Leica NCL-CEA-2 User Manual
NovocastraTM Lyophilized
Mouse Monoclonal Antibody
Carcinoembryonic Antigen (CD66e)
Product Code: NCL-CEA-2
Intended Use
Specicity
Clone
Ig Class
Antigen Used for
Immunizations
Hybridoma Partner
Preparation
Effective on Frozen Tissue
Effective on Parafn Wax
Embedded Tissue
Recommendations on Use
Positive Controls
Staining Pattern
Warnings and Precautions
Storage and Stability
Leica Biosystems Newcastle Ltd
Balliol Business Park West
Benton Lane
Newcastle Upon Tyne NE12 8EW
United Kingdom
( +44 191 215 4242
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Human carcinoembryonic antigen (CD66e).
12-140-10
IgG1
CEA isolated from liver metastasis of colorectal carcinomas by PCA extraction followed by ion
exchange and gel ltration chromatography.
Mouse myeloma (X63-Ag8).
Lyophilized tissue culture supernatant containing sodium azide.
Reconstitute with the volume of sterile distilled water indicated on the vial label.
Yes
Yes
Immunohistochemistry on parafn sections.
Enzyme Induced Epitope Retrieval (EIER): Please follow the instructions for use in Novocastra
Enzyme Proteinase K (IHC).
Suggested dilution: 1:200 for 30 minutes at 25 °C. This is provided as a guide and users should
determine their own optimal working dilutions.
Visualization: Please follow the instructions for use in the Novolink™ Polymer Detection Systems.
For further product information or support, contact your local distributor or regional ofce of Leica
Biosystems, or alternatively, visit the Leica Biosystems Web site, www.LeicaBiosystems.com
The performance of this antibody should be validated when utilized with other manual staining
systems or automated platforms.
Immunohistochemistry: Colon.
Western Blotting: Not evaluated.
Cytoplasmic and lumenal membrane.
This reagent has been prepared from the supernatant of cell culture conrm. As it is a biological
product, reasonable care should be taken when handling it. This reagent contains sodium azide.
A Material Safety Data Sheet is available upon request or available from
www.LeicaBiosystems.com
Store unopened antibody at 2–8 °C. Under these conditions, there is no signicant loss in product
performance up to the expiry date indicated on the vial label. Do not use after expiration date
indicated on the vial label. The reconstituted antibody is stable for at least two months when
stored at 2–8 °C. For long term storage, it is recommended that aliquots of the reconstituted
antibody are stored frozen at -20 °C (frost-free freezers are not recommended). Repeated
freezing and thawing must be avoided. Prepare working dilutions on the day of use. Return to
2–8 °C immediately after use. Storage conditions other than those specied above must be
veried by the user.
© Leica Biosystems Newcastle Ltd • NCL- Rev D CEA-2 12/01/2014
General Overview
General References
Carcinoembryonic antigen (CD66e) is a heterogeneous cell surface glycoprotein produced by
cells of fetal colon. Low levels are also found on normal mucosal epithelia of the adult colon
and a variety of other normal tissues. CD66e is encoded by the CEA gene that is located on
chromosome 19. It is a member of the CEA gene family, which in turn is a subfamily of the
immunoglobulin superfamily. Cell adhesion properties are now well recognized for CD66e. It
is believed that the expression of this glycoprotein in conjunction with other known adhesion
molecules will inuence cell-cell interaction.
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Börmer O P. Journal of Immunological Methods. 121:85–93 (1989).
Roitt I, et al.. Immunology Second Edition 1989.
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Patz M, Odermatt B, Christen B, et al.. Virchows Archiv A–Pathological Anatomy and
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www.LeicaBiosystems.com
© Leica Biosystems Newcastle Ltd • NCL- Rev D CEA-2 12/01/2014
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