Leica WATER IMMERSION MICRO DISPENSER User Manual

Water Immersion
Micro Dispenser

Automatic supply of water immersion during experiments

Small and compact design allows utilization of all nosepiece
positions. A retrofit to existing Leica DMI6000 B is possible.

Add new Applications
to your System

Use the best matching objective

Live cell research and screening applications are mainly based
on samples in an aqueous solution, so the use of water objectives
is becoming more and more important. Water objectives allow
for aberration free focusing into such samples. Moving into the
sample will cause no loss of contrast.

Additional Benefits

No interaction required during the experi-

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ment – Software controlled water immer­sion supply
No water damage to the instrument –

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Water protection collar provided
Prevents disruption of water film during

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stage movement – Intelligent design of
water cap
Ensures best image quality – Accessibility

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of correction ring
Perform your experiments with no restric-

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tions – Full free working distance available
No separate heating required – Water

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reservoir and micro pump fit into climate
chamber

Unfortunately water evaporates at room temperature and there­fore cannot be used for long term experiments. During screening
or mark and find experiments the immersion film can be disrupted.
The Water Immersion Micro Dispenser overcomes these problems
by adding immersion automatically during a running experiment.

This allows for applications with high resolution water objectives,
for example:

Live-cell experiments at 37°C

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Long-term live-cell experiments

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Screening

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Well plate acquisition.

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In addition the user does not have to add water immersion manu­ally. It can be added at the press of a button via the convenient
control software supplied with the Water Immersion Micro Dis­penser.

Multi-position time-lapse experiment covering 32 wells of a 96 well plate. Single time point of a data set with an acquisition time of 72 hours using a HCX PL
APO 20x/0.70 IMM CORR CS objective with a Water Immersion Micro Dispenser attached to overcome water evaporation. Cells described in Steigemann et al.;
Cell. 2009 Feb 6;136(3):473-84. Courtesy of Dr. Daniel Gerlich (ETH Zürich), Jutta Bulkescher and Dr. Stefan Terjung (ALMF, EMBL Heidelberg).

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