Labconco 7970010, 7970011 Instruction Manual
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Congratulations on your purchase of a Labconco
CentriVap DNA Concentrator System. Models are
available for operation on 115V or 230V.
The CentriVap Concentrator uses centrifugal force with
heat and vacuum to rapidly dry precipitates of DNA
and RNA samples. Centrifugation eliminates bumping
and foaming as vacuum is applied and also concentrates
the solute in the bottom of the vial. This allows
recovery of solutes from sample volumes as small as a
few microliters. The Concentrator is equipped with a
300 watt heater to speed evaporation by warming the
chamber during processing. A timed outlet for the
vacuum pump connection delays the start of the pump
until the rotor reaches operating speed. A safety switch
prevents the concentrator from starting when the lid is
in the open position and a latch prevents the lid from
being opened while the rotor is turning. The compact,
low profile design allows the Concentrator to be placed
on a bench top or other laboratory work surface.
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All wetted parts within the pump are either Teflon*,
Teflon coated or stainless steel. A glass trap is
provided to remove liquid from the system before it
enters the pump and a second glass trap is provided on
the outlet side of the pump to trap liquid expelled from
the pump.
*Teflon® is a registered trademark of DuPont
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Chapter 1: Introduction
The microprocessor which controls the operation of the
heater and the motor provides excellent regulation and
reproducibility of protocols. The end of the run can be
signaled by a manually preset timer. Nine different
protocols can be stored in memory so exact test
parameters can be easily repeated.
About This Manual
This manual is designed to help you learn how to
install, use, and maintain your CentriVap. Instructions
for performing routine maintenance and making minor
modifications to your CentriVap are also included.
Chapter 1: Introduction provides a brief overview of
the CentriVap, explains the organization of the manual,
and defines the typographical conventions used in the
manual.
Chapter 2: Prerequisites explains what you need to do
to prepare your site before you install your CentriVap.
Electrical and exhaust requirements are discussed.
Chapter 3: Getting Started contains the information
you need to properly unpack, inspect and install your
CentriVap.
Chapter 4: Using Your CentriVap discusses the basic
operation of your CentriVap. Information on how to
arrange the samples inside your CentriVap and select an
operating cycle is included.
Chapter 5: Maintaining Your CentriVap explains how
to perform routine maintenance on your CentriVap.
Chapter 6: Modifying Your CentriVap describes how to
install the optional glass lid.
Chapter 7: Troubleshooting contains a table of
problems you may encounter while using your
CentriVap, including the probable causes of the
problems, and suggested corrective actions.
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Appendix A: CentriVap Components contains labeled
diagrams of the components of the CentriVap.
Appendix B: CentriVap Dimensions contains
comprehensive diagrams showing the dimensions for
the CentriVap.
Appendix C: CentriVap Specifications contains product
specifications. Wiring diagrams for both the 115V and
230V CentriVap are also included.
Appendix D: CentriVap Accessories lists the part
numbers and descriptions of all of the accessories
available for your CentriVap.
Typographical Conventions
Chapter 1: Introduction
Recognizing the following typographical conventions
will help you understand and use this manual:
• Book, chapter, and section titles are shown in italic
type (e.g., Chapter 3: Getting Started).
• Steps required to perform a task are presented in a
numbered format.
• Comments located in the margins provide
suggestions, reminders, and references.
• Critical information is presented in boldface type in
paragraphs that are preceded by the exclamation
icon. Failure to comply with the information
following an exclamation icon may result in injury
to the user or permanent damage to your CentriVap.
• Important information is presented in capitalized
type in paragraphs that are preceded by the pointer
icon. It is imperative that the information contained
in these paragraphs be thoroughly read and
understood by the user.
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Chapter 1: Introduction
Your Next Step
If your CentriVap needs to be installed, proceed to
Chapter 2: Prerequisites to ensure your installation site
meets all of the requirements. Then, go to Chapter 3:
Getting Started for instructions on how to install your
CentriVap and make all of the necessary connections.
For information on the operational characteristics of
your CentriVap, go to Chapter 4: Using Your
CentriVap.
If your CentriVap is installed and you need to perform
routine maintenance on the CentriVap, proceed to
Chapter 5: Maintaining Your CentriVap.
For information on making modifications to the
configuration of your CentriVap go to Chapter 6:
Modifying Your CentriVap.
Refer to Chapter 7: Troubleshooting if you are
experiencing problems with your CentriVap.
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Before you install your CentriVap, you need to prepare
your site for installation. Carefully examine the
location where you intend to install your CentriVap.
You must be certain that the area is level and of solid
construction. In addition, an exhaust means must be
provided. An electrical source must be located near the
installation site.
Carefully read this chapter to learn:
• the electrical supply requirements.
• the exhaust requirements.
Refer to Appendix C: CentriVap Specifications for
complete CentriVap electrical and environmental
conditions, specifications and requirements.
The CentriVap has not been evaluated
by an approval agency for the use of
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biological, radiotoxins or flammable
liquids or materials.
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Electrical Requirements
A dedicated grounded electrical outlet is required. This
outlet requires a 15 Amp circuit breaker or fuse for
models rated at 115V (60 Hz). An 8 Amp circuit
breaker or fuse is required for models rated at 230V
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Chapter 2: Prerequisites
(50/60 Hz). 115V models are equipped with a 15 Amp
NEMA 5-15P plug. 230V models are not equipped
with a plug. It will be necessary to install a plug to
match the available receptacle.
It is recommended that an emergency switch for
disconnecting the mains in the case of a malfunction be
located remote from the CentriVap, preferably outside
the room in which the CentriVap is housed, or adjacent
to the exit from that room.
Location and Exhaust Requirements
The CentriVap DNA System should be located within a
fume hood if hazardous or flammable solvents are used.
In all cases, regardless of the solvent used, it is strongly
recommended that the vacuum pump is vented in a
fume hood. An optional accessory secondary trap is
available to minimize the exhausting of solvents into
the atmosphere. This does not, however, negate the
need to exhaust the vacuum pump into a fume hood.
Space Requirements
Refer to Appendix C: CentriVap Specifications for
dimensional drawings of the CentriVap.
Your Next Step
After you have determined that the location for your
CentriVap accommodates the installation and
operational requirements, you are ready to unpack and
install your CentriVap. Proceed to Chapter 3: Getting
Started.
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Now that the site for your CentriVap is properly
prepared, you are ready to unpack, inspect, install, and
test your CentriVap. Read this chapter to learn how to:
• unpack and move your CentriVap.
• set up your CentriVap.
• connect the electrical supply source to your
CentriVap.
• properly exhaust your CentriVap.
• safely use solvents with your CentriVap.
The CentriVap weighs over 69 lbs.
(31 Kg). The carton allows for lifting
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with a mechanical lift truck or hand
truck. If you must lift the CentriVap
manually, use at least two (2) persons
and follow safe lifting guidelines.
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Chapter 3: Getting Started
Unpacking Your CentriVap
The United States
Interstate Commerce
Commission rules
require that claims be
filed with the delivery
carrier within fifteen (15)
days of delivery.
Carefully unpack your CentriVap and inspect it for
damage that may have occurred in transit. If your
CentriVap is damaged, notify the delivery carrier
immediately and retain the entire shipment intact for
inspection by the carrier.
DO NOT RETURN GOODS
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WITHOUT THE PRIOR
AUTHORIZATION OF LABCONCO.
UNAUTHORIZED RETURNS WILL
NOT BE ACCEPTED.
IF YOUR CENTRIVAP WAS
DAMAGED IN TRANSIT, YOU
MUST FILE A CLAIM DIRECTLY
WITH THE FREIGHT CARRIER.
LABCONCO CORPORATION AND
ITS DEALERS ARE NOT
RESPONSIBLE FOR SHIPPING
DAMAGE.
Do not discard the carton or packing material for your
CentriVap until you have checked all of the
components and installed and tested the CentriVap.
CentriVap Components
As previously mentioned, the CentriVap DNA System
is available in 115V or 230V. Locate the model of
CentriVap you received in the following table. Verify
that the components listed are present and undamaged.
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Catalog # Product Description
79700-00 DNA Concentrator – 115V
79700-01 DNA Concentrator – 230V
Plus the Following:
Part # Component Description
74572-00 User’s Manual
13345-00
13341-00
74629-00 Rotor
If you do not receive one or more of the components
listed for your CentriVap, or if any of the components
are damaged, contact Labconco Corporation
immediately for further instructions.
Power Cord – 115V
or
Power Cord – 230V
Setting Up Your CentriVap
Chapter 3: Getting Started
After you verify receipt of the proper components,
move your CentriVap to the level and sturdy location
where you want to install it. There is no need to secure
the CentriVap DNA System to the work surface. Then,
follow the steps listed below.
Emergency Access Into the
Chamber
The CentriVap is designed to prevent access to the
chamber in the event of a power disruption. If it is
necessary to open the lid when there is no electrical
power connected to the CentriVap, insert a small
screwdriver or similar instrument into the small round
hole on the left side of the case near the top behind the
control panel. This will unlock the lid latch
mechanism. While holding the screwdriver in place,
raise the lid with the other hand.
Never attempt to defeat the latch or
open the lid while the CentriVap is
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running.
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Chapter 3: Getting Started
Exhaust Port
If the CentriVap is not located in a fume hood, attach
one end of a .50 inch inside diameter hose to the
exhaust port on the left side on the vacuum pump glass
trap exhaust port. Clamp securely. Route the other end
to a fume hood or other laboratory ventilation device.
Electrical Connection
Plug the power cord into the receptacle on the back of
the CentriVap and plug the other end into a suitable
power receptacle.
Rotor Installation
Place the rotor onto the shaft of the Concentrator.
Rotate the rotor slightly to engage the drive pin in the
shaft with the slots in the rotor hub. The top of the
shaft should be in line with the top of the rotor hub.
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Do not use a rotor if it shows any signs
of damage.
Solvent Safety Precautions
The CentriVap is not classified as
“explosion proof.” It has been
designed with safety as a primary
consideration
prudent manner using “good
laboratory practices.” It has been
designed for use with compounds as
described in the United States
National Electrical Code Class I,
Group D. The heater may be
programmed to run as hot as 100°C,
however, the heater element may
normally run at 110°C. A thermal
fuse limits the heater to a maximum
temperature of 141°C. It is important
and should be used in a
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that the solvents used are compatible
with these temperatures. Do not
evaporate solvents that have an
autoignition temperature below
180°C. Do not evaporate solvents that
are classified as Group A, B, or C by
the National Electrical Code.
Evaporate only non-flammable or
Group D solvents with autoignition
temperatures 180°C or above. Use of
other compounds could cause an
explosion.
Solvents used in the CentriVap may
be flammable or hazardous. Use
extreme caution and keep sources of
ignition away from the solvents.
When using flammable or hazardous
solvents, both the CentriVap and the
vacuum pump should be operated
inside a fume hood.
Chapter 3: Getting Started
If a sample is spilled in the chamber it
must immediately be cleaned up.
Hazardous materials such as strong
acids or bases, radioactive substances
and volatile organics, must be handled
carefully and promptly cleaned up if
spilled.
Do not store flammable or hazardous
solvents within 12 inches (300 mm) of
the CentriVap.
Several components of the CentriVap
which are located inside the chamber
are made of stainless steel which can
be attacked by acids. Use of acids
such as trifluoroacetic acid can result
in degradation of the product and the
vacuum pump if the vacuum system is
being used. Use care when using
aggressive liquids which can damage
the CentriVap and thoroughly clean
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Chapter 3: Getting Started
the CentriVap after each use. Contact
Labconco before evaporating acids.
WARNING: The disposal of
substances used in connection with
this equipment may be governed by
various Federal, State or local
regulations. All users of this
equipment are urged to become
familiar with any regulations that
apply in the user’s area concerning
the dumping of waste materials in or
upon water, land or air and to comply
with such regulations.
Your Next Step
The installation and setup of your CentriVap is now
complete. To learn how to load and operate your
CentriVap, proceed to Chapter 4: Using Your
CentriVap. To make a modification to the
configuration of your CentriVap, proceed to Chapter 6:
Modifying your CentriVap. To perform additional
diagnostics on your CentriVap, proceed to Chapter
7:Troubleshooting. To learn about the maintenance
requirements for your CentriVap, proceed to Chapter 5:
Maintaining Your CentriVap.
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Product Service Domestic 1-800-522-7658, International 816-333-8811
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After your CentriVap has been installed as detailed in
Chapter 3: Getting Started, you are ready to begin
using your CentriVap. Read this chapter to learn how
to:
• set operating parameters.
• operate the controls.
• properly select and position glassware inside
• understand the display.
• interrupt a cycle after it has begun.
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your CentriVap.
Do not use the CentriVap in a manner
not specified by the manufacturer
(refer to Appendix C: CentriVap
Specifications). The electrical
protection properties of the CentriVap
may be impaired if the CentriVap is
used inappropriately.
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Do not store or stack supplies or
equipment on top of the CentriVap.
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