Page 1
Leica Z6 APO A
Leica Z16 APO A
User Manual
Page 2
General Instructions
Safety Concept
Before using your macroscope for the first time,
please read the "Safety Concept" brochure
included with your instrument. It contains additional information about handling and care.
Use in clean rooms
The Leica Z series can be used in clean rooms
without any problems.
Cleaning
★ Do not use any unsuitable cleaning agents,
chemicals or techniques for cleaning.
★ Never use chemicals to clean colored
surfaces or accessories with rubberized
parts. This could damage the surfaces, and
specimens could be contaminated by abraded particles.
★ In most cases, we can provide special solu-
tions on request. Some products can be
modified, and we can offer other accessories for use in clean rooms.
Servicing
★ Repairs may only be carried out by Leica
Microsystems-trained service technicians.
Only original Leica Microsystems spare
parts may be used.
Responsibilities of person in charge of
instrument
★ Ensure that the Leica macroscope is opera-
ted, maintained and repaired by authorized
and trained personnel only.
Leica Z6 APO A / Z16 APO A User Manual 2
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Important Safety Notes
User Manual
The individual modules of the Leica Z series
include an interactive CD-ROM with all relevant
user manuals in other languages. Keep it in a
safe place, and readily accessible to the user.
User manuals and updates are also available for
you to download and print from our web site
www.leica-microsystems.com.
This User Manual describes the special functions of the individual modules of the LeicaZ
series and contains important instructions for
their operational safety, maintenance, and
accessories.
The "Safety Concept" booklet contains additional safety information regarding the service
work, requirements and the handling of the
macroscope, accessories and electrical accessories as well as general safety instructions.
You can combine individual system articles
with articles from external suppliers (e.g. cold
light sources, etc.). Please read the user manual
and the safety requirements of the supplier.
Before installing, operating or using the instruments, it is mandatory to read the user manuals
listed above. In particular, please observe all
safety instructions.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
Leica Z6 APO A / Z16 APO A User Manual 3
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Symbols Used
Warning of a danger
This symbol indicates especially important information that must be read and
complied with.
Failure to comply can cause the following:
★ Hazards to personnel
★ Functional disturbances or damaged inst-
ruments
Warning of hazardous electrical voltage
This symbol indicates especially important information that is mandatory to
read and observe.
Failure to comply can cause the following:
★ Hazards to personnel
★ Functional disturbances or damaged inst-
ruments
Danger due to hot surface
This symbol warns against touching
accessible hot surfaces, e.g. those of
light bulbs.
Important information
This symbol indicates additional information or explanations that intend to
provide clarity.
Explanatory notes
★ This symbol within the text stands for addi-
tional information and explanations.
Figures
(1) Numbers in parentheses within the
descriptions relate to the figures and the
items within those figures.
Leica Z6 APO A / Z16 APO A User Manual 4
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Safety Instructions
Description
★ The individual modules fulfill the highest
requirements for observation and documentation with the Leica Z series.
Intended use
★ Refer to "Safety Concept" booklet.
Non-intended use
★ Refer to "Safety Concept" booklet.
Never use Z series macroscopes or their components for surgical procedures (for example, on
the eye) unless they are specifically intended
for that purpose.
The instruments and accessories described in
this operating manual have been tested for
safety and potential hazards. The responsible
Leica affiliate must be consulted whenever
the instrument is altered, modified or used in
conjunction with non-Leica components that
are outside of the scope of this manual.
Unauthorized alterations to the instrument or
noncompliant use shall void all rights to any
warranty claims.
Place of use
★ Refer to "Safety Concept" booklet.
★ Electrical components must be placed at
least 10 cm away from the wall and from
flammable substances.
★ Avoid large temperature fluctuations,
direct sunlight and vibrations. These conditions can distort measurements and micrographic images.
★ In warm and warm-damp climatic zones,
the individual components require special
care in order to prevent the build-up of
fungus.
Responsibilities of person in charge of
instrument
★ Refer to "Safety Concept" booklet.
Ensure that:
★ The Z series macroscopes and accessories
are operated, maintained and repaired by
authorized and trained personnel only.
★ All operators have read, understood and
observe this User Manual, and particularly
the safety regulations.
Repairs, service work
★ Refer to "Safety Concept" booklet.
★ Only original Leica Microsystems spare
parts may be used.
★ Before opening the instruments, switch off
the power and unplug the power cable.
★ Touching the live circuit can cause injury.
Leica Z6 APO A / Z16 APO A User Manual 5
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Safety Instructions (cont'd.)
Transport
★ Use the original packaging for shipping or
transporting the individual modules and
the accessory components.
★ In order to prevent damage from vibra-
tions, all moving parts that (according to
the user manual) can be assembled and
disassembled by the customer should be
disassembled and packed separately.
Integration in third-party products
★ Refer to "Safety Concept" booklet.
Disposal
★ Refer to "Safety Concept" booklet.
Legal requirements
★ Refer to "Safety Concept" booklet.
EC Declaration of Conformity
★ Refer to "Safety Concept" booklet.
Health risks
Workplaces equipped with macroscopes facilitate and improve the viewing task, but they
also impose high demands on the eyes and
holding muscles of the user. Depending on the
duration of uninterrupted work, asthenopia
and musculoskeletal problems may occur. For
this reason, appropriate measures for reduction
of the workload must be taken:Optimal arrangement of workplace, work assignments and
work flow (changing tasks frequently).
★ Thorough training of the personnel, giving
consideration to ergonomic and organizational aspects.
The ergonomic design and construction of the
Leica Z series are intended to reduce the exertion of the user to a minimum.
Direct contact with eyepieces can be a potential transmission path for bacterial and viral
infections of the eye. The risk can be kept to a
minimum by using personal eyepieces for each
individual or detachable eyecups.
Leica Z6 APO A / Z16 APO A User Manual 6
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Safety Instructions (cont'd.)
Light sources: Safety Instructions
Protective measures of the manufacturer:
★ UV protection screen in front of the speci-
men plane prevents the user from looking
directly into the UV rays.
★ Dummy filter carriers in the free positions
of the rapid filter changer prevent direct UV
radiation from reaching the eyes.
★ UV filters are installed in the observation
beam paths to protect the eyes.
★ The stray-light protection on the lamp
housing prevents irradiation of the hands.
Warning
UV radiation could damage the eyes. Therefore:
★ Never look into the light spot on the speci-
men plane without a UV protection screen.
★ Never look into the eyepieces if no excita-
tion filter is in the beam path.
★ Fill empty filter positions with dummy filter
carriers.
★ Do not select a white, strongly reflective
background for the specimen.
Supply unit
Always unplug the supply unit from the power
supply:
★ When installing and disassembling the
lamp housing
★ Before opening the lamp housing
★ When replacing the high-pressure mercury
lamp and other parts, such as the heatabsorbing filter or the collector
★ During maintenance work on the supply
unit
Lamp housing
★ Never open the lamp housing while the
lamp is switched on. Risk of explosion, UV
exposure, blinding!
★ Before opening the lamp housing, allow it
to cool off for at least 15 minutes. Danger
of explosion!
★ Never cover the air duct on the lamp
housing. Danger of fire!
Leica Z6 APO A / Z16 APO A User Manual 7
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Safety Instructions (cont‘d.)
Mercury lamp
★ Follow the user manual and safety instruc-
tions provided by the lamp manufacturer, and, in particular, the section on how
to proceed if a lamp breaks and releases
mercury.
★ For transport, remove the mercury lamp,
transport it in its original packaging and
protect moving parts in the lamp housing
using the transport anchors.
★ When it has reached the end of its rated life
(follow the manufacturer's specifications
and the minute meter on the supply unit).
★ To minimize the risk of explosion, replace a
discolored mercury lamp promptly
★ Leica Microsystems assumes no liability
for damage caused by exploding, incorrectly installed or improperly used mercury
lamps.
Leica Z6 APO A / Z16 APO A User Manual 8
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Table of Contents
General Instructions 2
Important Safety Notes 3
Symbols Used 4
Safety Instructions 5
The Leica Z Series
Congratulations! 13
The Modular Design: Everything is Relative 14
The Automatic Control System 15
On We Go 16
Assembly
Incident-light Bases and Manual Focusing Column 18
Transmitted-light Bases and Manual Focusing Column 19
Microscope Carrier 20
Video/Phototube A 21
Video/Phototube AS ("Slim") 22
Video/Phototube Y 23
Coaxial Illuminator (Optional) 24
Optics Carrier 25
Objective 26
Ergo binocular tube 27
Eyepieces 28
Transmitted-light Base TL ST 29
Transmitted-light Base TL BFDF: Before First Use 30
Transmitted-light Base TL BFDF 31
TL RC™ / TL RCI™ 32
IsoPro™ Manual Mechanical Stage: Assembly 33
Leica IsoPro™ Motorized Mechanical Stage: Assembly 36
Cables: Connections 40
Cables: Cable Duct 41
Motorized Focus: Adjusting the Travel Path 42
Cables: Diagram 43
Leica LED3000 NVI™ (Near Vertical Illumination) 46
Quick Start Guide
Overview of an automated Leica Z Series Macroscope (Leica Z16 APO A)
48
Eyepieces
Magnification Factors of the Eyepieces 50
Using the Eyepieces 51
The Correct Interpupillary Distance 52
Dioptric Correction 53
Graticules 54
Health Notes 55
Leica Z6 APO A / Z16 APO A User Manual 9
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Photography & Video
Photography & Video 57
Photo Tubes and C-mounts 58
Trinocular Video/Phototube 50% 59
Trinocular Video/Phototube 100% 60
Objectives and
Optical Accessories
The Different Types of Objectives 62
Bases
Leica TL ST Transmitted-light Base: Controls 64
Leica TL ST Transmitted-light Base: Operation 65
Leica TL ST Transmitted-light Base: Changing Bulbs 66
Leica TL BFDF Transmitted-light Base: Controls 67
Leica TL BFDF Transmitted-light Base: Operation 68
Leica TL RC™ / TL RCI™: Controls 69
Leica TL RCI™: The Deflection Mirror 70
Leica TL RCI™: Color Intensity and Temperature 71
Leica TL RC™ / TL RCI™: Operation 72
Leica TL RCI™: Methods in Transmitted Light 73
Leica TL RCI™: Relief Images 74
Using Filters 76
Leica IsoPro™ (Non-motorized): Controls 77
Leica IsoPro™ (Motorized): Controls 78
System Illumination
Leica LED3000 NVI™ (Near Vertical Illumination) 80
Accessories
Leica PSC Controller 82
Leica SmartTouch 83
Manual Control 84
Footswitch 85
Dimensional Drawings
Leica Z6 APO A 87
Leica Z16 APO A 88
Objectives 89
Video/phototube AS 90
Y tube 91
Video/Phototube A 92
Microscope carrier 93
Focusing arm for probers 94
Focusing arms 95
Adapters 96
Coaxial incident-light housing 97
Leica Z6 APO A / Z16 APO A User Manual 10
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Specications
Leica Z6 APO A & Z16 APO A 99
Optical Accessories for Leica Z6 APO A & Z16 APO A 100
Ambient Conditions and Weights 101
Optical Data – Visual with Y Tube 1.25× 102
Leica TL ST Transmitted-light Base 105
Leica TL BFDF Transmitted-light Base 106
Leica TL RC™ / TL RCI™ 107
Leica IsoPro™ Motorized XY Stage 108
Appendix
Calculating the Total Magnification and Field of View Diameter 110
Care, Maintenance, Contact Persons 111
Leica Z6 APO A / Z16 APO A User Manual 11
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The Leica Z Series
Leica Z6 APO A / Z16 APO A User Manual 12
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Congratulations!
Congratulations on obtaining your new Leica Z series macroscope. We are
convinced it will exceed your expectations, as the Z series embodies all
the qualities you associate with the name Leica Microsystems: excellent
objectives, high-quality engineering, and reliability. Furthermore, the
modular design ensures that the Z series adapts perfectly to your needs
—no matter which accessories you require for your tasks.
Thanks to the parallax error free observation, measurements are more
accurate and adaptation tasks are easier. Together with a Leica digital
camera, you obtain the maximum amount of high-quality data, which
also makes later evaluations on the computer much easier.
Though the reliability and robustness of Leica macroscopes is legendary,
like any high-tech product, the Leica Z series requires a certain degree of
care and attention. Therefore, we recommend that you read this manual.
It contains all the information you need regarding operation, safety and
maintenance. Simply observing a few guidelines will ensure that even
after years of intensive use, your Leica macroscope will continue to work
as smoothly and reliably as on the very first day.
We wish you the best of success in your work — after all, you are now
equipped with the best tool!
Leica Z6 APO A / Z16 APO A User Manual 13
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The Modular Design: Everything is Relative
The Leica Z series provides maximum flexibility
in choosing equipment, thanks primarily to the
modular configuration and the compatibility
that Leica has painstakingly maintained for
decades. The optics carriers, eyepieces, bases
and more can be combined in any way you
choose, allowing you to create the macroscope
that best suits your needs.
Despite this, you will notice that the controls
and individual components do not differ significantly. Whichever configuration you choose,
you will quickly feel right at home.
Have a special request? Let us know!
Leica Microsystems enjoys an exceptional reputation when it comes to devising customerspecific solutions. If you have a special request
that cannot be met with standard parts, contact
your Leica consultant. He or she has a solution
for every problem.
Leica Z6 APO A / Z16 APO A User Manual 14
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The Automatic Control System
Reliability for your experiments
For the Leica Z6 APO A and Z16 APO A automated macroscopes, motorized control of the
zoom, iris diaphragm and fine focus is possible.
This guarantees not only convenient work, but
also added reliability for your experiments, as
results can be reproduced exactly. In conjunction with the Leica LED3000 NVI™ system illumi-
The Leica SmartTouch controls all motorized
functions of the Z series and the connected
accessories.
nators, a wide variety of scenarios can be set up,
saved and restored at the touch of a button.
Control à la carte
You alone determine the degree of automation.
The zoom, focus and illumination can be optionally controlled using the "Leica Application
Suite" (LAS) software solution or via the Leica
SmartTouch touchscreen panel.
The Leica Application Suite (LAS) controls and
photographs fully automatically as desired.
To automate your workflows even more, also
integrate the Leica IsoPro™ motorized XY stage
– it positions multiple specimens fully automa-
*
tically
or turns a series of individual images
into one large, seamless illustration
**
, to give
only two examples.
Talk to your Leica partner about the wide range
of automation possibilities – he or she will show
you how to get the maximum out of your Leica
macroscope.
* With LAS auxiliary module LAS MultiStep
** With LAS auxiliary module LAS PowerMosaic
Leica Z6 APO A / Z16 APO A User Manual 15
Page 16
On We Go
If your new Leica macroscope has already been
assembled and commissioned by your Leica
consultant, click here to skip through the installation instructions and go directly to the Quick
Start Guide on page 45.
If, on the other hand, you are assembling the
Leica macroscope yourself, continue with the
"Assembly" chapter, which begins on Page 17.
Leica Z6 APO A / Z16 APO A User Manual 16
Page 17
Assembly
Leica Z6 APO A / Z16 APO A User Manual 17
Page 18
Incident-light Bases and Manual Focusing Column
The first step is to connect the focusing column
to the corresponding base.
Tools used
★ Allen key provided
Assembling the focusing column
1. Lay the focusing column on the edge of a
table or another stable substrate so that
the three threads at the end of the column
protrude over the edge.
2. Hold the incident-light to the focusing
column and secure it to the base using the
three screws provided.
Tip: The base can be installed more easily
if someone helps you do so.
Leica Z6 APO A / Z16 APO A User Manual 18
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Transmitted-light Bases and Manual Focusing Column
For additional information on the TL
bases, refer to page 29.
The first step is to connect the focusing column
to the corresponding base.
Tools used
★ Allen key provided
Assembling the column adapter
1. Securely install the column adapter on the
column using the four included screws.
Assembling the focusing drive
2. Securely screw the focusing column to the
base using the six included screws.
Leica Z6 APO A / Z16 APO A User Manual 19
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Microscope Carrier
Tools used
★ Allen key provided
Assembling the Microscope Carrier
1. Press the microscope carrier against the
focusing column.
2. Tighten the screw using the Allen key provided.
Leica Z6 APO A / Z16 APO A User Manual 20
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Video/Phototube A
The video/phototube A is used if only a
Leica digital camera with the Leica LAS
(Leica Application Suite) software is used to
observe the specimen.
Assembling the tube
1. Unscrew the locking screw on the optics
carrier.
2. Insert the tube into the microscope carrier.
3. Tighten the locking screw on the microscope carrier.
Installing the objective/camera
1. Screw the desired digital camera to the
video objective.
2. Insert the video objective into the video/
phototube A and turn the knurled ring until
tight.
Leica Z6 APO A / Z16 APO A User Manual 21
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Video/Phototube AS ("Slim")
The video/phototube AS is intended for
OEM applications and is usually not used
on Leica focusing columns.
After installation on the OEM equipment,
the rest of the components are installed
as described on the following pages.
Leica Z6 APO A / Z16 APO A User Manual 22
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Video/Phototube Y
You can use the Y-tube to attach binocular tubes, ErgoModules®, video/phototu-
bes and the Leica IC A video module of the Leica
M series.
Assembling the tube
1. Unscrew the locking screw on the optics
carrier.
2. Insert the tube into the microscope carrier
so that the locking screw faces the rear.
3. Tighten the locking screw on the microscope carrier.
Assembling the tube
1. Insert the desired tube and turn the knurled
ring until tight (refer also to page 27).
Leica Z6 APO A / Z16 APO A User Manual 23
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Coaxial Illuminator (Optional)
Tools used
★ Allen key provided
The coaxial illuminator is fitted between
the optics carrier and the tube. It enables
you to connect the light guide, which, in turn,
is connected to a cold light source of the Leica
CLS series.
At low magnifications, using the coaxial
illuminator causes vignetting (shading),
which is more or less pronounced depending
on the configuration. This vignetting is not a
malfunction.
The quarter-wave plate for coaxial illumination must be installed on the planapo-
chromatic objectives.
Installing the coaxial illuminator
1. Guide the coaxial illuminator to the tube
from the left or right.
2. Adjust the coax illuminator so that the port
for the light guide faces the direction that is
the most comfortable for your work.
3. Push the coaxial illuminator upwards and
tighten the screw on the coaxial illuminator.
4. Connect the light guide of the Leica cold
light source to the coaxial illuminator.
The User Manual for the Leica cold light
source is provided with the cold light
source.
Leica Z6 APO A / Z16 APO A User Manual 24
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Optics Carrier
Tools used
★ Allen key provided
The instructions on this page also apply
logically if the optional coaxial illumi-
nator has been installed (see page 24). In this
case, the optics carrier is attached to the coaxial
illuminator instead of the tube.Assembling the
optics carrier
1. Guide the optics carrier over the tube from
the rear.
2. Push the optics carrier upwards and tighten
the fastening screw.
Leica Z6 APO A / Z16 APO A User Manual 25
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Objective
Tools used
You can attach the quarter-wave plate
for coaxial illumination, the analyzer for
polarization or a ring illuminator to the planapochromatic objectives.
HR and DM objectives are high-performance objectives and always cause
vignetting. This vignetting is not a malfunction.
Attaching the objective
1. Screw the planapochromatic objective to
the diaphragm ring or the optional fine
focus in a counterclockwise direction.
Leica Z6 APO A / Z16 APO A User Manual 26
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Ergo binocular tube
Tools used
★ No tools required.
Prerequisite for the installation
When using the ergo binocular tube or
another tube, the Y video/phototube
must be used.
Preparations
1. Unscrew the positioning screw and remove
the protective cover.Assembling the tube
Assembling
1. Push the tube into the dovetail ring and
rotate it slightly in both directions until the
positioning screw meshes with the guide
groove.
2. While holding the tube only slightly, carefully tighten the positioning screw. It is
automatically brought to the correct position.
Leica Z6 APO A / Z16 APO A User Manual 27
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Eyepieces
Tools used
★ None
Magnification range
You can extend the overall magnification
range using available 10×, 16×, 25× and
40× wide-field eyepieces for persons wearing
glasses.
Preparation
1. If you want to use an optional graticule,
insert it now (Page 52).
2. Remove the plastic tube guard.
Inserting the eyepieces
3. Push the eyepieces into the tubes as far as
they will go and check to ensure that they
fit tightly and accurately.
4. Securely tighten the clamping screws.
Leica Z6 APO A / Z16 APO A User Manual 28
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Transmitted-light Base TL ST
Unpacking the base
The base is delivered with the adapter plate
installed. Make sure the instruments are unpacked on a flat, sufficiently dimensioned, and
non-slip surface.
Focusing drive and column
1. Unscrew the extension plate from the base
using the Allen key provided.
2. Attach your focusing drive column from
below using the four hexagon-head screws.
3. Re-attach the adapter plate to its original position using the six hexagon-head
screws.
Leica Z6 APO A / Z16 APO A User Manual 29
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Transmitted-light Base TL BFDF: Before First Use
Removing the transport anchors
Before you can use the transmitted-light
base for the first time, it is absolutely
necessary to remove the two transport anchors.
Anchor of mirror
Anchor of switching slide
Leica Z6 APO A / Z16 APO A User Manual 30
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Transmitted-light Base TL BFDF
Standard delivery
The base is delivered with the adapter plate
installed. The selected stage (Leica IsoPro™
mechanical stage or Leica standard stage
10447269), and the focusing drive will have to
be mounted later.
Stage assembly
The Leica TL BFDF transmitted-light base can be
equipped with three different stages.
★ Leica IsoPro™ manual mechanical stage
★ Leica IsoPro™ automatic mechanical stage
★ Leica standard stage 10 447 269
The selected stage is mounted on the base
before commissioning. You can switch between
the stages at any time with just a few hand
movements.
The following paragraph assumes use of the
base without the stage mounted. Disassembly
is performed in reverse order of the following
steps.
Standard stage
1. Take the glass plate from the rectangular
gap in the standard stage.
2. Position the stage on the transmitted-light
base in such way that the four holes align
over those in the base.
3. Attach the stage to the base with the four
supplied hexagon-head screws.
4. Insert the glass plate back into the standard
stage.
Leica Z6 APO A / Z16 APO A User Manual 31
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TL RC™ / TL RCI™
The base is delivered with the adapter plate
installed. The selected stage (Leica IsoPro™
mechanical stage or Leica standard stage
10 447 269), and the focusing drive will have to
be mounted later.
Ensure that the instruments are unpacked
on a level, adequately sized, and nonskid underlay.
Stage assembly
The Leica TL RC™/ RCI™ transmitted-light base
can be equipped with three different Leica
stages. The selected stage is mounted on the
base before commissioning. You can switch
between the stages at any time with just a few
hand movements.
The following paragraph assumes use of the
base without the stage mounted. Disassembly
is performed in reverse order of the following
steps.
Standard stage
1. Take the glass plate from the rectangular
gap in the standard stage.
2. Position the stage on the transmitted-light
base in such way that the four holes align
over those in the base.
3. Attach the stage to the base with the four
supplied hexagon-head screws.
4. Insert the glass plate back into the standard
stage.
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IsoPro™ Manual Mechanical Stage: Assembly
Leica IsoPro™ Mechanical Stage
Before the Leica IsoPro™ mechanical stage is
mounted to the base, the axis containing the
control buttons is attached either on the left or
the right side of the mechanical stage.
If the controls are to be mounted on the lefthand side, the gear rod on the bottom side of
the mechanical stage must be unscrewed and
reattached in reverse.
1. Take the glass plate from the mechanical
stage.
2. Turn the mechanical stage around and
place it onto a non-slip surface.
3. Change the gear rod from the left to the
right-hand side.
4. Skip the next two steps to install the controls.
Before the Leica IsoPro™ mechanical stage is
mounted to the base, the axis containing the
control buttons is attached either on the left or
the right side of the mechanical stage.
Leica Z6 APO A / Z16 APO A User Manual 33
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Leica IsoPro™ Manual Mechanical Stage: Assembly (cont'd.)
Left or right operation
If the controls are to be mounted on the lefthand side, the gear rod on the bottom side of
the mechanical stage must be unscrewed and
reattached in reverse.
1. Take the glass plate from the mechanical
stage and turn it around.
2. Change the gear rod from the left to the
right-hand side.
Control assembly
1. Take the glass plate from the mechanical
stage and turn it around.
2. Attach the axis with the control buttons to
the desired side. The fastener snaps into the
mechanical stage magnetically.
3. Attach the axis with the two supplied hexagon-head screws.
4. Attach the cover rail to the mechanical
stage.
Mechanical stage assembly
1. Place the mechanical stage onto the base.
2. Pull the upper part of the mechanical stage
carefully towards the user, fixing the lower
part onto the transmitted-light base.
3. Attach the mechanical stage evenly to the
three threaded holes.
4. Now move the mechanical stage as far as it
will go in the direction of the column.
5. Insert the glass plate into the mechanical
stage.
Leica Z6 APO A / Z16 APO A User Manual 34
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Leica IsoPro™ Manual Mechanical Stage: Assembly (cont'd.)
Focusing drive and column
1. Unscrew the extension plate from the base
using the Allen key provided.
2. Attach your focusing drive column from
below using the three hexagon-head
screws.
3. Re-attach the adapter plate to its original
position using the three hexagon-head
screws.
Leica Z6 APO A / Z16 APO A User Manual 35
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Leica IsoPro™ Motorized Mechanical Stage: Assembly
Basics
The transmitted-light bases of the Leica TL series
(TL BFDF, TL RC™, TL RCI™) are supplied with an
installed extension plate. The selected stage
(Leica IsoPro™ mechanical stage or standard stage
10 447 269) and the focusing drive will have to
be mounted later.
The motorized mechanical stage is a sensitive
precision instrument. During installation, avoid
subjecting the stage to impact or severe vibrations.
1. Unpack the mechanical stage from the
transport packaging and position it on the
transmitted-light base.
1
2
1 Microscope base
2 Motorized mechanical stage
2. Secure the motorized mechanical stage to
the base using three M4 screws.
3× M4 screws
Leica Z6 APO A / Z16 APO A User Manual 36
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Leica IsoPro™ Motorized Mechanical Stage: Assembly (cont'd.)
3. Remove the two M3 screws and
the holder from the mechanical stage.
4. Remove the two M4 screws and the sleeve
from the mechanical stage.
5. Remove the four shock-absorbing cartons
from the mechanical stage.
After removal, keep all the transport
anchors in the plastic bag provided for
future transport.
Leica Z6 APO A / Z16 APO A User Manual 37
Page 38
Leica IsoPro™ Motorized Mechanical Stage: Assembly (cont'd.)
The bases
The TL BFDF, TL RC™ and RCI™ transmitted-light
bases can be equipped with three different
stages: Standard stage, manual and automated
IsoPro™ mechanical stage. The selected stage is
mounted on the base before commissioning.
You can switch between the stages at any time
with just a few hand movements.
The following paragraph assumes use of the
base without the stage mounted. Disassembly
is performed in reverse order of the following
steps.
Mechanical stage and base
Never move the sledge of the motorized
mechanical stage manually in the X
direction, as otherwise the mechanical system
will be damaged!
1. Place the mechanical stage onto the base.
2. Pull the upper part of the mechanical stage
carefully towards the user and fasten the
lower part onto the transmitted-light base.
3. Attach the mechanical stage evenly to the
three threaded holes.
4. Now move the mechanical stage as far as it
will go in the direction of the column.
5. Insert the glass plate into the mechanical
stage.
Leica Z6 APO A / Z16 APO A User Manual 38
Page 39
Leica IsoPro™ Motorized Mechanical Stage: Assembly (cont'd.)
Mechanical stage for X-Y stage control
module
1. Plug the CTL2 plug of the mechanical stage
motor into one of the three available CTL2
interfaces.
2. Plug the CTL2 plug of the Leica PSC controller into another CTL2 interface.
★ The third CTL2 interface is available for
connecting other instruments, such as the
Leica foot switch (10 447 398) or TL RCI™
transmitted-light base (10 446 352).
★ The 15-pin Sub-D interface is intended for
use with the Leica SmartMove™ controller
(11 501 197).
3. Plug a power cable into the socket provided
and into a grounded power socket.
As soon as all instruments have been plugged
into the control box and it has been plugged
into the power supply, the mechanical stage
initializes and automatically drives to the mid
position.
4. Connect the PC (where applicable) and X/Y
Stage DCI Module (using a suitable USB
cable).
Leica Z6 APO A / Z16 APO A User Manual 39
Page 40
Cables: Connections
The A version of the Leica Z series features
extensive automation options with which various microscope data and settings can be read
out, transferred to the PC and reproduced later.
The terminals The connection to the PC and to other instru-
ments is made using the terminals on the rear
side of the column:
1
3
2
1. 2× CTL2 terminals for auxiliary equipment
such as the distribution box of the Z series,
the SmartTouch panel, the Leica TL RCI
base and other accessories from the Leica
product range.
2. Terminal for the power supply provided.
3. USB terminal for the connection to the PC.
Leica Z6 APO A / Z16 APO A User Manual 40
Page 41
Cables: Cable Duct
The integrated cable duct in the column enables a neat cable layout around the macroscope.
For example, the USB or FireWire cables of the
camera can be stowed in the cable duct.
Feeding the cables
1. Unscrew the three screws on the cable
duct.
2. Remove the cover of the cable duct.
3. Place the cables in the cable duct and screw
the cover on tightly.
Tip: Estimate the length of the cable ends you
will need before screwing on the cover. For
thick cables, it is difficult to change the length
retroactively.
Leica Z6 APO A / Z16 APO A User Manual 41
Page 42
Motorized Focus: Adjusting the Travel Path
Depending on the work situation, it is
necessary to restrict the maximum travel
path of the macroscope. This prevents the
following:
★ Injuries when manipulating the specimen
because the fingers or hand get pinched.
★ Accidental contact between the objective
and the specimen, and potential resulting
damage.
Readjusting the motorized focus
The motorized focus is factory-adjusted and
normally does not need to be readjusted—
even if the maximum travel path is changed.
Exception: If the power fails while the
motorized focus is moving, the position
data are lost. In this case, the calibration must
be repeated using the Leica LAS software or the
Leica SmartTouch™. To do so, please consult the
respective manual.
Restricting the bottom travel range
1. Move the motorized focus into the lowest
position you want to reach.
2. Unscrew the screw of the limit stop on the
side of the focusing column.
3. Push the limit stop to the height of the
motorized focus.
It is easiest to move the limit stop by keeping the
screwdriver inserted and moving it upwards.
4. Tighten the screw of the limit stop.
Leica Z6 APO A / Z16 APO A User Manual 42
Page 43
Cables: Diagram
1/3
Z6 / Z16, APO & APO A
with Z-Series DCI-Box
PC
CTL2
CTL2
Power plug
CTL2-Ports
Z6 / Z16 APO A
™
Powersupply
10 450 266
* Serial connection loop possible
** Adapter-cable 10 447 401 needed
*** Serial connection of up to 5 footswitches possible
Leica Z6 APO A / Z16 APO A User Manual 43
SmartTouch
* Footswitch***
CTL1
Z-Series
DCI -Box
Connection between
serial- and CTL1-Port **
UMC
Page 44
Cables: Diagram
PC
2/3
Interface on Focus
Column
Z6 / Z16, APO & APO A
with interface on HighPerformance-M-Series-Column
Z6 / Z16 APO A
Z-Series
DCI-Box
CTL2
CTL2
CTL1
™
TL RCI
USB
* Serial connection loop possible
** Serial connection of up to 5 footswitches possible
*** For motorfocus only
**** Not with new motorfocus
SmartTouch™ * Footswitch** Handwheel***
Leica Z6 APO A / Z16 APO A User Manual 44
CTL2-Ports
Power Supply
10 450 298
Do not use CTL1-Port and
Power Plug when DCI-Box
is connected to the
motor focus column!
UMC****
Page 45
Cables: Diagram 3/3
PC
Interface on Focus
Column
CTL2
USB
USB
CTL2
Z-Series
DCI-Box
Power Supply
CTL2
CTL2
CTL1
Z6 / Z16, APO & APO A
with TL RCI™ Base and IsoPro™ Stage
Do not use CTL1-Port and
Power Plug when DCI-Box
is connected to the
motor focus column!
DCI Controlbox
TL RCI
Power
™
CTL2 CTL2 CTL2
Z6 / Z16 APO A
Alternative installation
Please use the Leica Application Suite
to configure the functions of the system.
LED3000 NVI
Leica Z6 APO A / Z16 APO A User Manual 45
SmartTouch
™
CTL2 CTL2
Smart Move Connector from
PSC
IsoPro™ Stage
Page 46
Leica LED3000 NVI™ (Near Vertical Illumination)
Required tools
★ None
The Leica LED 3000 NVI™ can be used
with any objectives that have an outer
diameter of 58 mm.
The supported working distance is
between 50 mm and 150 mm.
Installing the Leica LED3000 NVI™
1. Connect the Leica LED3000 NVI™ to the
focusing column via the CTL2 connection if
the focusing column is equipped with integrated electronics.
Alternative installation for manual columns
1. Connect the external power supply unit (10
450 266) to the Leica LED3000 NVI™.
2. Place the Leica LED3000 NVI™ on the objective from below and tighten the locking
screw.
Leica Z6 APO A / Z16 APO A User Manual 46
Page 47
Quick Start Guide
Leica Z6 APO A / Z16 APO A User Manual 47
Page 48
Overview of an automated Leica Z Series Macroscope (Leica Z16 APO A)
1 Digital camera
2 Photo/videotube
3 Eyepieces
4 Video/Phototube Y
5 Microscope carrier
6 Connection for control cable
7 Optics carrier (zoom body)
8 Objective
9 LED ring illuminator
10 IsoPro™ motorized mechanical
stage
11 SmartTouch control unit
12 Motorized microscope column
10
1
2
3
4
5
6
7
8
9
12
11
Leica Z6 APO A / Z16 APO A User Manual 48
Page 49
Eyepieces
Leica Z6 APO A / Z16 APO A User Manual 49
Page 50
Magnification Factors of the Eyepieces
An eyepiece not only makes it possible to look passively into the macroscope, but also has a critical effect on the maximum magnification. The
magnification factor is between 10× and 40×.
The following eyepieces are available for the Z series:
Magnification Dioptric Correction Order number
10× ± 5 diopter settings 10 450 023
16× ± 5 diopter settings 10 450 024
25× ± 5 diopter settings 10 450 025
40× ± 5 diopter settings 10 450 026
Leica Z6 APO A / Z16 APO A User Manual 50
Page 51
Using the Eyepieces
The eyepieces form the connection between
the tube and the eye of the observer. Simply
push them into the tube and they are ready to
use.
Each eyepiece offers a certain magnification
factor that has a determinative effect on the
total magnification. Furthermore, all Leica
eyepieces can be equipped with practical graticules that enable measuring and quantifying of
specimens.
Dioptric Correction
A built-in dioptric correction is available for
eyeglass wearers. For more information, refer
to Page 51.
If you do not wear glasses:
1. Hold the eyepiece firmly and rotate the
eyecups forwards counterclockwise.
2. If an eyepiece is equipped with the integrated dioptric correction, turn the value to
the "0" mark.
If you wear glasses:
1. Hold the eyepiece firmly and rotate the
eyecups clockwise towards the rear, as
otherwise the viewing distance is too great.
2. If an eyepiece is equipped with the integrated dioptric correction, turn the value to
the "0" mark.
By the way, one benefit of viewing with eyeglasses is a drastically lower risk of bacterial transmission (see page 53). The soft material of the
eyecup also ensures that your glasses will not
be scratched, even if they contact the eyepiece.
Leica Z6 APO A / Z16 APO A User Manual 51
Page 52
The Correct Interpupillary Distance
The interpupillary distance is correctly set if you
see a single circular image field when looking
at a specimen.
If you are still a novice macroscope user, you
may need a short time to become accustomed
to this. Not to worry — after a little while, it will
become automatic.
Reference value
The distance between eye and eyepiece measures approx. 22 mm for 10/23B wide-field eyepieces for persons wearing glasses.
Adjusting the interpupillary distance
1. Look into the eyepieces.
2. Hold the eyepieces with both hands. Push
the eyepieces together or separate them
until you see a circular image.
3. Slowly approach the eyepieces with your
eyes until you can see the complete image
field without corner cutting.
✗
✓
Leica Z6 APO A / Z16 APO A User Manual 52
Page 53
Dioptric Correction
Leica macroscopes are parfocally matched. A
prerequisite for this the correct setting of the
diopters. The following adjustments only have
to be carried out once by each user.
Therefore, all Leica eyepieces are also available
with built-in dioptric correction, allowing the
macroscope to be used without glasses even
by those with vision problems. The correction
comprises ±5 diopter settings.
Using the Dioptric Correction
1. Set the dioptric correction of both eyepieces to the mid position ("0" diopter
settings).
2. Look through the eyepieces and focus on a
flat specimen.
3. Rotate both eyepieces to the maximum
value of "+5".
4. Hold one eye closed and rotate the other
eyepiece in the "-" direction until the specimen appears sharp.
5. Then, open the other eye and correct the
diopter settings until the image is uniformly
sharp.
6. Select the highest magnification and refocus if necessary.
Now, if you adjust the magnification from the
lowest to the highest level, the specimen is
always brought into sharp focus. If not, repeat
the process.
The system is now parfocally matched to your
eyes.
Leica Z6 APO A / Z16 APO A User Manual 53
Page 54
Graticules
Leica graticules make length measurements and counting easier, particularly
for workstations that are not equipped with a
digital camera and LAS software.
Use
The Leica graticules for length measurements
and numbering are fitted in mounts and are
inserted into the eyepieces.
1. Screw the insert off of the eyepiece.
2. Clamp the graticule on the insert, applying
moderate pressure. Ensure that the graticule fits tightly.
3. Screw the insert and graticule firmly into
place and replace the eyepiece in the tube.
4. You can now align the graticule by rotating
the eyepiece in the tube and then tightening it using the clamping screw.
Leica Z6 APO A / Z16 APO A User Manual 54
Page 55
Health Notes
Potential sources of infection
Direct contact with eyepieces is a potential transmission method for bacterial
and viral infections of the eye. The risk can be
kept to a minimum by using individual eyepieces or detachable eyecups. Eyecups can be
ordered separately. Please contact your Leica
partner.
Separate eyecups are an effective way
of preventing infections.
Leica Z6 APO A / Z16 APO A User Manual 55
Page 56
Photography & Video
Leica Z6 APO A / Z16 APO A User Manual 56
Page 57
Photography & Video
For most macroscope users, digital documentation has become an invaluable part of their
work. Research results can be presented in an
attractive manner; measurements on the digital image provide clarity and, in conjunction
with the motorized IsoPro™ mechanical stage,
even images of large specimens can be captured step by step and automatically joined to
create a new complete image.
Adapters
If camera control using the Leica Application
Suite is not required, conventional mirror reflex
and rangefinder cameras from third-party
manufacturers can be used. For this purpose,
Leica Microsystems offers a variety of adapters
that can be used together with the 50% and
100% trinocular tubes.
Leica DFC cameras
However, if you require absolute control over
the camera and need the capability for measurement, evaluation and more in addition to
photography, the digital Leica DFC cameras are
exactly right for you. Together with the Leica
Application Suite, they provide virtually limitless freedom of use. For additional information about Leica cameras, refer to the camera's
documentation.
Leica Application Suite
The "Leica Application Suite", or "LAS" for short,
is, as it were, the digital extension of the Leica
Z series macroscopes. In addition to capturing
images, it lets you control the macroscope, illuminator, stages, cameras and more. For additional information, refer to the LAS online help.
Leica Z6 APO A / Z16 APO A User Manual 57
Page 58
Photo Tubes and C-mounts
Application
All Leica DFC cameras are equipped with a
standardized C-mount interface. In turn, the
C-mount adapter for the respective trinocular
tube is connected to this interface. This adapter
creates a solid mechanical connection between
the macroscope and camera and ensures optimum rendering of the macroscopic image on
the image sensor of the camera.
Usually, the ideal is for the digital camera to
capture as much of the field of vision as possible, while excluding as much of the black edge
of the field of vision as possible. To do so, the
magnification factor of the C-mount adapter
must match the image format of the sensor as
closely as possible (see table).
If there is unwanted shading at the corners even
with a compatible C-mount adapter installed, it
can be corrected using the "Shading function"
of the camera software.
Alternatively, you can also use a C-mount adapter with higher magnification. This primarily
avoids the critical border area of the field of
vision and concentrates on the center of the
field of vision.
Camera optimal
(large
image field)
DFC295 0.5× 0.63×
DFC420 0.5× 0.63×
DFC490 0.63× 0.8×
DFC500 0.63× 0.8×
suitable
(smaller
image field)
Cameras from third-party suppliers
In addition to Leica DFC cameras with the standardized C-mount interfaces, you can connect
third-party cameras such as single-lens reflex
cameras to the macroscope using a T2 bayonet
adapter. To do so, instead of the C-mount adapter, simply use the corresponding SLR adapter
with T2 connection. However, these third-party
cameras are not integrated into the Leica Application Suite and have to be operated using
the corresponding software from the camera
manufacturer.
The Leica digital cameras are detailed in a separate user manual along with instructions for
their assembly and use.
Leica Z6 APO A / Z16 APO A User Manual 58
Page 59
Trinocular Video/Phototube 50%
Use
With its third beam path, the trinocular video/
phototube 50% enables you to simultaneously
view and photograph a specimen. The available
light is divided as follows:
★ 50% is available for the two eyepieces.
★ 50% of the light is diverted to the video/
photo beam path.
Assembly
Fasten the "trinocular tube50%" to the optics
carrier instead of the ErgoTube
page 27).
™
(refer also to
Leica Z6 APO A / Z16 APO A User Manual 59
Page 60
Trinocular Video/Phototube 100%
Use
With its third beam path, the trinocular video/
phototube 100% enables you to either view or
photograph a specimen. This means that 100%
of the light is available to one or the other beam
path. The other beam path remains opaque or
black.
Assembly
Fasten the "trinocular tube100%" to the optics
carrier instead of the binocular ErgoTube (refer
also to page 27).
Switchover
★ Turn the controller on the right side of the
tube into the horizontal position in order to
guide all available light into the eyepieces.
You can now observe the specimen.
★ Turn the controller on the right side of the
tube into the vertical position in order to
guide all available light into the camera.
You can now photograph the specimen.
Leica Z6 APO A / Z16 APO A User Manual 60
Page 61
Objectives and
Optical Accessories
Leica Z6 APO A / Z16 APO A User Manual 61
Page 62
The Different Types of Objectives
To meet the various requirements regarding
imaging properties, there is a choice of highquality interchangeable planachromatic and
planapochromatic objectives and also lowerpriced interchangeable achromatic objectives.
You can use the objectives on the Z6 APO or Z16
APO zoom systems or with the fine focusing.
★ We recommend using the 1×, 2×, 5×, 0.8×,
0.5× planapochromatic objectives in order
to take advantage of the high performance
of the apochromatic zoom system.
★ You can attach the quarter-wave plate for
coaxial illumination, the analyzer for polarization or a ring illuminator to the planapochromatic objectives.
★ Also available are the M series 0.63×, 0.5×,
0.32× achromatic objectives, which can be
attached to the zoom or the fine focusing
using an adapter.You can attach the vertical
illuminator or the attachment for incident/
oblique observation to the achromatic
objective.
Achromatic objectives with a long focal
length
For special applications, achromatic objectives
with long working distances and focal lengths
of f=100 mm to 400 mm are available.
Leica Z6 APO A / Z16 APO A User Manual 62
Page 63
Bases
Leica Z6 APO A / Z16 APO A User Manual 63
Page 64
Leica TL ST Transmitted-light Base: Controls
1
2
3
4
1 Adapter plate for easy assembly of focusing
drives
2 Removable glass plate
3 Controller for light intensity
4 Adjustment for deflection mirror
Extension plate of the
transmitted-light base TL ST
1 2 3
Rear side of the transmitted-light base TL ST
1 Screws for changing the halogen lamp
2 Power connection socket
3 Power switch
Leica Z6 APO A / Z16 APO A User Manual 64
Page 65
Leica TL ST Transmitted-light Base: Operation
Light intensity control
The left control adjusts the intensity of the
12 V/20 W halogen illumination.
1. Switch on the illumination of the base at
the power switch.
2. Focus on the specimen.
3. Set the illumination to the desired intensity
using the left control.
Transmitted-light control
The transmitted-light base TL BFDF has a slider
that automatically moves the deflection mirror
in the base when moved. The mirror is kept in
the correct position at all times and permits
smooth changeover between bright field and
opaque transmitted light.
Bright field
Bright field is suitable for examining translucent objects featuring contrasting structures.
The object is directly illuminated from below
and is seen in its natural colors against a bright
background.
★ Move the slider backwards until the desired
effect is achieved.
Inclined transmitted light
Transmitted light that traverses the object obliquely will provide additional resolution and
information when observing semitransparent,
opaque objects.
★ Slowly pull the slider towards yourself until
the desired effect is achieved.
Leica Z6 APO A / Z16 APO A User Manual 65
Page 66
Leica TL ST Transmitted-light Base: Changing Bulbs
Changing the halogen lamp
Before you change the lamp, it is absolutely necessary to unplug the power
plug from the base to prevent the risk of electric shock!
The halogen lamp becomes very hot
during operation. Therefore, to avoid
being burned, let the base cool off for approx.
10 minutes after switching it off!
Do not touch new halogen lamps with
your bare fingers—this drastically redu-
ces the service life of the lamp!
Changing Bulbs
1. Unscrew the two screws on the heat sink
and pull the heat sink out, along with the
lamp.
2. Carefully pull out the lamp and mount by
pulling them upwards.
3. Disconnect the lamp from the mount.
4. Insert the new lamp into the mount and
reinsert the lamp holder.
Precautionary measures
When inserting the lamp, ensure that
the cables are inside the two metal
clamps. This prevents the cables from getting
caught during insertion.
Leica Z6 APO A / Z16 APO A User Manual 66
Page 67
Leica TL BFDF Transmitted-light Base: Controls
1
2
3
1 Adapter plate for easy assembly of focusing
drives
2 Standard stage 10 447 269
3 Button to toggle between bright field and
dark field
Extension plate of the
transmitted-light Base TL BFDFb
Adapter at the focusing drive
Button to toggle between bright field and dark
field
Leica Z6 APO A / Z16 APO A User Manual 67
Connector for cold light sources
(light conductor active f = 10mm,
end tube f = 13mm)
Page 68
Leica TL BFDF Transmitted-light Base: Operation
Light intensity control
Observe the user manual—in particular, all safety regulations—from the
manufacturers of the light guide and cold light
source.
★ Switch on the cold light source according to
the manufacturer's user manual and adjust
the brightness.
Transmitted-light control
The Leica TL BFDF transmitted-light base has
a control that switches the light from "bright
field" to "dark field".
Bright field
Bright field is suitable for examining translucent objects featuring contrasting structures.
The object is directly illuminated from below
and is seen in its natural colors against a bright
background.
★ Turn the control as far as it will go towards
"BF" ("bright field").
Fingertip with bright field illumination Identical subject with dark field illumination
Dark field
In dark-field illumination, a ring illuminator is
used in such a way that the direct light
does not reach the objective without a specimen. Only the structure of semitransparent,
opaque objects disperses the light, making the
object visible against a dark background.
★ Turn the control as far as it will go towards
"DF" ("dark field").
Leica Z6 APO A / Z16 APO A User Manual 68
Page 69
Leica TL RC™ / TL RCI™: Controls
1
2
3
4
5
6
7
1 Heat sink of the integrated halogen
illumination (only TL RCI™)
2 Extension plate for focusing drives
3 Standard stage 10 447 269
4 Filter holder
5 Control of top and bottom flaps of the
Rottermann Contrast™
6 Button for mirror and horizontal movement
of the mirror
7 Transmitted-light base
Vertical column —
transmitted-light base adapter plate
1
2
3 4 5 6
1 Power switch
2 Power connection socket
3 USB socket, type B
4 USB socket, type A
5 2 CAN bus
6 Screws for changing the halogen lamp
Leica Z6 APO A / Z16 APO A User Manual 69
Page 70
Leica TL RCI™: The Deflection Mirror
1
Inverted world?
Depending on the properties of the specimen
(refractive index of the environment) and the
perception of the viewer, it is possible that the
switches described below for positive and inverted relief contrast are to be operated in reverse.
This means that the lower switch, rather than
the upper switch, controls the inverted relief
contrast and vice-versa.
Tilted mirror
The built-in mirror features one flat and one
concave side and can be rotated and moved.
The concave side has been specially designed
for the optical requirements of objectives with
a high numerical aperture. The black rotary
knob on the left side of the transmitted-light
base can be used to rotate the built-in pathfolding mirror and move it forwards/back.
The concave cutout on the handle indicates the
concave side of the mirror, allowing intuitive
operation at any time without eye contact.
The angle of light incidence in the specimen
plane changes depending on the tilt and
position of the mirror. As a result, switching
between transmitted-light bright field, oblique
illumination and dark field-like illumination is
possible.
Functions of the rotary knob
The rotary knob (1) fulfills the following tasks:
★ Turning the deflection mirror from the flat
to the concave side
★ Slight tilting to guide the light beam
through the specimen plane at a steeper or
flatter angle
★ Moving the deflection mirror (forwards/
back)
Leica Z6 APO A / Z16 APO A User Manual 70
Page 71
Leica TL RCI™: Color Intensity and Temperature
1 2
1 Potentiometer for controlling the CCIC™
(Constant Color Intensity Control)
illumination intensity
2 Potentiometer for controlling the color
temperature
The transmitted-light base TL RCI™ has two
electronic potentiometers that control the color
intensity (1) and color temperature (2).
The controller for the color temperature simultaneously serves as an electronic shutter:
★ To interrupt your work, click the potentio-
meter (2).
★ Click again to switch on the illumination.
The electronics returns the color temperature to the previously configured settings.
Using a USB mouse (only TL RCI™)
The Leica USB mouse controls the CCIC™ and
dimming function of the TL RCI™ base. Connect
the mouse to the corresponding USB port of
the base.
★ The scroll wheel of the mouse is occupied
by default with the CCIC™ control system
and is used to control the illumination
intensity.
★ To switch the illumination on or off, briefly
click the scroll wheel.
★ To enter or exit dimming mode, click and
hold down the scroll wheel for longer than
2 seconds.
Leica Z6 APO A / Z16 APO A User Manual 71
Page 72
Leica TL RC™ / TL RCI™: Operation
Setting the relief contrast
The two switches on the left side of the transmitted-light base TL RC™/TL RCI™ activate two
built-in flaps. The outer switch (1) controls the
inverted relief contrast, and the inner switch (2)
adjusts the positive relief contrast.
1 2 3
1 Switch for adjusting the inverted relief cont-
rast
2 Switch for adjusting the positive relief cont-
rast
3 Deflection mirror
Depending on the flap position, a part of
the opening of the built-in Fresnel lenses are
covered, which results in the different contrast
effects. Phase structures typically act as spatial,
relief-type images—in the positive relief contrast like hills, in the inverted relief contrast like
valleys.
Increased contrast without relief is attained if
both diaphragms are set to 45°. A gap-like illuminated area is created. By tilting the deflection
mirror slightly, you can move the gap over the
entire field of view and quickly toggle between
positive and negative relief images. The dynamic effect makes it easy to distinguish phase
structures from amplitude structures.
Leica Z6 APO A / Z16 APO A User Manual 72
Page 73
Leica TL RCI™: Methods in Transmitted Light
Vertical bright-field illumination
Suitable for stained amplitude specimens with
sufficient contrast.
The light beams are deflected vertically through
the specimen. This results in an accurate bright
field with maximum brightness.
Inclined transmitted light
Suitable for semitransparent, opaque specimens such as foraminifera and fish eggs. Move
the deflection mirror until the desired data are
visible.
Single-sided dark field
Suitable for fixed specimens and fine structures.
The flatter the angle at which the light beams
are deflected into the specimen plane, the
darker the substrate appears. A dark field-like
transmitted light is created. Outlines, fine edges
and structures are bright, in contrast with the
dark background, through diffraction of the
light beams on the dark background.
Leica Z6 APO A / Z16 APO A User Manual 73
Page 74
Leica TL RCI™: Relief Images
Starting position
1. Push the deflection mirror all the way back
towards the column.
2. Turn the deflection mirror into the notch
position at an angle of 45°.
Positive relief contrast
Suitable for semitransparent and transparent
specimens. The phase structures look like hills.
The effect can be strengthened or weakened by
gently tilting the deflection mirror.
Negative relief contrast
Suitable for semitransparent and transparent
specimens. These settings result in a negative relief contrast. Phase structures look like
valleys.
The effect can be strengthened or weakened by
gently tilting the deflection mirror.
Leica Z6 APO A / Z16 APO A User Manual 74
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Leica TL RCI™: Relief Images (cont'd.)
Dynamic relief contrast
Suitable for semitransparent and transparent
specimens.
By tilting the deflection mirror slightly, you can
move the gaps over the entire field of view and
quickly toggle between positive and negative
relief images. The dynamic effect makes it easy
to distinguish phase structures from amplitude
structures.
Constraints
The relief methods provide good results from
mid-zoom to high magnifications and with 1×,
1.6× and 2× objectives. In the lower zoom half
and with weaker specimens, the object field
may not be uniformly illuminated.
We recommend using the transmitted-light
base with 1× or higher objectives, and not
objectives with a long focal length.
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Using Filters
Filters for Leica TL RC™ and TL RCI™
The transmitted-light bases TL RC™ and TL RCI™
can be equipped with up to three filters—
available as accessories—simultaneously. By
customer request, the filters are also available
as one-off items.
1. Switch off the light source or click (Leica TL
RCI™) the button for the shutter.
2. Take the empty filter from an available filter
slot in the filter holder.
3. Insert the desired filter.
4. Switch the light source back on.
Daylight filter for Leica TL ST
A daylight filter is also available for the Leica TL
ST transmitted-light base.
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Leica IsoPro™ (Non-motorized): Controls
Operating the Leica IsoPro™ mechanical
stage
1. To move the stage in the X direction, rotate
the outer knob.
2. To move the stage in the Y direction, rotate
the inner control ring.
1 2
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Leica IsoPro™ (Motorized): Controls
IsoPro X/Y Stage DCI module Leica PSC Controller
1 2
1 Leica IsoPro™ motorized mechanical stage
2 Housing with motorization
1 2 3 4
1 Sub-D interface for Leica SmartMove™
2 3 CTL2 interfaces
3 USB interface (type B)
4 Socket for grounded power cable
1
2
3
1 Quick control/memory function
2 Fine control in X direction
3 Fine control in Y direction
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System Illumination
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Leica LED3000 NVI™ (Near Vertical Illumination)
The intensity of the illuminator can be
adjusted in 10 increments.
The Leica LED3000 NVI™ can also be
controlled via the Leica Application Suite
(LAS) or the Leica SmartTouch.
The supported working distance is
between 50 mm and 150 mm.
Use
The light of the Leica LED3000 NVI™ can
be very bright. Therefore, always switch
on the illuminator before you look through the
eyepieces!
1. Switch on the illuminator by briefly pressing the
The green LED on the upper left corner now
lights up.
(ON/OFF) button.
2. Adjust the brightness by briefly pressing
the
or buttons.
3. Switch off the illuminator by briefly pressing the
button.
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Accessories
Leica Z6 APO A / Z16 APO A User Manual 81
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Leica PSC Controller
Use
The Leica PSC controller gives you precision
control of the Leica IsoPro™ motorized mechanical stage.
Connection
Connect the Leica PSC controller and the Leica
IsoPro™ motorized mechanical stage to the DCI
box.
Passing over the specimen quickly
★ Move the joystick in any desired direc-
tion (including diagonally) to control the
mechanical stage quickly.
Fine control of the mechanical stage
The motorized Leica IsoPro™ mechanical stage
offers an accuracy of up to 0.25µm. To move to
a position with maximum accuracy, use the left
and right knobs on the joystick.
Control by LAS
The instructions for controlling the Leica
IsoPro™ using the Leica Application Suite are
provided in the software's help file.
Leica Z6 APO A / Z16 APO A User Manual 82
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Leica SmartTouch
The Leica SmartTouch allows you to
control and save all automatic functions
and reload them if required. This gives you
maximum assurance for your experiments,
since you can reproduce every situation quickly
and easily.
For additional information about the
Leica SmartTouch and operation, refer to
the User Manual provided with the instrument.
Leica Z6 APO A / Z16 APO A User Manual 83
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Manual Control
Focusing
Turning counterclockwise = downwards
Turning clockwise = upwards
Toggle between
coarse and fine focusing
1. Press the black button briefly.
When the system is switched on, the
coarse focus is always switched on also.
Information about the focus positions
★ Focus positions can be stored using the
manual control or on the computer.
★ A total of up to 5 focus positions can be
stored.
★ If a sixth focus position is stored, the one
nearest to it will be deleted.
★ Each focus position can be canceled indivi-
dually.
★ The system will travel to each of the indi-
vidual focus positions in the sequence in
which they were stored.
★ Switching off the system cancels all of the
stored focus positions.
Saving the focus positions
1. Focus on the first specimen detail.
2. Press the red switch for at least 1.5 seconds.
Short beeps provide confirmation.
3. Focus on the next specimen details and
save these positions also.
Moving to stored focus positions
1. Press the red switch briefly.
Deleting stored positions
Press the red switch until you hear the audible
signal: 2 short beeps – pause – 2 long beeps.
Leica Z6 APO A / Z16 APO A User Manual 84
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Footswitch
Focusing
1 Upwards
2 Downwards
Toggling between coarse and fine focus
Press the left and right switches simultaneously.
1
2
Focus positions cannot be stored using
the footswitch.
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Dimensional Drawings
Leica Z6 APO A / Z16 APO A User Manual 86
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Leica Z6 APO A
Leica Z6 APO A / Z16 APO A User Manual 87
Page 88
Leica Z16 APO A
Leica Z6 APO A / Z16 APO A User Manual 88
Page 89
Objectives
Leica Z6 APO A / Z16 APO A User Manual 89
Page 90
Video/phototube AS
Leica Z6 APO A / Z16 APO A User Manual 90
Page 91
Y tube
Leica Z6 APO A / Z16 APO A User Manual 91
Page 92
Video/Phototube A
Leica Z6 APO A / Z16 APO A User Manual 92
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Microscope carrier
Carrier for AS tube
Leica Z6 APO A / Z16 APO A User Manual 93
Page 94
Focusing arm for probers
Ø43
0.5
37
71
69
60
117
77.5
25
Ø76
Ø87
Interface
Lowest
Focusing
Position
24
25.5
24
40
4x Ø4.5 holes (M4) for Machine Interface
54
10
Highest Focusing Position
Leica Z6 APO A / Z16 APO A User Manual 94
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Focusing arms
Highest
101
69
Focusing
Positio
Ø76
25
Lowest
Focusing
Position
Ø87
25
25
60
25
Interface for Ø25 Column
Focusing arm for bonders Tiltable focusing arm
Leica Z6 APO A / Z16 APO A User Manual 95
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Adapters
∅ 45 mm
M42
(Fine Focus Interface)
40 mm
∅
6 mm
11 mm
8 mm
M60
(Achromat Objective Interface)
66 mm
∅
Adapter for achromatic objectives Adapter for DM objectives
Leica Z6 APO A / Z16 APO A User Manual 96
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Coaxial incident-light housing
Leica Z6 APO A / Z16 APO A User Manual 97
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Specifications
Leica Z6 APO A / Z16 APO A User Manual 98
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Leica Z6 APO A & Z16 APO A
Leica Z6 APO A Leica Z16 APO A
Zoom 6.3:1, motorized 16:1, motorized
Zoom factor 0.57× – 3.6× 0.57× – 9.2×
Built-in iris diaphra gm ✔ (motorized) ✔ (motorized)
Engageable zoom posit ions
Zoom speed 1.6 s for zoom range 2.5 s for zoom range
Visual data with 1× plana pochromatic objective / 10× eyepieces / 1.25× Y tube
Magnification 7.1× – 45× 7.1× – 115×
Resolution 60 – 351 Lp / mm 51 – 336 Lp / mm
Visible structural wid th 8.3 – 1.4 µm 9.8 – 1.49 µm
Numerical Apert ure 0.02 – 0.117 nA 0.017 – 0.112 nA
Object field ∅ 29.5 mm – 4.7 mm 29.5 mm – 1.83 mm
Depth of field (diaph ragm open) 3.1 mm – 0.09 mm 3.8 mm – 0.05 mm
Depth of field (dia. closed) 18.1 mm – 0.4 mm 18.4 mm – 0.4 mm
Visual data with 2× plana pochromatic objective / 40× eyepieces / 1.25× Y tube
Magnification 57× – 360× 57× – 920×
Resolution 120 – 702 Lp / mm 102 – 672 Lp / mm
Visible structural wid th 4.2 – 0.7µm4.9 – 0.74µm
Numerical Apert ure 0.04 – 0.234 nA 0.034 – 0.224 nA
Object field ∅ 4.2 mm – 0.67 mm 4.2 mm – 0.26 mm
Data with Leica DFC49 0 digital camera / 1× planapochromatic ob jective / AS tube / 0.63× video objec tive
Magnification
Chip: Object 0.36× – 2.3× 0.36× – 5.8×
Digital resolution* 33.3 – 210 Lp / mm 33.3 – 336 Lp / mm
Object field projec ted onto chip
Depth of field (diaph ragm open) 1.06 mm – 0.03 mm 1.4 mm – 0.03 mm
Depth of field (dia. closed) 10.7 mm – 0.26 mm 10.9 mm – 0.3 mm
Continuously motorized and
approachable positions
24.5 mm × 18.4 mm /
3.9 mm × 2.9 mm
Continuously motorized and approachable positions
24.5 mm × 18.4 mm /
1.5 mm × 1.14 mm
Leica Z6 APO A / Z16 APO A User Manual 99
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Optical Accessories for Leica Z6 APO A & Z16 APO A
Leica Z6 APO A & Z16 APO A
Objectives • Planapochromat1×,2×,0.8×,0.5×,5×
• Mseriesachromaticobjectives0.63×,0.5×,0.32×,lead-free 0.5×,0.32×,lead-free
Working distances • 187mm(0.5×planapochromat)
• 97mm(1×planapochromat)
• 112mm(0.8×planapochromat)
• 39mm(2×planapochromat)
• 19mm(5.0×planapochromat)
Objective adapters • ForMseriesachromaticobjectives
• For10×and20×HRobjectives
• For10×and20×DMobjectives
DM objectives (suitable for the
upper zoom range only)
Fine Focusing 10 mm path, motorized and integrated
Binocular tubes, ergonomics • Inclinedandstraightbinoculartubes
Interpupillary distance 55 mm – 75 mm
Ergonomic wide-field eyepieces
for persons wearing glasses
Electrical interface RS232, USB via motorized focus
• DMobjectiveNPlanL20×/0.40corr
• DMobjectiveNPlan10×/0.25–/A5.8
• ApochromaticErgoTube®10º–50ºwithsynchronizedinterpupillarydistanceadjustment
• VariousErgoModules®(optional)
10× / 21, 16× / 14, 25× / 9.5, 40× / 6, distortion-free, attachable eyecups for protection from infection
ErgoTube® and ErgoModule® are registered in the United States Patent and Trademark Office
Leica Z6 APO A / Z16 APO A User Manual 100
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