Leica MS5, MZ75, MZ6, MZ12s, MZ16 User Manual

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Leica
M Ster(•o-
microscO})(~s
U
ser Manual
MICROSYS
TEMS
D•·ar
User
User Manual
Thank
you
for
choosing
our
products. We
hope
that
you
will
en-
joy
the
quality
and
performance
of Lei
ca
Microsystems
products
.
In
developing
our
instr
uments,
we
have
placed
great
emphasis
on
simple,
self-explanatory directions.
In
order
to util
ize
all
the
benefits
of
your
new stereomicroscope,
we
suggest
studying
this
user
manual in det
ail.
Should
you
have
any
questions,
please
consult
your
local
Leica
representative.
You
will
find
the
address
of
the
closest local representati
ve
as
well
as
valuable
informa-
tion
about
products
and
services
from
Leica
Microsystems
on
our
homepage
at
www.leica-microsystems.
com
We
are
gladly
at
your
service.
Customer
service
is a big
thing
with us.
Not
only
before
the
sale,
but
afterwards
as
well.
Leica
Microsystems (Switzerland)
Ltd
Business
unit Stereomicroscopy
www.stereomicroscopy.
com
Your
instrument
is
accompanied
by a printed
English
user
man-
ual.
Additional
language
versions
and
information
can
be
found
on
the
interactive
CD-ROM. User
manuals
and
updates
are
availab
le for download
on
our
homepage
at
www.stereomicroscopy.
com
.
This
user
manual
contains
an
explanation
of
the
safety regula-
tions,
assembly,
handling
and
accessories
of
the
Leica
MS5,
MZ6,
MZ75,
MZ9
s,
MZ12
s,
MZ16
and
MZ16
A (if identical) stereo-
microscopes.
The
special functions
of
the
automated
stereo-
microscope
Leica
MZ16 A can
be
found
in
the
separate
user
manual
M2-116-0
.
Leica
M Stereomicroscopes
User
Manual
3
Table
of
eontent
s
Page
Overview
Illuminators
Safety conce
pt
...................................... ..
..
l:i
Transformers
.............
.....................
.. .
......
38
Symbols
.......................
.. . .
...............
......
9
Incident
lamp 6 V/10 W ........
....
...........
........... 40
Controls
and functions .... . . . .
....
...
................ .
..
10
Incident
lamp 6 V/20
W .. .
.............................. .
42
Cold
light
sources
...................................... 45
Use
Fiber-optic light
guide
.. . .
.................
.....
.. .
...... 46
Changing
the
magnification
........
......................
12
LED
illumination .... .
.............
....
................
..
46
Ergonomics .....
..........
............
...
....
.. .....
...
14
Supply
(line)
lamp
25
W ...............
.........
.........
47
Interpupilla
ry
distance
........
.. ..
................
.. .
...
15
Coaxial ill
uminator
.. ..................................
..
47
Eye
point ...... . ..
................. . .....
....
.. . . ..
.... 15
Near
-vertical illuminator
....... . .
......
.. ..... ..
.. ..
...
..
48
Eye
contact,
eyecups .................. .....
...
.......
..
16
Focusing ...................
...........................
16
Accessories
Microscope carrier: Stereoscopic
and
axial
observati
on .... 18
Fitting
accessory
tubes
......
.......
.. .. .
.... .......
...
..
50
Diopte
r settings
........................................
20
Double-iris
diaphragm
.................
.....
......
...... 50
Drawing
tube
, di
scussion
tube
.. . . . .
....
..............
.. .
51
Assembly
Video/phototubes:
Overview: Components
.. ......
.....
.. .. .
...
.. ......
.. .
..
22
Photography,
video,
TV,
filming, digital
imaging ...
.. .
....
...
52
Attachment for vertical
and
oblique
observation
..........
..
56
Stand
Graticules
.............
.. .
.........
...
...............
..
56
Profile
column
for
incident light
and
transmitted
-light
stands
..
23
Stages, pol
arization ............
....
.........
.. . . . .
...
.. .
57
Transmitted-li
ght
stand,
bright
field,
20
W ..
................
24
Transmitted-li
ght stand,
bright
and
dark
field
...
....
.. . . .
...
26
Special
notes
Transm
itted-li
ght
stand
HL
..................
.............
27
Tips
and
hints: What
to
do
if ....
.......................
...
58
Swinging-arm
stand
ESD
...... ..
.......
.. . ...... . . .
.....
28
Care
and
maintenance
...
.. .
............
.. .. .........
...
59
Large
swinging-arm a
nd
table-c
lamp
stands
.........
......
30
Calculating tot
al
magnification
and
field
of
view
diameter
.. ..... . .
......................
....
.. ..
61
Optics
carrier,
optical
accessories
Optical
data
for
MS5,
MZ6
.. . . . .................
.. .
...
...
62
Spacer
rings,
objective combinations ..
...
....
. .. .....
.... 32
Optical
data
for
MZ7
5,
MZ95
.. . ......
.....
............. ..
64
Microscope carri
er
...
.. . .
............
....
.. .
........... 34
Optical
data
for
MZ125, MZ16, MZ16
A .. ..... .
..........
..
66
Optics
carrier
...
. .. . .
.................................. 34
Dimensions ......
.. ...................
.......
.. ........
67
Binocular t
ubes
, optical accessories
.....
.. ....
....
...
....
35
Objective nos
epie
ce
(MZ16
and
MZ16
A)
... . . .........
....
36
4
Leica M Stereomicroscope
s Us
er
Manu
al
Leica M Stereomicroscopes
User
Manual
5
6
General directions Read
the
user
manual
and
safety
instructions
before
starting up.
Permitted uses
The
Leica MS5,
MZ6,
MZ7
s, MZ9
s,
MZ12
s.
MZ16
and MZ16 A stereomi cro-
scopes are
optical instruments which
use
magnification for improved
visual
ization
of objects, their details
and
specimens from technology and
natural sciences.
The
basic outfit, which inc ludes a stand and a light
source, can
be
supplemented with vario
us
modules for photography, TV,
dual
station viewing
and
other applications.
Prohibited
• The use of the instrument in a different manner from
that desc
ribed
in
uses this user manual can lead to injury, malfunction
and
damage.
• The Leica MS5, MZ6, MZ7s, MZ9s,
MZ12
s,
MZ16
and
MZ16 A stereo-
microscopes must not
be
used for
eye
examinations
and
operations.
Do
not fit different plugs.
Do
not dismantle optical systems or mechani -
cal parts unless instructions for doing
so
are given
in
the user manual.
Place •
The Leica
MS5, MZ6,
MZ7s, MZ9s, MZ12
s.
MZ16
and
MZ16 A stereo-
of use microscopes are intended
ma
inly for indoor
use
.
If
the stereomicroscope
is
used outdoors, protect
it
from dirt
and
mois-
ture. Lamps
and
electrically-operated stands from Leica may not
be
used outdoors.
Use in
ESD
The
optics carriers
MS5
, MZ6, MZ7s, MZ9s, MZ12s, MZ16
and
MZ16
A,
the
protected rooms
binocular tube, Ergo
Tube™,
ErgoWedge™ 5°-25°,
the swinging-arm
stand
ESD
and
the cold light sources Leica
L2
and
CLS
consist of
ESD­discharging material (surface resistance <10" ohms/squared, discharge t1me
<2 seconds,
1,000
V to
100
V).
Servicing
Responsibilities
of person in charge
of instrument
- A connection socket for a 0 4
mm
grounding cable
is
provided
on
the
microscope carrier.
- There are
two
connection sockets for 0 4
mm
grounding cables
in
the
base of the swinging-arm stand.
Repairs may
on
ly
be
carried out by Leica-trained service technicians.
Only original Leica spare parts may
be
used.
• Ensure that personnel who use the instrument have read
and
under-
stood this user
manual and in particular the safety instructi
ons.
• Ensure
thatthe
Leica MS5, MZ6,
MZ7
s, MZ9s,
MZ12s
, MZ16
and
MZ16
A
stereomicroscopes are operated, maintained
and
repaired only
by
authorized
and
trained personnel.
Leica
M Stereomicroscopes -
Safety
concept
Safety
rf'g;ulations
Workplaces with stereomicroscopes facilitate
and
improve the
viewing task, but they
also impose high demands
on
the eyes
and
holding muscles of the user. Dependent upon the duration
of uninterrupted activity, this may
lead to asthenic trouble
and
muscular-skeletal pain
so
that
suitable measures must
be
taken
to reduce the strain:
- perfect design of workplace (see p.
14).
work content and
work process (frequently changing activities).
- detailed introduction of the personnel while considering ergonomic
and
organizational work-related aspects.
The
ergonomic optics concept
and
the design
ofthe
Leica M
series
aim
at limiting the strain
on
the user to the lowest possi-
ble level.
Direct contact with eyepieces can
be
a potential transmission
path for
bacterial
and
viral infections of the
eye.
Users should
be
made aware of the potential risk of infection.
The
risk can
be
kept at a minimum
by
using individual eyepieces or attachable
eyecups (see
p.16)
.
Electrically-operated
items
The safety regulations listed below apply to the following items:
- Transmitted-light stand, bright field,
20
W (page
24)
- Supply (line) lamp
25
W (page
47)
- Regulating transformer 0- 7
V/40
W (page
38)
- Step transformer 4/
5/6
V (page
39)
-
Motor
focus (separate instructions)
- Motori
zed
Leica
MZ16
A
Liquids
Be
careful when handling liquids.
If spilt
on
the equipment, they can :
- cause the stereomicroscopes
and
accessories to become
electrically live, endangering personnel,
-
cause damage to the equipment.
Power
cables Inspect cables regularly for damage. Defective cables can:
- directly endanger personnel,
-
cause the stereomicroscopes
and
accessories to become
electrically live, endangering personnel.
Leica
M Stereomicroscopes -
Safety
regulations
7
Cable
routing
Opening
the
instrument
Supply
voltage
Connections
Route
cables
with
care.
Make
sure
that
personnel
do
not
get
entangled
with
them. The
instrument
could
fall
over,
fall
down,
become
damaged,
damage
other
equipment,
or
cause
personal
m
Jury
.
Only
authorized
Leica
personnel
are
permitted
to
repair electri-
cal
equipment.
Before
opening
up
the
equipment, pull
out
the
power
cable.
If
you
touch
the
live
interior
of
the
equipment, you
may
receive
an
electric
shock.
Ensure
that
the vol
tage
setting
on
the
equipment
is
correct.
A
wrong
setting
can
cause
damage
to
the
equipment.
When
connecting
power-consuming
devices
to
the
transform-
ers,
do
not
exceed
the
maximum
permitted
loading,
as
this
can
cause
damage
to
the
equipment.
Changing
bulbs • Before
changing a bulb,
disconnectthe
power
cable.
8
Integration
in
third-party
products
legal
requirements
Disposal
Conformity
with
European
Community
directive
Never
attempt
to
touch
the
inside
of a lamp
housing
or
transmitted-light
stand
while
the
instrument
is
connected
to
the
power
supply
.
Before
changing a bulb,
wait until it
is
cool,
otherwise
you
may
burn
your
fingers.
The
following
must
be
observed
if
Leica
products
are
build
into
third-
party
products:
The
manufacturer
of
the
complete
system
or
the
person
putting
it
on
the
market
is
responsible
for
adhering
to
applicable
safety
regulations,
laws
and
guidelines
.
Adhere
to
general
and
local
regulations
relating
to
accident
prevention
and
environmental
protection.
The
products
described
here
must
be
disposed
off
in
accor-
dance
with applicable local
laws
and
regulations.
The
electrically-
operated
accessories
for
the
Leica
MS5,
MZ6,
MZ7
s,
MZ9
s,
MZ12
s,
MZ16
and
MZ16
A stereomicroscopes
are
constructed
in
accordance with
the
latest technologies
and
provided
with a
statement
of
conformity with
EC
requirements.
Leica M Stereomicroscopes-Safety regu
lations
Symbols
used
in
this
user
manual
Safety
instructions
This
symbol
denotes
information which
must
be
read
and
obeyed.
Non-observance
-
can
put
personnel
at
risk!
-
can
cause
malfunctioning
or
damage
to
the
equipment.
Warning
about
accessible
hot
places,
e.g.
bulbs.
Failure
to
respect
it
can
cause
burns!
Important
information
This
symbol
denotes
additional information
or
explanations
which
promote
understanding
.
Action
..,..
This
symbol
within
the
text indicates that certain
operations
must
be
carried
out.
Explanatory
notes
This
symbol
within
the
text
stands
for additional information
and
explanations
.
Leica M Stereomi croscope s - Safety regulations
9
Overview
Stereomicroscope
Magnification
changer
2
Engaging
ratchet
positions
3a
Focusing
drive
3b
Coarse
and
fine
focusing
Larger
knob:
Coarse
focusin
g,
Smaller
knob:
Fine
focusi
ng.
3c
Adjustable
ring
Changes
the
ease
of
movement
of
the
coarse/fine
drive.
4
Clamping
screw
Holds
the
optics carrier
in
the
microscope
carrier.
5
Clamping
screw
Holds
the
binocular
tube (or
accessory)
to
the optics carrier.
6
Interchangeable
objective
Achromatic, planachromatic
or
planapochromatic
Leica
MS5
Binocular
tube
7
Adjustable
eyepiece
tubes
Interpupillary distance
adju
stab
le from
52
mm
-76
mm.
8
Wide-field
eyepieces
for
persons
wearing
glasses
Diopter
setting
adjustable within
the
range
fr
om
+5
to
-5,
and
eye
cups
adjustable.
9
Clamping
screws
These
hold
the
eyepieces
in
the
binocular
tube.
10
ErgoTube™
The
viewing
angle
is
adjustable within the
r
ange 10°-50°.
11
Built-in
double-iris
diaphragm
(MZ16/MZ16
A
only)
The
special
functions
of
the
Leica
MZ16
A
are
shown
in
the
user
manual
M2-116-0.
Leica
MZ6
10
Leica M Stereomicroscopes-Overview
Leica
MZ7s
Leica
MZ9s
Leica design
by
Erne
st
lgi/Christophe Apotheloz
Leica
MZ12s
Leica
MZ16
Leica
M Stereomicroscopes -
Overview
11
Use
12
Changing
the
magnification
Ratchet
steps
Magnification
changer
Zoom
means
continuous
change
of
magnification.
Ratchets
can
be
engaged
for certain magnifications
(see
below).
For
zooming
with
MZ16
A,
see
the
user
manual
M2-116-0.
MS5: 5 steps MZ6
: 6:1
zoom
MZ7
s:
7.9:1
zoom
MZ9
s: 9.
5:1
zoom
MZ12
s: 12.5:1
zoom
MZ16
and
MZ16
A:
16:1
zoom
The
magnification
changer
can
be
operated
from
either
the
right
or
the
left
side
.
~Look
into
the
eyepieces.
~Focus
on
the
object (
page
16).
~Turn
the magnification
changer
to
the
desired
position
.
MZ6:
0.8,
1,
1.25
, 1.
6,
2, 2.
5,
3.2
MZ7
s: 0.8,
1,
1.25,
1.6,
2, 2.5, 3.2,
4
MZ9
s:
0.8,
1, 1.
25,
1.6, 2, 2.5, 3.
2,
4,
5
MZ12
s:
1,
1.25, 1.6, 2,
2.5, 3.2,
4,
5,
6.3, B
MZ16
: 0.
8,
1,
1.25, 1.
6,
2, 2.
5, 3.2,
4,
5, 6.3,
B,
10
Engage
the
ratchets whenever magnifications
need
to
be
exactly reproducible.
~For
MZ6, MZ9
s,
MZ12s and
MZ16:
Press
the
switch.
~For
MZ7
s:
Turn
the knurled
ring
counterclockwise.
For
setting ratchets
at
the
MZ16
A,
see
the
user manual
M2-116-0.
Lei
ca M Stereomicroscopes -
Oper
ation
Scale
of
magnifications
Magnifications
and
fields
of
view
Magnification
with
MZ12s/MZ16
planachromatic
and
planapochromatic
objectives
MS5,
MZ6:
Display
of
magnification factors
from 0.63-4
.
MZ7s:
Display
of
magnification factors
from 0.63-5
.
Corresponding
scales
for other objective
and
eyepiece
combi-
nations
can
be
affixed
to
the
rotary
knobs
(page
34).
MZ9s:
Display
of
magnification factors
from 0.63-6.
MZ12s:
Display
of
magnification factors
from
0.8-10.
MZ16,
MZ16
A:
Display
of
magnification factors
from 0.71-11
.5
MZ16
A:
For
the
digital display
of
the magnification
see
the
user
manual
M2-116-0
.
The
tables
on
pages 62-
66
provide information
about
the
magnifications
and
field of view
diameters
as
a function
of
the
magnification
changer
position
of
the
eyepiece-objective
combination
used
.
When
the
MZ12s/MZ16
planachromatic
and
planapochromatic
objectives
are
used
on
the
Leica
MS5,
MZ6,
MZ7s and
MZ9
s,
the
magnification
is
increased
by
the
factor
1.25
x.
This
factor
is
taken
into
account
in
the
tables.
Magnification
on
Magnification
on
MZ12s/MZ16
MS5/MZ6/MZ7s/MZ9
s
1x
plan/planapo
1.25x
1.6x
planapo
2x
0.63x
planapo
O.Bx
0.5x plan
0.63x
0.8x
plan
1x
2x
planapo
2.5x
Leica
M Stereomicroscopes - Operation
13
14
Preconditions
fm
working
comfortably
Ergonomics,
viewing
height
Pay
great attention when setting
up
you
r stereomicroscope.
You will
only benefit fully from the outstanding optical
and
ergonomic advantages of the instrument if
you
set it
up
precisely
in
accordance with
the
directions already
given.
For
fatigue-free work,
use
the ErgoModule™.
Arrange your workplace
as
well
as
possible,
and
pay
attention
to
the heights of chair
and
table.
• When sitting,
use
the entire seat
area
and
also
the
back
support of the chair.
• Support your forearms.
• During work
breaks,
stand
up
and
move
arou
nd for exercise.
Please
also
observe
the
note
on
p.
7.
Ergonomics
The
reasons why
users
often cannot work comfortably with
the 45° tube
are
the differing heights
of
the outfits, the various
accessories
and
working distances,
and
the fact that
the
same
ins
trument
is
often
used
by
several
people
of
differing
builds.
This
problem
can
be
effectively
solved
by
using
the
Leica
ErgoModule™.
Viewing
height,
viewing
angle
If
you
have
problems with
the
viewing configuration
on
your
stereomicroscope,
ask
your
Leica
consultant for the most
ergonomic
Leica solutions.
The
viewing height
is
correct when your
head
and
back
are
comfortably positioned.
On
the
Ergo
Tube™,
the viewing
angle
is
adjustable from 10°-50°.
lliJ>
Holding
the
eyepiece
tubes
at
their
bases,
swi
ng
them
up
or
down
(see
arrow).
.
.,
,,~0
'
"
~
10°
Lei
ca M
Stereomi
crosco
pes-Op
era
tion
ErgoModule™
-
ErgoWedge™
5°-25°
-
ErgoWedge™±15
°
- ErgoModule™
50
mm
-
The
Ergo
Tube™
45°
has
eyepiece tubes wh
ich
are
65
mm
longer than the standard
ones.
Magnification factor 1.6
x.
MZ16
A:
The
magnificati
on
factor
can
be
toggled
on
and
off
using
the
OPT
key.
- ErgoModule™ 30-
120
mm
Relubrication
may
be
required
after several years (please
send
the
item
to Lei
ca
Service).
Ergo
objective
The
achromatic
Ergo
objective
0.4
x-0.63x
can
be
used
to
ergonomical
ly
and
accurately focus
in
the
range
of
90
mm
(63.5-153
.5
mm)
without changing
the
viewing height. At the
same
time, magnification
and
working distance
can
be
changed without time-consuming change of objectives.
Motor
focus
Frequently repeated fine-motor tasks,
such
as
focusing
by
hand, can
lead
to
muscle
fatigue
and
hand
problems. Usi
ng
the
Leica
motor focusing
system,
every equipment
can
be
moved
up
and
down without applying force.
Interpupillary
distance,
eye
contact
Interpupillary
distance
The
interpupillary distance
is
correct
when
you
see
a single circular field when
viewi
ng with
both
eyes.
~
76mm
~
lliJ>
Look
into
the
eyepieces
and
use both
hands
to
move
the
eyepiece tub
es
closer
together
or
further apart.
Locating
the
exit
pupil
The
distance between
eye
and
eyepiece measures approx.
22
mm
for 10x
/21
B wide-field eyepieces for persons we a
ring
glasses
and
12
mm
for 10x
/21
wide-fi
eld
eyepieces.
your
eyes until
you
can
see
the
complete
~
image
field without corner cutting.
lliJ>
Slowly approach
the
eyepieces with
!I~ ~."'
'f:?
"'l'
Yf
Leic
a M Stereom
icroscopes -
Operation
15
16
Selecting
the
degree
of
eye
contact
Soft
eyecups
You
do not wear glasses
and
prefer close contact with 1 Ox
/21
B
wide-field eyepieces for persons wea ring glasses:
~Holding
the diopter ring, turn the
eye
cup
counterclockwise
until it is released .
~Pull
the eyecup upwards .
~Holding
the diopter ring,
turn the
eye
cup clockwi
se.
Persons wearing glasses need the
whole of the available distance.
Move the
eye
cups to the lowest
position.
The
1 Dx/21
B wide-field
eye
pieces for persons we a ring glasses
are supplied with soft
eye
cups, whi
ch
can
be
attached to the in-
tegrated
eye
cups made of hard plastic to achieve the following:
- Prevent eye infections if several users are working at the same instrument (see
p.
7).
- Protect your glasses from being scratched.
The
10X/21
wide-field eyepieces are supplied with laterally
tapered soft eyecups.
Setting
the
working
distance,
focusing
=obtaining
a
sharp
image
To
focus,
use
the focusing drive to rai
se
or lower the stere-
omicroscope
until the feature of interest is
in
focus = working
distance to the objective.
The working distances
ofthe
various objectives are listed
in
the t a bles
on
pages
62-
66
.
• The focusing drive can
be
operated from either the right
or
the left side.
• When focusmg
on
a new object, always start with the lowest
magnification, because the
larger field of view mak
es
the
o
bj
ect
easier to find. Afterwards, select the desired magnifi-
cation.
Le
ic a M Stereomic rosc opes -
Ope
ration
Coarse/fine
drive
Adjusting
the
free
mning
of
the
focusing
drive
Focusing
drive
MS5-
MZ9s
Focusing
drive,
coarse/fine
MS5-
MZ12s
Focusing
drive,
coarse/fine
MZ16,
MZ16A
~Place
the object beneath the objective.
~Engage
the lowest magnification
(page
12).
~Look
into the eyepieces.
~Using
the rotary knob, bring the
object into focus.
~Focus
approximately with the
larger knob.
~
Fine-focus with the smaller (concentric) knob.
Is
the focus movement too loose or too tight? Does the outfit
tend to
slide downwards?
You
can adjust the movement
in
accordance with the weight of the outfit
and
with your own
personal requirements:
~Hold
the drive knobs firmly
wit
h
both hands
and
turn them
in opposite directions until you obtain the required degree of resistance during focusing.
~Turn
the black ring
on
the
right-hand drive knob
until you obtain the required degree of resistance duri
ng
focusing.
~Hold
the left fine drive.
~Turn
the right fine drive until the desired resistance during
focusing
is
reached.
Leica M Stereomicroscopes - Operation
17
18
Microscope carrier
for
stereoscopic
observation
Stereoscopic
observation
Microscope carrier
This
microscope carrier permits
only
stereoscopic observation, using
both
beam
paths.
The
microscope carrier
can
be fitted
to the drive
housing in either of two
posi
tions
(see
page
34)
:
The
optics carri
er
can
be
turned sideways
in
the microscope carrier if t
he
user
needs
to
work from the si
de:
..,. Rel
ease
the clamping screw.
..,.
Turn
the
optics carri
er
sideways
as
required.
..,.
Retighten
the clamping
screw.
Microscope carrier
AX
The
stereomicroscopes
can
be
equipped with t
he
microscope
carrier
AX for
stereoscopic
and
axial
observation instead of
the standa rd
mic
roscope carrie r
(page
34)
.
Different
mode
ls
are
available
for
MS5,
MZ6,
MZ7
s,
MZ9s and
for
MZ12s,
MZ16
and
MZ16
A.
The
achromatic objectives
and
the planochromatic
lx
fit
in
the thr
ead
in
the
AX
microscope
carrier for
MS5, MZ6,
MZ7s,
MZ9s
(p.
33).
The
planochromatic
and
planapochromatic objectives fit
in
the
thread
in
the
AX
microscope carrier for
MZ12s, MZ1
6,
MZ16
A
(p.
33).
In
addition, the
AX
microscope carri
er
features a clamp-
ing
screw for fixing it
in
one
of
3 positions
and
an
aperture which
can
be
turned
manually
(see
below).
Otherwise,
the
use
of
and
switchi
ng
to
the
left,
middle
and
right position
are
identical.
The
opti
cs
carrier
cannot
be
turned sideways
in
the
micro-
scope carrier
AX
.
A three-dimensional
image
is
required for
examining
plastic
objects
and
for working.
..,.
Shift the stereomicroscope to the central position.
Le1c
a M Stereomicroscopes -
Ope
ration
Axial
observation
Aperture
The
parallax-free i
maging
produces
more
precise
results
in
photogr
aphy,
measuring
and
polarization.
..,.
Shift the stereomicroscope to the left or the right positi
on.
The
object
is
now
located directly beneath either
the
left
or
the
right
beam
path
.
• When photographing or measuring,
se-
lectthe
beam
path
above
which
the
pho-
totube
or
measuring graticule
is
located .
A rotating aperture is
build
into the
AX
microscope carri
er.
lt
prevents interfering reflecti
ons
during
axial
observation with
coaxial incident illuminator
and
quarter-wave plate.
With the
AX
microscope carrier for
MS5,
MZ6,
MZ7s, MZ9s,
the aperture automatically turns during
the
moving
process.
• With
the
AX
microscope carrier for
MZ12s,
MZ16,
MZ16
A,
the
aperture
is
located underneath
the
microscope carrier
and
must manually be turned
as
follows:
Please
note:
-
In
the position for stereoscopic observa-
ti
on
through
both
beam
paths, turn
the
aperture counterclockwise until it
stops.
Otherwise
the
field
of
view
is
underex-
posed
(vignetting).
-
In
the
pos
ition for
axial
observation
through
the
right or left
beam
path, turn
the
aperture clockwise until it
stops.
Leica M Stereomicroscopes - IJperation
19
20
Tips
for
your
work
[I]
A Preparations
Adjusting diopter settings and parfocali1y
The
dioptr
ic
range
is
from
+5
to
-5.
The foll
owing
adjustments
must
be
carried
out only
once
by
each
user.
The
procedures for adjusting
the
dioptric
setting
and
the
parfocality
are
somewhat differ
ent
if
an
eyepiece
contains
a graticule.
Refer
to
the
user
manuals
for
the
MPS
modular
photomicrographic
systems
or
for
measuring.
~Install
the
lamp
.
~Set
the
interpupillary distance
and,
if
necessary,
the
viewing
height.
~On
the
video/phototube:
Move
the
l
ever
to
position "VIS" and
open
the
diaphragm.
~Set
the
double
-iris di
aphragm
to
"OPEN."
~Adjust
the
eyecups.
~Set
both
eyepieces
to
zero.
~Set
the
microscope carrier
AX
for
stereoscopic
observation.
B
Focus
on
the
1.
Place
a flat test object
under
the
test object objecti
ve.
2.
Engage
the
lowest magnification
position.
~Use
the
coarse
focusing
drive
to
set
the
working di
stance
(page
16)
.
3.
Look into
the
eyepieces
~Use
the
foc
using
drive, to
bring
the test object into
foc
us.
leica
M Stereomicrosco
pes-Operat
ion
C Adjusting
dioptric settings
D
Inspecting parfocality
4.
Engage
the
highest
magnification
position.
5.
Optimize
the
focus
using
the
focusing
drive.
6.
Engage
the
lowest magnification
positi
on.
7.
Do
not
look
into
the
eyepieces.
~Rotate
the
eyepieces
counterclockwise
in
the"+" direction
as
far
as
the
stop
(+5
diopters).
8.
Look
into
the
eyepieces
~
Slowly
rotate
each
eyepiece
individually
in
the"
-"
direction until
each
eye
sees
the object sharply
imaged.
9.
Engage
the
highest
magnification
position.
10.
Refocus
if necessary.
11.
Slowly
move the
magnification
changer
from
the
lowest magnification
to
the
highest.
The
image
sharpness
must
remain constant (parfocal). Otherwise, repeat
the
procedure.
Leica M St
ereomicroscopes-
Operation
21
Assentbly
22
The
components
1 a
Inc
ident-light
base with
ste~ge
pla
te
1 b
Transm
itted-light
base
with g
lass
stage
ple~te
2a
Side-faced
column
with dri
ve housing
, coarse/fine
2b
Side-faced
column
with dri
ve
housing, 300
mm
or
500
mm
3 Microscope carrier
4
Op
tics carr
ier
5
Interch
angeable
objective
6
Binocula
r tube
7 8
Wide-field eyepi
ece
for spectacle wearers, with
eyecups
Various
ErgoModule™
or accessories for
video,
photography,
drawi
ng,
fluorescence, coaxial incident li
ght
etc.
Le
1ca
M Stereomicroscopes- Assembly
Side-faced
column-+
incident-1
ight
base
Side-faced
column
-+
transmitted-light
base
Stand Incident-light
stan
d
~
Remove
the stage
plate.
~
Insert 3 Ali
en
screws
into
the und
erside
of the
baseplate
and
secure th
em
to
the
bottom
end
of
the
side
-faced column.
~Insert
the
stage plate.
To fit
the
microscope carrier and
the
ste
reomicroscope,
see
page
34
Transmitted-light
stand
~
Pu
ll
the
power
cable out
of
the
socket
in
the
stand.
~
Remove
the glass
stage
plate.
~
Carefully invert
the
transmitte
d-
light
base
and
remove 7 Alien
screws from t
he
base.
~
Lift
off
the
lower
par
t of the
base
.
~
Reverse
the upper
part of
the
base
.
~
Insert 3
Alien scr
ews
into
the
cover
pl
ate
from
beneath
and
secu
re
th
em
to
the
bottom
end
of t
he sid
e-
faced
column
.
~Replace
and
secure
the
base.
~
Insert
the
glass
stage
plate.
~Fit
t
he
microscope carrier
and
the
stereomicroscope
as
descri
bed
on
page 34.
Leica
M Stereomicroscopes- Assembly
23
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