Leica EG1160 User Manual

Leica EG 1160
Paraffin Embedding Center
Instruction manual
Leica EG1160 V4.1 English – 05/2001 Always keep this manual near the instrument!
Read carefully prior to operating the instrument!

1. Important information

Serial No. .................................................
Year of manufacture .................................................
Manufactured in: Federal Republic of Germany
The information, numerical data, notes and value judg­ments contained in this manual represent the current state of scientific knowledge and state-of-the-art tech­nology as we understand it following thorough investi­gation in this field. We are under no obligation to up­date the present manual periodically and on an ongo­ing basis according to the latest technical develop­ments, nor to provide our customers with additional copies, updates etc. of this manual.
For erroneous statements, drawings, technical illus­trations etc. contained in this manual we exclude li­ability as far as permissible according to the national legal system applicable in each individual case. In par­ticular, no liability whatsoever is accepted for any fi­nancial loss or consequential damage caused by or related to compliance with statements or other infor­mation in this manual.
Statements, drawings, illustrations and other informa­tion as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our products. These are determined only by the contract provisions agreed between our­selves and our customers.
Leica Microsystems Nussloch GmbH
Heidelberger Strasse 17-19 D-69226 Nussloch Germany
Leica reserves the right to change technical specifi­cations as well as manufacturing processes without prior notice. Only in this way is it possible to continu­ously improve the technology and manufacturing tech­niques used in our products.
This document is protected under copyright laws. Any copyrights of this document are retained by Leica Microsystems Nussloch GmbH.
Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy, microfiche, web cam or other methods – including any electronic systems and media – requires express prior permis­sion in writing by Leica Microsystems Nussloch GmbH.
For the instrument serial number and year of manu­facture, please refer to the name plate at the back of the instrument.
Telephone 0 62 24/143 - 0 Telefax 0 62 24/143 - 200 eMail: histo_info@leica-microsystems.com Internet: http://www.histo-solutions.com
Leica EG 1160 – Paraffin Embedding Center 3
© Leica Microsystems Nussloch GmbH

2. Table of contents

1. Important information..................................................................................................................................... 3
2. Table of contents............................................................................................................................................. 4
3. Installation........................................................................................................................................................ 5
4. Technical data ................................................................................................................................................. 6
5. General description ........................................................................................................................................ 7
6. Safety precautions .......................................................................................................................................... 8
7. Leica EG 1160 – Overview............................................................................................................................ 10
8. Components and features ............................................................................................................................ 12
9. Controls / Display .......................................................................................................................................... 16
10. Operation ........................................................................................................................................................ 17
11. Tissue embedding ......................................................................................................................................... 24
12. Cleaning, maintenance, service................................................................................................................. 26
13. Troubleshooting ............................................................................................................................................ 28
14. Ordering information .................................................................................................................................... 31
15. Warrenty and service ................................................................................................................................... 32
4
Instruction manual V 4.1 – 05/2001
The Leica EG 1160 is a compact bench-top unit and can be set up easily. All components come in a cardboard box. The components that are packed separately can be attached to the instrument easily (see page 5 "Part list").
1. Transport and assembly position
The Leica EG 1160 must never - not even for a short while - be positioned as shown. These positions will inevitably cause damage to the condenser. Therefore, please check the Tip-N-Tell indicators which are attached to the package upon receipt.
Should it become necessary to bring the instrument in a position other than normal, lay the instrument on its back turning it slowly and carefully.

3. Installation

2. Unpacking
The instrument may o n l y be lifted holding it at the sides of the base plate of the housing. Dot not carry the instrument holding it at the top cover, at the front handholds of the removable trays or the dispenser. Risk of fracture!
3. Place of installation
The instrument should be set up on a stable, plane laboratory table. Please
ensure that there is no air condition vent nearby. The location must fulfil the following conditions to ensure that air circulation is not affected. The back of the instrument must be at least 15 cm away from the wall. The shipment includes two spacers which must be attached to the back. Non-compliance with this minimum distance can cause substantial damage to the instrument and will invalidate the warranty.
4. Power supply
Prior to connecting the instrument to the mains outlet, please ensure that the power supply specified on the nameplate complies with the local mains conditions.
5. Foot pedal
The foot pedal is connected to the socket in the right-hand bottom corner on the back.
Leica EG1160 – Paraffin Embedding Center 5

4. Technical data

Power supply: 115 V / 60 Hz
230 V / 50 Hz
Fuses: 115V/60Hz F1, F2 = T5.0 A 230V/50Hz acc. to IEC 127-2
Power consumption: 800 VA
Operating temperature range: 18°C - 35°C
Cooling system: Refrigerant: 140 g ±5g R134A Compressor oil: 150 ccm
EMKARATE RL15S
Temperature control
Dimensions: (LxDxH) 910 x 575 x 320 mm
Weight: 50 kg
Capacities:
Paraffin reservoir: 3 l Cassette bath: approx.100 cass. Heated recessed area: 8 cassettes Cold plate: approx. 60 blocks
Programmable parameters:
Temperature
Paraffin reservoir Mold warmer Cassette bath Work surface
Cold plate Workdays Daily work times (beginning, end) Weekday Time
Operation either manual or via the foot pedal.
Functional unit Temperature Heated Separate Warm-up time
range separately temperature prior to operation
control
Paraffin reservoir 45 - 70°C, + + 4 h
± 1°C increments
Mold warmer 35 - 70°C + + 1 h
± 1°C increments
Cassette bath 45 - 70°C + + 1.5 h
± 1°C increments
Work area 45 - 70°C + + 1 h
± 1°C increments
Cold plate - 5°C - - 1 h
Forceps holder right 70°C + -
Forceps holder left 70°C + ­(not basic instrument)
Paraffin dispenser 45 - 70°C + ­+ pump
* at an ambient temperature of 22°C and at an air humidity of 60%
All heated components include overheat protection.
6 Instruction manual V4.0 – 4/98

5. General description

Features
1. Paraffin flow rate is pump con trolled.
2. Paraffin flow rate adjustable in 10 increments
3. Paraffin quantity level indicated in the display
4. Separate paraffin collecting tray
5. Removable cassette bath
6. Forceps holder on the right (ba sic instrument) or on the right and left (not basic instrument)
7. Paraffin dispenser with inte grated illumination
8. User menu available in 5 lan guages
9. Fault detection by error codes in the display
10. Optional accessories: Magni fier, vacuum attachment, fiber optical light guide (for connec tion to the coldlight souces of the Leica CLS series)
The Leica EG 1160 is a modern tis­sue embedding center providing all features required for rapid, conve­nient and efficient paraffin embed­ding of tissue including a separately heated 3-liter capacity paraffin res­ervoir. Paraffin quantity level shown on LCD display in two steps. Sepa­rately heated paraffin delivery sys­tem with integrated filter and pump ensure a smooth, consistent paraf­fin flow. The paraffin flow rate is ad­justable in 10 increments.
The paraffin outlet nozzle can be op­erated either manually or via foot pedal. A non-glare illumination is in­tegrated at the paraffin dispenser outlet to provide a well lighted work area.
The separately heated mold warmer to the right of the paraffin reservoir is designed to store and heat the molds prior to embedding.
The work area is divided into three parts: Cassette storage, working and embedding area and cold plate. The cassettes containing the tissue are stored in a separately heated, re­movable tray with a capacity of approx. 100 specimen capsules.
The large work area ensures conve­nient block removal and easy access to the cassettes. The forceps hol­der(s) is/are designed to accommo­date three forceps and is/are inte­grated in the instrument. The heated recessed area behind the dispenser handle is designed to keep the par-
affin in the cassette molten. Excess paraffin can be drained through an outlet after the removing the stopper.
In front of the paraffin dispenser out­let a refrigeration spot is integrated in the cold plate ensuring optimal cooling. The large cold plate pro­vides space for more than 60 embed­ding molds. A removable tray is lo­cated under the work area to collect excess paraffin drained from the work area.
The Leica EG 1160 is operated and programmed via a menu-driven con­trol board. The menu is displayed in German, English, French, Spanish or Italian as selected. All operations are microprocessor-controlled.
Malfunctions are indicated by a nu­merical error code in the display. A battery backup prevents loss of all programmed parameters if a power failure occurs.
The individual functional units of the Leica EG 1160 are ergonomically po­sitioned to enable rapid and conve­nient operation. The instrument may be operated by qualified personnel only in accordance with this operat­ing instruction manual.
If used in accordance with the op­erating instructions and appropriate maintenance is ensured, the tissue embedding center will operate trouble-free and give perfect embed­ding results for many years.
7Leica EG1160 – Paraffin Embedding Center

6. Safety precautions

- The instrument may be used by qualified personnel only.
- To avoid any operating mistakes from the beginning, it is necessary to read the operating instructions to familiarize oneself with the technical details.
- Paraffin is flammable and should be handled with care. Spillage should be avoided. Paraffin on the surfaces must not be removed with sharp tools as it would ruin the coating. It should be avoided to allow xylene to react on all surfaces.
Caution:
Xylene is a flammable organic solvent. Its flash point is between 27 and 32°C. Xylene vapours are heavier than air and can easily catch fire on hot surfaces or sparks even over a greater distance.
- Illumination: Before replacing the lamp, the tissue embedding center must be turned off with the main switch.
- To clean the condenser fins, switch the instrument off with the main switch and disconnect it from mains.
- To replace the fuses, the power cord must be disconnected from the power supply. Only fuses that are accessible from outside can be re­placed. If you need further information, please contact your local Leica sales office or dealer.
- When operating, the paraffin reservoir, mold warmer, cassette bath, work area and forceps holder are all hot.
Caution: Risk of burning! Note: Combustible and flammable substances must not be stored near
the instrument!
8
Instruction manual V 4.1 – 05/2001
- When using the optional vacuum attachment, please ensure that, af-
ter infiltration, the vacuum is neutralised very slowly. Therefore, open the aeration knob very cautiously. Air entering too rapidly could cause hot paraffin to splash and should therefore be avoided.
- Prior to connecting the instrument to the power supply in the labora-
tory, please make sure that the power supply complies with the val­ues specified on the nameplate. The tissue embedding center should be connected to a grounded socket only.
6. Safety precautions
- The equipment must not be set up near an air condition vent or should
not be exposed to direct sunlight!
- Full refrigeration capacity is ensured only if the clearance on the back
of the instrument is not less than 15 cm. Therefore, it is necessary to mount the spacers provided.
- Prior to any maintenance and service action, the tissue embedding
center must be disconnected from the power supply.
Leica EG1160 – Paraffin Embedding Center 9
7. Leica EG 1160 – Overview
Illumination button (3) LCD display (2)
Leica EG1160
Menu button (4) Arrow UP/DOWN
buttons (6)
SET/ACT button (5)
Forceps holder (11a)* (only on model with additional forceps holder on the left)
Cover cap for illumination (23)
ON/OFF button (7)
Contol panel (1)
Dispenser with integrated illumination (8)
Paraffin reservoir (13)
Mold warmer (14)
Cold plate (17) Dispenser handle
extension clip (29)
Dispenser handle (9)
10
Refrigeration spot (16)
Paraffin collecting tray (18)
Work area (20)
Cassette bath (15)
Heated area for cassettes (10)
Forceps holder (11)
Instruction manual V 4.1 – 05/2001
+ 22 hidden pages