Leica S series User Manual

Living up to Life
User Manual
Leica S series
General Instructions
Safety concept
Before using your stereomicroscope for the first time, please read the "Safety concept" brochure included with your instrument. It contains additional information about handling and care.
Use in clean rooms
The Leica S series can be used in clean rooms without any problems.
About cleaning
O Do not use any unsuitable cleaning agents,
chemicals or techniques for cleaning.
O Never use chemicals to clean colored
surfaces or accessories with rubberized parts. This could damage the surfaces, and specimens could be contaminated by abra­ded particles.
O In most cases, we can provide special solu-
tions on request. Some products can be modified, and we can offer other accesso­ries for use in clean rooms.
Servicing
O Repairs may only be carried out by Leica
Microsystems-trained service technicians. Only original Leica Microsystems spare parts may be used.
Responsibilities of person in charge of instrument
O Ensure that the Leica stereomicroscope
is operated, maintained and repaired by authorized and trained personnel only.
Leica S Series User Manual 2
Important Safety Notes
User Manual
The individual modules of the Leica stereomi­croscope include an interactive CD-ROM with all relevant user manuals in several languages. Keep it in a safe place, and readily accessible to the user. User manuals and updates are also available for you to download and print from our web site www.leica-microsystems.com.
This User Manual describes the special func­tions of the Leica StereoZoom® stereomicro­scopes (S series) and contains important inst­ructions for their operational safety, maintenance, and accessories.
The "Safety Concept" booklet contains additi­onal safety information regarding the service work, requirements and the handling of the stereomicroscope, accessories and electrical accessories as well as general safety instruc­tions.
You can combine individual system articles with articles from external suppliers (e.g. cold light sources, etc.). Please read the user manual and the safety requirements of the supplier.
Before installing, operating or using the instru­ments, read the user manuals listed above. In particular, please observe all safety instructions.
To maintain the unit in its original condition and to ensure safe operation, the user must follow the instructions and warnings contained in these user manuals.
Leica S Series User Manual 3
Symbols Used
Warning of a danger
This symbol indicates especially impor-
tant information that is mandatory to
read and observe.
Failure to comply can cause the following:
O Hazards to personnel O Functional disturbances or damaged inst-
ruments
Warning of hazardous electrical voltage
This symbol indicates especially impor-
tant information that is mandatory to
read and observe.
Failure to comply can cause the following:
O Hazards to personnel O Functional disturbances or damaged inst-
ruments
Danger due to hot surface.
This symbol warns against touching hot
surfaces, e.g. those of light bulbs.
Important information
This symbol indicates additional infor-
mation or explanations that intend to
provide clarity.
Explanatory notes
O This symbol within the text stands for addi-
tional information and explanations.
Figures
(1) Numbers in parentheses within the
descriptions relate to the figures and the items within those figures.
Leica S Series User Manual 4
Safety Instructions
Description
The individual modules fulfill the highest requi­rements for observation and documentation of Leica stereomicroscopes of the S series.
Intended use
O Refer to "Safety Concept" booklet
Non-intended use
O Refer to "Safety Concept" booklet
Never use S series microscopes or their compo­nents for surgical procedures (e.g. on the eye) unless they are specifically intended for that purpose.
The devices and accessories described in this User Manual have been tested with regard to potential hazards. The responsible Leica affiliate must be consulted whenever the instrument is altered, modified or used in conjunction with non-Leica components that are outside of the scope of this manual!
Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims!
Place of use
O Refer to "Safety Concept" booklet
O Electrical components must be placed at
least 10 cm away from the wall and from flammable substances.
O Avoid large temperature fluctuations,
direct sunlight and vibrations. These condi­tions can distort measurements and micro­graphic images.
O In warm and warm-damp climatic zones,
the individual components require special care in order to prevent the build-up of fungus.
Responsibilities of person in charge of instrument
O Refer to "Safety Concept" booklet
Ensure that:
O The S series stereomicroscopes and acces-
sories are operated, maintained and repaired by authorized and trained person­nel only.
O All operators have read, understood and
observe this User Manual, and particularly the safety instructions.
Leica S Series User Manual 5
Safety Instructions (continued)
Repairs, service work
O Refer to "Safety Concept" booklet
O Only original Leica Microsystems spare
parts may be used.
O Before opening the instruments, switch off
the power and unplug the power cable.
Touching the live circuit can cause
injury.
Transport
O Use the original packaging for shipping or
transporting the individual modules of the Leica S stereomicroscopes and the acces­sory components.
O In order to prevent damage from vibra-
tions, all moving parts that (according to the user manual) can be assembled and disassembled by the customer should be disassembled and packed separately.
Integration in third-party products
O Refer to "Safety Concept" booklet
Disposal
O Refer to "Safety Concept" booklet
Legal regulations
O Refer to "Safety Concept" booklet
EC Declaration of Conformity
O Refer to "Safety Concept" booklet
Leica S Series User Manual 6
Safety Instructions (continued)
Health risks
Workplaces with stereomicroscopes
•
facilitate and improve the viewing task, but they also impose high demands on the eyes and holding muscles of the user. Depending on the duration of uninterrupted work, asthenopia and musculoskeletal problems may occur. For this reason, appropriate measures for reduction of the workload must be taken:
O Optimal arrangement of workplace, work
assignments and work flow (changing tasks frequently).
O Thorough training of the personnel, giving
consideration to ergonomic and organizati­onal aspects.
O The ergonomic optical design and const-
ruction of the Leica S stereomicroscopes are intended to reduce the exertion of the user to a minimum.
Direct contact with eyepieces is a
•
potential transmission method for
bacterial and viral infections of the eye.
The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups.
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Table of Contents
General Instructions 2 Important Safety Notes 3 Symbols Used 4 Safety Instructions 5 Table of contents 8
Leica S Series
Congratulations! 11 The Modular Design: Everything is Relative 12 What your stereomicroscope has to offer you 13 The model series 14 On We Go 15
Assembly
Installing the basic equipment (overview) 17 Focusing column 18 Sub-base for transmitted light and cold light source 19 Optics carrier and additional objective 20 Available graticules 21 Inserting graticules 22 Eyepieces 23 Leica LED illuminators 24 Mounting a camera (Leica S6 D and S8 APO) 25
Quick Start Guide
A stereomicroscope of the S series in overview 27 Tips for working ergonomically 28 Using the Eyepieces 29 The correct interpupillary distance 30 Focusing 31 Switching magnification (zooming) 32 Limiting zoom range 33 Regulating the resistance of the focusing drive 35 Changing the position of the optics carrier 36 Diopter settings and parfocality: 1 adjustable & 1 fixed eyepiece 37 Dioptric correction with two adjustable eyepieces 40
Photography & Video
Photography & Video 44 Photography with the Leica S6 D and S8 APO 45
Dimensional drawings in mm
Leica S6 E (S4 E / S6 T) with incident light and transmitted light illumination 47 Leica S6 48 Leica S6 D with incident light and transmitted light illumination 49 Leica S8 APO with incident light and transmitted light illumination 50
Leica S Series User Manual 8
Specifications
Overview of technical features 52 Specifications 53
Appendix
Calculating the total magnification/field of view diameter 55 Troubleshooting 56 Care, Maintenance, Contact Persons 57
Leica S Series User Manual 9
Leica S series
Leica S Series User Manual 10
Congratulations!
Congratulations on obtaining your new Leica StereoZoom® line stereomi­croscope (S series). We are convinced it will exceed your expectations, as this instrument embodies all the qualities you associate with the name Leica Microsystems: excellent objectives, high-quality engineering, and reliability. Furthermore, the modular design ensures that the Leica stereo­microscope adapts perfectly to your needs – no matter which accessories you require for your tasks.
Thanks to the parfocal system with simultaneously large working distan­ces and object fields, you can always view your microscopic specimens accurately – from the complete image to the finest detail.
Though the reliability and robustness of Leica stereomicroscopes is world­renowned, like any high-tech product, the Leica S series requires a certain degree of care and attention. Therefore, we recommend that you read this manual. It contains all the information you need regarding operation, safety and maintenance. Simply observing a few guidelines will ensure that even after years of intensive use, your stereomicroscope will continue to work as smoothly and reliably as on the very first day.
We wish you the best of success in your work! after all, you are now equip­ped with the best tool!
Leica S Series User Manual 11
The Modular Design: Everything is possible
The Leica S series provides a high degree of flexibility in choosing equipment, thanks primarily to the modular configuration and the compatibility that Leica has painstakingly maintained for decades. The optics carriers, eyepieces, stands and more can be combined in any way you choose, allowing you to create the stereomicroscope that best suits your needs.
Despite this, you will notice that the controls and individual components do not differ signi­ficantly. Whichever configuration you choose, you will quickly feel right at home with your new stereomicroscope.
Have a special request? Let us know!
Leica Microsystems enjoys an exceptional repu­tation when it comes to devising customer­specific solutions. If you have a special request that cannot be met with standard parts, contact your Leica consultant. He or she has a solution for every application.
Leica S Series User Manual 12
What your stereomicroscope has to offer you
The optical system of the Leica StereoZoom® line consists of two beam paths converging at 12°. The objective pairs of each optical path are positioned close together, so the stereomicro­scopes can be of very "slender" design, espe­cially towards the base of the instrument. The advantage: The advantages of this design are that it has a small space requirement for use on bonders and in machine applications, unobst­ructed access to specimens, plenty of space for tools and a completely clear view of the object field.
The Greenough system enables cost-effective correction of aberrations such as chromasia, image field curvature, and distortion with mini­mal effort. In the new Leica StereoZoom® line, the optimum corrected center of the objec­tive is used for the image. This provides supe­rior optical performance with large, level and undistorted fields of view and chromatically optimized, high-contrast images.
ESD protection
The Leica S4 E, S6 E, S6, S6 D and S8 APO, including its cold light source and stand, is made from highly conductive material with surface resistivity of 2-10 11 Ohm/square, with a discharge time of <2 seconds, 1000 V to 100 V.
The Leica S6 T terminator version for highly complicated working ranges and the T incident light stand consist of a dissipative material with a surface resistivity of 102-106 Ohm/square and a discharge time of <0.1 seconds from 1000 V to zero.
Photography
The StereoZoom® models Leica S6 D and S8 APO are equipped with an integrated video/ phototube, which allows the simple, fast moun­ting of digital cameras
Apochromatic correction
The Leica S8 APO is a completely apochromati­cally corrected Greenough system. The modern optical technology of the StereoZoom® Leica S8 APO corrects chromatic aberration perfectly, removes bothersome color seams and displays pin-sharp images of even the finest enlarged detail. Contrast, brilliance, image sharpness, resolution, color fidelity and image precision are unsurpassed. The benefit of apochromatic correction is best seen in specimens that have a fine, low-contrast structure such as large animal cells, cilia plants or metallic microelectronic structures.
The technical features of the individual models are found on page 52.
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The model series
Leica S4 E
Leica S6 T
Leica S Series User Manual 14
Leica S6 E
Leica S6 D
Leica S6
Leica S8 APO
On We Go
If your new Leica stereomicroscope has already been assembled and commissioned by your Leica consultant, click here to skip through the installation instructions and go directly to the Quick Start Guide on Page 26.
If, on the other hand, you are assembling the stereomicroscope yourself, continue with the "Assembly" chapter, which begins on Page 16.
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Assembly
Leica S Series User Manual 16
Installing the basic equipment (overview)
6
5
4
3
2
1
Leica S Series User Manual 17
1. Sub-base for transmitted light with glass stage plate
2. Incident light base with stage plate
3. Focusing column with microscope carrier
4. Additional objective, optional
5. StereoZoom® optics carrier
6. Eyepieces, fixed and/or adjustable
Focusing column
Never unscrew the 3 screws on the right
•
side of the focusing column.
Focusing column on the incident light base
1. Remove the stage plate.
2. Route the 3 hollow screws from below through the base plate and screw these securely into the focusing column.
3. Insert the stage plate back into place.
Leica S Series User Manual 18
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