Nuaire NU-140E User guide

AIREGARD™ ES Energy Saver Vertical Laminar Flow

Clean Workstation

Model NU-140E

Bench Top Model

Operation and Maintenance Manual

July, 2020

Revision 5

Series A

(230VAC ONLY)

Manufactured By:

NuAire, Inc.

2100 Fernbrook Lane

Plymouth, MN 55447

Toll-Free: 1-800-328-3352

In Minnesota: (763)-553-1270

Fax: [phone]

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AIREGARD™ ES Energy Saver Vertical Laminar Flow

Clean Workstation

Model NU-140E

Bench Top Model

Operation and Maintenance Manual

Table of Contents

Section No. 1 ........................................................................... General Description

Section No. 2 ........................................................................... Models & Features

Section No. 3 ........................................................................... Shipments

Section No. 4 ........................................................................... Installation Instructions

4.1 ................................................................................... Location

4.2 ................................................................................... Set-up Instructions

4.3 ................................................................................... Certification Testing Methods and Equipment

Section No. 5 ........................................................................... Operating the Laminar Flow Clean Workstation

5.1 ................................................................................... Operator Controls & Indicators

5.2 ................................................................................... Operating Guidelines

5.3 ................................................................................... Ergonomics

5.4 ................................................................................... Cleaning Procedures

Section No. 6 ........................................................................... General Maintenance

6.1 ................................................................................... Fluorescent Lamp Bulb Replacement

6.2 ................................................................................... HEPA Filter Replacement

6.3 ................................................................................... Prefilter Replacement

Section No. 7 ........................................................................... Error Indicators and Troubleshooting

Section No. 8 ........................................................................... Polycarbonate Material Compatibility

Section No. 9 ........................................................................... Remote Contacts

Section No. 10 ......................................................................... Optional Equipment

10.1 ................................................................................. Ultraviolet Lamp

10.2 ................................................................................. Electrical/Environmental Requirements

Section No. 11 ......................................................................... Electrical/Environmental Requirements

Section No. 12 ......................................................................... Disposal and Recycle

Insert ....................................................................................... Replacement Parts List, Warranty

Manual Drawings

BCD-19868..........................................................Specification Drawing NU-140-330

BCD-19847..........................................................Specification Drawing NU-140-430

BCD-19869..........................................................Specification Drawing NU-140-530

BCD-19665..........................................................Specification Drawing NU-140-630

Assembly Drawings

ACD-06899..........................................................Blower Brace Removal

BCD-19015..........................................................Base Stand Telescoping with Leg Levelers

BCD-19016..........................................................Base Stand Telescoping with Caster

CD-000018..........................................................Hydraulic Base Stand with Castors

CD-000019..........................................................Hydraulic Base Stand with Leg Levelers

BCD-16521..........................................................AEROMAX™ Front Panel

BCD-19998..........................................................HEPA Filter Replacement

BCD-20293..........................................................Outlet Option Installation

Electrical Schematics

BCD-19859..........................................................Electrical Schematic 230V

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1.0 General Description

NuAire AIREGARD™ Vertical Laminar Flow Clean Workstations utilize the newest technologies in laminar airflow design,
materials and manufacturing processes. The clean workstation can be used where clean airflow per ISO 14644-1 is
required for the preparation of injectable drugs, IV solutions, tissue culture, optics, microelectronics, etc. The clean bench
should not be used for any work that involves biological agents assigned a level of Risk 1 through 4 as classified by the
Centers for Disease Control (CDC), Atlanta, Georgia, since the Vertical flow offers no personnel protection against these
agents. The clean bench should only be used to protect the product from contamination. The clean bench is optionally
available with a base stand, placing the work surface at 30” (762mm) or 36” (914mm). A significant number of design
innovations give the NuAire Laminar flow equipment superior performance qualities in airflow, lighting, noise levels and
vibration.

AIREGARD™ ES Energy Saver Vertical Laminar Flow

Clean Workstation

Model NU-140E

Manufactured By:

NuAire, Inc.,

Plymouth, Minnesota

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1.1 Safety Instructions

NOTE:

CAUTION used without the safety alert symbol

not avoided, may result in property damage.

Potential electrical hazard, only qualified person
to access.

Used for important information.

Biohazard

Ground, Earth

CAUTION:



Lead Free

Chemical Hazard
Flammable Hazard

Hazardous Gases! Personal

Safety alert symbol indicates a potentially hazardous

of serious injury.

Safety alert symbol indicates a potentially hazardous

moderate injury.

WARNING

!

CAUTION

!

These safety instructions describe the safety features of the AIREGARD™ Vertical Laminar Flow Clean Workstation model
NU-140E. The workstation has been manufactured using the latest technological developments and has been thoroughly
tested before delivery. It may, however, present potential hazards if it is not used according to the intended purpose or
outside of operating parameters. Therefore, the following procedures must always be observed:

• The workstation must be operated only by trained and authorized personnel.

• For any operation of this unit, the operator must prepare clear and concise written instructions for operating and

cleaning, utilizing applicable safety data sheets, plant hygiene guidelines, and technical regulations, in particular.

o which decontamination measures are to be applied for the workstation and accessories,
o which measures are to be taken in the case of an accident.

• Repairs to the device must be carried out only by trained and authorized expert personnel.

• Keep these operating instructions close to the unit so that safety instructions and important information are

always accessible.

• Should you encounter problems that are not detailed adequately in the operating instructions, please contact your
NuAire Representative of NuAire technical Services.

1.2 Explanation of Symbols

indicates a potentially hazardous situation which, if

situation which, if not avoided, could result in death

situation which, if not avoided, may result in minor or

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Protection Equipment Required.

2.0 Models and Features

Model Number

NU-140-330E

NU-140-430E

NU-140-530E

NU-140-630E

NuAire's Model Number NU-140E designates the basic design series of AIREGARD™ Vertical Laminar Flow Clean
Workstation with the blower/motor located above the work surface (i.e. bench series). Model numbers are shown below.

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3.0 Shipments

NuAire takes every reasonable precaution to insure that your AIREGARD™ Clean Workstation arrives without damage.
Motor carriers are carefully selected and shipping cartons have been specially designed to insure your purchase. However,
damage can occur in any shipment and the following outlines the steps you should take on receipt of a NuAire AIREGARD™
Clean Workstation to be sure that if damage has occurred, the proper claims and actions are taken immediately.

3.1 Damaged Shipments

3.1.1 Terms are factory, unless stated otherwise. Therefore, it is important to check each shipment before acceptance.

3.1.2 If there is visible damage, the material can be accepted after the driver makes a notation on the consignee's copy

of the freight bill. Then an inspection must be made to verify the claim against the carrier. This inspection is the
basis of your filing the claim against the carrier.

3.1.3 If concealed damage is found, it is absolutely necessary to NOTIFY THE FREIGHT AGENT AT ONCE and request an

inspection. Without this inspection, the transportation company may not accept a claim for loss or damage. If the
carrier will not perform the inspection, an affidavit must be prepared stating that he was contacted on a certain
date and that he failed to comply with the request. This, along with other papers in the customer's possession will
support the claim.

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4.0 Installation Instructions

4.1 Location

Within the laboratory, pharmacy, production area, etc., the ideal location for the clean workstation is away from personnel
traffic lanes, air vents (in or out), doors and/or other source of disruptive air currents. If drafts or other disruptive air
currents exceed the face velocity of the HEPA filter, the potential exists for contaminated air to enter the work area of the
workstation. Please note, however, that the work area has been constructed to minimize the effect of air currents by
providing sidewalls that are an extension of the HEPA filter flow area.

Where space permits, a clean six (6) inch (152mm) area should be permitted on each side of the workstation for
maintenance purposes. The electrical outlet into which the workstation is connected should be readily accessible.

The workstation shall be positioned as not to obstruct the power supply outlet or the circuit breaker distribution panel.

4.2 Set-Up Instructions

Remove outer shipping protection (carton or crating). If the workstation is fastened to a base skid, it is usually the best
procedure to leave the skid in place until the workstation is located in its approximate position to facilitate ease in handling.
It can then be removed from the skid by removing the four bolts holding the workstation to the skid.

4.2.1 Motor Shipping Bracket

The motor is securely fastened to the workstation during shipment to prevent damage to the blower housing
mounting brackets. The procedure for removing the motor shipping bracket is shown in Drawing ACD-06899.

4.2.2 Base Stand Assembly

The base stand is shipped K.D. in a separate carton and is assembled per the Drawing accompanied with the unit.
With a forklift or other suitable lifting device, lift the NU-140E between the top of the skid and the bottom of the
workstation, slightly off of the floor and remove the four bolts holding the skid to the workstation (one in each
corner). Now lift the workstation on top of the base and bolt the base stand to the workstation using four 4/20 x
¾” (18mm) bolts and washers provided. Place the workstation in its desired location.

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4.2.3 Gas Service

CAUTION

!

CAUTION

!

NuAire doesn't recommend the use of natural gas within the clean bench, but if gas service is determined to be
necessary for the application by the appropriate safety personnel, appropriate safety measures must take place.

Once the determination has been made by the appropriate safety personnel, the application of natural gas must
be performed in accordance to national, state and local codes.

All NuAire clean benches meet the safety requirements of UL and CSA for Laboratory Equipment. To comply with
these safety requirements, NuAire uses only certified gas valves. In addition, if external piping is required, only
black pipe is used for this application.

As previously stated NuAire doesn't recommend the use of natural gas within the clean bench and ASSUMES NO
RESPONSIBILITY FOR ITS USE. USE AT YOUR OWN RISK. The Bunsen burner flame within the clean bench disrupts
the laminar air stream, which must be maintained for maximum efficiency.

During use, the Bunsen burner should be placed to the rear of the workspace where resulting air turbulence will
have a minimal effect.

4.2.4 Plumbing Services

Ground key cocks with the type of service specified by the snap-in button on the handle, are located in the work
zone. The ground key cocks are not recommended for pressures over 30 p.s.i. Reducing valves should be installed
external to the workstation if necessary. Ground key cocks should never be used for oxygen service. A special
needle valve for oxygen service is required and available upon request.

External connection is to 3/8 inch (10mm) FPT coupling through the sidewalls. Connection to plant utilities should
be made with proper materials for the individual service and according to national and/or local codes. Observe all
labels pertaining to the type of service and operating pressure.

IT IS ALSO STRONGLY RECOMMENDED THAT AN
EMERGENCY GAS SHUTOFF VALVE BE PLACED JUST
OUTSIDE THE CLEAN BENCH ON THE GAS SUPPLY LINE.

IF THE PROCEDURE DEMANDS USE OF A FLAME, A BUNSEN BURNER
WITH ON DEMAND IGNITION IS STRONGLY RECOMMENDED.
DO NOT USE CONSTANT FLAME GAS BURNERS.

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4.2.5 Electrical Services

NOTE:



NOTE:



NOTE:



CAUTION

!

The Vertical Laminar Flow Clean Workstation may be "hardwired" (optional) or connected via an electrical power
cord, which is standard. The unit requires 230 VAC, 50/60 Hz, single phase. (Current rating varies per workstation
size, reference Electrical/Environmental Requirements).

THIS UNIT CONTAINS ELECTRONIC BALLASTS FOR THE FLUORESCENT LIGHTING. ELECTRONIC BALLASTS
OPERATE WITH HIGH INRUSH CURRENT. IT IS NOT RECOMMENDED TO USE THIS PRODUCT WITH
GROUND FAULT CIRCUIT INTERRUPTERS (GFCI'S) BECAUSE THE BALLASTS MAY CAUSE THE GFCI TO TRIP.

If a "hardwired" (conduit) connection is desired, an electrical junction box is provided on the top of the
workstation with a removable cover. All wiring connections to the junction box should be done according to the
National Electrical Code and/or local codes by a qualified electrician.

4.2.6 Final Assembly

The exterior surfaces and viewing glass are easily cleaned with any mild household detergent cleaner using a soft
cloth. Harsh chemicals, solvent-type cleaners and abrasive cleaners should not be used. See section 5.4 for
cleaning procedures. Turn the workstation on and let it operate for 5 minutes before using it as a clean bench.

4.3 Certification Testing Methods and Equipment

After installation and prior to use, NuAire recommends that the workstation be recertified to factory standards.
At a minimum, the following tests should be performed.

1. HEPA filter media

2. Filter frame leak test

3. Airflow velocities

The testing methods and equipment required are specified on the factory inspection report included with this manual.

IT IS RECOMMENDED THAT THESE TESTS BE PERFORMED BY A QUALIFIED TECHNICIAN WHO IS FAMILIAR WITH
THE METHODS AND PROCEDURES FOR CERTIFYING CLEAN BENCHES.

AFTER THE INITIAL CERTIFICATION, NUAIRE RECOMMENDS THAT THE WORKSTATION BE RECERTIFIED AT A
MINIMUM ON AN ANNUAL BASIS AND AFTER EVERY FILTER CHANGE OR MAINTENANCE ACTION OR ANY TIME
THE OPERATOR FEELS IT IS NECESSARY.

Note that the NuAire Clean Workstations, filters and seals provide premium performance; Quality control in both design
and manufacturing insure superior reliability. However, protection to the product is so important, that certification to the
performance requirements should be accomplished as stated to insure conformance to factory standards.

It is recommended that power to each work station (power is defined by
each work station’s power cord), whether individual or joined work
stations, be on its own branch circuit, protected with a circuit breaker at
the distribution panel.

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AIREGARD™ ES Energy Saver Vertical Laminar Flow Work station

Catalog Number

Catalog Number

NU-140-330E

Nominal 3 foot (0.9m)

NU-140-430E

Nominal 4 foot (1.2m)

NU-140-530E

Nominal 5 foot (1.5m)

NU-140-630E

Nominal 6 foot (1.8m)

Performance Specifications

1. Product Protection

IEST-RP-CC002.4
ISO 14644-1

IEST-RP-CC002.4
ISO 14644-1

IEST-RP-CC002.4
ISO 14644-1

IEST-RP-CC002.4
ISO 14644-1

ISO 14644-1

ISO 5

ISO 5

ISO 5

ISO 5

Bench top/console

Work station

Bench top/console

Work station

Bench top/console

Work station

Bench top/console

Work station

16/18 GA, powder

work zone

16/18 GA, powder

work zone

16/18 GA, powder

work zone

16/18 GA, powder

work zone

Diffuser for Air Supply (Metal)

Non-flammable

Non-flammable

Non-flammable

Non-flammable

HEPA Filter Seal Type:

Eff. on 0.3 microns

Optional Services, (Total) Position:

worksurface

under worksurface

worksurface

worksurface

Work Station Size Inches (mm):

Depth

32 (813)

32 (813)

32 (813)

32 (813)

Work Zone Inches (mm):

Depth (at window access height)

24 7/8 (630)

24 7/8 (630)

24 7/8 (630)

24 7/8 (630)

Airflow Volume at 90 fpm (.46 mps)
CFM (CMH) #^

503 (855)

681 (1158)

859 (1460)

1037 (1763)

Heat Rejected, BTU, Per Hour

863

1256

1648

1962

Electrical:

12 ft. Power Cord (one)

CE

14 GA - 3 Wire, 15A

CE

14 GA-3 Wire, 15A

CE

14 GA-3 Wire, 15A

CE

14 GA-3 Wire, 15A

Work Station Weights:***

Net Weight

300 lbs. /136 kg.

355 lbs. /161 kg.

420 lbs. /191 kg.

595 lbs. /270 kg.

Models NU-140-330E/430E/530E/630E

Style of Work station

Work Station Construction

Supply Filter-99.99%

Service Coupling
(3/8 inch NPT)
Gas Valve/Service Coupling
(3/8inch NPT)
Outlet

Height
Width

Height
Width
Depth (on work surface)

w/base stand/storage

coated steel exterior;
16/18 GA type 304 SST

HEPEX Seal- Neoprene,
Spring-loaded

(3) Right/left sidewalls

(3) Right/left sidewalls

(1) Right/left area under

58 (1473)
38 (965)

30 1/8 (765)
33 7/8 (860)
26 3/4 (679)

w/base stand/storage

coated steel exterior;
16/18 GA type 304 SST

HEPEX Seal- Neoprene,
Spring-loaded

(3) Right/left sidewalls

(3) Right/left sidewalls

(1) Right/left area

58 (1473)
50 (1270)

30 1/8 (765)
45 7/8 (1165)
26 3/4 (679)

w/base stand/storage

coated steel exterior;
16/18 GA type 304 SST

HEPEX Seal- Neoprene,
Spring-loaded

(3) Right/left sidewalls

(3) Right/left sidewalls

(1) Right/left area under

58 (1473)
62 (1575)

30 1/8 (765)
57 7/8 (1470)
26 3/4 (679)

w/base stand/storage

coated steel exterior;
16/18 GA type 304 SST

HEPEX Seal- Neoprene,
Spring-loaded

(3) Right/left sidewalls

(3) Right/left sidewalls

(1) Right/left area under

58 (1473)
74 (1880)

30 1/8 (765)
69 7/8 (1775)
26 3/4 (679)

Volts, AC 50/60 Hz
+Amps: Blower/Lights
Rated Amps:

Crated Shipping Weight

# 10% of this value is recirculated
^ Measured at 6” below diffuser screen
*** Crated shipping weight does not include weight for accessories or options
+ Based on Work station with new filters running at 230VAC.

230

1.1
5

340 lbs. /154 kg.

230

1.6
6

405 lbs. /184 kg.

230

2.1
6

470 lbs. /213 kg.

230

2.1
6

565 lbs. /256 kg.

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5.0 Operating the NU-140E

5.1 Aeromax™ Control System

5.1.1 Overview

The Aeromax™ control system is designed to service the control requirements of the AireGard™ ES NU-140E. The
Aeromax™ control system consists of an electronic module that will perform the following functions:

• Easy user interface via LED’s and function keys

• Control blower via solid state switch.

• Control lights via solid state switch.

• Disable audible alarm switch with ring back function.

• Control blower DC ECM motor/blower with solid-state DC Motor Controller that provides automatic

compensation for line voltage variances.

• Monitor and display airflow system performance via PresurFlow™ monitor.

The AireGard™ ES NU-140E offers the latest digital microprocessor design technology for improved performance
and safety. The Aeromax™ control system integrates a digital pressure sensor (PresurFlow™) to monitor the
airflow performance. The Aeromax™ control system also integrates a DC ECM motor/blower controller that
provides automatic compensation for both filter loading and line voltage variances. There is additional on/off
control of blower and light. All the above functions are shown in a system block diagram (see figure 1).

Figure 1

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5.1.2 Front Panel

The control system front panel contains the following functions described in detail (see Drawing BCD-16521).

5.1.2.1 Blower Keys

The blower key controls the ON/OFF power to the blower.
LED above key indicates: full green for blower on,
blinking green for blower pending and
full red for blower alarm.

5.1.2.2 Hidden Key

The hidden key is located just above the blower LED indicator centered in the airflow symbol. The hidden key

is used for various functions including the blower password 3 key sequence if the option is activated.

5.1.2.3 Fluorescent Light Key

The fluorescent light key controls the on/off power to the fluorescent light.

LED above the key indicates full blue for fluorescent light on.

5.1.2.4 Ultraviolet (UV) Light Key (optional)

The UV light key controls the on/off power to the UV light.

LED above indicates full yellow for UV light on.

5.1.2.5 Outlet Keys (only used for service/diagnostics)

The outlet key controls the ON/OFF power to the outlets.
LED above indicates full blue for outlets on.

5.1.2.6 Red Alarm LED

The red alarm LED will indicate any alarm condition and remain indicating until the alarm condition is cleared.

5.1.2.7 Audible Alarm Silence

The audible alarm silence key allows user interaction to silence an audible alarm for a period of 15 minutes.

After 15 minutes if the alarm condition still exists, the audible alarm will again sound.

The audible alarm silence key also is used to exit all Aeromax™ user interaction menus.

5.1.2.8 Arrow Adjustment Keys

The arrow adjustment keys allow user interaction for various functions.

5.1.3 Aeromax™ Control System Power

After the AireGard™ ES NU-140E is plugged into the appropriate facility line power the control system will power
up. The control panel will also indicate the power up status by blinking the red alarm LED. Pressing any key will
acknowledge the power up status and turn off the blinking red alarm LED.

If a power interruption occurs, all control system functions, calibrations and parameters will be maintained and
continue upon restoration of power. Just as the initial power up, the red alarm LED will blink to indicate power up
status.

5.1.4 Standby Mode

When the NU-140E is not in use any of the function keys except the blower that initiates run mode may be turned
on and off in standby mode.

5.1.5 Run Mode

Any time the blower run key is pressed with the hinged window at its correct operational height, the RUN MODE
screen will be initiated. The Run Mode will start with the PresurFlow™ entering and approximate 3 minute warm
up period. The PresurFlow™ LED indicators will blink and indicate the following sequence:

• 1st minute – Left and right Red LED’s will blink

• 2nd minute – Left and right Green LED’s will blink

• 3rd minute – Center 3 Green LED’s will blink

Once the warm up period is complete, only one LED will indicate cabinet airflow status.

During the warm up period the cleaning process may begin.

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

5.1.6 Standby/Run Mode Alarms

If present, standby/run mode alarms will be both visual and audible, the red alarm LED oval will turn on. Audible
alarms will produce an alarm tone for 30 seconds, then ring back for 2 seconds of every 5 seconds. Pressing the
alarm silence key will silence the audible alarm for 15 minutes initially then will start the ring back function again.

The list below represents alarm types and their respective priority from the highest to lowest priority.

1) New Firmware Loaded

2) Internal Board Failure

3) Power on Reset

4) Airflow Pressure Alarm

5) Blower RPM Failure

Note: The above messages are described in greater detail in section 7.

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5.1.7 Operator Accessible Functions



5.1.7.1 Access and Navigation
To access the operator accessible functions,

• Press and hold the key, then enter the 3 key sequence for the desired function, then release

The key and follow each instruction set.

Note: Pressing the key at any time will abort and exit the process without saving any changes
made. Pressing the hidden key will accept all changes and exit.

5.1.7.2 Auto Timer Duration

Auto timer duration timers are countdown timers for the functions displayed once time is entered into a
function. The timer will begin to countdown upon the start of that function (i.e. press light key to start timing
the light). The LED indicator above the function key will start to blink indicating the timer function. If the LED
indicator was full on, no timer function is present. As the timer expires the function will turn off.

• Select auto timer duration function
o Outlets (Special option feature only)

Press and hold key, then press hidden – outlet – outlet keys sequentially.

LED indicator above outlet will blink fast. Adjust desired time as described below.

o Lights

Press and hold key, then press hidden – light – light keys sequentially.
LED indicator above light will blink fast. Adjust desired time as described below.

o UV Light (Diagnostic key use only)

Press and Hold key, then press hidden – UV light – UV light keys sequentially.
LED indicator above UV light will blink fast.
Adjust desired time as described below.

• Low Flow Blower (Diagnostic key use only)

Press and hold key, then press hidden-blower-blower keys sequentially.
LED indicator above Blower key will blink fast. Adjust desired time as described below.

o Adjust countdown time

Press ↑ or ↓ keys to adjust time.
Time will change in 15 minute increments as shown on the PresurFlow™ LED segments
below.

15 minutes for Red LED 30 Minutes for Yellow LED

1 hour for green LED Represents 8 hours (maximum time)

• Press hidden key to accept time and exit.

• Press key at any time to abort and exit.

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5.1.7.3 Blower Password



The blower on/off password allows the cabinet user to place a 3 key sequence requirement to turn the blower
on or off.

The 3 key sequence for the blower password will be a combination of the hidden and blower keys.

• Select blower password

Press and hold key, then press hidden – blower – hidden keys sequentially.
Red LED indicator above blower will blink fast.

• Select password

Press ↑ or ↓ key to scroll through the code choices below,

No Password

B-B-H password

B-H-B password (default)

• Press hidden key to accept time and exit.

• Press key at any time to abort and exit.

Note: If the required blower password option is selected in the blower airflow option menu (see section 7.2.2).
Then the “No password” choice above is not available and the default remains B-H-B.

B-H-H password only

H-B-B password

H-B-H password

H-H-B password

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5.2 Operating Guidelines

Operate the laminar flow workstation continuously. The unit will then remain in its initially clean condition. If, for any
reason, the unit is turned off, turn the unit on and permit to operate for 5 minutes before resuming operations.

Allow only essential items in the work station. Objects should not be placed between the HEPA filter and any point where
the clean environment must be maintained. New items introduced into the work area should be placed downstream of
items already in the work zone for several minutes to allow contaminants to flush off. Note that plastic parts may carry a
static charge which may require special handling in order to remove contaminants.

Particular care must be exercised in placing equipment within the work space. Where possible, equipment should be
placed on perforated platforms to allow air movement under as well as around the object.

All work should be performed with the operator's hand or head downstream of the critical process points. Unnecessary
movement with the work station should be kept to a minimum.

If the workstation is used in a manner not specified by NuAire, the protection provided by the equipment may be impaired.

5.2.1 Operating Sequence

A. Start Up

Turn on workstation blower and lights, check air intake ports of the workstation to make sure they are
unobstructed. The workstations are provided with gauges which indicate pressure differentials across the
filters. They indicate when to replace the filters, dependent upon the blower fan capacity. Blower speed must
only be readjusted by qualified maintenance technicians.

B. Good procedure includes the decontamination or wipe down of workstation surfaces with chemical

disinfectant before work commences.

C. Allow blowers to operate for a minimum of 5 minutes before aseptic manipulations are begun in the

workstation. An additional advantage is obtained from purification (filtration) of the room air circulated
through the equipment. Because of the characteristic contributed to the quality of the laboratory
environment, some owners leave them in operation beyond the time of actual use.

D. Minimize Room Activity - Activity in the room itself should be held to a minimum. Unnecessary activity may

create disruptive air currents, as well as interfere with the work of the operator. A person walking past the
front of the workstation can cause draft velocities up to 175 FPM, which are sufficient to disrupt the air
balance of the Laminar Flow Unit.

E. Utilize Unidirectional Airflow - The operator must keep two important facts in mind:

1) The air, as supplied to the work area through the HEPA filter is contaminant-free.

2) Airborne contamination generated in the work area is controlled by the unidirectional flow of parallel air
streams.
A solid object placed in a laminar air stream will disrupt the parallel flow and consequently, the capability of

controlling lateral movement of airborne particulates. A cone of turbulence extends behind the object and
laminarity of the air stream is not regained until a point is reached downstream, approximately equal to three
to six times the diameter of the object. Within the parameters of this cone, particles may be carried laterally
by multidirectional eddy currents.

5.3 Ergonomics

Ergonomics, the study or accommodation of work practices is extremely important for proper workstation usage and user
health and safety. An evaluation of normal work practices should be performed with each user when working in a

workstation. Evaluation criteria should be at a minimum:
a. Proper user posture
b. Effective workzone layout for work practice
c. Vision or sightlines

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

For each of the above evaluation criterion, several aids may be supplied to accommodate the user.

• Ergonomic chair - A six-way articulating seat and back control for personalized adjustment to assure proper user
posture. Be sure feet are resting on the floor, chair foot support or foot rest. Also be sure back is fully supported with
proper chair adjustments.

• Forearm/elbow support - The workstation is provided with a forearm support on the work access opening. Periodic
mini-breaks during work practice should be taken resting forearm to avoid stress and fatigue. Elbow rests are optional
that can provide support for particular work practices, such as pipetting.

• Effective workzone layout - Always prepare your work procedure to minimize reach to avoid neck and shoulder stress
and fatigue. Rotating tables are optional to maximum workzone and minimize reach.

• Vision and sightline - Always prepare your work procedure to eliminate glare and bright reflections on the window.
Keep your window clean and sightlines clear to your effect workzone.

5.4 Cleaning Procedures

5.4.1 General

Cleaning laboratory equipment is important in terms of both functionality and general good housekeeping. The
information provided below is intended to aid the development of facility Standard Operating Procedures (SOP’s) for
cleaning the equipment. It is strongly recommended that all cleaning materials used be tested and verified in terms of
both effectiveness and material compatibility before they are written into the cleaning SOP documentation.

a. The airflow blower should be operating during the cleaning process to maintain sterility and/or containment during the
cleaning process.

b. Raise window to gain additional access if desired.

c. Apply appropriate cleaning material or surface disinfectant to surfaces. Most surface disinfectants require a specific
contact time depending the materials used within the work zone. CONSULT APPROPRIATE DISINFECTANT
DOCUMENTATION FOR PROPER APPLICATION AND SAFETY PRECAUTIONS.

c-1. Polycarbonate (Covestro® Makrolon®AR) has noted material compatibility concerns (see polycarbonate compatibility
section). They recommend the use of Hydrogen Peroxide based materials such as the following:

It is recommended to AVOID the use of cleaning materials that contain Chlorine, Quaternary Ammoniums and Phenol’s.

If the polycarbonate is lightly scratched, it may be able to be polished out with Mirror Glaze Plastic Polish or similar.

Further information may be available from www.covestro.com

c-2. Stainless steel (type 304) has noted material compatibility concerns with Acids, Chlorides and Halogens. IF THESE

MATERIALS ARE USED AND ALLOWED TO BE LEFT ON THE STAINLESS STEEL SURFACE, OXIDATION AND DEGRADATION
WILL OCCUR. Only by re-wiping surfaces with either sterile water or 70% IPA will remove harmful materials from the

stainless steel surface.

Further information is available at the following: http://www.parrinst.com/wp­content/uploads/downloads/2011/07/Parr_Stainless-Steels-Corrosion-Info.pdf

NOTE: NuAire does not offer any product warranty with respect to cleaning material compatibility. USE AT YOUR OWN
RISK! The information provided above is from raw material suppliers and known general source documents for use to
develop application cleaning SOP’s.

NOTE: When cleaning the work area for the first several times, the new metal surfaces may produce some dark
discoloration on the white cleaning wipes. Repeated cleaning will continuously reduce the amount of the discoloration
material on the cleaning wipes over time.

- Steriplex SD

- Safetec surface wipes

- Peridox RTU

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6.0 General Maintenance



CAUTION

!

CAUTION

!

6.1 Fluorescent/LED Lamp Replacement

6.2 HEPA Filter Replacement

6.3 Pre-Filter Replacement

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All maintenance actions on this equipment must be performed by a qualified
technician who is familiar with the proper maintenance procedures required for this
equipment. This includes both certification as well as repair.

The two (T8) fluorescent bulbs or optional LED lamps are cool white and placed external to the cabinet to aid maintenance
and minimize heat build-up within the cabinet. The life rating of the bulb is 9,000 for fluorescent and 50,000 hours for LED
based on three-hour burning cycles.

To replace a lamp, it is necessary to remove the lamp assembly.

1. Switch cabinet light switch off.

2. Remove the screws at each upper side of the control center and allow the control center to rotate down, resting

on the safety straps.

3. The lamp is now directly exposed for replacement.

4. The lamp is removed by displacing the bulb to one side against the compressible bulb holder and lifting out the

lamp.

5. Reverse the procedure to reinstall the lamp assembly being careful not to pinch the safety straps, cable or tubing

during closure of the control center.

The HEPA filter, under normal usage and barring an accident (puncture), does not need replacement until the efflux velocity
cannot be maintained at 90 LFPM (.457 m/s) + 10%. This may permit the HEPA filter efflux average to be as low as 81 LFPM
(.411 m/s), as long as no point falls below 70 LFPM (.355 m/s). Use only replacement filters of the same rated flow and size
as originally installed to insure proper airflow and HEPA filter lifetime can be achieved.

The HEPA Filter replacement procedure is performed by the following steps: Drawing BCD-19998):

Disconnect electrical power from the cabinet before attempting any maintenance
action.

1. Close the window to fully closed position.

2. Remove flush mount front panel by removing (2) .250 x .500 10-24 shoulder bolts (1 each side top and lower

control box).

3. Remove front pressure panel by removing 8-32 screws along top and bottom of panel.

4. Remove the front screw and tension spring assemblies across the front of the plenum (3 places).

5. Tip the plenum up and hang from hook located center of top header on the top panel.

6. Lift front of HEPA filter and remove.

NOTE: This step usually requires 2 people for the removal and replacement of the HEPA filter.

7. Reverse procedure for installation and operation.

8. Reconnect unit power.

The replacement interval depends on the contaminant (large particles or lint) in the room -- a typical period is every 6
months. The prefilters are located on the top of the workstation.

6.4 Airflow Control System Setup and Calibration

6.4.1 General

The operation of the NU-140E requires that the setup and calibration procedures be performed in order to certify
or commission for usage. The setup and calibration procedures performed ONLY BY THE CERTIFIER ensure that
setpoints are verified and that the airflow monitor sensor is calibrated to the correct values.

6.4.2 Configuration Parameters

Configuration parameters identify NU-140E motor type and size for proper performance characteristics.

• Select/Verify model and size (Bold items represent default parameters)

o Press and hold key, then press blower – blower – blower keys sequentially.

LED indicates above both blower (red) and UV light keys will blink fast.
Review model type/size and change if desired as described below
o Press ↑ or↓ keys to scroll through the LED segment choices associated with model type/size.

Class II, 3 foot

Class II, 4 foot

Class II, 5 foot

Class II, 6 foot

Laminar, 3/4 foot

Laminar, 5/6 foot

o Press hidden key to accept model/size and exit

o Press key at any time to abort and exit

• Select motor control function/type

o Press and hold key, then press light – light – light keys sequentially.

LED indicators above both blower (red) and light keys will blink fast.
Review motor control function/type and change it desired as described below.
o Press ↑ or ↓ keys to scroll through the LED segment choices associated with motor control

function/type.

Auto DC ECM motor

Fixed duty DC ECM motor

Fixed duty EC Impeller motor

o Press hidden key to accept motor control function/type and exit

o Press key at any time to abort and exit.

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6.4.3 Airflow Calibration

CAUTION

!

The NU-140E Airflow Calibration consists of adjusting the airflow.

THIS WORK SHOULD BE DONE ONLY BY A QUALIFIED TECHNICIAN WHO CAN MEASURE THE AIRFLOW WITH A
SUITABLE VELOMETER. NuAire provides one adjustment to set the airflow within the cabinet.

This is: PWM signal adjust via calibration parameter menu.

The PWM signal or blower speed adjustment establishes the NU-140E total volume of airflow as well as makes up
for filter resistance tolerances.

DC ECM motor PWM signal DC voltage should also be monitored and recorded upon final calibration. The DC
voltage may be measured using a digital voltmeter. The two test points to measure DC ECM motor voltage are
located on the DC motor connector on the main control board.

Failure to calibrate airflow to the specified requirements may result
in unsafe conditions of performance (i.e. product protection, noise and
vibration)

BEFORE STARTING AIRFLOW CALIBRATION PROCEDURE, LET THE NU-140E RUN FOR AT LEAST 5 MINUTES.

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The NU-140E is considered to be certifiable if the following airflow measurements are present:
Inflow average: 90 LFPM + 10 LFPM (.46 m/s + 0.05 m/s) using the direct inflow measurement method or related
value using the calculated inflow velocity measurement method.

6.4.3.1 Airflow Calibration





Step 1: Measure the inflow velocity using the recommended procedure found in Table 6.0. If necessary, adjust to

achieve the correct average miniflow velocity within the stated range of 90 + 10 LFPM (.46 + 0.05 m/s).

Step 2: If necessary, enter active blower speed adjustment.

• Press and hold key, then press hidden – blower - ↑ keys sequentially.
LED indicator above blower (green) key will blink fast.

Step 3: Press ↑ or ↓ keys to adjust blower speed.

• LED segments will indicate blower speed percentage and active blower speed adjustment

Note: At any time during the process

6.4.3.2 PresurFlow™ Alarm Set Points

The PresurFlow™ alarm setpoints are based on the calibration setpoint. Once the calibration setpoint is
entered, based on a nominal inflow velocity of 90fpm (.46mls) the associated pressure sensor value is entered
as the nominal pressure value. The high and low alarm setpoints are factory verified and set if needed at
90LFPM (.46 m/s).

However, if specific use alarm setpoints are desired, the alarm setpoints may be adjusted by performing the following: Low Alarm Setpoint

If the low alarm setpoint value is not within an acceptable range, the left end red LED will blink at a very fast rate.

Note: The red LED must be non-blinking to save or exit.

Note: At any time during the process

o Right end red LED indicates active blower speed adjust

The red LED will blink as soon as any adjustments are made and will continue to blink as the
motor rpm settles. Once the red LED stops blinking, the motor will run steady state at the
new percentage.

Note: The red LED must be non-blinking to save or exit

o Green LED’s indicate percentage on of scale (0-100%)
o Yellow LED’s indicate minimum (left/maximum (right) blower speed has been achieved

• Press hidden key to accept and enter the blower speed calibration point

(If the blower speed calibration point was not successfully entered; a half second audible
alarm will occur. The calibration process must then be repeated for successful entry of
blower speed calibration point.)

• Press key to abort and exit

• Press and hold the hidden and ↓ key for 3 seconds.

(The left red LED will blink and the green LED’s indicate blower speed)

• Press ↑ or ↓ keys to adjust blower speed to the desired airflow velocity low alarm setpoint value.

• Left end red LED indicates active low limit blower speed adjust

The red LED will blink as soon as any adjustments are made and will continue to blink as the motor rpm settles. Once the red LED stops blinking, the motor will run steady state ate the new percentage.

• Press hidden key to accept low alarm setpoint value

• Press and hold outlet key for three seconds to remove any previous offsets

• Press key to abort and exit

• Upon exiting, the blower will go back to actual airflows.

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



High Alarm Setpoint

• Press and hold the hidden and ↑ key for 3 seconds. (The right red LED will blink and the green LED’s indicate blower speed)

• Press ↑ or ↓ keys to adjust blower speed to the desired airflow velocity high alarm setpoint value.

• Red end Red LED indicates active high limit blower speed adjust.

The red LED will blink as soon as any adjustments are made and will continue to blink as the motor
rpm settles. Once the red LED stops blinking, the motor will run steady state at the new percentage.
If the high alarm setpoint value is not within an acceptable range, the right end red LED will blink at a
very fast rate.

Note: The red LED must be non-blinking to save or exit.

Note: At any time during the process

• Press hidden key to accept low alarm setpoint value

• Press and hold outlet key for three seconds to remove any previous offsets

• Press key to abort and exit

• Upon exiting, the blower will go back to actual airflows.

Note: Specific use alarm setpoints or the offset pressure value from the nominal calibration point will

be maintained with a new nominal calibration value.

It is not necessary to re-enter the alarm setpoints after a nominal calibration.

6.4.3.3 PresurFlow Alarm Verification

The PresurFlow Alarm setpoints are based on the calibration setpoint. Once the calibration setpoint is

entered, the Alarm setpoint offset pressure values will align from the calibration pressure value. The high
or low alarm setpoint can be verified by measuring inflow volume/velocity while adjusting blower up or
down within the Alarm Verification menu.

• Press and hold key, then press ↑ ↓ ↑ sequentially releasing the key after the 3 key
sequence.

• Note: If blower was off while entering into the Alarm Verification Menu, the low alarm limit will
immediately activate. Turn on blower; once airflow is above the low alarm limit, the alarm will turn
off.

• LED segments will indicate blower speed percentage.

• Press ↑ or ↓ key to raise or lower blower speed. Alarm is active so yellow and red LED’s will activate

if pressure reaches the low or high alarm limit.

• Press to exit (blower should turn off and not go through normal start up procedure).

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Table 6.0

Airflow Velocity Profile

A. Instruments: TSI 8355 Thermo anemometer
B. Procedure:
Air velocity readings are taken on a 12 inch (305mm) grid, in a plane parallel to and 6 inches (152mm) from the diffuser.

No reading should be taken closer than 6 inches (152mm) from the inner edge of the filter frame.

C. Test Data:

Number of Readings: Average Velocity: ft./min. m/s

D. Acceptance Criteria:

1. Average Velocity = 80 to 100 fpm (.41 to .51 m/s)

2. Individual Readings must be within ±20 percent of the average velocity

__________ to __________ fpm (__________ to __________ m/s).

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6.5 HEPA Filter Leak Test

Model Size

Filter Area (ft2)(m2)

330

5.59 (.519)

430

7.57 (.703)

530

9.55 (.887)

630

11.52 (1.07)

To measured 6 inches below the diffuser

Calculated Reference Concentration

NU-140

Airflow, FPM [mps]

Filter Area

80

[0.406]

90

[0.457]

100

[0.508]

Challenge (ug/L)

330

5.59 [.519]

30

27

24

430

7.57 [.703]

22

20

18

530

9.55 [.887]

18

16

14

630

11.52 [1.07]

15

13

12 All calculations use 1 Laskin Nozzle PAO @ 23 PSI

=

=

In order to check filter and filter seal integrity, the HEPA filter media and seals must be directly accessible, by the
measuring instrument. The challenge material (i.e. PAO) should be supplied in the top panel of the workstation. An
upstream challenge may be taken by using the minihelic gauge pressure supply tube if desired.

The diffuser is secured by thumb screws on the sides.
Once removed the diffuser can be dropped down and carefully removed from the cabinet.

Laskin Nozzle Concentration Formula

# Nozzles x 135 CFM x 100 ug/L Challenge
CFM Concentration (ug/L)

# Nozzles x 229 CMH x 100 ug/L Challenge
CMH Concentration (ug/L)

Size

ft2 [m2]

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6.6 Cleanliness Classification Test for Pharmacy Application

If this cabinet is going to be used within pharmacy, per USP7971, the cabinet must be tested to assure compliance to
ISO 14644-1:2015, Cleanrooms and Associated Controlled Environments, Part 1: Classification of Air Cleanliness2. The
cleanliness classification test is performed using a particle counter to measure particle counts within the cabinet workzone.
Turn on cabinet and let warm up for several minutes. Turn on particle counter and flush out sample tubing line to remove
latent particles. Set the particle counter to measure 0.5 micron or larger particles at the appropriate measuring rate.

“Operational Particle Count Test3”

Position the particle counter isokinetic probe at a point 6 inches (152mm) upstream of the aseptic manipulation area (hand
convergence point) and mounted so as not to interfere with the operator’s hand movement. The pharmacy operator will
simulate IV manipulation during the particle count test using non-hazardous materials. A minimum of three (3) 1-minute
particle counts shall be sampled and recorded while the user simulates aseptic compounding manipulations.

“At Rest Particle Count Test”

Take 5 test points in 1-minute intervals on a grid, in a Vertical plane as measured approximately 6-inches (152mm) parallel
to the diffuser. The grid location is designed as the diffuser center point and each corner measured 6-inches (152mm) from
the inside perimeter.

Record the 5 particle count values for each of the test points over the 1-minute sample time. All final count particle
concentrations and calculated 95% upper confidence limit shall not exceed 3520 particles per cubic meter (ppcm) or (100
particles per cubic feet (ppcf).

1

USP28-NF23: United Stated Pharmacopeial Convention, Inc., 12601 Twinbrook Parkway, Rockville, MD

20852, USA, www.usp.org.

2

ISO 14644-1:2015 Cleanrooms and Associated Controlled Environments-Classification of Air Cleanliness, International

Organization for Standardization, Case Postale 56, CH-1211 Geneve 20, Switzerland

3

CAG-002-2006: CETA Compounding Isolator Testing Guide, Controlled Environment Testing Association, 1500 Sunday

Drive, Suite 102, Raleigh, NC 27607, USA, www.cetainternational.org

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6.7 Main Control Board Description and Replacement

OUTLET FUSE
(Option Only)

ACCESSORY OUTPUT FUSE

(Option Only)

CAUTION

!

CAUTION

!

To access the main control board for fuse or board replacement, remove (1) rear screw at each top side of the top panel

and pull out panel to access. Now the main control board is exposed for service.

6.7.1 Main Control Board Replacement

The main control board consists of one Printed Circuit Board (PCB) assembly.
The PCB contains the power supply, configuration switch, sensor inputs/outputs and control inputs/outputs
components and display.

6.7.2 Main Control Board Fuse Replacement

Disconnect electrical power before fuse replacement.

All AC circuits are fuse protected and when replacement is necessary, USE ONLY FUSES OF SAME TYPE AND RATING
FOR PROTECTION AGAINST RISK OF FIRE.

DESCRIPTION: BLOWER FUSE

FUSE TYPE: TIME-LAG TIME-LAG TIME-LAG TIME-LAG

FUSE SIZE: 1/4 X 1-1/4 INCH 5 X 20MM 5 X 20MM 5 X 20MM

NU-140-330E 5 AMPS 3 AMPS 2 AMPS 1 AMP (2)

NU-140-430E 6.25 AMPS 3 AMPS 2 AMPS 1 AMP (2)

NU-140-530E 6.25 AMPS 3 AMPS 2 AMPS 1 AMP (2)

NU-140-630E 6.25 AMPS 3 AMPS 2 AMPS 1 AMP (2)

6.7.3 Main Control Board Replacement

Note: All setup and calibration data will be lost, the memory reinitialized to the default values and all control

functions reset to an initial cabinet power condition.

If possible, before the main control board replacement, it would be preferred to know the operational

parameters of the cabinet, (i.e. blower speed/PWM signal DC voltage setpoints if modified and airflow data
from previous certification).

The main control board is fastened to the control center with (6) 6-32 studs/nuts. All electrical connections are
made with removable terminals and/or Faston connectors except for the motor/blower connector which uses a
screw terminal. Remove all electrical connections and fasteners and then remove the main control board from the
control center.

Install new main control board by reattaching all electrical connections and fasteners. Once installed, rotate
control center to normal position and fasten in place. Reconnect power to cabinet.

LIGHT FUSES

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7.0 Error Messages, Troubleshooting, Option-Diagnostics and Airflow Sensor Performance Verification

Issue

Error Description

Correction

Check light fuse on main control board.

Check ballast.

Check blower fuse on main control board.

board.

Indicates a power interruption has
occurred.

Check sliding window position; should be fully

Check ballast.

UV LED blinks fast and
red LED alarm.

Indicates that the UV light needs
replacement.

Check for short on output of fuse.

AC or DC blower circuit, etc. to isolate the short.

Message acknowledges new

microprocessor

Audible alarms and error messages occur for a variety of reasons. Whenever an alarm condition has been present for a
period of at least 10 seconds, the audible alarm/error message will be presented and stay on until the error is cleared. The
audible alarm will be on for 30 seconds upon initial alarm condition, then once every ten seconds. When presented with an
error message, please perform the following:

Step 1: NOTE ALL ERROR MESSAGES.
Error message will appear on the control panel with red LED’s.
Step 2: VERIFY ERROR MESSAGES.
Error messages can be verified by clearing the error function by either turning the blower or the cabinet on

and off.
Step 3: MONITOR RE-OCCURRENCE OF ERROR MESSAGES.
If re-occurrence of the error message is immediate or daily, use the following guide to correct the situation.

7.1 Error Message Troubleshooting Guide

Check fluorescent lamps.
Cabinet fluorescent
lights won’t Turn on

Cabinet blower won't
turn on.

Blue LED above light key indicates the
lamp should be on.

Green LED above blower key indicates
the blower should be on.
Airflow Alarm.

Check voltage coming out of main control board

to light ballasts.

Check light starters, if present.

Check AC voltage coming out of main control

board.

Check wiring to blower.

Check blower motor.

Check DC motor PWM signal on main control

Red alarm LED blinks

Cabinet ultraviolet light
won’t turn on.

Blower or light fuse
continues to blow after
replacement.

PresurFlow™
left red LED indicator on
and red LED alarm

PresurFlow™
right red LED indicator
on
and red LED alarm

All PresurFlow™
LED’s blink

Yellow LED above UV light key
indicates the UV lamp should be on.

N/A

PresurFlow™ reading low flow
(pressure)

PresurFlow™ reading high flow
(pressure)

firmware was loaded into

Press any key to clear.

closed.

Check blower/lights fuse on main control board.

Check voltage coming out of the main control

board to ultraviolet light ballast.

Replace UV light and clear UV run time clock.

Isolate output of fuse by disconnecting control

center connectors, light circuit,

Check airflow values.

Check blower function.

Recalibrate PresurFlow™ system.

Check airflow values.

Recalibrate PresurFlow™ system.

N/A

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Blower red LED blinks
and red LED alarm

Indicates that the motor rpm signal
has been interrupted

Check connectors and wires from main control

board to the motor

Replace motor if required

7.2 Option Parameters
The option parameter menu allows A QUALIFIED TECHNICIAN to configure several different optional parameters per the

menu as described below.

7.2.1 Sync Function with Active Blower
To access the option parameter menu, perform the following:

• Press and hold key, then press hidden - Blower - Fluorescent keys sequentially.
Red LED indicator above the blower key will blink fast

The PresurFlow™ blinking green LED segments will indicate seven optional parameters as shown and described
below. The UV Light key (move lefts) and outlet key (move right) allows selection of the option parameter desired.

Once the desired option parameter is indicated, press ↑ or ↓ key to turn on or off. A slow blinking green LED
indicator means off and a fast blinking green LED indicator means on. Multiple option changes can be selected.

• Pressing the hidden key will accept all changes and exit

• Pressing the key will abort the process and exit

Sync Fan Relay with Active Blower -
Normally the fan relay will activate when the blower switch is pressed. Blower can
either be actively running or pending. If the fan relay sync is active the blower must
be actively running for the relay to change state.

Sync Accessary Outlet with Active Blower - (only usable if accessory outlet is installed)
Normally the accessary outlet is on all the time. If the accessary outlet sync is active,
the blower must be actively running for the accessary outlet to turn on.

Sync Outlet Power with Active Blower -
Normally the outlet power is turned on via the outlet key. If the outlet power sync is
active, the outlet power will turn on and off with the blower or may be turned on and
off independently if the blower is active.

Sync Fluorescent Light with Active Blower -
Normally the fluorescent light is turned on via the fluorescent light key. If the
fluorescent light sync is active, the fluorescent light will turn on and off with the
blower or may be turned on and off independently if the blower is active.

Sync 15 Volt DC output with active blower normally the 15 Volt DC output located on
the control board is on when power is applied to the system. If the 15 Volt DC output
sync is active, the blower must be actively running for the 15 Volt DC output to turn
on.

7.2.2 Blower/Airflow Options

To access the option parameter menu, perform the following:

• Press and hold key, then press hidden - ↑ and ↓keys sequentially.
Red LED indicator above the blower key will blink fast

The PresurFlow™ blinking green LED segments will indicate seven optional parameters as shown and described
below.
The UV Light key (moves left) and outlet key (moves right) allows selection of the option parameter desired.

Once the desired option parameter is indicated, press ↑ or ↓ key to turn on or off. A slow blinking green LED
indicator means off and a fast blinking green LED indicator means on. Multiple option changes can be selected.

• Pressing the hidden key will accept all changes and exit

• Pressing the key will abort the process and exit

OM0275 Page 38 of 50
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Require Password –



Normally it is not required to use a password (i.e. 3 key press sequence of the blower
and hidden key). If the option is turned on, it would be required to use the correct
password to turn on the blower. The default password once turned on is blower­hidden-blower keys in sequence. The password can be changed in the blower
password option menu.
Note: If turning off the password option, you must also select the no password menu
item (see section 5.1.8.3)

Allow UV light anytime – (only if option is installed)

Normally the UV light is interlocked with the window being in the closed position.
For service purposes only, if the function is active, the UV light may be turned on at
any window height.

Note: In addition to the Allow UV light anytime system function, there is a double redundant UV light
window interlock relay. To override the UV light window interlock relay, the relay itself must
also be shorted. (See electrical schematic for reference).

Manual Blower Restart –

Normally when the blower is actively running and a power interruption occurs.

The blower will automatically come back on when power is restored. If this function
is turned off, the blower will not automatically come back after a power interruption,
but would require the user to press the blower key to restart the blower.

Low Flow –

Normally the function is turned off. If selected and turned on, once the blower is
actively running. Upon closure, the blower will continue to run at a calibrated lower
speed level to maintain a negative airflow. The PresurFlow™ will indicate(s) blinking
green LEDs along with green LED above blower key.

Disable PresurFlow™ -
If this function is active, the digital pressure sensor and alarm function are turned off.

Temporary Low Flow -
When this option parameter is turned on and the other requirements below are met,
the blower key (when held for 5 seconds) will toggle blower between normal and Low
Flow blower speed. The Low Flow blower will time out, based on the Auto Timer
duration for Low Flow without a blower key press and the blower speed will revert
back to normal.

In addition to turning this option on the following requirements must also be met

• Temporary Low Flow Option must be selected

• Password Option must be selected

• Window is at normal height and blower is running

• Low Flow Option must be selected

• Low Flow blower auto timer must be set for a minimum of 15 minutes.

Disable audible alarms / audible key feedback
Normally audible alarms from the PresurFlow™ monitor and audible key feedback are
present to provide an audible sound to the user. If this function is active, all audible
sound will be silenced when the control system is in normal run mode. Audible sound
will still occur in any service/calibration menu function.

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8.0

OM0275 Page 40 of 50
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9.0 Remote Contacts

The NU-140E has several contact closures for remote sensing of various functions.

9.1 Fan Relay

The fan relay contacts are normally open and closed contact closure outputs that are activated whenever the blower key
is pressed and the blower key LED indicator is on or blinking. Contact ratings are 250 VAC maximum at 2 Amps.

9.2 Alarm Relay

The alarm relay contacts are normally open and closed contact closure outputs which are activated whenever an airflow
alarm condition occurs. Contact ratings are 250 VAC maximum at 2 Amps.

9.3 15VDC Output
The 15VDC (100mA) output is generated if the blower is actively running.

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10.0 Optional Equipment

CAUTION

!

10.1 Ultraviolet Light

Personnel should not be present when ultraviolet lamp is on

10.1.1 Overview

The germicidal ultraviolet is primarily intended for the destruction of bacteria and other microorganisms in the air
or on directly exposed surfaces. Approximately 95% of the ultraviolet radiations from germicidal tubes are in the

253.7 nanometer region. This is a region in the ultraviolet spectrum which is near the peak of germicidal
effectiveness. The exposure necessary to kill bacteria is the product of time and intensity. High intensities for a
short period of time, or low intensities for a longer period are fundamentally equal in lethal dosage on bacteria
(disregarding the life cycle of bacteria). The intensity of light falling on a given area is governed by the inverse law;
that is the killing intensity decreases as the distance increases from the tube.

The germicidal tube is placed in the cabinet to provide an average intensity of 100 microwatts per centimeter (for
a new tube) falling on a horizontal plane defined by the bottom of the work surface. The minimum requirement
per paragraph 5.12 of NSF Standard 49 is 40 microwatts per square centimeter (ref. NSF Std. #49, June, 1976).

Since ultraviolet rays will not penetrate ordinary glass, it is recommended that the sliding window be closed while
the ultraviolet light is on within the cabinet; or that personnel leave the cabinet face area.

10.1.2 Operation

The operation of the ultraviolet light is accomplished by closing the sliding window and pressing the UV switch
located on the front panel. The sliding window is interlocked to the ultraviolet light so, when the sliding window is
raised, the ultraviolet light will turn off. If operational time duration is known, the timer can be used in
conjunction with the ultraviolet light to time out the ultraviolet light operation. This can be accomplished by first
turning on the ultraviolet light. Then, set the timer to the desired length of ultraviolet light operation time. Upon
timer expiration, the ultraviolet light will turn off.

10.1.3 Precaution

The rays from germicidal tubes may cause a painful but temporary irritation of the eyes and reddening of the skin,
if of sufficiently high intensity, or if exposure covers a prolonged period of time. For this reason, one should avoid
direct eye and skin exposure to ultraviolet light. If exposure cannot be avoided, it is necessary for personnel to
wear eye goggles or face shields, and long sleeve gowns with rubber gloves.

Since ultraviolet rays will not penetrate ordinary glass, it is recommended that the sliding window be closed while
the ultraviolet light is on within the cabinet; or that personnel leave the cabinet face area.

Ultraviolet light will injure your eyes. Avoid direct viewing at all times.

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10.1.4 Maintenance



The output of an ultraviolet light deteriorates with burning age. The useful life of the light is approximately 5000
hours under specific test conditions.

The control system monitors UV light usage hours and when the 5000 hour point is reached. The UV light LED
indicator will blink fast along with the red LED alarm indicating the UV light should be replaced. To reset the timer,
perform the following:

• Press and hold key the press UV light – UV light – UV light key sequentially and the reset will occur
removing the above alarm condition.

•

It is also possible to approximate the UV light usage by the following:

• Press and hold key and UV light key together for three seconds. The PresurFlow™ LED’s will then
indicate usage of approximately 450 hours per LED light from left to right.

Indicates approximately 450 hours used

Indicates approximately 900 Hours used

Indicates approximately 3600 hours used

Note: Before testing with lamp off, the light may be cleaned with a lint-free cloth dampened with alcohol
or ammonia and water.

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Energies Required to Destroy Some Microorganisms by Ultraviolet Radiation (e)

Microwatt

per cm/2

Microwatt

per cm/2

Penicillium roqueforti

26,400

Paramecium

200,000(a)

Penicillium expansum

22,000

Penicillium digitatum

88,000

Nematode Eggs

40,000(b)

Aspergillus glaucus

88,000

Aspergillus flavus

99,000

Algae

22,000(c)

Aspergillus niger

330,000

Rhizopus nigricans

220,000

Virus

Mucor racemosus A

35,200

Baceriophage (E. Coli)

6,600

Mucor racemosus B

35,200

Tobacco Masaic

440,000

Oospora lactis

11,000

Influenze

3,400(d)

Yeasts

Saccharomyces

13,200

ellipsoideus

17,600

Saccharomyces cerevisiae

13,200

Brewers' yeast

6,600

Baker's yeast

8,800

Common yeast cake

13,200

Bacteria

Streptococcus lactis

8,800

Strep. hermolyticus (alpha type)

5,500

Staphylococcus aureus

6,600

Staphylococcus albus

5,720

Micrococcus sphaeroides

15,400

Sarcina lutea

26,400

Pseudomonas fluorescens

7,040

Escherichia coli

7,040

Proteus vulgaris

7,480

Serratia marcescens

6,160

Bacillus subtilis

11,000

Bacillus subtilis spores

22,000

Spirillum rubrum

6,160

Mold Spores

seconds

Protozoa

seconds

References:

(a) Luckiesh, Matthew (1946) Application of Germicidal, Ethyemal and Infrared Energy,

(b) Hollaender (1942) Aerobiology, A.A.A.S. (for 90% inactivation), pp 162
(c) Ellis, C. and Wells, O.O. (1941) The Chemical Action of Ultraviolet Rays, Reinhold Publishing Corp., pp. 713-714
(d) Hollaender, A., Oliphant, J.W. (1944)
The inactivation effect of monochromatic ultraviolet. Radiation on Influenze Virus (for 90% inactivation) Jour. of Bact. 48, pp. 447-454
(e) This table, "Energies Required to Destroy Some Microorganisms by Ultraviolet Radiation's" comes from Westinghouse brochure
entitled - "Westinghouse Sterilamp Germicidal Ultraviolet Tubes"

OM0275 Page 44 of 50
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D. Van Nostrand o., New York, New York, pp 253

Electrical

Voltage

Phase

Frequency

Current
(Amps)

Independent Outlet Current for

Each Outlet (Optional, Amps)

NU-140-330E

230 Volts

1

50/60 Hz

5

10

NU-140-430E

230 Volts

1

50/60 Hz

6

10

NU-140-530E

230 Volts

1

50/60 Hz

6

10

NU-140-630E

230 Volts

1

50/60 Hz

6

10

WARNING

!

11.0 Electrical/Environmental Requirements

11.1 Electrical (Supply Voltage Fluctuations Not to Exceed +/- 10%)

11.2 Operational Performance (for indoor use only)

Environment Temperature Range: 60°F - 90°F (15.6°C - 32.2°C)

Environment Humidity: Maximum relative humidity 80% for temperatures up to
31°C decreasing linearly to 50% relative humidity at 40°C

Environment Altitude: 6562 Feet (2000 Meters) maximum

11.3 Light Exposure

Standard Fluorescent Lighting @ 150 ft. candles (1614 LUX) maximum intensity.

11.4 Installation Category: 2.0

Installation category (overvoltage category) defines the level of transient overvoltage which the instrument is designed to
withstand safely. It depends on the nature of the electricity supply and its overvoltage protection means. For example,
in CAT II, which is the category used for instruments in installations supplied from a supply comparable to public mains
such as hospital and research laboratories and most industrial laboratories, the expected transient overvoltage is 2500V
for a 230V supply and 1500V for a 120V supply. Main supply fluctuations are not to exceed +10% of nominal voltage.

11.5 Pollution Degree: 2.0

Pollution degree describes the amount of conductive pollution present in the operating environment. Pollution degree 2
assumes that normally only non-conductive pollution such as dust occurs with the exception of occasional conductivity
caused by condensation.

11.6 Chemical Exposure

Chemical exposure should be limited to antibacterial surface disinfectants used for cleaning and disinfecting. USE OF
CHLORINATED OR HALOGEN MATERIALS IN THE CABINET MAY DAMAGE STAINLESS STEEL. Equipment decontamination
can be accomplished by non-condensing gas or vapor Paraformaldehyde, Hydrogen Peroxide or Chlorine Oxide following
NSF/ANSI 49, Annex G.

11.7 EMC Performance (classified for light industrial)

Emissions: EN61326
Immunity: EN61326

The EMC performance requirements are generated within the product
enclosure. The enclosure will be all metal grounded to earth.
In addition, the membrane front panel will also include a ground plane for
maximum protection and an electrostatic shield.

OM0275 Page 45 of 50
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12.0 Disposal and Recycle

Component

Material

Main Workstation

Painted Steel

Worksurface

PVC / Stainless Steel

Side Panels

Polycarbonate / Stainless Steel

HEPA Filter Frame

Aluminum

Control Center

Painted Steel

Diffuser

Aluminum

HEPA Filter

Aluminum

Hepex Bag

PVC

Blower Wheel & Housing

Steel

Motor

Various Steel / Copper

Printed Wiring Assembly

Lead Free Electronic

Wire

PVC Coated Copper

Ballasts

Various Steel, Electronic

Connectors

Nylon

Hardware

Stainless Steel and Steel

NOTE:



RECYCLE



LEAD FREE

Workstations that are no longer in use and are ready for disposal contain reusable materials. ALL components may be
disposed and/or recycled after they are known to be properly disinfected.

Follow all local, state and federal guidelines for disposal of HEPA filter solid waste.

Note: Material type can be verified with use of a magnet with stainless and aluminum being non-magnetic.

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