Lab-Line 314, 314-1, 314-1CE, 316, 316-1 User manual

1/01

OPERATION MANUAL

MANUAL NO. 057-561-00

REV. A

LAB-LINE®

AIR-JACKETED COMPACT

AUTOMATIC & FLO-THRU®

CO2 INCUBATORS

MODEL NO. AUTOMATIC:

314, 314-1, 314-1CE

(Stainless Steel Interiors)
316, 316-1

(Copper Interiors)
FLO-THRU:

315, 315-1
(Stainless Steel Interiors)

317, 317-1
Copper Interiors)

1999 North 15th Ave., Melrose Park, IL 60160-1491 USA

PHONE: [phone] or [phone]; FAX: [phone]

DESIGNERS AND MANUFACTURERS

A SUBSIDIARY of Barnstead|Thermolyne

TABLE OF CONTENTS

2

SECTION TITLE

1 Introduction
2 Description
3 Specifications
4 Features
5 Installation
6 Operation
7 Maintenance
8 Replacement Parts
Warranty

3

BE ADVISED:

IT IS MOST IMPORTANT THAT THE USER FOLLOW INSTALLATION
INSTRUCTIONS EXACTLY AS WRITTEN—FAILURE TO DO SO IS LIKELY TO
LEAD TO IMPROPER OPERATION, ERRONEOUS CALIBRATIONS AND
POSSIBLE DAMAGE TO THE EQUIPMENT. UNDER NO CIRCUMSTANCES
SHOULD THE USER ATTEMPT OPERATION WITHOUT THIS INFORMATION.

THE FOLLOWING EQUIPMENT IS TO BE SUPPLIED BY THE USER:

• Dry CO

• A dual-stage regulator for the CO2 tank.

• ¼" ID Flexible tubing (appropriate length from tank to incubators), and

connected per local codes.

• Fyrite or similar chemical-based CO2 analyzer.

BE ADVISED: INSTALLATION AND PRE-OPERATION PROCEDURE WILL
TAKE AT LEAST 24 HOURS TO COMPLETE. DO NOT ATTEMPT TO RUSH
THE PROCESS WITH SHORT CUTS TO THE PROCEDURES DESCRIBED IN
THIS MANUAL.

2 gas (research grade or better).

4

CERTIFICATION OF DECONTAMINATION:

We cannot accept for service or credit a product that has been exposed

to or contaminated with chemically or biologically toxic or infectious substances
or subjected to radioactivity without first being certified as free from said
contamination.
Please have your Medical and/or Safety Officer sign this form certifying
that proper decontamination procedures have been followed to render the
product safe and free from hazards.
Any product forwarded to us, not accompanied by this form and a proper

Return Goods Authorization Number, will be returned to the sender. To obtain a
Return Goods Authorization Number, contact the Customer Relations
Department at [phone].

We hereby certify that the LAB-LINE INSTRUMENTS, INC. product:

Model No. and Serial No. ,
which is being forwarded has been properly decontaminated and is free from all toxic

hazards, infectious agents, radioactivity and/or other hazards.
Company/Institution Name:
Street Address:
City: State Zip
Name (please print): Title
Signature:
Phone:
DECONTAMINATION PROCEDURE (Be Specific):

Nature of Hazard That Required Decontamination:

5

6

SECTION 1

INTRODUCTION

THANK YOU

for selecting Lab-Line Instruments for your equipment needs. For maximum value and
ease of start-up,

PLEASE PROCEED AS FOLLOWS:

• Inspect the carton and contents for shipping damage. Notify the carrier immediately
if damage is found.

• Use the Accessory Checklist when unpacking to verify that the complete unit has
been received. Do not discard packing materials until all is accounted for.

• Read this Operation Manual thoroughly before deciding upon an appropriate location
for the unit: you will want to consider the availability of power and other unit
requirements, as well as user convenience.

• Insist that every operator of this unit becomes familiar with the Operation Section of
this manual.

• Be sure to fill out the Warranty Registration Card and mail it in to Lab-Line
Instruments within seven (7) days after receiving the unit.

IF

after reading this manual you should have any difficulties with the installation or
operation instructions, please call:

Lab-Line Customer Relations Department
[phone] or [phone]

ALL RIGHT RESERVED

The information contained in this manual is the exclusive property of Lab-Line Instruments, Inc., and has been pro­vided solely to enable the users of the equipment described herein to operate and maintain such equipment. Any
other use of this information, or the reproduction or transmission of all or any portion of this manual without prior
written consent of the manufacturer is expressly prohibited. © 2001, Lab-Line Instruments, Inc.

SECTION 2

7

DESCRIPTION

314/316 SERIES AUTOMATIC CO2 INCUBATORS:

BASIC COMPONENTS, AN INITIAL OVERVIEW:

1 13

14

2
3
4
5
6

7

8
15 9 10 11 12

1. Control panel

2. CO2 sensor

3. CO

4. Temperature sensor

5. Chamber gasket

6. Chamber shelves

7. Sample port inlet

8. Leveling feet

inlet port

2

9. Inner glass door, handle shaft

10. Inner glass door

11. Inner glass door, handle

12. Outer door gasket

13. CO

14. Fan Cover

15. Humidity Trough

sample port

2

DESCRIPTION: (Con’t)

8

314/316 SERIES AUTOMATIC CO2 INCUBATORS: (Con’t)

BASIC COMPONENTS, AN INITIAL OVERVIEW: (CON’T)
CHAMBER CEILING, INTERIOR

CIRCULATING FAN

REAR PANEL VIEW, EXTERIOR:
1 4
2 5
3 6

1. GAS INLET PORT

2. CORDSET

3. CIRCUIT BREAKERS

4. RS232 DATA PINS CONNECTOR

5. POWER SWITCH

6. OPTIONAL ALARM RELAY OUTPUT

DESCRIPTION: (Con’t)

314/316 SERIES AUTOMATIC CO2 INCUBATORS:

9

CO2 levels are automatically set at 5%. These units control CO2
concentrations, as well as temperature, to create a growth environment ideal for
tissue cultures, plants, cells, viruses and related organisms.
Incoming 100%, CO2, from an external source provided by the user,
passes into the gas inlet port located on the rear panel of the unit. Heated air is
moved uniformly throughout the chamber by a circulating fan located in the top of
the chamber. A CO2 access port on the control panel permits sampling of the
chamber atmosphere.
These incubators provide precise and reliable control of carbon dioxide
through the use of a TC, thermal conductivity, sensor coupled with an easy-to­use command control console. All required operating commands are
immediately accessible in a functional format on the front of the unit and permit

rapid start-up, close monitoring and efficient control of incubation protocols.
Temperature is measured by a platinum RTD, resistance temperature
detector, producing excellent control within the range, ±0.1ºC. This is combined
with the exclusive SmartGas® algorithm method for controlling CO2 within the
range ±0.1%. The result is a chamber that produces excellent environmental
conditions for the most sensitive of incubations.
Audible and visual alarms provide a signal to the user of any deviation in

the chamber temperature of ±1.0ºC, or carbon dioxide of ±1.0% CO2. In

addition, other signals alert the user to power failures and door openings.
An independent, adjustable hydraulic high limit thermostat will limit the
temperature in the event of a problem with the main temperature controller.
To conserve gas, the unit’s CO2 solenoid valve, as well as the circulating
fan motor, shuts off automatically any time the door is opened.
A double-door system has a tempered glass inner door that provides
unobstructed viewing of the entire chamber.

314 series chambers are constructed of seamless stainless steel for

cleaning ease and maintenance; 2 stainless steel shelves are standard—a total

capacity is 5 shelves. 316 series incubators feature a fungus and bacteria

resistant copper chamber.
Both models have a stainless steel outer cabinet with a powder coated
finish for easy cleaning.

DOUBLE-DOOR CONSTRUCTION WITH INNER DOOR DEFOGGER: 314/316
MODELS:

A tempered glass inner door allows observation of a chamber without
disturbing the chamber environment. Inside the steel outer door are heaters
that can be turned on to defog the glass door when necessary. Each door
seals to a one-piece gasket for a leak-tight environment.

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DESCRIPTION: (Con’t)

315/317 SERIES FLO-THRU CO2 INCUBATORS:

BASIC COMPONENTS, AN INITIAL OVERVIEW:

1

2 3 4 5 6 7 8 9 10

1. AIR & CO2

FLOWMETERS

2. UNIT FEET

3. CO2 ACCESS PORT

4. CHAMBER SHELVES

5. TEMPERATURE SENSOR

6. CHAMBER GASKET

7. INNER GLASS DOOR, HANDLE SHAFT

8. INNER GLASS DOOR

9. INNER GLASS DOOR, HANDLE

10. OUTER STEEL DOOR/GASKET

11

DESCRIPTION: (Con’t)

315/317 SERIES FLO-THRU CO2 INCUBATORS: (Con’t)

BASIC COMPONENTS, AN INITIAL OVERVIEW: (CON’T)

CHAMBER CEILING, INTERIOR

CIRCULATING FAN

REAR PANEL VIEW, EXTERIOR:

CO2

1 2

1. CO2 INLET PORT

2. CORDSET

12

DESCRIPTION: (Con’t)

315/317 SERIES FLO-THRU CO2 INCUBATORS:

CO2 levels are manually adjusted utilizing flowmeters. Flo-Thru control of

CO2 concentrations and temperature create a growth environment ideal for tissue

cultures, plants, cells, viruses and related organisms.
An incoming gas mixture passes through the plenum and is gently
circulated throughout the chamber. Separate flowmeters adjust air and CO2
rates and an access port on the control panel permits sampling of the chamber
atmosphere.
2 hydraulic thermostats: one is an adjustable setpoint control unit, the
other is a non-adjustable, over-temperature-limiting unit. It is factory pre-set to
regulate temperature in order to protect the chamber.
Heaters on the outside of the chamber inner walls are positioned for
uniform heat distribution, while high-density fiberglass insulation minimizes heat
loss.
A double-door system has a tempered glass inner door that provides
unobstructed viewing of the entire chamber.

Model 315 and 315-1 interiors are constructed of seamless stainless steel

for cleaning ease and maintenance. 2 stainless steel shelves are standard—a

total capacity is 5 shelves.
Models 317 and 317-1 have a seamless copper interior that acts to reduce

the growth of fungus and bacteria thereby minimizing contamination of
cultures. 2 copper shelves are standard with room for 3 additional shelves.
All models have a steel outer cabinet with a powder coated finish for easy
cleaning.

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SECTION 3

SPECIFICATIONS

314/316 SERIES AUTOMATIC CO

ELECTRICAL REQUIREMENTS:
314, 316: 120 V, 60 Hz, 200 Watts, 1.7 Amps,
314-1, 316-1: 230/240 V, 50 Hz, 200 Watts, 0.8 Amps,

TEMPERATURE:
RANGE: Ambient +5ºC to 60ºC
CONTROL: ±0.1ºC at 37ºC.
UNIFORMITY IN CHAMBER: Within the chamber, ±0.25ºC

ALGORITHM: TEMPERATURE: PID
CO2: SmartGas®

CARBON DIOXIDE TENSION: Range: 0% to 20%
Control: ±0.1%

VOLUME: 1.2 cubic feet (34 liters)
EXTERIOR: 22½" W x 18" D x 19" H

(57 x 46 x 48 cm)
INTERIOR: 14" W x 12" D x 12" H

(36 x 31 x 31 cm)
SHELVES: 2 standard; space for 3 additional shelves.

DIMENSIONS: 13-3/8” (tapering back to) 12-1/8" W x 11½" D
(34~30.7 x 29.2 cm)

SHIPPING WEIGHT: 70 lbs. (32 kg)

2 INCUBATORS:

SPECIFICATIONS: (Con’t)

14

315/317 SERIES FLO-THRU CO2 INCUBATORS:

ELECTRICAL REQUIREMENTS:
315, 317: 120 V, 60 Hz, 200 Watts, 1.7 Amps,
315-1, 317-1: 230/240 V, 50 Hz, 200 Watts, 0.8 Amps,

TEMPERATURE:
RANGE: Slightly above ambient to 60ºC.
CONTROL: ±0.5ºC.
UNIFORMITY: ±1.0ºC
CARBON DIOXIDE RANGE: From 0 to 20% of the mixture.

VOLUME: 1.2 cubic feet (34 liters)
DIMENSIONS:

EXTERIOR: 23½" W x 17" D x 15" H
(60 x 43 x 38 cm)
INTERIOR: 14" W x 12" D x 12" H
(36 x 31 x 31 cm)

SHELVES: 2 standard; space for 3 additional shelves.
DIMENSIONS: 11"W x 13½"D x 7"H (28 x 34 x 18 cm)

SHIPPING WEIGHT: 70 lbs. (32 kg)

UNIT’S ENVIRONMENTAL OPERATING CONDITIONS:

POLLUTION DEGREE: 2
INSTALLATION CATEGORY: II
ALTITUDE: 2000 Meters MSL (Mean Sea Level)
HUMIDITY: 80% maximum, non-condensing
ELECTRICAL SUPPLY: 120VAC or 240VAC
VOLTAGE TOLERANCE: ±10% of normal rated line
TEMPERATURE: 15ºC to 40ºC
PRODUCT USAGE: This product is intended for use indoors only

SECTION 4

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