Leica ASP300 User Manual
Instruction manual
Leica ASP300
V1.3 English - 12/2003
Always keep this manual together with the instrument.
Read this instruction manual carefully
before working with the instrument.
Leica ASP300
Advanced Smart Processor
Automated vacuum
tissue processor
NOTE
The information, numerical data, notes and
value judgments contained in this manual represent the current state of scientific knowledge and state-of-the-art technology.
We are under no obligation to update the
present manual according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of
this manual.
For erroneous statements, drawings, technical illustrations etc. contained in this manual
we exclude liability as far as permissible according to the national legal system applicable
in each individual case. In particular, no liability whatsoever is accepted for any financial
loss or consequential damage caused by or
related to compliance with statements or other
information in this manual.
Statements, drawings, illustrations and other
information as regards contents or technical
details of the present manual are not to be
considered as warranted characteristics of
our products.
These are determined only by the contract
provisions agreed between ourselves and our
customers.
Leica reserves the right to change technical
specifications as well as manufacturing processes without prior notice. Only in this way is
it possible to continuously improve the technology and manufacturing techniques used in
our products.
This document is protected under copyright
laws. Any copyrights of this document are retained by Leica Microsystems Nussloch
GmbH.
Any reproduction of text and illustrations (or of
any parts thereof) by means of print, photocopy, microfiche, web cam or other methods –
including any electronic systems and media –
requires express prior permission in writing by
Leica Microsystems Nussloch GmbH.
© Leica Microsystems Nussloch GmbH
Published by:
Leica Microsystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Telephone: +49 (0)6224 143-0
Fax: +49 (0)6224 143-200
Internet: http://www.histo-solutions.com
Leica ASP300
3
Table of contents
1. Important notes............................................................................................................................. 6
1.1 Symbols used in this manual................................................................................................... 6
1.2 Designated use .......................................................................................................................... 6
2. Safety .............................................................................................................................................. 7
2.1 Safety instructions..................................................................................................................... 7
2.2 Warnings...................................................................................................................................... 8
2.3 Integrated safety devices...................................................................................................... 10
3. Instrument components and specifications .......................................................................... 11
3.1 Specific instrument options................................................................................................... 11
3.2 Overview - instrument components .................................................................................... 12
3.3 Standard delivery - packing list............................................................................................ 14
3.4 Technical data ......................................................................................................................... 15
3.5 Compatible reagents ............................................................................................................... 18
4. Setting up the instrument .......................................................................................................... 19
4.1 Site requirements..................................................................................................................... 19
4.2 Electrical connection.............................................................................................................. 19
4.3 Installing the accessories ..................................................................................................... 21
4.4 Setting up the data connections.......................................................................................... 22
4.5 Alarm functions ........................................................................................................................ 23
4.6 Switching the instrument on ................................................................................................. 24
4.7 Touch screen functions......................................................................................................... 26
4.7.1 User interface - overview ...................................................................................................... 26
4.7.2. Access control ......................................................................................................................... 27
4.8 Check list for first operation (at supervisor access level).............................................. 28
5. Operation...................................................................................................................................... 29
5.1 Reagent handling..................................................................................................................... 29
5.1.1 Fill/drain reagents (other than wax) .................................................................................... 29
5.1.2 Changing the wax.................................................................................................................... 31
5.2 Setting up the instrument parameters................................................................................ 34
5.2.1 System setup............................................................................................................................. 34
5.2.2 Editing the reagent list ............................................................................................................ 37
5.2.3 Viewing the program list......................................................................................................... 40
5.2.4 Adding and/or modifying programs .................................................................................... 41
5.2.5 Favorites (maximum of 5 programs) .................................................................................... 44
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Instruction manual V 1.3 – 12/2003
Table of contents
5.2.6 Editing the stations .................................................................................................................. 46
5.2.7 Reagent groups........................................................................................................................ 47
5.3 Programs.................................................................................................................................... 48
5.3.1 Editing a selected program ................................................................................................... 49
5.3.2 Starting a program................................................................................................................... 51
5.4 Reagent status ......................................................................................................................... 52
5.5 System Monitor......................................................................................................................... 53
5.6 On-line help ............................................................................................................................... 54
5.7 Configuring a printer (optional)............................................................................................. 55
6. Trouble shooting ........................................................................................................................ 56
6.1 General ....................................................................................................................................... 56
6.2 Power failure ............................................................................................................................. 56
6.3 Trouble shooting...................................................................................................................... 57
6.4 Typical fill or drain problems ................................................................................................. 58
7. Cleaning and maintenance ......................................................................................................59
7.1 Clean programs ........................................................................................................................ 59
7.1.1 Retort clean programs............................................................................................................ 59
7.1.2 Wax clean programs .............................................................................................................. 61
7.1.3 Smart clean ............................................................................................................................... 62
7.2 General cleaning steps .......................................................................................................... 63
7.3.1 Daily cleaning and maintenance ......................................................................................... 65
7.3.2 Periodic cleaning and maintenance................................................................................... 66
7.4 Preventative maintenance schedule ................................................................................. 67
8. Warranty and service ................................................................................................................ 69
Leica ASP300
5
1. Important notes
1.1 Symbols used in this manual and their meaning
Warnings
appear in a grey box and are marked
by a warning triangle .
Notes,
i.e. important user information appear
in a grey box and are marked by an
information symbol .
Flammable solvents and reagents are
marked with this symbol.
Instrument surfaces which become
hot during operation are marked with
this symbol.
1.2 Designated use
The Leica ASP300 is an automated tissue processor for the following laboratory applications:
• fixation
• dehydration
• paraffin wax infiltration
of histological tissue specimens.
The Leica ASP300 must be operated exclusively with the reagents listed in
Chapter 3.5 - “Compatible reagents“.
Any other use of the instrument
is considered improper!
(5)
ENTER
6
Figures in brackets refer to item nos.
in drawings.
Function keys to be pressed on the
instrument touch screen are written
in bold-print capital letters.
Instruction Manual V 1.3 – 12/2003
Make sure to comply with the safety instructions and warnings in this chapter.
Make sure to read these instructions, even if you are already familiar with the operation and
use of other Leica products.
2.1 Safety instructions
2. Safety
This instruction manual includes important information related to the operating safety and
maintenance of the instrument and it is an important part of the product.
If additional requirements on accident
prevention and environmental protection exist in the country of operation,
this instruction manual must be
supplemented by appropriate instructions to ensure compliance with such
requirements.
Instrument type
All information provided in this manual applies
only to the instrument type indicated on the title
page.
A name plate indicating the instrument serial
number is attached to the back of the instrument.
This instrument has been built and tested in
accordance with the following safety regulations on electrical measuring, control, regulating and laboratory devices:
• DIN EN ISO 9001
• EN 61010-1
• EN 61010-2
• EN 61326-1 and EN 61326-2
• EN 61000-3-2 and EN 6100-3-3.
The protective devices on both instrument and accessories may neither
be removed nor modified.
Only authorized and qualified service
personnel may repair the instrument
and access the instrument’s internal
components.
Leica ASP300
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2. Safety
2.2 Warnings
The safety devices installed in this instrument by the manufacturer only constitute the basis
for accident prevention.
Primarily responsible for accident-free operation is above all the institution which owns the
instrument and, in addition, the designated personnel who operates, services or repairs the
instrument.
To ensure trouble-free operation of the instrument, make sure to comply with the following
instructions and warnings.
Transport and installation
Warnings and instructions on transport and installation of the instrument can be found on a
separate information sheet attached to the outside of the instrument’s shipping crate.
Warnings - reagent handling
To avoid damage to the instrument, only use the reagents listed in chapter 3.5!
When handling reagents, follow the instructions of the manufacturer and make sure all
laboratory regulations in force in your country are complied with.
Dispose of waste solvents with care according to local regulations and the waste management policy of the company or institution.
Use caution when handling hot, molten paraffin.
Do not touch paraffin stations and retort walls – they can be very hot.
Do not clean the reagent bottles in an automatic dishwasher.
Material safety data sheets can be obtained from the supplier of the chemicals.
They are also available on the Internet:
http://www.msdsonline.com
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Instruction manual V 1.3 – 12/2003
Warnings - cleaning and maintenance
Prior to each maintenance switch the instrument off and disconnect mains power.
Do not clean the instrument with solvents containing acetone or xylene. No liquid may be
spilled into the internal components of the instrument.
Empty the condensate bottle at regular intervals.
Warnings - working with the instrument
The Leica ASP300 may only be operated by trained laboratory personnel and as per the
present instruction manual.
In case of emergency switch off mains (item no. 5 in Fig. 3) at the rear of the instrument and
unplug the power cord.
The instrument must be installed so that the mains switch at the rear of the instrument can
easily be accessed at any time.
Always press PAUSE before opening the retort lid during processing to allow the retort to
vent.
2. Safety
Do not remove the wax drain hose or the remote fill/drain hose until the fill or drain has been
entirely completed.
After refilling / replacing the reagent bottles ensure that the lids are tight.
The bottles must be properly pushed home into the connection manifolds at the rear inner
wall of the reagent module.
Fixatives containing mercuric salts, acetic or picric acid will corrode metallic components
in the instrument.
After each paraffin step a retort clean cycle must be run.
Leica ASP300
9
2. Safety
2.3 Integrated safety devices
The instrument is equipped with the following protection features:
• overpressure protection,
• protection against excessive vacuum,
• overcurrent protection,
• overheating protection.
Overpressure protection
The design of the pressure system ensures that no dangerous levels of
pressure can build up.
Protection against excessive vacuum
The vacuum system is not capable of generating a dangerous vacuum
condition.
Overcurrent protection
Overheating protection
10
Overcurrent conditions are protected against by both the mains fuse
and the separate heating power fuses.
The design of the heating system ensures that no dangerous overheating conditions can occur.
Instruction manual V 1.3 – 12/2003
3.1 Specific instrument options
• Reagent Management System (RMS).
• The specimen cassette baskets may be used with or without cassette dividers and spring separators designed to separate cassettes
and improve fluid penetration.
• In-process fluid recirculation (‘tidal agitation’) for continuous and efficient mixing of fluids.
• Remote fill/drain system for reagents.
• Remote fill/drain system for paraffin.
• Automatic optical fluid level sensor.
• Active paraffin wax solvent removal feature.
• Magnetic stirrer.
• Programmable program start time delay.
• Programmable finish time for processing programs.
• 3-step retort drain feature.
• Specimen processing under pressure, vacuum, alternating pressure
/ vacuum cycles or under ambient pressure.
• User-programmable clean cycles.
3. Instrument components and specifications
Leica ASP300
11
3. Instrument components and specifications
3.2 Overview - instrument components
3
23
22
5
21
20
13
17
18
4
6
1
10 9
7
12 11 8
19
12
2
15
14
16
Fig. 1
Instruction manual V 1.3 – 12/2003
3. Instrument components and specifications
Overview - Instrument components
1 - Basic instrument - processor module
2 - Basic instrument - reagent module
3 - Wax bath lids
4 - Retort lid
5 - Touch screen
6 - Cover flap of instrument console
7 - Instrument console comprising:
8 - Printer port
9 - Local alarm connection
10 - Remote alarm connection
11 - Floppy disk drive
12 - Serial port
13 - Activated carbon filter
14 - Condensate bottle
15 - Reagent bottle (13 units)
16 - Drip tray
17 - Wax drain connection
18 - Remote drain connection
19 - Retort
20 - Wax baths
21 - Specimen basket
22 - Lid for specimen basket
23 - Splash protector
Instrument components and accessories
The processor module comprises three paraffin wax baths and the processing retort.
Three baskets (21) contain the cassettes.
Each basket can hold up to 300 cassettes.
All processing occurs in the stainless steel re-
tort under the pressure, vacuum and temperature conditions selected.
The reagent bottles are stored in the reagent
cabinet.
Leica ASP300
13
3. Instrument components and specifications
3.3 Standard delivery - packing list
Leica ASP300 basic standard delivery:
1 Leica ASP300 - Basic instrument 0476 34100
1 Accessory kit consisting of: 0476 35929
- 1 Basket hook
0476 34713
- 3 Specimen baskets, assy., including lid, dividers, springs and handle 0476 34193
(accessories for maximum and optimum usage of basket space)
- 1 Reagent bottle, plastic 0476 34274
- 2 Activated carbon filters, assy. 0476 34150
- 1 Remote fill/drain hose, assy. with special connecting device
for remote filling / draining of processing retort 0476 34716
- 1 Paraffin drain hose 0476 34721
- 1 Paraffin wax scraper, plastic 0476 35923
- 1 Lubricant ‘Molykote 111’, for valves and O-rings, 100 g 0336 35460
- 1 Jumper cable - mains 0411 34604
- 1 Maintenance kit (2 spare lids, 9 O-rings) 0476 35921
- 1 Stirrer bar, assy. 0476 34202
- 2 Sets of labels for reagent bottles, each set containing 15 labels 0476 35792
- 1 Splash protector 0476 34770
1 Set of power cords:
- 1 Power cord D 0411 13558
- 1 Power cord UK ST-BU F-5A 0411 27822
13 Reagent bottles, plastic (included in the instrument) 0476 34274
1 Condensate bottle, plastic (included in the instrument) 0476 34278
1 Service kit for pump (included in the instrument) 0476 35922
1 Drip tray (included in the instrument) 0476 34145
1 Cover sheet for overflow (included in the instrument) 0476 34852
1 Console cover (mounted to the instrument) 0476 34162
1 3,5 Diskette, empty (taped to the back of console cover)
1 Instruction manual Leica ASP300 multilingual 0704 37102
1 Demo-Program, CD-Rom
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Instruction manual V 1.3 – 12/2003
3. Instrument components and specifications
3.4 Technical data
Nominal voltage: Two factory-preset voltage settings
(not user-adjustable):
100 to 120 V + 10% or
230 to 240 V + 10%
Nominal frequency: 50 to 60 Hz
Main fuses: 2 fast-blowing melting fuses, 20 x 5 mm, UL-listed
Order no.
• 100 to 120 V; F 10 A 250 VAC 69411002
• 230 to 240 V; F 5 A 250 VAC 69411000
Nominal power: 1,000 VA
Dimensions (W x D x H), in mm: 595 x 680 x 1,325
Dry weight, unpacked: approx. 140 kg
Weight, packed: 220 kg
Operating temperature range: 15 °C to 35 °C
Relative humidity: 10% to 80% - non-condensing
IEC 1010 classification: Protective Class 1
Pollution Degree 2
Overvoltage Installation Category II:
• 800 V impulse (120 V systems)
• 1500 V impulse (240 V systems)
Maximum operating altitude: maximum 2,500 m
Local / remote alarm relays: 30 V DC, maximum 2 A
2 connections:
both single voltage-free, change-over contacts
(can be operated as both normally-open and
normally-closed connections)
Paraffin wax baths
Number of baths: 3
Volume (liters): 4.3 l per bath
Melt time: approx. 10 hrs
Temperature: 40 to 65 °C
Temperature accuracy:
+ 1 °C
Leica ASP300
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3. Instrument components and specifications
Retort
Capacity: max. 300 cassettes
Reagent volume (in liters): 4.3
Temperature (paraffin): 40 to 65 °C
Temperature (processing reagents): ambient or 35 to 55
Temperature (cleaning reagents): 50 to 65 °C
Temperature accuracy:
+ 1 K
Filling time: approx. 90 sec
Drain time : approx. 80, 120, 140 sec (adjustable)
Impregnation vacuum: -70 kPa (g)
Impregnation pressure: 35 kPa (g)
Fill vacuum: -70 kPa (g)
Drain pressure: 35 kPa (g)
General
Reagent bottles 10
Cleaning solution bottles: 3
Maximum bottle volume: 4.3 l
Printer: Optional accessory
Pretest check: ON/OFF
Fluid level sensor: ON/OFF
Recirculation (pump in/out): ON/OFF
(a) Time before 1st cycle: 12 min
(b) Time between cycles: 20 min
o
C
System setup
Password status: Supervisor / User
Type of password: alphanumeric, freely selectable
Reagent Management Status: ON/OFF
Software access control (‘Lock Mode’): ON/OFF
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Instruction manual V 1.3 – 12/2003
3.4 Technical Data (continued)
Hardware and Software:
• Large color liquid crystal display (LCD) and touchscreen.
• User-friendly, intelligent software.
• 3,5“ floppy drive and printer port.
• Alarm system with two remote alarm sockets.
• Password-protected instrument supervisor mode.
• Multiple specimen protection system.
Capacities:
• 15 programs of up to 10 reagent and 3 paraffin wax steps each.
• Time per program step: 0 to 99 hours , 59 minutes.
• Delay time: max. 7 days.
• Up to 300 cassettes can be processed simultaneously.
• Four user-programmable retort clean programs.
• Wax clean program.
• 10 Reagent bottles.
• 3 Wax baths.
• 3 Cleaning solution bottles.
• 1 Condensate bottle.
• Reagent temperature adjustable from 35°C to 55°C
or ambient.
• Paraffin temperature adjustable from 40°C to 65°C.
• Choice of three retort drain rates of 80, 120 and 140 seconds.
• Up to 100 reagent names in memory.
3. Instrument components and specifications
Leica ASP300
17
3. Instrument components and specifications
3.5 Compatible reagents
Fixatives
1. Formalin, buffered or unbuffered
2. Formalin saline
Dehydration
1. Ethanol 4. Butanol
2. Isopropanol 5. Industrial Methylated Spirits
3. Methanol
Clearing
1. Xylen (recommended)
2. Toluol
*
3. Chloroform *
Paraffin wax
1. Paraffin
* Prior to using these reagents, contact
your Leica representative or Leica directly for information on necessary protective measures. Using these reagents
will require an increased number of maintenance interventions by Leica Technical Service.
18
Caution:
Reagents other than those listed here may damage some components of the instrument.
Do not use acetone, benzene or trichlorethane in the instrument.
Fixatives containing mercuric salts, acetic or picric acid will
corrode metallic components in the instrument and shorten instrument life.
If you choose to work with such fixatives, it is essential to perform a clean cycle which contains multiple water rinses each
time after use, to minimize damage.
In addition, we recommend frequent and regular preventive
maintenance by the Leica Technical Service.
Instruction manual V 1.3 – 12/2003
4.1 Site requirements
Moving the instrument
4. Setting up the instrument
• The instrument needs approx. 650 x 700 mm floor space.
• Practically vibration-free floor.
• Room temperature constantly between +15 °C and +35 °C.
• Relative humidity maximum 80%, non-condensing.
• Avoid vibrations, direct sunlight and heavy variation in temperature.
The chemicals to be used in the Leica ASP300 are both flammable and noxious. Therefore, the instrument must be set up in
a well ventilated area, free from any ignition sources.
The room where the instrument is installed is not suitable for
people to stay in for longer periods of time, unless it is
equipped with a fume extraction system.
Do not operate the instrument in rooms with explosion hazard.
The instrument must be installed such that the mains switch at
the rear of the instrument (item no. 5 in Fig. 3) can easily be accessed at any time.
After the instrument has been unpacked (see unpacking instructions
attached to outside of transport crate) it must be touched only at the
points marked with dots ‘ ‘ for transport to its final site of installation.
Once the instrument has been installed there, the brakes on the instrument rollers must be locked.
4.2 Electrical connection
• Check the voltage label (Fig. 2) on the rear of the instrument to ensure
Leica ASP300
that the instrument is set to the correct voltage range.
Fig. 2
The power supply voltage for the instrument is factory-preset
and CANNOT be altered by the user.
19
Connecting the instrument to mains
The instrument MUST be connected to an earthed mains power
outlet socket.
It is recommended that the ASP300 be plugged into a wall socket
that has Ground Fault Circuit Interruption (GFCI) protection - as
an additional electrical safeguard.
Make sure to use the appropriate mains cable for the local voltage supply (wall outlet).
• Connect mains cable (6) to mains power supply socket (1) (see Fig. 3).
• Jumper cable (3) connects the mains power supply output (2) to the
electronics module input (4).
• Switch ON/OFF switch (5) ON.
• Switch ON/STOP switch at the right of the instrument ON.
Electrical
connections at the
1
5
rear panel of the
instrument
4. Setting up the instrument
6
20
4
2
3
Cables
connected
Fig. 3
correctly
Important:!
Specification for connection (2) is as follows:
100 - 120 V or 230 - 240 V, max. 200VA.
Instruction manual V 1.3 – 12/2003
4. Setting up the instrument
6
4.3 Installing the accessories
• Unpack the activated carbon filter and insert it into the instrument (see Fig. 4). Make
sure to insert the filter with the correct side
facing upwards. See label (6) at the front of
7
the filter - the arrow must point upwards.
• Unpack the magnetic stirrer and insert into
the retort.
Fig. 4
• Install the console flap (covers floppy drive
and ports).
l Prior to filling the bottles with reagent for the
first time, check the connections. If necessary, tighten manually.
Remote fill / drain hose
• Connect the remote fill / drain hose as
shown in Fig. 5.
8
• Important!
When inserting the hose into the fill/drain
connection (item 7 in Fig. 5) the connecting
device (8) of the hose must lock with an audible click.
Leica ASP300
7
Fig. 5
21
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