25xC 0.25x

Figure 2 Imaging device size

Camera format Diagonal Horizontal Vertical

1/3" 6.0mm 4.8mm 3.6mm
1/2" 8.0mm 6.4mm 4.8mm
2/3" 11.0mm 8.8mm 6.6mm

The above table is for standard image device sizes.
Check your device size for precise calculation.

Practical field of view for

=

Image device size *

video monitor observation

M(ob)×M(video camera adapter)

Figure 3 Imaging device size and monitor magnifications

Camera format

Monitor size (diagonal)

9" 12" 14" 21" 27"
1/3" 38.1x 50.8x 59.2x 84.6x 114.1x
1/2" 28.6x 38.1x 44.5x 63.5x 85.7x
2/3" 20.8x 27.7x 32.3x 46.2x 62.3x

•Total magnification on the video monitor:

m(ob)=50×, M(video camera adapter) is 0.5× from Figure 1 and monitor

magnification is 46.2× from Figure 3.

M(monitor observation)=M(ob)×M(

video camera adapter

)×monitor magnification

=50×0.5×46.2=1155×

•Practical filed of view for video observation(horizontal side):

M(ob)=50×, M(video camera adapter) is 0.5× from Figure 1 and

horizontal side of 2/3" imaging device is 8.8mm from Figure 2

=

8.8 (mm)
=352µm

50 × 0.5

6. Numerical Aperture (N.A.)

The numerical aperture is a key factor to the performance of
objective lens (resolving power, focal depth and brightness).
The N.A. is determined by the following formula:

N.A.= n × sinθ

n=Refraction rate of the medium between specimen and

objective lenses. (Air: n=1, oil: n=1.515)

θ: Angle which is made by the optical axis and refraction of the

light farthest from the center of the lens.

The visual field brightness (B) of the microscope is determined
by the following formula in relation to the objective lens
magnification (M). The larger the N.A. and the lower the
objective magnification, brightness will increase in the factor of
the second power.

B

∝

N.A.

2

M

2

7. Resolving Power

The resolving power of an objective lens is measured by its
ability to differentiate two lines or points in an object. The greater
the resolving power, the smaller the minimum distance between
two lines or points that can still be distinguished. The larger the
N.A., the higher the resolving power.

● Resolving power formula

The following formula is generally used for determing resolution.

ε = 0.61 ×

λ

(

Reyleigh formula)

N.A.

λ: Wavelength or radiation in use

(λ=0.55µm is used for visible light)

N.A.: Objective lens N.A.

Example

MPLFLN100×(N.A.=0.90), λ=0.55µm

ε = 0.61 ×

λ =0.3355

=

0.3355
= 0.37µm

N.A. N.A. 0.90

8. Focal depth of Microscope

The focal depth refers to the depth of the specimen layer which
is in sharp focus at the same time, even if the distance between
the objective lens and the specimen plane is changed when
observing and shooting the specimen plane by microscope. As
human eyes are individually different in the ability of their focus
adjustment, each person's perception of the focal depth varies.
At present, the Berek formula is generally used, because it gives
a focal depth value that often coincides with that obtained
through experiments.

Focal depth formula

● Visual observation (Berek formula)

± D.O.F.=

ω× 250,000

+

λ

(µm)

N.A. × M 2(N.A.)

2

D.O.F.: Depth Of Focus

ω: Resolving power of eyes 0.0014

(when optical angle is 0.5 degrees)

M: Total magnification

(objective lens magnification x eyepiece magnification)

➔

± D.O.F. =

350 +0.275

(λ=0.55µm)

N.A. × M N.A.

2

This indicates that the focal depth becomes smaller as the
numerical aperture becomes larger.

Example

With MPLFLN100×(N.A.=0.90), WHN10×:

± D.O.F. =

350 +0.275

= 0.39 + 0.34 = 0.73µm

0.90 × 1,000 0.81

● Video camera

In the case of a video camera, the focal depth will vary according
to number of pixels of CCD, optical magnification, and numerical
aperture. The above-mentioned formula is used as a rough
guide only.

OPTICAL TERMINOLOGY

44

Objective

Numerical aperture

Sample
surface

n=1

(air)

θ

Practical field of view

=

Image device size

for video observation

M

(ob)

× M

(video camera adapter)

9. Aberrations

A difference between an ideal image and an actual image that
passes through an optical system is called an “aberration.”

9.1 Requirements for Ideal Image Formation

The following three requirements must be satisfied to form an
image with no aberration, or an ideal image.

(i) All the light rays coming from a single point and passing

through an image formation optical system converge on a
single point.

(ii) Image points, which correspond to object points on the

same plane perpendicular to the optical axis, are present
on the same plane.

(iii) The planar shape of an object and the planar shape of an

image that are on the same plane perpendicular to the
optical axis have a similarity relation.

In an actual optical system, however, it is very difficult to strictly
meet the requirements for ideal image formation and this causes
“aberrations” that interfere with image forming performance.

9.2 Classification of Aberrations

Aberrations that interfere with image forming performance are
classified as shown below in Figure 9-2.

Seidel’s aberration = “Expansion of a point image” +
“Curvature of image plane” + “Deformation”

Types (1) to (3) correspond to “expansion of a point image” that
goes against requirement (i) for ideal image formation in Figure 9-

1. Type (4) corresponds to “curvature of image plane” that goes
against requirement (ii) in Figure 9-1. Type (5) corresponds to
“deformation” that goes against requirement (iii) in Figure 9-1.
Types (6) and (7) correspond to “color blur” of images caused by

characteristics of glass materials used for the optical system.

“Expansion of a point image” can also be expressed by
“wavefront aberration” that regards the light as “waves” and

takes account of the phase to include the influence of diffraction.

(1) Spherical aberration

When light rays coming out of an axial object point enter a lens,
the light rays with a larger numerical aperture (N.A.) are
subjected to stronger refraction power and cross the optical axis
in positions with larger differences from the ideal image
formation position. The aberration caused this way by different
image forming positions due to differences in N.A. of axial light
rays is called “spherical aberration.” (“Spherical aberration” is
proportional to the cube of N.A.)

It is said that objective lenses with larger N.A. have better
resolution but worsen spherical aberration. Our advanced
design and manufacturing techniques have realized good optical
performance even with large numerical aperture.

(2) Coma aberration

Even though spherical aberration is compensated to be very
small, there are cases where light rays coming out of an off-axis
object point are not condensed to a single point on the image
plane but generate asymmetric blur just like a comet leaving
traces. This is called coma aberration.

(3) Astigmatism

Even though a lens is compensated for spherical aberration and
coma aberration, there are cases where an image of an off-axis
object point is not focused to a single point but separated to a
concentric line image and a radial line image. This is called
“astigmatism.” When astigmatism is present, a point image
blurs vertically and horizontally, before and after the focus
position.

45

OPTICAL TERMINOLOGY

Object

（i）（ii）（iii）

Figure 9-1 Requirements for Ideal Image Formation

Image plane

Figure 9-3 Spherical Aberration

Specimen

Objective lens with
spherical aberration

Aplanatic
tube lens

Image plane

Figure 9-2 Classification of Aberrations

Seidel's

aberration

Aberration

Chromatic

aberration

(1) Spherical aberration
(2) Coma aberration
(3) Astigmatism
(4) Field curvature
(5) Distortion

(6) Longitudinal (axial)
chromatic aberration

(7) Chromatic aberration
of magnification

Figure 9-4 Coma Aberration and Spot Shape
on the Image Plane

Specimen Aplanatic

Objective lens with
coma aberration

tube lens

Image plane

(4) Field curvature

An image plane of an object on a plane perpendicular to an
optical axis does not always become a plane perpendicular to
the optical axis, but it generally becomes a curved plane. This
symptom is called “field curvature.”
When field curvature is present, the image is more displaced as
it becomes closer to the periphery of the visual field. Therefore,
when the center of an image is brought into focus, blur occurs in
the peripheral areas of the image. To bring the entire image,
including the periphery, into clear focus, it is necessary to
adequately compensate for this type of aberration.

(5) Distortion

When there is no similar relation between a planar shape on an
object and a shape on the image plane, this is called “distortion.”
When distortion is present, a square image appears in a shape
of a barrel or pin-cushion as shown in Figure 9-6.

The microscope optical system contains some distortion. When
distortion is present, it can bring erroneous results of shape
measurements. When a microscope is used for precision
measurements, pay close attention to this aberration, for
example, by providing it with an aberration compensation
function.

(6) Chromatic aberration

Glasses used for optical systems have different refractive
indexes depending on the wavelength. This causes differences
in focal length between wavelengths and generates
displacement of image forming position. This phenomenon is
called “chromatic aberration,” which is sometimes subdivided
into axial displacement on the optical axis, called “axial
chromatic aberration” (or lateral chromatic aberration) and
displacement on the image plane, called “chromatic

aberration of magnitude.”
Many special glass materials are used, e.g., for apochromats
(MPlanApo in Olympus), to eliminate chromatic aberration in a
wide range from violet light (g-rays with wavelength of 435 nm)
to red light (c-rays with wavelength of 656 nm).

9.3 Wavefront Aberration

Since a long time ago, aberrations have been used in “geometric
optics,” which considers light as “light rays.” Microscope optical
systems are often used for observation of very small specimens
at a wavelength level, and sometimes adopt “wave optics,”
which regards light as “waves” and handles the phase
information, taking account of the influence of diffraction.
In such a case, “wavefront aberration” is used for evaluation.
As shown below, when requirements for ideal imaging are
satisfied in a microscope optical system, the spherical wavefront
(spherical waves) coming from a single point on an object
(specimen) is converted to plane waves through an ideal
objective lens. The plane waves are converted to spherical
waves through an ideal tube lens, and condensed to a single
point. The wavefront of these waves is called the “ideal
wavefront.”

Based on the figure indicated for (1) spherical aberration, the
behavior of the wavefront in an optical system that has an
aberration is described below.

A difference (a degree of disagreement) between the ideal
wavefront and the actual wavefront shown above is called
“wavefront aberration.”

46

OPTICAL TERMINOLOGY

Figure 9-5 Astigmatism and Change in Spot
Shape in Different Focus Positions

(a) (b) (c)

(a) (b) (c)

Figure 9-7 Ideal Microscope Optical System

Specimen Ideal

Spherical

Spherical

wave

wave

Ideal
objective
lens

Plane

Plane
wave

wave

tube lens

Spherical

wave

Image plane

Figure 9-6 Distortion

(a) Barrel shape
type

(a) Pin-cushion
type

Figure 9-8 Illustration of Wavefront Aberration

Actual

Specimen

Objective lens with

Objective lens with
spherical aberration

spherical aberration

wavefront

Ideal

wavefront

Specifications are subject to change without any obligation on the part of the manufacturer.

Printed in Japan M1606E-1106B

•OLYMPUS CORPORATION has obtained ISO9001/14001.