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Congratulations on your purchase of a Labconco
CentriVap DNA Concentrator System. Models are
available for operation on 115V or 230V.
The CentriVap Concentrator uses centrifugal force with
heat and vacuum to rapidly dry precipitates of DNA
and RNA samples. Centrifugation eliminates bumping
and foaming as vacuum is applied and also concentrates
the solute in the bottom of the vial. This allows
recovery of solutes from sample volumes as small as a
few microliters. The Concentrator is equipped with a
300 watt heater to speed evaporation by warming the
chamber during processing. A timed outlet for the
vacuum pump connection delays the start of the pump
until the rotor reaches operating speed. A safety switch
prevents the concentrator from starting when the lid is
in the open position and a latch prevents the lid from
being opened while the rotor is turning. The compact,
low profile design allows the Concentrator to be placed
on a bench top or other laboratory work surface.
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All wetted parts within the pump are either Teflon*,
Teflon coated or stainless steel. A glass trap is
provided to remove liquid from the system before it
enters the pump and a second glass trap is provided on
the outlet side of the pump to trap liquid expelled from
the pump.
*Teflon® is a registered trademark of DuPont
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Chapter 1: Introduction
The microprocessor which controls the operation of the
heater and the motor provides excellent regulation and
reproducibility of protocols. The end of the run can be
signaled by a manually preset timer. Nine different
protocols can be stored in memory so exact test
parameters can be easily repeated.
About This Manual
This manual is designed to help you learn how to
install, use, and maintain your CentriVap. Instructions
for performing routine maintenance and making minor
modifications to your CentriVap are also included.
Chapter 1: Introduction provides a brief overview of
the CentriVap, explains the organization of the manual,
and defines the typographical conventions used in the
manual.
Chapter 2: Prerequisites explains what you need to do
to prepare your site before you install your CentriVap.
Electrical and exhaust requirements are discussed.
Chapter 3: Getting Started contains the information
you need to properly unpack, inspect and install your
CentriVap.
Chapter 4: Using Your CentriVap discusses the basic
operation of your CentriVap. Information on how to
arrange the samples inside your CentriVap and select an
operating cycle is included.
Chapter 5: Maintaining Your CentriVap explains how
to perform routine maintenance on your CentriVap.
Chapter 6: Modifying Your CentriVap describes how to
install the optional glass lid.
Chapter 7: Troubleshooting contains a table of
problems you may encounter while using your
CentriVap, including the probable causes of the
problems, and suggested corrective actions.
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Appendix A: CentriVap Components contains labeled
diagrams of the components of the CentriVap.
Appendix B: CentriVap Dimensions contains
comprehensive diagrams showing the dimensions for
the CentriVap.
Appendix C: CentriVap Specifications contains product
specifications. Wiring diagrams for both the 115V and
230V CentriVap are also included.
Appendix D: CentriVap Accessories lists the part
numbers and descriptions of all of the accessories
available for your CentriVap.
Typographical Conventions
Chapter 1: Introduction
Recognizing the following typographical conventions
will help you understand and use this manual:
• Book, chapter, and section titles are shown in italic
type (e.g., Chapter 3: Getting Started).
• Steps required to perform a task are presented in a
numbered format.
• Comments located in the margins provide
suggestions, reminders, and references.
• Critical information is presented in boldface type in
paragraphs that are preceded by the exclamation
icon. Failure to comply with the information
following an exclamation icon may result in injury
to the user or permanent damage to your CentriVap.
• Important information is presented in capitalized
type in paragraphs that are preceded by the pointer
icon. It is imperative that the information contained
in these paragraphs be thoroughly read and
understood by the user.
!
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Chapter 1: Introduction
Your Next Step
If your CentriVap needs to be installed, proceed to
Chapter 2: Prerequisites to ensure your installation site
meets all of the requirements. Then, go to Chapter 3:
Getting Started for instructions on how to install your
CentriVap and make all of the necessary connections.
For information on the operational characteristics of
your CentriVap, go to Chapter 4: Using Your
CentriVap.
If your CentriVap is installed and you need to perform
routine maintenance on the CentriVap, proceed to
Chapter 5: Maintaining Your CentriVap.
For information on making modifications to the
configuration of your CentriVap go to Chapter 6:
Modifying Your CentriVap.
Refer to Chapter 7: Troubleshooting if you are
experiencing problems with your CentriVap.
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Before you install your CentriVap, you need to prepare
your site for installation. Carefully examine the
location where you intend to install your CentriVap.
You must be certain that the area is level and of solid
construction. In addition, an exhaust means must be
provided. An electrical source must be located near the
installation site.
Carefully read this chapter to learn:
• the electrical supply requirements.
• the exhaust requirements.
Refer to Appendix C: CentriVap Specifications for
complete CentriVap electrical and environmental
conditions, specifications and requirements.
The CentriVap has not been evaluated
by an approval agency for the use of
!!!!
biological, radiotoxins or flammable
liquids or materials.
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Electrical Requirements
A dedicated grounded electrical outlet is required. This
outlet requires a 15 Amp circuit breaker or fuse for
models rated at 115V (60 Hz). An 8 Amp circuit
breaker or fuse is required for models rated at 230V
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Chapter 2: Prerequisites
(50/60 Hz). 115V models are equipped with a 15 Amp
NEMA 5-15P plug. 230V models are not equipped
with a plug. It will be necessary to install a plug to
match the available receptacle.
It is recommended that an emergency switch for
disconnecting the mains in the case of a malfunction be
located remote from the CentriVap, preferably outside
the room in which the CentriVap is housed, or adjacent
to the exit from that room.
Location and Exhaust Requirements
The CentriVap DNA System should be located within a
fume hood if hazardous or flammable solvents are used.
In all cases, regardless of the solvent used, it is strongly
recommended that the vacuum pump is vented in a
fume hood. An optional accessory secondary trap is
available to minimize the exhausting of solvents into
the atmosphere. This does not, however, negate the
need to exhaust the vacuum pump into a fume hood.
Space Requirements
Refer to Appendix C: CentriVap Specifications for
dimensional drawings of the CentriVap.
Your Next Step
After you have determined that the location for your
CentriVap accommodates the installation and
operational requirements, you are ready to unpack and
install your CentriVap. Proceed to Chapter 3: Getting
Started.
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Now that the site for your CentriVap is properly
prepared, you are ready to unpack, inspect, install, and
test your CentriVap. Read this chapter to learn how to:
• unpack and move your CentriVap.
• set up your CentriVap.
• connect the electrical supply source to your
CentriVap.
• properly exhaust your CentriVap.
• safely use solvents with your CentriVap.
The CentriVap weighs over 69 lbs.
(31 Kg). The carton allows for lifting
!!!!
with a mechanical lift truck or hand
truck. If you must lift the CentriVap
manually, use at least two (2) persons
and follow safe lifting guidelines.
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Chapter 3: Getting Started
Unpacking Your CentriVap
The United States
Interstate Commerce
Commission rules
require that claims be
filed with the delivery
carrier within fifteen (15)
days of delivery.
Carefully unpack your CentriVap and inspect it for
damage that may have occurred in transit. If your
CentriVap is damaged, notify the delivery carrier
immediately and retain the entire shipment intact for
inspection by the carrier.
DO NOT RETURN GOODS
☞
☞
WITHOUT THE PRIOR
AUTHORIZATION OF LABCONCO.
UNAUTHORIZED RETURNS WILL
NOT BE ACCEPTED.
IF YOUR CENTRIVAP WAS
DAMAGED IN TRANSIT, YOU
MUST FILE A CLAIM DIRECTLY
WITH THE FREIGHT CARRIER.
LABCONCO CORPORATION AND
ITS DEALERS ARE NOT
RESPONSIBLE FOR SHIPPING
DAMAGE.
Do not discard the carton or packing material for your
CentriVap until you have checked all of the
components and installed and tested the CentriVap.
CentriVap Components
As previously mentioned, the CentriVap DNA System
is available in 115V or 230V. Locate the model of
CentriVap you received in the following table. Verify
that the components listed are present and undamaged.
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Catalog # Product Description
79700-00 DNA Concentrator – 115V
79700-01 DNA Concentrator – 230V
Plus the Following:
Part # Component Description
74572-00 User’s Manual
13345-00
13341-00
74629-00 Rotor
If you do not receive one or more of the components
listed for your CentriVap, or if any of the components
are damaged, contact Labconco Corporation
immediately for further instructions.
Power Cord – 115V
or
Power Cord – 230V
Setting Up Your CentriVap
Chapter 3: Getting Started
After you verify receipt of the proper components,
move your CentriVap to the level and sturdy location
where you want to install it. There is no need to secure
the CentriVap DNA System to the work surface. Then,
follow the steps listed below.
Emergency Access Into the
Chamber
The CentriVap is designed to prevent access to the
chamber in the event of a power disruption. If it is
necessary to open the lid when there is no electrical
power connected to the CentriVap, insert a small
screwdriver or similar instrument into the small round
hole on the left side of the case near the top behind the
control panel. This will unlock the lid latch
mechanism. While holding the screwdriver in place,
raise the lid with the other hand.
Never attempt to defeat the latch or
open the lid while the CentriVap is
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running.
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Chapter 3: Getting Started
Exhaust Port
If the CentriVap is not located in a fume hood, attach
one end of a .50 inch inside diameter hose to the
exhaust port on the left side on the vacuum pump glass
trap exhaust port. Clamp securely. Route the other end
to a fume hood or other laboratory ventilation device.
Electrical Connection
Plug the power cord into the receptacle on the back of
the CentriVap and plug the other end into a suitable
power receptacle.
Rotor Installation
Place the rotor onto the shaft of the Concentrator.
Rotate the rotor slightly to engage the drive pin in the
shaft with the slots in the rotor hub. The top of the
shaft should be in line with the top of the rotor hub.
!
!
Do not use a rotor if it shows any signs
of damage.
Solvent Safety Precautions
The CentriVap is not classified as
“explosion proof.” It has been
designed with safety as a primary
consideration
prudent manner using “good
laboratory practices.” It has been
designed for use with compounds as
described in the United States
National Electrical Code Class I,
Group D. The heater may be
programmed to run as hot as 100°C,
however, the heater element may
normally run at 110°C. A thermal
fuse limits the heater to a maximum
temperature of 141°C. It is important
and should be used in a
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that the solvents used are compatible
with these temperatures. Do not
evaporate solvents that have an
autoignition temperature below
180°C. Do not evaporate solvents that
are classified as Group A, B, or C by
the National Electrical Code.
Evaporate only non-flammable or
Group D solvents with autoignition
temperatures 180°C or above. Use of
other compounds could cause an
explosion.
Solvents used in the CentriVap may
be flammable or hazardous. Use
extreme caution and keep sources of
ignition away from the solvents.
When using flammable or hazardous
solvents, both the CentriVap and the
vacuum pump should be operated
inside a fume hood.
Chapter 3: Getting Started
If a sample is spilled in the chamber it
must immediately be cleaned up.
Hazardous materials such as strong
acids or bases, radioactive substances
and volatile organics, must be handled
carefully and promptly cleaned up if
spilled.
Do not store flammable or hazardous
solvents within 12 inches (300 mm) of
the CentriVap.
Several components of the CentriVap
which are located inside the chamber
are made of stainless steel which can
be attacked by acids. Use of acids
such as trifluoroacetic acid can result
in degradation of the product and the
vacuum pump if the vacuum system is
being used. Use care when using
aggressive liquids which can damage
the CentriVap and thoroughly clean
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Chapter 3: Getting Started
the CentriVap after each use. Contact
Labconco before evaporating acids.
WARNING: The disposal of
substances used in connection with
this equipment may be governed by
various Federal, State or local
regulations. All users of this
equipment are urged to become
familiar with any regulations that
apply in the user’s area concerning
the dumping of waste materials in or
upon water, land or air and to comply
with such regulations.
Your Next Step
The installation and setup of your CentriVap is now
complete. To learn how to load and operate your
CentriVap, proceed to Chapter 4: Using Your
CentriVap. To make a modification to the
configuration of your CentriVap, proceed to Chapter 6:
Modifying your CentriVap. To perform additional
diagnostics on your CentriVap, proceed to Chapter
7:Troubleshooting. To learn about the maintenance
requirements for your CentriVap, proceed to Chapter 5:
Maintaining Your CentriVap.
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After your CentriVap has been installed as detailed in
Chapter 3: Getting Started, you are ready to begin
using your CentriVap. Read this chapter to learn how
to:
• set operating parameters.
• operate the controls.
• properly select and position glassware inside
• understand the display.
• interrupt a cycle after it has begun.
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your CentriVap.
Do not use the CentriVap in a manner
not specified by the manufacturer
(refer to Appendix C: CentriVap
Specifications). The electrical
protection properties of the CentriVap
may be impaired if the CentriVap is
used inappropriately.
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Do not store or stack supplies or
equipment on top of the CentriVap.
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Chapter 4: Using Your CentriVap
Planning
Thoroughly understand procedures and the equipment
operation prior to beginning work. The unique
performance of the CentriVap is dependent upon the
proper balance of heat, vacuum and centrifugal force.
If the proper balance is not established, it is possible to
damage or lose a portion of the sample. Therefore, if
you are unfamiliar with the CentriVap or are attempting
a new protocol, it may be helpful to make a trial run
that is void of the sample you are attempting to
concentrate.
Glassware Selection
Normally, sample tubes should be filled no more than
approximately half full. Select the size of the sample
tube so it is compatible with the rotor and the desired
sample size. Tubes should not be loose in the rotor.
Rotors are available with holes for various size tubes.
Refer to Appendix D: CentriVap Accessories for
available rotor sizes and pages 19 and 20 for
recommended usable tube volumes.
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Loading Glassware Into the CentriVap
Smooth operation of the CentriVap is dependent upon
proper balance of the machine. Therefore, if less than a
full load of samples is run, it is important to load
samples into the CentriVap in a fairly symmetrical
manner distributing the weight of the samples evenly in
the sample rotor.
Heat Setting Guidelines
The evaporation rate achieved by the CentriVap is
dependent upon a variety of factors. These include the
nature of the solvent, the temperature and the pressure
in the vacuum system.
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Chapter 4: Using Your CentriVap
As a general guideline, to speed the evaporation
process, the CentriVap temperature should be set as
high as possible as long as the temperature will not
damage the sample or cause the sample to bump.
Time Setting Guidelines
The CentriVap Concentrator has two timers. The
“RUN TIME” turns the entire concentrator off after the
user set period of time. This stops the rotation, turns
off the heater and vacuum pump and bleeds vacuum
from the system. The heater may be turned off prior to
the concentrator turning off so heat sensitive samples
may be protected from exposure to excessive heat after
the solvent has evaporated. To turn the heater off prior
to stopping the entire system, enter a set time into the
memory at the “HEATER TIME”. When the
programmed time expires, the heater will turn off but
the rotation and vacuum will continue until the run time
expires.
The tables in Appendix C: CentriVap Specifications
indicate approximate times required to evaporate
various common solvents. Actual times must be
determined by the user. The CentriVap can be set to
alarm after a preset period of operation. When the time
expires, the CentriVap will give an audible alarm and
turn itself off.
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Chapter 4: Using Your CentriVap
4
CentriVap Controls
The control panel for the CentriVap is shown below
with a description about its function.
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1. Display – The liquid crystal display (LCD) shows
set point parameters and actual measured
conditions.
2. Program Buttons – Used to initiate the start of a run
with the use of just one button.
3. Run/Stop Button – Used to start or stop a run.
4. Preheat Button – Used to turn on the heater to
preheat the chamber prior to loading samples.
5. Increase Button – When pressed, the last selected
set point will increase.
6. Decrease Button – When pressed, the last selected
set point will decrease.
7. Set Point Select Button – To select a parameter to
change, press the select button. Arrows on the
display will point to the parameter that may be
altered.
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Operating The CentriVap
Controls
Preheat:
To preheat the chamber, press the “PREHEAT” button.
The display will show:
SP: XXX ACT: XXX
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Chapter 4: Using Your CentriVap
Press the increase or decrease button until the desired
set point (SP) is displayed. The actual chamber
temperature, (ACT) is displayed to the right. When
power is being supplied to the heaters, a bar under the
actual temperature will illuminate. The chamber will
continue to preheat until either the “PREHEAT” button
is pressed again or the “RUN” button is pressed.
Select existing program:
Operating parameters can be stored in memory so
protocols can be repeated. Nine programs can be
stored. To select a program, press the set point
“SELECT” button until arrows point to the program
number indicating that this set point can be run or
altered. To change the program number, press the
increase or decrease button until the desired program
number is displayed. When the program number is
changed, all its set points change also to indicate the
last entered parameters for that program.
Store frequently run protocols in program 1, 2, or 3.
Then, by pressing “PROG 1”, “PROG 2” or “PROG 3”,
the stored program will be initiated without having to
press any other button. Pressing just the one button
starts the rotor, the heater, the timers and the vacuum
pump.
Change heat set point:
To change the heat set point, press the set point
“SELECT” button until arrows point to the temperature
set point which can be changed from -- (OFF) up to 99°
by pressing the increase or decrease buttons. The set
point is 100°C if the display shows “HI.” The last
entered set point is stored in memory.
Change run time or heater time set point:
To change the time set point, press the set point
“SELECT” button until arrows point to the run time set
point which can be changed from 1 to 999 minutes by
pressing the increase or decrease buttons. If it is
desired to have the CentriVap run continuously without
alarming at the end of a time period, press the increase
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Chapter 4: Using Your CentriVap
button until the display says “ON.” The last entered set
point is stored in memory.
If during a run the “STOP” button is pressed, the timers
remember the time at which stop occurred. If “RUN” is
then pressed, the timers continue to count down from
the time at which they were stopped. If you are running
program 1, program 2 or program 3, pressing “PROG
1”, “PROG 2” or “PROG 3” resets the timers to the
original set point time and the CentriVap starts a new
run.
To reset the timers to the original set point time when
running programs other than program 1, program 2 or
program 3, use the “SELECT” button to select the
program display and change the program.
Operating The CentriVap
Never lift the lid if the rotor is moving.
!!!!
1. Select a program or set the set point parameters.
2. Preheat the chamber if desired.
3. Place samples in vials. Normally the vials should
be no more than half full. Place vials in a rotor.
4. Load the rotor with samples into the chamber.
5. Close the lid.
Never attempt to reach into the
chamber if the rotor is moving.
Never attempt to defeat the lid latch
mechanism.
Do not operate the CentriVap if the
lid is scratched or nicked, or shows
signs of damage.
Never lean on the CentriVap when it
is operating.
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Chapter 4: Using Your CentriVap
6. Press “RUN.” If the display was showing set point
parameters, it will change to show actual
parameters. Press “RUN” again. The “S” (STOP)
in the display changes to “R” (RUN). The program
on the display alternately displays “R” or the
number of the program that is running. If you
intend to run program 1, 2 or 3, simply press
“PROG 1”, “PROG 2” or “PROG 3” to quick start
the CentriVap.
7. Set point parameters can be altered at any time
during a run by first selecting the parameter using
the set point “SELECT” button and then pressing
the “INCREASE” or “DECREASE” switch.
8. If the time set point is used, at the end of the set
time an alarm sounds. All functions cease.
9. Press “STOP” to terminate operation if the
CentriVap has not already stopped itself.
10. When the evaporation is complete, allow the rotor
to stop moving, lift the lid and remove the samples.
Operational Notes
The LCD display conveys various pieces of
information. When the CentriVap is turned on, the
CentriVap returns to the same mode (“RUN” or
“STOP”) that it was in when the power was turned off.
If the CentriVap was in the “RUN” mode when the
power was turned off, when the power is turned on, the
CentriVap attempts to return to the programmed set
points and continue the run.
To reset the timers to the original set point when
running programs other than program 1, program 2 or
program 3, press the start button and hold it for five
seconds. The display will indicate that the timer is
reset.
Interrupting a Cycle After it Has Begun
At any time during a run, the cycle may be stopped by
pressing the “STOP” button. This shuts off all
operating functions. After the rotor stops, the lid may
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Chapter 4: Using Your CentriVap
be opened. If it is necessary to re-start the CentriVap,
close the lid and press “RUN.” The CentriVap resumes
operation at the same set point parameters and the timer
continues to count down from the time at which the
CentriVap was stopped.
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Under normal operation, the CentriVap requires little
maintenance. The following maintenance schedule is
recommended:
As needed:
1. Clean up all spills; remove liquids from the
2. Clean lid and gasket using soft cloth, sponge or
3. At the end of a run, liquid collected in the glass
4. If the media in the optional clear canister has
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chamber. Clean or decontaminate all surfaces using
agents suitable for the substances spilled.
chamois and a mild, non-abrasive soap or detergent.
traps should be removed. To empty a trap, unscrew
the glass bowl from the head. Dispose of the liquid
in accordance with all codes. Reinstall the glass
bowl by screwing it back on to the head.
changed color, discard and replace the insert with a
new insert. For the radiochemical trap insert, no
indicator exists; therefore, it should be discarded
after each use. In radioactive applications, the
system should be monitored with a Geiger counter.
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Chapter 5: Maintaining Your CentriVap
Daily:
1. To prolong the life of the DNA vacuum pump and
to optimize its performance, it is important to purge
all liquid from within it. When the DNA
concentration process is completed, open the
CentriVap lid and push the rotor switch ON. In ten
seconds, the pump will operate and will draw air
through the system. The rotor will not turn with the
lid open. The length of the liquid purge time is
dependent on the volatility of the solvents used.
Use of water may require an overnight purge time.
Monthly:
1. The rubber components on the CentriVap may
eventually deteriorate and require replacement. The
effective life of rubber parts depends upon both
their usage and the surrounding environment.
Check all rubber hoses and gaskets and replace any
that show signs of hardening, permanent set or
deterioration.
2. Using a soft cloth, sponge or chamois and a mild,
non-abrasive soap or detergent, clean the glass lid.
3. Using a soft cloth, sponge, or chamois and a mild,
non-abrasive soap or detergent, clean the exterior
surfaces of the unit. Liquid spray cleaners and
polishes may be used on the exterior surfaces. Do
not use solvents to remove stains from the exterior
surfaces as they may damage the finish.
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The configuration of your CentriVap can be changed to
accommodate your needs. If the solvents used in the
CentriVap degrade the acrylic lid it may be replaced
with an optional glass lid to gain added chemical
resistance. You may wish to add a secondary trap to
trap vapors exhausted from the vacuum pump. To
observe the samples while they are processing, an
optional strobe light may be installed. Read this
chapter to learn how to:
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• install a glass lid
• install a chemical trap.
• install a CentriZap strobe.
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Installing a Glass Lid
Turn off the CentriVap. Unplug the power cord from
the wall receptacle. Pull outward on the hinge pin
knob. Lift and remove the old lid. To install the new
lid, reverse the process.
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Chapter 6: Modifying Your CentriVap
Installing a Secondary Chemical Trap
An accessory secondary chemical trap is available to
minimize the exhausting of solvents into the
atmosphere. Attach the bracket to the LH side of the
housing using the screws provided. Attach the hose
from the outlet on the vacuum pump to the “out”
connector of the canister housing. Unscrew the clear
bowl of the canister housing from the head. Remove
both the upper and lower caps from the filter cartridge
and insert the small end of the cartridge into the hole in
the center of the head. Reinstall the clear bowl.
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Clear
Cannister
Clamp Hose
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Bracket
Chapter 6: Modifying Your (Product Name)
Be sure to use the proper cartridge for your application.
The moisture cartridge is used to trap water vapor. The
acid cartridge is used to trap acid vapors. The solvent
cartridge is used to trap solvent vapors and the
radiochemical cartridge is used to trap radioactive
waste.
When the media in the insert has changed color, discard
the insert and replace it with a new insert. For the
radiochemical trap insert, no color indicator exists,
therefore it should be discarded after each use. Use a
Geiger counter to monitor the pump exhaust.
THIS RADIOCHEMICAL
☞
CARTRIDGE DOES NOT MEET
NRC FILTER DESIGN
RECOMMENDATIONS.
AFTER OPERATING, PROPERLY
DISPOSE OF ALL HAZARDOUS
MATERIALS IN COMPLIANCE
WITH ALL APPLICABLE CODES.
LABCONCO IS NOT RESPONSIBLE
FOR IMPROPER DISPOSAL OF ANY
MATERIALS.
Installing a CentriZap
Strobe Light
An accessory strobe light is available to enable you to
see the samples as they are rotating in the rotor. Attach
the holder to the right hand side of the CentriVap using
the screws provided. Plug the connector on the strobe
light harness into the receptacle on the back of the
CentriVap marked “STROBE OUTLET.” Peel the
protective backing off the enclosed Velcro® and attach
it to the back of the vacuum pump housing. Secure the
power supply box on the harness to the Velcro. Place
the light in the holder.
To use the strobe light while the CentriVap is operating,
remove the light from its holder, press the trigger and
shine the light on the samples in the rotor.
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Chapter 6: Modifying Your CentriVap
Holder
Screws
Plug wire into
“Strobe” receptacle
Box secured
with Velcro
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Refer to the following if your CentriVap fails to operate
properly. If the suggested corrective actions do not
solve your problem, contact Labconco for additional
assistance. The following failure codes may appear on
the display when problems are sensed by the internal
self check routine.
DISPLAY
ERROR CODE
HEAT SENSOR
CLOSE LID
LATCH FAIL
CAUSE CORRECTIVE ACTION
Sensor failure Replace sensor assembly.
Connection failure Repair connection.
Lid open Close lid.
Solenoid failure Check component.
Switch or sensor failure Check connections.
G
MEM FAIL P1
MOTOR ERROR
Product Service Domestic 1-800-522-7658, International 816-333-8811
Memory failure Push program button #1
New memory IC chip Push program button #1.
Bad memory IC chip Call Labconco – Replace IC chip or
control PCB.
Defective motor Replace motor.
Hall effect sensor failure Replace sensor.
Wire failure Repair wire.
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Chapter 7: Troubleshooting
PROBLEM CAUSE CORRECTIVE ACTION
Other corrective actions for potential problems are as
follows:
Unit will not
operate
Excessive
vibration
Sample odor in
lab
Evaporation
rate is reduced
No vacuum
poor vacuum
Unit not connected to
electrical power
Circuit breaker blown Correct electrical problem and reset
Lid open Close lid.
Sample tubes not
located symmetrically
in rotor
Vent hose exhausting
into lab area
Heater inoperable Contact Labconco.
Vacuum pump failure Check pump.
Pump not on Turn on pump.
Liquid in glass trap Empty trap.
Control valve open Check control valve.
Connect unit to proper electrical
receptacle.
circuit breaker by pressing button.
Reposition sample tubes.
Redirect hose to fume hood.
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Leaks in lines or
connectors or gasket
Foreign material on lid
gasket
Locate and repair.
Clean gasket and lid.
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The following pages list components that are available
for your CentriVap. The parts shown are the most
common replacement parts. If other parts are required,
contact Product Service.
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Appendix A: CentriVap Components
CentriVap Components
Item Quantity Part No. Description
1 1 7459000 Motor w/Capacitor
2 1 7481700 Valve, Vacuum Break (115V)
2A 1 7481701 Valve, Vacuum Break (230V)
3 1 7453500 Heater (115V)
3A 1 7453501 Heater (230V)
4 1 1647106 O-Ring, Outer
5 1 1647105 O-Ring, Inner
6 1 7975902 Fuse, High Temp Limiter
7 1 7456000 Harness, Temp & Speed Sensor
8 1 7479400 PCB
9 1 1289200 Relay (115V)
9A 1 1289100 Relay (230V)
10 1 7826000 Switch, Latch
11 1 7478700 Solenoid, Latch
12 1 7403701 Display
13 1 7455300 Label/Switch Pad
14 1 7452600 Gasket, Lid
15 1 7452500 Lid, Acrylic
16 42.2 inch 7451700 Extrusion
17 2 7452200 Hinge, Bracket Assembly
18 1 1289300 Circuit Breaker (115V)
18A 2 1289308 Circuit Breaker (230V)
19 1 7450400 Vacuum Pump (115V)
19A 1 7450401 Vacuum Pump (230V)
20 2 7450200 Jar, Trap
20A 2 7450300 Gasket, Jar, Trap
Not Shown 1 1334500 Power Cord (115V)
Not Shown 1 1334100 Power Cord (230V)
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Appendix A: CentriVap Components
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Appendix A: CentriVap Components
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Appendix B: CentriVap Dimensions
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This Appendix contains technical information about the
CentriVap including specifications, environmental
operating conditions, wiring diagrams and evaporation
rates.
Electrical Specifications
• Nominal amperage for 115V CentriVap (model
79700-00) (including vacuum pumps): 5.5A
• Nominal amperage for 230V CentriVap (model
79700-01) (including vacuum pumps): 2.8A
• Frequency (all models): 50/60 Hz
• Phase: Single
• Heater power: 300 watts
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Environmental Conditions
• Indoor use only.
• Maximum altitude: 6562 feet (2000 meters).
• Ambient temperature range: 41° to 104°F (5° to
40°C).
• Maximum relative humidity: 80% for temperatures
up to 88°F (31°C), decreasing linearly to 50%
relative humidity at 104°F (40°C).
• Main supply voltage fluctuations not to exceed
±10% of the nominal voltage.
• Transient overvoltages according to Installation
Categories II (Overvoltage Categories per IEC
1010). Temporary voltage spikes on the AC input
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Appendix C: CentriVap Specifications
• Used in an environment of Pollution degrees 2 (i.e.,
line that may be as high as 1500V for 115V models
and 2500V for 230V models are allowed.
where normally only non-conductive atmospheres
are present). Occasionally, however, a temporary
conductivity caused by condensation must be
expected, in accordance with IEC 664.
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Appendix C: CentriVap Specifications
Wiring Diagram (115V Model)
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Appendix C: CentriVap Specifications
Wiring Diagram (230V Model)
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Evaporation Rates
Methylene Chloride
Tube Size
(ml)
50 12 25 45 80 3.75
15 18 10 45 45 4.00
Number of
Samples
Sample
Size (ml)
Appendix C: CentriVap Specifications
Rate
Heater
Temp (C)
Time to
Dry (min)
Overall
(ml/min)
Toluene
Acetonitrile
Methanol
Water
50 12 25 45 204 1.47
50 12 25 100 96 3.13
15 18 10 45 119 1.51
15 18 10 100 42 4.29
50 12 25 45 184 1.63
50 12 25 100 81 3.70
15 18 10 45 106 1.70
15 18 10 100 52 3.46
1.5 132 1 45 55 2.40
1.590145471.92
1.5 132 1 60 37 3.57
1.5 132 1 75 33 5.74
1.5 132 1 100 21 6.29
50 12 25 45 233 1.29
15 18 10 45 141 1.28
1.5 132 1 35 88 1.50
1.5 132 1 45 64 2.06
1.5 132 1 60 50 2.64
1.590175382.37
1.5 132 1 75 39 3.38
1.5 132 1 100 25 5.28
50 12 25 45 1397 0.21
50 12 25 100 445 0.67
15 18 10 45 841 0.19
1.5 132 1 45 427 0.30
1.5 132 1 60 303 0.44
1.5 132 1 75 196 0.67
1.5 132 1 100 117 1.13
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Appendix C: CentriVap Specifications
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The following accessories are available for the CentriVap
DNA System.
PART # DESCRIPTION
74609-00 Clear Canister
78148-00 Acid Trap Insert
78149-00 Moisture Trap Insert
78150-00 Radiochemical Trap Insert
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78152-00 Solvent Trap Insert
74566-01 Glass Lid for Concentrator
74643-00 Strobe Light
74619-00 Rotor – Microtiter 96 Well Plate
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Appendix D: CentriVap Accessories
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