Leica NCL-L-CD7-580 User Manual

™

Novocastra

Liquid
Mouse Monoclonal Antibody
CD7

Product Code: NCL-L-CD7-580

Intended Use

Specicity

Clone

Ig Class

Antigen Used for
Immunizations

Hybridoma Partner

Preparation

Effective on Frozen Tissue

Effective on Parafn Wax
Embedded Tissue

Recommendations on Use

Positive Controls

Staining Pattern

Storage and Stability

Warnings and Precautions

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Human CD7 antigen.

LP15

IgG2b

Prokaryotic recombinant fusion protein corresponding to a portion of the extracellular domain of
the human CD7 molecule.

Mouse myeloma (p3-NS1-Ag4-1).

Liquid tissue culture supernatant containing sodium azide.
Volume as indicated on vial label.

Not tested.

Yes

Immunohistochemistry on parafn sections.

Heat Induced Epitope Retrieval (HIER): Please follow the instructions for use in Novocastra
Epitope Retrieval Solution pH 6.

Suggested dilution: 1:100 for 30 minutes at 25 °C. This is provided as a guide and users should
determine their own optimal working dilutions.

Visualization: Please follow the instructions for use in the Novolink™ Polymer Detection Systems.

For further product information or support, contact your local distributor or regional ofce of Leica

Biosystems, or alternatively, visit the Leica Biosystems web site, www.LeicaBiosystems.com
The performance of this antibody should be validated when utilized with other manual staining
systems or automated platforms.

Western Blotting: Not recommended.

Immunohistochemistry: Tonsil.

Membrane

Store at 2–8 °C. Do not freeze. Return to 2–8 °C immediately after use. Do not use after

expiration date indicated on the vial label. Storage conditions other than those specied above
must be veried by the user.

This reagent has been prepared from the supernatant of cell culture. As it is a biological product,
reasonable care should be taken when handling it.
This reagent contains sodium azide. A Material Safety Data Sheet is available upon request or
availa ble from www.LeicaBiosystems.com

Leica Biosystems Newcastle Ltd
Balliol Business Park West
Benton Lane
Newcastle Upon Tyne NE12 8EW
United Kingdom
( [phone]

www.LeicaBiosystems.com

CD7-580-L 10/06/2013

© Leica Biosystems Newcastle Ltd. • NCL-L Rev D

General Overview

General References

The CD7 molecule is a membrane-bound glycoprotein of 40 kD and is the earliest T cell specic

antigen to be expressed in lymphocytes. CD7 antigen is also the only early marker to persist

throughout differentiation. The function and role of the CD7 molecule has not yet been identied,

although the activation of T cells with gamma/delta receptors has been proposed based on
mAb-induced activation. CD7 antigen is reported to be found on the majority of peripheral blood T
cells, most natural killer cells and thymocytes.

Leong FJW-M and Leong AS-Y. The Journal of Histotechnology. 2002; 25(4):215-227.
Ormsby A, Bergfeld WF, Tubbs RR et al. Journal of the American Academy of Dermatology. 2001;
45(3):405-413.

www.LeicaBiosystems.com

© Leica Biosystems Newcastle Ltd • NCL-L Rev D