Ocean Optics HR2000+ User Manual

HR2000+ High-speed Fiber Optic Spectrometer

Installation and Operation Manual

Document Number 294-00000-000-02-201012

Offices: Ocean Optics, Inc. World Headquarters

830 Douglas Ave., Dunedin, FL, USA 34698

Phone [phone]
Fax [phone]

8 a.m.– 8 p.m. (Mon-Thu), 8 a.m.– 6 p.m. (Fri) EST

E-mail: [e-mail] (General sales inquiries)

[e-mail] (Questions about orders)
[e-mail] (Technical support)

000-00000-000-02-A

Additional
Offices:

Regional Headquarters

Maybachstrasse 11
73760 Ostfildern

Phone [phone]
Fax [phone]
E-Mail [e-mail]

Ocean Optics Asia

666 Gubei Road, Kirin Tower, Suite 601B, Changning District, Shanghai,
PRC. 200336

Phone [phone]

Fax [phone]
E-Mail [e-mail]

Ocean Optics EMEA

Sales and Support Center
Geograaf 24, 6921 EW DUIVEN, The Netherlands

Phone [phone]

Fax [phone]
E-Mail

[e-mail]

Copyright © 2010 Ocean Optics, Inc.

All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, by any means, electronic, mechanical,
photocopying, recording, or otherwise, without written permission from Ocean Optics, Inc.
This manual is sold as part of an order and subject to the condition that it shall not, by way of trade or otherwise, be lent, re-sold, hired out or otherwise
circulated without the prior consent of Ocean Optics, Inc. in any form of binding or cover other than that in which it is published.

Trademarks

All products and services herein are the trademarks, service marks, registered trademarks or registered service marks of their respective owners.

Limit of Liability

Every effort has been made to make this manual as complete and as accurate as possible, but no warranty or fitness is implied. The information provided is
on an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity with respect to any loss or damages arising from
the information contained in this manual.

Table of Contents

About This Manual......................................................................................................... iii

Document Purpose and Intended Audience..............................................................................iii

What’s New in this Document ................................................................................................... iii

Document Summary..................................................................................................................iii

Product-Related Documentation.................................................................................... iii

Upgrades....................................................................................................................... iv

Chapter 1: Introduction .....................................................................1

Product Overview .......................................................................................................... 1

System Requirements ................................................................................................... 2

EEPROM Utilization .................................................................................................................. 2

About SpectraSuite.................................................................................................................... 2

Sampling System Overview....................................................................................................... 2

How Sampling Works............................................................................................................2

Modular Sampling Accessories............................................................................................. 3

Interface Options ........................................................................................................... 3

Breakout Box ............................................................................................................................. 3

Shipment Components .................................................................................................. 4

Chapter 2: Installing the HR2000+....................................................5

Overview ....................................................................................................................... 5

HR2000+ Installation ..................................................................................................... 5

Configuring the HR2000+ with OOIBase32 .............................................................................. 6

Operator and Serial Number Dialog Box .............................................................................. 6

Default Spectrometer Configuration File ............................................................................... 6

Configure Hardware Screen .................................................................................................6

Spectrometer Configuration Screen......................................................................................7

Connect Spectroscopic Accessories.............................................................................. 8

External Triggering Options........................................................................................... 8

294-00000-000-02-201012 i

Table of Contents

Chapter 3: Troubleshooting..............................................................9

Overview ....................................................................................................................... 9

HR2000+ Connected to PC Prior to Software Installation .............................................. 9

Windows Operating Systems .................................................................................................... 9

Remove the Unknown Device from Windows Device Manager ...........................................10

Remove Improperly Installed Files........................................................................................10

Mac Operating Systems ............................................................................................................11

Linux Operating Systems .......................................................................................................... 11

Older Version of OOIBase32 Installed........................................................................... 11

Appendix A: Calibrating the Wavelength of the HR2000+ ..............13

Overview ....................................................................................................................... 13

About Wavelength Calibration ....................................................................................... 13

Calibrating the Spectrometer ......................................................................................... 14

Preparing for Calibration............................................................................................................ 14

Calibrating the Wavelength of the Spectrometer ......................................................................14

Saving the New Calibration Coefficients: USB Mode ..................................................... 16

Saving the New Calibration Coefficients: Serial Mode ................................................... 17

Appendix B: Specifications...............................................................19

Overview ....................................................................................................................... 19

How the HR2000+ Works .............................................................................................. 19

HR2000+ Components Table .................................................................................................... 20

HR2000+ Specifications ................................................................................................ 21

CCD Detector Specifications ..................................................................................................... 21

HR2000+ Spectrometer............................................................................................................. 21

System Compatibility ..................................................................................................... 22

Compatibility for Desktop or Notebook PCs .............................................................................. 22

30-Pin Accessory Connector Pinout .............................................................................. 23

30-Pin Accessory Connector Pinout Diagram ........................................................................... 23

30-Pin Accessory Connector – Pin Definitions and Descriptions.............................................. 23

30-Pin J2 Accessory Connector - Part Numbers.......................................................................25

HR2000+ 15-Pin Accessory Cable Pinout ..................................................................... 26

Index ................................................................................................... 27

ii 294-00000-000-02-201012

About This Manual

Document Purpose and Intended Audience

This document provides the user of HR2000+ Spectrometer with instructions for setting up, calibrating
and performing experiments with their spectrometer.

What’s New in this Document

This version of the HR2000+ High-speed Fiber Optic Spectrometer, Installation and Operation Manual
updates information for the new triggering options for firmware versions 3.0 and above.

Document Summary

Chapter Description

Chapter 1: Introduction

Chapter 2: Installing the HR2000+

Chapter 3: Troubleshooting

Appendix A: Calibrating the

Wavelength of the HR2000+

Appendix B: Specifications

Contains descriptive information about the HR2000+
Spectrometer and how sampling works. It also provides a list
of system requirements, interface options, and shipment
components.

Provides installation and configuration instructions.

Contains recommended steps to isolate and correct common
problems.

Provides instructions for calibrating the HR2000+
Spectrometers.

Contains technical specifications and connector pinouts for the
HR2000+ Spectrometers.

Product-Related Documentation

You can access documentation for Ocean Optics products by visiting our website at

http://www.oceanoptics.com. Select Technical → Operating Instructions, then choose the appropriate

document from the available drop-down lists. Or, use the
of the web page.

Search by Model Number field at the bottom

 Detailed instructions for SpectraSuite Spectrometer Operating Software are located at:

http://www.oceanoptics.com/technical/SpectraSuite.pdf.

 Detailed instructions for the OOIBase32 Spectrometer Operating Software are located at:

http://www.oceanoptics.com/technical/ooibase32.pdf

294-00000-000-02-201012 iii

About This Manual

 Detailed instructions for the Breakout Box are located at:

http://www.oceanoptics.com/technical/HR4_breakout.pdf.

 Detailed instructions for External Triggering for firmware versions below 3.0 are located at:

http://www.oceanoptics.com/technical/external-triggering.pdf.

 Detailed instructions for External Triggering for firmware versions 3.0 and above are located at:

http://www.oceanoptics.com/technical/external-triggering2.pdf.

Engineering-level documentation is located on our website at

You can also access operating instructions for Ocean Optics products from the Software and Technical

Resources

CD that ships with the product.

Technical → Engineering Docs.

Upgrades

Occasionally, you may find that you need Ocean Optics to make a change or an upgrade to your system.
To facilitate these changes, you must first contact Customer Support and obtain a Return Merchandise
Authorization (RMA) number. Please contact Ocean Optics for specific instructions when returning a
product.

iv 294-00000-000-02-201012

Chapter 1

Introduction

Product Overview

The HR2000+ High-Speed Miniature Fiber Optic Spectrometer provides optical resolution as good as

0.035 nm (FWHM). The HR2000+ is responsive from 200-1100 nm, but the specific range and resolution
depends on your grating and entrance slit selections. With its capability of transferring 1ms spectra
continuously, the HR2000+ is the fastest spectrometer available from Ocean Optics.

The HR2000+ is perfect for applications where fast reactions need to be monitored and high resolution is
necessary, such as chemistry and biochemistry applications.

Data programmed into a memory chip on each HR2000+ includes wavelength calibration coefficients,
linearity coefficients, and the serial number unique to each spectrometer. Our spectrometer operating
software simply reads these values from the spectrometer — a feature that enables hot swapping of
spectrometers among PCs.

The HR2000+ Spectrometer connects to a notebook or desktop PC via USB port or serial port. When
connected to the USB port of a PC, the HR2000+ draws power from the host PC, eliminating the need for
an external power supply.

Ocean Optics HR2000+ High-speed Fiber Optic Spectrometer

294-00000-000-02-201012 1

1: Introduction

System Requirements

You can use the HR2000+’s USB connectivity with any PC that meets the following requirements:

 Windows 98/Me/2000/XP operating system (or Windows CE 2.11 or later for handheld PCs)

 Ocean Optics SpectraSuite or OOIBase32 software application installed and configured for use

with the HR2000+. See

instructions.

Alternately, the HR2000+ has serial port adaptability for connecting to PCs, PLCs, and other devices that
support the RS-232 communication protocol. However, this connection method requires an external
power supply to power the HR2000+, the Breakout Box (HR4-BREAKOUT), and a serial cable.

EEPROM Utilization

An EEPROM memory chip in each HR2000+ contains wavelength calibration coefficients, linearity
coefficients, and a serial number unique to each individual spectrometer. The software application reads
these values directly from the spectrometer, enabling the ability to “hot-swap” spectrometers between PCs
without entering the spectrometer coefficients manually on each PC.

Chapter 2: Installing the HR2000+ for specific configuration

About SpectraSuite

SpectraSuite is the latest generation of operating software for all Ocean Optics spectrometers. It is a
completely modular, Java-based spectroscopy software platform that operates on Windows, Macintosh
and Linux operating systems. The software can control any Ocean Optics USB spectrometer and device,
as well as any other manufacturer’s USB instrumentation using the appropriate drivers.

SpectraSuite is a user-customizable, advanced acquisition and display program that provides a real-time
interface to a variety of signal-processing functions. With SpectraSuite, you have the ability to perform
spectroscopic measurements (such as absorbance, reflectance, and emission), control all system
parameters, collect and display data in real time, and perform reference monitoring and time acquisition
experiments. Consult the SpectraSuite manual for hardware requirements when using SpectraSuite (see

Product-Related Documentation).

Sampling System Overview

How Sampling Works

Ocean Optics components function in a sampling system as follows:

1. The user stores reference and dark measurements to correct for instrument response variables.

2. The light transmits through an optical fiber to the sample.

3. The light interacts with the sample.

4. Another optical fiber collects and transmits the result of the interaction to the spectrometer.

2 294-00000-000-02-201012

1: Introduction

5. The spectrometer measures the amount of light and transforms the data collected by the
spectrometer into digital information.

6. The spectrometer passes the sample information to SpectraSuite.

7. SpectraSuite compares the sample to the reference measurement and displays processed spectral
information.

Modular Sampling Accessories

Ocean Optics offers a complete line of spectroscopic accessories for use with the HR2000+. Most of our
spectroscopic accessories have SMA connectors for application flexibility. Accordingly, changing the
sampling system components is as easy as unscrewing a connector and replacing an accessory.

Interface Options

The HR2000+ has both USB and serial port connectors (with the use of an adapter), enabling you to
connect the spectrometer to a desktop or notebook PC via a USB port.

Computer
Interface

Desktop or
Notebook PC
via USB Port

Desktop or
Notebook PC
via Serial Port

Operating System
Requirements

Windows 98/Me/
2000/XP

Any 32-bit
Windows
operating system

Part Needed Description of Part

USB-CBL-1
(included)

HR4­BREAKOUT
(not included)

Cable that connects from USB port on HR2000+ to
USB port on desktop or notebook PC

Adapter block that enables connection from serial port
on HR2000+ to serial port on desktop or notebook PC;
comes with 5 VDC power supply (required when
connecting to serial port). User must supply own
software.

Breakout Box

Ocean Optics also offers the Breakout Box (HR4-BREAKOUT), a passive module that separates the
signals from their 30-pin port to an array of standard connectors and headers, enabling easy access to a
variety of features found in Ocean Optics’ HR2000+ Spectrometer. In addition to the accessory
connector, the breakout box features a circuit board based on a neutral breadboard pattern that allows
custom circuitry to be prototyped on the board itself.

294-00000-000-02-201012 3

1: Introduction

Shipment Components

The following information and documentation ships with the HR2000+ Spectrometer:

 Packing List

The packing list is inside a plastic bag attached to the outside of the shipment box (the invoice
arrives separately). It lists all items in the order, including customized components in the
spectrometer (such as the grating, detector collection lens, and slit). The packing list also includes
the shipping and billing addresses, as well as any items on back order.

 Spectrometer Installation Instructions

A sheet of paper that contains the information that you need to get your spectrometer system up
and running. Further information can be found on the Ocean Optics

Library

 Wavelength Calibration Data Sheet

CD (see below).

Each spectrometer is shipped with a Wavelength Calibration Data Sheet that contains information
unique to your spectrometer. SpectraSuite reads this calibration data from your spectrometer
when it interfaces to a PC via the USB port. Any other interface requires that you manually enter
the calibration data in OOIBase32 (select

Spectrometer | Configure | Wavelength Calibration

tab). See the OOIBase32 documentation for more information (refer to Product-Related

Documentation for instructions on accessing OOIBase32 documentation).

Software and Resources

Note

Please save the Wavelength Calibration Data Sheet for future reference.

 Software and Technical Resources CD

Each order ships with the Ocean Optics Software and Technical Resources CD. This disc
contains software, operating instructions, and product information for all Ocean Optics software,
spectrometers, and spectroscopic accessories. You must have Adobe Acrobat Reader version 6.0
or higher to view these files. Ocean Optics includes the Adobe Acrobat Reader on the

and Technical Resources CD

.

Ocean Optics software requires a password during the installation process. You can locate
passwords for the other software applications on the back of the

Software and Resources Library

CD package.

Software

4 294-00000-000-02-201012

Chapter 2

Installing the HR2000+

Overview

You must install the software application prior to connecting the HR2000+ Spectrometer to the PC. The
software installation installs the drivers required for HR2000+ installation. If you do not install the
software first, the system will not properly recognize the HR2000+.

If you have already connected the HR2000+ to the PC prior to installing the software, consult

Troubleshooting

for information on correcting a corrupt HR2000+ installation.

HR2000+ Installation

To connect the HR2000+ to a PC via the USB port, the PC must be running the Windows
98/ME/2000/XP operating system.

Note

The USB port on a PC can power up to five HR2000+ spectrometer channels. Systems
with more than five channels require a powered USB hub.

► Procedure

Follow the steps below to connect the HR2000+ to a PC via the USB port:

1. Install SpectraSuite on the destination computer.

2. Locate the USB cable (USB-CBL-1) provided with the HR2000+.

3. Insert the square end of the cable into the side of the HR2000+.

Chapter 3:

4. Insert the rectangular end of the cable into the USB port of the PC.

If you installed SpectraSuite prior to connecting the HR2000+, SpectraSuite installs the HR2000+ drivers.
If the drivers do not successfully install (or if you connected the HR2000+ to the PC before installing
SpectraSuite), consult

If you have followed the previous steps and started SpectraSuite, the spectrometer is already acquiring
data. Even with no light in the spectrometer, there should be a dynamic trace displayed in the bottom of
the graph. If you allow light into the spectrometer, the graph trace should rise with increasing light
intensity. This means the software and hardware are correctly installed.

Note the spectrometer(s) that you have installed are listed in the Data Sources pane.

294-00000-000-02-201012 5

Chapter 3: Troubleshooting.

2: Installing the HR2000+

Once you install the SpectraSuite software and the hardware, and establish your sampling system, you are
ready to take measurements.

Configuring the HR2000+ with OOIBase32

Once you install the HR2000+ with OOIBase32 software, you must configure OOIBase32’s Configure
Spectrometer

OOIBase32 Spectrometer Operating Software Operating Instructions for detailed instructions on
configuring the spectrometer in OOIBase32 (see

The following sections contain instructions on initially configuring the HR2000+ the first time you start
OOIBase32. Additional features are available for this spectrometer. See the

Operating Software Operating Instructions

Operator and Serial Number Dialog Box

The Operator and Serial Number screen prompts you to enter a user name and software serial number
into OOIBase32. Some data files created by OOIBase32 during sampling procedures use this information
in the file headers.

options so that OOIBase32 recognizes the HR2000+ Spectrometer. Consult the

Product-Related Documentation).

OOIBase32 Spectrometer

for detailed information on these HR2000+ features.

Default Spectrometer Configuration File

The Default Spectrometer Configuration File screen prompts you to select a spectrometer configuration
(.SPEC) file for use with the HR2000+. The unique serial number of the HR2000+ precedes the file
extension (for example, HR2A0162.SPEC).

Navigate to the OOIBase32 installation directory and select the spectrometer configuration file.

Configure Hardware Screen

The Configure Hardware screen prompts you to enter spectrometer-specific information into
OOIBase32 the first time you run the program. Typically, you need only enter this information the first
time you run OOIBase32. However, you can alter the hardware configuration at any time using the
Spectrometer Configuration screen. Select Spectrometer | Configure from the OOIBase32 menu bar
to access the

Spectrometer Configuration screen.

6 294-00000-000-02-201012

► Procedure

2: Installing the HR2000+

To configure hardware in USB Mode:

1. Specify S2000 in the

2. Specify HR2000+ in the

3. Specify the serial number of the HR2000+ under the

Spectrometer Type drop-down menu.

A/D Converter Type drop-down menu.

USB Serial Number drop-down menu.

Note

The system pre-fills this drop-down menu with the serial numbers of all discovered
HR2000+ Spectrometers.

4. Click the OK button to accept the selected options.

5. The spectrometer should now be able to acquire data and respond to light. Exit and restart
OOIBase32 to save configuration data to disk.

Spectrometer Configuration Screen

The Spectrometer Configuration screen prompts you to configure specific channel-level spectrometer
information, if necessary.

► Procedure

1. Select Spectrometer | Configure from the menu and set system parameters.

2. Select the

Wavelength Calibration tab. OOIBase32 pre-fills the coefficients for the HR2000+

from information on a memory chip in the spectrometer.

294-00000-000-02-201012 7

2: Installing the HR2000+

3. Verify that the calibration coefficients match the coefficients from the Wavelength Calibration
Data Sheet that accompanied the spectrometer. If necessary, modify these values using the USB
Programmer utility.

4. Additionally, ensure that you select both the

5. In the

A/D Interface tab, enter the same values as in the Configure Hardware screen.

OOIBase32 stores this information for future use once you close the program.

Master and Channel Enabled boxes.

Connect Spectroscopic Accessories

To find operating instructions for HR2000+-compatible products (such as light sources, sampling
chambers, and probes), consult the

http://www.oceanoptics.com/technical/operatinginstructions.asp.

Software and Technical Resources CD or the Ocean Optics website at

External Triggering Options

You can trigger the HR2000+ using a variety of External Triggering options through the 30-pin
Accessory Connector on the spectrometer. For HR2000+ firmware versions below 3.0, see the External
Triggering Options document located at

triggering.pdf

document located at ???. The triggering document contains instructions for configuring External
Triggering options for the HR2000+.

. For HR2000+ firmware version 3.0 and above, see the External Triggering Options

http://www.oceanoptics.com/technical/external-

8 294-00000-000-02-201012

Chapter 3

Troubleshooting

Overview

The following sections contain information on troubleshooting issues you may encounter when using the
HR2000+ Spectrometer.

HR2000+ Connected to PC Prior to Software
Installation

Windows Operating Systems

If you connected your Ocean Optics USB device to the computer prior to installing your Ocean Optics
software application, you may encounter installation issues that you must correct before your Ocean
Optics device will operate properly.

Follow the applicable steps below to remove the incorrectly installed device, device driver, and
installation files.

Note

If these procedures do not correct your device driver problem, you must obtain the
Correcting Device Driver Issues document from the Ocean Optics website:

http://www.oceanoptics.com/technical/engineering/correctingdevicedriverissues.pdf.

294-00000-000-02-201012 9

3: Troubleshooting

Remove the Unknown Device from Windows Device Manager

► Procedure

1. Open Windows Device Manager. Consult the Windows operating instructions for your computer
for directions, if needed.

2. Locate the
sign to the immediate left.

Other Devices option and expand the Other Devices selection by clicking on the "+"

Note

Improperly installed USB devices can also appear under the Universal Serial Bus
Controller option. Be sure to check this location if you cannot locate the unknown device.

3. Locate the unknown device (marked with a large question mark). Right-click on the Unknown

Device

4. Click the
Device. Click the

5. Disconnect the HR2000+ from your computer.

6. Locate the section in this chapter that is appropriate to your operating system and perform the
steps in the following

listing and select the Uninstall or Remove option.

OK button to continue. A warning box appears confirming the removal of the Unknown

OK button to confirm the device removal.

Remove Improperly Installed Files section.

Remove Improperly Installed Files

► Procedure

1. Open Windows Explorer.

2. Navigate to the

Windows | INF directory.

Note

If the INF directory is not visible, you must disable the Hide System Files and Folders
and Hide File Extensions for Known File Types options in Windows Folder Options.
Access Windows Folder Options from Windows Explorer, under the

Options

3. Delete the OOI_USB.INF in the INF directory. If your computer is running either the Windows
2000 or XP operating system, you must also delete the

4. Navigate to the

5. Delete the

6. Reinstall your Ocean Optics application and reboot the system when prompted.

10 294-00000-000-02-201012

menu selection.

OOI_USB.PNF file in the INF directory.

Windows | System32 | Drivers directory.

EZUSB.SYS file.

Tools | Folder

3: Troubleshooting

7. Plug in the USB device.

The system is now able to locate and install the correct drivers for the USB device.

Mac Operating Systems

Since there are no device files for the USB2000-FLG Spectrometer in a Mac operating system, you
should not encounter any problems if you installed the spectrometer before the SpectraSuite software.

Linux Operating Systems

For Linux operating systems, all you need to do is install the SpectraSuite software, then unplug and
replug in the spectrometer. Technically, the driver files for Linux simply give nonprivileged users
permission to use newly connected hardware. There isn’t any long-term harm to plugging in the device
before installing the software.

Older Version of OOIBase32 Installed

If the PC to be used to interface to your HR2000+ already has an older version of OOIBase32 software
installed, you must install the latest version of OOIBase32. You can download the latest version of
OOIBase32 from the Ocean Optics website at

http://www.oceanoptics.com/technical/softwaredownloads.asp.

You do not need to uninstall previous versions of OOIBase32 when upgrading to the latest version.

294-00000-000-02-201012 11

3: Troubleshooting

12 294-00000-000-02-201012

Appendix A

Calibrating the Wavelength of

the HR2000+

Overview

This appendix describes how to calibrate the wavelength of your spectrometer. Though each spectrometer
is calibrated before it leaves Ocean Optics, the wavelength for all spectrometers will drift slightly as a
function of time and environmental conditions. Ocean Optics recommends periodically recalibrating the
HR2000+.

About Wavelength Calibration

You are going to be solving the following equation, which shows that the relationship between pixel
number and wavelength is a third-order polynomial:



= I + C1 p + C2 p2 + C3 p

p

Where:



= the wavelength of pixel p

= the wavelength of pixel 0

I

C1 = the first coefficient (nm/pixel)

C2 = the second coefficient (nm/pixel2)

C

= the third coefficient (nm/pixel3)

3

R = the reference intensity at wavelength 

You will be calculating the value for I and the three Cs.

3

294-00000-000-02-201012 13

A: Calibrating the Wavelength of the HR2000+

Calibrating the Spectrometer

Preparing for Calibration

To recalibrate the wavelength of your spectrometer, you need the following components:

 A light source capable of producing spectral lines

Note

Ocean Optics’ HG-1 Mercury-Argon lamp is ideal for recalibration. If you do not have an
HG-1, you need a light source that produces several (at least 4-6) spectral lines in the
wavelength region of your spectrometer.

 An HR2000+ spectrometer

 An optical fiber (for spectrometers without a built-in slit, a 50-m fiber works best)

 A spreadsheet program (Excel or Quattro Pro, for example) or a calculator that performs third-

order linear regressions

Note

If you are using Microsoft Excel, choose Tools | Add-Ins and check AnalysisToolPak
and AnalysisTookPak-VBA.

Calibrating the Wavelength of the Spectrometer

► Procedure

Perform the steps below to calibrate the wavelength of the spectrometer:

1. Place the software into Scope mode and take a spectrum of your light source. Adjust the
integration time (or the A/D conversion frequency) until there are several peaks on the screen that
are not off-scale.

2. Move the cursor to one of the peaks and position the cursor so that it is at the point of maximum
intensity.

3. Record the pixel number that is displayed in the status bar or legend (located beneath the graph).
Repeat this step for all of the peaks in your spectrum.

4. Use the spreadsheet program or calculator to create a table like the one shown in the following
figure. In the first column, place the exact or true wavelength of the spectral lines that you used.

In the second column of this worksheet, place the observed pixel number. In the third column,
calculate the pixel number squared, and in the fourth column, calculate the pixel number cubed.

14 294-00000-000-02-201012

A: Calibrating the Wavelength of the HR2000+

Independent

Variable

True Wavelength (nm) Pixel # Pixel #

253.65

296.73

302.15

313.16

334.15

365.02

404.66

407.78

435.84

546.07

576.96

579.07

696.54

706.72

727.29

738.40

751.47

175
296
312
342
402
490
604
613

694
1022
1116
1122
1491
1523
1590
1627
1669

Dependent

Variables

30625
87616

97344
116964
161604
240100
364816
375769
481636

1044484
1245456
1258884
2223081
2319529
2528100
2647129
2785561

2

1067462648
1389928896
1412467848
3314613771
3532642667
4019679000
4306878883
4649101309

Pixel #

5359375
25934336
30371328
40001688
64964808

117649000
220348864
230346397
334255384

3

Values Computed

from the Regression

Output

Predicted

Wavelength

253.56

296.72

302.40

313.02

334.19

365.05

404.67

407.78

435.65

546.13

577.05

579.01

696.70

706.62

727.24

738.53

751.27

Difference

0.09

0.01

-0.25

0.13

-0.05

-0.04

-0.01

0.00

0.19

-0.06

-0.09

0.06

-0.15

0.10

0.06

-0.13

0.19

5. Use the spreadsheet or calculator to calculate the wavelength calibration coefficients. In the
spreadsheet program, find the functions to perform linear regressions.

 If using Quattro Pro, look under Tools | Advanced Math

 If using Excel, look under Analysis ToolPak

6. Select the true wavelength as the dependent variable (Y). Select the pixel number, pixel number
squared, and the pixel number cubed as the independent variables (X). After executing the
regression, you will obtain an output similar to the one shown below. Numbers of importance are
noted.

Regression Statistics

Multiple R 0.999999831
R Square 0.999999663 R Squared
Adjusted R Square 0.999999607
Standard Error 0.125540214
Observations 22

Intercept

Coefficients Standard Error

Intercept 190.473993 0.369047536 First coefficient
X Variable 1 0.36263983 0.001684745
X Variable 2-1.174416E-05 8.35279E-07
X Variable 3-2.523787E-09 2.656608E-10 Second coefficient

Third coefficient

294-00000-000-02-201012 15

A: Calibrating the Wavelength of the HR2000+

7. Record the Intercept, as well as the First, Second, and Third Coefficients. Additionally, look at
the value for R squared. It should be very close to 1. If not, you have most likely assigned one of
your wavelengths incorrectly.

Keep these values at hand.

Saving the New Calibration Coefficients: USB
Mode

Ocean Optics programs wavelength calibration coefficients unique to each HR2000+ onto an EEPROM
memory chip in the HR2000+.

You can overwrite old calibration coefficients on the EEPROM if you are using the HR2000+ via the
USB port. If you are using the HR2000+ via the serial port, consult the

Coefficients: Serial Mode section later in this appendix.

► Procedure

To save wavelength calibration coefficients using the USB mode, perform the following steps:

1. Ensure that the HR2000+ is connected to the PC and that you have closed all other applications.

Saving the New Calibration

2. Point your browser to
scroll down to

3. Save the setup file to your computer.

4. Run the

5. Click the

6. Accept the default installation location, or click the
click the

7. Click the

8. Click the

Complete

9. Click the

10. Navigate to the

11. Click on the desired HR2000+ device displayed in the left pane of the

12. Double-click on each of the calibration coefficients displayed in the right pane of the USB
Programmer screen and enter the new values acquired in Steps 5 and 6 of the

Wavelength of the Spectrometer section in this appendix.

13. Repeat Step 12 for all of the new values.

14. Click on the
software.

Microcode. Select USB EEPROM Programmer.

Setup.exe file to install the software. The Welcome screen appears.

Next button. The Destination Location screen appears.

Next button. The Program Manager Group screen appears.

Next button. The Start Installation screen appears.

Next button to begin the installation. Once the installation finishes, the Installation

screen appears.

Finish button and reboot the computer when prompted.

Save All Values button to save the information, and then Exit the USB Programmer

http://www.oceanoptics.com/technical/softwaredownloads.asp and

Browse button to specify a directory. Then,

USB EEPROM Programmer from the Start menu and run the software.

USB Programmer screen.

Calibrating the

The new wavelength calibration coefficients are now loaded onto the EEPROM memory chip on the
HR2000+.

16 294-00000-000-02-201012

A: Calibrating the Wavelength of the HR2000+

Saving the New Calibration Coefficients: Serial
Mode

If you are connecting the HR2000+ Spectrometer to the serial port of the PC, you need to save the new
wavelength calibration coefficients to the

You cannot save the calibration coefficients to the EEPROM memory chip on the
HR2000+ when using the serial mode.

► Procedure

To save Wavelength Calibration Coefficients using the Serial mode, perform the following steps:

1. Open the OOIBase32 application.

.SPEC file that OOIBase32 accesses when opened.

Note

2. Select

3. Select the

4. Enter in the new values acquired from Steps 5 and 6 of the

5. Click the

Spectrometer | Configure from the OOIBase32 menu bar. The Configure Spectrometer

screen appears.

Wavelength Calibration tab to update the wavelength coefficients within OOIBase32.

Spectrometer section in this appendix.

OK button to save the information in OOIBase32.

Calibrating the Wavelength of the

294-00000-000-02-201012 17

A: Calibrating the Wavelength of the HR2000+

18 294-00000-000-02-201012

Appendix B

Specifications

Overview

This appendix contains information on spectrometer operation, specifications, and system compatibility.
It also includes accessory connector pinout diagrams and pin-specific information.

How the HR2000+ Works

Below is a diagram of how light moves through the optical bench of an HR2000+ Spectrometer. The
optical bench has no moving parts that can wear or break; all the components are fixed in place at the time
of manufacture. Items with an asterisk (*) are user-specified.

HR2000+ Spectrometer with Components

See HR2000+ Components Table on the following page for an explanation of the function of each
numbered component in the HR2000+ Spectrometer in this diagram.

294-00000-000-02-201012 19

B: HR2000+ Specifications

HR2000+ Components Table

Ocean Optics permanently secures all components in the HR2000+ at the time of manufacture. Only
Ocean Optics technicians can replace interchangeable components, where noted.

Item Name Description

1

2 Slit

3 Filter

4

5 Grating

SMA
Connector

Collimating
Mirror

Secures the input fiber to the spectrometer. Light from the input fiber enters the
optical bench through this connector.

A dark piece of material containing a rectangular aperture, which is mounted directly
behind the SMA Connector. The size of the aperture regulates the amount of light
that enters the optical bench and controls spectral resolution.

You can also use the HR2000+ without a Slit. In this configuration, the diameter of
the fiber connected to the HR2000+ determines the size of the entrance aperture.

Only Ocean Optics technicians can change the Slit.

Restricts optical radiation to pre-determined wavelength regions. Light passes
through the Filter before entering the optical bench. Both bandpass and longpass
filters are available to restrict radiation to certain wavelength regions.

Only Ocean Optics technicians can change the Filter.

Focuses light entering the optical bench towards the Grating of the spectrometer.

Light enters the spectrometer, passes through the SMA Connector, Slit, and Filter,
and then reflects off the Collimating Mirror onto the Grating.

Diffracts light from the Collimating Mirror and directs the diffracted light onto the
Focusing Mirror. Gratings are available in different groove densities, allowing you to
specify wavelength coverage and resolution in the spectrometer.

Only Ocean Optics technicians can change the Grating.

6

7

8

20 294-00000-000-02-201012

Focusing
Mirror

L2 Detector
Collection
Lens

CCD
Detector
(UV or VIS)

Receives light reflected from the Grating and focuses the light onto the CCD
Detector or L2 Detector Collection Lens (depending on the spectrometer
configuration).

An optional component that attaches to the CCD Detector. It focuses light from a tall
slit onto the shorter CCD Detector elements.

The L2 Detector Collection Lens should be used with large diameter slits or in
applications with low light levels. It also improves efficiency by reducing the effects
of stray light.

Only Ocean Optics technicians can add or remove the L2 Detection Collection Lens.

Collects the light received from the Focusing Mirror or L2 Detector Collection Lens
and converts the optical signal to a digital signal.

Each pixel on the CCD Detector responds to the wavelength of light that strikes it,
creating a digital response. The spectrometer then transmits the digital signal to the
OOIBase32 application.

B: HR2000+ Specifications

HR2000+ Specifications

The following sections provide specification information for the CCD detector in the HR2000+, as well
as the HR2000+ Spectrometer itself. HR2000+CG-UV-NIR specifications are listed in

Error! Reference source not found..

CCD Detector Specifications

Specification Value

Detector Sony ILX-511B linear silicon CCD array

No. of elements 2048 pixels

Appendix C:

Sensitivity

Pixel size 14 µm x 200 µm

Pixel well depth 65 Ke-

Signal-to-noise ratio 250:1 (at full signal)

A/D resolution 14 bit

Dark noise 3.2 RMS counts

Corrected linearity >99.8%

Maximum pixel rate Rate at which pixels are digitized is 1 MHz

75 photons per count at 400 nm
41 photons per count at 600 nm

HR2000+ Spectrometer

Specification Value

Dimensions 148.6 mm x 104.8 mm x 45.1 mm

Weight 570 g

Power consumption 220 mA @ 5 VDC

Detector 2048-element linear silicon CCD array

Detector range 200-1100 nm

Gratings 14 gratings available

294-00000-000-02-201012 21

B: HR2000+ Specifications

Specification Value

Entrance aperture 5, 10, 25, 50, 100 or 200 µm wide slits

Order-sorting filters Installed longpass and bandpass filters

Focal length f/4, 101 mm

Optical resolution Depends on grating and size of entrance aperture

Stray light <0.05% at 600 nm; <0.10% at 435 nm

Dynamic range 2 x 108 (system); 1300:1 for a single acquisition

Fiber optic connector SMA 905 to single-strand optical fiber (0.22 NA)

Data transfer rate:

USB 2.0 Port

Serial Port

Integration time 1 ms to 65 seconds

Interfaces

Operating systems:

USB Port

Serial Port

Onboard GPIO 10 user-programmable digital I/Os

Analog channels One 13-bit analog input and one 9-bit analog output

Full scans into memory every 1 millisecond

Full scans into memory every 600 milliseconds

USB 2.0, 480 Mbps (USB 1.1 compatible); RS-232 (2-wire); SPI
(3-wire); I

Windows 2000/XP, Mac OS X, and Linux

Any 32-bit Windows operating system

2

C Inter-Integrated Circuit 2-wire serial bus

System Compatibility

The following sections provide information on hardware and software requirements for the HR2000+.

Compatibility for Desktop or Notebook PCs

To use the HR2000+, you must have a PC that meets the following minimum requirements:

 Operating systems: Windows 2000/XP, Mac OS X, or Linux with USB port; any 32-bit or 64-bit

Windows OS with serial port

 Computer interfaces: USB 2.0 @ 480 Mbps; RS-232 (2-wire) @ 115.2 K baud

 Peripheral interfaces: I2C inter-integrated circuit

22 294-00000-000-02-201012

B: HR2000+ Specifications

30-Pin Accessory Connector Pinout

The HR2000+ features a 30-pin Accessory Connector, located on the side of the unit as shown:

Location of HR2000+ 30-Pin Accessory Connector

30-Pin Accessory Connector Pinout Diagram

When facing the 30-pin Accessory Connector on the front of the vertical wall of the HR2000+, pin
numbering is as follows:

USB

Port

2 4 6 8 10 12 14 16 18 20 22 24 26 28 30

1 3 5 7 9 11 13 15 17 19 21 23 25 27 29

30-Pin Accessory Connector Pinout Diagram

30-Pin Accessory Connector – Pin Definitions and
Descriptions

The following table contains information regarding the function of each pin in the HR2000+’s 30-Pin
Accessory Connector:

Pin

Function Input/Output Description

#

1 RS232 Rx Input RS232 receive signal – Communicates with a PC over DB9 Pin 3

2 RS232 Tx Output RS232 transmit signal – Communicates with a PC over DB9 Pin 2

3 GPIO (2) Input/Output

General purpose software-programmable, digital input/output
(channel number) Reserved

4 V5_SW Output Regulated 5 Volt power pin – Supplies 50 mA (maximum)

294-00000-000-02-201012 23

B: HR2000+ Specifications

Pin

Function Input/Output Description

#

5 Ground Input/Output Ground

6 I2C SCL Input/Output I2C clock signal for communication to other I2C peripherals

7 GPIO (0) Input/Output

General purpose software-programmable, digital input/output
(channel number) Reserved

8 I2C SDA Input/Output I2C data signal for communication to other I2C peripherals

9 GPIO (1) Input/Output

10

Ext.
Trigger In

Input

11 GPIO (3) Input/Output

V

, V

12

CC

or 5V

IN

USB

,

Input or Output

General purpose software-programmable, digital input/output
(channel number) Reserved

TTL input trigger signal – See External Triggering Options
document for information.

General purpose software-programmable, digital input/output
(channel number) Reserved

Input power pin for HR2000+ – When operating via USB, this pin
can power other peripherals – Ensure that peripherals comply with
USB specifications

13 Reserved Output Reserved

Input power pin for HR2000+ – When operating via USB, this pin
can power other peripherals – Ensure that peripherals comply with
USB specifications

14

V

, V

CC

or 5V

IN

USB

,

Input or Output

15 Reserved Input Reserved

16 GPIO (4) Input /Output

17

Single
Strobe

Output

18 GPIO (5) Input/Output

General purpose software-programmable, digital input/output
(channel number) Reserved

TTL output pulse used as a strobe signal – Has a programmable
delay relative to the beginning of the spectrometer integration
period

General purpose software-programmable, digital input/output
(channel number) Reserved

19 Reserved Output Reserved

Continuous

20

Strobe

SPI Chip

21

Select

24 294-00000-000-02-201012

Output

Output

TTL output signal used to pulse a strobe – Divided down from the
master clock signal

SPI Chip/Device Select signal for communication to other SPI
peripherals

Pin

Function Input/Output Description

#

B: HR2000+ Specifications

22 GPIO (6) Input/Output

Analog In

23

(0-5V)

Analog Out

24

(0-5V)

Lamp

25

Enable

26 GPIO (7) Input/Output

27 Ground Input/Output Ground

28 GPIO (8) Input/Output

29 Ground Input/Output Ground

30 GPIO (9) Input/Output

Input 13-bit analog-to-digital input with a 0-5V range. For future use.

Output 9-bit programmable output voltage with a 0-5V range

Output

General purpose software-programmable, digital input/output
(channel number) Reserved

TTL signal driven Active HIGH when the Lamp Enable command
is sent to the spectrometer

General purpose software-programmable, digital input/output
(channel number) Reserved

General purpose software-programmable, digital input/output
(channel number) Reserved

General purpose software-programmable, digital input/output
(channel number) Reserved

30-Pin J2 Accessory Connector - Part Numbers

The part numbers for the 30-pin accessory connector on the HR2000+ Spectrometer are as follows:

 The connector is Pak50™ model from 3M Corp. Headed Connector – Part Number

P50–030P1–RR1–TG.

 The mating connector is Part Number P50–030S–TGF.

 Mating the two components requires two 1.27 mm (50 mil) flat ribbon cables (3M 3365 Series is

recommended).

If you are customizing your HR2000+ Spectrometer system or configuring External Triggering, you may
need these part numbers to complete your setup.

294-00000-000-02-201012 25

B: HR2000+ Specifications

HR2000+ 15-Pin Accessory Cable Pinout

Pin # Description Pin # Description

1 Single_strobe 9 GPIO-9

2 ContStrobe 10 GND_SIGNAL

3 V5_SW 11 SDA

4 ExtTrigIn 12 SCL

5 ExtTrigIn 13 LampEnable

6 GPIO-8 14 A_IN

7 A_OUT 15 GPIO-7

8 ExtTrigIn

26 294-00000-000-02-201012

Index

Numbers

15-pin accessory cable

pinouts, 26

30-pin accessory connector

diagram, 23
part numbers, 25
pin definitions, 23

A

accessories, 8
Accessories, 3
accessory connector

pinout, 23

Adobe Acrobat Reader, 4

B

breakout box, 3

C

Calibrating, iii, 13
calibration, 13

preparing for, 14
procedure, 14

calibration coefficients

saving in Serial mode, 17

saving in USB mode, 16
CCD, 21
CCD Detector, 20
collimating mirror, 20
compatibility, 22

Desktop or Notebook PCs, 22
Components Table, 20
Configure Hardware, 6
configuring, 6

D

Default Spectrometer Configuration File, 6
detector, 21
Detector Collection Lens, 20
document

audience, iii
purpose, iii
summary, iii

E

EEPROM, 2
External Triggering, 8

F

filter, 20
focusing mirror, 20

G

grating, 20

I

Installation, 5

USB mode, 5
installed filter, 20
Interface, 3

L

L2 Detector Collection Lens, 20
Lens, 20

294-00000-000-02-201012 27

Index

M

memory chip, 2
mirror, 20

O

OOIBase32

configuring, 6

Options

Interface, 3

P

packing list, 4
passwords, 4
power supply (external), 2
product-related documentation, iii

S

Sampling

Accessories, 3

System, 2
serial number, 6
setup, 5
shipment components, 4
slit, 20
SMA Connector, 20
Software and Resources Library CD, 4
specifications, 19, 21

detector, 21

spectrometer, 21
SpectraSuite, 2
Spectrometer Configuration Screen, 7
spectrometer operation, 19
spectroscopic accessories, 8
System Requirements, 2

T

Triggering, 8
troubleshooting

Linux systems, 11
Mac systems, 11
OOIBase32, 11
Windows systems, 9

Troubleshooting, 9

U

upgrades, iv
USB mode, 5
USB-ADP-PC, 3
USB-CBL-1, 3

W

Wavelength Calibration

about, 13
Wavelength Calibration Data File, 4
Wavelength Calibration Data Sheet, 4
what's new, iii

28 294-00000-000-02-201012