Leica APPLICATION SUITE RETICULE, LAS RETICULE User Manual

Leica

Application

Suite

Reticule

Reticules Resolved!

Implementation

LAS Reticule is an optional application that
is combined with a Leica microscope, digital
imaging camera and computer. Traditional
methods of inserting a graticule disk etched
with a square grid for meausurments (also
known as an eyepiece micrometer) has
many limitations which LAS Reticule strives
to counteract. The electronic reticule is not
only more cost-effective but also provides
a more pleasurable working environment
where the live microscope image is
displayed on screen and an unlimited
range of reticule styles can be overlayed.
Additionally, reticules can be designed and
applied quickly and easily, so the set can be
extended as and when it’s necessary.

Application Features

Reticule information can be saved either by
producing it in overlay format or by burning
it into the image. When the reticule is
merged into the image, it means that it is
permanent. Additionally, the image can be
re-opened using another application with
the reticule information embedded.

The system also provides an option to save
the reticule as an overlay that can later be
removed or altered. A copy of the reticule
file will be made and stored with the image,
so if the master reticule is subsequently
altered the stored image and reticule remains
unaltered. When the reticule is stored as an
overlay, the image can be opened in another
application, but the reticule information will
not be present.

Other advantages include:

• Reticules can be fixed or scaleable for
additional flexibility.

• Standard user and power user modes
allow varying responsibilities.

• An unlimited number of different reticules
can be used.

• Additional reticules can be applied on a
stored image.

Graphic Definition

Reticule Definition

A file in the graphic definition format “SVG”
is used to define reticules. These can be of
a fixed size so that they can be compared
with the underlying image in a relative
manner. Scalable reticules, of an absolute
size, can be positioned (moved) and rotated
easily and used in Live or Offline mode. The
definition file may contain lines, shapes, and
text, with user-defined properties. Even
colour Images can be displayed in the
reticule for additional versatility.

LAS is based on Windows PCs and provides
a cost-effective and uniform environment,
compatible across the Leica range of
microscopes and cameras. Furthermore,
images may be exported for additional
processing.

Visualisation

Further application features are available to
make the reticule user environment more
intuitive:

• A Gallery of images is available from
which individuals can be immediately
selected.

• One of the available reticules may be
selected and superimposed on
the displayed stored image and scaled
according to the calibration.

• Images and reticule definition can be
saved to in a named folder so that they
can be recalled with the attached
reticule.

• Images can be exported for further
documentation.

Monitor Image: Reticule of a gear
wheel taken with a motorized
Leica MZ16 A Stereomicroscope
with a Leica Digital Camera

@

www.leica-microsystems.com

LAS Reticule

The Leica Application Suite Reticule module is primarily used to display customised reticules on live images. The
electronic reticule is a replacement for the glass variants that have commonly been placed in the optical path of a
microscope during routine manual inspection, and now offers greater versatility.

The Reticule module extends these capabilities
by providing:

• A live image display on to which a reticule
can be electronically overlayed to give visual
feedback as to the approximate scale of the
field of view.

•

The ability to carry out object size com­parisons

or point counting measurements.

• Greater flexibility due to the unlimited range
of electronic reticule styles which can be
automatically adjusted to the magnification
of the microscope.

• A more comfortable working environment
with on screen visualisation.

• The capacity to move and rotate a reticule
even when viewing a live image.

• Easy reticule design and creation so each
system can be extended in its scope.

To calibrate the Leica MZ16 A and MZ16 FA for
use with the Reticule module, the following
accessories are recommended:

Stage Micrometer 10310345
Graticule MZ16 A 10447185

and for additional convenience:

Gliding stage 10446301

Order number
12730065 Leica Application Suite Reticule Module

Leica Microsystems (Switzerland) Ltd
Stereo & Macroscope Systems
CH-9435 Heerbrugg

Telephone [phone]
Fax [phone]
www.leica-microsystems.com
www.stereomicroscopy.com

Illustrations, descriptions and technical data are not binding and may be changed without notice.

Printed on chlorine-free paper with a high content of recycled fibre.

M1-525-3en • © Leica Microsystems (Switzerland) Ltd • CH-9435 Heerbrugg, 2005• Printed in Switzerland – VIII.2005 – RDV