Living up to Life
User Manual
Leica S series
General Instructions
Safety concept
Before using your stereomicroscope for the first
time, please read the "Safety concept" brochure
included with your instrument. It contains
additional information about handling and
care.
Use in clean rooms
The Leica S series can be used in clean rooms
without any problems.
About cleaning
O Do not use any unsuitable cleaning agents,
chemicals or techniques for cleaning.
O Never use chemicals to clean colored
surfaces or accessories with rubberized
parts. This could damage the surfaces, and
specimens could be contaminated by abraded particles.
O In most cases, we can provide special solu-
tions on request. Some products can be
modified, and we can offer other accessories for use in clean rooms.
Servicing
O Repairs may only be carried out by Leica
Microsystems-trained service technicians.
Only original Leica Microsystems spare
parts may be used.
Responsibilities of person in charge of
instrument
O Ensure that the Leica stereomicroscope
is operated, maintained and repaired by
authorized and trained personnel only.
Leica S Series User Manual 2
Important Safety Notes
User Manual
The individual modules of the Leica stereomicroscope include an interactive CD-ROM with
all relevant user manuals in several languages.
Keep it in a safe place, and readily accessible
to the user. User manuals and updates are also
available for you to download and print from
our web site www.leica-microsystems.com.
This User Manual describes the special functions of the Leica StereoZoom® stereomicroscopes (S series) and contains important instructions for their operational safety,
maintenance, and accessories.
The "Safety Concept" booklet contains additional safety information regarding the service
work, requirements and the handling of the
stereomicroscope, accessories and electrical
accessories as well as general safety instructions.
You can combine individual system articles
with articles from external suppliers (e.g. cold
light sources, etc.). Please read the user manual
and the safety requirements of the supplier.
Before installing, operating or using the instruments, read the user manuals listed above. In
particular, please observe all safety instructions.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
Leica S Series User Manual 3
Symbols Used
Warning of a danger
This symbol indicates especially impor-
tant information that is mandatory to
read and observe.
Failure to comply can cause the following:
O Hazards to personnel
O Functional disturbances or damaged inst-
ruments
Warning of hazardous electrical voltage
This symbol indicates especially impor-
tant information that is mandatory to
read and observe.
Failure to comply can cause the following:
O Hazards to personnel
O Functional disturbances or damaged inst-
ruments
Danger due to hot surface.
This symbol warns against touching hot
surfaces, e.g. those of light bulbs.
Important information
This symbol indicates additional infor-
mation or explanations that intend to
provide clarity.
Explanatory notes
O This symbol within the text stands for addi-
tional information and explanations.
Figures
(1) Numbers in parentheses within the
descriptions relate to the figures and the
items within those figures.
Leica S Series User Manual 4
Safety Instructions
Description
The individual modules fulfill the highest requirements for observation and documentation of
Leica stereomicroscopes of the S series.
Intended use
O Refer to "Safety Concept" booklet
Non-intended use
O Refer to "Safety Concept" booklet
Never use S series microscopes or their components for surgical procedures (e.g. on the eye)
unless they are specifically intended for that
purpose.
The devices and accessories described in this
User Manual have been tested with regard to
potential hazards. The responsible Leica affiliate
must be consulted whenever the instrument is
altered, modified or used in conjunction with
non-Leica components that are outside of the
scope of this manual!
Unauthorized alterations to the instrument or
noncompliant use shall void all rights to any
warranty claims!
Place of use
O Refer to "Safety Concept" booklet
O Electrical components must be placed at
least 10 cm away from the wall and from
flammable substances.
O Avoid large temperature fluctuations,
direct sunlight and vibrations. These conditions can distort measurements and micrographic images.
O In warm and warm-damp climatic zones,
the individual components require special
care in order to prevent the build-up of
fungus.
Responsibilities of person in charge of
instrument
O Refer to "Safety Concept" booklet
Ensure that:
O The S series stereomicroscopes and acces-
sories are operated, maintained and
repaired by authorized and trained personnel only.
O All operators have read, understood and
observe this User Manual, and particularly
the safety instructions.
Leica S Series User Manual 5
Safety Instructions (continued)
Repairs, service work
O Refer to "Safety Concept" booklet
O Only original Leica Microsystems spare
parts may be used.
O Before opening the instruments, switch off
the power and unplug the power cable.
Touching the live circuit can cause
injury.
Transport
O Use the original packaging for shipping or
transporting the individual modules of the
Leica S stereomicroscopes and the accessory components.
O In order to prevent damage from vibra-
tions, all moving parts that (according to
the user manual) can be assembled and
disassembled by the customer should be
disassembled and packed separately.
Integration in third-party products
O Refer to "Safety Concept" booklet
Disposal
O Refer to "Safety Concept" booklet
Legal regulations
O Refer to "Safety Concept" booklet
EC Declaration of Conformity
O Refer to "Safety Concept" booklet
Leica S Series User Manual 6
Safety Instructions (continued)
Health risks
Workplaces with stereomicroscopes
•
facilitate and improve the viewing task,
but they also impose high demands on the eyes
and holding muscles of the user. Depending on
the duration of uninterrupted work, asthenopia
and musculoskeletal problems may occur. For
this reason, appropriate measures for reduction
of the workload must be taken:
O Optimal arrangement of workplace, work
assignments and work flow (changing tasks
frequently).
O Thorough training of the personnel, giving
consideration to ergonomic and organizational aspects.
O The ergonomic optical design and const-
ruction of the Leica S stereomicroscopes
are intended to reduce the exertion of the
user to a minimum.
Direct contact with eyepieces is a
•
potential transmission method for
bacterial and viral infections of the eye.
The risk can be kept to a minimum by using
personal eyepieces for each individual or
detachable eyecups.
Leica S Series User Manual 7
Table of Contents
General Instructions 2
Important Safety Notes 3
Symbols Used 4
Safety Instructions 5
Table of contents 8
Leica S Series
Congratulations! 11
The Modular Design: Everything is Relative 12
What your stereomicroscope has to offer you 13
The model series 14
On We Go 15
Assembly
Installing the basic equipment (overview) 17
Focusing column 18
Sub-base for transmitted light and cold light source 19
Optics carrier and additional objective 20
Available graticules 21
Inserting graticules 22
Eyepieces 23
Leica LED illuminators 24
Mounting a camera (Leica S6 D and S8 APO) 25
Quick Start Guide
A stereomicroscope of the S series in overview 27
Tips for working ergonomically 28
Using the Eyepieces 29
The correct interpupillary distance 30
Focusing 31
Switching magnification (zooming) 32
Limiting zoom range 33
Regulating the resistance of the focusing drive 35
Changing the position of the optics carrier 36
Diopter settings and parfocality: 1 adjustable & 1 fixed eyepiece 37
Dioptric correction with two adjustable eyepieces 40
Photography & Video
Photography & Video 44
Photography with the Leica S6 D and S8 APO 45
Dimensional drawings in mm
Leica S6 E (S4 E / S6 T) with incident light and transmitted light
illumination 47
Leica S6 48
Leica S6 D with incident light and transmitted light illumination 49
Leica S8 APO with incident light and transmitted light illumination 50
Leica S Series User Manual 8
Specifications
Overview of technical features 52
Specifications 53
Appendix
Calculating the total magnification/field of view diameter 55
Troubleshooting 56
Care, Maintenance, Contact Persons 57
Leica S Series User Manual 9
Leica S series
Leica S Series User Manual 10
Congratulations!
Congratulations on obtaining your new Leica StereoZoom® line stereomicroscope (S series). We are convinced it will exceed your expectations, as
this instrument embodies all the qualities you associate with the name
Leica Microsystems: excellent objectives, high-quality engineering, and
reliability. Furthermore, the modular design ensures that the Leica stereomicroscope adapts perfectly to your needs – no matter which accessories
you require for your tasks.
Thanks to the parfocal system with simultaneously large working distances and object fields, you can always view your microscopic specimens
accurately – from the complete image to the finest detail.
Though the reliability and robustness of Leica stereomicroscopes is worldrenowned, like any high-tech product, the Leica S series requires a certain
degree of care and attention. Therefore, we recommend that you read
this manual. It contains all the information you need regarding operation,
safety and maintenance. Simply observing a few guidelines will ensure
that even after years of intensive use, your stereomicroscope will continue
to work as smoothly and reliably as on the very first day.
We wish you the best of success in your work! after all, you are now equipped with the best tool!
Leica S Series User Manual 11
The Modular Design: Everything is possible
The Leica S series provides a high degree of
flexibility in choosing equipment, thanks
primarily to the modular configuration and
the compatibility that Leica has painstakingly
maintained for decades. The optics carriers,
eyepieces, stands and more can be combined in
any way you choose, allowing you to create the
stereomicroscope that best suits your needs.
Despite this, you will notice that the controls
and individual components do not differ significantly. Whichever configuration you choose,
you will quickly feel right at home with your
new stereomicroscope.
Have a special request? Let us know!
Leica Microsystems enjoys an exceptional reputation when it comes to devising customerspecific solutions. If you have a special request
that cannot be met with standard parts, contact
your Leica consultant. He or she has a solution
for every application.
Leica S Series User Manual 12
What your stereomicroscope has to offer you
The optical system of the Leica StereoZoom®
line consists of two beam paths converging at
12°. The objective pairs of each optical path are
positioned close together, so the stereomicroscopes can be of very "slender" design, especially towards the base of the instrument. The
advantage: The advantages of this design are
that it has a small space requirement for use on
bonders and in machine applications, unobstructed access to specimens, plenty of space for
tools and a completely clear view of the object
field.
The Greenough system enables cost-effective
correction of aberrations such as chromasia,
image field curvature, and distortion with minimal effort. In the new Leica StereoZoom® line,
the optimum corrected center of the objective is used for the image. This provides superior optical performance with large, level and
undistorted fields of view and chromatically
optimized, high-contrast images.
ESD protection
The Leica S4 E, S6 E, S6, S6 D and S8 APO,
including its cold light source and stand, is
made from highly conductive material with
surface resistivity of 2-10 11 Ohm/square, with a
discharge time of <2 seconds, 1000 V to 100 V.
The Leica S6 T terminator version for highly
complicated working ranges and the T incident
light stand consist of a dissipative material with
a surface resistivity of 102-106 Ohm/square and
a discharge time of <0.1 seconds from 1000 V
to zero.
Photography
The StereoZoom® models Leica S6 D and S8
APO are equipped with an integrated video/
phototube, which allows the simple, fast mounting of digital cameras
Apochromatic correction
The Leica S8 APO is a completely apochromatically corrected Greenough system. The modern
optical technology of the StereoZoom® Leica
S8 APO corrects chromatic aberration perfectly,
removes bothersome color seams and displays
pin-sharp images of even the finest enlarged
detail. Contrast, brilliance, image sharpness,
resolution, color fidelity and image precision
are unsurpassed. The benefit of apochromatic
correction is best seen in specimens that have a
fine, low-contrast structure such as large animal
cells, cilia plants or metallic microelectronic
structures.
The technical features of the individual models
are found on page 52.
Leica S Series User Manual 13
The model series
Leica S4 E
Leica S6 T
Leica S Series User Manual 14
Leica S6 E
Leica S6 D
Leica S6
Leica S8 APO
On We Go
If your new Leica stereomicroscope has already
been assembled and commissioned by your
Leica consultant, click here to skip through the
installation instructions and go directly to the
Quick Start Guide on Page 26.
If, on the other hand, you are assembling the
stereomicroscope yourself, continue with
the "Assembly" chapter, which begins on
Page 16.
Leica S Series User Manual 15
Assembly
Leica S Series User Manual 16
Installing the basic equipment (overview)
6
5
4
3
2
1
Leica S Series User Manual 17
1. Sub-base for transmitted light with glass stage plate
2. Incident light base with stage plate
3. Focusing column with microscope carrier
4. Additional objective, optional
5. StereoZoom® optics carrier
6. Eyepieces, fixed and/or adjustable
Focusing column
Never unscrew the 3 screws on the right
•
side of the focusing column.
Focusing column on the incident light base
1. Remove the stage plate.
2. Route the 3 hollow screws from below
through the base plate and screw these
securely into the focusing column.
3. Insert the stage plate back into place.
Leica S Series User Manual 18
Sub-base for transmitted light and cold light source
1. Remove the glass stage plate.
2. Pull the locking mechanism forward.
3. Set the incident-light stand to the sub-base
for transmitted light and engage it with the
connection screw.
4. Press the locking mechanism backward.
The incident light base and the sub-base
for transmitted light are now connected.
5. Insert the glass stage plate.
6. Insert the universal light guide in the
opening at the rear.
Additional information can be found in
the User Manual for cold-light source
Leica KL300 LED.
Leica S Series User Manual 19
Optics carrier and additional objective
Optics carrier
1. Insert the optics carrier carefully in the
microscope carrier and fasten it in the desired position with the clamping screw.
Additional objective (optional)
1. Screw the desired objective counterclockwise into the optics carrier.
Protective objective glass (optional)
1. Screw the objective protective glass directly
onto the StereoZoom® or on the additional
objective.
Leica S Series User Manual 20
Available Graticules
The optional graticules enable measure-
ment and, in addition, provide valuable
information when comparing and capturing
still images of the specimens. Insert the graticule before you set the eyepiece in place.
Available Graticules
The following graticules and objective micrometers for calibrating may be ordered:
O Graticule 10 mm/0.1 mm
O Graticule 5 mm/0.1 mm
O Graticule 5 mm/0.05 mm
O Graticule 100 Div./0.002"
O Graticule 100 Div./0.001"
O Graticule 150 Div./0.0005"
O Crosshairs
O Stage micrometer 50 mm, 0.1/0.01/ mm
graduation
O Stage micrometer 1", 0.001" graduation
Leica S Series User Manual 21
Inserting graticules
Graticules can be inserted in the adjus-
table eyepieces and the in the eyepieces
for eyeglass wearers.
The procedure for taking measurements
is described in the "Measuring" user
manual.
Inserting graticule(s)
1. Use the stereomicroscope to determine the
side on which the scale is inscribed. The
scale must not appear reversed.
2. Remove the insert from the bottom of the
eyepiece and place it on the bench with the
knurled side down.
3. Hold the graticule by the edges to avoid
leaving fingerprints, and push it into the
holder from the side.
4. Replace the insert in the eyepiece and press
it firmly into place.
5. Insert the eyepiece in the tube and turn the
eyepiece in the tube to align the graticule
correctly.
Leica S Series User Manual 22
Eyepieces
You can use your Leica StereoZoom®
together with a fixed or adjustable eyepiece. For models in which a graticule is included
in an eyepiece for measurement or photography, two eyepieces are necessary. We recommend that you also equip the high-powered
StereoZoom® Leica S8 APO with two adjustable
eyepieces.
Inserting the eyepieces
1. Push eyepieces as far as they will go into
the tubes.
2. Check that the eyepieces are seated firmly
and precisely in place.
Risk of infection
Direct contact with eyepieces is a poten-
tial transmission method for bacterial
and viral infections of the eye. The risk can be
kept to a minimum by using personal eyepieces
for each individual or detachable eyecups.
Leica S Series User Manual 23
Leica LED illumination
The Leica KL300 LED cold light source
with its fiber-optic light guides is ideally
suited for the Leica S4 E, S6 E, S6 and S6 T
stereomicroscopes. A number of matching
adapters are available for connecting the
Leica KL300 LED cold light source to various
stereomicroscope stands and for stand-alone
operation.
For detailed information about installation and
use, please refer to the user manual for the
Leica KL300 LED.
Please note that the universal light guide
on the Leica S8 APO can only be used
with the light arm mounted on the side.
High-output illuminators
For higher demands, such as photogra-
•
phy or in use combination with the Leica
S8 APO, we offer different high-performance
transmitted light stands and LED incident light
illumination, as in the Leica LED3000 series,
for example. Please ask your Leica consultant
about the options.
Leica S Series User Manual 24
Camera design (Leica S6 D and S8 APO)
The Leica S6 D and S8 APO are equipped
with an integrated video/phototube,
which allows the simple, fast mounting of digital cameras for photos and video. Please ask
your Leica consultant about the options.
Detailed information about the availa-
ble camera systems, accessories and the
software packages from Leica can be found in
the corresponding manuals.
Installing the camera
1. Remove the protective dust cover from the
video/projection lens (C-mount adapter)
and the microscope camera.
2. Screw the camera into the video/projection
lens (C-mount).
3. Insert the unit into the video/photo output
of the stereomicroscope and screw it in.
Always close the video/photo output
•
with the protective dust cover if no
camera is installed.
Leica S Series User Manual 25
Quick Start Guide
Leica S Series User Manual 26
An overview of an S series stereomicroscope
1 Magnification changer, right drive knob with
magnification scale
2 S6/S8 models: Stop for zoom limit
3 Focusing drive
4 Fixing screw for optics carrier in the microscope
carrier
5 Adjustable tube: Interpupillary distance
adjustable from 55 to 75 mm
6 Eyepieces
7 Threads for fastening the light arm
(both sides and rear)
8 Illumination Leica LED3000 SLI
9 Illumination Leica LED3000 RL
10 Leica microscope camera
11 C-mount adapter and video/photo tube
10
11
6
3
7
5
1
2
4
8
9
Leica S Series User Manual 27
Tips for working ergonomically
Align your stereomicroscope optimally.
You must configure the settings described here precisely in order to be able to take
full advantage of its outstanding optical and
ergonomic advantages.
O Align your workstation optimally. Consider
the height of the bench and chair.
O Use the whole seat surface and the back-
rest.
O Ensure that your lower arms are supported.
O When carrying out other tasks, perform
exercises to relax and relieve muscle
tension.
Ergonomic objectives
The ErgoObjective for the Leica S4 E and all
S6 models provides for fatigue-free work. The
ErgoObjective 0.6 – 0.75× with an adjustable
working distance of 77 – 137 mm and the ErgoObjective 0.7 – 1.0× with an adjustable working
distance of 48 – 98 mm allow the fine adjustment of the working distance, the magnification and the viewing level without time-consuming changing of lenses.
The Leica S6, with a 60° viewing angle, offers
optimum viewing height on the inclined
stereomicroscope.
Leica S Series User Manual 28
Using the Eyepieces
The eyepieces form the connection
between the tube and the eye of the
observer. Simply push them into the tube and
they are ready to use.
What does "parfocal" mean?
"Parfocal" means that a specimen conti-
nues to remain exactly in focus, even
if the magnification on the stereomicroscope
is modified. All stereomicroscopes from Leica
Microsystems are parfocally matched. However, the parfocality requires a personal dioptric
correction for the user.
Dioptric Correction
In order to parfocally match the stereomicroscope, at least one eyepiece with dioptric
correction is necessary. The setup is described
on the following pages:
O With one adjustable and one fixed eyepi-
ece: from page 37.
O With two adjustable eyepieces: from
page 40.
If you do not wear glasses:
Depending on the preferences of the observer,
eyecups can be used.
To avoid eye infections, we recommend
that every user uses his or her own pair
of eyecups.
If you wear glasses:
Eyeglass wearers must remove or fold
back the eyecups (Fig. below left), as
otherwise they cannot see the entire field of
view.
Leica S Series User Manual 29
The correct interpupillary distance
The interpupillary distance is correctly
set if you see a single circular image field
when looking at a specimen.
If you are still a novice microscope user, you
may need a short time to become accustomed
to this. Not to worry - after a little while, it will
become automatic.
Reference value
The interpupillary distance can be set between
55 and 75 mm.
An "exit pupil distance" is the distance
between eye and eyepiece. With the
10×/23B wide-field eyepiece for eyeglass
wearers, it is approx. 22 mm. For those who do
not use the eyepiece for eyeglass wearers, it is
12 mm.
Setting the eye distance
1. Bring the eyes slowly to the eyepieces.
2. Push the tubes together or apart with both
hands until you see a single round, circular
image field without shadows with both
eyes.
Leica S Series User Manual 30
Focusing
Focusing raises and lowers the stereomi-
croscope using the focusing drive. The
specimen detail is brought into sharp focus as
soon as it is in the focal point of the objective.
The focusing drive can be operated
either left- or right-handed.
Focusing
1. Align the specimen under the objective.
2. Set the lowest magnification level.
At the lowest magnification, the desired
specimen detail can be easier to localize
due to the large field of view.
3. Look into the eyepieces and insert the desired specimen detail in the center.
4. Focus on the specimen with the drive knob.
Leica S Series User Manual 31
Changing magnification (zooming)
All stereomicroscopes of the S series
allow a continuous magnification
change. The magnification changer can be
operated with the left and the right hand. The
image scale is shown on the right drive knob.
The basis for the calculation of the total
magnification and the field of view can
be found on page 26.
Changing magnification
1. Look into the eyepieces.
2. Focus on the object (see page 16).
3. Rotate the magnification changer until the
desired magnification is configured.
Leica S Series User Manual 32
Limiting zoom range
With the S6 models and the S8 APO, the zoom
range can be limited at the top and bottom. In
the same manner, a fixed magnification level
can be set. The following example shows the
limit to the range between 1 to 3.2.
Defining the lower limit
1. Loosen the hollow screw on the left drive
knob with the Allen key provided.
2. Turn the right drive knob to position "1".
3. Set the stop on the left drive knob forward
until it touches the built-in zoom stop.
4. Carefully tighten the Allen screw.
Continued on next page.
Leica S Series User Manual 33
Limiting zoom range (continued)
Defining the upper limit stop
1. Loosen the hollow screw on the right drive
knob with the Allen key provided.
2. Turn the right drive knob to position "3.2".
3. Set the stop backward on the right drive
knob until it touches the built-in zoom
stop.
4. Carefully tighten the allen screw.
Leica S Series User Manual 34
Regulating the resistance of the focusing drive
Adjusting the resistance
Is the focusing drive too loose or too tight? Does
the equipment tend to slide downwards? The
resistance can be adjusted individually depending on the equipment weight and personal
preferences as follows:
1. Grip the outer drive knobs with both hands
and turn them towards each other until the
desired resistance is reached during focusing.
Leica S Series User Manual 35
Changing the position of the optics carrier
The optics carrier can be turned side-
ways in the microscope carrier if the user
wants to work from the side.
Changing position
1. Unscrew the clamping screw.
2. Turn the optics carrier laterally to the desired position.
3. Carefully tighten the clamping screw.
Leica S Series User Manual 36
Diopter settings and parfocality: 1 adjustable & 1 fixed eyepiece
If you set the diopters on the adjustable
eyepiece exactly as described, the image
will remain equally sharp and constant (parfocal) from the lowest to the highest magnification. This means you do not have to refocus
when changing magnification. The focus needs
to be readjusted only if you want to view a
specimen detail that is located higher or lower.
Use this advantage as often as possible, it is not
available on all stereomicroscopes.
The diopters can be set between +5
and -5.
The following adjustments have to be
carried out only once by each user. Using
reticules leads to slightly deviated settings,
which are described in the user manuals on the
reticules (measurement).
Adjusting the diopter settings
1. With Leica S6 D and Leica S8 APO, turn the
rotary knob to the "Vis" setting.
2. Turn the dioptric correction on the adjustable eyepiece to the center position.
Continued on next page
Leica S Series User Manual 37
Diopter settings and parfocality: 1 adjustable & 1 fixed eyepiece (continued)
3. Place a flat specimen under the objective.
4. Set the lowest magnification level.
5. Observe the specimen through the eyepieces and bring it into sharp focus with the
focusing drive.
6. Set the highest magnification level.
7. Optimize the focusing with the focusing
drive.
8. Set the lowest magnification level.
9. Turn the eyelens of the eyepiece as far as it
will go in the "+" direction, without looking
into the eyepieces while doing so.
10. Close your eye on the fixed eyepiece and
look with the other eye into the into the
adjustable eyepiece.
11. Rotate the eyelens of the eyepiece slowly in
the "–" direction until the eye can see the
specimen sharply.
Continued on next page
Leica S Series User Manual 38
Diopter settings and parfocality: 1 adjustable & 1 fixed eyepiece (continued)
Checking parfocality
1. Select the highest magnification level.
2. Monitor the specimen; if necessary, refocus
it slightly.
3. Change from the highest to the lowest
magnification. The sharpness should be
constant (parfocal). If this is not the case,
repeat this procedure.
Leica S Series User Manual 39
Dioptric correction with two adjustable eyepieces
If you set the diopters on the adjustable
eyepiece exactly as described, the image
will remain equally sharp and constant (parfocal) from the lowest to the highest magnification. This means you do not have to refocus
when changing magnification. The focus needs
to be readjusted only if you want to view a
specimen detail that is located higher or lower.
Use this advantage as often as possible, it is not
available on all stereomicroscopes.
The diopters can be set between +5
and -5.
The following adjustments have to be
carried out only once by each user. Using
reticules leads to slightly deviated settings,
which are described in the user manuals on the
reticules (measurement).
Adjusting the diopter settings
1. With Leica S6 D and Leica S8 APO, turn the
rotary knob to the "Vis" setting.
2. Turn the dioptric correction on both eyepieces to the center position.
Continued on next page
Leica S Series User Manual 40
Dioptric correction with two adjustable eyepieces (continued)
3. Place a flat specimen under the objective.
4. Set the lowest magnification level.
5. Observe the specimen through the eyepieces and bring it into sharp focus with the
focusing drive.
6. Set the highest magnification level.
7. Optimize the focusing with the focusing
drive.
8. Set the lowest magnification level.
9. Turn the eyelens of the eyepiece as far as it
will go in the "+" direction, without looking
into the eyepieces while doing so.
10. Look through the eyepieces and close one
eye.
11. With the other eye, monitor the specimen
and turn the eyelens of the eyepiece slowly
in the "–" direction, until this eye sees the
specimen sharply.
12. Repeat steps 10 and 11 with the other eye.
Continued on next page
Leica S Series User Manual 41
Dioptric correction with two adjustable eyepieces (continued)
Checking parfocality
1. Select the highest magnification level.
2. Monitor the specimen; if necessary, refocus
it slightly.
3. Change from the highest to the lowest
magnification. The sharpness should be
constant (parfocal). If this is not the case,
repeat this procedure.
Leica S Series User Manual 42
Photography & Video
Leica S Series User Manual 43
Photography & Video
For most stereomicroscope users, digital documentation has become an invaluable part of
their work. Research results can be presented in
an appealing form, while measurements on the
digital image provide clarity.
Adapters
If camera control using the Leica Application
Suite is not required, conventional mirror reflex
and rangefinder cameras from third-party
manufacturers can be used. Leica Microsystems
offers various adapters for these.
Leica DFC cameras
If you require absolute control over the camera
and need the capability for measurement,
evaluation and more in addition to photography, the digital Leica DFC cameras are exactly
right for you. Together with the Leica Application Suite, they provide virtually limitless freedom of use. For additional information about
Leica cameras, refer to the camera's documentation.
Leica Application Suite
The "Leica Application Suite", or "LAS" for short,
is, as it were, the digital extension of your
stereomicroscope. With it, you can not only take
images, but also control the illumination, the
camera and more. For additional information,
refer to the LAS online help.
Leica S Series User Manual 44
Photography with the Leica S6 D and S8 APO
The observation beam path and the
photobeam path can be switched. For
this, the light splitting is influenced as follows:
O Position "Vis": 100 % light in both eyepie-
ces, but no light in the video/photo beam
path
O Position "Doc": 100 % light in the right
eyepiece, but no light in the left eyepiece.
100 % of the light crosses the video/photo
beam path
The focusing and the framing are done
with the left eyepiece (video/photo
beam path).
Flat specimens are sometimes partially
blurry on the left and right edge of the
image. This blurring is based on laws of optics
and does not mean there is a malfunction of the
camera or the microscope.
Capturing images and videos
1. If the image detail and image sharpness are
set to your satisfaction, switch on the "Doc"
setting and capture your image.
Leica S Series User Manual 45
Dimensional drawings in mm
Leica S Series User Manual 46
Leica S6 E (S4 E / S6 T) with incident light and transmitted light illumination
144
161
ø58
ø76
46
223
107
278
22
38
351
65
25
81110
46
38
351
81110
22
161
25
ø58
ø76
107
278
223
104
113
148
205
278
205
144
104
113
148
205
220
278
205
Leica S Series User Manual 47
Leica S6
60
390
81
110
144
46
120
25
25
ø58
ø76
26
148
ø15.8
35
104
150.4
Leica S Series User Manual 48
Leica S6 D with incident light and transmitted light illumination
351
38
22
144
207
107
25
81110
351
38
22
65
278
ø58
ø76
144
104
113
148
205
220
278
205
171
207
171
223
25
81110
ø58
ø76
107
278
104
113
148
205
278
205
Leica S Series User Manual 49
Leica S8 APO with incident light and transmitted light illumination
351
22
144
22
38
11675
207
171
25
ø58
ø76
107
278
205
104
113
148
205
278
38
351
65
11675
207
25
ø58
ø76
107
278
171
223
144
104
113
148
205
220
278
205
Leica S Series User Manual 50
Technical data
Leica S Series User Manual 51
Overview of technical features
StereoZoom® Zoom Enlargement Viewing angle Extras
Leica S4 E 4.8:1 6.3×-30× 38° ErgoObjective™
Leica S6 E 6.3:1 6.3×-40× 38° ErgoObjective™
Leica S6 6.3:1 6.3×-40× 60° ErgoObjective™
Leica S6 T 6.3:1 6.3×-40× 38° Terminator
Leica S6 D 6.3:1 6.3×-40× 38°
Leica S8 APO 8:1 10×-80× 38°
StereoZoom® is a trademark registered in the Principal Register of the US Patent and Trademark Office.
ErgoObjective™
Video photo tube
ErgoObjective™
Apochromatic Greenough system
Apochromatic zoom
Apochromatic objectives
Video photo tube
Leica S Series User Manual 52
Technical data
®
StereoZoom
Optical system,
lead-free
Zoom 4.8:1 6.3:1 6.3:1 6.3:1 6.3:1 8:1, apochromatic
Viewing angle 38° 60° 38° 38° 38° 38°
ESD protection antistatic antistatic antistatic Terminator (dissipating) antistatic antistatic
Specific surface
resistivity
Magnification (basic configuration)
Maximum resolution 372 lp / mm 432 lp / mm 432 lp / mm 432 lp / mm 432 lp / mm 600 lp / mm
Maximum num. About the
Aperture
Working distance (basic
config.)
Object field diameter 36.5 mm 36.5 mm 36.5 mm 36.5 mm 36.5 mm 23 mm
Adjustable zoom limits 2 2 2 2 2
Video/photo outlet,
switchable
Image acquisition, coax lighting. Yes Yes
Standard objectives, lead-free Achromats
ErgoObjective
Adjustable Objectives 0.3× – 0.4× / 200–350 mm 0.3× – 0.4× / 200 – 350 mm 0.3× – 0.4× / 200–350 mm 0.3× – 0.4× / 200 – 350 mm 0.3× – 0.4× / 200 – 350 mm
Ergonomic eyepieces, fixed
and adjustable, with cups
Ergonomic eyepieces for eyeglass
wearers, adjustable, with eyecups
Interpupillary distance 55 – 75 mm 55 – 75 mm 55 – 75 mm 55 – 75 mm 55 – 75 mm 55 – 75 mm
™
Leica S4 E Leica S6 Leica S6 E Leica S6 T Leica S6 D Leica S8 APO
12°-Greenough usingbestcorrected central part of
the objective
2 ×1011 Ω / square,
discharge time
<2 seconds from
1000 V to 100 V
6.3× – 30× 6.3× – 40× 6.3× – 40× 6.3× – 40× 6.3× – 40× 10× – 80×
0.124 0.144 0.144 0.144 0.144 0.2
110 mm 110 mm 110 mm 110 mm 110 mm 75 mm
0.32×, 0.5×,0.63×,
0.75×, 1.6×, 2.0×
0.6× – 0.75× / 77 – 137 mm
0.7× – 1.0× / 48 – 98 mm
10× / 23, 16× / 16, 20× / 12 10× / 23, 16× / 16, 20× / 12 10× / 23, 16× / 16, 20× / 12 10× / 23, 16× / 16, 20× / 12 10× / 23, 16× / 16, 20× / 12
10× / 23, 16× / 15,
25× / 9.5, 40×/ 6
12°-Greenough usingbestcorrected central part of
the objective
2 ×1011 Ω / square,
discharge time
<2 seconds from
1000 V to 100 V
Achromats
0.32×, 0.5×,0.63×,
0.75×, 1.6×, 2.0×
0.6× – 0.75× / 77 – 137 mm
0.7× – 1.0× / 48 – 98 mm
10× / 23, 16× / 15,
25× / 9.5, 40×/ 6
12°-Greenough usingbestcorrected central part of
the objective
2 ×1011 Ω / square,
discharge time
<2 seconds from
1000 V to 100 V
Achromats
0.32×, 0.5×,0.63×,
0.75×, 1.6×, 2.0×
0.6×–0.75× / 77 – 137 mm
0.7× – 1.0× / 48 – 98 mm
10× / 23, 16× / 15,
25× / 9.5, 40×/ 6
12°-Greenough usingbestcorrected central part of the
objective
102–106 Ω / square,
discharge time
< 0.1 seconds from 1000 V
tozero
Achromats
0.32×, 0.5×,0.63×,
0.75×, 1.6×, 2.0×
0.6×–0.75× / 77 – 137 mm
0.7×–1.0× / 48 – 98 mm
10× / 23, 16× / 15,
25× / 9.5, 40×/ 6
12°-Greenough usingbestcorrected central part of
the objective
2 ×1011 Ω / square, discharge time
<2 seconds from
1000 V to 100 V
100 % visual or 100 %
video/photo and 100 %
visual in the left eyepiece
Achromats
0.32×, 0.5×,0.63×,
0.75×, 1.6×, 2.0×
0.6×–0.75× / 77 – 137 mm
0.7×–1.0× / 48 – 98 mm
10× / 23, 16× / 15,
25× / 9.5, 40×/ 6
12°-Greenough usingbestcorrected central part of
the objective
2 ×1011 Ω / square,
discharge time
<2 seconds
from 1000 V to100 V
100 % visual or 100 % video/photo and 100 %
visual in the left eyepiece
Apochromats 0.63×, 1.6×, 2.0×
Achromat 0.32×
10× / 23, 16× / 15,
25× / 9.5, 40×/ 6
Leica S Series User Manual 53
Appendix
Leica S Series User Manual 54
Calculating the total magnification/field of view diameter
Parameter
M
Magnification of the additional objective
O
M
Magnification of eyepiece
E
z Magnification changer position
N
Field number of the eyepiece. Field numbers are printed on
FOV
the eyepieces: 10×/23, 16×/16, 20×/12, 10×/23B, 16×14B,
25×/9.5B, 40×6B.
Magnification in the binocular tube
M
TOT VIS = MO × ME × z
or
1.6 × 20 × 4 = 128
Example
M
M
z Zoom position 4.0
Calculation example: Field of view diameter in the specimen
Additional objective 1.6×
O
20×/12 eyepiece
E
∅
OF:
N
FOV
MO × z 1.6 ×4
12
=
= 1.9 mm
Leica S Series User Manual 55
Troubleshooting
The field of view is shadowed
O Adjusting the correct Interpupillary
Distance (P. 52).
The image goes out of focus.
O Inserting the eyepieces correctly (P. 55).
O Perform diopter correction exactly accor-
ding to the instructions (from P. 31).
The focusing drive gradually sinks on its
own or is difficult to turn.
O Regulate the ease of movement (P. 30).
In the case of failures of electrically operated devices, always first check:
O Is the voltage selector set correctly?
O Is the main power switch switched on?
O Is the power cable connected correctly?
O Are all connecting cables attached
correctly?
O Are the fuses intact?
Photos are blurry.
O Focus accurately (P. 23).
O Bring the reticule into sharp focus and
perform diopter correction exactly according to the instructions (P. 37).
O Insert the eyepieces correctly up to the stop
(P. 35).
O Check that the graticules are securely in
place in the eyepiece (P. 31).
The image from the camera stays black
O Switch the beam splitter on the photo tube
to the "Doc" setting (P. 37).
Leica S Series User Manual 56
Care, Maintenance, Contact Persons
We hope you enjoy using your stereomicroscope. Leica devices are renowned for their
robustness and long service life. Observing the
following care and cleaning tips will ensure that
even after years and decades, your Leica stereomicroscope will continue to work as well as it
did on the very first day.
Warranty benefits
The guarantee covers all faults in materials
and manufacture. It does not, however, cover
damage resulting from careless or improper
handling.
Contact address
However, if your instrument should no longer
function properly, contact your technician,
your Leica representative or Leica Microsystems
(Schweiz) AG, CH-9435 Heerbrugg.
E-mail contact:
stereo.service@leica-microsystems.com
Care
O Protect your stereomicroscope from mois-
ture, fumes and acids and from alkaline,
caustic and corrosive materials and keep
chemicals away from the instruments.
O Plugs, optical systems and mechanical
parts must not be disassembled or replaced, unless doing so is specifically permitted and described in this manual.
O Protect your stereomicroscope from oil and
grease.
O Do not grease guide surfaces or mechanical
parts.
Leica S Series User Manual 57
Care, Maintenance, Contact Persons (continued)
Protection from dirt
Dust and dirt will affect the quality of your
results.
O Put a dust cover over the stereomicroscope
when it will not be used for a long time.
O Use dust caps to protect tube openings,
tubes without eyepieces, and eyepieces.
O Keep accessories in a dust-free place when
not in use.
Cleaning polymer components
Some components are made of polymer or are
polymer-coated. They are, therefore, pleasant
and convenient to handle. The use of unsuitable cleaning agents and techniques can
damage polymers.
Permitted measures
O Clean the stereomicroscope (or parts of it)
using warm soapy water, then wipe using
distilled water.
O For stubborn dirt, you can also use ethanol
(industrial alcohol) or isopropanol. When
doing so, follow the corresponding safety
regulations.
O Remove dust with a pneumatic rubber bulb
or with a soft brush.
O Clean objectives and eyepieces with special
optic cleaning cloths and with pure alcohol.
Leica S Series User Manual 58
The productive cooperative effort "with the user, for the user" has always been the
basis for the innovative strength of Leica Microsystems. On this, we have developed
our five corporate values:
pioneering, high-end quality, team spirit, dedication to science, and continuous
improvement. We call making these values reality Living up to Life.
INDUSTRY DIVISION
The Leica Microsystems Industry Division's focus is to support customers' pursuit of
the highest quality end result by providing the best and most innovative imaging
systems for their needs to see, measure, and analyze microstructures. Its solutions
are used in routine and research industrial applications, in materials science and
quality control, in forensic science investigations, and educational applications.
Leica Microsystems – an international company with an experienced worldwide
customer service network.
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10ID S10060E N ∙ Copyright © by Leic a Microsystems (Schweiz) AG, CH-9 435 Heerbru gg, 2013.
Subject to modif ications. LEICA and the Leica Lo go are registered tr ademarks of
Leica Microsyste ms IR GmbH.
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