Leica APPLICATION SUITE RETICULE, LAS RETICULE User Manual

Leica
Application
Suite
Reticule
Reticules Resolved!
Implementation
LAS Reticule is an optional application that is combined with a Leica microscope, digital imaging camera and computer. Traditional methods of inserting a graticule disk etched with a square grid for meausurments (also known as an eyepiece micrometer) has many limitations which LAS Reticule strives to counteract. The electronic reticule is not only more cost-effective but also provides a more pleasurable working environment where the live microscope image is displayed on screen and an unlimited range of reticule styles can be overlayed. Additionally, reticules can be designed and applied quickly and easily, so the set can be extended as and when it’s necessary.
Application Features
Reticule information can be saved either by producing it in overlay format or by burning it into the image. When the reticule is merged into the image, it means that it is permanent. Additionally, the image can be re-opened using another application with the reticule information embedded.
The system also provides an option to save the reticule as an overlay that can later be removed or altered. A copy of the reticule file will be made and stored with the image, so if the master reticule is subsequently altered the stored image and reticule remains unaltered. When the reticule is stored as an overlay, the image can be opened in another application, but the reticule information will not be present.
Other advantages include:
• Reticules can be fixed or scaleable for additional flexibility.
• Standard user and power user modes allow varying responsibilities.
• An unlimited number of different reticules can be used.
• Additional reticules can be applied on a stored image.
Graphic Definition
Reticule Definition
A file in the graphic definition format “SVG” is used to define reticules. These can be of a fixed size so that they can be compared with the underlying image in a relative manner. Scalable reticules, of an absolute size, can be positioned (moved) and rotated easily and used in Live or Offline mode. The definition file may contain lines, shapes, and text, with user-defined properties. Even colour Images can be displayed in the reticule for additional versatility.
LAS is based on Windows PCs and provides a cost-effective and uniform environment, compatible across the Leica range of microscopes and cameras. Furthermore, images may be exported for additional processing.
Visualisation
Further application features are available to make the reticule user environment more intuitive:
• A Gallery of images is available from which individuals can be immediately selected.
• One of the available reticules may be selected and superimposed on the displayed stored image and scaled according to the calibration.
• Images and reticule definition can be saved to in a named folder so that they can be recalled with the attached reticule.
• Images can be exported for further documentation.
Monitor Image: Reticule of a gear wheel taken with a motorized Leica MZ16 A Stereomicroscope with a Leica Digital Camera
@
www.leica-microsystems.com
LAS Reticule
The Leica Application Suite Reticule module is primarily used to display customised reticules on live images. The electronic reticule is a replacement for the glass variants that have commonly been placed in the optical path of a microscope during routine manual inspection, and now offers greater versatility.
The Reticule module extends these capabilities by providing:
• A live image display on to which a reticule can be electronically overlayed to give visual feedback as to the approximate scale of the field of view.
The ability to carry out object size com­parisons
or point counting measurements.
• Greater flexibility due to the unlimited range of electronic reticule styles which can be automatically adjusted to the magnification of the microscope.
• A more comfortable working environment with on screen visualisation.
• The capacity to move and rotate a reticule even when viewing a live image.
• Easy reticule design and creation so each system can be extended in its scope.
To calibrate the Leica MZ16 A and MZ16 FA for use with the Reticule module, the following accessories are recommended:
Stage Micrometer 10310345 Graticule MZ16 A 10447185
and for additional convenience:
Gliding stage 10446301
Order number 12730065 Leica Application Suite Reticule Module
Leica Microsystems (Switzerland) Ltd Stereo & Macroscope Systems CH-9435 Heerbrugg
Telephone +41 71 726 33 33 Fax +41 71 726 33 99 www.leica-microsystems.com www.stereomicroscopy.com
Illustrations, descriptions and technical data are not binding and may be changed without notice.
Printed on chlorine-free paper with a high content of recycled fibre.
M1-525-3en • © Leica Microsystems (Switzerland) Ltd • CH-9435 Heerbrugg, 2005• Printed in Switzerland – VIII.2005 – RDV
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