Leica Z16 APO, Z6 APO User Manual
Leica Z6 APO
Leica Z16 APO
User Manual
General Instructions
Safety Concept
Before using your macroscope for the first time,
please read the "Safety Concept" brochure
included with your instrument. It contains additional information about handling and care.
Use in clean rooms
The Leica Z series can be used in clean rooms
without any problems.
Cleaning
★ Do not use any unsuitable cleaning agents,
chemicals or techniques for cleaning.
★ Never use chemicals to clean colored
surfaces or accessories with rubberized
parts. This could damage the surfaces, and
specimens could be contaminated by abraded particles.
★ In most cases, we can provide special solu-
tions on request. Some products can be
modified, and we can offer other accessories for use in clean rooms.
Servicing
★ Repairs may only be carried out by Leica
Microsystems-trained service technicians.
Only original Leica Microsystems spare
parts may be used.
Responsibilities of person in charge of
instrument
★ Ensure that the Leica macroscope is opera-
ted, maintained and repaired by authorized
and trained personnel only.
Leica Z6 APO / Z16 APO User Manual 2
Important Safety Notes
User Manual
The individual modules of the Leica Z series
include an interactive CD-ROM with all relevant
user manuals in other languages. Keep it in a
safe place, and readily accessible to the user.
User manuals and updates are also available for
you to download and print from our web site
www.leica-microsystems.com.
This User Manual describes the special functions of the individual modules of the Leica
Z series and contains important instructions
for their operational safety, maintenance, and
accessories.
The "Safety Concept" booklet contains additional safety information regarding the service
work, requirements and the handling of the
macroscope, accessories and electrical accessories as well as general safety instructions.
You can combine individual system articles
with articles from external suppliers (e.g. cold
light sources, etc.). Please read the user manual
and the safety requirements of the supplier.
Before installing, operating or using the instruments, it is mandatory to read the user manuals
listed above. In particular, please observe all
safety instructions.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
Leica Z6 APO / Z16 APO User Manual 3
Symbols Used
Warning of a danger
This symbol indicates especially important information that must be read and
•
complied with.
Failure to comply can cause the following:
★ Hazards to personnel
★ Functional disturbances or damaged inst-
ruments
Warning of hazardous electrical voltage
This symbol indicates especially important information that is mandatory to
read and observe.
Failure to comply can cause the following:
★ Hazards to personnel
★ Functional disturbances or damaged inst-
ruments
Danger due to hot surface
This symbol warns against touching
accessible hot surfaces, e.g. those of
light bulbs.
Important information
This symbol indicates additional information or explanations that intend to
provide clarity.
Explanatory notes
★ This symbol within the text stands for addi-
tional information and explanations.
Figures
(1) Numbers in parentheses within the
descriptions relate to the figures and the
items within those figures.
Leica Z6 APO / Z16 APO User Manual 4
Safety Instructions
Description
★ The individual modules fulfill the highest
requirements for observation and documentation with the Leica Z series.
Intended use
★ Refer to "Safety Concept" booklet.
Non-intended use
★ Refer to "Safety Concept" booklet.
Never use Z series macroscopes or their components for surgical procedures (for example, on
the eye) unless they are specifically intended
for that purpose.
The instruments and accessories described in
this operating manual have been tested for
safety and potential hazards. The responsible
Leica affiliate must be consulted whenever
the instrument is altered, modified or used in
conjunction with non-Leica components that
are outside of the scope of this manual.
Unauthorized alterations to the instrument or
noncompliant use shall void all rights to any
warranty claims.
Place of use
★ Refer to "Safety Concept" booklet.
★ Electrical components must be placed at
least 10 cm away from the wall and from
flammable substances.
★ Avoid large temperature fluctuations, direct
sunlight and vibrations. These conditions
can distort measurements and micrographic images.
★ In warm and warm-damp climatic zones,
the individual components require special
care in order to prevent the build-up of
fungus.
Leica Z6 APO / Z16 APO User Manual 5
Safety Instructions (cont'd.)
Responsibilities of person in charge of
instrument
★ Refer to "Safety Concept" booklet.
Ensure that:
★ The Z series macroscopes and accessories
are operated, maintained and repaired by
authorized and trained personnel only.
★ All operators have read, understood and
observe this User Manual, and particularly
the safety regulations.
Repairs, service work
★ Refer to "Safety Concept" booklet.
★ Only original Leica Microsystems spare
parts may be used.
★ Before opening the instruments, switch off
the power and unplug the power cable.
★ Touching the live circuit can cause injury.
Transport
★ Use the original packaging for shipping or
transporting the individual modules and
the accessory components.
★ In order to prevent damage from vibra-
tions, all moving parts that (according to
the user manual) can be assembled and
disassembled by the customer should be
disassembled and packed separately.
Integration in third-party products
★ Refer to "Safety Concept" booklet.
Disposal
★ Refer to "Safety Concept" booklet.
Legal requirements
★ Refer to "Safety Concept" booklet.
EC Declaration of Conformity
★ Refer to "Safety Concept" booklet.
Health risks
Workplaces equipped with macroscopes facilitate and improve the viewing task, but they
also impose high demands on the eyes and
holding muscles of the user. Depending on the
duration of uninterrupted work, asthenopia
and musculoskeletal problems may occur. For
this reason, appropriate measures for reduction
of the workload must be taken: Optimal arrangement of workplace, work assignments and
work flow (changing tasks frequently).
★ Thorough training of the personnel, giving
consideration to ergonomic and organizational aspects.
The ergonomic design and construction of the
Leica Z series are intended to reduce the exertion of the user to a minimum.
Direct contact with eyepieces can be a potential transmission path for bacterial and viral
Leica Z6 APO / Z16 APO User Manual 6
Safety Instructions (cont'd.)
infections of the eye. The risk can be kept to a
minimum by using personal eyepieces for each
individual or detachable eyecups.
Light sources: Safety Instructions
Protective measures of the manufacturer:
★ UV protection screen in front of the speci-
men plane prevents the user from looking
directly into the UV rays.
★ Dummy filter carriers in the free positions
of the rapid filter changer prevent direct UV
radiation from reaching the eyes.
★ UV filters are installed in the observation
beam paths to protect the eyes.
★ The stray-light protection on the lamp
housing prevents irradiation of the hands.
Warning
UV radiation could damage the eyes. Therefore:
★ Never look into the light spot on the speci-
men plane without a UV protection screen.
★ Never look into the eyepieces if no excita-
tion filter is in the beam path.
★ Fill empty filter positions with dummy filter
carriers.
★ Do not select a white, strongly reflective
background for the specimen.
Supply unit
Always unplug the supply unit from the power
supply:
★ When installing and disassembling the
lamp housing
★ Before opening the lamp housing
★ When replacing the high-pressure mercury
lamp and other parts, such as the heatabsorbing filter or the collector
★ During maintenance work on the supply unit
Leica Z6 APO / Z16 APO User Manual 7
Safety Instructions (cont'd.)
Lamp housing
★ Never open the lamp housing while the
lamp is switched on. Risk of explosion, UV
exposure, blinding!
★ Before opening the lamp housing, allow it
to cool off for at least 15 minutes. Danger
of explosion!
★ Never cover the air duct on the lamp
housing. Danger of fire!
Mercury lamp
★ Follow the user manual and safety instruc-
tions provided by the lamp manufacturer, and, in particular, the section on how
to proceed if a lamp breaks and releases
mercury.
★ For transport, remove the mercury lamp,
transport it in its original packaging and
protect moving parts in the lamp housing
using the transport anchors.
★ When it has reached the end of its rated life
(follow the manufacturer's specifications
and the minute meter on the supply unit).
★ To minimize the risk of explosion, replace a
discolored mercury lamp promptly
★ Leica Microsystems assumes no liability
for damage caused by exploding, incorrectly installed or improperly used mercury
lamps.
Leica Z6 APO / Z16 APO User Manual 8
Table of Contents
The Leica Z Series
Congratulations! 13
The Modular Design: Everything is Relative 14
The Automatic Control System 15
On We Go 16
Assembly
Incident-light Bases and Manual Focusing Column 18
Transmitted-light Bases and Manual Focusing Column 19
Microscope Carrier 20
Video/Phototube A 21
Video/Phototube AS ("Slim") 22
Video/Phototube Y 23
Coaxial Illuminator (Optional) 24
Optics Carrier 25
Fine Focus (Optional) 26
Objective 27
Ergo binocular tube 28
Eyepieces 29
Objective Slide: Assembly 30
Objective Slide: Parcentric Adjustment of the Objectives 32
Transmitted-light Base TL ST 33
Transmitted-light Base TL BFDF: Before First Use 34
Transmitted-light Base TL BFDF 35
TL RC™ / TL RCI™ 36
IsoPro™ Manual Mechanical Stage: Assembly 37
Leica IsoPro™ Motorized Mechanical Stage: Assembly 40
Cables: Connections 44
Cables: Cable Duct 45
Leica LED3000 NVI™ (Near Vertical Illumination) 46
Quick Start Guide
Overview of a Manual Leica Z Series Macroscope (Leica Z16 APO) 48
Eyepieces and Focusing
Magnification Factors of the Eyepieces 50
Using the Eyepieces 51
The Correct Interpupillary Distance 52
Dioptric Correction 53
Reticules 54
Health Notes 55
Focusing 56
Adjusting the Resistance of the Focus Drive 57
Fine Focusing 58
Changing Magnification (Zoom) 59
Enabling and disabling ratchet steps 60
Iris Diaphragm 61
Leica Z6 APO / Z16 APO User Manual 9
Photography & Video
Photography & Video 63
Photo Tubes and C-mounts 64
Trinocular Video/Phototube 50% 65
Trinocular Video/Phototube 100% 66
Objectives and
Optical Accessories
The Different Types of Objectives 68
Bases
Leica TL ST Transmitted-light Base: Controls 70
Leica TL ST Transmitted-light Base: Operation 71
Leica TL ST Transmitted-light Base: Changing Bulbs 72
Leica TL BFDF Transmitted-light Base: Controls 73
Leica TL BFDF Transmitted-light Base: Operation 74
Leica TL RC™ / TL RCI™: Controls 75
Leica TL RCI™: The Deflection Mirror 76
Leica TL RCI™: Color Intensity and Temperature 77
Leica TL RC™ / TL RCI™: Operation 78
Leica TL RCI™: Methods in Transmitted Light 79
Leica TL RCI™: Relief Images 80
Using Filters 82
Leica IsoPro™ (Non-motorized): Controls 83
Leica IsoPro™ (Motorized): Controls 84
System Illumination
Leica LED3000 NVI™ (Near Vertical Illumination) 86
Accessories
Leica PSC Controller 88
Leica SmartTouch 89
Manual Control 90
Footswitch 91
Dimensional Drawings
Leica Z6 APO 93
Leica Z16 APO 94
Objectives 95
Video/phototube AS 96
Y tube 97
Video/Phototube A 98
Microscope carrier 99
Focusing arm for probers 100
Focusing arms 101
Adapters 102
Coaxial incident-light housing 103
Leica Z6 APO / Z16 APO User Manual 10
Specications
Optical Data – Visual with Y Tube 1.25× 105
Leica Z6 APO & Z16 APO 108
Optical Accessories for Leica Z6 APO & Z16 APO 109
Ambient Conditions and Weights 110
Leica TL ST Transmitted-light Base 111
Leica TL BFDF Transmitted-light Base 112
Leica TL RC™ / TL RCI™ 113
Leica IsoPro™ Motorized XY Stage 114
Appendix
Calculating the Total Magnification and Field of View Diameter 116
Care, Maintenance, Contact Persons 117
Leica Z6 APO / Z16 APO User Manual 11
The Leica Z Series
Leica Z6 APO / Z16 APO User Manual 12
Congratulations!
Congratulations on obtaining your new Leica Z series macroscope. We are
convinced it will exceed your expectations, as the Z series embodies all
the qualities you associate with the name Leica Microsystems: excellent
objectives, high-quality engineering, and reliability. Furthermore, the
modular design ensures that the Z series adapts perfectly to your needs—
no matter which accessories you require for your tasks.
Thanks to the parallax error free observation, measurements are more
accurate and adaptation tasks are easier. Together with a Leica digital
camera, you obtain the maximum amount of high-quality data, which
also makes later evaluations on the computer much easier.
Though the reliability and robustness of Leica macroscopes is legendary,
like any high-tech product, the Leica Z series requires a certain degree of
care and attention. Therefore, we recommend that you read this manual.
It contains all the information you need regarding operation, safety and
maintenance. Simply observing a few guidelines will ensure that even
after years of intensive use, your Leica macroscope will continue to work
as smoothly and reliably as on the very first day.
We wish you the best of success in your work— after all, you are now
equipped with the best tool!
Leica Z6 APO / Z16 APO User Manual 13
The Modular Design: Everything is Relative
The Leica Z series provides maximum flexibility
in choosing equipment, thanks primarily to the
modular configuration and the compatibility
that Leica has painstakingly maintained for
decades. The optics carriers, eyepieces, bases
and more can be combined in any way you
choose, allowing you to create the macroscope
that best suits your needs.
Despite this, you will notice that the controls
and individual components do not differ significantly. Whichever configuration you choose,
you will quickly feel right at home with your
new macroscope.
Have a special request? Let us know!
Leica Microsystems enjoys an exceptional repu-
tation when it comes to devising customerspecific solutions. If you have a special request
that cannot be met with standard parts, contact
your Leica consultant. He or she has a solution
for every problem.
Leica Z6 APO / Z16 APO User Manual 14
The Automatic Control System
Reliability for your experiments
For the Leica Z6 APO A and Z16 APO A automated macroscopes, motorized control of the
zoom, iris diaphragm and fine focus is possible.
This guarantees not only convenient work, but
also added reliability for your experiments, as
results can be reproduced exactly. In conjunction with the Leica LED3000 NVI™ system illuminators, a wide variety of scenarios can be set up,
saved and restored at the touch of a button.
Control à la carte
You alone determine the degree of automation.
The zoom, focus and illumination can be optionally controlled using the "Leica Application
Suite" (LAS) software solution or via the Leica
SmartTouch touchscreen panel.
To automate your workflows even more, also
integrate the Leica IsoPro™ motorized XY stage
– it positions multiple specimens fully automa-
*
tically
or turns a series of individual images
into one large, seamless illustration
**
, to give
only two examples.
Talk to your Leica partner about the wide range
of automation possibilities – he or she will show
you how to get the maximum out of your Leica
macroscope.
* With LAS auxiliary module LAS MultiStep
** With LAS auxiliary module LAS PowerMosaic
The Leica SmartTouch controls all motorized
functions of the Z series and the connected
The Leica Application Suite (LAS) controls and
photographs fully automatically as desired.
accessories.
Leica Z6 APO / Z16 APO User Manual 15
On We Go
If your new Leica macroscope has already been
assembled and commissioned by your Leica
consultant, click here to skip through the installation instructions and go directly to the Quick
Start Guide on page 47.
If, on the other hand, you are assembling the
Leica macroscope yourself, continue with the
"Assembly" chapter, which begins on Page 17.
Leica Z6 APO / Z16 APO User Manual 16
Assembly
Leica Z6 APO / Z16 APO User Manual 17
Incident-light Bases and Manual Focusing Column
The first step is to connect the focusing column
to the corresponding base.
Tools used
★ Allen key provided
Assembling the focusing column
1. Lay the focusing column on the edge of a
table or another stable substrate so that
the three threads at the end of the column
protrude over the edge.
2. Hold the incident-light to the focusing
column and secure it to the base using the
three screws provided.
Tip: The base can be installed more easily
if someone helps you do so.
Leica Z6 APO / Z16 APO User Manual 18
Transmitted-light Bases and Manual Focusing Column
For additional information on the TL
bases, refer to page 33.
The first step is to connect the focusing column
to the corresponding base.
Tools used
★ Allen key provided
Assembling the column adapter
1. Securely install the column adapter on the
column using the four included screws.
Assembling the focusing drive
2. Securely screw the focusing column to the
base using the six included screws.
Leica Z6 APO / Z16 APO User Manual 19
Microscope Carrier
Tools used
★ Allen key provided
Assembling the Microscope Carrier
1. Press the microscope carrier against the
focusing column.
2. Tighten the screw using the Allen key provided.
Leica Z6 APO / Z16 APO User Manual 20
Video/Phototube A
The video/phototube A is used if only a
Leica digital camera with the Leica LAS
(Leica Application Suite) software is used to
observe the specimen.
Assembling the tube
1. Unscrew the locking screw on the optics
carrier.
2. Insert the tube into the microscope carrier.
3. Tighten the locking screw on the microscope carrier.
Installing the objective/camera
1. Screw the desired digital camera to the
video objective.
2. Insert the video objective into the video/
phototube A and turn the knurled ring until
tight.
Leica Z6 APO / Z16 APO User Manual 21
Video/Phototube AS ("Slim")
The video/phototube AS is intended for
OEM applications and is usually not used
on Leica focusing columns.
After installation on the OEM equipment,
the rest of the components are installed
as described on the following pages.
Leica Z6 APO / Z16 APO User Manual 22
Video/Phototube Y
You can use the Y-tube to attach binocular tubes, ErgoModules®, video/phototu-
bes and the Leica IC A video module of the Leica
M series.
Assembling the tube
1. Unscrew the locking screw on the optics
carrier.
2. Insert the tube into the microscope carrier
so that the locking screw faces the rear.
3. Tighten the locking screw on the microscope carrier.
Assembling the tube
1. Insert the desired tube and turn the knurled
ring until tight (refer also to page 28).
Leica Z6 APO / Z16 APO User Manual 23
Coaxial Illuminator (Optional)
Tools used
★ Allen key provided
The coaxial illuminator is fitted between
the optics carrier and the tube. It enables
you to connect the light guide, which, in turn,
is connected to a cold light source of the Leica
CLS series.
At low magnifications, using the coaxial
illuminator causes vignetting (shading),
which is more or less pronounced depending
on the configuration. This vignetting is not a
malfunction.
The quarter-wave plate for coaxial illumination must be installed on the planapo-
chromatic objectives.
Installing the coaxial illuminator
1. Guide the coaxial illuminator to the tube
from the left or right.
2. Adjust the coax illuminator so that the port
for the light guide faces the direction that is
the most comfortable for your work.
3. Push the coaxial illuminator upwards and
tighten the screw on the coaxial illuminator.
4. Connect the light guide of the Leica cold
light source to the coaxial illuminator.
The User Manual for the Leica cold light
source is provided with the cold light
source.
Leica Z6 APO / Z16 APO User Manual 24
Optics Carrier
Tools used
★ Allen key provided
The instructions on this page also apply
logically if the optional coaxial illumi-
nator has been installed (see page 24). In this
case, the optics carrier is attached to the coaxial
illuminator instead of the tube.Assembling the
optics carrier
1. Unscrew the fastening screw on the optics
carrier.
2. Guide the optics carrier to the tube from
the rear.
3. Push the optics carrier upwards and tighten
the fastening screw.
Leica Z6 APO / Z16 APO User Manual 25
Fine Focus (Optional)
Tools used
★ Allen key provided
The optional fine focus allows sensitive
and precise focusing in a range of 10 mm.
The fine focusing is required for precise focusing at high magnifications.
Before the fine focus is installed, the
diaphragm ring must be removed from
the optics carrier.
Removing the diaphragm ring
1. Unscrew the 3 fastening screws on the
diaphragm ring of the optics carrier.
2. Remove the diaphragm ring.
Installing the fine focus
1. Place the fine focus on the optics carrier.
2. Tighten the three fastening screws.
Leica Z6 APO / Z16 APO User Manual 26
Objective
Tools used
★ None
You can attach the quarter-wave plate
for coaxial illumination, the analyzer for
polarization or a ring illuminator to the planapochromatic objectives.
HR and DM objectives are high-performance objectives and always cause
vignetting. This vignetting is not a malfunction.
Attaching the objective
1. Screw the planapochromatic objective to
the diaphragm ring or the optional fine
focus in a counterclockwise direction.
Alternative fastening options
★ If using the objective slide, read the instruc-
tions on page 30.
Leica Z6 APO / Z16 APO User Manual 27
Ergo binocular tube
Tools used
★ No tools required.
Prerequisite for the installation
When using the ergo binocular tube or
another tube, the Y video/phototube
must be used.
Preparations
1. Unscrew the positioning screw and remove
the protective cover.Assembling the tube
1. Push the tube into the dovetail ring and
rotate it slightly in both directions until the
positioning screw meshes with the guide
groove.
2. While holding the tube only slightly, carefully tighten the positioning screw. It is
automatically brought to the correct position.
Leica Z6 APO / Z16 APO User Manual 28
Eyepieces
Tools used
★ None
Magnification range
You can extend the overall magnification
range using available 10×, 16×, 25× and
40× wide-field eyepieces for persons wearing
glasses.
Preparation
1. If you want to use an optional graticule,
insert it now (Page 54).
2. Remove the plastic tube guard.
Inserting the eyepieces
3. Push the eyepieces into the tubes as far as
they will go and check to ensure that they
fit tightly and accurately.
4. Securely tighten the clamping screws.
Leica Z6 APO / Z16 APO User Manual 29
Objective Slide: Assembly
Supported objectives
2.0× planapochromatic objective
5.0× planapochromatic objective
You can identify the centerable objective
thread by the movable ring and the 4
centering screws on the front side of the ring.
Orient the objective slide during installation, preferably at an angle of 90°, so that
the illuminator and the specimen are optimally
accessible. The centerable objective thread is
oriented towards the user.
Installing the objective slide:
1. Unscrew the 3 fastening screws on the
diaphragm ring of the optics carrier.
2. Remove the diaphragm ring.
(Continued on next page)
Leica Z6 APO / Z16 APO User Manual 30
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