Leica NCL-CK17 User Manual
NovocastraTM Lyophilized
Mouse Monoclonal Antibody
Cytokeratin 17
Product Code: NCL-CK17
Intended Use
Specicity
Clone
Ig Class
Antigen Used for
Immunizations
Hybridoma Partner
Preparation
Effective on Frozen Tissue
Effective on Parafn Wax
Embedded Tissue
Recommendations on Use
Positive Controls
Staining Pattern
Storage and Stability
Warnings and Precautions
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Human cytokeratin 17 intermediate lament protein.
E3
IgG2b
Keratin preparation from rat enterocytes.
Mouse myeloma (X63-Ag8.653).
Lyophilized tissue culture supernatant containing sodium azide.
Reconstitute with the volume of sterile distilled water indicated on the vial label.
Yes
Yes
Immunohistochemistry on parafn sections.
Heat Induced Epitope Retrieval (HIER): Please follow the instructions for use in Novocastra
Epitope Retrieval Solution pH6.
Suggested dilution: 1:20 for 30 minutes at 25 °C. This is provided as a guide and users should
determine their own optimal working dilutions.
Visualization: Please follow the instructions for use in the Novolink™ Polymer Detection Systems.
For further product information or support, contact your local distributor or regional ofce of Leica
Biosystems, or alternatively, visit the Leica Biosystems Web site, www.LeicaBiosystems.com
The performance of this antibody should be validated when utilized with other manual staining
systems or automated platforms.
Immunohistochemistry: Trachea.
Western Blotting: HeLa cell line.
Cytoplasmic.
Store unopened antibody at 2–8 °C. Under these conditions, there is no signicant loss in product
performance up to the expiry date indicated on the vial label. Do not use after expiration date
indicated on the vial label. The reconstituted antibody is stable for at least two months when
stored at 2–8 °C. For long term storage, it is recommended that aliquots of the reconstituted
antibody are stored frozen at -20 °C (frost-free freezers are not recommended). Repeated
freezing and thawing must be avoided. Prepare working dilutions on the day of use. Return to 2–8
°C immediately after use. Storage conditions other than those specied above must be veried
by the user.
This reagent has been prepared from the supernatant of cell culture conrm. As it is a biological
product, reasonable care should be taken when handling it. This reagent contains sodium azide.
A Material Safety Data Sheet is available upon request or available from
www.LeicaBiosystems.com
Leica Biosystems Newcastle Ltd
Balliol Business Park West
Benton Lane
Newcastle Upon Tyne NE12 8EW
United Kingdom
( +44 191 215 4242
© Leica Biosystems Newcastle Ltd • NCL- Rev D CK17 21/01/2014
General Overview
General References
NCL-CK17 reacts with the human cytokeratin intermediate lament protein (46 kD) identied
as cytokeratin 17. In normal tissues, NCL-CK17 is reported to react mainly with basal cells of
complex epithelia; basal cells of pseudostratied epithelium in the trachea, larynx and bronchi.
The antibody also reportedly reacts with myoepithelial cells in salivary glands and sweat glands.
Markey A C, Lane E B, MacDonald D M, et al.. British Journal of Dermatology. 126: 154–160
(1992).
Troyanovsky S M, Guelstein V I, Tchipysheva T A, et al.. Journal of Cell Science. 93: 419–426
(1989).
Guelstein V I, Tchipysheva T A, Ermilova V D, et al.. International Journal of Cancer.42: 147–153
(1988).
www.LeicaBiosystems.com
© Leica Biosystems Newcastle Ltd • NCL- Rev D
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