Leica NCL-L-CD33 User Manual

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Novocastra

Liquid Mouse Monoclonal Antibody CD33

Product Code: NCL-L-CD33

Intended Use

Specicity

Clone

Ig Class

Antigen Used for Immunizations

Hybridoma Partner

Preparation

Effective on Frozen Tissue

Effective on Parafn Wax Embedded Tissue

Recommendations on Use

Positive Controls

Staining Pattern

Storage and Stability

Warnings and Precautions

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Human CD33 antigen, both isoforms; CD33m (31 kD) and CD33M (67 kD).

PWS44

IgG2b

Prokaryotic recombinant protein corresponding to a region of the C2 domain of the human CD33 molecule.

Mouse myeloma (p3-NS1-Ag4-1).

Liquid tissue culture supernatant containing sodium azide. Volume as indicated on vial label.

Not evaluated.

Yes

Immunohistochemistry on parafn sections.

Heat Induced Epitope Retrieval (HIER): Please follow the instructions for use in Novocastra Epitope Retrieval Solution pH 9.

Suggested dilution: 1:100 for 30 minutes at 25 °C. This is provided as a guide and users should determine their own optimal working dilutions.

Visualization: Please follow the instructions for use in the Novolink

For further product information or support, contact your local distributor or regional ofce of Leica

Biosystems, or alternatively, visit the Leica Biosystems web site, www.LeicaBiosystems.com The performance of this antibody should be validated when utilized with other manual staining systems or automated platforms.

Western Blotting: Typical working dilution 1:100–1:500.

Immunohistochemistry: Bone marrow or granulocytic sarcoma.

Western Blotting: U937 cell line.

Cytoplasmic and Membrane.

Store at 2–8 °C. Do not freeze. Return to 2–8 °C immediately after use. Do not use after

expiration date indicated on the vial label. Storage conditions other than those specied above must be veried by the user.

This reagent has been prepared from the supernatant of cell culture. As it is a biological product, reasonable care should be taken when handling it. This reagent contains sodium azide. A Material Safety Data Sheet is available upon request or availa ble from www.LeicaBiosystems.com

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Polymer Detection Systems.

Leica Biosystems Newcastle Ltd Balliol Business Park West Benton Lane Newcastle Upon Tyne NE12 8EW United Kingdom ( +44 191 215 4242

www.LeicaBiosystems.com

CD33-L 13/05/2014

General Overview

General References

The CD33 molecule is the smallest member of the structurally related group of IgSF domaincontaining sialic acid binding proteins called the sialoadhesin family. CD33 antigen is reported to be absent from pluripotential stem cells but appears on the myelomonocytic precursors after CD34 antigen. It then continues to be expressed on both the myeloid and monocyte lineages, although it is reported to be absent on granulocytes.

Bradshaw EM, Chibnik LB, Keenan BT, et al. Nature Neuroscience. 2013, 16(7): 848 – 852. Hoyer JD, Grogg KL, Hanson CA, et al. American Journal of Clinical Pathology. 2008; 129(2): 316 – 323.

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