Ocean Optics HR2000+ User Manual

HR2000+ High-speed Fiber Optic Spectrometer

Installation and Operation Manual

Document Number 294-00000-000-02-201012

Offices: Ocean Optics, Inc. World Headquarters

830 Douglas Ave., Dunedin, FL, USA 34698

Phone [phone]
Fax [phone]

8 a.m.– 8 p.m. (Mon-Thu), 8 a.m.– 6 p.m. (Fri) EST

E-mail: [e-mail] (General sales inquiries)

[e-mail] (Questions about orders)
[e-mail] (Technical support)

000-00000-000-02-A

Additional
Offices:

Regional Headquarters

Maybachstrasse 11
73760 Ostfildern

Phone [phone]
Fax [phone]
E-Mail [e-mail]

Ocean Optics Asia

666 Gubei Road, Kirin Tower, Suite 601B, Changning District, Shanghai,
PRC. 200336

Phone [phone]

Fax [phone]
E-Mail [e-mail]

Ocean Optics EMEA

Sales and Support Center
Geograaf 24, 6921 EW DUIVEN, The Netherlands

Phone [phone]

Fax [phone]
E-Mail

[e-mail]

Copyright © 2010 Ocean Optics, Inc.

All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, by any means, electronic, mechanical,
photocopying, recording, or otherwise, without written permission from Ocean Optics, Inc.
This manual is sold as part of an order and subject to the condition that it shall not, by way of trade or otherwise, be lent, re-sold, hired out or otherwise
circulated without the prior consent of Ocean Optics, Inc. in any form of binding or cover other than that in which it is published.

Trademarks

All products and services herein are the trademarks, service marks, registered trademarks or registered service marks of their respective owners.

Limit of Liability

Every effort has been made to make this manual as complete and as accurate as possible, but no warranty or fitness is implied. The information provided is
on an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity with respect to any loss or damages arising from
the information contained in this manual.

Table of Contents

About This Manual......................................................................................................... iii

Document Purpose and Intended Audience..............................................................................iii

What’s New in this Document ................................................................................................... iii

Document Summary..................................................................................................................iii

Product-Related Documentation.................................................................................... iii

Upgrades....................................................................................................................... iv

Chapter 1: Introduction .....................................................................1

Product Overview .......................................................................................................... 1

System Requirements ................................................................................................... 2

EEPROM Utilization .................................................................................................................. 2

About SpectraSuite.................................................................................................................... 2

Sampling System Overview....................................................................................................... 2

How Sampling Works............................................................................................................2

Modular Sampling Accessories............................................................................................. 3

Interface Options ........................................................................................................... 3

Breakout Box ............................................................................................................................. 3

Shipment Components .................................................................................................. 4

Chapter 2: Installing the HR2000+....................................................5

Overview ....................................................................................................................... 5

HR2000+ Installation ..................................................................................................... 5

Configuring the HR2000+ with OOIBase32 .............................................................................. 6

Operator and Serial Number Dialog Box .............................................................................. 6

Default Spectrometer Configuration File ............................................................................... 6

Configure Hardware Screen .................................................................................................6

Spectrometer Configuration Screen......................................................................................7

Connect Spectroscopic Accessories.............................................................................. 8

External Triggering Options........................................................................................... 8

294-00000-000-02-201012 i

Table of Contents

Chapter 3: Troubleshooting..............................................................9

Overview ....................................................................................................................... 9

HR2000+ Connected to PC Prior to Software Installation .............................................. 9

Windows Operating Systems .................................................................................................... 9

Remove the Unknown Device from Windows Device Manager ...........................................10

Remove Improperly Installed Files........................................................................................10

Mac Operating Systems ............................................................................................................11

Linux Operating Systems .......................................................................................................... 11

Older Version of OOIBase32 Installed........................................................................... 11

Appendix A: Calibrating the Wavelength of the HR2000+ ..............13

Overview ....................................................................................................................... 13

About Wavelength Calibration ....................................................................................... 13

Calibrating the Spectrometer ......................................................................................... 14

Preparing for Calibration............................................................................................................ 14

Calibrating the Wavelength of the Spectrometer ......................................................................14

Saving the New Calibration Coefficients: USB Mode ..................................................... 16

Saving the New Calibration Coefficients: Serial Mode ................................................... 17

Appendix B: Specifications...............................................................19

Overview ....................................................................................................................... 19

How the HR2000+ Works .............................................................................................. 19

HR2000+ Components Table .................................................................................................... 20

HR2000+ Specifications ................................................................................................ 21

CCD Detector Specifications ..................................................................................................... 21

HR2000+ Spectrometer............................................................................................................. 21

System Compatibility ..................................................................................................... 22

Compatibility for Desktop or Notebook PCs .............................................................................. 22

30-Pin Accessory Connector Pinout .............................................................................. 23

30-Pin Accessory Connector Pinout Diagram ........................................................................... 23

30-Pin Accessory Connector – Pin Definitions and Descriptions.............................................. 23

30-Pin J2 Accessory Connector - Part Numbers.......................................................................25

HR2000+ 15-Pin Accessory Cable Pinout ..................................................................... 26

Index ................................................................................................... 27

ii 294-00000-000-02-201012

About This Manual

Document Purpose and Intended Audience

This document provides the user of HR2000+ Spectrometer with instructions for setting up, calibrating
and performing experiments with their spectrometer.

What’s New in this Document

This version of the HR2000+ High-speed Fiber Optic Spectrometer, Installation and Operation Manual
updates information for the new triggering options for firmware versions 3.0 and above.

Document Summary

Chapter Description

Chapter 1: Introduction

Chapter 2: Installing the HR2000+

Chapter 3: Troubleshooting

Appendix A: Calibrating the

Wavelength of the HR2000+

Appendix B: Specifications

Contains descriptive information about the HR2000+
Spectrometer and how sampling works. It also provides a list
of system requirements, interface options, and shipment
components.

Provides installation and configuration instructions.

Contains recommended steps to isolate and correct common
problems.

Provides instructions for calibrating the HR2000+
Spectrometers.

Contains technical specifications and connector pinouts for the
HR2000+ Spectrometers.

Product-Related Documentation

You can access documentation for Ocean Optics products by visiting our website at

http://www.oceanoptics.com. Select Technical → Operating Instructions, then choose the appropriate

document from the available drop-down lists. Or, use the
of the web page.

Search by Model Number field at the bottom

 Detailed instructions for SpectraSuite Spectrometer Operating Software are located at:

http://www.oceanoptics.com/technical/SpectraSuite.pdf.

 Detailed instructions for the OOIBase32 Spectrometer Operating Software are located at:

http://www.oceanoptics.com/technical/ooibase32.pdf

294-00000-000-02-201012 iii

About This Manual

 Detailed instructions for the Breakout Box are located at:

http://www.oceanoptics.com/technical/HR4_breakout.pdf.

 Detailed instructions for External Triggering for firmware versions below 3.0 are located at:

http://www.oceanoptics.com/technical/external-triggering.pdf.

 Detailed instructions for External Triggering for firmware versions 3.0 and above are located at:

http://www.oceanoptics.com/technical/external-triggering2.pdf.

Engineering-level documentation is located on our website at

You can also access operating instructions for Ocean Optics products from the Software and Technical

Resources

CD that ships with the product.

Technical → Engineering Docs.

Upgrades

Occasionally, you may find that you need Ocean Optics to make a change or an upgrade to your system.
To facilitate these changes, you must first contact Customer Support and obtain a Return Merchandise
Authorization (RMA) number. Please contact Ocean Optics for specific instructions when returning a
product.

iv 294-00000-000-02-201012

Chapter 1

Introduction

Product Overview

The HR2000+ High-Speed Miniature Fiber Optic Spectrometer provides optical resolution as good as

0.035 nm (FWHM). The HR2000+ is responsive from 200-1100 nm, but the specific range and resolution
depends on your grating and entrance slit selections. With its capability of transferring 1ms spectra
continuously, the HR2000+ is the fastest spectrometer available from Ocean Optics.

The HR2000+ is perfect for applications where fast reactions need to be monitored and high resolution is
necessary, such as chemistry and biochemistry applications.

Data programmed into a memory chip on each HR2000+ includes wavelength calibration coefficients,
linearity coefficients, and the serial number unique to each spectrometer. Our spectrometer operating
software simply reads these values from the spectrometer — a feature that enables hot swapping of
spectrometers among PCs.

The HR2000+ Spectrometer connects to a notebook or desktop PC via USB port or serial port. When
connected to the USB port of a PC, the HR2000+ draws power from the host PC, eliminating the need for
an external power supply.

Ocean Optics HR2000+ High-speed Fiber Optic Spectrometer

294-00000-000-02-201012 1

1: Introduction

System Requirements

You can use the HR2000+’s USB connectivity with any PC that meets the following requirements:

 Windows 98/Me/2000/XP operating system (or Windows CE 2.11 or later for handheld PCs)

 Ocean Optics SpectraSuite or OOIBase32 software application installed and configured for use

with the HR2000+. See

instructions.

Alternately, the HR2000+ has serial port adaptability for connecting to PCs, PLCs, and other devices that
support the RS-232 communication protocol. However, this connection method requires an external
power supply to power the HR2000+, the Breakout Box (HR4-BREAKOUT), and a serial cable.

EEPROM Utilization

An EEPROM memory chip in each HR2000+ contains wavelength calibration coefficients, linearity
coefficients, and a serial number unique to each individual spectrometer. The software application reads
these values directly from the spectrometer, enabling the ability to “hot-swap” spectrometers between PCs
without entering the spectrometer coefficients manually on each PC.

Chapter 2: Installing the HR2000+ for specific configuration

About SpectraSuite

SpectraSuite is the latest generation of operating software for all Ocean Optics spectrometers. It is a
completely modular, Java-based spectroscopy software platform that operates on Windows, Macintosh
and Linux operating systems. The software can control any Ocean Optics USB spectrometer and device,
as well as any other manufacturer’s USB instrumentation using the appropriate drivers.

SpectraSuite is a user-customizable, advanced acquisition and display program that provides a real-time
interface to a variety of signal-processing functions. With SpectraSuite, you have the ability to perform
spectroscopic measurements (such as absorbance, reflectance, and emission), control all system
parameters, collect and display data in real time, and perform reference monitoring and time acquisition
experiments. Consult the SpectraSuite manual for hardware requirements when using SpectraSuite (see

Product-Related Documentation).

Sampling System Overview

How Sampling Works

Ocean Optics components function in a sampling system as follows:

1. The user stores reference and dark measurements to correct for instrument response variables.

2. The light transmits through an optical fiber to the sample.

3. The light interacts with the sample.

4. Another optical fiber collects and transmits the result of the interaction to the spectrometer.

2 294-00000-000-02-201012

1: Introduction

5. The spectrometer measures the amount of light and transforms the data collected by the
spectrometer into digital information.

6. The spectrometer passes the sample information to SpectraSuite.

7. SpectraSuite compares the sample to the reference measurement and displays processed spectral
information.

Modular Sampling Accessories

Ocean Optics offers a complete line of spectroscopic accessories for use with the HR2000+. Most of our
spectroscopic accessories have SMA connectors for application flexibility. Accordingly, changing the
sampling system components is as easy as unscrewing a connector and replacing an accessory.

Interface Options

The HR2000+ has both USB and serial port connectors (with the use of an adapter), enabling you to
connect the spectrometer to a desktop or notebook PC via a USB port.

Computer
Interface

Desktop or
Notebook PC
via USB Port

Desktop or
Notebook PC
via Serial Port

Operating System
Requirements

Windows 98/Me/
2000/XP

Any 32-bit
Windows
operating system

Part Needed Description of Part

USB-CBL-1
(included)

HR4­BREAKOUT
(not included)

Cable that connects from USB port on HR2000+ to
USB port on desktop or notebook PC

Adapter block that enables connection from serial port
on HR2000+ to serial port on desktop or notebook PC;
comes with 5 VDC power supply (required when
connecting to serial port). User must supply own
software.

Breakout Box

Ocean Optics also offers the Breakout Box (HR4-BREAKOUT), a passive module that separates the
signals from their 30-pin port to an array of standard connectors and headers, enabling easy access to a
variety of features found in Ocean Optics’ HR2000+ Spectrometer. In addition to the accessory
connector, the breakout box features a circuit board based on a neutral breadboard pattern that allows
custom circuitry to be prototyped on the board itself.

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1: Introduction

Shipment Components

The following information and documentation ships with the HR2000+ Spectrometer:

 Packing List

The packing list is inside a plastic bag attached to the outside of the shipment box (the invoice
arrives separately). It lists all items in the order, including customized components in the
spectrometer (such as the grating, detector collection lens, and slit). The packing list also includes
the shipping and billing addresses, as well as any items on back order.

 Spectrometer Installation Instructions

A sheet of paper that contains the information that you need to get your spectrometer system up
and running. Further information can be found on the Ocean Optics

Library

 Wavelength Calibration Data Sheet

CD (see below).

Each spectrometer is shipped with a Wavelength Calibration Data Sheet that contains information
unique to your spectrometer. SpectraSuite reads this calibration data from your spectrometer
when it interfaces to a PC via the USB port. Any other interface requires that you manually enter
the calibration data in OOIBase32 (select

Spectrometer | Configure | Wavelength Calibration

tab). See the OOIBase32 documentation for more information (refer to Product-Related

Documentation for instructions on accessing OOIBase32 documentation).

Software and Resources

Note

Please save the Wavelength Calibration Data Sheet for future reference.

 Software and Technical Resources CD

Each order ships with the Ocean Optics Software and Technical Resources CD. This disc
contains software, operating instructions, and product information for all Ocean Optics software,
spectrometers, and spectroscopic accessories. You must have Adobe Acrobat Reader version 6.0
or higher to view these files. Ocean Optics includes the Adobe Acrobat Reader on the

and Technical Resources CD

.

Ocean Optics software requires a password during the installation process. You can locate
passwords for the other software applications on the back of the

Software and Resources Library

CD package.

Software

4 294-00000-000-02-201012

Chapter 2

Installing the HR2000+

Overview

You must install the software application prior to connecting the HR2000+ Spectrometer to the PC. The
software installation installs the drivers required for HR2000+ installation. If you do not install the
software first, the system will not properly recognize the HR2000+.

If you have already connected the HR2000+ to the PC prior to installing the software, consult

Troubleshooting

for information on correcting a corrupt HR2000+ installation.

HR2000+ Installation

To connect the HR2000+ to a PC via the USB port, the PC must be running the Windows
98/ME/2000/XP operating system.

Note

The USB port on a PC can power up to five HR2000+ spectrometer channels. Systems
with more than five channels require a powered USB hub.

► Procedure

Follow the steps below to connect the HR2000+ to a PC via the USB port:

1. Install SpectraSuite on the destination computer.

2. Locate the USB cable (USB-CBL-1) provided with the HR2000+.

3. Insert the square end of the cable into the side of the HR2000+.

Chapter 3:

4. Insert the rectangular end of the cable into the USB port of the PC.

If you installed SpectraSuite prior to connecting the HR2000+, SpectraSuite installs the HR2000+ drivers.
If the drivers do not successfully install (or if you connected the HR2000+ to the PC before installing
SpectraSuite), consult

If you have followed the previous steps and started SpectraSuite, the spectrometer is already acquiring
data. Even with no light in the spectrometer, there should be a dynamic trace displayed in the bottom of
the graph. If you allow light into the spectrometer, the graph trace should rise with increasing light
intensity. This means the software and hardware are correctly installed.

Note the spectrometer(s) that you have installed are listed in the Data Sources pane.

294-00000-000-02-201012 5

Chapter 3: Troubleshooting.