Leica EM AFS2, EM FSP User Manual
Leica EM AFS2
Automatic Freeze Substitution System
Leica EM FSP
Freeze Substitution Processor
Leica EM AFS2
Freeze Substitution has never been easier…
Freeze Substitution (FS) of specimens in methanol, acetone or any other FS media at low temperatures is THE follow-on procedure to high pressure freezing and other cryo fixation methods.
Progressive Lowering of Temperature (PLT) allows substitution and resin infiltration of
chemically fixed specimens.
Finally, the sample is polymerized under UV light in the EM AFS2 and can be cut and immuno
labelled.
The Leica EM AFS2 provides all the features for …
Perfect results:
Reproducible
•
Excellent specimen visibility with
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– Internal LED illumination
– Stereomicroscope (optional)
-140 °C to +70°C working range
•
“Deep Freeze” allows sample transfer at temperatures below -140 °C
•
Transfer function “TF” excludes humidity and oxygen
FS chamber with new cryotube insert
•
Fast UV polymerization with LED UV lamp
•
LED UV lamp
Flexibility
Any choice of substitution system, e.g.
•
– Substitution Capsule and Flat Embedding System
– New Microtube Embedding System EM AFS2
– Sapphire Disk FS System
Trolley for mobility
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Ease of use
Intuitive programming
•
– 10 users
– 99 programs each
– 99 program steps each, 0 to 99 hrs 59 mins
– graphic display of substitution protocol
– menu prompts
Mouse controlled color screen
•
Memory stick to transfer programs
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Log file download on memory stick
•
Safety and convenience
Operator safety due to fume exhaust system
•
35 l Dewar
•
LN2filling from outside specimen chamber
•
LN2consumption enough for 5 day protocols
•
Stainless steel working platform
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