ISS-UV/VIS
Integrated Sampling System
Installation and Operation Instructions
Description
The ISS-UV-VIS Integrated Sampling System is a combination of a RF deuterium source with a
tungsten halogen bulb connected to a cuvette holder for 1-cm cuvettes. The cuvette holder attaches
directly to the light source and has a 5-mm diameter f/2 collimating lens to collect the light. The ISSUV-VIS can be operated manually or through the spectrometer operating software.
Parts Included
The ISS-UV/VIS Integrated Sampling System ships with the following components:
ISS-UV-VIS Deuterium Tungsten Light Source/Cuvette Holder
Power cord for connecting the ISS-UV/VIS to outlet
15-pin accessory cable for software control of the ISS-UV/VIS
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ISS-UV/VIS Installation and Operation Instructions
Installing the Sampling System
Do not attempt to connect the ISS-UV/VIS to the spectrometer without following the installation
instructions in this section. Furthermore, ensure that the spectrometer operating software (OOIBase32
or SpectraSuite) is installed on your PC and that the spectrometer is obtaining data before connecting
the ISS-UV/VIS.
WARNINGS
The light beam emitted from the ISS-UV/VIS contains ultraviolet
radiation and can cause eye damage. Never look directly into the light
source and always use safety goggles when working with this product.
Dangerous voltage levels are present, and there are no user-
serviceable parts. Do not open the unit.
For any experiments using UV light, we recommend using our
solarization-resistant fiber.
Contact Ocean Optics for bulb replacement.
Do not use this instrument for any clinical or diagnostic
measurements.
Dropping the unit can cause permanent damage. Handle with care.
Adjusting the Fit of the Cuvette
The ISS-UV-VIS is designed to hold 1-cm square cuvettes. When properly adjusted, the cuvette
should fit snugly into the holder.
► Procedure
1. Locate the two ball plunger screws.
2. Use a small flathead screwdriver to loosen the two ball plunger screws until the end of each
screw is visible in the holder.
3. Insert a cuvette into the cuvette holder.
4. Tighten the ball plunger screws until the cuvette is stationary in the cuvette holder. Do not
over-tighten.
5. Remove the cuvette from the cuvette holder.
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ISS-UV/VIS Installation and Operation Instructions
Connecting to the Spectrometer
► Procedure
1. Remove the power from the spectrometer by disconnecting it from the PC or detaching the
USB cable.
2. Attach an optical fiber to the SMA connector on the front of the ISS-UV-VIS and attach the
other end of this fiber to the SMA connector on your spectrometer.
3. Plug the wall transformer into a standard 110 V outlet. Plug the 12 V output into the back of
your ISS-UV-VIS above the
the transformer into a standard 220 V outlet. At this time, the green LED indicator light on the
front of the lamp will be lit. This indicator light only means that the lamp is receiving power,
not that the deuterium and tungsten bulbs are on.
12V label. For users of European-version wall transformers, plug
Operating the ISS-UV-VIS Manually
► Procedure
1. Find the switch on the back of the ISS-UV-VIS.
2. There are three positions:
On position. There can be up to a 1.5 second delay between switching the lamp to on and the
bulbs igniting. If the lamp has not been used recently, the deuterium bulb may take up to 60
seconds to ignite.
3. For 0.3% peak-to-peak stability, allow 30 minutes warm-up time before taking your
measurements.
On, Off, and Remote. For Manual operation, move the switch to the
Operating the ISS-UV-VIS Through Software
► Procedure
1. Take the 15-pin accessory cable and plug one end into the spectrometer. Connect the other end
of the accessory cable into the back of the ISS-UV-VIS.
2. Find the switch on the back of the ISS-UV-VIS. There are three positions:
For Software operation, move the switch to the
Remote position enables you to control the lamp through the software, whether you are using
OOIChem, OOIBase32, or SpectraSuite.
3. Start the operating software.
For SpectraSuite software, check the Strobe/Lamp Enable check box on the Acquisition
toolbar to turn the light source in the ISS-UV-VIS on. Uncheck this box to turn the light
source off.
For OOIBase32 software, select Strobe Enable from the OOIBase32 Acquisition
Parameters menu bar.
For OOIChem software, select Spectrometer | Enable Strobe from the menu.
Remote position. Moving the switch to the
On, Off, and Remote.
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ISS-UV/VIS Installation and Operation Instructions
There can be up to a 1.5 second delay between turning the bulbs on via the software and the
bulbs igniting. If the lamp has not been used recently, the deuterium bulb may take up to 60
seconds
3. For 0.3% peak-to-peak stability, allow 30 minutes warm-up time before taking your
measurements.
Disabling the Tungsten or Deuterium Bulb
It is possible to disable the deuterium or the tungsten bulb in the ISS-UV-VIS. Both bulbs are enabled
at the time of manufacture. In order to disable the deuterium or tungsten bulb, you must remove the
casing of the ISS-UV-VIS.
Disabling the Deuterium Bulb
It is possible to enable or disable the deuterium lamp. Jumper block JA of the ISS-UV-VIS's circuit
board controls the deuterium bulb.
Short pins 2-3 (that is, place a jumper over pins 2-3 of JA) to enable the deuterium bulb.
Short the pins 1-2 (that is, place a jumper over pins 1-2 of JA) to disable the deuterium bulb.
Disabling the Tungsten Bulb
It is possible to enable or disable the tungsten lamp. Jumper block JB of the ISS-UV-VIS's circuit
board controls the tungsten bulb.
Short pins 2-3 (that is, place a jumper over pins 2-3 of JB) to enable the tungsten bulb.
Short pins 1-2 (that is, place a jumper over pins 1-2 of JB) to disable the tungsten bulb.
Using Solarization-resistant Fibers
Solarization is the loss of color or transparency in glass due to exposure to ultraviolet radiation. If you
are using a UV light source, the UV radiation degrades the silica in a standard patch cord fiber over
time, resulting in increased overall absorption values and invalid data. This degradation is called
solarization.
Our 300-µm Diameter Solarization-resistant Optical Fiber consists of a silica core, surrounded by a
silica cladding material. The fiber is then coated in aluminum. Our solarization-resistant fibers are best
used for regions below 250 nm, or for applications where exposure to long-term UV light occurs.
Replacing the Bulbs
The deuterium and tungsten bulbs cannot be replaced by unauthorized personal. To replace a bulb in
the ISS-UV-VIS, contact Ocean Optics.
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ISS-UV/VIS Installation and Operation Instructions
Specifications
Deuterium wavelength range: 200-400 nm
Tungsten wavelength range: 360-850 nm
Deuterium bulb life: 800 hours
Tungsten bulb life: 2,000 hours
Ignition delay: ~1.5 seconds to 60 seconds
Peak-to-peak stability:
Connector: SMA 905
Power input: 12 V
Power requirement: 12 VDC/420 mA
Power consumption: 5 watts (deuterium 3.8 watts, tungsten 1.2 watts)
0.3% in 30 minutes
1.0% in 10 minutes
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ISS-UV/VIS Installation and Operation Instructions
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