Nikon ECLIPSE E200 POL Instructions Manual

Microscope

ECLIPSE E200 POL

Instructions

M260E 04.2.CF.3(1/2)

Thank you for purchasing this Nikon product. This instruction manual is for the users of the Nikon Microscope ECLIPSE E200 POL describing basic operation of the microscope. To ensure correct usage, please read this manual thoroughly before using the microscope.

• It is prohibited to reproduce or transmit this manual in any form without the prior consent of Nikon.

• The instructions and specifications in this manual are subject to change without notice.

• Although every effort has been made to ensure the accuracy of this manual, if you find that any part of

this manual is unclear or incorrect, contact your nearest Nikon representative.

• Some ECLIPSE E200 POL microscope sets may come with different components and accessories from those shown in this manual.

• Also read the manuals for the products used with the microscope, for example, the Nikon photomicrographic equipment.

Safety Precautions

Warning and Caution Symbols Used in This Manual

Though Nikon products are designed to provide you with utmost safety during use, incorrect usage or disregard of the instructions may cause personal injury or property damage and will lead to the forfeiture of all claims against warranty. For your own safety, read the instruction manual carefully and thoroughly before using the product. Do not discard this manual. Always keep it near the product for easy reference. Inside this instruction manual, safety instructions are indicated with the symbols shown below. Be sure to follow the instructions marked with these symbols for your safety.

Symbol Meaning

Disregarding instructions marked with this symbol may lead to death or serious injury.

Disregarding instructions marked with this symbol may lead to injury or property damage.

Meaning of Symbols Used on the Equipment

The symbol appearing on the product indicates the need for caution at all times during use. Always refer to the instruction manual and read the relevant instructions before manipulating any part to which the symbol has been affixed.

Symbol Meaning

Caution! Biohazard

This symbol label attached on the stand reminds you of the following:

• WARNING: Contact of a living body sample to the microscope, presents a biohazard risk.

• To avoid biohazard contamination, do not touch the contaminated portion with your bare

hands.

• Decontaminate the contaminated portion according to the standard procedure of your laboratory.

Caution for heat

This symbol label attached near the field lens unit (the lamp is set underneath the field lens unit) reminds you of the following:

• Lamp and its surrounding areas (including the field lens unit) become very hot during and immediately after illumination.

• Risk of burns. Do not touch the lamp or surrounding areas during and immediately after illumination.

• Make sure the lamp and its surrounding areas have cooled sufficiently before attempting to replace the lamp.

Caution

This symbol label attached near the AC inlet reminds you of the following:

• Check the input voltage before turning on the microscope. (The input voltage is given on the "nameplate" and above the AC inlet.)

• If the input voltage shown differs from the local voltage level, do not turn on the microscope. Do the following instead:

Different voltage on the nameplate Contact your nearest Nikon representative.

Different voltage above the AC inlet Change the input voltage setting; refer to P.35.

WARNING

CAUTION

Safety Precautions

1. Intended use of the equipment

This microscope is intended mainly for use in microscopic observation of rock and mineral or polymer materials or substance of living body, using polarized light illumination. It is designed for the main purposes of analysis of the optical character, in laboratories or hospitals, of such samples within the fields of mineralogy, high polymer chemistry, medicine.

2. Do not disassemble.

Disassembly may cause malfunction and/or electrical shock, and will lead to the forfeiture of all claims against warranty. Do not disassemble any part other than those described in this manual. If you experience any problem with the microscope, notify your nearest Nikon representative.

3. Check the input voltage.

The input voltage is indicated in two places at the rear of the microscope: on the nameplate and above the AC inlet. Confirm that these input voltage indications correspond to the voltage provided in your region. If not, follow one of the instructions below. The use of microscopes with the different input voltage indications will cause overcurrent and overheating, which may result in fire or severe damage to the microscope.

• If the voltage indication on the nameplate differs:

Î Do not plug in the microscope. Contact your nearest

Nikon representative.

• If the voltage indication above the AC inlet differs:

Î Refer to p. 35 and change the input voltage

setting before turning on the power switch.

• For the microscope with the nameplate showing [100/110/120 V ~]: The voltage can be set to: AC 100 V, 110 V or 120 V.

• For the microscope with the nameplate showing [220/230/240 V ~]: The voltage can be set to: AC 220 V, 230 V or 240 V.

4. Use the specified lamp, fuse, and power cord.

Use the specified lamp and fuse. Use the power cord provided. Using an incorrect lamp, fuse, or power cord may damage the instrument or cause a fire. (Also see p. 50 on power cord.) If using an extension cord, only use a cord that includes a protective earth (PE) wire.

• Specified Lamp

Halogen lamp 6 V-20 W (PHILIPS 7388 or OSRAM HLX64250) or

Halogen lamp 6 V-30 W (PHILIPS 5761)

• Specified Fuse

250 V, 1 A, time-lag low-breaking type, 5x20 miniature fuse x2

Input Voltage Indications

WARNING

Safety Precautions

5. Heat at the light source

The lamp becomes hot during use. Do not remove the field lens unit while the lamp is on, and be sure the lamp has been off for 30 minutes before touching it.

• When changing the lamp bulb, make sure that the lamp is cool enough to touch (the light should be

off at least 30 minutes).

• Do not touch the lamp while it is on or until the lamp has been off for 30 minutes, as doing so could

result in burns.

• Never bring cloth, paper or flammable volatile substances such as gasoline, petroleum benzine,

acetone, thinner, or alcohol near a hot lamp, as a fire could result.

6. Hazardous sample

This microscope is mainly for use in microscopic observation of rock and mineral or polymer materials or substance of living body, using polarized light illumination. When handling a living body sample, check to determine whether the sample is hazardous. Handle hazardous samples according to the standard procedure of your laboratory. If the sample is of an infectious nature, wear rubber gloves to avoid infection, and be careful not to touch a sample. In the event of contact of a sample to the microscope, decontaminate the contaminated portion according to the standard procedure of your laboratory.

1. Turn off power switch before assembling the microscope, replacing the lamp or fuse, and plugging in or unplugging the power cord.

Turn off the power switch before you plug or unplug the power cord to prevent electrical shock or fire. Also turn off the power switch and then unplug the power cord before assembling the microscope, and before changing the lamp or fuse. To turn off the power, turn the power switch to {.

2. Keep the microscope free of moisture and foreign matter.

Keep the microscope free of moisture to prevent short circuiting that could result in overheating or other malfunctions. If water splashes on the microscope, immediately turn off the power switch (turn the switch to {) and unplug the power cord. Then, wipe off the water with a dry cloth. Short circuiting can also result when foreign matter is trapped inside the microscope. If foreign matter or water has entered the microscope, do not use the microscope and contact your nearest Nikon representative.

3. Disposal of the microscope

To avoid biohazard risk, dispose of the microscope as the contaminated equipment according to the standard procedure of your laboratory.

WARNING

CAUTION

Safety Precautions

Notes on Handling the System

（1）Installation

This microscope is a precision instrument. Using the microscope in an unfavorable environment could result in malfunctions or degraded performance. Consider the following conditions when choosing the installation location.

• Observation conditions are better if light from windows and bright room light can be avoided.

• Install the microscope in a location with a room temperature of 0° to 40°C and with a

maximum relative humidity of 85%. High temperature and humidity are to be avoided because they promote mold growth and condensation, which may damage the microscope.

• Dirt and dust degrade optical performance and are to be avoided.

• Vibrations in the environment will degrade the image. Install the microscope in a location

free of vibrations.

• Install the microscope on a solid table and keep the microscope level.

• Select a layout that allows easy detachment of the power cord from the AC inlet of this

microscope in the event of emergency.

• This microscope emits a weak electromagnetic wave. Do not place a precision electronic device near the microscope as precision could be degraded. Also, avoid placing a radio or TV near the microscope as reception of sound and images may be hampered.

（2）Carrying the Microscope

This microscope is a precision instrument. Handle it gently. Strong shocks and forcible operation will damage the instrument. Shocks to the objectives, especially, could degrade image precision.

• When carrying the microscope, hold it at its upper rear and lower front ends.

• Do not hold the focus knobs, the eyepiece tube, or the stage. These parts could easily come off and could result in malfunctions.

（3）Handling the Lamp

Do not touch the glass part of the lamp with bare hands. Wear gloves or use a cloth when handling the lamp so as not to leave fingerprints on the surface. Wipe off any fingerprints or stains using a clean cloth moistened with alcohol. Fingerprints will etch into the hot surface of the lamp and reduce the brightness, damage the lamp or reduce its service life.

Handle the lamp gently. Shocks and vibrations will damage the lamp or reduce its service life.

When changing the lamp, be sure that the contact is not damaged. If the contact is damaged, the lamp may not light up or may overheat. Insert the lamp’s contact pins fully into the socket holes. If the pins are loose, the lamp could come off or result in a contact failure, which will cause overheating or smoke. Also, make sure that the field lens unit is securely attached.

Do not break the used lamps; instead dispose of them as special industrial waste or according to the laws applicable to your municipal waste system.

Safety Precautions

Notes on Handling the System

（4）Refocusing

When changing specimens using the refocusing mechanism, gently lower the stage by hand taking care not to hit the field lens with the condenser holder (p. 15).

（5）Focus Knobs

Do not turn the right and left focus knobs simultaneously in opposite directions. Do not turn the coarse focus knob any further after the stage has been moved up or down to its limit. These operations will damage the focusing mechanism. (The coarse focus knob has a protection device. The knob turns freely for a while after it has reached its upper limit.)

（6）Oil-Immersion Observation

Use only a minimum quantity of oil. If too much oil is applied, surplus oil could flow out to the stage and the condenser which could lead to degraded performance.

When using petroleum benzine or absolute alcohol to wipe off immersion oil or to clean the lenses, follow the instructions provided by their manufacturers. Absolute alcohol and petroleum benzine are inflammable. Take great care when handling them.

（7）Shipping Clamps

The microscope is held tightly by the clamps during shipment. Be sure to remove the clamps before use. For details, see p. 35.

Make sure not to hit the field l

ens

Do not turn the knobs in opposite directions.

Shipping Clamps

Shipping Clamps Shipping Clamps

WARNING

Contents

Safety Precautions .............................................................................................. 2

I Nomenclature of Each Part............................................................................. 8

II Switches and Controls..................................................................................10

III A Quick Microscopic Procedure.......................................................................12

IV Microscopy (Detailed Procedure) ....................................................................14

V Miscellaneous Operations..............................................................................31

1 Oil-Immersion Observation ................................................................................... 31

2 Adjusting the Torque of the Coarse Focus Knob....................................................... 32

3 Upper Limit Bolt .................................................................................................. 33

4 Cord Hangers...................................................................................................... 33

VI Assembly ...................................................................................................34

1 Assembly ........................................................................................................... 34

1) Input Voltage Check ...................................................................................... 34

2) Removal of Shipping Clamps .......................................................................... 35

3) Attaching the Intermediate Tube ..................................................................... 36

4) Attaching the Eyepiece Tube and the Eyepieces................................................. 36

5) Attaching the Analyzer Slider.......................................................................... 37

6) Attaching the Objectives ................................................................................ 37

7) Attaching the Condenser ................................................................................ 38

8) Connecting the Power Cord ............................................................................ 38

9) Other Accessories ......................................................................................... 38

2 Replacement of Consumable Materials ................................................................... 39

1) Replacing the Lamp ....................................................................................... 39

2) Replacing the Fuse ........................................................................................ 41

VII Optical Characteristics..................................................................................42

VIII Troubleshooting tables .................................................................................44

IX Care and Maintenance..................................................................................49

X Technical Specifications ................................................................................50

Nomenclature of Each Part

The microscope is made up of the following components.

(1) Basic unit

(2) Eyepieces

(3) Eyepiece tube

(4) Objectives

Objectives with various magnifying powers are available.

(5) Condenser

Used for condensing light. The condenser should be positioned slightly lower than its upper limit. Adjust the aperture diaphragm ring according to the objective.

(6) Polarizing intermediate tube

Used with the analyzer slider and the P-CL plate attached. Equipped with the Bertrand lens.

(7) Polarizer

Adjust the vibration direction together with the analyzer before use.

(8) Field lens unit

Draw out the field lens unit when changing lamp.

(9) Lamp

Halogen lamp 6 V-20 W or 6 V-30 W is used.

(10) Fuse

Two 250 V 1 A time-lag low-breaking type fuses are used.

(11) Power cord

Use the power cord provided.

I Nomenclature of Each Part

(2)

Eyepieces

(6)

Polarizin

intermediate

(4)

ectives

(5)

Condenser

(7)

Polarizer

(8)

Field lens unit

(9)

Lam

(3)

yep

iece tube

(1)

Basic unit

(10)

Fuse

(x2)

(11)

Power cord

Switches and Controls

(1) Diopter ring

Adjust the diopter ring to compensate for the difference between your right and left eyesight.

(2) Bertrand lens turret

Insert the Bertrand lens into the optical path when performing the conoscopic observation.

(3) Bertrand lens focus ring

Used to focus on the conoscopic image.

(4) Revolving nosepiece

Holds up to four objectives.

(5) Standard screw hole

mark

The marked objective screw hole is the standard hole of the nosepiece. Attach here, the objective with the highest magnification.

(6) Specimen clip

Holds the specimen.

(7) Condenser aperture scale

Indicates the numerical aperture.

(8) Swing-out condenser

Swing-out the top lens when performing the orthoscopic observation with the 4x objective.

(9) Condenser aperture

diaphragm ring

Usually, set to 70 to 80% of the numerical aperture of the objective.

(10) Polarizer

Insert into the bottom of the condenser.

(11) Stage rotation clamp

screw Fixes the stage in position.

(12) P-CL 1/4 λ and tint plate

Insert into the polarizing intermediate tube.

(13) Eyepiece tube clamp

screw

Turn with the supplied hexagonal wrench to fix the eyepiece tube.

(14) Analyzer slider

Insert into the polarizing intermediate tube.

(15) Intermediate tube clamp

screw

This is the eyepiece tube clamp screw of the microscope. It is used to fix the intermediate tube in place.

(16) Orientation plate

Shows the vibration directions of the polarizer and the analyzer.

(17) Circular graduated stage

Rotates 360 degrees. Equipped with the graduations which equally divides the circumference in

360.

(18) Power switch

Press the l side to turn on the power and to light the lamp. Press the { side to turn off the power and to distinguish the lamp.

(19) Brightness control dial

Turn clockwise to increase the voltage to make the viewfield bright. Turn counterclockwise to decrease the voltage and to darken the viewfield.

(20) Fine focus knob

Used for focusing. There is no coarse focus knob on the opposite side of the condenser focus knob.

(21) Condenser top lens

swing-out knob

Used to swing-out the top lens of the condenser.

(22) Vernier

Enables to readout the angles in 0.1 degrees.

(23) Bertrand lens centering

screw

Used to center the conoscopic image.

(24) Coarse focus knob torque

adjustment ring

Used to adjust the tension (torque) of the coarse focus knob.

(25) Coarse focus knob

There are both coarse and fine focus knobs on the side with the condenser focus knob.

(26) Condenser focus knob

Located on the same side as the coarse stage focus knob.

(27) Condenser clamp screw

(28) Nameplate

Indicates the input voltage.

(29) Voltage selector (Fuse

holder)

Match with the voltage provided in your region.

(30) AC Inlet

Plug in the power cord. Turn off the power switch before plugging in the power cord.

II Switches and Controls

(23)

Bertrand lens centerin

screw

(24)

Coarse focus knob torque adjustment ring

(25)

Coarse focus knob

(20)

Fine focus knob

(26)

Condenser focus knob

(27)

Condenser clamp screw

(28)

Name

late

(29)

Voltage selector (Fuse holder)

(30)

AC Inlet

(1)

Dio

ter ring

(2)

Bertrand lens turret

(3)

Bertrand lens focus rin

(4)

Revolvin

nosepiece

(5)

Standard screw hole mark

(6)

ecimen clip

(7)

Condenser a

erture scale

(8)

Swin

-out condenser

(9)

Condenser a

erture diaphragm ring

(10)

Polarizer

Stage rotation clamp screw

(12)

P-CL 1/4 λ and tint plate

(13)

Eyepiece tube clamp screw

(14)

Anal

zer slider

(15)

Intermediate tube clamp screw

(16)

Orientation

late

(17)

Circular graduated stage

(18)

Power switch

(19)

Brightness control dial

(20)

Fine focus knob

(21)

Condenser top lens swing-out knob

(22)

Vernier

(11)

A Quick Microscopic Procedure

III

Turn on the lamp and adjust interpupillary distance.

Focus with 10x objective.

Turn on the power switch.

Push the analyzer slider left.

Turn to position “0”.

Widen or narrow to merge the viewfields into one.

Adjust brightness too.

Viewfields

Match the bottom ed

e of the diopter ring with the engraved base line.

Place specimen slide on the stage.

(

Coverglass up.)

Swing the 10x in the optical path.

Slightly lower the condenser from its uppermost position.

Use this knob for focusin

III A Quick Microscopic Procedure

III

Use your right eye.

And focus on the crosshairs with this ring.

Use your right eye.

Use your left eye.

And focus with this ring.

And focus on both the crosshairs and the specimen.

Check the magnifying power.

Move the ring to the 70-80% of the objective. N.A.

Observe.

Turn off the power.

Wait till the microscope cools down before storing.

djust the diopter.

Magnify the image and observe!

Microscopy (Detailed Procedure)

Lamp Illumination

Turn on the power switch (turn to |) and the lamp will come on. Turn the brightness control dial to adjust the brightness of the viewfield. (Turning the dial clockwise increases the brightness; turning the dial counterclockwise decreases it.)

Interpupillary Distance Adjustment

Adjust the distance between the eyepieces to merge the right and left viewfields into one. (This is an adjustment to match the distance between eyepieces with the distance between your eyes).

Align the Diopter Ring with the Engraved Base Line

Turn the diopter ring on the right eyepiece to align its bottom edge with the engraved base line. Turn and align the diopter ring on the left eyepiece in the same way.

Specimen Mounting

Place specimen slide on the stage with the coverglass facing upward and fix with the specimen clips.

Power ON

Brightens.Darkens.

Viewfields

Merge the right and left viewfields into one.

Match the bottom edge of the diopter ring with the engraved base line.

+ 37 hidden pages

Nikon ECLIPSE E200 POL Instructions Manual

Specifications and Main Features

Frequently Asked Questions

User Manual