LAB-LINE 320, 320-1, 322, 322-1, 325 User manual

OPERATION

MANUAL

MANUAL NO. 056-761-00

REV. G

LAB-LINE

AIR-JACKETED

FLO-THRU CO2 INCUBATORS

MODEL NO. 320, 320-1

9/01

322, 322-1
325, 325-1

DESIGNERS AND MANUFACTURERS

A SUBSIDIARY of Barnstead|Thermolyne

1999 North 15th Ave.

PHONE: [phone] or [phone]; FAX: [phone]

, Melrose Park, IL 60160-1491 USA

TABLE OF

CONTENTS

SECTION TITLE

2

1 Introduction

2 Description

3 Specifications

4 Installation

5 Operation

6 Maintenance

7 Replacement Parts

Warranty

3

BE ADVISED:

IT IS MOST IMPORTANT THAT THE USER FOLLOW INSTALLATION
INSTRUCTIONS EXACTLY AS WRITTEN—FAILURE TO DO SO IS LIKELY TO
LEAD TO IMPROPER OPERATION, ERRONEOUS CALIBRATIONS AND
POSSIBLE DAMAGE TO THE EQUIPMENT. UNDER NO CIRCUMSTANCES
SHOULD THE USER ATTEMPT OPERATION WITHOUT THIS INFORMATION.

THE FOLLOWING EQUIPMENT IS TO BE SUPPLIED BY THE USER:

• Dry CO2 gas (research grade or better).

• A dual-stage regulator for the CO2 tank.

• ¼" (6.35mm) ID Flexible tubing (appropriate length from tank to

Incubator), and connected per local codes.

• Distilled or deionized water (if humidification is desired).

• Fyrite or similar chemical-based CO2 analyzer.

BE ADVISED: INSTALLATION AND PRE-OPERATION PROCEDURE WILL
TAKE AT LEAST 24 HOURS TO COMPLETE. DO NOT ATTEMPT TO RUSH
THE PROCESS WITH SHORT CUTS TO THE PROCEDURES DESCRIBED IN
THIS MANUAL.

CERTIFICATION OF DECONTAMINATION:

4

We cannot accept for service or credit a product that has been exposed
to or contaminated with chemically or biologically toxic or infectious substances
or subjected to radioactivity without first being certified as free from said
contamination.

Please have your Medical and/or Safety Officer sign this form certifying
that proper decontamination procedures have been followed to render the
product safe and free from hazards.

Any product forwarded to us, not accompanied by this form and a proper
Return Goods Authorization Number, will be returned to the sender. To obtain a
Return Goods Authorization Number, contact the Customer Relations
Department at [phone].

We hereby certify that the LAB-LINE INSTRUMENTS, INC. product:

Model No. and Serial No. ,

that is being forwarded has been properly decontaminated and is free from all toxic
hazards, infectious agents, radioactivity and/or other hazards.

Company/Institution Name:

Street Address:

City: State Zip

Name (please print): Title

Signature:

Phone:

DECONTAMINATION PROCEDURE (Be Specific):

Nature of Hazard That Required Decontamination:

5

SECTION 1

INTRODUCTION

6

THANK YOU

for selecting Lab-Line Instruments for your equipment needs. For maximum value and
ease of start-up,

PLEASE PROCEED AS FOLLOWS:

• Inspect the carton and contents for shipping damage. Notify the carrier immediately

if damage is found.

• Use the Accessory Checklist when unpacking to verify that the complete unit has

been received. Do not discard packing materials until all is accounted for.

• Read this Operation Manual thoroughly before deciding upon an appropriate location

for the unit: you will want to consider the availability of power and other unit
requirements, as well as user convenience.

• Insist that every operator of this unit becomes familiar with the Operation Section of

this manual.

• Be sure to fill out the Warranty Registration Card and mail it in to Lab-Line

Instruments within seven (7) days after receiving the unit.

IF

after reading this manual you should have any difficulties with the installation or
operation instructions, please call:

Lab-Line Customer Relations Department

[phone], or [phone]

ALL RIGHT RESERVED

The information contained in this manual is the exclusive property of Lab-Line Instruments, Inc., and has been pro­vided solely to enable the users of the equipment described herein to operate and maintain such equipment. Any
other use of this information, or the reproduction or transmission of all or any portion of this manual without prior
written consent of the manufacturer is expressly prohibited. © 2001, Lab-Line Instruments, Inc.

SECTION 2

DESCRIPTION

The Lab-Line Air-Jacketed Flo-Thru CO2 Incubator controls CO2
concentration and temperature to create a growth environment suitable for tissue
cultures, plants, cells, viruses and related organisms.

7

Incoming gas mixture passes through the rear plenum and is gently
circulated throughout the chamber. Adjustable flow meters control air and CO2
concentrations. An access port on the control panel permits chamber gas
samples to be taken.

A microprocessor-based controller maintains temperature while a
hydraulic thermostat provides over-temperature protection to the chamber in the
event the main control should fail.

Heaters outside the chamber inner walls are positioned for uniform heat
distribution, while high-density fiberglass insulation minimizes heat loss. A fan in
the chamber ceiling gently circulates chamber atmosphere to prevent
temperature and CO2 stratification.

A double-door system enables unobstructed viewing of the entire
chamber. In the steel outer door are heaters that can be turned on to defog the
glass inner door, if necessary. Heat control for the steel outer door is on the
back panel.

The leak proof chamber is constructed of heavy-gauge stainless steel and
the steel cabinet has a powder coated finish for easy cleaning. Stainless steel
shelves are adjustable and removable.

Differences between models in this series are the number of incubation
chambers (one or two), and power requirements.

MODEL 320:

POWER REQUIREMENTS:

320: 120 V 60 Hz 6.3 Amps 750 Watts

320-1: 230/240 V 50 Hz 3.1 Amps 750 Watts

322: 120 V 60 Hz 12.5 Amps 1500 Watts

322-1: 230/240 V 50 Hz 6.3 Amps 1500 Watts

325: 120 V 60 Hz 12.5 Amps 1500 Watts

325-1: 230/240 V 50 Hz 6.3 Amps 1500 Watts

SECTION 3

SPECIFICATIONS

8

TEMPERATURE

Range: From slightly above ambient to 60ºC

HUMIDITY RANGE: From ambient to 98% relative humidity.

CARBON DIOXIDE RANGE: From 0 to 20% of the mixture.

DIMENSIONS

320, 320-1 24-5/8"W x 25"D x 33"H 22"W x 22"D x 22"H

SINGLE: (63 x 64 x 84 cm) (56 x 56 x 56 cm)

322, 322-1 49¼"W x 25"D x 33"H 22"W x 22"D x 22"H

DUAL, HORIZONTAL: (125 x 63 x 84 cm) (56 x 56 x 56 cm)

325, 325-1 24-5/8"W x 25"D x 66"H 22"W x 22"D x 22"H

DUAL, VERTICAL: (63 x 64 x 168 cm) (56 x 56 x 56 cm)

VOLUME SHELVES* SHELF AREA

320, 320-1: 6.2 cu. ft. 5 13.4 sq. ft.
322, 322-1: 12.4 cu. ft. 10 26.8 sq. ft.
325, 325-1: 12.4 cu. ft. 10 26.8 sq. ft.

*A MAXIMUM OF 10 SHELVES IN SINGLE UNIT AND 20 SHELVES
IN DUAL UNITS CAN BE USED.

OVERALL CHAMBER

UNIT’S ENVIRONMENTAL OPERATING CONDITIONS:

POLLUTION DEGREE: 2

INSTALLATION CATEGORY: II

ALTITUDE: 2000 Meters MSL (Mean Sea Level)
HUMIDITY: 80% maximum, non-condensing

ELECTRICAL SUPPLY: 120VAC or 240VAC

VOLTAGE TOLERANCE: ±10% of normal rated line

TEMPERATURE: 15ºC to 40ºC

PRODUCT USAGE: This product is intended for use indoors only

√SHIPPING CARTON:

This should be inspected upon delivery. When received, carefully
examine for any shipping damage before unpacking. If damage is discovered,
the delivering carrier should both specify and sign for the damage on your copy
of the delivery receipt.

Open the carton carefully making certain that all parts are accounted for
before packaging materials are discarded—after unpacking, if damage is found,
promptly report it to the carrier and request a damage inspection promptly.

SECTION 4

INSTALLATION

9

IMPORTANT: Failure to request an inspection of damage within a few
days after receipt of shipment absolves the carrier from any liability for damage:
you must call for a damage inspection promptly.

DANGER: DO NOT USE IN THE PRESENCE OF FLAMMABLE OR
COMBUSTIBLE MATERIALS OR EXPLOSIVE GASES. DO NOT USE IN THE
PRESENCE OF PRESSURIZED OR SEALED CONTAINERS—FIRE OR
EXPLOSION MAY RESULT, CAUSING DEATH OR SEVERE INJURY.

WARNING: DO NOT HEAT ANY SUBSTANCE ABOVE A TEMPERATURE
THAT WILL CAUSE IT TO EMIT TOXIC FUMES—DEATH OR SEVERE
INJURY MAY RESULT.

CAUTION: THIS UNIT IS DESIGNED FOR USE WITH GASEOUS AIR, CO2 OR
NITROGEN ONLY. DO NOT USE WITH ANY OTHER GASES. FAILURE TO
OBSERVE THESE PRECAUTIONS CAN RESULT IN EXPLOSION AND/OR
FIRE AND SERIOUS INJURY AND/OR DEATH AND PROPERTY DAMAGE.

LOCATION:

Place the unit where it will be used, away from external vibration sources,
drafts and wide variations in ambient temperature. Choose a location near a
power supply that matches the unit nameplate requirements.

ELECTRICAL CONNECTION:

120 VAC models require a 120 VAC, 50/60 Hz power source. They are
supplied with a 3-wire line cord. It should be plugged into an outlet designed for
3-prong plugs. If an extension cord is used, it also should be the 3-wire grounded
type. For an outlet designed to accept 2-prong plugs (ungrounded), it is required
that a qualified electrician replaces the outlet with a new, grounded type.

240 VAC models require a 240 VAC, 50/60 Hz power source. Because of
the variety of plug configurations in use worldwide for 240 VAC power, the unit is
furnished with the plug removed. The user must install a plug to conform to local
code and configuration requirements.

If a plug must be installed, use only the 3-prong grounded type, rated for
the unit load requirements and matching the power outlet. Make sure the green
ground wire is secured to the plug ground terminal.

NOTE: LEAVE UNIT DISCONNECTED WHEN NOT IN USE.

INSTALLATION: (Con’t)

HUMIDIFICATION:

To raise the humidity level in the chamber above ambient, add water to the

humidity pan in the chamber.

AIR/CO2 HOOK-UPS:

Turn the power switch to OFF and insert the plug into the outlet.

TO AIR FITTING 1/4” HOSE BARB

10

CONNECTIONS

TO CO2 FITTING

SET PRESSURE AT 10 PSI.

PRESSURE REGULATORS AND GAUGES MUST
BE INSTALLED ON EACH TANK. CHECK LOCAL
GAS SUPPLIER FOR PROPER REGULATORS.

IF AIR PUMP IS USED INSTEAD OF TANK,
AIR MUST BE FILTERED TO REMOVE WATER,
OIL AND DIRT.

100% AIR 100% CO2

Turn all flow meters on the incubator fully clockwise (closed). Connect air
and CO2 supply lines to their respective fittings on the back of the unit. Use 1/4"
ID tubing, flexible metal hose, etc., (meeting local codes), with filters to insure
that the air and CO2 are free of contaminants, for gas lines.

Set the supply regulators at 10 psi. Check all of the connections for leaks.

NOTE: FOR DUAL CHAMBER MODELS, EACH CHAMBER HAS A SEPARATE SET OF AIR
AND GAS FITTINGS.

WARNING: HIGH CONCENTRATIONS OF CARBON DIOXIDE PRODUCE
METABOLIC ABNORMALITIES, DISTURBANCES OF THE CENTRAL
NERVOUS SYSTEM AND CARDIAC INSTABILITY. UNCONSCIOUSNESS
MAY OCCUR AT CONCENTRATIONS ABOVE 10%.

INSTALLATION: (Con’t)

SHELVING/PLENUM INSTALLATION-SELF-SUPPORTING SYSTEM COMPONENTS:

TOP PLENUM SHELF SUPPORT SIDE PANELS

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REAR COVER PANEL

SHELVING/PLENUM INSTALLATION-SELF-SUPPORTING SYSTEM ASSEMBLED

TOP PLENUM

SHELF SUPPORT SHELF SUPPORT

SIDE PANEL SIDE PANEL

REAR COVER PANEL

INSTALLATION: (Con’t)

SHELVING AND PLENUM INSTALLATION-4 STEPS:

NOTE: NO TOOLS OR FASTENERS ARE REQUIRED. THE COMPONENTS FIT
TOGETHER INTO A SELF-SUPPORTING ASSEMBLY.

TOP PLENUM, HEAD-ON VIEW:

KEYWAY STUDS

(ONTO WHICH THE CHAMBER’S REAR
COVER PANEL KEYHOLE SLOTS WILL FIT)

12