Leica EM AFS2
Automatic Freeze Substitution System
Leica EM FSP
Freeze Substitution Processor
Leica EM AFS2
Freeze Substitution has never been easier…
Freeze Substitution (FS) of specimens in methanol, acetone or any other FS media at low temperatures is THE follow-on procedure to high pressure freezing and other cryo fixation methods.
Progressive Lowering of Temperature (PLT) allows substitution and resin infiltration of
chemically fixed specimens.
Finally, the sample is polymerized under UV light in the EM AFS2 and can be cut and immuno
labelled.
The Leica EM AFS2 provides all the features for …
Perfect results:
Reproducible
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Excellent specimen visibility with
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– Internal LED illumination
– Stereomicroscope (optional)
-140 °C to +70°C working range
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“Deep Freeze” allows sample transfer at temperatures below -140 °C
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Transfer function “TF” excludes humidity and oxygen
FS chamber with new cryotube insert
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Fast UV polymerization with LED UV lamp
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LED UV lamp
Flexibility
Any choice of substitution system, e.g.
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– Substitution Capsule and Flat Embedding System
– New Microtube Embedding System EM AFS2
– Sapphire Disk FS System
Trolley for mobility
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Ease of use
Intuitive programming
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– 10 users
– 99 programs each
– 99 program steps each, 0 to 99 hrs 59 mins
– graphic display of substitution protocol
– menu prompts
Mouse controlled color screen
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Memory stick to transfer programs
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Log file download on memory stick
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Safety and convenience
Operator safety due to fume exhaust system
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35 l Dewar
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LN2filling from outside specimen chamber
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LN2consumption enough for 5 day protocols
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Stainless steel working platform
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One System – Many Solutions
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Leica EM FSP
The Leica EM FSP (“Freeze Substitution Processor”) is an automatic
reagent handling system. Mounted on the Leica EM AFS2, it dispenses
reagents for both FS and PLT. It automatically dilutes FS media and resins from 100%
reagent containers. An integrated LED UV lamp allows immediate polymerization of the samples.
Automatic process
One step preparation
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Reduced workload
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Increases reproducibility
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Perfect results
Efficient UV polymerization with
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built-in LED UV lamp
Flexibility
FS and PLT
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Choice between Flat Embedding or Flow
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Through Ring System
FS chamber with FSP accessories
Ease of use
Graphic display of substitution protocol
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“Check” function guides operator for filling reagents
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Log file download on memory stick
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Safety and convenience
Minimizes operator contact with toxic media
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Reduces risk of specimen loss
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Leica EM AFS2 and EM FSP
1 2 3
4 5 6
Image 1–3: Mouse, cartilage
Image 4–6: Yeast (S. cerevisiae)
Image 7–8: Liver HEPG2 cells
Courtesy of E.G. van Donselaar,
B.M. Humbel, Utrecht University,
The Netherlands;
J.W. Slot, University Medical Center
Utrecht, The Netherlands
Image 9–11: Rat, kidney
7 8
Courtesy of L. Edelmann,
University Homburg, Germany
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5
10 11
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Leica Mikrosysteme GmbH
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Leica EM AFS2_EM FSP - E - 6/05 Order No 204002, Printed on chlorine-free bleached paper. Printed in Germany.
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