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Instruction Manual
Leica EG1150C
V2.1 Rev B, English – 01/2010
Always keep this manual near the instrument.
Read carefully prior to operating the instrument.
Leica EG1150 C
Cold Plate
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IMPORTANT NOTE
The information, numerical data, notes and value
judgments contained in this manual represent the
current state of scientific knowledge and state-ofthe-art technology as we understand it following
thorough investigation in this field.
We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with
additional copies, updates etc. of this manual.
For erroneous statements, drawings, technical
illustrations etc. contained in this manual we
exclude liability as far as permissible according
to the national legal system applicable in each
individual case. In particular, no liability whatsoever is accepted for any financial loss or consequential damage caused by or related to
compliance with statements or other information in this manual.
Statements, drawings, illustrations and other information as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our
products.
These are determined only by the contract provisions agreed between ourselves and our customers.
Leica reserves the right to change technical
specifications as well as manufacturing processes without prior notice. Only in this way is it
possible to continuously improve the technology and manufacturing techniques used in our
products.
This document is protected under copyright
laws. Any copyrights of this document are retained by Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of
any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – including any electronic systems and media – requires express prior permission in writing by
Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the name plate at
the back of the instrument.
© Leica Biosystems Nussloch GmbH
Issued by:
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Phone: +49 (0)6224 143-0
Fax: +49 (0)6224 143-268
Internet: http://www.leica-microsystems.com
Leica EG1150C
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Table of contents
1. Important notes ............................................................................................................................................. 5
1.1 Symbols used in the text and their meanings ..................................................................................5
1.2 User group ............................................................................................................................................. 5
1.3 Specified use and application ............................................................................................................ 5
1.4 Instrument type ..................................................................................................................................... 5
2. Safety .............................................................................................................................................................. 6
2.1 Safety regulations................................................................................................................................. 6
2.2 Safety instructions ............................................................................................................................... 6
2.3 Built-in safety devices ......................................................................................................................... 7
3. Instrument components and specifications ............................................................................................ 8
3.1 Overview – instrument parts............................................................................................................... 8
3.2 Technical data ....................................................................................................................................... 9
4. Commissioning ........................................................................................................................................... 10
4.1 Location conditions ............................................................................................................................ 10
4.2 Delivery range ..................................................................................................................................... 10
4.3 Unpacking and installing ................................................................................................................... 11
4.4 Power supply ....................................................................................................................................... 12
5. Operation ...................................................................................................................................................... 13
5.1 Switch on the instrument .................................................................................................................. 13
5.2 Replacing the secondary fuse .......................................................................................................... 13
5.3 Malfunctions ....................................................................................................................................... 14
6. Cleaning and maintenance ....................................................................................................................... 15
6.1 Cleaning the instrument..................................................................................................................... 15
6.2 Maintenance instructions ................................................................................................................. 15
7. Warranty and service ................................................................................................................................ 16
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Instruction Manual V 2.2 – 01/2010
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1. Important notes
1.1 Symbols used in the text and their
meanings
Warnings and cautions
appear in a gray box and are marked
by a warning triangle .
Notes,
i.e. important information for the user,
appear in a gray box and are marked
with the symbol .
(5)
Figures in brackets refer to item numbers in figures.
1.2 User group
• The Leica EG1150C may only be operated by
qualified personnel.
1.3 Specified use and application
The Leica EG1150C is a cold plate for chilling
and blocking out histological tissue samples in
paraffin blocks.
Any other use of the instrument will be
considered as improper use!
1.4 Instrument type
All information in this instruction manual applies
only to the instrument type indicated on the title
page.
A name plate with the serial number is attached
to the back of the instrument.
• The user must read the operating instruc-
tions supplied and be familiar with all the instrument’s technical details before any work
on the instrument can be carried out.
Leica EG1150C
Fig. 1
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2. Safety
Please pay particular attention to the safety regulations and safety instructions in this section.
Please also read this information, even if you are familiar with handling and using a Leica instrument.
2.1 Safety regulations
This instruction manual contains important instructions and information regarding the operational safety and maintenance of the instrument.
The instruction manual is an important part of
the product. It must be read carefully before using the instrument for the first time and always
kept near the instrument.
It may be necessary to add appropriate instructions to this instruction
manual, if required by existing national regulations on accident prevention or environmental protection in the
country of the operating authority.
The protective devices on the instrument and its accessories must not be removed or modified.
The instrument may only be opened and repaired by authorized Leica servicing technicians.
2.2 Safety instructions
This instrument has been built and tested in accordance with the following safety regulations
on electrical measuring, control, regulating and
laboratory devices.
In order to maintain this condition and to ensure
safe operation, the operator must observe the
instructions and warnings contained in this instruction manual.
For current information about applicable standards, please refer to the CE declaration of conformity on our Internet site:
www.leica-microsystems.com
The safety devices provided on this instrument by the manufacturer only constitute the basis
for protection against accidents. The main responsibility for accident prevention while using
the instrument rests with the employer where the instrument is operated, and with the persons
he appoints for operating, servicing or cleaning the instrument.
To ensure a safe operation of the instrument at all times, the following instructions and warnings should be observed.
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Instruction Manual V 2.2 – 01/2010
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Safety instructions – safety regulations on the instrument itself
• This device may only be used by trained laboratory technicians. It may only be used according
to its intended use and the instructions in this instruction manual.
• Safety regulations marked with a warning triangle on the instrument itself mean that when
operating or exchanging respective parts of the instrument, the correct operating steps as
described in the instruction manual supplied, must be adhered to. Nonobservance can cause
accidents, injuries and/or damage to the instrument/accessories.
Safety instructions – transport, installation and operation
• After unpacking the instrument it may only be transported in an upright position.
• Before connecting the device to a power source, ensure that the voltage indicated on the type
plate matches the voltage available at the place of installation.
The unit must be connected only with the supplied power cable and only to a grounded power
receptacle. Do not use an extension cord.
• The minimum voltage (see specifications) must be maintained while starting the refrigeration
unit.
The compressor needs a starting current of approx. 25 A. For correct operation, a consistent
power supply conforming to the instrument’s specifications is required. Please ensure that
your electrical installation fulfills these preconditions prior to installing the unit.
Nonobservance causes damage to the instrument.
• Switch off the instrument each time before servicing, repairing or cleaning, and pull out the
power plug.
2. Safety
2.3 Built-in safety devices
Short-
circuit
Leica EG1150C
Automatic circuit breaker in the main switch
• An automatic circuit breaker with a lit switch
is located in the main switch. This circuit
breaker separates the unit from the mains
power supply in the event of a short-circuit.
• In this case, the main switch jumps to the
position “0“ = Off.
Fig. 2
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3. Instrument components and specifications
3.1 Overview – instrument parts
• The instrument is distinguished by a simple, modular design and a powerful refrigeration unit with precisely controlled cooling performance.
• High temperature consistency ensures homogeneous blocking out and
prevents tearing of the paraffin blocks.
• High cooling performance ensures that the instrument’s working temperature is reached quickly.
• Optimized temperature distribution in the cold plate prevents dripping
condensation.
• The generously-dimensioned cooling surface has room for around 70
blocks.
• Designed for use with the Leica EG1150 H paraffin embedding station.
Illuminated
on-off switch
(automatic fuse)
Refrigeration
system
(inside)
Hinged hood
(optional)
Cooling surface
Adjustable feet,
front and back
Fig. 3
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Instruction Manual V 2.2 – 01/2010
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3.2 Technical data
General specifications
Approvals: The approval symbols relating to this instrument are
3. Instrument components and specifications
located on the rear of the instrument next to the
nameplate.
Voltage of power supply:
The device requires a specific AC
voltage, see table at right. Instrument
type acc. to identification label.
AC ±10 %
Maximum input: 350 VA
Maximum starting current (5 s): 25 A
Class of protection
Mains power fuse 100/120 V: ETA circuit breaker type 3120-F421-P7T1
Mains power fuse 230/240 V: ETA circuit breaker type 3120-F421-P7T1
Miniature fuse 6.3 x 32 mm: T 1.0 A, Schurter: type Fst
Degree of pollution
Overvoltage category: II
Operating temperature range: +18 °C to +40 °C
Working temperature range: - 5 °C, up to a room temperature of approx. +28°C
Relative air humidity: maximum 80 %, non-condensing
1)
according to IEC-1010, UL 3101, EN 61010
Refrigeration unit
Refrigeration performance*: 110 W
Safety factor: 3
Refrigerant: 80 g ± 5 g R 134a
Compressor oil: 180 cm
Instrument type Voltage of power supply
EG1150 C-1 230 V / 50 Hz
EG1150 C-3 120 V / 60 Hz
EG1150 C-8 240 V / 50 Hz
EG1150 C-11 100 V / 50 - 60 Hz
1
:I
1
:2
3
Emkarate RL 15 s, ICI
W19DB4-10A
W19DB4-4A
*as per CECOMAF
Liquid temperature: 55 °C
Evaporation temperature: -25 °C
Dimensions and weight
Width: 360 mm
Depth: 650 mm
Height: 360 mm
Weight: 23 kg
Leica EG115 0 C
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4. Commissioning
4.1 Location conditions
4.2 Delivery range
• Stable, vibration-free laboratory table with horizontal, flat table top, as
far as possible vibration-free ground.
• No direct sunlight or strong temperature fluctuations. Room temperature throughout between +15 °C and +28°C.
At a room temperature of > +28°C the working temperature of the
cold plate of -5°C may not be reached at all points.
• Relative air humidity maximum 80%, non-condensing.
• The instrument should be set up in such a way that the air circulation
is not affected.
To ensure proper function, there must be gap of at least 15 cm behind the instrument. Failure to observe this distance may result in
serious damage to the refrigeration unit of the device. The instrument should not be operated in hazardous locations.
The basic equipment for the Leica EG115OC cold plate contains the following components:
Order No.
• 1 basic instrument, Leica EG 1150 C
• 1 power cable see
p. 12
only for Australia:
• 1 power cable EU 14 0411 13558
• 1 mains transformer, 230/240 V 14 0469 31998
• 1 replacement fuse T 1.0 A, 6,3 x32mm 14 6943 01001
• 1 operating manual 14 0388 80002
10
• optionally: protective hood (acrylic)
Please compare the delivered components against the packing list
and your order.
Should there be any discrepancy, please contact the Leica distributor handling your order.
Instruction Manual V 2.2 – 01/2010
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4.3 Unpacking and installing
4. Commissioning
The unpacking instructions are attached to the
outside of the transport crate. Fig. 4 shows the
design of the original packaging.
The numbers indicate the sequence of disassembling and reassembling.
1
3
2
2
Please check the transport packaging
for external damage.
If you detect obvious damage, please
report it to your shipping agent immediately.
To lift device, grasp at the front on the base
plate and at the rear at the base of the housing
(arrows in Fig. 5), lift out of the shaped pad (4)
and place on a sturdy laboratory table.
The Leica EG1150 C must be transported in an upright, horizontal position at all times. It must not be inverted under any circumstances, even
for short periods, or stored on one of
its sides.
A waiting period of 4 hours must be
observed between transporting the instrument and switching it on. The
compressor oil agitated while moving
the instrument must return fully to its
original position.
Leica EG1150C
4
5
Fig. 4
Fig. 5
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4. Commissioning
4.4 Power supply
7
The refrigeration unit of the Leica EG1150C requires a specific voltage
and frequency (see “Technical data“ chapter) and is therefore supplied
with an AC power cable suitable to the instrument.
Please observe the following notes to prevent damage to the unit:
Before connecting the device to a power source, ensure that the
voltage indicated on the type plate (rear) matches the actual voltage available at the place of installation.
If this is not the case, do not plug the instrument in!
The unit must be connected only with the supplied power cable
and only to a grounded power receptacle.
Do not use an extension cord!
• Connect the plug (7) of the power cord to the connecting port (6).
• Plug the power cord into the wall outlet.
To connect the instrument, use one of the power
cords listed below:
Location Order No.
Australia: 14 0411 32565
Europe: 14 0411 13558
Italy: 14 0411 13561
6
Switzerland: 14 0411 13560
United Kingdom: 14 0411 27822
USA, Canada, Japan: 14 0411 13559
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Fig. 6
Instruction Manual V 2.2 – 01/2010
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5.1 Switch on the instrument
After the installation as described in Chapter 4 is complete, the Leica
EG1150 C is ready for use. Switch the instrument on with the on-off switch at
the front left-hand side (“I“ = ON). A lamp lights up in the switch to indicate
that the unit is operational and the refrigeration unit will start working.
Depending on the room temperature, the working temperature of the cooling
surface (-5 °C) will be reached in around 25 minutes (see also Ch. 4.1, p. 10).
The cooling surface may not be loaded with molds until the cooling time has elapsed. Otherwise, the working temperature of -5 °C
may not be reached.
5.2 Replacing the secondary fuse
5. Operation
A miniature fuse to protect the electronic components is located on the rear of the instrument.
Before replacing the fuse, always
switch the instrument off and pull the
power plug from the wall socket.
Use only miniature fuses of the type
specified in the 'Technical data'
chapter.
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Leica EG1150C
To replace the fuse, please proceed as follows:
• Using a screwdriver, lightly press the fuse
holder (9) and turn counterclockwise about
10
1
/4 turn.
• Remove the fuse holder and take out the
fuse (10).
• Replace it with a new fuse of the same type.
• Replace the fuse holder (9).
• Using a screwdriver, gently press the fuse
holder and turn it clockwise about 1/4 turn.
9
• Reconnect the instrument to an AC power
outlet and switch it on.
Fig. 7
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5. Operation
5.3 Malfunctions
If you cannot solve your problem using the help in the following table, please contact your
Leica customer service organization or the Leica dealer from whom you purchased the instrument.
Possible causesMalfunction Corrective action
Instrument doesn’t work.
Cooling unsatisfactory,
formation of condensation.
Compressor switches off after a
brief period of operation and
switches back on shortly
thereafter.
Compressor does not start.
Compressor switches off after a
short period of operation.
Power switch not turned on
or
the cut-out function of the
power switch activated.
Room temperature too high or
exposure to direct sunlight.
Inadequate air supply to ventilation unit.
Refrigeration system leak.
AC power does not match
specifications or
compressor defective.
Compressor defective.
Compressor defective.
Check instrument is connected,
then turn main power switch on.
Only for Great Britain:
Check the fuse in the mains
plug.
Check the cooling again at a
lower room temperature.
Check distance of instrument to
wall; it must be at least 15 cm.
The instrument must be serviced or repaired by a service
technician.
Please contact customer
service.
Check the voltage of power
supply.
The instrument must be serviced or repaired by a service
technician.
Please contact customer
service.
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Instruction Manual V 2.2 – 01/2010
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6.1 Cleaning the instrument
Switch off the instrument each time before cleaning and pull out the mains plug.
While handling cleaning materials, observe the safety regulations of the manufacturer and the
lab regulations valid in the country of use.
During cleaning, do not allow any liquid to penetrate inside the instrument!
To prevent scratching the surface of the instrument, do not use metallic tools with sharp edges
under any circumstances.
Work surface
• All common laboratory cleaning products suitable for the removal of paraffin (e.g.
Paraguard or xylene substitutes) can be used to clean the work area.
Instrument and exterior surfaces
• If necessary, clean the painted exterior surfaces with a mild household cleaner or soapy
water and wipe with a damp cloth.
• Avoid prolonged contact of organic solvents on the surface of the instrument. Do not
use xylol or acetone on the painted surfaces!
6. Cleaning and maintenance
6.2 Maintenance instructions
Only Leica service technicians are authorized to open the instrument for maintenance and repair work.
Please observe the following points to ensure the instrument’s reliable function over extended periods:
• Clean the instrument with care daily.
• Regularly remove dust from the ventilation slots on the back of the instrument with a
brush or vacuum cleaner.
• Have the instrument inspected at least once a year by an authorized Leica customer
service technician.
• Enter into a service contract at the end of the warranty period. For more information,
contact the relevant Leica customer service organization.
Leica EG115 0 C
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7. Warranty and service
Warranty
Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or
agreed characteristics warranted.
The scope of the warranty is based on the content of the concluded
agreement. The warranty terms of your Leica sales organization or the organization from which you have purchased the contractual product shall
apply exclusively.
Service information
If you require technical service or replacement parts, please contact your
Leica sales representative or dealer who sold the product.
Please provide the following information:
• Model name and serial number of the instrument.
• Location of the instrument and name of the person to contact.
• Reason for the service call.
• Date of delivery.
Decommissioning and disposal
The instrument or parts of the instrument must be disposed of in compliance with the local laws.
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Instruction Manual V 2.2 – 01/2010
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