22
MIC-D
Software
I.ooo-
Image Adjust
rn
ent and Manipulation
Most images that you take willrequire verylittle if any adjustment. Remember to e nter
the White Balance Number (WB#) from the base of the microscope to maximize color
rendition. Some important features to note and understand in the Image
Process
ing
Win
dow
are:
White and
Black
Balance
Adjusting the White and Black Balance ensures that corresponding areas of the image
are correctly interpreted by the softwa re, so that white areas are 'seen' as being white
and 'blacks ' as black. To perform a White Balance
ope
ration, select an area of the image
that you think should be white. This is the region you will te
ll
the processing software it
should interpret as being white in case it is tinted. If this area is very sma
ll
(just a few
pixe
ls)
then use the 'Point' sampling tool, which willenable you to samp le a very sma
ll
area or point. If the region is larger, use the 'Area' tool to draw a box over a'white' part
of the image. Next, click on the 'Select White Balance' button and draw a box over an
area of the image with the cursor that you think ought to appear white. A single click of
the mouse over a white area is sufficient if you are using the 'Point' sampling tool. Next,
click on 'Apply White Balance' . Note the slight difference in the image. Carry out the
corresponding Black Balance adjustment, if you think that there are re
gi
ons of the image
that ought to
appea
r black.
Digital VersusOptic
al
Zo
omi
ng
The Digital Zoom is useful for zooming in on a captured image. It is important to note
that digital zooming is quite different from
opt
ical zooming (performed with the MIC-D
Microscope itsel
f.)
When you zoo m digitally, you are merely enla
rgi
ng a fixed number
of pixelsso
that
they are spread over a larger space on the comp uter monitor or in print.
Detail is not
added
to the image.Existing detailis merely expanded. So, a ten pixel by
ten pixel digital image (
100
pixel area) that is blown up two times will still only contain
one hundred pixelsworth of image information. W hen an O ptical Zoom isinc
rease
d in
power, the light recieved from the actual specimen or sample (containing detail that
could not be detected at a lower magnification beca
use
it was too small or too fine) is
revealed. An area of a specimen that spans a
100
pixelsarea in the Live Image Window
wi
ll
be see n by four times that number of pixelsafter two times
opt
ical zooming, with
four times as much visible detail revealed.
Digital ZoomO ptical Zoom
•
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Vs.
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