Leica VT1200/VT1200 S V 1.4 RevC, English - 09/2013
Order No. 14 0481 80101 RevC
Always keep this manual with the instrument.
Read carefully before working with the instrument.
Note
The information, numerical data, notes and value
judgments contained in this manual represent
the current state of scientific knowledge and
state-of-the-art technology as we understand it
following thorough investigation in this field. We
are under no obligation to update the present
manual periodically and on an ongoing basis according to the latest technical developments, nor
to provide our customers with additional copies,
updates etc. of this manual.
To the extent permitted in accordance with
the national legal system as applicable in each
individual case, we shall not be held liable for
erroneous statements, drawings, technical illustrations etc. contained in this manual. In particular, no liability whatsoever is accepted for any
financial loss or consequential damage caused
by or related to compliance with statements or
other information in this manual.
Statements, drawings, illustrations and other
information as regards contents or technical
details of the present Instructions for Use are not
to be considered as warranted characteristics of
our products.
These are determined only by the contract
provisions agreed between ourselves and our
customers.
Leica reserves the right to change technical
specifications, as well as manufacturing processes, without prior notice. Only in this way is
it possible to continuously improve the technology and manufacturing techniques used in our
products.
This document is protected under copyright laws.
Any copyrights of this document are retained by
Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or
of any parts thereof) by means of print, photocopy, microfiche, web cam or other methods
– including any electronic systems and media
– requires express prior permission in writing by
Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the nameplate on
the back of the instrument.
Useful tips,
i.e. important user information, appear
in a gray box and are marked by an .
(5)
(Fig. 5)
Numbers in parentheses refer to item
numbers in illustrations or to the illustrations themselves.
Manufacturer
Date of Manufacture
The CE labeling shows that the product
corresponds to one or more applicable
European directives.
Observe the Instructions for Use
Order No.
Serial number
Environmental protection symbol of
the China RoHS directive.
The number in the symbol indicates
the "Environment-friendly Use Period"
of the product in years.
The symbol is used if a substance
restricted in China is used in excess
of the maximum permitted limit.
The CSA test mark means that a
product has been tested and fulfills
the applicable safety and/or performance standards, including the
relevant standards defined or administered by the American National
Standards Institute (ANSI), Underwriters Laboratories (UL), the Canadian Standards Association (CSA),
the National Sanitation Foundation
International (NSF) and others.
Symbol for labeling electrical and
electronic equipment in accordance
with Section 7 of the German Electrical and Electronic Equipment Act
(ElektroG).
ElektroG is the law on the bringing
into circulation, return and environmentally compatible disposal of electrical and electronic equipment.
Instrument
model:
4
All information provided in these Instructions for Use applies only to the instrument
type indicated on the title page.
A nameplate is attached to the rear side of the instrument. The series and REF numbers are attached to a separate label (Fig. 1a) on the right side of the instrument.
Fig. 1 and 1a are provided as an example only and show a valid nameplate for this
instrument.
Instructions for Use V 1.4 RevC – 09/2013
Fig. 1a
1.2 Qualification of personnel
The Leica VT1200 and the VT1200 S may be operated by trained laboratory
personnel only.
All laboratory personnel designated to operate this instrument must read
these Instructions for Use carefully and must be familiar with all technical
features of the instrument before attempting to operate it.
1. Important Information
Fig. 1
1.3 Intended use/improper use
The Leica VT1200 and VT1200 S are used for sectioning in the fields of medicine, biology and industry, and are especially designed for sectioning fixed
or unfixed fresh tissue in buffer.
The instrument must be used exclusively according to the instructions contained in these Instructions for Use.
Any other use of the instrument is considered improper.
Leica VT1200 / VT1200 S
The VT1200/VT1200 S may be used for research purposes only. Sections made using the VT1200/VT1200 S must NOT be used for diagnostics.
5
2. Safety
These Instructions for Use includes important information related to the
operating safety and maintenance of the instrument.
The Operating Manual is an important part of the product, and must be
read carefully prior to startup and use and must always be kept near the
instrument.
If additional requirements on accident prevention and environmental protection apply in the country of operation, these Instructions for Use must
be supplemented by appropriate instructions to ensure compliance with
such requirements.
Make sure to read all of these Instructions for Use before you work on or
operate the instrument.
2.1 General safety notes
These instruments have been built and tested in accordance with the safety
regulations for electrical measuring, control, regulating and laboratory
devices.
In order to maintain this condition and to ensure safe operation, the user
must follow the instructions and warnings contained in this operating manual.
2.2 Warnings
6
The current EC Declarations of Conformity can be found on the Internet:
www.LeicaBiosystems.com
The safety devices installed in this instrument by the manufacturer only
constitute the basis for accident prevention. Operating the instrument safely
is, above all, the responsibility of the owner, as well as the designated personnel who operate, service or clean the instrument.
To ensure trouble-free operation of the instrument, make sure to comply
with the following instructions and warnings.
Instructions for Use V 1.4 RevC – 09/2013
• Extremely sharp blades pose risk of injury when touched!
• Fresh tissue poses risk of infection!
• Fire hazard from uncovered magnifier! Cover the magnifier during work breaks!
Proper handling
Always be exceptionally careful when handling the blades!
Do not leave open blades lying around after removal.
Always make sure to handle the blade in a way that will not cause you injury.
All appropriate safety precautions must be met to avoid the risk of infection.
Wearing safety gloves, a mask and safety goggles—in accordance with the "Working with Sub-
stances that Pose a Health Risk" guidelines—is absolutely mandatory.
The instrument may be opened by authorized service personnel only.
Always disconnect the power plug before opening the instrument.
Always switch off the instrument using the power switch and disconnect the power plug before
replacing the fuse. The use of fuses other than those installed at the factory is not permitted.
2.3 Transport, unpacking and setting up
2. Safety
The instrument must be set up so that the main power switch on its right side (item 7 in Fig. 14) is
easily accessible at any time.
Because the weight of the instrument is approx. 56 kg, carrying the instrument requires 2 persons
(1 carrying handle per person).
Total vertical specimen stroke .................................................................................................20 mm (motorized)
Cutting range ..............................................................................................................................45 mm (adjustable)
Maximum specimen size:
With standard blade holder ............................................................................................................. 33 x 50 mm
Height: ........................................................................................................................ up to 2000 m above sea level
Electrical data:
Rated voltage range (± 10 %): ....................................................................................................................... 100 V - 240 V
Nominal frequency (±10 %): .................................................................................................................................. 50/60 Hz
Power consumption: ...................................................................................................................................................35 VA
Power fuse: ...................................................................................................................................................... T 1 A L 250 V
Overvoltage category: ....................................................................................................................................................... II
Internal current limit for the electronics: ................................................................................................................... Yes
Dimensions:
L x W x H: ............................................................................................................................... 600 mm x 250 mm x 230 mm
Height with magnifier support ............................................................................................ 600 mm x 250 mm x 320 mm
Height with microscope: ..................................................................................................... 600 mm x 250 mm x 490 mm
L x W x H control unit (when the bases are folded in): ................................................... 165 mm x 120 mm x 72 mm
Weight:
Without magnifier support and control unit .............................................................................................................56 kg
VT1200 control unit .........................................................................................................................................................1 kg
Magnifier support ...........................................................................................................................................................2 kg
Microscope support with stereomicroscope ............................................................................................................4 kg
8
Instructions for Use V 1.4 RevC – 09/2013
3. Instrument Characteristics
3.1.1 Technical data for the VT1200 S
General data:
Sectioning frequency (± 10 %): ...................................................................................................................85 Hz (± 10 %)
Amplitude: ....................................................................................................... from 0 - 3 mm, in increments of 0.05 mm
Return speed (± 10 %): .................................................................................... 1.0 - 5 mm/s, in increments of 0.5 mm/s
Total vertical specimen stroke: ......................................................................................................... 20 mm (motorized)
Sectioning range: ..................................................................................................................................................... 45 mm
Sectioning window: .................................................................................................................................. 0.5 mm - 45 mm
Specimen retraction: .............................................................................. 0 - 100 µm (adjustable; can be deactivated)
Maximum specimen size:
with standard blade holder: ..................................................................................................................... 33 x 50 mm
Sectioning thickness setting: ............................................manual in 1 µm increments or automatic max. 1000 µm
Ambient conditions:
Operating temperature range: .....................................................................................................min. 10 °C - max. 35 °C
Height: .................................................................................................................................up to 2000 m above sea level
Electrical data:
Rated voltage range (± 10 %): ...................................................................................................................... 100 V - 240 V
Nominal frequency (± 10 %): ................................................................................................................................ 50/60 Hz
Power consumption: ..................................................................................................................................................35 VA
Power fuse: ..................................................................................................................................................... T 1 A L 250 V
Overvoltage category: ...................................................................................................................................................... II
Internal current limit for the electronics: .................................................................................................................. Yes
Dimensions:
L x W x H: .............................................................................................................................. 600 mm x 250 mm x 230 mm
Height with magnifier: ......................................................................................................... 600 mm x 250 mm x 320 mm
Height with microscope: .................................................................................................... 600 mm x 250 mm x 490 mm
Control unit (when the bases are folded in): ..................................................................... 190 mm x 150 mm x 72 mm
Weight:
Without magnifier support and control unit: ...........................................................................................................56 kg
VT1200 S control unit: ...................................................................................................................................................1 kg
Magnifier support: .........................................................................................................................................................2 kg
Microscope support with stereomicroscope: ..........................................................................................................4 kg
Leica VT1200 / VT1200 S
9
3. Instrument characteristics
3.2 General overview – VT1200/VT1200 S
Fig. 3, Magnifier
Fiber
optic
light guide
Cold light
source
Fig. 4, Microscope
Fig. 5
Cutting head
Fig. 2, Basic instrument
10
Fig. 6,
Foot switch
Blade holder
Fig. 8,
Control panelm
VT1200 Smm
Fig. 7,
VT1200 control
panel
Instructions for Use V 1.4 RevC – 09/2013
Attachments on dovetail receptacle
3. Instrument Characteristics
Ice tray
Buffer tray
Buffer tray,
plastic
Buffer tray,
metal
Specimen plate
for specimens 1 c m
in height
for specimens 2 cm
in height
Double-walled buffer tray
Julabo FL300
(recirculating
cooler/chiller)
Specimen plate
for specimens 1 c m
in height
for specimens 2 cm
in height
VibroCheck
Fig. 9
Blades for the blade holder
Sapphire blade
Directional
Cyanoacrylate adhesive
Leica VT1200 / VT1200 S
Directional
Cyanoacrylate adhesive
11
3. Instrument characteristics
3.3 VT1200 Control Panel
LED display for blade feed
rate and amplitude
Setting dial 1, for blade feed
rate and amplitude
LED display for section
thickness and
section thickness totalizing
Setting dial 2, for section
thickness and moving the
specimens vertically
Selection of the step size,
1 µm,10 µm or
Quickly moves the speci-
men upwards (press the
button until the desired
Quickly moves the specimen
downwards (press once to
adjust to the lowest position)
(for approx. 3 sec.) to save a
frequently used feed value
(pressing it more than once
feeds by the saved section
thickness multiple times).
100 µm steps
position is reached)
Press this button
Moves the blade to-
wards the specimen
Caution: Keep it pressed down until the desired
position is reached. When the end position is
reached, the LED lights up.
LED for feed rate
LED on, for enabled ampli-
1
2
Moves the blade away
from the specimen
tude adjustment
Toggles between SPEED (blade
feed rate) and AMPL (amplitude)
Section thickness
Sets the current specimen
position between 0 (lower) and
20,000 µm (upper) or - after zero,
totals the section thicknesses
Pressing this button toggles
between µm and ∑µm (actively illuminated).
Resets the totalized section
thickness display ∑µm to zero.
Immediately starts or stops
the sectioning process
ON/OFF lamp
Fig. 10
12
The Leica VT1200 is a semiautomatic microtome with a vibrating blade. Before each cut, a manual feed to the desired section thickness must be carried out using the setting dial for section
thickness. The VT1200 does not include an automatic specimen retraction; however, retraction
can be performed manually.
Instructions for Use V 1.4 RevC – 09/2013
3.3.1 VT1200 S control panel
Sets the blade feed rate.
Select the values using set-
ting dial 1.
Setting dial 1, for blade
feed rate (SPEED),
AMPLitude or section
thickness for automatic
mode (AUTO FEED)
Sets the amplitude.
Select the values using
setting dial 1.
3. Instrument Characteristics
Possible in automatic sectioning
mode only. Select the desired section thickness for the automatic
feed using setting dial 1.
ON/OFF lamp
Setting dial 2, for section
thickness and moving the
specimens vertically
Selection of the step size,
1 µm, 10 µm or 100 µm steps
First cutting window edge,
LED illuminates when the
window edge is set
Moves the specimen up-
wards (as long as the button
is pressed). When the up-
permost position is reached,
the LED lights up.
Second cutting window
edge, LED illuminates when
the window edge is set
Fig. 11
Toggles between automatic (AUTO) and
semiautomatic (MAN) sectioning mode
The Leica VT1200 S is a fully automatic microtome with vibrating blade. It can be operated in automatic as well as
semiautomatic sectioning mode.
In semiautomatic sectioning mode, a manual feed to the desired section thickness must be carried out before
each cut. There is no automatic specimen retraction in this mode; however, retraction can be performed manually.
In automatic mode, an automatic feed (AUTO FEED) to the selected section thickness is carried out before each
cut, and the specimen is lowered to the desired retraction value after each cut to prevent the specimen surface
and the blade from coming into contact while the blade is being retracted.
Moves the speci-
men into the lowest
position, (LED is
illuminated when the
lowest position is
reached)
Moves the
blade towards
the specimen
Caution: Keep it pressed
down until the desired position is reached. When the
end position is reached, the
LED lights up.
Moves the blade
away from the
specimen
Toggles in and out of the
menu. Memory for 8 parameter sets.
Sets the totalized section
thickness display åµm to "0".
Interrupts the sectioning
process in automatic mode.
Pressing the button again
reactivates the sectioning
process.
Starts or stops the sectioning process. In semiautomatic
mode, the sectioning process
stops immediately; in automatic
mode, the sectioning process is
finished completely.
Toggling between single
stroke (SINGLE) and continuous stroke (CONT) in automatic
mode is possible. In semiautomatic mode, only single stroke
(SINGLE) is possible.
When ordering additional accessories, compare the parts received
with the parts ordered. If the parts received do not match your order,
contact the sales company responsible for your order immediately.
Instructions for Use V 1.4 RevC – 09/2013
4.1.1 Standard scope of delivery for the VT1200 S
VT1200 S basic instrument ............................................................. 14 0481 42066
1 control panel ................................................................................ 14 0481 43396
1 toolset:
- 1 Allen key, size 3.0 ................................................................. 14 0194 04764
- 1 Allen key, size 6.0 ................................................................. 14 0222 04141
When ordering additional accessories, compare the parts received
with the parts ordered. If the parts received do not match your order,
contact the sales company responsible for your order immediately.
15
4. Installation
4.2 Packing and setting up the instrument
Ensure that the instrument is standing on a work surface that is as free
of vibrations as possible.
Before each transport, the handles must be screwed onto the instrument using the
provided screws (see Fig. 13). Check to ensure that the handles are firmly in place
and will hold reliably!
Packing the instrument
1. Have 2 people grab the instrument by the transport handles (1), place it
1
2
1
2
3
on the wooden pallet and screw it to the pallet using the 4 size 6 Allen
screws (2).
2. Pull the transparent protective hood (3) over the instrument. Place the
wooden box (4) on the baseplate. Insert the inner carton ring (5).
3. Insert the accessories box (6 – contains accessories) and fill it with
packing material (7).
4. Attach the cover (8) and screw it into place using 8 Phillips screws (9).
5
16
4
6
7
8
9
Instructions for Use V 1.4 RevC – 09/2013
9
Fig. 12
4.3 Before commissioning the instrument
1
1
Fig. 13
2
7 (AN)
4
4. Installation
Once the instrument is in its final location, unscrew the transport handles (1) from the instrument using the size 6 Allen key provided and
store them, along with the screws, in a safe
place.
1. Set the power switch (7) on the right side of
the instrument to OFF ().
2. Make sure the power cable is correctly connected in the power socket (2) on the right side
of the instrument.
3. Connect the control panel to the socket (3).
4. Install the ice tray (4).
5. Install the buffer tray (5).
6. Install any optional. accessories, such as the
magnifier, microscope, foot switch, etc. according to p. 49.
7. Use the power switch (7) to switch on the
instrument.
3
Fig. 14
Leica VT1200 / VT1200 S
To make it easier to insert the specimen, adjust the specimen receptacle to
its lowest position, and adjust the blade
5
6
holder to its rearmost position during
the first reference run.
17
Loading...
+ 43 hidden pages
You need points to download manuals.
1 point = 1 manual.
You can buy points or you can get point for every manual you upload.