Leica EM CPD030 User Manual

Leica EM CPD030

Critical Point Dryer

Controlled Specimen Drying

Critical Point Drying

Critical point drying is an effi cient method for drying delicate
samples. It preserves the surface structure of a specimen which
could otherwise be damaged due to surface tension when chang­ing from the liquid to gaseous state.

The Leica EM CPD030 gently dries specimens of a biological
nature such as pollen, tissue, plants, insects, etc. as well as in­dustrial samples for MEMS (Micro Electro Mechanical Systems)
applications.

Before drying, many biological samples are commonly prepared
through fi xation and dehydration. The Leica EM TP is an ideal in­strument for such methods. It provides safe and automatic pro­cessing for follow-on critical point drying.

After drying, the SEM specimens are coated with a metal such
as gold, platinum or palladium to make their surfaces electri­cally conductive. Leica offers a range of high quality coating sys­tems such as the EM SCD005, EM SCD050, EM SCD500 and the
EM MED020 best suited for these processes.

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Key Features

• Vapor Compression Refrigeration System
for low C02 consumption

• Top loading system with safety screw-on cover,
ideal for fast and easy specimen insertion

• Controlled specimen drying for best results

• Approved specimen pressure chamber guarantees
absolutely safe operation

• Excellent visibility of the immersed specimen during
the CP process both from above and from the front

• Adjustable heating parameters for gentle specimen treatment

• Integrated automatic cooling/heating system

• Gas fl ow measuring device for precise release of
gaseous C02 after critical point drying

• Magnetic stirrer for better and faster mixing
of transitional fl uids

• Conveniently arranged control and display elements

• Space saving design with integrated control and supply units

• Wide selection of accessories

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Accessories

➀ Specimen transfer container

For transferring specimens immersed in transitional fl uid, such as
acetone or ethanol, to the pressure chamber of the critical point
dryer. The container has a bottom opening mechanism for the C02
exchange process.

➁ Specimen baskets (10 mesh/cm

Five baskets with cover designed to fi t in the specimen transfer
container for the subsequent critical point drying process.

➂ Specimen baskets (20 mesh/cm

Wire mesh specimen basket with a screw-on cover designed to fi t
the cover slip holding basket for sample transfer.

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➃ Universal specimen container

Specimen container with nine numbered chambers ideal for
routine work with a large number of different samples. For small
specimens, wire netting can be replaced with fi lter gauze or fi lter
paper.

➀➁

➂➃

➄ Cover slip container

Specimen container for cover slips and 20 mesh baskets for trans­ferring and processing specimens immersed in transitional fl uid to
the pressure chamber of the critical point dryer. The container has a
top opening mechanism for the C02 exchange process.

➅ Holder for cover slips

Cover slip holder with inserts which can be easily integrated into
the cover slip holding basket for processing.

➆ Holder for grids

Six slotted specimen holder for grids. Ideal for drying specimens
applied to fi lm-coated grids.

➇ Holder for fi lter discs

Specimen holder for six fi lter discs. Ideal for small specimens
such as bacteria, viruses, microorganisms, etc.

➄➅

➆➇

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Gentle Specimen Drying

of biological specimens….

Biopsy of dog gastric mucosa with Heliobacter salomonis.
Courtesy of Prof. M. H. Stoffel, University of Bern

… and samples for industrial processes.

Biopsy of dog gastric mucosa with Heliobacter bizzozeronii.
Courtesy of Prof. M. H. Stoffel, University of Bern

Microwave switch Lamella of SI-wafer

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The Leica Microsystems Life Science Division supports the
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Leica EM CPD030 - E - 3/09 Order No. 167060002

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