Leica ASP 200 S V1.1 User Manual
Operating Manual
Leica ASP200 S
V1.1 English - 03/2009
Always keep this manual together with the instrument.
Read this instruction manual carefully before working
with the instrument.
Leica ASP200 S
Advanced Smart Processor
Automated vacuum
tissue processor
NOTE
The information, numerical data, notes and value
judgments contained in this manual represent
the current state of scientific knowledge and
state-of-the-art technology as we understand it
following thorough investigation in this field.
We are under no obligation to update the present
manual according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.
For erroneous statements, drawings, technical
illustrations etc. contained in this manual we
exclude liability as far as permissible according
to the national legal system applicable in each
individual case. In particular, no liability whatsoever is accepted for any financial loss or consequential damage caused by or related to compliance with statements or other information in this
manual.
Statements, drawings, illustrations and other
information as regards contents or technical
details of the present manual are not to be
considered as warranted characteristics of our
products.
These are determined only by the contract provisions agreed between ourselves and our customers.
Leica reserves the right to change technical
specifications as well as manufacturing processes without prior notice. Only in this way is it possible to continuously improve the technology and
manufacturing techniques used in our products.
This document is protected under copyright
laws. Any copyrights of this document are
retained by Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of
any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – including
any electronic systems and media – requires
express prior permission in writing by
Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the name plate at
the back of the instrument.
© Leica Biosystems Nussloch GmbH
Published by:
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Telephone: +49 (0)6224 143-0
Fax: +49 (0)6224 143-200
Internet: http://www.histo-solutions.de
Leica ASP200 S
3
Table of Contents
1. Important information ................................................................................................................. 6
2. Safety ..............................................................................................................................................7
2.1 Safety instructions .................................................................................................................................... 7
2.2 Warnings .................................................................................................................................................... 7
2.3 Integrated safety devices ...................................................................................................................... 11
3. Instrument components and specifications ......................................................................... 12
3.1 Overview - instrument components..................................................................................................... 12
3.2 Specific instrument options .................................................................................................................. 14
3.3 Standard delivery - packing list ............................................................................................................ 15
3.4 Technical Data ........................................................................................................................................ 16
3.5 Compatible reagents .............................................................................................................................. 19
4. Setting up the instrument ......................................................................................................... 20
4.1 Site requirements ................................................................................................................................... 20
4.2 Electrical connection ............................................................................................................................. 20
4.3 Installing the accessories ..................................................................................................................... 21
4.4 Setting up the data connections .......................................................................................................... 23
4.5 Anti-reflection clip – Installation Instructions.................................................................................... 24
4.6 Alarm functions ....................................................................................................................................... 25
4.7 Switching the instrument on ................................................................................................................. 26
4.8 Touchscreen functions .......................................................................................................................... 28
4.9 Checklist for first operation ................................................................................................................... 29
4.10 Switching of the instrument .................................................................................................................. 30
5. Operation ..................................................................................................................................... 31
5.1 Setting up the instrument parameters ................................................................................................. 31
5.1.1 System setup ........................................................................................................................................... 31
5.1.2 Access levels .......................................................................................................................................... 34
5.1.2 INSTALLATION menu............................................................................................................................. 36
5.1.3 Editing the reagent list ........................................................................................................................... 39
5.1.4 Viewing the program list........................................................................................................................ 42
5.1.5 Adding and/or modifying programs ..................................................................................................... 43
5.1.6 Favorites ................................................................................................................................................... 46
5.1.7 Editing the stations ................................................................................................................................. 48
5.1.8 Reagent groups ....................................................................................................................................... 49
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Operating Manual V 1.1 – 03/2009
Table of Contents
5.2 Reagent handling .................................................................................................................................... 50
5.2.1 Fill/drain reagents (other than wax)..................................................................................................... 50
5.2.2 Changing the wax ................................................................................................................................... 52
5.3 Running programs .................................................................................................................................. 55
5.3.1 Editing a selected program ................................................................................................................... 56
5.3.2 Starting a program.................................................................................................................................. 58
5.4 Reagent status ........................................................................................................................................ 59
5.5 System Monitor ....................................................................................................................................... 60
5.6 On-line help.............................................................................................................................................. 61
6. Solutions...................................................................................................................................... 62
6.1 General ..................................................................................................................................................... 62
6.2 Power failure ........................................................................................................................................... 62
6.3 Troubleshooting ...................................................................................................................................... 63
6.4 Typical fill or drain problems................................................................................................................. 64
7. Cleaning and Maintenance ..................................................................................................... 65
7.1 Clean programs ....................................................................................................................................... 65
7.1.1 Retort clean programs ........................................................................................................................... 65
7.1.2 Wax clean program ................................................................................................................................ 67
7.1.3 Smart Clean ............................................................................................................................................. 68
7.2 General cleaning steps .......................................................................................................................... 69
7.3.1 Daily cleaning and maintenance.......................................................................................................... 71
7.3.2 Periodic cleaning and maintenance.................................................................................................... 73
7.4 Preventative maintenance schedule ................................................................................................... 74
8. Warranty and Service ............................................................................................................... 76
9. EC Declaration of Conformity ..................................................................................................77
Leica ASP200 S
5
1. Important information
Symbols used in this manual
Warnings
appear in a gray box and are
(5)
ENTER
marked by a warning triangle
Notes,
i.e. important user information appear in a gray box and are marked
by an information symbol
Flammable solvents and reagents
are marked with this symbol.
Instrument surfaces which become
hot during operation are marked
with this symbol.
Avoid direct contact with these surfaces - they may cause burns.
Figures in brackets refer to item
nos. in drawings.
Function keys to be pressed on the
instrument touch screen are written
in bold-print capital letters.
.
.
Qualification of personnel
• The Leica ASP200 S may be operated only by
trained laboratory personnel.
•
The instrument may be operated only according to the instructions contained in this manual.
Designated use
The instrument has been designed so that it is
safe to use by the operator as well as for processing specimens – provided that it is operated
according to the present instruction manual.
The Leica ASP200S is a modular automated
tissue processor designed for the following laboratory applications:
• fixation,
• dehydration
• paraffin wax infiltration of histological
tissue specimens.
The Leica ASP200 S must be operated exclusively
with the reagents listed
in chapter 3.5 - "Compatible reagents" .
Any other use of the instrument is
considered improper!
Failure to adhere to these instructions may result in an accident,
personal injury, damage to the
instrument or accessory equipment.
Instrument type
All information provided in this manual applies
only to the instrument type indicated on the title
page.
A name plate
indicating the
instrument
serial number
is attached to
the back of the
instrument.
Fig. 1
6
Operating Manual V 1.1 – 03/2009
Make sure to comply with the safety instructions and warnings in this chapter.
Make sure to read these instructions, even if you are already familiar with the operation
and use of other Leica products.
2.1 Safety instructions
This instruction manual includes important information related to the operating safety and maintenance of the instrument and it is an important
part of the product.
2. Safety
This instrument has been built and tested in accordance with the safety regulations on electrical measuring, control, regulating and laboratory
devices.
In order to maintain this condition and to ensure
safe operation the operator must comply with the
instructions and warning contained in this
instruction manual.
If additional requirements on accident prevention and environmental
protection exist in the country of operation, this instruction manual
must be supplemented by appropriate instructions to ensure compliance with such requirements.
The protective devices on both instrument and accessories may neither be removed nor
modified. Only authorized and qualified service personnel may repair the instrument and
access the instrument’s internal components.
For current information on applicable
standards, please refer to the instrument’s CE declaration and visit:
http://www.histo-solutions.com
2.2 Warnings
The safety devices installed in this instrument by the manufacturer only constitute the basis for
accident prevention. Primarily responsible for accident-free operation is above all the institution
which owns the instrument and, in addition, the designated personnel who operates, services or
repairs the instrument.
To ensure trouble-free operation of the instrument, make sure to comply with the following
instructions and warnings.
Leica ASP200 S
7
2. Safety
Warnings - Markings on the instrument itself
Markings on the instrument showing the warning triangle indicate that the correct
operating instructions (as defined in this manual) must be followed when operating or
replacing the item marked. Failure to adhere to these instructions may result in an accident, personal injury, damage to the instrument or accessory equipment.
Some instrument surfaces become hot during operation.
They are marked with this warning label. Touching these surfaces may cause
burns.
Transport and Installation
The instrument may only be transported in an upright position.
Follow the unpacking instructions carefully to avoid damage to the instrument!
A cleaning must be performed prior to each transport, during which the instrument may
be shaken, tilted or lifted. Severe internal damage may otherwise occur.
The instrument MUST be connected to an earthed mains power outlet socket. The instrument must not be connected to an extension cord without protective earth conductor.
Ensure uniform current supply according the to set voltage!
The set voltage CANNOT be changed by the user.
Severe damage may occur if the instrument is connected to a power supply voltage other
than that to which it was originally set.
The instrument must be set up in a well ventilated area, free from any ignition sources.
The chemicals to be used in the Leica ASP200 S are both flammable and noxious.
Do not operate the instrument in rooms with explosion hazard.
If there is a significant difference in temperature between the warehousing and the installation site of the instrument and if at the same time there is a high air humidity level,
condensation water may form. In this case, a waiting period of at least two hours must be
observed before the instrument is switched on. Failure to adhere to this waiting period
may result in damage to the instrument. Failure to adhere to this waiting period may result in damage to the instrument.
8
Operating Manual V 1.1 – 03/2009
Warnings - Operating the instrument
The Leica ASP200S may only be operated by trained laboratory personnel, according to
its designated use and per the present instruction manual.
The ON/STOP switch at the side of the instrument may be used in an emergency to stop
the instrument during operation.
Always press PAUSE before opening the retort lid during processing to allow the retort to
vent.
Do not remove the wax drain hose or the remote fill/drain hose until the fill or drain has
been completed, as pressurized air is used to clear the hose after each fill / drain.
After refilling / replacing the reagent bottles ensure that the lids are tight.
The bottles must be properly pushed home into the connection manifolds at the rear inner
wall of the reagent module.
Failure to correctly plug the reagent bottles into the manifold will cause an interruption
to the processing run and may result in spilling of reagents.
Fixatives containing mercuric salts, acetic or picric acid will corrode metallic components in the instrument.
2. Safety
After each paraffin step a retort clean cycle must be run.
Material safety data sheets can be obtained from the supplier of the chemicals.
They are also available on the Internet:
http://www.msdsonline.com
Leica ASP200 S
9
2. Safety
Warnings -handling reagents
Be careful when handling solvents!
Always wear rubber gloves and safety goggles when handling the chemicals used in this
instrument.
Reagents used for tissue infiltration can be both toxic and/or flammable.
To avoid damaging the instrument only use those reagents that are listed in chapter 3.5!
Do not use acetone, benzene or trichlorethane in the instrument!
Use caution when handling paraffin wax or removing baskets – molten paraffin is hot and
may cause burns.
Also, avoid personal contact with paraffin stations and retort walls – they can be very hot
as well.
Dispose of waste solvents with care according to local regulations and the waste management policy of the company or institution.
Do not clean reagent bottles in an automatic dishwasher – they are NOT dishwasherproof.
Warnings - Cleaning and maintenance
Prior to each maintenance and/or cleaning, switch the instrument off and disconnect
mains power.
Do not clean the instrument with solvents containing acetone or xylene. No liquid may be
spilled into the internal components of the instrument – neither during operation nor
during cleaning.
When working with cleaning detergents, comply with all safety instructions by the manufacturer of the product and the laboratory management policy.
The condensate bottle should be inspected at least once every week and, if necessary,
emptied.
10
Operating Manual V 1.1 – 03/2009
2.3 Integrated safety devices
In the case of power failures or other processing problems the Leica
ASP200S incorporates specimen protection features such as a fluid level
sensor and sophisticated software controls which ensure that processing
can be completed successfully without damage to the tissue specimens.
Overpressure protection
• When power is off the air pump and air valves default to a safe condition
(retort vented, no pressure generation).
• If the microprocessor control fails to stop the air pump at the right
moment during retort pressurizing, a separate electronic hardware
circuit will cut pump power.
• In addition, there is a safety relief valve that vents all excess air pump
output to atmosphere.
Overcurrent protection
• Overcurrent conditions are protected against by both the mains fuse and
the separate heating power fuses.
Overheating protection
An error is indicated and all heating is stopped by the microprocessor
control if the instrument detects any of the following conditions:
• abnormally high temperature (>75 °C)
• contradictory results of the temperature sensors
• failure of one or more heating power control components
• If the microprocessor fails to interrupt heating power, independent
temperature limiting hardware circuits limit the temperature rise to a
safe level.
• If the temperature limiting circuits malfunction, an independent hardware thermal fuse circuit cuts power to the heating elements.
2. Safety
Over vacuum protection
Overcurrent protection
Leica ASP200 S
• The vacuum system is not capable of generating a dangerous vacuum
condition.
• Overcurrent conditions are protected against by both the mains fuse and
the separate heating power fuses.
11
3. Instrument components and specifications
3.1 Overview - instrument components
24
22
23
20
21
13
3
4
6
1
10 9
7
5
12 11 8
17
18
19
25
2
12
14
15
16
Operating Manual V 1.1 – 03/2009
Fig. 2
3. Instrument components and specifications
Overview - instrument components
1 - Basic instrument - processor module
2 - Basic instrument - reagent module
3 - Wax bath lids
4 - Retort lid
5 - Touch screen
6 - Cover flap of the instrument console
7 - Instrument console comprising:
8 - Printer port
9 - Local alarm connection
10 - Remote alarm connection
11 - Floppy disk drive
12 - Serial port
13 - Activated carbon filter
14 - Condensate bottle
15 - Reagent bottle (13 units)
16 - Drip tray
17 - Wax drain connection
18 - Remote drain connection
19 - Retort
20 - Wax baths
21 - Specimen basket
22 - Lid for specimen basket
23 - Specimen basket carrier
24 - Splash protection
25 - Displacement block
Instrument components and accessories
The processor module contains three paraffin
wax baths, a processing retort, and also a touch
screen with integrated key symbols as well as
the electronic components.
The cassettes are held in three baskets (21), each
of which has a capacity of up to 100 cassettes.
All processing occurs in the stainless steel retort
under the pressure, vacuum and temperature
conditions selected.
The quick-connect reagent bottles are stored in
the reagent cabinet.
Leica ASP200 S
13
3. Instrument components and specifications
3.2 Specific instrument options
• Reagent Management System option (RMS) that displays the usage
(length and frequency of use) of each reagent and also allows automatic
ordering of reagent sequence, thus eliminating the need to physically
move reagent bottles. After renewal of one or more reagents in a sequence, the RMS automatically uses the reagents in order of increasing
cleanliness.
• In-process fluid recirculation ("tidal agitation") for continuous and
efficient mixing of fluids.
• Remote fill/drain system – can drain and refill reagent bottles from bulk
containers via the retort and a hose plugged into the processor module
while the user is fully protected against coming into contact with
reagents.
• Remote wax bath fill/drain feature.
• Optical fluid level sensor.
• Active paraffin wax solvent removal feature that increases the life of the
wax by extracting and condensing contaminating solvents.
• Magnetic stirrer – to gently circulate reagents, ensuring uniform reagent
temperature.
• Programmable finish time for processing programs.
• Three-step retort drain feature (adjustable) minimizes reagent carryover.
• Specimen processing under pressure, vacuum, alternating pressure /
vacuum cycles or under ambient pressure.
• Four user-programmable clean programs. The clean programs
automatically skip any steps not required to complete the clean.
14
Operating Manual V 1.1 – 03/2009
3. Instrument components and specifications
3.3 Standard delivery - packing list
The basic version of the Leica ASP200 S includes the following parts: Part no.
1 Leica ASP200 Basic Instrument
1 Set of power cords
230-240 V version:
1 Mains cable "D" 14 0411 13558
1 Mains cable "UK" ST-BU F-5A 14 0411 27822
100-120 V version:
1 Mains cable "USA-C-J" 14 0411 13559
1 Jumper cable - mains 14 0411 34604
1 ASP300 S basket set, consisting of:
1 Basket carrier 14 0480 43578
2 Specimen baskets with integrated spacers 14 0476 43569
1 Cover for first basket 14 0476 43362
1 Displacement block 14 0480 37127
1 Remote fill/drain hose 14 0476 34716
1 Paraffin drain hose 14 0476 34721
1 Funnel 14 0476 43631
1 Lubricant, Molykote 111, for valves and O-rings 14 0336 35460
2 Activated carbon filters, assy. 14 0476 34150
1 Magnetic stirrer, complete 14 0476 34202
1 Paraffin wax scraper 14 0476 35923
14 Reagent bottles, plastic (13 in instrument) 14 0476 34274
1 Condensate collector, plastic (in instrument) 14 0476 34278
1 Drip tray (in instrument) 14 0476 34145
1 Splash protection 14 0476 34770
1 Maintenance kit (2 replacement lids, 9 o-rings) 14 0476 35921
1 Maintenance kit for pump (in instrument) 14 0476 35922
2 Sets of labels for reagent bottles, set of 15 each 14 0476 35792
1 Remote alarm plug 14 6844 01005
1 Operating manual for Leica ASP200 S, multi-language 14 0480 80001
1 3,5" HD-diskette, blank, DOS-formatted, (on rear of console cover)
Please check all delivered parts against the packing list and against your order to
verify whether the delivery is complete!
local Leica Sales Office immediately.
Leica ASP200 S
If there is any difference, please contact your
15
3. Instrument components and specifications
3.4 Technical Data
Nominal voltage: Two factory-preset voltages (not user-adjustable):
100 to 120 V or
230 to 240 V
Nominal frequency:
Main fuses: Two fast-blowing melting fuses, 20 x 5 mm, UL-approved
Nominal power: 1000 VA
Dimensions, (W x D x H), in mm: 595 x 680 x 1,325
Dry weight, unpacked: approx. 160 kg
Weight, packed: 220 kg
Operating temperature range: 15 °C to 35 °C
Relative humidity: 10 % to 80 %, non-condensing
IEC 1010 classifications: Protective class 1
Altitude: 2500 m maximum
Local / remote alarm relays: 30 V DC, maximum 2 A
50 to 60 V
• for 100 to 120 V ; F 10 A 250 VAC
• for 230 to 240 V ; F 5 A 250 VAC
Pollution degree 2
Overvoltage installation category II:
• 800 V impulse (120 V systems)
• 1500 V impulse (240 V systems)
2 connections:
Both single voltage-free, changeover contacts
(both Normally-Open and Normally-Closed
connections available)
Paraffin wax baths
Number of baths: 3
Volume (liters): 4.3 l per bath
Melt time: approx. 10 hrs
Temperature: 40 to 65 °C
Temperature accuracy:
+ 1 K
16
Operating Manual V 1.1 – 03/2009
3. Instrument components and specifications
Retort
Capacity: max. 250 cassettes
Reagent volume (in liters): 4.3
Temperature (paraffin): 40 to 65 °C
Temperature (processing reagents): ambient or 35 to 55
Temperature (cleaning reagents): 50 to 65 °C
Temperature accuracy:
+ 1 K
Filling time: approx. 90 sec
Drain time: approx. 80, 120, 140 sec (adjustable)
Impregnation vacuum: -70 kPa (g)
Impregnation pressure: 35 kPa (g)
Fill vacuum: -70 kPa (g)
Drain pressure: 35 kPa (g)
General
Reagent bottles: 10
Cleaning solution bottles: 3
Maximum bottle volume: 4.3 l
Printer: Optional accessory
Pretest check: ON/OFF
Fluid level sensor: ON/OFF
Recirculation (pump in/out): ON/OFF
(a) Time before 1st cycle: 16 min
(b) Time between cycles: 20 min
System setup
Password status: Supervisor / User
Type of password: alphanumeric, freely selectable
Reagent Management Status: ON/OFF
Software access control (lock mode): ON/OFF
°C
Leica ASP200 S
17
3. Instrument components and specifications
3.4 Technical Data (continued)
Hardware and Software:
• Large color Liquid Crystal Display (LCD) and touchscreen.
• User-friendly, intelligent software.
• 3,5" floppy drive and printer port.
• Alarm system with two remote alarm sockets.
• Password-protected instrument supervisor mode.
• Multiple specimen protection system.
Capacities:
• 15 programs that consist of up to 10 reagent and 3 paraffin wax steps.
• Time per program step: 0 to 99 hours, 59 minutes.
• Delay time: max. 7 days.
• Up to 250 cassettes can be processed simultaneously.
• Four user-programmable retort clean programs.
• Wax clean program.
• 10 reagent bottles.
• 3 wax baths.
• 3 cleaning solution bottles.
• 1 condensate bottle.
• Reagent temperature adjustable from 35 °C to 55 °C
or ambient.
• Paraffin temperature adjustable from 40 °C to 65 °C.
• Choice of three retort drain rates of
80, 120 and 140 seconds.
• Up to 100 reagent names in memory.
18
Operating Manual V 1.1 – 03/2009
3. Instrument components and specifications
3.5 Compatible reagents
The following reagents may be used in the Leica ASP200S:
Fixatives
1. Formalin, buffered or unbuffered
2. Formalin saline
Dehydration
1. Ethanol 4. Butanol
2. Isopropanol 5. Industrial Methylated Spirits
3. Methanol
Clearing
1. Xylene
2. Toluol
3. Chloroform *
Paraffin wax
1. Paraffin
*
*
Prior to using these reagents, contact your
Leica representative or Leica directly for in-
formation on necessary protective measures.
Leica ASP200 S
Caution:
Reagents other than those listed here may damage some components of the instrument.
Do not use acetone, benzene or trichlorethane in the instrument.
Fixatives containing mercuric salts, acetic or picric acid will
corrode metallic components in the instrument and shorten
instrument life.
If you choose to work with such fixatives, it is essential to
perform a clean cycle which contains multiple water rinses
each time after use, to minimize damage.
In addition, we recommend frequent and regular preventive
maintenance by the Leica Technical Service.
19
4. Setting up the instrument
4.1 Site requirements
• The instrument must be set up in a clear space
of about 650 x 700 mm on a non-vibrating base.
• Room temperature constantly between +10 °C
and +35 °C.
• Relative humidity maximum 80%, non-condensing.
• Avoid vibrations, direct sunlight and heavy
variation in temperature.
Moving the instrument
4.2 Electrical connection
Caution!
The instrument MUST be connected
to an earthed mains power outlet
socket.
It is recommended that the ASP200S
be plugged into a wall socket that
has Ground Fault Circuit Interruption
(GFCI) protection – as an additional
electrical safeguard.
Make sure to use the appropriate
mains cable for the local voltage
supply (wall outlet).
The chemicals to be used in the
Leica ASP200S are both flammable
and noxious. The Leica ASP200S
must be set up in a well ventilated
area free from any ignition sources.
Do not operate the instrument in
rooms with explosion hazard.
When the instrument has been unpacked (see
Unpacking instructions on the outside of the shipping container), it must be grasped only at the
points indicated by ‘ ‘ to move it to its final setup
location. Once it has reached this position, the
brakes on the instrument rollers must be engaged immediately.
• Check the voltage label (Fig. 3) on the rear of
the instrument to ensure that the instrument
delivered is set to the correct voltage range.
Severe damage may occur if the instrument is connected to a power
supply voltage other than that to
which it was originally set.
The power supply voltage for the instrument is factory preset, and CANNOT be altered by the user.
20
The instrument must be set up so
that the power switch on the rear of
the instrument (
be reached at all times.
item 42 in fig. 4) can
Fig. 3
Operating Manual V 1.1 – 03/2009
4. Setting up the instrument
Connecting the instrument
to mains
41
42
40
43
Illustration on the left
Electrical connections at the
rear panel of the instrument.
Once the instrument has been switched on, the mains
switch (ON/OFF) (42) should always remain in position “ON“.
37
38
Illustration on the right
Correct connection of the
cables
• Connect the mains cable (37) to
the mains power supply socket
(41).
• The jumper cable (38) which is
supplied as part of standard
delivery connects the mains
power supply output (43) to the
electronics module input (40).
Important!
The specification for
Fig. 4
connection (40) is as
follows:
100 - 120 V or
230 - 240 V,
maximum 200VA.
4.3 Installing the accessories
13
29
Leica ASP200 S
18
Fig. 5
• Place the instrument in the definitive installation position.
Activated carbon filter
• Unpack the activated carbon filter (29) and insert it into the instrument (see
Fig. 5).
Make sure to insert the filter with the correct
side facing upwards. See label
(27) at the front of
the filter – the arrow must point
upwards.
21
4. Setting up the instrument
28
Remote fill / drain hose
• Connect the supplied remote fill/drain hose to
the remote drain connection on the front of the
instrument (
see Fig. 6).
• Important!
When inserting the hose into the fill/drain con-
nection (item 18 in
Fig. 6) the connecting de-
vice (28) of the hose must lock with an audible
click.
31
32
18
30
Fig. 6
If bulk containers can be located
next to the instrument during remote
filling and draining, the hose may be
shortened to improve handling and
the fill/drain rate.
If the hose is shortened, a vee-notch
can be cut into the hose end to ensure a clear passage for fluid flow.
Magnetic stirrer
• Unpack the magnetic stirrer (30,
fig. 7) and in-
sert into the retort.
• Important!
Insert the magnetic stirrer so that the convex
side (32) of the rotor blades (31) faces down.
(Fig.
8)
Inserting the stirrer incorrectly will result in
unsatisfactory stirring action.
22
Fig. 7
Operating Manual V 1.1 – 03/2009
4. Setting up the instrument
25
26
Important!
The instrument must not be operated
unless the volume displacement
block is in the retort. Otherwise,
malfunctions may cause damage to
the instrument.
27
25
• Grasp the volume displacement block (25) by
the metal clip (26) and insert into the retort so
that the hole (27) is located above the position
of the magnetic stirrer.
Inserting the Displacement block
Fig. 8
4.4 Setting up the data connections
10
9
8
11
12
6
Printer (optional)
• Lift the flap of the instrument console (6).
• Connect the printer to the printer port (8) in the
console via a standard Centronics cable
(
Fig. 9)
• A suitable printer can be recommended by
your Leica distributor.
• If conformance to electromagnetic interfer-
ence standards is essential, a specially shielded printer cable will be required.
Other connections
• For additional data transfer and storage the
instrument console is equipped with a standard 1.44 MB floppy disc drive (11) and an RS
232 serial interface (12).
Leica ASP200 S
Fig. 9
23
4. Setting up the instrument
4.5 Anti-reflection clip – Installation Instructions
Anti-reflection clip – Function
The anti-reflection clip (Order no. 14 0476 44135) prevents any reflections that might otherwise be emitted from the specimen basket (3, order no. 14 0476 34193) and that could affect the level sensor in the
retort, thus causing malfunctions.
Installing the clip
The anti-reflection clips are intended for
installation on the specimen basket to
prevent reflections of the level sensor.
1
3
• Insert the left lug (2a) of the anti-reflection clip (1)
into the seventh hole (from the left) of the lower oval
openings (Fig. 9).
4
2a
Fig. 9
• Snap the right lug (2b, Fig. 10) into the seventh hole
(from the right) with light pressure.
When inserting or removing the metal
baskets into/from the retort, always do so
carefully so that they do not touch or become caught on the retort wall.
1
4
2b
Fig. 10
Changing the clip
• Once the clip is installed, the handle (4) of the specimen basket cannot be removed, as otherwise the
clip will be bent.
• To remove or replace a clip, all parts that are inside
4
5
the basket (e.g. spiral insert and separating walls)
must be removed from the basket.
• Then, insert a suitable tool (screwdriver 5, Fig. 11)
and pry the clip out from the inside.
24
Fig. 11
Once bent, clips may no longer be used
and must be disposed of. In this case, install a new clip from the standard scope
of delivery.
Operating Manual V 1.1 – 03/2009
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