Nikon Eclipse TI-TIRF-E, Eclipse TI-PAU, Eclipse TI-TIRF, Eclipse Ti, LU4-B5 Setup Manual

M456 E 10.9.NF.5

*M456EN05*

Series

TI-TIRF Illuminator Unit TI-TIRF-E Motorized Illuminator Unit TI-PAU Photo Activation Illuminator Unit

TI-TIRF-PAU Illuminator

LU4-B5 Beamsplitter 50/50

Setup Manual

WARNING

This manual is intended to provide setup instructions for Nikon

representatives who have attended the lecture on laser safety and have been

trained in setup operations. Attempts by others to set up the product may

lead to accidents or equipment failure. NIKON is not responsible for personal

injury or equipment damage resulting from equipment setup performed by

unqualified persons.

Be sure to observe the warnings and cautions described in this manual to

ensure safety during setup.

Introduction

This setup manual is written for service personnel who is installing the Nikon TIRF Illuminator Unit, Motorized TIRF Illuminator Unit, Photo Activation Illuminator Unit, TIRF-PAU Illuminator, and Beamsplitter 50/50 for Ti Series Inverted Microscopes.

To ensure correct installation, read this manual carefully before installing the product.

• No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon.

• The contents of this manual are subject to change without notice.

• This manual contains confidential information. Do not disclose this manual to a third party other than the authorized Nikon personnel.

• Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may remain. If you note any points that are unclear or incorrect, please contact a Nikon Service Department.

• Some of the equipment described in this manual may not be included in the set you are installing.

• If you intend to use any other equipment with this product, read the manual for that equipment too.

• If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

1

Safety Precautions

To ensure correct and safe operation, read this manual before using the product.

WARNING and CAUTION Symbols Used in This Manual Although this product is designed and manufactured to be completely safe during use, incorrect usage or failure to follow the safety instructions provided may cause personal injury or property damage. To ensure correct installation, read the setup manual carefully and thoroughly before installing the product.

Safety instructions in this manual are marked with the following symbols to highlight their importance. For your safety, always follow the instructions marked with these symbols.

Symbol Description

WARNING

CAUTION

All procedures should be performed safely and in accordance with the instructions in this manual to prevent accidents or equipment failures.

Disregarding instructions marked with this symbol may lead to serious injury or death.

Disregarding instructions marked with this symbol may lead to injury or property damage.

2

Safety Precautions

Meaning of the Symbols Used on the Product

The symbols marked on the product indicate the need for caution at all times during use. Always refer to the instruction manual and read the relevant instructions before manipulating any part to which the symbol is marked.

Symbol Meaning

Warning label - laser hazard symbol

This symbol is displayed on the laser safety cover or on the stage to warn that the product emits laser light.

Your eyes or skin may be exposed to the laser light emitted from the objective, resulting in injury.

Precautions against heat

This symbol, affixed to the top of the dia pillar illuminator, the rear side of the 12V-100W lamp house, the HG fiber light source, and the rear side of the super-high pressure mercury lamp (or the xenon lamp) house, calls your attention.

• The lamp and the lamphouse become hot while the lamp is lit and immediately after it is turned off.

• Do not touch the lamp and the lamphouse while the lamp is on and immediately after it is turned off to prevent the risk of burns.

• Make sure that the lamp and the lamphouse are sufficiently cool before the lamp replacement.

Biohazard

This symbol on the upper part of the microscope calls your attention on the following:

• If a specimen is spilled onto the product, it may cause the danger of biohazard.

• To avoid biohazard contamination, do not touch the contaminated portion with your bare hands.

• Decontaminate the contaminated portion according to the standard procedure of your facility.

General attention

This symbol is displayed on the top of the protection plate (for nosepiece) appended to the Ti-E and Ti-E/B and the protection plate (for PFS6 nosepiece) appended to the TI-ND6-PFS motorized PFS6 nosepiece. It calls your attention on the following:

• To avoid accidents such as finger pinching and exposure to laser radiation, install this product in the correct position.

3

Safety Precautions

WARNING

Use of controls or adjustments or performance of procedures other than those specified in this manual may result in hazardous radiation exposure.

1. Laser class

This product is a laser apparatus that uses Class 3B lasers (air-cooled Ar lasers, He-Ne lasers,

semiconductor lasers). Accidental exposure of eyes or skin to laser light may result in injury or other problems. Confirm that there is no one in the area before turning on the lasers. Always wear laser-protection goggles when using the laser.

2. Laser safety

This product is designed and manufactured in compliance with the Performance Standards for

Light-Emitting Products established by the U.S. FDA and the IEC Laser Product Safety Standard (IEC 60825-1), except for deviations pursuant to Laser Notice No.50. Those using this laser product must complete laser safety courses and setup training. Users are requested to take all appropriate safety measures specified by the preceding standards, in accordance with local laws and regulations.

The laser safety officer (LSO) must take charge of this laser product and users should follow the

instructions from the LSO.

FDA Class 3B Laser Product (Laser Notice No. 50)

IEC Class 3B Laser Product

LASER RADIATION AVOID EXPOSURE TO BEAM CLASS 3B LASER PRODUCT

Wavelength: 400 to 700 nm (CW)

Beam divergence: 0.01 to 2.91 rad

Maximum power or energy output: total power 500 mW Max. (CW)

3. Manuals and accessories

All manuals and accessories used in this laser product and provided by the distributor must be

provided to the user.

4. Intended application of the product

This product can be used only for microscopic observations. This product is required to be

attached to the specific Nikon microscope, Ti-E, Ti-E/B, Ti-U, Ti-U/B. Do not use this product for other purpose.

5. Setting up the product

Setup of this product must be handled by trained service personnel.

This task should not be performed by anyone other than the trained service personnel. If performed, it may result in improper setup, impairing system performance and exposing the eyes or skin to the laser light, resulting in injury.

4

Safety Precautions

WARNING

6. Prohibition of dismount

This product uses a laser device. Use only in the configuration set up by the Nikon representative.

To ensure safety, never attempt to remove any part of the system. Before beginning operations, confirm that all of the parts listed below are in place. Removal of any following part during use may result in unintended exposure of eyes or skin to laser light.

Make sure that all powers to the laser and entire system are turned off when removing an objective

or a filter cube.

Eyepiece tube, nosepiece, objective, TIRF/motorized TIRF illuminator unit, photo activation illuminator Unit, TIRF-PAU illuminator, filter cube, sage, lamp house, HG fiber adapter, epi-fl filter turret, 3D-STORM port, TV camera, video camera, or all port caps

7. Do not disassemble.

Disassembling this product may result in electric shock or other hazards.

Never attempt to disassemble any piece of equipment included in this product. In particular, disassembling any of the components when the laser is on may cause the laser light to be improperly emitted from this product. If you notice that this product is not functioning properly, immediately stop use, switch off the power switches to all devices, disconnect the power cables from the outlets, and contact your nearest Nikon representative.

8. Pre-operation check items

Before using the system, always confirm the followings.

If the following conditions are not satisfied, laser light may be irradiated from openings or reflected

from reflective objects, and unintended exposure of eyes and skin to laser light may result.

1 A cap, 3D-STORM port, a TV camera, or a video camera is attached to each port.

2 An objective or a cap is attached to each socket of the nosepiece.

3 The optical fiber for laser light is securely fixed.

4 The adapter for the HG fiber light source (or the lamphouse) is attached for epi illumination.

And the lamphouse is attached for the dia illumination.

5 The epi-fl filter turret is attached correctly. (Its cover is attached too.)

6 The field diaphragm unit is attached correctly.

7 The laser safety cover is attached on the stage. (The specimen cover is attached for TI-PAU.)

8 There is no mirror or other highly reflective piece of metal in the area surrounding the lens

aperture.

9. Never attempt to look into the laser light.

When the laser is on, a powerful beam of light is emitted above the stage through the objective.

Never look into this beam or reflected beams. When the laser is on, put on laser safety goggles. To prevent reflection of the laser light emitted from the objectives and resulting inadvertent exposure of eyes or skin to laser light, never place reflective objects on the stage or in the diascopic optical path.

TIRF PA TIRF-PA

• NOHD: 2502 m 8.9 m 1904 m for 1x objective.

• NOHD: 642 m 2.2 m 492 m for 4x objective.

• NOHD: 258 m 0.9 m 198 m for 10x objective.

MPE at the cornea for exposure to laser radiation: 1 W/m

MPE of the skin to laser radiation: 2000 W/m

2

(400 nm to 700 nm CW)

2

(400 nm to 700 nm CW)

5

Safety Precautions

10. Cautions about heat from the light source

The

Also, the

Follow the cautions below to prevent burn injury and fire.

11. Mercury lamp and xenon lamp

The mercury lamp (or the xenon lamp) used with this product requires special handling. To use the

The hazards described above should not pose any danger as long as you heed all of the warnings

WARNING

symbols affixed to the top of the dia pillar illuminator, the rear side of the lamp house, and the HG fiber light source indicate that the relevant lamp and its surrounding area become very hot while the lamp is lit or right after the lamp is turned off.

symbol affixed above the air vent of the HG fiber light source indicates that hot air

blows out of the air vent while the lamp is lit.

• Never touch the lamp, its surrounding parts, and the air vents while the lamp is lit or within

30 minutes after the lamp is turned off.

• Keep hands away from the air vents when the lamp is lit. Do not block the airflow from the air

vents.

• Make sure that the lamp and its surroundings are sufficiently cool before the lamp replacement.

• Do not allow walls, curtains, or papers to contact the product.

• Do not allow cloths, papers, or highly flammable volatile materials, such as gasoline, benzine,

thinner or alcohol, to come near the lamp, its surrounding parts, or the air vents while the lamp is lit or within 30 minutes after the lamp is turned off.

• Do not draw any power cord on the product body.

system safely and correctly, read the warnings below and be aware of potential dangers. Also, carefully read the manuals of the light source (HG fiber light source, super-high pressure mercury lamp power supply, and high-intensity light source) to be used and the manuals published by the lamp manufacturer if provided, and follow their instructions.

1 The mercury lamp (or the xenon lamp) radiates ultraviolet light that is harmful to the eyes and

skin when the lamp is turned on. Direct viewing of light from these lamps may result in blindness.

2 Gas is sealed under very high pressure inside the lamps. The pressure increases when the

lamp is on. If the lamp is scratched, dirty, subjected to high external pressure or physical impact, or used beyond its operational life, the sealed gas may escape or the lamp may burst. This can result in someone inhaling the gas, injuring themselves on the glass, or other accidents.

3 When the lamp is on, the lamp and its surroundings become extremely hot. Touching the

lamp with bare hands could result in burns. Flammable materials placed near the lamp could ignite.

4 Using other than the specified type of lamp could result in an accident, such as a burst.

and cautions in the manuals and use the system only for its intended purpose because safety is a top priority in the design of Nikon products. However, the hazards described above could lead to an accident if you fail to heed all of the warnings and cautions in the manuals, if you strike the system, or if you attempt to disassemble the system. Therefore, always be sure to heed all of the warnings and cautions.

12. Use the ultraviolet light shield

Do not directly see the light irradiated from the objective. Harmful excitation light will be given out

from the objective depending on the excitation method used. When you see the part around the objective, be sure to see through the ultraviolet light shield.

6

Safety Precautions

13. Notes on handling flammable solvent

The following flammable solvents are used with the product:

Never hold a flame near these solvents. To use solvent, read the instructions provided by the

WARNING

• Immersion oil (Nikon Immersion Oil for oil immersion objectives)

• Absolute alcohol (ethyl alcohol or methyl alcohol for cleaning optical parts)

• Petroleum benzine (for wiping the immersion oil)

• Medical alcohol (for disinfecting the microscopy)

manufacturer and handle the solvent correctly and safely. Note the following precautions to use a solvent with the product.

• Keep solvent from the heat of the lamp, the lamphouse, and the power supply device.

• Do not bring solvent near the product or its surroundings when turning on/off the power switch or

plugging/unplugging the power cord.

• Be careful not to spill solvent.

7

Safety Precautions

1. Precautions on power supply ratings and power cords

Before connecting the power cords to wall outlets, check your power-line ratings to make sure that

2. Precautions on connecting cables

Always switch off the power switches to all devices before connecting or disconnecting cables.

3. Emergency stop

In an emergency, turn off all power switches and unplug the power cords.

4. Do not wet the product.

Do not wet nor spill a liquid on the product to avoid malfunction, over-heating, or electric shock. If a

5. Do not place any object on the product.

To prevent deformation, failure, or malfunction, and to avoid injury, never place any objects (heavy

CAUTION

the input voltage and current capacity requirements of the products are sufficient. Using a wrong power cord may result in malfunction, failure, or fire. Always use power cords that satisfy local safety regulations. Always use three-pole grounded outlets.

To prevent wires from breaking, do not pull cables by the sheath; instead, firmly grasp the plug when connecting or disconnecting a cable.

device is subject to contact with water, immediately switch off the power switch to that device and wipe off the water with a dry cloth. If water enters a device, immediately suspend use of the product, unplug the power cord from the outlet, and contact your nearest Nikon representative.

ones in particular) on any part of the system.

8

Safety Precautions

6. Notes on Handling the Product

CAUTION

1. Handle the product carefully.

The product is a precision optical instrument. Handle the product with care to avoid a physical

shock or an impact. Optical-fiber cables, in particular, must not be bent or pulled with excessive force, to prevent failure, malfunction, or wires from breaking.

2. Notes on handling optical parts

Scratches or dirt such as fingerprints on an optical component (such as a lenses or a filter)

will degrade microscope images. Carefully use the optical parts. Do not damage them. If any part becomes dirty, clean it according to the manuals for respective units.

3. Installation location

To ensure safe and reliable operations, check the following conditions when installing this

product:

• To prevent the product from falling and to avoid eventual failure, install each component of the product on a level surface.

• Install the product in a clean place with little dust and dirt. Dust or dirt degrades system performance significantly.

• Use this product at ambient temperatures of between +5 and +35°C and in conditions of humidity of 60% or less (no condensation). Using the product in hot or humid conditions may result in condensation or malfunction.

• Make sure that the product is not subject to strong vibrations, which may degrade image quality. Consult with Nikon representatives about preventive measures you may take when the product is installed.

• To prevent degradation, malfunction, or failure of the laser and the PC, always use power supply that is free from electric noise or sudden voltage fluctuations.

• To prevent failure, do not block or place any obstacles near the cooling fans of the controller and the laser. When the product is installed near a wall, be sure to provide a clearance of 15 cm or more between the wall and the product.

• Install the product at a place where all the power cables can be pulled out easily in an emergency.

4. Cleaning

Use a dry and soft cloth to clean painted, plastic, and printed parts. If necessary, wipe these

parts with a cloth moistened with diluted neutral detergent, then wipe off the moisture with a soft and dry cloth. To prevent discoloration or deformation of painted or plastic parts or removal of the printed letters/figures, do not use organic solvents such as paint thinner or alcohol.

5. Other categories

Be sure to follow the instructions specified in this manual. Failure to do so may result in

impaired performance, malfunction, failure, or unexpected hazards.

9

How to Use This Manual

Configuration of This Manual

This setup manual is made up of two parts. Refer to the part which deals with the product you are setting up.

• Part 1 For Setting Up TI-TIRF, TI-TIRF-E, or TI-PAU

• Part 2 For Setting Up TI-TIRF-PAU and LU4-B5

10

Part 1

For Setting Up TI-TIRF, TI-TIRF-E, or TI-PAU

Series

Setup Manual

System Configurations and Part Names

1.1 Overview

1.1.1 TIRF Illuminator Unit and Ti-U System Overview

TIRF illuminator unit and Ti-U system overview (front view): When used in combination with two-laser or three-laser unit

(Standard configuration to use the TIRF illuminator unit)

(1)

(4)

(2)

(10)

(12)

(3)

* For details, see Section 8.1, “Overall Configuration,” in Chapter 8, “Specifications and Performance.”

(5)

(6)

(1) Power supply devices for the laser devices

(2) Laser unit (C-LU2, C-LU3, C-LU3EX)

(3) PC

(4) Optical fiber

(5) TI-LUSU shutter unit

(6) AC adapter for the shutter unit

(7) TV camera (video camera)

(8) TI-FLC epi-fl filter turret

(9) Power supply device for the diascopic illumination

(10) TIRF illuminator unit

(11) HG fiber light source

(12) Laser safety cover

(13) Interlock cable

CLASS 3B –

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

CAUTION

(13)

Figure 1.1-1

(7)

(8)

(9)

(11)

1-4

Chapter 1 System Configurations and Part Names

1.1 Overview

TIRF illuminator unit and Ti-U system overview (top view): When used in combination with two-laser or three-laser unit

(1)

(2)

(4)

CLASS 3B –

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

CAUTION

INPUT

RS-232C LASER UNIT

12V

SAFETY

1A

LASER SHUTTER

COVER CLOSE

BINO CLOSE

POWER

(3)

Exclusive use for TI-TIRF system Don't use this unit with other systems.

TI-LUSU

(5)

* For details, see Section 8.1, “Overall Configuration,” in Chapter 8, “Specifications and Performance.”

(1) Power supply devices for the laser devices

(2) Laser unit (C-LU2, C-LU3, C-LU3EX)

(3) PC

(4) Optical fiber

(5) TI-LUSU shutter unit

(6) AC adapter for the shutter unit

(7) TV camera (video camera)

(8) TI-FLC epi-fl filter turret

(9) Power supply device for the diascopic illumination

(10) TIRF illuminator unit

(11) HG fiber light source

(12) Laser safety cover

(13) Interlock cable

(13)

(7)

(6)

Figure 1.1-2

(10)

LASERSTRAHLUNG

CLASS 3B

–

KLASSE 3B,

WENN ABDECKUNG GEÖFFNET

NICHT DEM STRAHL AUSSETZEN

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

VORSICHT

CAUTION

SER RADIATIO OID EXPOSU CLASS 3B LASE

AVOID EXPOSURE

LASER LIGHT IS EMITTE FROM

OBJECTIVE APERT

(12)

(11)

(9)

(8)

1-5

Chapter 1 System Configurations and Part Names

(12)

1.1 Overview

TIRF illuminator unit and Ti-U system overview (front view): When used with four-laser module A

(Standard configuration to use the TIRF illuminator unit)

(1)

(4)

(2)

(13)

POWER

L4

L3

L1

L2

(3)

* For details, see Section 8.1, “Overall Configuration,” in Chapter 8, “Specifications and Performance.”

(1) Power supply devices for the laser devices

(2) Laser unit (LU4A)

(3) PC

(4) Optical fiber

(5) TI-LU4SU shutter unit LU4

(6) TV camera (video camera)

(7) TI-FLC epi-fl filter turret

(8) Power supply device for the diascopic illumination

(9) TIRF illuminator unit

(10) HG fiber light source

(11) Laser safety cover

(12) Interlock cable

(13) USB cable

(9) (11)

CLASS 3B –

CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

(5)

Figure 1.1-3

(7)

(6)

(8) (10)

1-6

Chapter 1 System Configurations and Part Names

(12)

1.1 Overview

TIRF illuminator unit and Ti-U system overview (top view): When used with four-laser module A

(Standard configuration to use the TIRF illuminator unit)

(1)

(2)

(9)

(13)

(4)

(3)

TI-LU4SU

* For details, see Section 8.1, “Overall Configuration,” in Chapter 8, “Specifications and Performance.”

(1) Power supply devices for the laser devices

(2) Laser unit (LU4A)

(3) PC

(4) Optical fiber

(5) TI-LU4SU shutter unit LU4

(6) TV camera (video camera)

(7) TI-FLC epi-fl filter turret

(8) Power supply device for the diascopic illumination

(9) TIRF illuminator unit

(10) HG fiber light source

(11) Laser safety cover

(12) Interlock cable

(13) USB cable

CLASS 3B –

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

CAUTION

(6)

LASER EMISSION

POWER

(5)

Figure 1.1-4

LASERSTRAHLUNG

CLASS 3B

–

CAUTION

KLASSE 3B,

WENN ABDECKUNG GEÖFFNET

NICHT DEM STRAHL AUSSETZEN

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

VORSICHT

SER RADIATIO OID EXPOSU CLASS 3B LASE

AVOID EXPOSURE

LASER LIGHT IS EMITTE

OBJECTIVE APERT

FROM

(8)

(11)

(10)

(7)

1-7

Chapter 1 System Configurations and Part Names

1.1 Overview

1.1.2 Motorized TIRF Illuminator Unit and Ti-E System Overview

Motorized TIRF illuminator unit and Ti-E system overview (front view): When used in combination with two-laser or three-laser unit

(Standard configuration to use the motorized TIRF illuminator unit)

(1)

(4)

(2)

CLASS 3B

CLASS 3B –

–

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

CAUTION

(6) (9) (12)

(3)

(5) (7)

* For details, see Section 8.1, “Overall Configuration,” in Chapter 8, “Specifications and Performance.”

(1) Power supply devices for the laser devices

(2) Laser unit (C-LU2, C-LU3, C-LU3EX)

(3) PC

(4) Optical fiber

(5) TI-LUSU shutter unit

(6) AC adapter for the shutter unit

(7) TV camera (video camera)

(8) TI-FLC-E/HQ motor epi-fl filter turret

(9) Power supply device for the diascopic illumination

(10) Hub controller A (with AC adapter)

(11) Motorized TIRF illuminator unit

(12) HG fiber light source

(13) Remote control pad

(14) Laser safety cover

(15) Interlock cable

(16) USB cable

(11)

(15)

Figure 1.1-5

(16)

(14)

(8)

(13)

1-8

Chapter 1 System Configurations and Part Names

1.1 Overview

Motorized TIRF illuminator unit and Ti-E system overview (top view): When used in combination with two-laser or three-laser unit

(Standard configuration to use the motorized TIRF illuminator unit)

(1)

(2)

(3)

(4)

RS-232C LASER UNIT

LASER SHUTTER

Exclusive use for TI-TIRF system Don't use this unit with other systems.

(5)

SAFETY COVER CLOSE

BINO CLOSE

POWER

TI-LUSU

(15)

INPUT 12V

1A

(11)

CLASS 3B CLASS 3B – –

CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

CAUTION

(14)

(7)

(8)

(6)

Figure 1.1-6

(16)

(10)

LASERSTRAHLUNG

LASERSTRAHLUNG CLASS 3B CLASS 3B

–

– – –

CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

VORSICHT

KLASSE 3B,

WENN ABDECKUNG GEÖFFNET

NICHT DEM STRAHL AUSSETZEN

KLASSE 3B,

WENN ABDECKUNG GEÖFFNET

NICHT DEM STRAHL AUSSETZEN

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

VORSICHT

CAUTION

SER RADIATIO

OID EXPOSU

CLASS 3B LASE

AVOID EXPOSURE

LASER LIGHT IS EMITTE

OBJECTIVE APERT

FROM

OBJECTIVE APERT

FROM

(12)

(9)

(13)

* For details, see Section 8.1, “Overall Configuration,” in Chapter 8, “Specifications and Performance.”

(1) Power supply devices for the laser devices

(2) Laser unit (C-LU2, C-LU3, C-LU3EX)

(3) PC

(4) Optical fiber

(5) TI-LUSU shutter unit

(6) AC adapter for the shutter unit

(7) TV camera (video camera)

(8) TI-FLC-E/HQ motor epi-fl filter turret

(9) Power supply device for the diascopic illumination

(10) Hub controller A (with AC adapter)

(11) Motorized TIRF illuminator unit

(12) HG fiber light source

(13) Remote control pad

(14) Laser safety cover

(15) Interlock cable

(16) USB cable

1-9

Chapter 1 System Configurations and Part Names

1.1 Overview

Motorized TIRF illuminator unit and Ti-E system overview (front view): When used with four-laser module A

(Standard configuration to use the motorized TIRF illuminator unit)

(1)

(4)

(2)

(15)

POWER

L4

L3

L1

L2

(14)

(10) (13)

(7)

(5)

(3)

* For details, see Section 8.1, “Overall Configuration,” in Chapter 8, “Specifications and Performance.”

(1) Power supply devices for the laser devices

(2) Laser unit (LU4A)

(3) PC

(4) Optical fiber

(5) TI-LU4SU shutter unit LU4

(6) TV camera (video camera)

(7) TI-FLC-E/HQ motor epi-fl filter turret

(8) Power supply device for the diascopic illumination

(9) Hub controller A (with AC adapter)

(10) Motorized TIRF illuminator unit

(11) HG fiber light source

(12) Remote control pad

(13) Laser safety cover

(14) Interlock cable

(15) USB cable

(6) (8) (11)

(12)

Figure 1.1-7

1-10

Chapter 1 System Configurations and Part Names

(9)

1.1 Overview

Motorized TIRF illuminator unit and Ti-E system overview (top view): When used in combination with four-laser module A

(Standard configuration to use the motorized TIRF illuminator unit)

(1)

(14)

(2)

(15)

(4)

CLASS 3B CLASS 3B – –

CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

CAUTION

(6)

(3)

TI-LU4SU

* For details, see Section 8.1, “Overall Configuration,” in Chapter 8, “Specifications and Performance.”

(1) Power supply devices for the laser devices

(2) Laser unit (LU4A)

(3) PC

(4) Optical fiber

(5) TI-LU4SU shutter unit LU4

(6) TV camera (video camera)

(7) TI-FLC-E/HQ motor epi-fl filter turret

(8) Power supply device for the diascopic illumination

(9) Hub controller A (with AC adapter)

(10) Motorized TIRF illuminator unit

(11) HG fiber light source

(12) Remote control pad

(13) Laser safety cover

(14) Interlock cable

(15) USB cable

(10)

(7)

LASER EMISSION

POWER

(5)

(15)

Figure 1.1-8

(12)

(8)

(11)

1-11

Chapter 1 System Configurations and Part Names

1.1 Overview

1.1.3 Motorized TIRF Illuminator Unit, LU4A, N-STORM, and Ti-E System

Overview

Motorized TIRF illuminator unit, LU4A, N-STORM, and Ti-E system overview (front view)

(Standard configuration to use the Motorized TIRF illuminator unit)

(1)

(4)

(2)

(15)

POWER

L4

L3

L1

L2

(14)

(10)

(20)(19)

(13)

(7)

(15)

(3)

* For details, see Section 8.1, “Overall Configuration,” in Chapter 8, “Specifications and Performance.”

(1) Power supply devices for the laser devices

(2) Laser unit (LU4A)

(3) PC

(4) Optical fiber

(5) TI-LU4SU shutter unit LU4

(6) EM-CCD camera

(7) TI-FLC-E/HQ motor epi-fl filter turret

(8) Power supply device for the diascopic

illumination

(9) Hub controller A (with AC adapter)

(10) Motorized TIRF illuminator unit

(11) HG fiber light source

(12) Remote control pad

(13) Laser safety cover

(6)

(5)

Figure 1.1-9

(17)

(16) (18)

(12)

(8) (11)

(14) Interlock cable

(15) USB cable

(16) 3D-STORM port

(17) DSC support columns (2 pieces)

(18) Ti-E fixing plate

(19) STORM slider

(20) Lambda plate slider

(21) 405-nm laser adapter with ND

(See Figure 1.1-10 for the location.)

(22) 457-nm laser adapter with ND

(See Figure 1.1-10 for the location.)

(23) 561-nm laser adapter with ND

(See Figure 1.1-10 for the location.)

1-12

Chapter 1 System Configurations and Part Names

(9)

1.1 Overview

Motorized TIRF illuminator unit, LU4A, N-STORM, and Ti-E system overview (top view)

(Standard configuration to use the Motorized TIRF illuminator unit)

(1)

(14)

(22)

(2)

transportation.

Fix the laser unit with

four blocks during

(23)

(15)

(3)

* For details, see Section 8.1, “Overall Configuration,” in Chapter 8, “Specifications and Performance.”

(1) Power supply devices for the laser devices

(2) Laser unit (LU4A)

(3) PC

(4) Optical fiber

(5) TI-LU4SU shutter unit LU4

(6) EM-CCD camera

(7) TI-FLC-E/HQ motor epi-fl filter turret

(8) Power supply device for the diascopic

illumination

(9) Hub controller A (with AC adapter)

(10) Motorized TIRF illuminator unit

(11) HG fiber light source

(12) Remote control pad

(21)

(4)

CLASS 3B CLASS 3B – –

CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

CAUTION

(6)

TI-LU4SU

LASER EMISSION

POWER

(17)

(5)

(16) (13) (18)

Figure 1.1-10

(15)

(20)(19)

(10)

(11)

(8)

(12)

(7)

(13) Laser safety cover

(14) Interlock cable

(15) USB cable

(16) 3D-STORM port

(17) DSC support columns (2 pieces)

(18) Ti-E fixing plate

(19) STORM slider

(20) Lambda plate slider

(21) 405-nm laser adapter with ND

(22) 457-nm laser adapter with ND

(23) 561-nm laser adapter with ND

1-13

Chapter 1 System Configurations and Part Names

1.1 Overview

1.1.4 TI-PAU Photo Activation Illuminator Unit and Ti-U System Overview

Photo activation illuminator unit and Ti-U (front view): When used in combination with two-laser or three-laser unit

(Standard configuration to use the photo activation illuminator unit)

(1)

(4)

(2)

(3)

* For details, see Section 8.1, “Overall Configuration,” in Chapter 8, “Specifications and Performance.”

(1) Power supply devices for the laser devices

(2) Laser unit (C-LU2, C-LU3, C-LU3EX)

(3) PC

(4) Optical fiber

(5) TI-LUSU shutter unit

(6) AC adapter for the shutter unit

(7) TV camera (video camera)

(8) TI-FLC epi-fl filter turret

(9) Power supply device for dia illumination

(10) Photo activation illuminator unit

(11) HG fiber light source

(12) Interlock cable

(5)

CLASS 3B –

CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

(6)

Figure 1.1-11

(7)

(12)

(9)

(8)

(10)

(11)

1-14

Chapter 1 System Configurations and Part Names

(12)

1.1 Overview

Photo activation illuminator unit and Ti-U (top view): When used in combination with two-laser or three-laser unit

(Standard configuration to use the photo activation illuminator unit)

(1)

(2)

(4)

CLASS 3B 

CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

(10)

RS-232C LASER UNIT

LASER SHUTTER

(3)

Exclusive use for TI-TIRF system

Don't use this unit with other systems.

* For details, see Section 8.1, “Overall Configuration,” in Chapter 8, “Specifications and Performance.”

(1) Power supply devices for the laser devices

(2) Laser unit (C-LU2, C-LU3, C-LU3EX)

(3) PC

(4) Optical fiber

(5) TI-LUSU shutter unit

(6) AC adapter for the shutter unit

(7) TV camera (video camera)

(8) TI-FLC epi-fl filter turret

(9) Power supply device for dia illumination

(10) Photo activation illuminator unit

(11) HG fiber light source

(12) Interlock cable

(5)

INPUT 12V

SAFETY

1A

COVER CLOSE

BINO CLOSE

POWER

TI-LUSU

(7)

LASER RADIATION AVOID EXPOSURE TO BE CLASS 3B LASER PROD

AVOID EXPOSURE

LASER LIGHT IS EMITTED FROM

OBJECTIVE APERTURE

(6)

Figure 1.1-12

LASERSTRAHLUNG

CLASS 3B



CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

VORSICHT

KLASSE 3B,

WENN ABDECKUNG GEFFNET

NICHT DEM STRAHL AUSSETZEN

(8)

(9)

(11)

1-15

Chapter 1 System Configurations and Part Names

(11)

1.1 Overview

Photo activation illuminator unit and Ti-U (front view): When used with four-laser module A

(Standard configuration to use the photo activation illuminator unit)

(1)

(4)

(12)

(2)

POWER

L3

L1

L4

L2

(3)

Figure 1.1-13

* For details, see Section 8.1, “Overall Configuration,” in Chapter 8, “Specifications and Performance.”

(1) Power supply devices for the laser devices

(2) Laser unit (LU4A)

(3) PC

(4) Optical fiber

(5) TI-LU4SU shutter unit LU4

(6) TV camera (video camera)

(7) TI-FLC epi-fl filter turret

(8) Power supply device for the diascopic illumination

(9) Photo activation illuminator unit

(10) HG fiber light source

(11) Interlock cable

(12) USB cable

(5)

(9)

(7)

CLASS 3B –

CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

(6) (8) (10)

1-16

Chapter 1 System Configurations and Part Names

(11)

1.1 Overview

Photo activation illuminator unit and Ti-U (top view): When used with four-laser module A

(Standard configuration to use the photo activation illuminator unit)

(1)

(2)

(12)

(4)

(3)

TI-LU4SU

Figure 1.1-14

* For details, see Section 8.1, “Overall Configuration,” in Chapter 8, “Specifications and Performance.”

(1) Power supply devices for the laser devices (2) Laser unit (LU4A) (3) PC (4) Optical fiber (5) TI-LU4SU shutter unit LU4 (6) TV camera (video camera) (7) TI-FLC epi-fl filter turret (8) Power supply device for the diascopic illumination (9) Photo activation illuminator unit (10) HG fiber light source (11) Interlock cable (12) USB cable

(5)

LASER EMISSION

POWER

(6)

(9)

HE BEAM

EN OPEN

TO T

CLASS 3B DIATION WH

POSURE

–

TION

ID EX

CAU

LASER RA

AVO

LASERSTRAHLUNG

CLASS 3B

–

CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

VORSICHT

KLASSE 3B,

WENN ABDECKUNG GEÖFFNET

NICHT DEM STRAHL AUSSETZEN

LASER RADIATION AVOID EXPOSURE TO BE CLASS 3B LASER PROD

AVOID EXPOSURE

LASER LIGHT IS EMITTED FROM

OBJECTIVE APERTURE

(10)

(8)

(7)

1-17

Chapter 1 System Configurations and Part Names

*

1

*

1

1.2 Optical Paths of Lasers

1.2.1 Laser Unit

(3)

(2)

(1)

(5)

(6)

(8) (7)

(1) Laser mounting position L1

(See the table below.)

(2) Laser mounting position L2

(See the table below.)

(3) Laser mounting position L3

(See the table below.)

(4) Laser mounting position L4

(See the table below.)

(5) Motorized shutters

(6) ND filters (ND4 and ND8)

Model name

(2)

(7)

(8)

(1)

(5)

(6)

(2)

(3)

(9)

(8)

(7)

C-LU3EX three-laser unit EXC-LU3 three-laser unitC-LU2 two-laser unit LU4A four-laser module A

(1)

(5)

(11)

(12)

(4)

1

(10)

(7)

(3)

(2) (1)

(8)

Figure 1.2-1

(7) Laser coupling mirror

(8) Laser outlet (single-mode optical fiber)

(9) Wavelength selection filter

(optional for C-LU3EX)

(10) AOTF brightness adjustment part

*1

(11)

ND filters (two ND32s)

*1

(12)

ND filters (ND2 and ND4)

*1: Installed only when the N-STORM kit is

mounted

Table 1.2-1

Laser mounting position

L1 L2 L3 L4

(11)

(5)

(7)

(5)

C-LU2 two-laser unit

C-LU3 three-laser unit

C-LU3EX three-laser unit EX

LU4A four-laser module A

405 nm to 514 nm 532 nm to 640 nm — —

405 nm to 445 nm 594 nm to 645 nm

488 nm to 514 nm

532 nm to 561 nm

633 nm

532 nm to 561 nm

633 nm

—

630 nm to 650nm 400 nm to 445 nm 455 nm to 515 nm 540 nm to 595 nm

LU4A four-laser module A

635 nm to 648 nm 400 nm to 445 nm 455 nm to 515 nm 540 nm to 595 nm

(for N-STORM)

1-18

Chapter 1 System Configurations and Part Names

1.2 Optical Paths of Lasers

1.2.2 Microscope Part (TIRF Illuminator Unit and Ti-U System)

TIRF illuminator unit and Ti-U system (top view)

Laser light

CLASS 3B –

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

CAUTION

LASERSTRAHLUNG

CLASS 3B

–

KLASSE 3B,

WENN ABDECKUNG GEÖFFNET

NICHT DEM STRAHL AUSSETZEN

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

VORSICHT

CAUTION

SER RADIATIO OID EXPOSU CLASS 3B LASE

AVOID EXPOSURE

LASER LIGHT IS EMITTE FROM

OBJECTIVE APERT

INPUT

RS-232C LASER UNIT

12V

SAFETY

1A

LASER SHUTTER

COVER CLOSE

BINO CLOSE

POWER

TI-LUSU

Exclusive use for TI-TIRF system

Don't use this unit with other systems.

Figure 1.2-2

TIRF Illuminator Unit and Ti-U System (front left view)

C A U T

IO N

–

L

C

A

L

S

A S

E

S

R R

3

B A D

A

I

A

V

T

O

IO I D

N

E

W

X

H

P

E

O

N

S

O U R

P

E

N T O

T H

V

E

O

B

R

E

S

A

I C

M

H

T

K

– L A

L

S

A

S

E

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3

S

B W

T

,

R

E

A

N

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A

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B

G

D N

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C

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E

G M S

G

T

E

R

Ö

A

F

H

F

L

N

A

E

T U S S

E T Z E

N

TIRF Illuminator Unit

Optical fiber

Laser light

(from the laser unit)

TV camera

N D 2

N

D

8

16

C

A U T I

O N

–

L

C

A

L

S

A

E

S

R

S

R

3

A

B

D

A

IA

V

O

T

I

O

D

N

E

W X P

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S

N

U

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P E T O

T

V

H

O

E

R

B

S

E

I C H

T

K

– L A

L

S

A

S

E

E R

3 W

S

B

T

,

E

R

N

A

N

H

L

A

U

B

N

D I

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C H

K

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D

N

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G

M

S

G

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Ö

A

F

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F

L

N

A

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U

S S E

T Z E N

Figure 1.2-3

E N A M

G

T

M A E

C

B

U D

O

N

T

O

R

I

E

T

P

R A I

U

R

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D

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A

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S

R

P

A

L

X

R

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B

E

3

S

D I

A

S

O

L

S

V

A

L C

X

E

G I

D

L

I

O

R

O

E

V

S

A

M

A

O

L

R

F

AVOID

EXPOSURE

LASER

LIGHT

FROM

IS

THIS

APERTURE

Laser safety cover

T

E R

D

E

U

E

T

R

I

E

U

M

P

E

S

A

O

S I

E

P

V

I

T

H

C E J B

Epi-fl filter turret

EMITTED

C A U

T I O N

–

C L A

L A

S

E

S R R

3

B A D

A

IA

V

T

O

I

O I D

N

E

W

X

H

P

E

O

N S U

O

R

P

E

E N

T

O T

H

V

E

O

B

R

E

S

A I C

M H

T

K

– L A

L A

S

E

R

3

S

B

W

T

,

R

E

A

N

H

N

L U

A

N

B

G

D

N

E I C

C

H

K

T

U

N

D

G

E

M

S

G

T

E

R

Ö

A

F

H

F

L

N E

A

T

U

S S E T

Z E N

ON

OFF

6V30W

12V100W

MAX.

Ti-U microscope body

Optical path

The TV camera can be installed in a side port (right or left port) or the bottom port (only for Ti-U/B). To change the optical path, use the optical path selector knob on the microscope.

Note that the laser shutter automatically closes when the microscope optical path is changed to the eyepiece. It is not possible to observe images obtained by irradiating the laser to the specimen, from the binocular part of the microscope.

1-19

Chapter 1 System Configurations and Part Names

1.2 Optical Paths of Lasers

1.2.3 Microscope Part (Motorized TIRF Illuminator Unit and Ti-E System)

Motorized TIRF illuminator unit and Ti-E system (top view)

Laser light

CLASS 3B CLASS 3B – –

CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

LASERSTRAHLUNG

LASERSTRAHLUNG CLASS 3B CLASS 3B

–

– – –

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

VORSICHT

KLASSE 3B,

WENN ABDECKUNG GEÖFFNET

NICHT DEM STRAHL AUSSETZEN

CAUTION CAUTION

KLASSE 3B,

WENN ABDECKUNG GEÖFFNET

NICHT DEM STRAHL AUSSETZEN

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

VORSICHT

SER RADIATIO

OID EXPOSU

CLASS 3B LASE

AVOID EXPOSURE

LASER LIGHT IS EMITTE

OBJECTIVE APERT

FROM

OBJECTIVE APERT

FROM

INPUT

RS-232C LASER UNIT

12V

SAFETY

1A

LASER SHUTTER

COVER CLOSE

BINO CLOSE

POWER

TI-LUSU

Exclusive use for TI-TIRF system

Don't use this unit with other systems.

Figure 1.2-4

Motorized TIRF illuminator unit and Ti-E system (front left view)

C A U T I

O N

–

L

C

A

L

S

A

E

S S

R

3 B

A

D

A

I A

V

T

O

I

O I D

N

E

W

X

H

P

E

O

N

S

U

O

R

P

E

N T O

T H

V

E

O

B

R

E

S

A

I C

M H T

K

– L A

L

S

A

S

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3

S

B W

T

,

R

E

A

N

H

N

L U

A

N

B

G

D N

E I C

C

H

K

T

U

D

N

E

G M S

G

T

E

R

Ö

A

F

H

F

L

N E

A

T U S S

E T Z E

N

Motorized TIRF

Illuminator Unit

Optical fiber

Laser light

(from the laser unit)

TV camera

N D 2

N

D

8

16

C A

U T I O

N –

L

C

A

L

S

A

E

S

R

S

R

3

A

B

D

A

I

V

A

O

T

I

O

D

N

E

W X P

H

O

E

S

N

U

O

R

P E T O

T

V

H

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E

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B

S

E

I C H

T

K

– L A

L

S

A

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E

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3

W

S

B

T

,

E

R

N

A

N

H

L

A

U

B

N

D

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I

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C H

K

T

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D

N

E

G

M

S

G

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Ö A H

F

L

N

A

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S E T

Z

E

N

Figure 1.2-5

E N A M

T

M A

T

E

C

B

U D

O

N

T

O

R

I

E

T

P

R A I

U

R

S

D

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A

O

S

R

P

A L

X

R

E

B

E

3

S

D I

A

S

O

L

S

V

A

L

C

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R

D

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U

E

T

R

R I E

U

M

P

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T

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C G I

E

D

L

J

I

B

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R

O

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V

S

A

M

A

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L

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A V

O

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L

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A

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I

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E

H

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M

IS

IT

T

A

E

P

D

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R T U R

C

E

A U

T I O N

– C

L

A

L A

S

E

S R R

3

B A D

A

I

A

V

T

O

I

O I D

N

E

W

X

H

P

E

O

N S U

O R

P

E

E N

T

O T

H

V

E

O

B

R

E

S

A I C

M H

T

K

– L A

L A

S

E

R

3

S

B

W

T

,

R

E

A

N

H

N

L U

A

N

B

G

D

N

E I C

C

H

K

T

U

N

D

G

E

M

S

G

T

E

R

Ö

A

F

H

F

L

N E

A

T U S

S E T Z

E

N

Coarse Fine ExFine

Obj.

ON/OF

F

6V30W

12V100W

MAX.

Laser safety cover

Motorized epi-fl filter turret

Ti-E microscope body

RESET

Z

DISPLAY

BRIGHTNESS

RECALL

FOCUS

EYE

MEMORY

ON

PFS

R100

L100

L80

Optical path

The TV camera can be installed in a side port (right or left port) or the bottom port (only for Ti-E/B). To change the optical path, use the optical path selector switch on the microscope.

Note that the laser shutter automatically closes when the microscope optical path is changed to the eyepiece. It is not possible to observe images obtained by irradiating the laser to the specimen, from the binocular part of the microscope.

1-20

Chapter 1 System Configurations and Part Names

1.2 Optical Paths of Lasers

1.2.4 Microscope Part (Motorized TIRF Illuminator Unit, LU4A, N-STORM, and

Ti-E System)

Motorized TIRF Illuminator Unit, LU4A, N-STORM, and Ti-E System (top view)

transportation.

Fix the laser unit with

four blocks during

Laser light

CLASS 3B

CLASS 3B – –

CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

CAUTION

TI-LU4SU

LASER EMISSION

POWER

Figure 1.2-6

Motorized TIRF Illuminator Unit, LU4A, N-STORM, and Ti-E System (front left view)

C A

U T I O N

–

L

C

A

L

S

A

E

S S

R R

3

B A D

A

I A

V

T

O

I

O I D

N E

W

X

H

P

E

O

N

S

U

O

R

P

E

N T O T H

V

E

O

B

R

E

S

A

I C

M

H

T

K

–

L

A

L

S

A

S

E

R

3

S

B

W

T

,

R

E

A

N

H

N

L U

A

N

B

G

D

N

E I C

C

H

K

T

U

D

N

E

G M S

G T

E

R

Ö

A

F

H

F

L

N E

A

T U S

S E T Z

E

N

Motorized TIRF

Illuminator Unit

STORM slider

Optical fiber

Laser light

(from the laser unit)

M

A

T C

BE

U D

N

TO

O

R

I

E

T

R

P A I

U

R S

D

E

A

O

S

R

P

A

X

L

R

B

E E S

3

ID

A

S O

L

S V

A

L

C

E

R

D

E

U

E

T

R

R I E

U

M

P

E

S

A

O

IS

E

P

IV

T

X

T

H

E

C

IG

E

L

J

ID

B

O

R

O

E

V

S

A

M

A

O

L

R

F

C A

U T I O

N –

L

C

A

L

S

A

E

S

R

S

R

3

A

B

D

A

I

V

A

O

T

I

O

D

N

E

W X P

H O

E

S

N

U

O

R

P

E

T

N O T

V

H

O

E

R

B

S

E

I C

A

H

M

T

K

– L A

L S

A S

S

E

E R

3

W

S

B

T

,

E

R

N

A

N

H L

A

U

B

N

D

G

I

E C

C H

K T

U

D

N

E

G

M

S

G

T

E

R

Ö

A

F

H

F

L

N

A

E

U

T S S

E T Z

E

N

AV

O ID

E

L

X

A

P

S

O

E R

SU

L IG

RE

F

H

R

T

O

M

IS

T

E

H

M

IS

IT

T

A

E

P

D E R

T U R

C

E

A

U T I O N

– C

L A

S

E

S R R

3

B A D

A

I A

V

T

O

I

O I D

N E

W

X

H

P

E

O

N

S

U

O

R

P

E

N T O T H

V

E

O

B

R

E

S

A

I C

M

H

T

K

– L A

L

A

S

E

R

3

S

B W

T

,

R

E

A

N

H

N

L U

A

N

B

G

D N

E I C

C

H

K

T

U

D

N

E

G M S

G

T

E

R

Ö

A

F

H

F

L

N

E

A

T U S S

E T Z

E

N

Laser safety cover

Motorized Epi-fl Filter Turret

Ti-E microscope body

EM-CCD camera

3D-STORM port

Figure 1.2-7

The 3D-STORM port can be installed in a side port (right or left port). To change the optical path, use the optical path selector switch on the microscope.

Note that the laser shutter automatically closes when the microscope optical path is changed to the eyepiece. It is not possible to observe images obtained by irradiating the laser to the specimen, from the binocular part of the microscope.

1-21

Chapter 1 System Configurations and Part Names

1.2 Optical Paths of Lasers

1.2.5 Microscope Part (Photo Activation Illuminator Unit and Ti-U System)

TI-PAU photo activation illuminator unit and Ti-U system (top view)

Laser light

CLASS 3B 

CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

LASERSTRAHLUNG

CLASS 3B



CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

VORSICHT

KLASSE 3B,

WENN ABDECKUNG GEFFNET

NICHT DEM STRAHL AUSSETZEN

LASER RADIATION

AVOID EXPOSURE TO BE

CLASS 3B LASER PROD

AVOID EXPOSURE

LASER LIGHT IS EMITTED

FROM

OBJECTIVE APERTURE

INPUT

RS-232C LASER UNIT

12V

SAFETY

1A

LASER SHUTTER

COVER CLOSE

BINO CLOSE

POWER

TI-LUSU

Exclusive use for TI-TIRF system

Don't use this unit with other systems.

Figure 1.2-8

TI-PAU photo activation illuminator unit and Ti-U system

C A U

T I O

N –

L

C

A

L

S

A

E

S S

R

3 B

A

D

A

I A

V

T

O

I

O I D

N E

W

X

H

P

E

O

N S U

O

R

P

E

N T O T

H

V

E

O

B

R

E

S

A

I C

M H T

K

– L A

L

S

A

S

E

R

3

S

B W

T

,

R

E

A

N

H

N

L

U

A

N

B

G

D

N

E I C

C

H

K

T

U

D

N

E

G

M

S

G

T

E

R

Ö

A

F

H

F

L

N E

A

T

U

S S E

T Z E

N

Motorized TIRF

Illuminator Unit

Optical fiber

Laser light

(from the laser unit)

TV camera

N

D

16

8

2

M

A

T

E

C

B

U

D

O

N

T

O

R

I

E

T

P

R A I

U

R

S

D

E

A

O

S

R

P

A L

X

R

E

B

E

3

S

D I

A

S

O

L

S

V

A

L

C

E

TUR

R

ITTED

U

M

PER

E

S

A

O

S I

P

IVE

T

X

T

E

EC

D

LIGH

I

O

R

OBJ

V

SE

A

OM

LA

FR

C A

U T I O N

–

L

C

A

L

S

A

E

S

R

S

R

3

A

B

D

A

I

V

A

O

T

I

O

D

N

E

W X P

H

O

E

S

N

U

O

R

P

E

T

N O T

V

H

O

E

R

B

S

E I C

A

H

M

T

K

– L A

L

S

A

S

E

E R

3

W

S

B

T

,

E

R

N

A

N

H L

A

U

B

N

D

G

I

E C

C

H

K

T

U

D

N

E

G M S

G

T

E

R

Ö

A

F

H

F

L

N

A

E

U

T S S

E T Z E

N

AVO

ID

EXPO

LASER

SU

LIG

RE

FRO

H T

M

IS

TH

EM

IS

APER

TU

Figure 1.2-9

ITTED R

C

E

A U

T I O

N –

C L A

L A

S

E

S

R

3

B A D

A

I A

V

T

O

I

O I D

N

E

W

X

H

P

E

O

N

S

U

O

R

P

E

N T O

T H

V

E

O

B

R

E

S

A I C

M H T

K

–

L A

L

A

S

E

R

3

S

B

W

T

,

R

E

A

N

H

N

L

U

A

N

B

G

D

N

E

I C

C

H

K

T

U N

D

G

E M S

G

T

E

R

Ö

A

F

H

F

L

N

E

A

T

U S S E

T Z E

N

Laser safety cover

Motorized epi-fl filter turret

Ti-E microscope body

ON

OFF

6V30W

12V100W

MAX.

1-22

Chapter 1 System Configurations and Part Names

1.3 Safety Labels and Openings

The following shows the types and locations of the safety labels affixed to each unit picture, as well as the openings provided in the product. The labels to be affixed to the microscope are shipped with the TIRF illuminator unit/motorized TIRF illuminator unit/photo activation illuminator unit, and the laser safety kit. Affix these labels in place when setting up TIRF-PAU. The other labels are already affixed in place when shipped.

(1) IEC warning label -

laser hazard symbol

(2) IEC explanatory label -

Class 3B laser product

LASER RADIATION AVOID EXPOSURE TO BEAM CLASS 3B LASER PRODUCT

(3) Model name and serial

number label TI-TIRF

TI-TIRF-E TI-PAU (4) Safety standard label

TI-LUSU/TI-LU4SU

This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received,

including interference that may cause undesired operation.

This Class A digital apparatus complies with Canadian ICES-003. Cet appareil numérique de la classe A est confirme à la norme NMB-003 du Canada.

MADE IN JAPAN

(5) IEC caution label -

Class 3B area

CAUTION – CLASS 3B LASER RADIATION WHEN OPEN AVOID EXPOSURE TO THE BEAM

–

VORSICHT KLASSE 3B, WENN ABDECKUNG GEÖFFNET NICHT DEM STRAHL AUSSETZEN

LASERSTRAHLUNG

(6) IEC caution label -

Class 3B area

CAUTION

– CLASS 3B

RADIATION WHEN OPEN

LASER AVOI D

EXPOSURE TO THE BEAM

VORSICHT

WENN NICHT

– LASERSTRAHLUNG KLASSE 3B, ABDECKUNG GEÖFFNET DEM STRAHL AUSSETZEN

(7) IEC caution label -

Class 3B area

CAUTION – CLASS 3B LASER RADIATION WHEN OPEN AVOID EXPOSURE TO THE BEAM

VORSICHT KLASSE 3B, WENN ABDECKUNG GEÖFFNET NICHT DEM STRAHL AUSSETZEN

–

LASERSTRAHLUNG

(8) IEC aperture label 1 (9) IEC aperture label 2 (10) TI-TIRF special label

TI-PAU special label

AVOID EXPOSURE

LASER LIGHT IS EMITTED

OBJECTIVE APERTURE

FROM

AVOID EXPOSURE

LASER LIGHT IS EMITTED FROM

THIS APERTURE

Exclusive use for TI-TIRF system

Don't use this unit with other systems.

(11) Caution label (12) IEC caution label - Class 4

area

Exclusive use for TI-PAU system

Don't use this unit with other systems.

1-23

Chapter 1 System Configurations and Part Names

1.3 Safety Labels and Openings

1.3.1 Ti-U Microscope Body (with the TIRF Illuminator Unit)

Be sure to affix each label to the positions indicated in the figure below.

The microscope body has three openings, A to C, as shown below. Near each of the openings, affix the following labels at the positions shown below.

(1) IEC warning label -

laser hazard symbol

(2) IEC explanatory label -

Class 3B laser product

(7) IEC caution label -

Class 3B area

LASER RADIATION AVOID EXPOSURE TO BEAM CLASS 3B LASER PRODUCT

(8) IEC aperture label 1 (9) IEC aperture label 2

AVOID EXPOSURE

LASER LIGHT IS EMITTED

OBJECTIVE APERTURE

FROM

A

(7)

(1)

(2)

AVOID EXPOSURE

LASER LIGHT IS EMITTED

THIS APERTURE

FROM

C A

U T

IO N

–

C L

A S

S

3B L A

S E

R R

A D

IA T

IO

N

W A V

H

O

E

ID

N

O

E

P

X

E

P

N

O S

U R

E

T

O T

H

E B

E

A

M V O

R S

IC

H T

– L

A S

E

K

R

L

S

A

T

S

R

S

A

E

H

3

L

B

U

,

N

G W E

N

N A

B D

E C

K U

N

G

IC

G

H

T

E

D

Ö E M

F

S

N

T

E

R

T

A

H

L

A U

S S

E

T Z

E

N

M

T

A

C

E

U

B

D

O

R

T

N

E

P

IO

R

T

U

E

S

IA

E

S

R

D

O

A

U

D

A

P

L

T

E

X

R

T

B

R

E

T

E

I

E

R

3

P

R

M

E

S

A

ID

U

E

S

E

O

S

A

V

I

V

O

I

L

T

A

P

T

C

H

X

E

G

E

J

I

B

L

O

ID

R

O

E

M

S

V

O

A

R

L

F

(8)

CAUTION – CLASS 3B LASER RADIATION WHEN OPEN AVOID EXPOSURE TO THE BEAM

–

VORSICHT KLASSE 3B, WENN ABDECKUNG GEÖFFNET NICHT DEM STRAHL AUSSETZEN

LASERSTRAHLUNG

C A

U T IO

N

–

C

L A

S S

3 L A

B

S

E R

R A

D

IA

T IO

N

W A V

H

O

E

ID

N

E

O

X

P

E

O

N

S

U

R E

T

O

T

H E

B

E A

M V O R

S IC

H T

–

L

A S

E

K

R

L

A

S

T

S

R

E

A

3

H

L

B

U

,

N

G

W E

N

N A

B D

E

C K

U

N

IC

G

H

T D

E

Ö

M

F F

S

N

T

E

R

T

A

H L

A

U S

S E

T Z

E

N

B

(7)

A

V

O

ID

E

X

P

O

S U

LASER

R

E

LIG

HT

IS

FROM

EM

ITTED

TH

IS

CA

APERTURE

UTION

– CLASS

3B

LASE

R R

ADIATIO

N W

AVO

HEN

ID EXPO

OPEN

SU

RE TO

TH

E BEAM

VORSICHT

–

LASE

KLASSE

RSTRAH

3B,

LUNG

W

E

NN

ABD

EC

KU

NG

NICH

G

T D

EÖ

EM

FFNET

STRA

HL AUSS

ETZE

N

ON

OFF

6V30W

12V100W

MAX.

C

(9) (both sides)

(7) (both sides)

Figure 1.3-1

1-24

Chapter 1 System Configurations and Part Names

1.3 Safety Labels and Openings

1.3.2 Ti-E Microscope Body (with the Motorized TIRF Illuminator Unit)

Be sure to affix each label to the positions indicated in the figure below.

The microscope body has three openings, A to C, as shown below. Near each of the openings, affix the following labels at the positions shown below.

(1) IEC warning label -

laser hazard symbol

(2) IEC explanatory label -

Class 3B laser product

(7) IEC caution label -

Class 3B area

LASER RADIATION AVOID EXPOSURE TO BEAM CLASS 3B LASER PRODUCT

(8) IEC aperture label 1 (9) IEC aperture label 2

AVOID EXPOSURE

LASER LIGHT IS EMITTED

OBJECTIVE APERTURE

FROM

A

(7)

(1)

(2)

AVOID EXPOSURE

LASER LIGHT IS EMITTED

THIS APERTURE

FROM

C

A U

T

IO

N

–

C L

A S

S 3

L

B A S

E

R R

A

D

IA

T IO

N W

A

V

H O

E

ID

N

E

O

P

X

E

P

N

O

S

U R

E T

O

T

H E

B

E A

M

V

O R

S

IC

H T

–

L

A S

E

K

R

L

S

A

T

S

R

S

A

E

H

3

L

B

U

,

N

G

W

E

N N

A

B

D

E C

K U

N

G

IC

G

H T

D

E

Ö

E

M

F

S

N

T

E

R

T

A

H L A

U

S S

E

T Z

E N

(8)

CAUTION – CLASS 3B LASER RADIATION WHEN OPEN AVOID EXPOSURE TO THE BEAM

–

VORSICHT KLASSE 3B, WENN ABDECKUNG GEÖFFNET NICHT DEM STRAHL AUSSETZEN

LASERSTRAHLUNG

M

T

A

C

E

U

B

D

O

T

R

N

P

E

O

I

R

T

U

E

A

S

I

S

E

D

O

A

R

L

A

P

U

D

T

E

X

R

B

R

T

E

3

E

R

IT

R

P

D

M

E

S

A

I

U

E

S

O

E

A

S I

V

O

IV

L

A

T

P

T

C

X

H

E

G

E

J

I

B

L

D

O

I

R

E

O

M

S

V

O

A

R

L

F

C

A

U T

IO N

–

C L A

S

S 3

L

A

B

S

E

R R

A D

IA T

IO

N

W

A

V

H

O

E

ID

N

E

O

X

P

E

O

N S U

R

E

T O

T H

E

B

E A

M

V

O R

S IC

H

T –

L A

S E

K

R L A

S

T

S

R

E

A

H

3

L

B

U

,

N G

W

E N

N A

B

D E

C K U

N

IC

G

H

T D

E

Ö

M

F F

S

N

T

E

R

T

A

H

L A

U

S S

E

T Z

E

N

B

(7)

(9) (both sides)

C

A

V

OID

E

X

P

O

S

LASE

U

R E

R

LIG

HT

IS

FR

E

M

OM

ITTE

TH

D

IS

CAUTIO

A PERTU

N

– C

LASS 3B

R

E

LASER RAD

IA

TIO

N WHEN

AVOID

OPEN

EXPOSUR

E

TO

THE BEAM

VOR

S

IC

HT

– LASER

KLA

S TR

SSE

AH

3B

LU

NG

,

WENN ABDEC

KUNG GEÖFFN

NICHT DEM

STRAHL A

E T

USSETZEN

(7) (both sides)

RESET

-

Coarse Fine ExFine

Obj.

ON/OF

F

6V30W

12V100W

MAX.

Z

DISPLAY

RECALL

BRIGHTNESS

Y

MEMOR

FOCUS

PFS

ON

EYE

R100

L100

L80

Figure 1.3-2

1-25

Chapter 1 System Configurations and Part Names

1.3 Safety Labels and Openings

1.3.3 Ti-U Microscope Body (with the TI-PAU Photo Activation Illuminator Unit)

Be sure to affix each label to the positions indicated in the figure below.

The microscope body has three openings, A to C, as shown below. Near each of the openings, affix the following labels at the positions shown below.

(1) IEC warning label -

laser hazard symbol

(2) IEC explanatory label -

Class 3B laser product

(7) IEC caution label -

Class 3B area

LASER RADIATION AVOID EXPOSURE TO BEAM CLASS 3B LASER PRODUCT

(8) IEC aperture label 1 (9) IEC aperture label 2

AVOID EXPOSURE

LASER LIGHT IS EMITTED FROM

OBJECTIVE APERTURE

AVOID EXPOSURE

LASER LIGHT IS EMITTED

THIS APERTURE

FROM

A

C

A

U T IO

N

–

C

L A

S S

3

L

B

A

S E R

R

A D

IA

T

IO N

W

A

V

H

O

E

ID

N

O

E

P

X

E

P

N

O S

U

R E

T

O T

H

E B

E A

M

V

O

R S IC

H

T –

L A

S E

K

R

L

S

A

T

S

R

S

A

E

H

3

L

B

U

,

N G

W

E

N N

A

B D

E

C K

U N

N

G

IC

H

G

T

D

E

Ö

E

M

F

S

N

T

E

R

T

A

H L

A

U S

S E

T Z E

N

(7)

CAUTION – CLASS 3B LASER RADIATION WHEN OPEN AVOID EXPOSURE TO THE BEAM

–

VORSICHT KLASSE 3B, WENN ABDECKUNG GEÖFFNET NICHT DEM STRAHL AUSSETZEN

LASERSTRAHLUNG

(1)

(8)

(2)

M

T

T A

A

C

C E

E

U

U B

B

D

D O

O

R

R T

T

N

E

P

P IO

IO

R

T

U

E

E S

S

IA

E

S

R

D

O

A

U

U D

D

A

P

L

T

T E

E

X

R

R T

T

B

B R

R

E

T

E

I

R

3

P

R

M

E

S

A

ID

U

E

S

E

O

S

A

V

I

V

O

I

L

T

A

P

T

C

C H

H

X

E

E G

G

E

J

J I

I

B

B L

L

O

ID

R

O

E

M

S

V

O

A

R

R L

L

F

C A

U T IO

C

N

A

U

–

T IO

C

L

N

A

L

–

S

A

S

C

S

3

L

E

A

B

R

S

R

S

3

A

L

D

A

B

IA

S E

T

R

IO

A

R

V

N

O

A

W

D

ID

IA

H

E

T

X

IO

N

P

O

N

O

W

P

S

A

E

V

U

H

N

O

R

E

ID

E

N

T

E

O

X

P

T

P

E

O

H

N

S

E

U

B

R

E

A

T

M

V

O

T

R

H

S

E

IC

B

H

E

T

A

–

M

L

K

A

L

S

A

E

S

R

S

V

S

E

O

T R

R

3

A

B

S

H

IC

,

L

H

W

U

T

N

E

–

N

G

L

N

A

S

B

E

K

D

R L A

E

S

T

C

N

S

R

K

IC

E

A

U

3

H

N

L

T

G

B

U

D

,

N

E

G

M

E

Ö

S

W

T

F

E

R

F

N

A

N

H

E

A

T

L

A

B

D

U

E

S

C

S

K

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T A H L

A U

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(7)

A

V

O

ID

E

X

P

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S

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LASER

R

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LIGHT

IS

FROM

EMITTED

THIS

C AU

APERTURE

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–

C

LA

S S

3B

LA

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W

A

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ID EX

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P

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PO

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T

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EA

M

VO

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SE

K

R

LA

ST

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RA

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3B,

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A

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ON

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6V30W

12V100W

MAX.

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(9) (both sides) (7) (both sides)

Figure 1.3-3

1-26

Chapter 1 System Configurations and Part Names

1.3 Safety Labels and Openings

1.3.4 C-LU2 Two-laser Unit

Make sure that the labels are affixed in place.

(1) IEC warning label -

laser hazard symbol

(6) IEC caution label -

Class 3B area

CAUTION

– CLASS 3B

RADIATION WHEN OPEN

LASER AVOI D

EXPOSURE TO THE BEAM

VORSICHT

WENN NICHT

– LASERSTRAHLUNG KLASSE 3B, ABDECKUNG GEÖFFNET DEM STRAHL AUSSETZEN

(6)

(1)

Figure 1.3-4

1-27

Chapter 1 System Configurations and Part Names

1.3 Safety Labels and Openings

1.3.5 C-LU3 Three-laser Unit

Make sure that the labels are affixed in place.

(1) IEC warning label -

laser hazard symbol

(6) IEC caution label -

Class 3B area

CAUTION

– CLASS 3B

RADIATION WHEN OPEN

LASER AVOI D

EXPOSURE TO THE BEAM

VORSICHT

WENN NICHT

– LASERSTRAHLUNG KLASSE 3B, ABDECKUNG GEÖFFNET DEM STRAHL AUSSETZEN

(6)

(1)

Figure 1.3-5

1-28

Chapter 1 System Configurations and Part Names

1.3 Safety Labels and Openings

1.3.6 C-LU3EX Three-laser Unit EX

Make sure that the labels are affixed in place.

(1) IEC warning label -

laser hazard symbol

(6) IEC caution label -

Class 3B area

CAUTION

– CLASS 3B

RADIATION WHEN OPEN

LASER AVOI D

EXPOSURE TO THE BEAM

VORSICHT

WENN NICHT

– LASERSTRAHLUNG KLASSE 3B, ABDECKUNG GEÖFFNET DEM STRAHL AUSSETZEN

(6)

(1)

Figure 1.3-6

1-29

Chapter 1 System Configurations and Part Names

1.3 Safety Labels and Openings

1.3.7 LU4A Four-laser Module A

Make sure that the labels are affixed in place.

(1) IEC warning label -

laser hazard symbol

(12) IEC caution label - Class 4

area

(12)* (1)

Figure 1.3-7

* For the laser path inside the laser unit, see Section 1.2.1, “Laser Unit.”

1-30

Chapter 1 System Configurations and Part Names

1.3 Safety Labels and Openings

1.3.8 LU-LR Four-laser PS Rack

Make sure that the label is affixed in place.

(11) Caution label

(Front view)

(11)

(Rear view)

Figure 1.3-8

1-31

Chapter 1 System Configurations and Part Names

1.3 Safety Labels and Openings

1.3.9 TI-LUSU Shutter Unit

For the TIRF illuminator unit or the motorized TIRF illuminator unit When used in combination with two-laser or three-laser unit

Make sure that the labels are affixed in place.

(4) Safety standard label (10) TI-TIRF special label

Exclusive use for TI-TIRF system

This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received,

including interference that may cause undesired operation.

This Class A digital apparatus complies with Canadian ICES-003. Cet appareil numérique de la classe A est confirme à la norme NMB-003 du Canada.

Don't use this unit with other systems.

(10)

MADE IN JAPAN

RS-232C LASER UNIT

LASER SHUTTER

Exclusive use for TI-TIRF system

Don't use this unit with other systems.

(Top view)

SAFETY COVER CLOSE

BINO CLOSE

POWER

12V

TI-LUSU

INPUT

1A

(Bottom view)

Figure 1.3-9

1-32

operation.

Cet appareil numérique de la classe A est confirme

à la norme NMB-003 du Canada.

This Class A digital apparatus complies with Canadian

ICES-003.

MADE IN JAPAN

including interference that may cause undesired

This device complies with Part 15 of the FCC Rules.

Operation is subject to the following two

conditions:

(1)this device may not cause harmful interference,

and

(2)this device must accept any interference received,

(4)

Chapter 1 System Configurations and Part Names

1.3 Safety Labels and Openings

For the photo activation illuminator unit When used in combination with two-laser or three-laser unit

Make sure that the labels are affixed in place.

(4) Safety standard label (10) TI-PAU special label

Exclusive use for TI-PAU system

This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received,

including interference that may cause undesired operation.

This Class A digital apparatus complies with Canadian ICES-003. Cet appareil numérique de la classe A est confirme à la norme NMB-003 du Canada.

Don't use this unit with other systems.

(10)

MADE IN JAPAN

RS-232C LASER UNIT

LASER SHUTTER

Exclusive use for TI-PAU system

Don't use this unit with other systems.

(Top view)

SAFETY COVER CLOSE

BINO CLOSE

POWER

12V

TI-LUSU

INPUT

1A

ON

OFF

(Bottom view)

Figure 1.3-10

1-33

operation.

Cet appareil numérique de la classe A est confirme

à la norme NMB-003 du Canada.

This Class A digital apparatus complies with Canadian

ICES-003.

MADE IN JAPAN

including interference that may cause undesired

This device complies with Part 15 of the FCC Rules.

Operation is subject to the following two

conditions:

(1)this device may not cause harmful interference,

and

(2)this device must accept any interference received,

(4)

Chapter 1 System Configurations and Part Names

1.3 Safety Labels and Openings

1.3.10 TI-LU4SU Shutter Unit LU4

For the TIRF illuminator unit or the motorized TIRF illuminator unit When used with four-laser module A

Make sure that the label is affixed in place.

(4) Safety standard label

This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received,

including interference that may cause undesired operation.

This Class A digital apparatus complies with Canadian ICES-003. Cet appareil numérique de la classe A est confirme à la norme NMB-003 du Canada.

MADE IN JAPAN

TI-LU4SU

(Top view)

LASER EMISSION

POWER

(4)

operation.

Cet appareil numérique de la classe A est confirme

This Class A digital apparatus complies with Canadian

ICES-003.

à la norme NMB-003 du Canada.

MADE IN JAPAN

This device complies with Part 15 of the FCC Ru les.

Operation is subject to the following two

conditions:

(1) this dev ice may not cause harmful interference,

and

(2) this dev ice must accept any interference received,

including interference that may cause undesired

(Bottom view)

Figure 1.3-11

1-34

Chapter 1 System Configurations and Part Names

1.3 Safety Labels and Openings

For the photo activation illuminator unit When used with four-laser module A

Make sure that the label is affixed in place.

(4) Safety standard label

This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received,

including interference that may cause undesired operation.

This Class A digital apparatus complies with Canadian ICES-003. Cet appareil numérique de la classe A est confirme à la norme NMB-003 du Canada.

MADE IN JAPAN

TI-LU4SU

LASER EMISSION

POWER

(Top view)

(4)

operation.

Cet appareil numérique de la classe A est confirme

This Class A digital apparatus complies with Canadian

ICES-003.

à la norme NMB-003 du Canada.

MADE IN JAPAN

This device complies with Part 15 of the FCC Ru les.

Operation is subject to the following two

conditions:

(1) this dev ice may not cause harmful interference,

and

(2) this dev ice must accept any interference received,

including interference that may cause undesired

(Bottom view)

Figure 1.3-12

1-35

Chapter 1 System Configurations and Part Names

1.3 Safety Labels and Openings

1.3.11 TI-TIRF TIRF Illuminator Unit

Make sure that the labels are affixed in place.

This system has four openings, A to D, as shown below. Make sure that the following labels are affixed near each of the openings.

(3) Model name and serial

number label

(5) IEC caution label -

Class 3B area

(7) IEC caution label -

Class 3B area

CAUTION – CLASS 3B LASER RADIATION WHEN OPEN AVOID EXPOSURE TO THE BEAM

–

VORSICHT KLASSE 3B, WENN ABDECKUNG GEÖFFNET NICHT DEM STRAHL AUSSETZEN

LASERSTRAHLUNG

CAUTION – CLASS 3B LASER RADIATION WHEN OPEN AVOID EXPOSURE TO THE BEAM

–

VORSICHT KLASSE 3B, WENN ABDECKUNG GEÖFFNET NICHT DEM STRAHL AUSSETZEN

LASERSTRAHLUNG

(5)

M

N

A

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P

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3

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B

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C

Figure 1.3-13

1-36

Chapter 1 System Configurations and Part Names

1.3 Safety Labels and Openings

1.3.12 TI-TIRF-E Motorized TIRF Illuminator Unit ― For Normal Microscopy

(Except N-STORM)

Make sure that the labels are affixed in place.

This system has four openings, A to D, as shown below. Make sure that the following labels are affixed near each of the openings.

(3) Model name and serial

number label

(5) IEC caution label -

Class 3B area

(7) IEC caution label -

Class 3B area

CAUTION – CLASS 3B LASER RADIATION WHEN OPEN AVOID EXPOSURE TO THE BEAM

–

VORSICHT KLASSE 3B, WENN ABDECKUNG GEÖFFNET NICHT DEM STRAHL AUSSETZEN

LASERSTRAHLUNG

CAUTION – CLASS 3B LASER RADIATION WHEN OPEN AVOID EXPOSURE TO THE BEAM

–

VORSICHT KLASSE 3B, WENN ABDECKUNG GEÖFFNET NICHT DEM STRAHL AUSSETZEN

LASERSTRAHLUNG

(3)

Back view

(7)

(7)(7)

(5)

(7)

A

B

C

D

Figure 1.3-14

1-37

Chapter 1 System Configurations and Part Names

1.3 Safety Labels and Openings

1.3.13 TI-TIRF-E Motorized TIRF Illuminator Unit ― For N-STORM Microscopy

Make sure that the labels are affixed in place.

This system has four openings, A to D, as shown below. Make sure that the following labels are affixed near each of the openings.

(3) Model name and serial

number label

(5) IEC caution label -

Class 3B area

(7) IEC caution label -

Class 3B area

CAUTION – CLASS 3B LASER RADIATION WHEN OPEN AVOID EXPOSURE TO THE BEAM

–

VORSICHT KLASSE 3B, WENN ABDECKUNG GEÖFFNET NICHT DEM STRAHL AUSSETZEN

LASERSTRAHLUNG

CAUTION – CLASS 3B LASER RADIATION WHEN OPEN AVOID EXPOSURE TO THE BEAM

–

VORSICHT KLASSE 3B, WENN ABDECKUNG GEÖFFNET NICHT DEM STRAHL AUSSETZEN

LASERSTRAHLUNG

(3)

Back view

(7)(7)

(7)

(5)

(7)

A

B

C

D

Figure 1.3-15

1-38

Chapter 1 System Configurations and Part Names

1.3 Safety Labels and Openings

1.3.14 TI-PAU Photo Activation Illuminator Unit

Make sure that the labels are affixed in place.

This system has four openings, A to D, as shown below. Make sure that the following labels are affixed near each of the openings.

(3) Model name and serial

number label

(5) IEC caution label -

Class 3B area

(7) IEC caution label -

Class 3B area

CAUTION – CLASS 3B LASER RADIATION WHEN OPEN AVOID EXPOSURE TO THE BEAM

–

VORSICHT KLASSE 3B, WENN ABDECKUNG GEÖFFNET NICHT DEM STRAHL AUSSETZEN

LASERSTRAHLUNG

CAUTION – CLASS 3B LASER RADIATION WHEN OPEN AVOID EXPOSURE TO THE BEAM

–

VORSICHT KLASSE 3B, WENN ABDECKUNG GEÖFFNET NICHT DEM STRAHL AUSSETZEN

LASERSTRAHLUNG

(3)

(7)

Back

(7)

(5)

N

T

E

Z

N

T

G

F

E

N

M

F

S

A

U

Ö

N

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A

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CLASS 3B – N

ID EXPOSURE TO THE BEAM

AVO

LASER RADIATIO

CAUTIO

I

N I N

ND2

N

D

16

8

2

VORSICHT

KLASSE

WENN

NICHT DEM STRAHL AUSSETZEN

ABDECKUNG GEÖFFNET

AVO

3B,

–

LASERSTRAHLUNG

LASER RADIA ID EXPOSURE

CAUTION

– CLASS 3B TION WHEN OPEN

TO THE BEAM

C A

U T

IO

LASER RAD

N

–

C

AVO

L

A

ID EXPOSURE

IATION W

S

S 3

B

HEN OPEN

V

O R

TO T

S

K

I

L

C

A

W

H

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S

T

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S

N

–

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I

N

C

L

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3

A

T

B

S

B

D

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,

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M

C

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K

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N

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ND4 FILTER

N D

S.

A.

8

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D

(7)

A

B

C

Figure 1.3-16

1-39

Chapter 1 System Configurations and Part Names

r

1.4 Name of Each Part

1.4.1 TIRF Illuminator Unit and Ti-U System

When used with two-laser or three laser-unit

TIRF illuminator unit

CLASS 3B –

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

CAUTION

To laser unit

Laser safety cove

To PC

TV camera (video camera)

TI-LUSU

shutter unit

Filter cube port cover retaining

RS-232C LASER UNIT

LASER SHUTTER

Exclusive use for TI-TIRF system

Don't use this unit with other systems.

INPUT 12V

SAFETY

1A

COVER CLOSE

BINO CLOSE

POWER

TI-LUSU

plate (part of laser safety kit)

When used with four-laser module A

SER RADIATIO OID EXPOSU CLASS 3B LASE

AVOID EXPOSURE

LASER LIGHT IS EMITTE

OBJECTIVE APERT

FROM

Figure 1.4-1

Filter slider cover (part of laser safety kit)

TI-FLC epi-fl filter turret

LASERSTRAHLUNG

CLASS 3B

–

KLASSE 3B,

WENN ABDECKUNG GEÖFFNET

NICHT DEM STRAHL AUSSETZEN

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

VORSICHT

CAUTION

HG fiber light source

Power supply device for the diascopic illumination

Optical path selector knob

To laser unit

Filter slider cover

(part of laser safety kit)

To laser unit

To PC

TV camera (video camera)

TI-LU4SU shutter unit LU4

Filter cube port cover retaining

plate

(part of laser safety plate)

TI-FLC epi-fl filter turret

TI-LU4SU

TIRF illuminator unit

Power supply device for the

CLASS 3B –

CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

LASERSTRAHLUNG

CLASS 3B

–

KLASSE 3B,

WENN ABDECKUNG GEÖFFNET

NICHT DEM STRAHL AUSSETZEN

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

VORSICHT

CAUTION

SER RADIATIO OID EXPOSU CLASS 3B LASE

AVOID EXPOSURE

LASER LIGHT IS EMITTE FROM

OBJECTIVE APERT

LASER EMISSION

POWER

diascopic illumination

HG fiber light source

Laser safety cover

Optical path selector knob

Figure 1.4-2

1-40

TI-TIRF illuminator unit

r

(7) Condenser lens locking screw

(3) Laser position adjustment knob

(for forward and backward adjustment)

(1) Optical fibe

(2) Laser position adjustment knob

(for right and left adjustment)

(8) Field diaphragm centering screws

Chapter 1 System Configurations and Part Names

1.4 Name of Each Part

(6) Focal point adjustment knob

(4) ND filter sliders

N D 2

N

D

8

16

VORSICHT

KLASSE 3B,

WENN ABDECKUNG GEFFNET

NICHT DEM STRAHL AUSSETZEN

CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

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L

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N D 8

(14) Connector for the

HG fiber light source adapter

(13) Excitation filter slider

(10) Aperture diaphragm

centering screws

(11) Aperture diaphragm

open/close lever

(9) Field diaphragm open/close lever

Parts used in the TIRF microscopy operation

(1) Optical fiber

(2) Laser position adjustment knob

(for right and left adjustment)

(3) Laser position adjustment knob

(for forward and backward adjustment)

(4) ND filter sliders

(5) Mirror switching lever

Push in: TIRF (60%) and Epi (40%) Pull out: TIRF (100%)

(6) Focal point adjustment knob

(7) Condenser lens locking screw

(5) Mirror switching lever

Figure 1.4-3

Parts used in the epi-fl microscopy operation

(8) Field diaphragm centering screws

(9) Field diaphragm open/close lever

(10) Aperture diaphragm centering screws

(11) Aperture diaphragm open/close lever

(12) ND filter sliders

(13) Excitation filter slider

(14) Connector for the HG fiber light source

Push in: TIRF (60%) and Epi (40%) Pull out: TIRF (100%)

adapter

1-41

Laser safety cover

C

A U T

I O N –

C L A

S S

3

L

B

A S E

R R A

D I A T I

O N

W

A

V

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P

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A

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Chapter 1 System Configurations and Part Names

1.4 Name of Each Part

The laser safety cover prevents laser light from leaking. If the laser safety cover is not attached, the motorized shutters of the laser unit do not open.

Laser safety cover

BEAM

DUCT

E TO

PRO

TION

UR

E

R

DIA

U

D

T

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R

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E S

AS

I

V I

L

T

AVOID EXPOS

T

CLA

C

H

E

G

EXPO

J

I B L

O R E

M S

VOID

O A

A

R L F

Safety interlock (both sides)

Figure 1.4-5

Upper cover

Body

C A U T

I O N

– C

L A S

S

3 L A

B S E

R R

A D IA

T I O

N

W A V

H

O

E I D

N

E

O

X

P

E O S

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E

A

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C H T

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R

L A

S

T

S

R

E

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3

L B

U ,

N

G

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N

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G C H

T

G

D

E

Ö

M

F F

S

N

T

E R A H

L A U

S S E

T

Z

E

Laser safety kit

C

A U T

I

O

N

–

L

A S E

R

A

V O I

D

E

X

V

O R S

I

C

H

T

K

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3

W

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N

A

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D

E

C A

U T

I O N

– C

L A S

S

L

3

A

B S E

R R

A D I A

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N

W

A V

H

O

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N

E

O

X

P

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U R

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T H E

B

E

A

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S I

C H T

– L

A S E

K

R

L A

S

T

S

R

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A H

3

L B

U ,

N

G

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N

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D E C

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N

I

G C H

T

G

D

E

Ö

M

F F

S

N

T

E R A H

L A U

S S E

T

Z

E

Protection plate

(for nosepiece)

N M

T N

Figure 1.4-4

C L A

S S

3

B D I A T I

O N

W

H

E

N

O P

P

E

O

N S U

R E

T O T H

E B

E

A

M – L A

S E R

S T R A

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B

U

,

N

G D E C K

U N G

G E

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M

F

F S

N T

E R

T

A H L

A U S

S E T

Z

E

N

A R R E S O V LA A

N M

T N

Figure 1.4-6

A V

O ID

E

X P O

S

U

LASER

R

E

LIGHT

IS

FROM

EMITTED

THIS

C

A

APERTURE

U T I O

N –

C L

A S S

3

L

B A S E R

R A D

IA T

I O N

W

A

V

H O

E

I

N

D

O

E

P

X

E

P

N O S U

R E

T O

T H

E B E

A

M

V

O R S I C

H T

– L A

S E

K

R

L

S

A

T

S

R

S

A

E

H

3

L U

B

N

,

G

W

E N N

A B D

E C K

U N

N

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G

T

E

D

Ö

E

F

M

F

S

N

T

E

R

T A H

L A

U S

S E T Z

E

N

Filter slider cover

AM

CT

U

BE

D

O

O R

T

N

E

P

IO

R

T

E

SU

IA

E

S

R

D O

U

D

P

LA

T

E

X

R

T

E

T

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I

E

3B

P

R

M

S

A

ID

U

E

S

E S

A

V

I

O

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P

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C

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J I B L O

ID

R

O

E

M

S

V

O

A

R

L F

A V

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E

X

P

O

S

U

LASER

R

E

LIGHT

IS

FROM

EMITTED

THIS

C

A

APERTURE

U T I O

N –

C L

A S S

3

L

B A S E R

R A D

I A T

I O N

W

A

V

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E

I

N

D

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P

X

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P

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A

M

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K

R

L

S

A

T

S

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S

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E

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3

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W

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N

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D

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E

F

M

F

S

N

T

E

R

T A H

L A

U S

S E T Z

E

N

ON

OFF

6V30W

12V100W

MAX.

ON

OFF

6V30W

12V100W

MAX.

Filter cube port cover retaining plate

* If the safety interlock part is touched or gotten wet,

misoperation may occur.

The laser safety kit is composed of the filter slider cover, protection plate, filter cube port fixed plate, and safety labels.

Before using the product, check that the part shown in the left figure is attached.

* For the types and positions of safety labels, see

Section 1.3.1, “Ti-U Microscope Body.”

1-42

TI-FLC epi-fl filter turret

A

r

Chapter 1 System Configurations and Part Names

1.4 Name of Each Part

Filter cube port (and its cover)

Front

Filter cube address and name on the optical path

TI-LUSU shutter unit

Safety cover signal connector

Laser unit connecto

Serial IF connecto

RS-232C LASER UNIT

Laser shutter

switch / indicato

Exclusive use for TI-TIRF system

Don't use this unit with other systems.

Figure 1.4-7

LASER SHUTTER

Figure 1.4-8

TI-FLC epi-fl filter turret

Shutter lever (O: open, C: close)

Filter cube rotation control

Eyepiece optical path

TI-LUSU

INPUT

12V

1A

signal connector

C adapter

connector

Safety cover close lamp

Eyepiece optical path closed lamp

Power switch

SAFETY COVER CLOSE

BINO CLOSE

POWER

Power lamp

The epi-fl filter turret is used to select an excitation method by rotating the turret to change filter cubes including a dichroic mirror.

The shutter unit controls motorized shutters in the laser unit.

Use this unit in combination with the C-LU2, C-LU3 or C-LU3EX laser unit.

TI-LU4SU shutter unit LU4

Laser unit connecto

Option switch

Laser shutter

switch

TI-LU4SU

Figure 1.4-9

Indicator lamp

LASER EMISSION

POWER

Power lamp

The shutter unit controls motorized shutters in the laser unit.

Use this unit in combination with the LU4A laser unit.

If the safety interlock functions, all the shutters are closed, and the lamp turns off.

To unlock the safety interlock, solve what caused the safety interlock being locked, then cancel the safety interlock from the software.

1-43

Chapter 1 System Configurations and Part Names

1.4 Name of Each Part

Optical path selector knob (on the microscope body)

The laser shutter does not open when the eyepiece route of the optical path (“EYE”) is selected.

PORT

Ti-U *

PORT dial (optical path selector knob)

Figure 1.4-10

EYE Observation port 100% R Right port 100% AUX Auxiliary port

(Observation/left port = 20:80) (Observation/right port = 20:80)

L Left port 100%

Ti-U/B

EYE Observation port 100% R Right port 100% B Bottom port 100% L Left port 100%

* The Ti-U is also available with an optical-path

splitter prism preinstalled at the auxiliary port. The light intensity ratio of visual observation to left port is 20 to 80. In this case, set the optical path selector knob as follows:

EYE Observation port 100% R Right port 100% L80 Visual observation/left port = 20:80 L Left port 100%

1-44

Chapter 1 System Configurations and Part Names

r

(

)

1.4 Name of Each Part

1.4.2 Motorized TIRF Illuminator Unit and Ti-E System

When used in combination with two-laser or three-laser unit

Motorized TIRF illuminator unit

CLASS 3B CLASS 3B – –

CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

CAUTION

LASER RADIATION WHEN OPEN

To laser unit

Laser safety cove

To PC

TV camera (video camera)

TI-LUSU

shutter unit

Filter cube port cover retaining

.

plate

part of laser safety kit)

Optical path selector switch

(front panel)

RS-232C LASER UNIT

LASER SHUTTER

Exclusive use for TI-TIRF system

Don't use this unit with other systems.

AVOID EXPOSURE TO THE BEAM

LASERSTRAHLUNG

LASERSTRAHLUNG CLASS 3B CLASS 3B

–

– – –

CAUTION

KLASSE 3B,

WENN ABDECKUNG GEÖFFNET

NICHT DEM STRAHL AUSSETZEN

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

VORSICHT CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

VORSICHT

KLASSE 3B,

WENN ABDECKUNG GEÖFFNET

NICHT DEM STRAHL AUSSETZEN

SER RADIATIO

OID EXPOSU

CLASS 3B LASE

AVOID EXPOSURE

LASER LIGHT IS EMITTE

FROM OBJECTIVE APERT

FROM

OBJECTIVE APERT

INPUT 12V

SAFETY

1A

COVER CLOSE

BINO CLOSE

POWER

TI-LUSU

Figure 1.4-11

Filter slider cover (part of laser safety kit)

TI-FLC-E or TI-FLC-E/HQ motorized epi-fl filter turret, with Protection plate (part of laser safety kit)

HG fiber light source

Power supply device for the diascopic illumination

TI-RCP remote control pad

When used with four-laser module A

To laser unit

CLASS 3B CLASS 3B – –

CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM CAUTION

LASER RADIATION WHEN OPEN

TI-LU4SU

AVOID EXPOSURE TO THE BEAM

LASER EMISSION

POWER

To laser unit

To PC

To laser unit

To PC

TV camera (video camera)

TI-LU4SU shutter unit LU4

Filter cube port cover

retaining plate

TI-FLC-E/HQ motor epi-fl

filter turret,

with protection plate

(part of laser safety kit)

Figure 1.4-12

Motorized TIRF illuminator

Hub controller A

with AC adapter

Power supply device for the diascopic illumination

HG fiber light source

Remote control pad

1-45

Chapter 1 System Configurations and Part Names

r

1.4 Name of Each Part

TI-TIRF-E motorized illuminator unit and remote control pad

(5) Condenser lens locking screw

(2) Laser position adjustment knob

(for forward and backward adjustment)

(4) Focal point adjustment knob

(3) ND filter sliders

CAUT

L

A

V

VORSICH

S KL WEN

OID E

NICHT DEM

ER

I

A

O

S

R

N

S

N

–

AD

X

E

PO

A

3B,

C

T

B

IA

LAS

–

D

S

T

E

LASE

URE T

IO

S

C

S

TRAHL

N W

KUNG

3B

R

O T

HE

STR

N O

AUS

HE

G

AHLUNG

E

P

B

Ö

SET

E

FFNET

N

AM

Z

EN

M

G

NET A PEN

BE O N

THE

E B

TO

WH 3

ERSTRAHLUN

RE

U TION CLASS

POS – N

RADIA O I

OID EX UT A

LASER C

(10) ND filter sliders

AUSSETZEN

AHL R

KUNG GEÖFF

T

S LAS –

DEC

M

3B,

HT

AB

DE

E

C

T

SI

ICH N

WENN

KLASS

VOR

AV

(12) Connector for the

HG fiber light source adapter

(1) Optical fibe

CAUTION

LASER R

–

AVO

CLASS 3B

A DIA

ID EXPOSURE

TION WHEN OPE

V O RSIC

TO T

WE

H

N

T

NN

HE BEAM

I

N

CHT

– LA

AB

D

S

DECK

EM ST

E RS

UNG

T

R

RAHLU

AHL

G

A

EÖFFN

USSETZEN

NG

E T

(11) Excitation filter slider

(8) Aperture diaphragm

centering screws

(9) Aperture diaphragm

(6) Field diaphragm centering screws

open/close lever

(7) Field diaphragm open/close lever

Figure 1.4-13

Parts used in the TIRF microscopy operation

(1) Optical fiber

(2) Laser position adjustment knob

(for forward and backward adjustment *)

(3) ND filter sliders

(4) Focal point adjustment knob

(5) Condenser lens locking screw

Parts used in the epi-fl microscopy operation

(6) Field diaphragm centering screws

(7) Field diaphragm open/close lever

(8) Aperture diaphragm centering screws

(9) Aperture diaphragm open/close lever

(10) ND filter sliders

(11) Excitation filter slider

(12) Connector for the HG fiber light source

adapter

The TI-RCP remote control pad (or TI-ERGC ergo controller) allows users to change illumination to TIRF or episcopic as well as adjust the laser right or left (TIRF illumination angle adjustment).

TI-RCP remote control pad

TIRF Angle button

Figure 1.4-14

TIRF / EPI button

User can perform the following for the TI-TIRF-E the remote control pad:

• TIRF/EPI button:

The illumination is changed to TIRF (laser light) or episcopic.

• TIRF Angle button:

TIRF illumination position (illumination angle) adjustment *

* The laser is adjusted right or left and the

illumination angle of TIRF illumination (total reflection illumination) is adjusted.

1-46

Laser safety cover

C A U

T I O

N – C L

A S S 3

L

B A S

E R R

A D I A

T I O

N

W A V

H

O

E

I

N

D

O

E

P

X

E

P

N O S

U R

E T

O T

H E

B

E

A

M V O R

S I C

H T – L

A S

E

K

R

L

S

A

T

S

R

S

A

E

H

3

L

B

U

,

N

G W E N

N A

B D E C

K U

N

G I C

H T

G

E

D

Ö

E

F

M

F

S

N

T

E

R

T

A

H

L

A U

S S E

T Z E

N

Chapter 1 System Configurations and Part Names

1.4 Name of Each Part

The laser safety cover prevents laser light from leaking. If the laser safety cover is not attached, the motorized shutters of the laser unit do not open.

Laser safety cover

M T

A

C

E

U

B

D

O

O T R

N

P

E O I R

R

T

U

E

A

S

I

E

S

R

D

O

A

U

D

L

A

P

T

E

X

R

T

B

E

T

E

3

I

E

R

P

R

M

E

S

A

ID

U

E

S

E

O

S

A

V

I

V

O

I

L

T

A

P

T

C

H

X

E

G

E

J

I

B

L

D

O

I

R

O

E

M

S

V

O

A

R

L

F

Safety interlock (both sides)

Figure 1.4-16

Upper cover

Body

C A U T

I O N

L

A S E

R R A

A

V O I

D E X

V

O R S

I C H T

K

L A S S

E 3 B

W

E N N

A B

D N I

C H T

D

E

M

Laser safety kit

C A U T

I

O

N L

A S E

R

A A

V O I

D

E

X

V

O R S

I

C

H

T K

L A S S

E

3

B W

E N N

A

B

D N I

C H T

D

E

M

Protection plate

(for nosepiece)

– C L A

S S

3

B D I A T I

O N

W

H

E

N O P

P

E

O

N S U

R E

T O T H

E B

E

A

M – L A S

E R

S T R A

H L U

,

N

G E C K U

N G

G E

Ö F F

S

N T

E R

T A

H L

A U S S

E T Z

E

N

Figure 1.4-15

C A U

T I O

N

–

C

L

A S

S 3

L

B A S

E R R

A D I A

T I O

N

W A V

H

O

E

I

N

D

O

E

P

X

E

P

N O S

U R

E T

O T

H E

B

E

A

M V O R

S I C

H T – L

A S

E

K

R

L

S

A

T

S

R

S

A

E

H

3

L

B

U

,

N

G W E N

N A

B D E C

K U

N

G I C

H T

G

E

D

Ö

E

F

M

F

S

N

T

E

R

T

A

H

L

A U

S S E

T Z E

N

A E B O T R

N

P

E O I

R

T

U

E

A

S

I

S

D

O

A

L

A

P X

R

B

E

R

3

E

S

ID

S

O

A

V

L

A

C

– C L A

S S

3

B D I A T I

O N

W

H

E

N O P

P

E

O

N S U

R E

T O T H

E B

E

A

M – L A S

E R

S T R A

H L U

,

N

G E C K U

N G

G E

Ö F F

S

N T

E R

T A

H L

A U S S

E T Z

E

N

Figure 1.4-17

A V

O

I D

E

X

P O

S

L

U

A

R

S

E

R

L I

G H

T

I S

F

E

R

M

O

I

M

T

T E

T

D

H

I S

C

A U

A

T

P

I O

E

N

R

–

T

C

U

L

R

A S

E

S 3

L

B A S

E R R

A D I A

T I O

N W

A

H V O

E

N I D

O E

P

X

E

P

N O S

U R

E T

O T

H E

B

E

A

M

V

O R

S I C H

T –

L A S

E

K

R

L

S

A

T

S

R

S

A

E

H L

3

U B

N ,

G

W

E N

N A B

D E C

K U

N

G I C H

G

T

E

D

Ö

E

F

M

F N

S

E

T R

T

A

H

L

A U

S S E

T Z E

N

Filter slider cover

M T C U D O

E R U

D

T

E

R

T

E

T

E I P

R

M

A

U

E

S

E V

IS

O

I T

P

T

C

H

X

E

J

IG

B

L

D

O

I

R

O

E

M

S

V

O

A

R

L

F

A V

O

I D

E

X

P O

S

L

U

A

R

S

E

R

L I

G H

T

I

S

F

E

R

M

O

I

M

T

T E

T

D

H

I S

C

A U

A

T

P

I O

E

N

R

–

T

C

U

L

R

A S

E

S 3

L

B A S

E R R

A D I A

T I O

N W

A

H V O

E

N I D

O E

P

X

E

P

N O S

U R

E T

O T

H E

B

E

A

M

V

O R

S I C H

T –

L A S

E

K

R

L

S

A

T

S

R

S

A

E

H L

3

U B

N ,

G

W

E N

N A B

D E C

K U

N

G I C H

G

T

E

D

Ö

E

F

M

F N

S

E

T R

T

A

H

L

A U

S S E

T Z E

N

RESET

-

Coarse Fine ExFine

ON/OFF

6V 30W

Coarse Fine ExFine

ON/OFF

6V30W

Z

DISPLAY

RECALL

BRIGHTNESS

Obj.

MEMORY

FOCUS

PFS

ON

EYE

R100

L100

12V100W

L80

MAX.

Obj.

12V100W

MAX.

RESET

Z

DISPLAY

RECALL

BRIGHTNESS

MEMORY

FOCUS

PFS

ON

EYE

R100

L100

L80

Filter cube port cover retaining plate

* If the safety interlock part is touched or gotten wet,

misoperation may occur.

The laser safety kit is composed of the filter slider cover, protection plate, filter cube port fixed plate, and safety labels.

Before using the product, check that the part shown in the left figure is attached.

* For the types and positions of safety labels, see

Section 1.3.2, “Ti-E Microscope Body.”

1-47

Chapter 1 System Configurations and Part Names

r

A

r

1.4 Name of Each Part

TI-FLC-E motorized epi-fl filter turret or TI-FLC-E/HQ motor epi-fl filter turret

Filter cube port (and its cover)

Front

TI-LUSU shutter unit

Safety cover signal connecto

Laser unit connecto

Serial IF connecto

RS-232C LASER UNIT

Laser shutter

switch / indicato

Exclusive use for TI-TIRF system

Don't use this unit with other systems.

Shutter lever (O: open, C: close)

Figure 1.4-18

LASER SHUTTER

Figure 1.4-19

TI-FLC-E motorized epi-fl filter turret or TI-FLC-E/HQ motorized epi-fl filter turret

Eyepiece optical path signal connector

C adapter

connector

Safety cover

INPUT

1A

close lamp

Eyepiece optical path closed lamp

Power switch

SAFETY COVER CLOSE

BINO CLOSE

POWER

12V

TI-LUSU

Power lamp

The epi-fl filter turret is used to select an excitation method by rotating the turret to change filter cubes including a dichroic mirror.

The shutter unit controls motorized shutters in the laser unit.

Use this unit in combination with the C-LU2, C-LU3 or C-LU3EX laser unit.

TI-LU4SU shutter unit LU4

Laser unit connecto

Option switch

Laser shutter

switch

TI-LU4SU

Figure 1.4-20

Indicator lamp

LASER EMISSION

POWER

Power lamp

The shutter unit controls motorized shutters in the laser unit.

Use this unit in combination with the LU4A laser unit.

If the safety interlock functions, all the shutters are closed, and the lamp turns off.

To unlock the safety interlock, solve what caused the safety interlock being locked, then cancel the safety interlock from the software.

1-48

Chapter 1 System Configurations and Part Names

(

)

1.4 Name of Each Part

Optical path selector switch (on the microscope body)

The laser shutter does not open when the nosepiece tube route of the optical path (“EYE”) is selected.

Coarse

Fine

BRIGHTNESS

FOCUS

DISPLAY

-

Z

RESET

EYE

ON

MEMORY

L100

PFS

R100

L80

1.5X

1X

Epi Shutter

ExFine

FL Block

Refocus

Escape

Optical path selector switch

Figure 1.4-21

Ti-E

EYE Observation port 100% L100 Left port 100% L80 Left port 80%

and observation port 20%

R100 Right port 100%

Ti-E/B

EYE Observation port 100% L100 Left port 100% L80 Left port 80%

and observation port 20%

B100 Bottom port 100%

1.4.3 Motorized TIRF Illuminator Unit, LU4A, N-STORM and Ti-E System

When used with four-laser module A

Motorized TIRF illuminator unit

Hub controller A

with AC adapter

Power supply device for the diascopic illumination

HG fiber light source

To laser unit

STORM slider

CLASS 3B CLASS 3B – –

CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

CAUTION

To PC

To laser unit

To PC

EM-CCD camera

TI-LU4SU shutter unit LU4

3D-STORM port

Filter cube cover retaining

plate

TI-LU4SU

LASER EMISSION

POWER

Remote control pad

Figure 1.4-22

TI-FLC-E/HQ motor epi-fl filter turret, with protection plate (part of laser safety kit)

1-49

Chapter 1 System Configurations and Part Names

r

TI-TIRF-E motorized illuminator unit

(6) STORM slider

(2) Laser position adjustment knob

(for forward and backward adjustment)

1.4 Name of Each Part

(5) Condenser lens locking screw

(4) Focal point adjustment knob

(3) ND filter sliders

CAUT

LA

AV

V

SER

KLASSE 3

W

O

NICHT DEM

I

ID

RSICH

E

O

NN

N

RADI

E

–

X

PO

ABDECKUNG

C

T

A

LASS

B

–

SUR

TI

,

LA

O

STR

N W

SE

E

3B

A

T

RSTR

HL

O

HEN OPEN

T

AUS

HE BEAM

G

AHLU E Ö

SETZEN

FFNET

NG

(11) ND filter sliders

N

G

NET

AM

N

TZE E

OPEN

HE BE

GEÖFF

T

AUSS O T

NG

AHL

E

R

T

LASERSTRAHLU

TION WHEN

–

DECKU

M S

B, CLASS 3B

T

–

ADIA

H

AB

DE

E 3

N

R

N

HT

ID EXPOSU

ASS

L

NIC

WEN

K

VORSIC

AVO

LASER

CAUTIO

(13) Connector for the

HG fiber light source adapter

(1) Optical fibe

CAUTION

LASER RADIA

–

AVO

CLASS 3B

ID EXPOSURE

TION WHEN OPE

VO

RS

I

TO T

C

WE

HT

N

HE BEAM

ICHT DE

N

– L

A

BDE

SERST

M

C K

S

UN

T RAH

RAHL

G

L

G

AUSSET

UNG

EÖFF

NET

Z

E N

(12) Excitation filter slider

(9) Aperture diaphragm

centering screws

(7) Field diaphragm centering screws

(10) Aperture diaphragm

open/close lever

(8) Field diaphragm open/close lever

Figure 1.4-23

Parts used in the TIRF microscopy operation

(1) Optical fiber

(2) Laser position adjustment knob

(for forward and backward adjustment)

(3) ND filter sliders

(For N-STORM, replace the ND16 by lambda plate slider.)

(4) Focal point adjustment knob

(5) Condenser lens locking screw

(6) STORM slider

Parts used in the epi-fl microscopy operation

(7) Field diaphragm centering screws

(8) Field diaphragm open/close lever

(9) Aperture diaphragm centering screws

(10) Aperture diaphragm open/close lever

(11) ND filter sliders

(12) Excitation filter slider

(13) Connector for the HG fiber light source

adapter

The TI-RCP remote control pad (or TI-ERGC ergo controller) allows users to change the illumination to TIRF or episcopic as well as adjust the laser right or left (TIRF illumination angle adjustment).

TI-RCP remote control pad

TIRF Angle button

TIRF / EPI button

Figure 1.4-24

User can perform the following for the TI-TIRF-E the remote control pad:

• TIRF/EPI button:

The illumination is changed to TIRF (laser light) or episcopic.

• TIRF Angle button:

TIRF illumination position (illumination angle) adjustment *

* The laser is adjusted right or left and the

illumination angle of TIRF illumination (total reflection illumination) is adjusted.

1-50

Laser safety cover

C A

U T I

O N

– C

L A S

S L A S

E R

R A

D I

A

T

I

O

N A V O

I D

E X

P O

S

U

R

E V O R

S I C

H T

–

L

A

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K

R

L A

S S E

3 B

,

W

E N

N

A B D

E C

K

U

N

N I

G C H

T D

E M

S T

R

A

H

L

Chapter 1 System Configurations and Part Names

C A U T

I O N

– C

L A S S

3

L

B

A S E

R R

A D I

A T I O N

W A V

H O

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P

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Figure 1.4-25

AVOID

EXPOSURE

L A

S E

R

L I

G H

T I

S

F

E

R

M

O

IT

M

T E

T

D

H

I S

C

A

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P

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N

R

–

T

C

U R

E

L

A S E

R R

A D I

A

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P O

V

O R S

I C H

T –

K

L A S S

E

3 B ,

W

E N N

A

B D E

N

I C H T

D E

M S

Laser safety cover

L A S

S

3

B A T I O

N W

H

E N O

P E

N S U R

E T

O T

H E

B

E

A

M L A S

E R

S T R

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N G

G

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F

N T

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T

A H L

A U

S S E

T

Z

E

N

Coarse Fine ExFine

Obj.

ON/OFF

6V30W

12V100W

MAX.

1.4 Name of Each Part

The cover prevents laser light from leaking. If the laser safety cover is not attached, the motorized shutters of the laser unit do not open.

Safety interlock (both sides)

RESET

Z

DISPLAY

RECALL

BRIGHTNESS

MEMORY

FOCUS

PFS

ON

EYE

R100

L100

L80

* If the safety interlock part is touched or gotten

wet, misoperation may occur.

When using piezo-stages:

Figure 1.4-26

Upper cover

Body

TI-S-ER Motorized Stage with Encoders/ TI-S-E Motorized Stage

Piezo Z stage

Figure 1.4-27

Laser safety cover

TI-TIRF-LSC Laser Safety Cover/PZT

To use the piezo-stage together, the TI-TIRF-LSC Laser safety cover/PZT is necessary. For how to attach the TI-TIRF-LSC Laser safety cover/PZT, see “Setup procedure and cautions” supplied with the TI-TIRF-LSC Laser safety cover/PZT. Even when the TI-TIRF-LSC Laser safety cover/PZT is attached, the motorized shutters of the laser unit do not open unless the laser safety cover is attached.

* If the safety interlock part is touched or gotten

wet, misoperation may occur.

1-51

Laser safety kit

r

Chapter 1 System Configurations and Part Names

1.4 Name of Each Part

Filter slider cover

The laser safety kit is composed of the filter slider cover, protection plate, filter cube port cover retraining plate, and safety labels.

C A U

T I O

N – C

L A S

S 3

L

B

A S

E R

R A D I A

T I O

N

W A V

H

O

E

I

N

D

O E X

P

E

O

N

S

U R

E T

O T

H

E

B E A

M V O R

S I C

H T –

L A S

E

K

R L A

S

T R

S E

A H

3

L

B

U

,

N

G W E

N N

A B D E

C K U

N

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C

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G

D

E Ö

E M

F F

S

N

T

E

R

T

A

H L

A U

S S E

T Z

E

N

Before using the product, check that the part shown in the left figure is attached.

* For the types and positions of safety labels, see

Section 1.3.2, “Ti-E Microscope Body.”

Protection plate

(for nosepiece)

C A U

T I O N

– C L

A S S

L

3

A

B

S

E R R

A D I A T

I O N

A

W

V

H

O

E

I D

N

E

O

X

P

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T O T

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A

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V

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I C H T

– L A

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K

R

L A

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T

S

R

E

A

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3

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U

,

N

G

W

E N N

A B

D E C K

U

N

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G

D

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M

F F

S

N

T

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T A H L

A U S

S E T

Z

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N

Figure 1.4-28

M T

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V

O

A

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AVOID

EXPOSURE

L A

S E

R

L I

G

H

T

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F

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M

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M

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B A S E

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A

M

V

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S I C H T

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K

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A

T

S

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S

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T

A

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N

Coarse Fine ExFine

ON/OFF

6V30W

RESET

Z

DISPLAY

RECALL

BRIGHTNESS

Obj.

MEMORY

FOCUS

PFS

ON

EYE

R100

L100

12V100W

L80

MAX.

Filter cube port cover retaining plate

TI-FLC-E motorized epi-fl filter turret or TI-FLC-E/HQ motor epi-fl filter turret

Filter cube port

(and its cover)

TI-FLC-E or TI-FLC-E/HQ

The epi-fl filter turret is used to select an excitation method by rotating the turret to change filter cubes including a dichroic mirror.

Front

Figure 1.4-29

TI-LU4SU shutter unit LU4

Laser unit connecto

Option switch

Laser shutter

switch

TI-LU4SU

Figure 1.4-30

Shutter lever (O: open, C: close)

Indicator lamp

LASER EMISSION

POWER

Power lamp

The shutter unit controls motorized shutters in the laser unit.

Use this unit in combination with the LU4A laser unit.

If the safety interlock functions, all the shutters are closed, and the lamp turns off.

To unlock the safety interlock, solve what caused the safety interlock being locked, then cancel the safety interlock from the software.

1-52

Chapter 1 System Configurations and Part Names

r

Optical path selector switch

Coarse

Fine

BRIGHTNESS

FOCUS

DISPLAY

Z RESET

EYE

ON

MEMORY

L100

PFS

R100

L80

1.5X

1X

ExFine

Epi Shutter

FL Block

Refocus

Escape

Optical path selector switch

Figure 1.4-31

N-STORM

1.4 Name of Each Part

The laser shutter does not open when the nosepiece tube route of the optical path (“EYE”) is selected.

Ti-E

EYE Observation port 100% L100 Left port 100% L80 Left port 80% and observation port 20% R100 Right port 100%

Ti-E/B

EYE Observation port 100% L100 Left port 100% L80 Left port 80% and observation port 20% B100 Bottom port 100%

Lambda plate

slide

STORM slide

DSC support column

3D-STORM port

The 3D-STORM port connecting the Ti-E microscope and EM-CCD camera when performing the N-STORM microscopy is equipped with the cylindrical lens that is placed in or out of the optical imaging system to the camera by sliding the handle. In normal microscopy and 2D-STORM microscopy, remove the cylindrical lens out of the optical path, and in capturing the 3D-STORM

Ti-E fixing plate

image, insert it in the optical path.

If the cylindrical lens is installed during normal microscopy and 2D-STORM microscopy, images

Cylindrical lens IN/OUT handle

OUT

IN

are distorted. In 3D-STORM microscopy, the shape of each bright spot changes to oval.

(When using this port together with the confocal microscope A1, install the 3D-STORM port in the right port. When using this port independently, install it in the left port.)

3D-STORM port

Combination of A1 and 3D-STORM port

Figure 1.4-32

1-53

Chapter 1 System Configurations and Part Names

1.4 Name of Each Part

1.4.4 TI-PAU Photo Activation Illuminator Unit and Ti-U System

When used in combination with two-laser or three-laser unit

Specimen cover

TI-FLC epi-fl filter turret

Photo activation illuminator unit

Interlock cable

TI-LUSU shutter unit

CLASS 3B –

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

CAUTION

Figure 1.4-33

When used with four-laser module A

Interlock cable

Photo activation

illuminator unit

TI-LU4SU shutter

unit LU4

Specimen cover

TI-FLC epi-fl filter turret

CLASS 3B –

CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

Figure 1.4-34

1-54

Chapter 1 System Configurations and Part Names

r

A

Photo Activation Illuminator Unit

1.4 Name of Each Part

Laser focus adjustment knob

Optical fiber

Laser position adjustment knob (for right and left adjustment)

ND filter sliders

Mirror switching lever

• Push in: Mirror IN (epi illumination)

• Pull out: Mirror OUT (laser light)

(* with the total reflection mirror)

Specimen cover

Laser position adjustment knob (for forward and backward adjustment)

Laser focus adjustment clamp screw

CAUTION

LASER RADIATION WHEN OPEN

AVOID EXPOSURE TO THE BEAM

VORSICHT

KLASSE 3B,

WENN ABDECKUNG GEFFNET

NICHT DEM STRAHL AUSSETZEN

  LASERSTRAHLUNG

IN

ND2

N

D

16

8

2

perture diaphragm

open/close lever

Field diaphragm centering screws

Field diaphragm open/close lever

Figure 1.4-35

HG fiber light source

uxiliary filter slider

adapter/lamphouse mounting part for epi illumination

TZEN

LASERSTRAHLUNG

 CLASS 3B 

NICHT DEM STRAHL AUSSE

WENN ABDECKUNG GEFFNET

KLASSE 3B,

VORSICHT

AVOID EXPOSURE TO THE BEAM

LASER RADIATION WHEN OPEN

CAUTION

CLASS 3B

S.

A.

CAUTION

LASER RADIATION WHEN OPEN



AVOID EXPOSURE TO THE BEAM

CLASS 3B

VORS

K

I

L

CHT  LASERSTRAHLUNG

ASSE

WEN

N

I

N

CH

ABDECKUNG GE

3B

T D

,

EM

S TRAHL

A USSETZ

FFNET

EN

FILTER

ND8

ND4

N D 8

N D 4

Excitation filter slide

ND filter sliders

perture diaphragm

centering screws

CAUTION

–

C LASS

3B LAS ER

RAD

IATIO

N W A VO

HEN OP

ID

EX

EN

POS

URE TO THE B

EAM

VORSI

CHT

– LA

SERSTRAHLUNG KLASS

E 3

B, WENN ABDECKUNG G NICHT DEM STRAHL AUSSET

EÖF FNET ZEN

The cover prevents illumination light from leaking.

Specimen cover

M

T

T A

A

C

C E

E

U

U B

B

D

D O

O

T

R

N

N E

E

P

IO

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N

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T A H L A

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ON

OFF

6V30W

12V100W

MAX.

Figure 1.4-36

1-55

Laser safety kit

Chapter 1 System Configurations and Part Names

1.4 Name of Each Part

C

A U

T I O N

–

C L A

S S 3

L

B A S E

R R

A D

I A T

I O N

W

A

V

H

O

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I

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D

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P

X

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F

M

F

S

N

T

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T A H

L A

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S E T

Z E

N

Safety label (7)

Protection plate (for nosepiece)

M

M CT

CT ODU

ODU

N ION T

TIO

URE TO BEA

URE TO BEA IA

E

E D

D

ADIA

POS

LASER PR

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T

A M

–

L

K

A

L

S

A

E

S

R

S

V

E

O

T R

R

3

A

B

S

H

I

,

C

L

H

W

U

T

N

E

–

G

N N

L A

A

S

B

E

K

D

R

L

A

S

E

S

T

C

N

R

S

K

I

E

A

U

C

H

N

H

3

L

G

T

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U

,

D

N

E

G

M

E Ö

S

W

T

F

E

R

F

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A

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H

T

L

A B

A

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S

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N

Z

I

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N

H T

G

D

E Ö

E M

F F

S

N

T

E

R

T A H L

A U

S S

E T Z

E

N

Safety label (9) (both sides)

Safety label (7) (both sides)

Figure 1.4-37

TI-FLC epi-fl filter turret

Filter slider cover

A

V O ID

E

X P

O

S U

L AS

R

E

E R

L IG

H

T IS

F

E

R

M

O

IT

M

T E

T

D

H

IS

C A

A

U

T

PE

I O

N

RT

–

C

U

L

R

A S

E

S 3

L

B A S

E R

R A D

I A T

I O N W

A

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E

N I D

O E

P

X

E

P

N O S

U R E T

O T

H E

B

E

A

M V O R

S I C

H T

– L

A S E

K

R

L

S

A

T

S

R

S

A

E

H L

3

U

B

N

,

G W E

N N

A B D

E C

K U N

N

G I C

H

G

T

E

D

Ö

E

F

M

F N

S

E

T

R

T A H L

A U

S S E

T Z E

N

Filter cube port cover retaining plate

ON

OFF

6V30W

12V100W

MAX.

The laser safety kit is composed of the filter slider cover, protection plate for nosepiece, filter cube port cover retaining plate, and safety labels, “(9) IEC aperture label 2” and “(7) IEC caution label – Class 3B areas”. Before using the microscope, CHECK that each part of the laser safety kit is correctly attached. (See the figure left.)

(7) IEC caution label - Class 3B areas

CAUTION – CLASS 3B LASER RADIATION WHEN OPEN AVOID EXPOSURE TO THE BEAM

–

VORSICHT KLASSE 3B, WENN ABDECKUNG GEÖFFNET NICHT DEM STRAHL AUSSETZEN

(9) IEC aperture label 2

AVOID EXPOSURE

LASER LIGHT IS EMITTED FROM

LASERSTRAHLUNG

THIS APERTURE

Front

Filter cube port (and its cover)

Filter cube address and name on the optical path

Filter cube rotation control

Figure 1.4-38

TI-FLC epi-fl filter turret

Shutter lever (O: open, C: close)

The epi-fl filter turret is used to select an excitation method by rotating the turret to change filter cubes including a dichroic mirror.

1-56

TI-LUSU shutter unit

A

r

Chapter 1 System Configurations and Part Names

1.4 Name of Each Part

Safety cover signal connector

Laser unit connecto

Serial IF connecto

RS-232C LASER UNIT

LASER SHUTTER

Laser shutter

switch / indicato

Exclusive use for TI-TIRF system

Don't use this unit with other systems.

Figure 1.4-39

TI-LU4SU shutter unit LU4

Laser unit connecto

Option switch

TI-LU4SU

Laser shutter

switch

Figure 1.4-40

Eyepiece optical path

TI-LUSU

INPUT

12V

1A

signal connector

C adapter

connector

Safety cover close lamp

Eyepiece optical path closed lamp

Power switch

SAFETY COVER CLOSE

BINO CLOSE

POWER

The shutter unit controls motorized shutters in the laser unit.

Use this unit in combination with the C-LU2, C-LU3 or C-LU3EX laser unit.

Power lamp

Indicator lamp

The shutter unit controls motorized shutters in the laser unit.

Use this unit in combination with the LU4A laser

LASER EMISSION

POWER

unit.

If the safety interlock functions, all the shutters are closed, and the lamp turns off.

To unlock the safety interlock, solve what caused

Power lamp

the safety interlock being locked, then cancel the safety interlock from the software.

Optical path selector knob (on the microscope body)

The laser shutter does not open when the binocular route of the optical path (“EYE”) is selected.

PORT

PORT dial (optical path selector knob)

Ti-U *

EYE Visual observation 100% R Right port 100% AUX Auxiliary port

L Left port 100%

Figure 1.4-41

* The Ti-U is also available with an optical-path

splitter prism preinstalled at the auxiliary port. The light intensity ratio of visual observation to left port is 20 to 80. In this case, set the optical path selector knob as follows:

EYE Observation port 100% R Right port 100% L80 Visual observation/left port = 20:80

L Left port 100%

(Visual observation/left port = 20:80) (Visual observation/right port = 20:80)

Ti-U/B

EYE Visual observation 100%

R Right port 100% B Bottom port 100%

L Left port 100%

1-57

Chapter 1 System Configurations and Part Names

(5)

(6)

1.4 Name of Each Part

1.4.5 Laser Unit

Two-laser Unit (C-LU2)

(1) Laser mounting position L1

(2)

(1)

(3)

(4)

Figure 1.4-42

* For details of available lasers for each position of laser units, see Section 8.1.4, “Mountable Lasers” in

Chapter 8, “Specifications and Performance.”

(2) Laser mounting position L2

(3) Shutter unit connector

(4) ND filter slider

(5) Single-mode fiber

Three-laser unit (C-LU3)

(1) Laser mounting position L1

(2) Laser mounting position L2

(3) (1)

(3) Laser mounting position L3

(4) Shutter unit connector

(2)

(5) ND filter slider

(6) Single-mode fiber

(4)

(5)

Figure 1.4-43

* For details of available lasers for each position of laser units, see Section 8.1.4, “Mountable Lasers” in

Chapter 8, “Specifications and Performance.”

1-58

Chapter 1 System Configurations and Part Names

Three-laser unit EX (C-LU3EX)

(2)

(3)

(5)

(4)

1.4 Name of Each Part

(1) Laser mounting position L1

(2) Laser mounting position L2

(3) Laser mounting position L3

(4) Shutter unit connector

(1)

(5) Single-mode fiber

(6) Brightness control knob

(7) Ar wavelength selector (optional)

(8)

(8) AOM connector (optional)

(6)

(7)

Figure 1.4-44

(6)

* For details of available lasers for each position of laser units, see Section 8.1.4, “Mountable Lasers” in

Chapter 8, “Specifications and Performance.”

1-59

Chapter 1 System Configurations and Part Names

*

Four-laser module A (LU4A)

transportation.

Fix the laser unit with

(3)

(4)

(5)

(7)

(8)

four blocks during

Figure 1.4-45

(9) (14)

(11)

Figure 1.4-46

(10)

1.4 Name of Each Part

(1) (2)

1

(6)

(13)

(15) (12)

POWER

L3

L2

L4

L1

(1) Laser mounting position L1

(2) Laser mounting position L2

(3) Laser mounting position L3

(4) Laser mounting position L4

(5) Single-mode fiber

*1

(6)

ND filter sliders

(7) Power switch

(8) AC inlet

(9) Remote switch

(10) CONTROLLER connector

(Do not use in this system.)

(11) AOTF/PC connector

(Do not use in this system.)

(12) Interlock cable connectors

(13) LUSU connector

(14) USB connector

(15) LD connector

(Do not use in this system.)

*1: Installed only when the N-STORM kit is

mounted

Precautions when handling the laser unit

• The laser head and the optical fiber are precisely aligned in these units. Avoid subjecting the laser head or optical fiber to vibration or shock. Contact the distributor if you need to remove the laser head from the unit due to laser malfunction or other reasons.

Indicating shutter conditions

Figure 1.4-47

* For details of available lasers for each position of laser units, see Section 8.1.4, “Mountable Lasers” in

Chapter 8, “Specifications and Performance.”

1-60

Chapter 1 System Configurations and Part Names

Four-laser PS rack (LU-LR)

(1)

(Front view)

(5)

(4)

1.4 Name of Each Part

(1) EMISSION laser indicator

(2)

(2) POWER laser indicator

(3) EMISSION key switch

(3)

(4) Power switch

(5) INTERLOCK connector

(6) Cooling fan

(7) Power breaker for 15 A line

(8) Power breaker for 5 A line

(9) Caution label

(10) Name plate for 15 A line ratings

(11) Name plate for 5 A line ratings

(12) AC inlet for 15 A line

(6)

(13) AC inlet for 5 A line

(7)

(9) (10) (12)

(11)

(13)

(Rear view)

Figure 1.4-48

(8)

1-61

Chapter 1 System Configurations and Part Names

1.4 Name of Each Part

1.4.6 Laser Safety Kit

The laser safety kit is supplied with all laser products mounted onto a Ti microscope.

Labels affixed to each laser product include a name label and other types of necessary labels, both of which are not shown here.

The laser safety kit is composed of the filter slider cover, protection plate for nosepiece, filter cube port cover retaining plate, and safety labels, “(7) IEC caution label – Class 3B areas” and “(9) IEC aperture label”. Before using the microscope, CHECK that each part of the laser safety kit is correctly attached. (See the figure below.)

Safety label (7)

Protection plate

(for nosepiece)

Analyzer port

Safety label (9)

(Both sides)

Filter slider cover

C A U T

IO N

–

C L

A S S

3

LA

B S E

R R

A D

I A T

I O N

W A V

H

O

E

ID

N O

E

P

X

E

P

N

O

S U

R E

T

O

T H

E B E

A

M V O R S

I C H

T –

L A

S E

K

R

L

S

A

T

S

R

S

A

E

H

3

L

B

U

,

N

G W E N N

A

B D

E C

K U N

N

G

I C H

G

T

E

D

Ö E M

F

F S

N

T

E

R

T A H

L

A U S

S E T Z

E

N

A R R E

S

O

A

V

L

A

C

A U

T IO

N –

C L A S

S

L

3

A

B

S

E R

R A D

I A T IO

N W

A

V

H

O

E I D

N E

O

X

P

E

O

N S U R

E T O

T H E

B E

A

M

V O

R S

I C

H T –

L A S

E

K

R

L

A

S

T

S

R

E

A H

3

L

B

U

,

N

G

W E

N N

A

B D E

C K U

N

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G

C

H T

G

D

E

Ö

M

F F

S

N

T

E R

T A H

L A

U S S E

T Z

E

N

Figure 1.4-49

Filter slider cover

M

T

A

C

E

U

B

D

O

T

R

N

P

E O I

R

T

U

E

A

S

I

E

S

R

D

O

A

U

D

L P

T

E

X

R

T

B

E

T

E

3

I

P

R

M

D

S

A

I

U

E

S

E

S

A

V

I

O

I

L

T

P

T

C

H

X

E

G

E

J I B L

D

O

I

R

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E

M

S

V

O

A

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L

F

A V

O

I D

E X

P

O

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R

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E

R

L IG

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IS

F

E

R

M

O

IT

M

T

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T

D H IS

C

A

U

TI

P

O

E

N

R

–

T

C

U

L

R

A

S

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S

3

L

B A S

E R R

A D

IA T

IO N W

A

V

H O

E N

ID

O

EX

PE

P

N

O

S U

R E T

O

T

H E

B E A

M

V

O R

S IC H

T –

L A

S E

K

R

L

S A

T S

R

S

A

E

H

3

L U

B

N

,

G

W

E N

N A

B D

E C K

U N

N

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G T D

E

Ö

E

F

M

F

S

N

T

E

R

T A H L

AU

S S

ET Z E

N

Dia pillar illuminator

Filter cube port cover retaining plate

ON

OFF

6V30W

12V100W

MAX.

Safety label (7) (Both sides)

(7) IEC caution label -Class 3B areas

CAUTION – CLASS 3B LASER RADIATION WHEN OPEN AVOID EXPOSURE TO THE BEAM

–

VORSICHT KLASSE 3B, WENN ABDECKUNG GEÖFFNET NICHT DEM STRAHL AUSSETZEN

LASERSTRAHLUNG

(9) IEC aperture label 2

AVOID EXPOSURE

LASER LIGHT IS EMITTED

THIS APERTURE

FROM

Figure 1.4-50

The filter slider cover is a component designed for preventing unintended exposure of eyes or skin at the slider port to laser light emitted to the dia-pillar illuminator. To attach it to the Ti microscope, lay it over the dia-pillar illuminator and secure it with the screws and nuts provided with the kit.

Filter cube port cover retaining plate

The filter cube port cover retaining plate is a component designed for preventing accidental leakage of laser light upon occurrence of unintended opening of the filter cube port cover.

Attach it to the side of the epi-fl filter turret’s lower plate using the screws provided with the kit.

Safety labels

The label (7) affixed to the upper left side (when viewed from the Binocular eyepiece tube) of the dia pillar indicates that the laser light may come out from the aperture when the lamphouse is removed for replacement of the lamp inside.

Another labels (7) on both sides of the microscope base indicates that the laser light may come out from the aperture while attaching the epi-fl filter turret or the nosepiece to the microscope.

The labels (9) on both sides of the microscope base indicates that the laser light may come out of the analyzer port.

1-62

Chapter 1 System Configurations and Part Names

1.4 Name of Each Part

Protection plate (for nosepiece)

The protection plate (for nosepiece) is a component designed for shielding the gap between the nosepiece and the epi-fl filter turret to avoid accidental jamming of fingers inserted into the gap or the laser radiation from the gap.

Two types of protection plate are prepared for the nosepiece being used. One for the TI-N6, TI-ND6 and TI-ND6-E nosepieces is supplied with Ti-E and Ti-E/B, and the other for the TI-ND6-PFS nosepiece is supplied with the nosepiece. Follow the procedure described in “4.2.3 Attaching the Laser Safety Kit” to attach the protection plate in place.

1-63

Chapter 1 System Configurations and Part Names

w

1.4 Name of Each Part

1.4.7 Related Product

TI-BPU back port unit

Adding a camera port by attaching the TI-BPU back port unit to the microscope base allows for a concurrent image capture with two cameras.

Attach a C mount camera via a separate C mount adapter to the back port unit and perform focus adjustment and centering.

Important

The dichroic mirror inside the filter cube is used to split the optical path, therefore, the dichroic mirror with a poor flatness may cause lens effect due to a curved surface reflection, resulting in poor image formation.

Use a dichroic mirror with higher flatness to be used for optical path split.

C mount adapter clamp screws (two)

Focus knob

(separately available) C mount adapter

Clamp scre

Centering screws (two)

Portion to be inserted into the microscope

Figure 1.4-51

To eyepiece

1-64

Chapter 1 System Configurations and Part Names

1.4 Name of Each Part

TI-BSUK70 70 mm stage up kit

By attaching the TI-BSUK70 70 mm stage up kit and doubly mounting the epi-fl filter turrets, you can attach any of the TIRF illuminator unit/motorized TIRF illuminator unit/photo activation illuminator unit and TI-BPU back port unit simultaneously.

The unit that can be attached to the upper or lower tier is selectable in accordance with the intended use.

Important

The condensing position will be misaligned when the TIRF illuminator unit/motorized TIRF illuminator unit/photo activation illuminator unit is attached to the lower tier.

When attaching to the lower tier, embed the TI-TIRF stage up lens into the TIRF illuminator unit/motorized TIRF illuminator unit/photo activation illuminator unit to correct the condensing position and provide the optimum illumination performance.

Contact your own Nikon representative for details on the stage up lens.

The following figure shows the Ti-U with the stage up kit mounted, along with the TIRF illuminator unit on the upper tier and the back port unit on the lower tier.

Epi-fl filter turret (upper tier)

Epi-fl filter turret (lower tier)

TIRF illuminator unit

Back port unit

Figure 1.4-52

1-65

Chapter 1 System Configurations and Part Names

1.4 Name of Each Part

Positioning pin

Stage support block Nosepiece support block

Epi-fl filter turret

support block (left)

Positioning pin

Dia-illuminator pillar support block

TI-BSUK70 70 mm stage up kit

Shielding plate Epi-fl filter turret

Figure 1.4-53

support block (right)

Support pillar extension

1-66

2

Preparations

2.1 Check Items and Tools

2.1.1 Required Items

Make sure you have all of the following items before starting.

• PC (complete set)

• PC-related devices

• Operating system

• Laser: Recommended laser (see Chapter 1, “System Configuration and Part Names”)

• Camera system (for TIRF and PAU image observation)

• Dichroic mirror (when the photo activation illuminator unit is used only.)

• One power cable

one cable for the four-laser module: 100 VAC area: 125 VAC 7 A or higher; 200 VAC area: 250 VAC 6 A or higher

one cable for the AOM controller: 100 VAC area: 125 VAC 7 A or higher; 200 VAC area: 250 VAC 6 A or higher

two cables for the laser PS rack: 100 VAC area: 125 VAC 7 A or higher; 200 VAC area: 250 VAC 6 A or higher, and 100 VAC area: 125 VAC 15 A or higher; 200 VAC area: 250 VAC 10 A or higher

CAUTION

Be sure to prepare power cables satisfying the ratings as specified above and complying with the local safety standards. Extra care must be taken for the laser PS rack in particular, which has a great current capacity.

1-67

Chapter 2 Preparations

2.1 Check Items and Tools

2.1.2 Tools

The following are the tools required in the setup procedure. Before starting, make sure you have the following tools required to set up the system.

General tools

• Allen wrench (mm, inch)

• Phillips screwdriver

• Fluorescent slide specimen (you can also use an HE-stained specimen)

Special tools

• Laser-protection goggles • Calibrated power meter

Figure 2.1-1 Figure 2.1-2

• Power measurement attachment • Dichroic mirror centering tool

Figure 2.1-3 Figure 2.1-4

• Coupler-centering tool • Centering tool for visual observations and mirror for observing centering tool

Figure 2.1-5 Figure 2.1-6

• Nikon 2-piece M4 screwdriver set

(with red handles)

• Polarization orientation adjustment tool and polarizer-equipped tool

Figure 2.1-7 Figure 2.1-8

• AOTF driver remote controller (for maintenance) • Centering tool (left: for LU4A) * Two pieces of a type are required.

Figure 2.1-9 Figure 2.1-10

1-68

Chapter 2 Preparations

2.2 Overview of Setup Procedure

1

2

3

4

5

Setting Up the PC and

Operation Software

(Chapter 3)

Setting Up the Microscope

(Chapter 4)

Laser Unit (Chapter 5)

Connection between the Laser

Unit and the Microscope

Confirmation and

(Chapter 6)

Check Sheet

(Chapter 7)

• Attaching the light source (TI-TIRF, TI-TIRF-E, or TI-PAU)

• Attaching the dichroic mirror (TI-PAU only)

• Attaching the epi-fl filter turret (TI-FLC, TI-FLC-E, or TI-FLC-E/HQ)

• Assembling the microscope

• Attaching the field diaphragm unit

• Attaching the laser safety cover (TI-TIRF or TI-TIRF-E)

• Mounting the filter cubes

• Attaching the camera

• For two-laser unit and three-laser unit

• For three-laser unit EX

• For the four-laser module A

• For the four-laser PS rack

• Connection to the Shutter Unit

• Connection to the fiber light source (TI-TIRF, TI-TIRF-E, or TI-PAU)

• Replacing the optical path switch mirror (if necessary)

• Laser safety check

• Check sheet entry

1-69

3

Setting Up the PC and Operation Software

Using TIRF and PAU microscopy, images are observed on a monitor, via a camera attached to the microscope camera port. TIRF images and laser port images from PAU microscopy cannot be observed through the eyepieces. Make sure to set up the PC and operation software for the camera to be used.

1-70

4

Setting Up the Microscope

4.1 List of Parts

The parts below have been added to the basic set of the Ti-E, Ti-E/B, Ti-U, and Ti-U/B.

The procedures for attaching sub-units and parts required for installing the TIRF-PAU illuminator are described later in this section.

TIRF illuminator unit or motorized TIRF illuminator unit

Table 4.1-1

Unit Name of equipment Model Remarks

Ti-E

Microscope and accessories

TIRF/ motorized TIRF illuminator unit

Ti

Objectives for TIRF

Shutter Unit

AC adapter

Epi-fl filter turret for epi-fl illumination

Episcopic light source

Stage ring

Stage up kit TI-BSUK70

Back port unit TI-BPU

TIRF illuminator unit

Fiber C1 single mode fiber

Straight fiber adapter TIRF2 S

Stage up lens

Ti-E/B

Ti-U

Ti-U/B

Apo TIRF 60xH/1.49

Apo TIRF 100xH/1.49

Plan Apo TIRF 60xH/1.45

Plan Apo TIRF 100xH/1.45

Plan Apo VC 60xH/1.4

Plan Apo VC 100xH/1.4

TI-LUSU

TI-LU4SU

AD-1260B

EA1050E-120

TI-FLC

TI-FLC-E

TI-FLC-E/HQ

Fiber optic light source

Lamphouse + power supply

26

32

TI-TIRF

TI-TIRF-E

TI-TIRF Stage up lens

Use with the TI-DH dia-illuminator 100W.

Select the appropriate objective for observation.

PC compatible

For TI-LUSU shutter unit

Three exclusive high-precision filter cubes are supplied with the TI-FLC-E/HQ as accessories.

Make sure to read the instruction manuals for the product.

32 is made of glass.

TI-BSUK70 70 mm stage up kit is required.

TIRF100 and TIRF60 - EPI40 are interchangeable.

Straight fiber can be used combined with the straight fiber adapter.

When connecting an optical fiber that has a straight tip, install a straight fiber adapter to the attachment.

When using the TIRF/motorized TIRF illuminator unit on the lowest step during stageup, install the stage up lens to the TIRF/motorized TIRF illuminator unit. The TI-BSUK70 70 mm stage up kit is required to stageup.

1-71

Chapter 4 Setting Up the Microscope

4.1 List of Parts

Unit Name of equipment Model Remarks

Commerciallyavailable PC

Commerciallyavailable monitor

Commerciallyavailable TV camera

An adapter is required for connection to the microscope port.

See “Chapter 5 Laser Unit” for details on which lasers can be mounted.

TIRF image observation

Laser Unit

PC

Monitor

TV camera

Two-laser unit C-LU2

Three-laser unit C-LU3

Three-laser unit EX C-LU3EX

Four-laser module A LU4A

Filter slider cover

Ti laser safety kit

Filter cube port cover retaining plate

Safety label

Protection plate

1-72

Chapter 4 Setting Up the Microscope

Photo activation illuminator unit

Unit Name of equipment Model Remarks

Ti

Shutter Unit

AC adapter

Microscope and

accessories

Photo activation

illuminator unit

PA-GFP image

observation

Laser Unit

Epi-fl filter turret for epi-fl illumination

Episcopic light source

Stage ring

Stage up kit TI-BSUK70

Back port unit TI-BPU

Photo activation illuminator unit

Fiber C1 single mode fiber

PC Commercially-available PC

Monitor

TV camera

Two-laser unit C-LU2

Three-laser unit C-LU3

Three-laser unit EX C-LU3EX

Four-laser module A LU4A

Filter slider cover

4.1 List of Parts

Table 4.1-2

Ti-E

Ti-E/B

Ti-U

Ti-U/B

TI-LUSU

TI-LU4SU

AD-1260B

EA1050E-120

TI-FLC

TI-FLC-E

TI-FLC-E/HQ

Fiber optic light source

Lamphouse + power supply

26

32

TI-PAU

Commerciallyavailable monitor

Commerciallyavailable TV camera

Use with the TI-DH dia-illuminator 100W.

PC compatible

For TI-LUSU shutter unit

Make sure to read the instruction manuals for the product.

32 is made of glass.

TI-BSUK70 70 mm stage up kit is required.

Switches PA-GFP/EPI. Requires an optical path switch mirror.

An adapter is required for connection to the microscope port.

See “Chapter 5 Laser Unit” for details on which lasers can be mounted.

Ti laser safety kit

Filter cube port cover retaining plate

Safety label

Protection plate

1-73

Chapter 4 Setting Up the Microscope

4.2 Setup Procedure

4.2.1 Assembling Each Part and Attaching Them to the Microscope

Perform the steps below to assemble each part and attach them to the microscope.

Attach the light source (TIRF illuminator unit, motorized TIRF illuminator unit, or photo

1

activation illuminator unit).

⇒ Page 1-75

Attach the dichroic mirrors (photo activation illuminator unit only). ⇒ Page 1-77

2

Attach the field diaphragm unit. ⇒ Page 1-78

3

Attach the epi-fl filter turret. ⇒ Page 1-79

4

Assemble the remaining microscope parts. ⇒ Page 1-79

5

Attach the laser safety cover (TIRF illuminator unit or motorized TIRF illuminator unit

6

only).

Attach the light shielding plate. ⇒ Page 1-80

7

Attach the filter cubes. ⇒ Page 1-81

8

Attach the camera. ⇒ Page 1-81

9

⇒ Page 1-80

Affix the labels. ⇒ Page 1-82

10

1-74

r

Chapter 4 Setting Up the Microscope

4.2 Setup Procedure

Attach the light source (TIRF illuminator unit, motorized TIRF illuminator unit, or photo

1

activation illuminator unit).

1. Remove the black cover on the center of the

microscope base.

(1) Using an Allen wrench, remove the two

screws securing the black cover.

(2) Remove the black cover.

Remove the black cover

Insert the light source

Figure 4.2-1

Four clamp screws

2. Attach the light source (TIRF illuminator unit, motorized TIRF illuminator unit, or photo activation illuminator unit) to the microscope main body.

(1) Insert the light source into the guide on the

back of the microscope main body.

(2) Secure the light source by tightening the 4

clamp screws using the Allen wrench.

Figure 4.2-2

3. Attach the support pillar to the light source.

Support pilla

Figure 4.2-3

(1) Use an Allen wrench to loosen the clamp

screw securing the support pillar so that the sliding part at the end slides freely.

(2) Screw the support pillar into the mounting

hole on the bottom of the light source to attach it.

(3) Adjust the length of the support pillar so that

its tip touches the installation surface, and then tighten the clamp screw with the Allen wrench to secure it.

1-75

Chapter 4 Setting Up the Microscope

A

4.2 Setup Procedure

Laser position adjustment knob

Two clamping screws

Mercury episcopic light source adapter

Figure 4.2-4

Clamping hardware

Figure 4.2-5

Laser position adjustment knob

Anchoring bolt

Laser connection port (TI-PAU)

nchoring ring

Laser position adjustment knob

Laser connection port (TI-TIRF)

Figure 4.2-6

4. Connect the mercury episcopic light source

adapter (or mercury lamphouse) to the episcopic light-source port (bayonet ring)

Connect the episcopic light source on the side

where you attached the support pillar in step 3.

For details about attaching the light source, see

the instructions of your mercury lamp power supply or super-high pressure mercury lamp power supply.

5. Attach the clamping hardware to the episcopic light-source port.

Secure the clamping hardware by tightening the

two clamp screws using the Allen wrench.

The clamping hardware is provided to prevent the

PA-GFP irradiation attachment from being removed by mistake. Be sure to attach the screw for laser safety.

6. Remove the anchor on the laser side.

The laser position adjusting mechanism comes

equipped with an anchor for transportation. Be sure to remove the anchor before turning the laser position adjustment knobs.

Attempting to turn either of the knobs without

first removing the anchor may cause the mechanism to malfunction.

(1) For TI-PAU:

The two laser position adjustment knobs are

loosened before transportation. Turn both knobs gently clockwise until you feel resistance, and then remove the anchoring bolt.

(2) For TI-TIRF/TI-TIRF-E

The two laser position adjustment knobs are

loosened before transportation. Tighten both knobs by turning them gently clockwise until you feel resistance. Then, extract the two locking screws and remove the brass anchor ring.

1-76

Chapter 4 Setting Up the Microscope

4.2 Setup Procedure

Anchoring bolt

Attach the dichroic mirrors (photo activation illuminator unit only).

2

It is necessary to attach a dichroic mirror (optical path switch mirror), which switches between laser light and episcopic illumination, inside the photo activation illuminator unit. Since the attachment does not ship with a mirror attached, you must attach one following the procedure below.

You can also change the light intensity ratio of laser light and episcopic illumination by switching the dichroic mirror and operating the optical path switch lever.

Ordinarily, you should attach a total reflection mirror. When using a total reflection mirror, operating the optical path switch switches between 100% laser light and 100% episcopic illumination.

WARNING

Figure 4.2-7

7. Remove the anchoring bolt from the optical path

switching module.

The optical path switching module comes equipped with an anchor for transportation. Be sure to remove the anchor before use.

1. Unscrew the anchoring bolt from the top of

the optical path switching module.

2. To plug the hole where the anchoring bolt

was, screw in the button bolt provided.

To prevent hazardous laser emission from the mirror replacement opening, turn off the laser power before replacing mirrors.

Cap

Figure 4.2-8

1. Remove the retaining screw for the cap of the

mirror replacement opening using the Allen wrench, and then remove the cap by turning it counterclockwise.

1-77

Chapter 4 Setting Up the Microscope

w

4.2 Setup Procedure

Holder plate locking screws

2. Remove the mirror unit from inside the illuminator, and insert the dichroic mirror.

(1) When removing the mirror unit, pull out the

mirror switch lever, and then pull out the mirror unit while holding the tab of the mirror unit (magnetic lock).

Holder plate

(2) Remove the two holder plate locking

screws. Next, remove the holder plate and flat spring to attach the mirror.

Flat spring

• Mirror size: 36 x 25.7 x 1

Mirror (Place the reflection surface downward.)

3. Attach the mirror unit in its original position, and then close the cap of the mirror replacement opening.

(1) There is a positioning hole on the mirror

unit. Securely attach the mirror unit to the

Mirror unit

Figure 4.2-9

illuminator to fit the pin on the main body.

(2) Screw the cap of the mirror replacement

opening until it is closed.

(3) Tighten the optical path switching module

screw using the Allen wrench, and secure the cover.

Attach the field diaphragm unit.

3

Locking scre

Field diaphragm unit

Figure 4.2-10

1. Remove the rubber cap on the left side of the

microscope.

2. Insert the field diaphragm unit.

3. Tighten the locking screw using an Allen wrench,

and secure the field diaphragm unit.

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1-78

Chapter 4 Setting Up the Microscope

4.2 Setup Procedure

Attach the epi-fl filter turret.

4

Attach the epi-fl filter turret to the microscope main body.

Insert the epi-fl filter turret along the attachment groove from the right side of the microscope and secure with screws on the left and right. (Use an Allen wrench. Note that the screws should be tightened after the filter cube port cover is removed.)

Epi-fl filter turret

Insert from right

Filter cube port (and its cover)

Front side

Figure 4.2-11

When using the epi-fl filter turret in combination with the Ti-E or Ti-E/B, the protection plate (for nosepiece) must be installed between the epi-fl filter turret and the nosepiece.

* For installation procedure, see Section 4.2.3, “Attaching the Laser Safety Kit.”

Assemble the remaining microscope parts.

5

Refer to the instruction manual included with the microscope main body before assembling the remaining microscope parts.

1-79

Attach the laser safety cover (TIRF illuminator unit or motorized TIRF illuminator unit only).

6

Safety interlock (both sides)

Chapter 4 Setting Up the Microscope

4.2 Setup Procedure

If you are using the TIRF illuminator unit or the motorized TIRF illuminator unit, the motorized shutter will not open unless the laser safety cover is attached

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Laser safety cover

on the stage. Make sure it is attached in the prescribed position.

1. Place the laser safety cover body on the stage, and secure it by tightening the 4 screws with the

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6V30W

12V100W

MAX.

unit.

3. Attach the cover on top of the body.

The interlock will close the motorized shutter of the laser unit if the cover is removed during use.

* In the case of the photo activation illuminator unit,

Upper cover

you must attach a specimen cover on the stage when using episcopic illumination or laser light.

* Touching the interlocking part or getting it wet could

cause it to malfunction.

Body

Figure 4.2-12

Attach the light shielding plate.

7

B

Figure 4.2-13

Light shielding plate

Using an Allen wrench, screw the light shielding plate onto the eyepiece tube.

1-80

Chapter 4 Setting Up the Microscope

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APERTURE

4.2 Setup Procedure

Attach the filter cubes.

8

You can attach up to six filter cubes in the epi-fl filter turret.

Excitation method changeover ring

Address marking

Set the filter cube with its excitation filter facing outside. (Do not set it in opposite way.)

Filter cube port cover retaining plate

Indication of address on the optical path (position for ID sticker)

Filter cube

Figure 4.2-14

1. Remove the filter cube port cover, check the turret

address, and insert the filter cubes along the guide groove.

2. Rotate the filter cube rotation control to display the same address and affix an ID sticker that matches the filter cube type.

Affix

or (a blank sticker that can be written on freely) on spaces in which no filter cube is inserted.

3. Re-attach the filter cube port cover.

Note: Excitation and barrier filters can be removed

4. Attach the filter cube port cover retaining plate.

from the filter cube and replaced with other filters. (The filters are screwed into the filter cube.)

Important

Excitation filter

• Three exclusive high-precision filter cubes are supplied with the TI-FLC-E/HQ motorized epi-fl filter turret as accessories. These filter cubes are adjusted to the address of the epi-fl filter turret. Make sure to attach the exclusive high-precision filter cubes by matching the inscribed addresses.

• If you use an excitation filter attached to the excitation filter slider, attach the filter cube to the excitation filter.

Fixing screw

Figure 4.2-15

Attach the camera.

9

Attach the camera to the camera port.

1-81

Chapter 4 Setting Up the Microscope

4.2 Setup Procedure

10

Affix the labels.

After confirming the safety of the laser (p.1-228 to 1-230), affix the specified warning labels to the locations shown below.

Left side of microscope main unit

Affix “(7) IEC caution label - Class 3B area” on the left side of the microscope main unit, under the field diaphragm.

Both sides of microscope main body

Affix “(7) IEC caution label - Class 3B area” and “(9) IEC aperture label 2” above the right and left ports of the microscope main unit.

On 100W dia pillar illuminator

Affix

“(7) IEC caution label - Class 3B area” on the dia pillar illuminator 100W.

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(9) (both sides)

(7) (both sides)

Figure 4.2-16

1-82

Chapter 4 Setting Up the Microscope

y

r

A

4.2 Setup Procedure

On the laser safety cover (TIRF illuminator unit or motorized TIRF illuminator unit)

Make sure that the following three warning labels at the rear left, when viewed from the Binocular eyepiece tube: “(1) IEC warning label - laser hazard symbol”, “(2) IEC explanatory label - Class 3B laser product”, and “(8) IEC aperture label 1” is affixed.

(8)

(2)

LASER RADIATION AVOID EXPOSURE TO BEAM CLASS 3B LASER PRODUCT

AVOID EXPOSURE

LASER LIGHT IS EMITTED

OBJECTIVE APERTURE

FROM

(1)

Figure 4.2-17

Top surface of stage (photo activation illuminator unit)

Make sure that the following three warning labels at the rear left, when viewed from the Binocular eyepiece tube: “(1) IEC warning label - laser hazard symbol”, “(2) IEC explanatory label - Class 3B laser product”, and “(8) IEC aperture label 1” is affixed.

Laser

cove

safet

Stage top surface

(2)

bout 5 mm

About 5 mm

(8)

LASER RADIATION AVOID EXPOSURE TO BEAM CLASS 3B LASER PRODUCT

AVOID EXPOSURE

LASER LIGHT IS EMITTED

OBJECTIVE APERTURE

FROM

About 5 mm

(1)

Stage top surface

Figure 4.2-18

1-83

Chapter 4 Setting Up the Microscope

A

4.2 Setup Procedure

Laser unit

Remove the laser safety label if it is attached to the laser unit.

Remove the laser safety labels.

bout 12 mm

About 20 mm

Figure 4.2-19

About 30 mm

bout 12 mm

1-84

Chapter 4 Setting Up the Microscope

4.2 Setup Procedure

4.2.2 Attaching the N-STORM Kit

Install the following parts to the microscope to perform N-STORM microscopy.

Ti-E fixing plates

Attach plates to fix the microscope to the anti-vibration table.

Detach the front and rear rubber feet at the bottom of the microscope and secure the fixing plates (front and rear).

Install the microscope to the anti-vibration table according to the inch holes or centimeter holes.

(Front) (Rear)

Ti-E fixing plates

Figure 4.2-20

3D-STORM port

Attach an optical system for N-STORM to the microscope.

Cylindrical lens IN/OUT handle

OUT

IN

3D-STORM port

Detach the plastic cover on the side port of the microscope and install the 3D-STORM port. When using the port independently, install it in the left side port. When using the port together with the confocal microscope A1, install it in the right side port.

Note: The 3D-STORM port cannot be installed

in the bottom port or the back port.

3D-STORM port

Combination of A1 and 3D-STORM port

Figure 4.2-21

1-85

Chapter 4 Setting Up the Microscope

r

4.2 Setup Procedure

DSC support columns (2 pieces)

Attach supports to fix the camera to the anti-vibration table.

Screw the supports in the screw holes in the camera (Andor iXon) body.

Secure the camera to the side 3D-STORM port of the microscope and set the magnet base to ON.

DSC support columns

Figure 4.2-22

STORM slider

Insert the dedicated optical system into the TIRF optical system to perform N-STORM microscopy.

Detach the cover at the upper part of the laser

OUT

IN

STORM slider

introduction section in the motorized TIRF illuminator unit.

You can see a slot to insert the STORM slider. Insert the slider into the slot.

Then attach the cover to prevent the slider from coming off.

Insert this slider during N-STORM microscopy, but slide it out of the optical path in other cases.

Figure 4.2-23

Lambda plate slider

Insert an optical device for polarization adjustment to perform N-STORM microscopy.

Remove the stopper screw and detach the ND16 of

Lambda

plate slide

the motorized TIRF illuminator unit.

Insert the lambda plate slider into the same position. Install the stopper screw.

Insert this slider during N-STORM microscopy, but slide it out of the optical path in other cases.

Figure 4.2-24

1-86

Nikon Eclipse TI-TIRF-E, Eclipse TI-PAU, Eclipse TI-TIRF, Eclipse Ti, LU4-B5 Setup Manual

Introduction

Safety Precautions

How to Use This Manual

Contents

System Configurations and Part Names

1.1 Overview

1.1.1 TIRF Illuminator Unit and Ti-U System Overview

1.1.2 Motorized TIRF Illuminator Unit and Ti-E System Overview

1.1.4 TI-PAU Photo Activation Illuminator Unit and Ti-U System Overview

1.2 Optical Paths of Lasers

1.2.1 Laser Unit

1.2.2 Microscope Part (TIRF Illuminator Unit and Ti-U System)

1.2.3 Microscope Part (Motorized TIRF Illuminator Unit and Ti-E System)

1.2.5 Microscope Part (Photo Activation Illuminator Unit and Ti-U System)

1.3 Safety Labels and Openings

1.3.1 Ti-U Microscope Body (with the TIRF Illuminator Unit)

1.3.2 Ti-E Microscope Body (with the Motorized TIRF Illuminator Unit)

1.3.3 Ti-U Microscope Body (with the TI-PAU Photo Activation Illuminator Unit)

1.3.4 C-LU2 Two-laser Unit

1.3.5 C-LU3 Three-laser Unit

1.3.6 C-LU3EX Three-laser Unit EX

1.3.7 LU4A Four-laser Module A

1.3.8 LU-LR Four-laser PS Rack

1.3.9 TI-LUSU Shutter Unit

1.3.10 TI-LU4SU Shutter Unit LU4

1.3.11 TI-TIRF TIRF Illuminator Unit

1.3.13 TI-TIRF-E Motorized TIRF Illuminator Unit ― For N-STORM Microscopy

1.3.14 TI-PAU Photo Activation Illuminator Unit

1.4 Name of Each Part

1.4.1 TIRF Illuminator Unit and Ti-U System

1.4.2 Motorized TIRF Illuminator Unit and Ti-E System

1.4.3 Motorized TIRF Illuminator Unit, LU4A, N-STORM and Ti-E System

1.4.4 TI-PAU Photo Activation Illuminator Unit and Ti-U System

1.4.5 Laser Unit

1.4.6 Laser Safety Kit

1.4.7 Related Product

Preparations

2.1 Check Items and Tools

2.1.1 Required Items

2.1.2 Tools

2.2 Overview of Setup Procedure

Setting Up the PC and Operation Software

Setting Up the Microscope

4.1 List of Parts

4.2 Setup Procedure

4.2.1 Assembling Each Part and Attaching Them to the Microscope

4.2.2 Attaching the N-STORM Kit