Leica EG 1150 H V2.2 User Manual

Leica EG1150H

Paraffin Embedding

Station

Instruction Manual

Leica EG1150H

V2.2 English, Rev. B - 12/2009

Always keep this manual near the instrument.

Read carefully prior to operating the instrument.

IMPORTANT NOTE

The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and state-of-the-art technology as we understand it following thorough investigation in this field.

We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.

For erroneous statements, drawings, technical illustrations, etc. contained in this manual we exclude liability as far as permissible according to the national legal system applicable in each individual case. In particular, no liability whatsoever is accepted for any financial loss or consequential damage caused by or related to compliance with statements or other information in this manual.

Statements, drawings, illustrations and other information as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our products.

Published by:

Leica Biosystems Nussloch GmbH Heidelberger Str. 17 - 19

D-69226 Nussloch Germany

Phone: +49 (0)6224 143-0

Fax: +49 (0)6224 143-268

Internet: http://www.leica-microsystems.com

These are determined only by the contract provisions agreed between ourselves and our customers.

Leica reserves the right to change technical specifications as well as manufacturing processes without prior notice. Only in this way is it possible to continuously improve the technology and manufacturing techniques used in our products.

This document is protected under copyright laws. Any copyrights of this document are retained by Leica Biosystems Nussloch GmbH.

Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy, microfiche, web cam or other methods — including any electronic systems and media — requires express prior permission in writing by Leica Biosystems Nussloch GmbH.

For the instrument serial number and year of manufacture, please refer to the name plate at the left side of the instrument.

Leica EG1150H – Paraffin Embedding Station

Table of contents

Important notes ..........................................................................................................................................................

1.1Symbols used in the text and their

	meanings .....................................................................................................................................................................	5
1.2	Specified use and application .................................................................................................................................	5
1.3	User group ...................................................................................................................................................................	5
1.4	Instrument type ...........................................................................................................................................................	5
2.	Safety ..........................................................................................................................................................................	6
2.1	Safety regulations ......................................................................................................................................................	6
2.2	Safety instructions .....................................................................................................................................................	6
2.3	Built-in safety devices ...............................................................................................................................................	8
3.	Instrument components and specifications .........................................................................................................	9
3.1	Specifications .............................................................................................................................................................	9
3.2	Overview – instrument parts ..................................................................................................................................	10
3.3	Instrument specifications .......................................................................................................................................	11
4.	Commissioning .........................................................................................................................................................	12
4.1	Unpacking and installation .....................................................................................................................................	12
4.3	Package contents ....................................................................................................................................................	13
4.2	Location conditions..................................................................................................................................................	13
4.4	Necessary assembly work .....................................................................................................................................	14
4.5	Optional accessories ...............................................................................................................................................	16
4.6	Electrical connection ..............................................................................................................................................	17
5.	Operation ...................................................................................................................................................................	18
5.1	Instrument parts/functions .....................................................................................................................................	18
5.2	Switching the instrument on ..................................................................................................................................	23
5.3	Control panel functions ...........................................................................................................................................	24
5.4	Operating modes ......................................................................................................................................................	25
5.5	Time-program control .............................................................................................................................................	26
5.6	Instrument heater.....................................................................................................................................................	28
6.	Cleaning and maintenance ....................................................................................................................................	30
6.1	Cleaning the instrument ..........................................................................................................................................	30
6.2	Maintenance instructions ......................................................................................................................................	31
7.	Troubleshooting .......................................................................................................................................................	32
7.1	Possible faults ...........................................................................................................................................................	32
7.2	Replacing the halogen lamp ...................................................................................................................................	35
7.3	Changing a fuse ........................................................................................................................................................	36
8.	Warranty and service .............................................................................................................................................	38

4	Instruction Manual V 2.2, Rev. B – 12/2009

1.Important notes

1.1Symbols used in the text and their meanings

Warnings and cautions

appear in a gray box and are marked by a warning triangle .

Notes,

i.e. important information for the user, appear in a gray box and are marked with the symbol .

Solvents and reagents that are inflammable are marked with this symbol.

This warning symbol indicates the surfaces on the instrument that are hot during operation.

Avoid direct contact to prevent risk of burning.

Figures in brackets refer to item

(5)numbers in figures.

Function keys that have to be

pressed on the input screen, are dis- ENTER played in bold type and capital let-

ters.

1.2 Specified use and application

The Leica EG1150H is a modern paraffin embedding station with microprocessor control.

It is designed for embedding histological tissue specimens in molten paraffin for use in pathology laboratories and only for the following tasks:

•Melt solid paraffin for sample embedding and maintain the molten paraffin at the required temperature.

•Fill the molds, in which the tissue specimens were placed, with paraffin.

•Heat and maintain the temperatures of embedding cassettes with specimens and molds as well as the required forceps.

Any other use of the instrument will be considered as improper use!

1.3 User group

•The Leica EG1150H may only be operated by qualified personnel.

•The user must read the operating instructions supplied and be familiar with all the instrument’s technical details before any work on the instrument can be carried out.

1.4 Instrument type

All information in this instruction manual applies only to the instrument type indicated on the title page.

A name plate with the serial number is attached to the back of the instrument.

Leica EG1150H – Paraffin Embedding Station

2.Safety

Be sure to comply with the safety instructions and warnings provided in this chapter.

Be sure to read these instructions, even if you are already familiar with the operation and use of other Leica products.

2.1Safety notes

This instruction manual contains important instructions and information regarding the operational safety and maintenance of the instrument.

The instruction manual is an important part of the product, which must be read carefully prior to start-up and use and must always be kept near the instrument.

If additional requirements on accident prevention and environmental protection exist in the country of operation, this instruction manual must be supplemented by appropriate instructions to ensure compliance with such requirements.

This instrument has been built and tested in accordance with the safety regulations for electrical measuring, control, regulating and laboratory devices.

To maintain this condition and ensure safe operation, the user must observe all notes and warnings contained in this Operating Manual.

For current information about applicable standards, please refer to the CE declaration for the instrument and to our Internet site: http://www.leica-microsystems.com

The protective devices on both instrument and accessories may neither be removed nor modified. Only service personnel qualified by Leica may repair the instrument and access the instrument’s internal components.

2.2Warnings

The safety devices installed in this instrument by the manufacturer only constitute the basis for accident prevention. Primarily responsible for accident-free operation is above all the owner of the instrument and, in addition, the designated personnel who operates, services or cleans the instrument.

To ensure trouble-free operation of the instrument, make sure to comply with the following instructions and warnings.

6	Instruction Manual V 2.2, Rev B – 12/2009

2. Safety

Safety instructions – safety regulations on the instrument itself

Safety regulations marked with a warning triangle on the instrument itself mean that when operating or exchanging respective parts of the instrument, the correct operating steps as described in the instruction manual supplied, must be adhered to. Non-obser- vance can cause accidents, injuries and/or damage to the instrument/accessories.

Certain surfaces of the instrument are hot during operation under normal conditions. They are marked with this warning sign. Touching these surfaces can cause burns.

Safety instructions – transport and installation

After unpacking the instrument it may only be transported in an upright position. Place the instrument on a laboratory table and adjust it to a horizontal position. The instrument must not be exposed to direct sunlight (window)!

Plug the instrument only into a grounded mains socket. The protective effect may not be eliminated by an extension cable without a protective grounding conductor.

The instrument automatically recognizes the applied voltage/frequency.

The installation location must be well-ventilated; there should be no ignition sources there of any kind.

The instrument may not be operated in hazardous locations.

Extreme temperature fluctuations between storage facility and setup site as well as high humidity may cause condensation to form. In this case, wait at least two hours before switching on.

Safety instructions – working with the instrument

Paraffin is flammable and should therefore be handled with due care. Do not use sharp tools to remove solidified paraffin from the work areas, as this may destroy the coating on the surface. Use the plastic spatula supplied with the instrument.

During operation, the paraffin reservoir, mold warmer, cassette warmer, work area as well as the forceps holder are hot.

Risk of burning!

Do not store any combustible and flammable substances near the instrument. There is a fire hazard if work with an exposed flame (e.g. Bunsen burner) is carried out in the direct vicinity of the instrument (solvent vapors). Therefore a minimum safety distance of 2 meters must be adhered to!

Leica EG1150 H – Paraffin Embedding Station

2.Safety

Hazards – servicing and cleaning

Switch off the instrument each time before servicing and pull out the mains plug.

When using cleaners, please comply with the safety instructions of the manufacturer and the laboratory safety regulations.

Before changing defective fuses, the instrument has to be disconnected from the mains. Only fuses that are easily accessible may be replaced by the user.

Set the standby switch to “Standby“ () when replacing the halogen lamp and pull the power cable out of the mains socket.

During operation and cleaning, do not allow any liquid to penetrate inside the instrument and the transporting arm.

2.3 Built-in safety devices

The instrument is equipped with the following safety features and devices:

Fuses in the heating elements

All resistance mats of the instrument are equipped with overheating fuses, which switch the heating element off if overheated.

Automatic circuit breaker in standby switch

An automatic circuit breaker is located in the standby switch. This circuit breaker separates the power electronics from the mains power supply in the event of a short-circuit.

In this case, the standby switch jumps to the position “0“ = Off.

Note that the only way the user has for complete disconnection from the mains supply is disconnection of the mains plug.

8	Instruction Manual V 2.2, Rev. B – 12/2009

	3. Instrument components and specifications

3.1 Specifications
General data
Approvals:	The approval symbols relating to this instrument are located
	on the rear of the instrument next to the nameplate.
Power voltages:	100-120 V, 50/ 60 Hz
	230-240 V, 50/ 60 Hz
Input:	1100 VA
Class of protection1:	I
Degree of pollution1:	2
Overvoltage category:	II
Operating temperature range:	+18 °C to + 40°C
Working temperatures:	55 °C to 70 °C, adjustable in 5 degree (K) increments.
Relative air humidity:	maximum 60 %, non-condensing.

1) according to IEC-1010, UL 3101, EN 61010

Fuses

Standby switch:

Fine-wire fuses 6.3 x 32 mm:

Fuse type:

Dimensions and weight

Dimensions:

Height:

Width:

Depth:

Weight:

Capacities

Paraffin reservoir:

Cassette warmer:

Mold warmer:

Programmable parameters

Circuit breaker manufactured by ETA, model 3120-F421-P7T1-W01D-5A

2x T 2.0 A; 2x T 3.2 A; T2x 4.0 A; T2x 5.0 A; T2x 6.25 A Co. Schurter: type Fst

360 mm

500 mm

640 mm approx. 22 kg

approx. 3 l

approx. 100 cassettes approx. 50 molds

Temperature:	Paraffin reservoir/dispenser
	(heating-up time = 4h)
	Mold warmer, cassette warmer, work area
Time:	Working days, current weekday
	Working times (start, end), time

Leica EG1150 H – Paraffin Embedding Station

3.Instrument components and specifications

3.2 Overview – instrument parts

Standby	Paraffin reservoir
switch	with lid	Control panel	Work area illumination

Forceps holder

Mold warmer lid

Mold warmer

Paraffin collection tray left

Dispenser

Cassette warmer lid

Cassette warmer

Work area

Paraffin collection tray right

Refrigeration spot

Fig. 1

10	Instruction Manual V 2.2, Rev. B – 12/2009

3. Instrument components and specifications

Instrument rear view

Secondary fuses F1-F3

Secondary fuses F4-F5

Heat sink

Port for power cord

Port for foot switch

Sliding elements

Fig. 2

3.3Instrument specifications

•Paraffin reservoir with a capacity of 3 liters.

•The paraffin flow is activated by means of a height-adjustable, pivotable clip – activated either manually by the mold or a foot switch (optional).

•Controllable flow rate.

•Removable, heatable (indirectly, via the work surface) paraffin collection trays.

•Spacious, easy-to-clean, heated work area, with integrated refrigeration spot, also for extra large cassettes (‘Super Mega Cassettes’) with paraffin flow system.

•Warmers for cassettes and/or molds with sliding lid, removable and interchangeable.

•Removable, heated forceps holder for 6 forceps, accessible from both sides.

•Optimum illumination of the work surface by individually adjustable halogen lamp.

•Temperature range of cassette and mold warmer, work area and paraffin reservoir adjustable from 55°C to 70°C.

•Permanent temperature display for all the work areas.

•The beginning and end of the work time and work days can be programmed.

Leica EG1150 H – Paraffin Embedding Station

4.Commissioning

4.1 Unpacking and installation

The unpacking instructions are attached to the outside of the transport crate. Fig. 3 shows the design of the original packaging.

The numbers indicate the sequence of disassembling and reassembling.

• To lift up the instrument (6), hold it at the front and rear of the base plate (Fig. 4) and lift it from the foam padding (8).

Important!

Lifting the instrument by the dispenser (10) or paraffin reservoir (11) can cause serious damage.

• After unpacking the instrument it should only be transported in a horizontal position.

• Two slide faces (9) at the rear of the base plate help to reposition the instrument on the table. To reposition, slightly lift the instrument at the front of the base plate and slide it on the slide faces.

• The instrument should be set up in such a way that the air circulation is not affected. A smooth operation is ensured only when the instrument is set at a minimum distance of 15 cm from walls and furniture.

Fig. 3

Fig. 4

12	Instruction Manual V 2.2, Rev B – 12/2009

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