Olympus MIC-D Quick Start Manual

OLYMPUS
'
Quick Start Guide
Learn
more about your MIC-D, down-load free software and explore
vast image libraries at the Olympus MIC-D website.
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Detailed software installation instructions may also be found at
the
MIC-D Website
Quick
Start
Guide
In the lab or in the field, the Olympus M I
C-D
por
table
digita
l microscope captures still and moving images instantly. This Quick Start Guide will int
roduce you
to
the MIC-D's i
nnovat
ive design and spectacular imaging
capab ilities, so
yo
u'll be up and taking p
ictur
es in no ti me.
MIC-D Pa
rts
Explained
MIC-D
Software
Introduction
Choos
ingYour Specimen
MIC-D
Basic
Controls Illumination Technique Image Capture Window
Still Imagesand Mov
ies Time LapseMovies Sav
ing Images
Library Window
- ViewingCaptured Images
Image
Process
ingWindow
Line Drawingand Annotation Image Manipulation and Adjustme
nts
Polarized Light Kit
2-3
4-5
6
7
B-9
10-11
12-13
14
15-1B
19
20
21-22
23
2
Get
To
Know
The
M C-D
Lamp
Head
"-
Light Diffuser~
Stage
'\.
Optical Zoom Control
'\.
Magnification
Indicator
Base
Tilting
Lamp
Arm
/
(
Focus
Control
/
Light Intensity
Control
/
3
....._ Lamp
/;I
ead
~~
The Lamp Head contains an extreme ly bright whiteL.E.D. This device is brighte r than an ordinary bulb and uses less power. The Lamp Head rotates slightly to allow fine tuning to the illumination angle. Make sure th
at
it is rotated all the way clockwise for Transmitt
ed
Light work (Brightfield) and all
the
way
co
unter clockwise for ReflectedLight work.
....._Light Diffuser
__
TheLight Diffuser ca n be pushed in and pulled out. Keep the Diffuser allthe way in
during Transmitted
Li
ght Illumination, and allthe way out for Reflected
Li
ght
Ill
umination . Vary as ne
eded
for Oblique and Darkfield settings.
....._I ilting Lamp Arm
Adjustments to the angle of the L
amp
Arm are the key to the different illumination tec hniques. Use the live image to guide adjustments. The Arm should be set to the vertical position for Transmitted
Lig
ht, to 11 o'clock for
Ob
lique
Ill
umination
andtoabout
10:30 forDarkfield . The arm should be rotated all the way counter clockwise for the
Reflect
edLight Setting.
......
Gliding
Stage
__
The Glid ing Stage is
the
platform to place specimens or samples for observation. The MIC-
D's Stage glides 5mm in any horizontal direction,
and
can be ro
tat
ed 360 degrees. Use
the Gliding Stage to adjust the specimen position. Reme
mbe
r t
hat
up, down and rotational
movements are inverted.
_
-.Lo
oom Control
The MIC-D boasts an all glass optical
zoo
m lens to enable continuous magnification
changes. The magnification range is an amazing 22x to 255x.
_
-I
'vlagnification Indicator
Use
the
Magnification Scale to read the zoom
pow
er, by aligning
the
scale markings on
the blue
zoom
handle to the
spot
below. Adjust the Magnification Slide Bar in
the
Image
C
apture
software window to match the setting on
the
MIC-D, in ord er to calibrate
the
scale mark ing in
the
live image window and captured image.
....._Light
Intensity.
Control
Li
ght intensity (and focus) should be initially set with the
zoomat
minimum powerto
preve nt und er exposure. The
MI
C-D's expos ure setting will then be auto
mat
ically
adjuste d to maintain consiste nt brightness in
the
live image. For Reflected
Li
ght
Illumin
at
ion turn
the
Lig
ht Intensity Control to maximum.
....._F.ocusControl
Rotate this knob to bring specime ns and sam ples into clea r focus. The MIC-D's lens can focus on surfaces up to 5mm above the stage.
4
MIC-D
Software
Image
Capture
Window
.
-
.-
Library
Window
Image
Processing
Window
ki
l
5
MIC-D
Software
The MIC-D Software enables you to view, capture, process and annotate
images in
three
simple windows.
Io-_Image
Capture
Window
Capture and save still and moving images viewed in
the
Live
Image Window with
the
simple click of a mouse button.
You
can
view all of your captured images in
the
tile bar below
the
live
window. The scale bar, light intensity and image format may also
be adjusted here .
Io-_Librar.y.
Window
The Library Window
is
where
you can view your captured
images as thumbnails or in
full
size. Still images may be
displayed side by side for compar ison purposes. Moving images
may also viewed here.
Io-_Image
P-
rocessing
Window
Annotation, color and brightness adjustments are performed in
this window. Digital zooming as well as white and black
balancing functions are available for selection.
6
Choose
Your
5
ecimen
or
Sam
~le
With
the
MIC-D'sinv
erted
lens
design,
you
can
image
just about any sample,
regardless
of
its
shape orwhether
light
can
pass
through
it-
thanks
to the
Reflected
Light
capacity.
Micros
cope
slide
preparations and thin, translucent specimens can
be
viewed
in
Brightfield
, Oblique or
Darkfield.Use
Oblique
Illumination
to
highl
ight
structural details and
Darkfield
for
low
contrast specimens. Oblique
Illumination
will
shade
rises
and depressionsinyour
specimen or sample.
Darkfield
can be
used
to emphasize
color.Use
Refle
cted
Light
for
anything
large
and opaque.
Don't
forget
that
for
field
work
you
can
use
the
MIC-D
anywhere
you
can take a
laptop computer.
Transmitt
ed
Light
!Brightfield!
Oarkfield
Reflected Light
Obliq
ue
Illumin
ation
7
MIC-D
Basic
Controls
Let's
start
by
getting
familiar
with
the
basic
hardware
controls.Keep
an
eye onthe
Live
Image
Window
as
you
manipulate the
MIC-D
controls
.
Make
sure
that the
Light
Diffuserispushed
in,
the
Illumination
Armisin
the
vertical
position
and the
lamp
head
is
rotated
fully
clockwise.Turn
the
MIC-D's
light
source on and
increase
the
light
intensity
by
turning the
control
clockwise,
about one
half
as
far
as
it
will
go.The
MIC-D
will
automatically
make
adjustments to the
light
intensity
afteritis
initially
set.
Turn
the
Optical
Zoom
Control
to a
minimum
setting.
The
Optical
Zoom
Control
enables the
user
to
physically
adjust the
position
of the
microscope's
lens
to obtain
magnification
powers
from
22x
to
255x!
Bring
the
specimen
into
focus
in
the
Live
Image
Window
by
rotating
the
Focus
Control
Knob.
Make
any
final
adjustments to the
light
intensity.
Notice
that the
Gliding
Stage
Moves
freely
in
any
direction.
Moving
the
Gliding
Stage
instead
ofthe specimen or
sample
will
allow
you
to
scroll
the
image
smoothly,
even
at
high
magnification.When
you
have
found
an interesting deta
il,
center it
in
the
Live
Image
Window
and
zoom
in.
Continue to
make
adjustments to the
focus
as
needed.
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